Slide-Uri Sesiune Fiziologie Pt. Studenti (1)

8/13/2019 Slide-Uri Sesiune Fiziologie Pt. Studenti (1)

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Assessing Stress Responses

Katie McLaughlin, Ph.D.

Margaret Sheridan, Ph.D.


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Agenda

1. Basic Principles

2. Autonomic Nervous System

1. Heart rate and heart rate variability

2. Blood pressure

3. Impedance Cardiography

3. HPA Axis

1. Saliva sample collection

2. Saliva labeling and storage


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1. Basic Principles


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Stress Reactivity

We are interested in seeing how each childs

physiology changesas a result of each of the

study tasks

We are also interested in how quickly each

childs physiology recovers from each of the

study tasks


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Baseline TASK Recovery


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2. Autonomic Nervous System


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Autonomic Nervous System


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Impedance cardiography

Blood PressureHeart Rate & Variability


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Electrocardiograph

Heart Rate and Heart Rate Variability (RSA)


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Impedance Cardiography

A measure of cardiac efficiency Stroke volume = the volume of blood leaving

the heart on each beat (~ 80-120 mL)

Cardiac output = Stroke volume * heart rate Pre-ejection period: pure sympathetic

nervous system response

With blood pressure,

can calculate total

peripheral resistance

(MAP / CO) * 80


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Adaptive Responses Maladaptive Responses

Challenge

(Physiologically tough)

Threat/Defeat

(Physiologically weak)

Appraisals:Resourcesexceed Demands

Appraisals:Demands exceedResources

ANS reactivity:

Pre-ejection period

Cardiac output Vasodilation (TPR)

ANS reactivity:

Pre-ejection period

Cardiac output Vasoconstriction (TPR)

Recovery: Quick Recovery: Sluggish

Differentiating Stress Responses


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Blood Pressure

Measures pressure with that circulatingexerts on the arteries

Systolic blood pressure (high)

Pressure when heart iscontracting

Diastolic blood pressure

(low) Pressure when heart is

calm


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Recording Blood Pressure

The operator will control the acquisition andrecording of blood pressure throughout the

session

Both the systolic and diastolic pressure must be

recorded after each reading

Systolic

Diastolic


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3. HPA Axis


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Cortisol

DHEA

Testosterone


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Collecting Saliva

The child should drool into a saliva tube usingthe straws provided

The tube must be filled to the line, with no bubbles

You can pop the bubbles using the straw or by

tapping the tube onto a table


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Adaptive Responses Maladaptive ResponsesAppraisals:Resources

exceed Demands

Appraisals:Demands exceed

Resources

ANS reactivity:

ventricle contractility Cardiac output

Vasodilation (TPR)

ANS reactivity:

ventricle contractility Cardiac output

Vasoconstriction (TPR)

Hormonal: cortisol

DHEA

Testosterone

Hormonal: cortisol

DHEA

Testosterone

Recovery: Quick Recovery: Sluggish

Differentiating Stress Responses


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Thank you!

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Slide-Uri Sesiune Fiziologie Pt. Studenti (1)