GENReports:,Market,&,Tech,Analysis,!Produced!by!Oosta!and!Razvi!©!2015,GENengnews.com,

> Enal Razvi, Ph.D. > Gary Oosta, Ph.D. Managing Director Select Biosciences, Inc. E-mail: enal@selectbio.us Twitter: @enalrazvi!

Biotechnology Analyst, Select Biosciences, Inc.

GENReports: Market & Tech Analysis

Single,Cell,Analysis,(SCA):,

Expanding,in,Importance,in,

Life,Science,Research,–,

circa,2015,

GENReports:,Market,&,Tech,Analysis,!Produced!by!Oosta!and!Razvi!©!2015,GENengnews.com,

Single,Cell,Analysis,(SCA),in,the,Life,Sciences,

Marketplace,Analyzed,from,the,BoNomOUp,

Scope,of,this,Report:!

!  There!is!the!unmet!need!to!characterize!the!dynamics!of!the!single!cell!analysis!in!the!life!sciences!marketplace!since!such!an!analysis!will!provide!an!en#bloc#view!of!the!field!which!is!known!to!be!“amorphous”!as!it!impact!many!different!applicaEon!areas!

!  Given!the!nascent!nature!of!this!field,!we!believe!that!an!excellent!means!to!analyze!this!space!is!to!view!the!enEre!body!of!academic!literature!published!in!this!field!–!in!this!manner,!viewing!the!“niches”!that!are!currently!expanding!as!well!as!technologies/methodologies!driving!the!space!forward!

!  We,analyzed,28,401,publicaVons,in,total,that,together,consVtute,the,bulk,of,

the,academic,literature,in,this,field,at,the,present,Vme,(August,2015),

!  These,publicaVons,represent,the,period,2010"2015YTD,and,therefore,represent,the,most,

recent,5Oyears,of,publicaVon,acVvity,in,this,field,

!  The,analysis,presented,in,this,report,are,based,on,Single,Cell,AnalysisOfocused,publicaVons,

from,this,period,2010"2015YTD,

GENReports:,Market,&,Tech,Analysis,!Produced!by!Oosta!and!Razvi!©!2015,GENengnews.com,

Data,Mining,and,Technology,AnalyVcs,Reveal,

Technology,and,Market,Trends,in,SCA,

Categorize!and!Segment!using!

Comprehensive!Database:!!

28,401,papers,on,SCA,

The!Model!

Hotspot!Map!

850!Segments,!Categories!&!Model!!(Keywords/Phrases)!

Broad!!Search!

Graphs!Rank segment new+vp+val1 EXOSOME 1.0002 OCCULT.&.CANCER 1.0003 GYNECOLOGIC.&.CANCER 0.8904 AMYOTROPHIC.LATERAL.SCLEROSIS 0.8545 CLOSTRIDIUM.DIFFICILE 0.8546 ADENOCARCINOMA.&.CANCER 0.8357 FACTOR.VIII 0.7818 MYOBLAST 0.7819 ROTAVIRUS,.REOVIRUS 0.78110 SCREEN&PRINT 0.75511 GLIOBLASTOMA.&.CANCER 0.71112 AST/SGOT 0.67613 CORTISOL 0.67614 HEAD?AND?NECK.&.CANCER 0.67615 LIVER.TRANSPLANT 0.67616 PARKINSON'S,.PARKINSONS,.GAIT.DISORDER 0.67617 PAPER+BASED 0.67418 SOFT.TISSUE.&.CANCER 0.66019 OVARIAN.&.CANCER 0.656

Tables!

Reveals!Trends!from!the!BoWomXUp!

+!Experience!from!past!projects!

Literature!&!Process!Flow!Charts! +! Adjacent!Word!

keyword!expansion!+!MarkeEng!Research! +!

Text!Clustering!

