slightly acidic electrolyzed water 4Oct16€¦ · The amount of fuel and water consuming for sterilization after CIP were calculated. The sample line including pipe line, tank, pump

Slightly acidic electrolyzedSlightly acidic electrolyzed waterwater

微酸性電解⽔HOCL water

Hi hl O i i d Chl i d Li idHighly Optimized Chlorinated Liquid

微酸性電解水研究所 HOCL inc.

HOCl is a human borne bactericideImmune systemImmunity substance

TT--lymphocytelymphocyte MacrophageMacrophagewanted

Signal substance (From food)Interleukin１

O2･O2- H2O2

(NADPH (MPO)

(SOD)Cl-

(By respiration)Interleukin１

Thermoregulatory center onO2

HOClHOCl(NADPHoxidase)

(MPO) center onhypothalamus

Activation ProstaglandinBactericidal effect

Body temperature rise

Of HOCl is enhanced


NADPH : Nicotinic acid adenine di nucleotide phosphoric acidSOD ： Superoxide dismutaseMPO ：MyeloperoxidaseNeutrophil, Macrophage : kind of white corpuscles

2

The pH dependency of chlorine compound existing ratio

100 rat

eb.

spo

re)2

4

80

100（%

）

cter

icid

al

lue/

B. s

ub

6

60

ng

ratio Bac

（D

-val8

10

20

40

Exi

stin

0

20

Chlorine gasChlorine gas Tri-hallo-methaneTri-hallo-methaneHOCLt

HOCLt

ChlorineChlorine(Cl(Cl22）） Free hypochlorous acidFree hypochlorous acid（（HOCHOCｌ）ｌ） Hypochlorite ion（OCl-）1 3 5 7 9ｐH →

ggwaterwater

4 62 8

Vertical axis : existing ratio of each chemical, horizontal axis : pH of solution


ＨＨ２２２２

HOClHOCl++－－

ＣｌＣｌ２２++

HH22OOe-e-

ＣｌＣｌ--HClHCl

ＨＨ＋＋

2HCl → Cl2 + H2HClHClCl2 + H2O → HCl + HOCl


微酸性電解水生成原理


有鈉電解式有鈉電解式


圖上沒有繼續說的是圖上沒有繼續說的是

HCl + NaOH → NaCl + H2O


還有還有

HOCl + NaOH → NaOCl + H2O


R l ti f +HOCLRegulation of +HOCL（as food additive）（as food additive）

・Official nameOfficial name “ Slightly acidic hypochlorous water”・ｐH 5.0~6.5

・Available chlorine conc. 10〜30 ppm

Ｒ i l HCl & D i k bl・Ｒaw material HCl & Drinkable water


GeneralGeneralGeneralGeneral


◆◆◆

◆◆◆◆◆◆◆◆◆◆


◆◆◆

◆◆◆◆◆◆◆◆◆


Bactericidal effect comparison, between ASEW and SHC

6)

between ASEW and SHC

522．4ppmNa hypochlorite soln.

(SHC)

unt(

log)

4134ppm

rial

cou

310 8ppml

bact

er

2

10．8ppm

urvi

val

10 10 20 30

22ppm (+HOCL)Su

0 10 20 30Holding time(minute) Using B. subtilis spore

Removing of air microbe by HOCL water fog 120 M

80

100 Bacteria

Microbial

60

80 Bacteria

Mold

l count (C

40

CFU

/250L

0

20

air)

0 1 2 3 4 5 6Spraying time(hr) Device : ultrasonic humidifier

Capacity of room：30m3 ACC of HOCL water : 20ppm

Spraying rate : 60ml／hr Spraying rate : 60ml／hr


.

Generation of chlorine gas while bubblingd di H, depending on pH

12spa

ce

1012

n he

ad

68

gas

in

46

hlor

ine

ｐｐｍ

）

airNaOH soln.

2

c. o

f ch （ｐ

02 3 4 5 6

pH

Con

c

pH微酸性電解水研究所 HOCL inc.

Chloroform generation Dependency on chlorine concentrationDependency on chlorine concentration800

ppb) Sample is cow meat.

C t t ti i 5 i t ll600

form

(p Contact time is 5minutes all.

CL

400chlo

rof

of +

HO

C

200rate

d c

Ran

ge o

0

200

Gen

er R

00 100 200

Available chlorine conc.（ppm)


Generating chloroformGenerating chloroformdependency on pHdependency on pH

11

μμｇ）

ｇ）

00..88

ofor

mof

orm

（（μμ

00 44

00..66

d ch

loro

d ch

loro

00..22

00..44

ener

ate

ener

atepH range of +HOCL

0066 77 88 99 1010

Ge

Ge

５

100100μμｌｌ,, 11% bouillon was dosed into % bouillon was dosed into 10 10 ml ml SHC solution(ACC:SHC solution(ACC:3030ppm). ppm). AfterAfter 55 hours, head space gas was analyzedhours, head space gas was analyzed

pHpHAfter After 5 5 hours, head space gas was analyzed hours, head space gas was analyzed for chloroform.for chloroform.


