Smoke Analysis Sub-Group (Routine Analytical Chemistry Sub

Smoke Analysis Sub-Group (Routine Analytical Chemistry Sub-Group)

Technical Report

2020 Collaborative Study of CORESTA Monitor 9 (CM9)

for the Determination of Test Piece

Weight, TPM, Water, Nicotine, NFDPM, Carbon Monoxide and Puff Count Obtained under Mainstream

‘Non-Intense’ and ‘Intense’

Smoking Regimes

August 2021

Coordinator:

Thomas Schmidt, Borgwaldt, Germany

Author:

Michael J. Morton, Altria Client Services, USA

Table of Contents

1. Introduction ....................................................................................................................... 3

2. Organisation ...................................................................................................................... 3

2.1 Participants .............................................................................................................. 3

2.2 Protocol ................................................................................................................... 4

3. Raw Data ........................................................................................................................... 5

4. Statistical Analysis ............................................................................................................ 6

4.1 Repeatability and Reproducibility Estimation ......................................................... 7

4.2 Z-Scores ................................................................................................................... 8

5. Comparison to Prior Collaborative Studies .................................................................... 10

6. Conclusion ...................................................................................................................... 12

APPENDIX A – Experimental Protocol .................................................................................. 13

APPENDIX B – Complete Data Set ........................................................................................ 25

APPENDIX C – Graphs of Results .......................................................................................... 32

APPENDIX D – Graphs of Z-Scores ....................................................................................... 39

RAC-270-CTR CM9 Coll. Study 2020 – August 2021 3/42

1. Introduction

The CORESTA Routine Analytical Chemistry Sub-Group (RAC SG) was given the

responsibility to organize the annual testing of the CORESTA Monitor test piece (tp) to verify

its continued suitability as a monitor. The responsibility is now under the Smoke Analysis

Sub-Group (SA SG) following the merger of the RAC SG with the Smoke Analytes Sub-Group

(SMA SG) in 2020.

The current monitor is CORESTA Monitor 9 (CM9).

The 2020 study was designed:

• to measure mainstream non-intense (ISO 3308) and intense (ISO 20778) smoke yields

of nicotine-free dry particulate matter (NFDPM or tar), nicotine, and carbon monoxide

(CO) for CM9

• to determine intra- and inter-laboratory variability for the measured non-intense and

intense smoke yields for CM9

• to verify the conditioned weight of CM9

2. Organisation

2.1 Participants

In total 25 laboratories participated in the 2020 study and are listed in alphabetical order in

Table 1. The laboratories were coded, and the codes shown in this report do not match the list

order given in the table.

Table 1: Participating Laboratories Listed in Alphabetical Order

Company Country Company Country

ASL Analytic Service Laboratory GmbH

Germany Manifatture Sigaro Toscano S.p.A. Italy

British American Tobacco Indonesia Philip Morris International Brazil

British American Tobacco Brazil Philip Morris International Czech

Republic

C.I.T.M.P.S.A. Uruguay Philip Morris International Korea

Imperial, Kyiv (ITPU) Ukraine Philip Morris International Mexico

Inciensa Costa Rica Philip Morris International Poland

Japan Tobacco Inc. Japan Philip Morris International Russia

JT International GmbH Germany Philip Morris International Turkey

Karelia Greece PT HM Sampoerna Indonesia

KT&G South Korea R.J. Reynolds Tobacco Company USA

Labstat Canada Tabacalera del Este S.A. Paraguay

Landewyck Tobacco s.a. Luxembourg Tobacco Institute of Japan Japan

Liggett Group USA

All 25 participating laboratories delivered data for ISO 3308 for the CM9 test piece using a

total of 34 smoking machines (22 rotary smoking machines and 12 linear smoking machines).

13 laboratories delivered data for ISO 20778 for the CM9 test piece using 15 smoking machines

(10 linear smoking machines and 5 rotary smoking machines).


Table 2 summarises the number and types of smoking machines used.

Table 2: Number of smoking machines

Machine Class ISO 3308 ISO 20778

Linear smoking machines 12 10

SM410-CV 1 1

SM450 11 9

Rotary smoking machines 22 5

RM200 2 0

RM200A 12 3

RM200A2 1 1

RM20D 2 0

RM20H 4 1

Unspecified 1 0

Total 34 15

2.2 Protocol

Participants were requested to follow the protocol “Annual Study of the CORESTA Monitor

Test Piece CM9 - 2020 Experimental Protocol” (provided in Appendix A) to analyse the

product CM9 and to report the seven parameters listed in Table 3.

Table 3: Parameters to be reported

Parameter Number of replicates Unit Code

Conditioned Weight 5 mg/tp Weight

Total Particulate Matter 5 mg/tp TPM

Water content (smoke) 5 mg/tp Water

Smoke Nicotine 5 mg/tp Nicotine

Nicotine Free Dry Particulate Matter 5 mg/tp NFDPM or Tar

Carbon Monoxide 5 mg/tp CO

Puff count 5 puff/tp Puff Ct

Replicates under ISO 3308 consisted of 4 ports each smoked with 5 tp per port on linear

machines or a single set of 20 tp on rotary machines for a total of 20 tp per port for both linear

and rotary machines. Replicates under ISO 20778 consisted of 4 ports with 3 tp per port for

linear smoking machines or a single set of 10 tp on rotary machines for a total of 12 tp for linear

machines and 10 tp for rotary machines. Replicates for weight consist of 20 tp. CM9 tp are

smoked with a fixed butt length of 33 mm for both smoking regimes and the smoking

parameters listed in the following table:


Table 4: Mainstream ISO and Intense smoking regime parameters

Smoking Regime

Puff Volume (mL)

Puff Frequency (s)

Puff Duration (s)

Vent. Blocking (%)

ISO 3308 35 60 2 0

ISO 20778 55 30 2 100

The participants bought the CM9 test piece individually via Borgwaldt KC or Cerulean.