GENReports:,Market,&,Tech,Analysis,!Produced!by!Oosta!and!Razvi!©!2015,GENengnews.com,

Single,Cell,Analysis,Database,from,PubMed,has,28,401,papers,in,the,

period,2010"2015YTD.,

The,Word,Cloud,from,Vtles,hints,at,the,topics,found,in,these,papers.,

GENReports:,Market,&,Tech,Analysis,!Produced!by!Oosta!and!Razvi!©!2015,GENengnews.com,

Single,Cell,Analysis,Papers,from,PubMed,

To,see,the,most,recent,developments,more,clearly,,we,analyzed,publicaVons,

from,2010"2015YTD,[highlighted,in,Red],

Year,of,PublicaVon,

GENReports:,Market,&,Tech,Analysis,!Produced!by!Oosta!and!Razvi!©!2015,GENengnews.com,

Fluorescence,is,the,Dominant,enabling,technology,in,SCA,,

but,noVce,the,Upsurge,in,Mass,Spectrometry,Recently,

GENReports:,Market,&,Tech,Analysis,!Produced!by!Oosta!and!Razvi!©!2015,GENengnews.com,

Imaging,methods,are,important,to,SCA.,,,Flow,cytometry,is,a,

Growth,Area.,,An,InteresVng,“new”,Area,is,Molecular,LocalizaVon,

Microscopy,

GENReports:,Market,&,Tech,Analysis,!Produced!by!Oosta!and!Razvi!©!2015,GENengnews.com,

Microfluidics,for,SCA,is,Expanding,",A,Very,Clear,

Trend,

The,Use,of,Microfluidics,for,Single,Cell,Capture,and,Analyses:,

An,Important,and,Growing,Space,

GENReports:,Market,&,Tech,Analysis,!Produced!by!Oosta!and!Razvi!©!2015,GENengnews.com,

PCR,and,qPCR,are,the,dominant,assay,type,in,SCA",InterrogaVon,

of,SCA,Nucleic,Acid,Cargo,,

IsolaVon,Methods,are,Quite,Limited,Currently,

GENReports:,Market,&,Tech,Analysis,!Produced!by!Oosta!and!Razvi!©!2015,GENengnews.com,

Arrays/Microarrays,Have,Penetrance,in,the,SCA,Space,as,Means,

of,Studying,“Known,GeneVc,Elements”,in,Material,from,Single,

Cells,

GENReports:,Market,&,Tech,Analysis,!Produced!by!Oosta!and!Razvi!©!2015,GENengnews.com,

A,Variety,of,Cells,and,Tissues,are,MenVoned,in,the,SCA,Database,",This,

suggests,a,Heterogeneous,Set,of,Samples/StarVng,Materials,used,in,SCA,

Studies,

GENReports:,Market,&,Tech,Analysis,!Produced!by!Oosta!and!Razvi!©!2015,GENengnews.com,

SCA,intersects,Many,Standard,and,Growth,Technologies.,,NoVce,

the,Rapid,Growth,of,CirculaVng,Tumor,Cells,(CTCs),in,the,SCA,

Space,

CTCs,

These,Spaces,Not,Impacted,by,SCA,Currently,

GENReports:,Market,&,Tech,Analysis,!Produced!by!Oosta!and!Razvi!©!2015,GENengnews.com,

•  SCA,publicaVons,grew,at,a,CAGR,of,7.3%,over,the,last,six,

years.,,,The,growth,rate,is,nearly,linear.,

•  Cancer,,geneVcs/genomics,,DNA,,polymorphisms,and,

imaging,are,among,the,larger,segments.,,These,areas,sustain,

growth,in,SCA.,

•  Larger,NEW,growth,drivers,(newly,emerging,areas,to,watch),

include,NGS,,RNA,sequencing,,and,biomarkers,spaces,

represenVng,the,impact,of,SCA,into,the,diagnosVcs,arena,

•  The,growth,of,CTCs,in,the,context,of,SCA,demonstrates,the,

deployment,of,SCA,technologies,and,approaches,in,the,

cancer,diagnosVcs,space,

•  In,terms,of,methodologies,,both,nucleic,acid,analyses,as,well,

as,mass,spectrometry,(for,protein,analyses),are,an,

expanding,theme,in,the,SCA,field,

SCA,is,an,Expanding,Field,

Growth,is,Driven,by,MulVple,Spaces!

GENReports:,Market,&,Tech,Analysis,!Produced!by!Oosta!and!Razvi!©!2015,GENengnews.com,

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