Generated THMs in washing lettuce・+HOCL：available chlorine concentration(ACC) １７ppm, pH6.6 / over flow ・SHC：ACC200ppm, pH9.7 / lettuce 75g + water1.5 l

8080

（（pp

bpp

b））

SHC

6060

THM

sTH

Ms（（

4040

rate

d T

rate

d T

2020

Gen

erG

ener Tap water+HOCL

0000 22 44 66 88 1010

Soaking timeSoaking time（（minutesminutes））Soaking timeSoaking time（（minutesminutes））


Chloroform generation by using disinfectantUnit：ppb ー：no test

disinfectantGarland

chrysanthemum Lettuce Cow round

Unit：ppb, ：no test

No treatment - 9 21Tap water 11 14 -HOCL waterHOCL water

ACC :17ppmACC :17ppm 1616 1010 1717Sodiumhypochlorite

ACC : 100ppm - - 130Sodiumhypochlorite

ACC : 200ppm 23 31 360微酸性電解水研究所 HOCL inc.

Appearance of lettuce after soaking in

S di h hl iSodium-hypo-chlorite 200ppm solution for10 minutes

HOCL water for 10 minutes


Dry residuey


Problem of dry residue

bactericides contained chemicals residue

+HOCL HOCL no

Neutralized SHC NaOH NaOCl yesNaCl HOClNaCl HOCl

SHC NaOH NaOCl yesHOCｌSodium hypo chloride HOCｌ

S AEW HOCl NaCl yesStrong AEW HOCl NaCl yesCl2 HCl


Dry residue

+HOCL SHC

ACC:30ｐｐｍ, ｐＨ６, ３ｍｌ each


Problem of d iddry residue


CertifiedCertifiedbactericidal effectbactericidal effect


The bactericidal effect of HOCL water wasfi d i h i b

General Food poisoning P th i Vi

confirmed against these microbes.

General Food poisoning Pathogenic Virus

E. coliB b ili

Salmonella enteritidisShi ll d i

InfluenzaE. coli O-157B. subtilis(spore)B. cereus(spore)

Vibrio parahaemolyticusStaphylococcus aureusListeria monocytogenesY i i t liti

Shigella dysenteriae SARSNoroShigella sonnei

Shigella flexneri B. coagulans(spore) Yersinia enterocoliticaCampylobacter jejuni

Serratia marcescens

Vibrio choleraeMycobacterium bovis

B hl f l

Campylobacter coli

gg pB. licheniformis(spore)B. stearothermophilus(spore)

Saccharomyses spCandida albicans

Pseudomonas aeruginosaSerratia marcescens Legionella pneumophila

Clostridium botulinum(spore) Salmonella typhimuriumByssochlamys fulva

Acinetobacter baumanniiVibrio alginolyticusSaccharomyses sp.Cladosporium sp. MRSA

Vibrio alginolyticus

Vibrio parahaemolyticus


Bactericidal effect of HOCL water made ofBactericidal effect of HOCL water made ofsea water against Halophile bacterium

bacteriaContacting time

（second）bacteria （）0 10 30 60

Vibrio alginolyticus 6 3 106Vibrio alginolyticus ATCC17749 6.3×106 －－－Vibrio parahaemolyticus 2 0×105 270 －－O4:K6 2.0×105 270 －－Vibrio parahaemolyticus

O3:K68 1.9×104 －－－O3:K68 1.9 10(－)：＜10／ml ACC : 23ppm、pH : 6.5


Sterilization of raw rice

Churning washing for short time with same volume of water as rice(5 times)with same volume of water as rice(5 times)

Soaking for 15 minutes during over flowing

Soaking for 45 minutes

Water Aerobic count （CFU／g）

No treatment 790

HOCL t 0HOCL water 0

Tap water 170


20Temperature dependency of bactericidal speed

time 20

uctio

n te

)15

als

redu

(min

u 10

deci

ma

5

6 d

020 30 40 50 6020 30 40 50 60

Temperature( )B. subtilis spore


60℃ HOCL water treatment（HOCL t ACC 30 H 6 0） b i l CFU/

Water Temp.（℃） Time（s） Coliform Aerobic No treatment －－ 9.0×10 4.8×103Leek

（HOCL water : ACC 30ｐｐｍ、ｐH 6.0） bacterial count : CFU/g

HOCL W. 60 30 0 4HOCL W. 60 60 0 2Tap water 60 60 0 3.2×102

(Welsh onion)

