Separation Science – Uppsala University

RESEARCH In the Border Between Analytical Chemistry, Physical Chemistry and Chemical Engineering

PhD Students: Robert ArnellJörgen Samuelsson

Group Leader:Torgny Fornstedt

Torgny.Fornstedt@ytbioteknik.uu.se070-425 06 83

Research Profile Preparative LC Theory

Computer Simulations

New Methods for Determination of Adsorption Isotherms

Characterization of New Columns (Adsorption Energy Distribution calculations) Preparative CSPs HPLC Phases for Alkaline

Conditions

Tuning of Band Shapes for Increased Production (Chirobiotic T, CHI-TBB?)

1. Analytical Reason:A. Column Characterization

Capacity, Selectivity

Surface Heterogeneity

B. Detailed Characterization of Drug-Protein Interactions using Immobilized Protein Stationary Phases

Affinity, Enantioselectivity

Displacement Studies

Multi-Site Interaction

Why measure Adsorption Isotherms?

Adsorption Isotherms Known

Chromatography Simulation

Numerical Process Optimization(SMB)

Why measure Adsorption Isotherms?

2. Process Related Reason:

Publications 20061. An Improved Algorithm for Solving Inverse Problems in Liquid

Chromatography. P. Forssén, R. Arnell and T. Fornstedt. Computers & Chemical Engineering (2006) 30, 1381-1391.

2. Discovery of invisible extra fronts in single-component frontal analysis in liquid chromatography by J. Samuelsson and T. Fornstedt. Journal of Chromatography A (2006) 1114, 53-61.

3. Analytical Characterization of Chiral Drug-Protein Interactions: Comparison between an SPR Assay and the HPLC Perturbation Method. R. Arnell, N, Ferraz and T. Fornstedt. Analytical Chemistry (2006) 78 1682-1689.

4. Invisible Analyte Peak Deformations in Single-Component Liquid Chromatography” by J. Samuelsson, R. Arnell and T. Fornstedt. Analytical Chemistry (2006) 78 2765-2771.

5. Validation of the Tracer Pulse Method for Multi Component Liquid Chromatography- a Classical Paradox Revisited. R. Arnell and T. Fornstedt. Analytical Chemistry (2006) 78, 4615-4623.

6. General theory of indirect detection in chromatography. P. Forssén and T. Fornstedt. Journal of Chromatography A (2006) 1126, 268-275.

OUR GROUP!

From left: Robert Arnell, Jörgen Samuelsson and T. Fornstedt

Patrik Forssén

Dept. Of Scientific

Computing

Se vår kurs industrin: HPLC teori för praktiskt bruk,Flera tillfällen tidigt i vår, kommer upp snart på:http://www.kemi.uu.se/industrikurser/LC-teori/index.shtm