Crispyness was kept even after 30 seconds treatment

Water Temp.(℃) Time（m） Coliform AerobicNo treatment －－ 3.4×104 5.8×105

HOCL W. 60 2 0 9.0×10HOCL W. 60 5 0 9.0×10Tap water 60 2 2 3.2×103

Cucumber

Crispyness was lost partially by 5 minutes treatment,

Water Temp.(℃) Time （s） Coliform Aerobic No treatment －－ 2.7×102 1.7×105No treatment 2.7×10 1.7×10

HOCL W. 60 30 0 1.0×102

HOCL W. 60 60 0 5.8×10HOCL W. 60 180 0 1.0×10

Chinesecabbage

Tap water 60 60 4 1.2×103

Softening took place by 180 s treatment

2. Cooker sterilization test

Contaminated the process components ( marshmellow, candy ) and contaminated

the cooker inside with these.

* Washed the cooker inside with 90°C, hot water for 20 minutes Checked M.O

* Washed the cooker inside with HOCL for 20 minutes Checked M.O

Checked inside with swabbing.

Result)

2.1 Marshmellow Cooker Unit : cfu/10cm²

Control 90°C, H.W HOCL

Aerobic Bacteria

TNTC 480 0

E.Coli TNTC 260 0

Glaucus group TNTC 50 0

Cladosporium TNTC 30 0

2.2 Candy Cooker Unit : cfu/10cm²

Control 90°C, H.W HOCL

Aerobic B t i

TNTC 860 0Bacteria

E.Coli TNTC 520 0

Glaucus group TNTC 30 0

Cladosporium TNTC 40 0Cladosporium TNTC 40 0


StabilityStability


Practically stableIn a shaded reservation tank with rid

y

16

20

12

ｐｍ）

8

C（ｐｐ

0

4AC

0 1 2 3 4 5 6 7

Reserving daysReserving days


Redaction of ACC in an open tank at 70

20

25

15

20pm

)

10

AC

C(p

p

5

A

00 50 100 150

( )Reserving time (minute)


Comparison ith th b t i idwith other bactericides


HOCLwater

StrongAEW

SHC Alcohol

Bactericidal effect almost all almost all insufficient insufficient

Against the virus available available available insufficient Stability practical no practical practical Generating THMs no no serious nogMass generating available no available no Useless water no serious no noUseless water no serious no noDrying residue no yes yes no Causing odd flavor no no serious seriousCausing odd flavor no no serious seriousCausing skin trouble slight weak serious serious


MeritMerit


Reduction of energy and resources The amount of fuel and water consuming for sterilization after CIP were calculated.

The sample line including pipe line, tank, pump etc. needs 1 t water to wash out.

0 012N（） 0.012Nm39Nm3

（Fuel）

Change9Nm3Sterilization Sterilization Sterilization

Change

By HOCL waterBy HOCL water

2（Water amount）

By hot water

1t2tamount）

◆ In case of hot water, 20 water is heated to 120 . Heating time and the loss of heat are ignored. Cooling water is flowed 1t/minute.

◆ Fuel of boiler is methane. Heat generating rate is 11000kcal/m3Ｎ. Cost is 50\/m3N. Thermal efficiency is 100%.◆ Price of water is 200\/ｔ. Energy to consume for water treatment is ignored.◆ Cooling water is heated from 20 to 120 , and cooled to 20 again.

CalculationCondition g , g

◆ Amount of cooling water is twice of hot water. Cooling cost is same as heating cost.◆ Power generation efficiency of methane is 50％.

Condition


Simplification of a process Simplification of a process p pp p

++HOCLHOCLHeating or

other chemical

Washing

++HOCLHOCLother chemicalWashing

SterilizationSterilizationWashing

SterilizationSterilization

CoolingSterilizationSterilization

Rinsing Cooling

FinishFinish

Rinsing

Finish微酸性電解水研究所 HOCL inc.

Predominance point pof deviceof device


⑨－１装置の特徴

Double module system

PP P PP

P PP

HOCLHOCL OthersHOCLHOCL Ot e s


Double busts oblong structure g

HOCL water+HOCL+HOCL Others

HOCL water

Rawmaterialmaterial

RawRaw

materialRawwater

Raw water SAEWSAEW


ApplicationApplication&&

Project Reference


Documents

slightly acidic electrolyzed water 4Oct16€¦ · The amount of fuel and water consuming for sterilization after CIP were calculated. The sample line including pipe line, tank, pump