3. Raw Data

Table 5 provides the averaged results for each laboratory. The complete data set is listed in

Appendix B and graphs of the data are shown in Appendix C.

Table 5: CM9 Average values by Lab

Lab Code

Type Weight

(g) TPM

(mg/tp) Water

(mg/tp) Nicotine (mg/tp)

NFDPM (mg/tp)

CO (mg/tp)

Puffs per tp

ISO 3308

1 Linear 941,2 17,31 1,63 1,466 14,21 NA 7,64

2 Linear 928,9 17,13 1,59 1,535 14,00 12,81 7,94

3 Linear 937,0 17,36 1,63 1,488 14,24 12,43 7,59

4 Rotary 936,0 17,13 2,05 1,533 13,55 13,26 7,45

5 Rotary 943,3 16,98 2,09 1,455 13,42 13,48 7,56

6A Rotary 946,3 16,51 1,89 1,420 13,20 13,63 7,37

6B Rotary 947,8 16,49 1,85 1,430 13,21 13,79 7,43

7 Linear 945,8 16,30 1,17 1,409 13,72 11,65 7,71

8A Linear 937,9 17,58 1,48 1,491 14,61 12,98 7,75

8B Rotary 939,8 16,42 1,89 1,415 13,12 13,77 7,34

9 Rotary 945,7 16,86 2,02 1,482 13,36 13,53 7,47

10 Rotary 943,2 16,98 1,77 1,454 13,75 13,74 7,61

11 Rotary 948,6 16,88 1,74 1,433 13,71 13,44 7,58

12 Linear 935,5 16,15 1,48 1,363 13,31 11,81 8,12

13 Rotary 950,3 17,39 1,99 1,431 13,97 13,88 7,68

14 Linear 940,2 17,25 1,41 1,534 14,31 12,43 7,90

15A Linear 941,1 16,74 1,62 1,440 13,69 12,70 7,88

15B Rotary 949,5 17,12 1,60 1,464 14,06 12,69 7,80

16 Rotary 942,5 17,09 1,99 1,385 13,71 NA 7,62

17A Rotary 947,4 16,83 1,83 1,446 13,56 13,59 8,52

17B Rotary 949,0 16,86 1,83 1,441 13,59 13,53 8,54

18A Rotary 939,9 16,88 1,79 1,455 13,64 13,44 7,48

18B Rotary 939,3 17,09 1,80 1,457 13,84 13,44 7,56

19 Linear 949,3 17,42 1,47 1,426 14,52 12,99 8,05


Lab Code

Type Weight

(g) TPM

(mg/tp) Water

(mg/tp) Nicotine (mg/tp)

NFDPM (mg/tp)

CO (mg/tp)

Puffs per tp

20A Rotary 942,0 16,99 1,95 1,396 13,64 13,24 7,63

20B Rotary 941,4 17,16 2,01 1,410 13,74 13,42 7,49

21A Rotary 946,2 16,58 1,74 1,400 13,44 12,22 7,56

21B Rotary 944,5 16,64 1,72 1,416 13,50 13,16 7,62

22A Linear 938,0 16,58 1,63 1,488 13,47 12,60 7,57

22B Rotary 931,0 15,90 1,90 1,477 12,51 12,32 7,21

23A Linear 943,8 17,25 1,30 1,421 14,51 13,49 7,89

23B Rotary 944,2 16,98 1,91 1,396 13,68 13,54 7,44

24 Rotary 945,2 17,12 2,23 1,442 13,44 13,68 7,50

25 Linear 931,4 17,66 1,64 1,547 14,47 12,36 8,06

ISO 20778

2 Linear NA 44,25 11,47 3,237 29,55 23,93 10,97

3 Linear NA 51,02 12,87 3,223 34,92 23,49 10,68

4 Rotary NA 41,90 9,72 3,246 28,97 25,07 10,78

7 Linear NA 43,65 10,21 3,088 30,35 23,65 10,73

8A Linear NA 45,15 12,14 3,153 29,85 24,34 10,70

8B Rotary NA 38,37 8,80 2,886 26,68 24,62 10,45

9 Rotary NA 41,08 9,33 3,092 28,66 24,74 10,73

13 Rotary NA 41,63 9,87 3,007 28,76 25,07 10,91

14 Linear NA 46,01 12,55 3,201 30,26 23,83 10,96

15A Linear NA 46,14 10,55 3,187 32,40 25,04 10,97

19 Linear NA 44,95 10,41 2,833 31,71 24,30 11,36

22A Linear NA 45,43 11,60 3,197 30,63 24,59 10,82

23A Linear NA 45,37 11,40 3,028 30,94 25,08 11,05

23B Rotary NA 41,11 8,94 2,982 29,19 25,24 10,73

25 Linear NA 47,25 12,56 3,208 31,49 23,82 11,31

4. Statistical Analysis

The statistical evaluation of data for this collaborative study followed the methods provided by

ISO 5725-5[1]. This procedure does not involve testing for outliers, but, instead, employs robust

procedures which are little influenced by the presence of outliers. Nonetheless, CO from

Lab 12 rep 4 was dropped as a single-point outlier.

Z-scores were also calculated to assess laboratory performance and to aid laboratory

accreditation. The test was performed with both linear and rotary smoking machines together.

[1] “ISO 5725-5:1998: Accuracy (trueness and precision) of measurement methods and results – Part 5: Alternative

method for the determination of the precision of a standard measurement method.


Z-scores were calculated in basic conformance with ISO 13528 and used Algorithm A to

estimate the assigned values and standard deviations for proficiency assessment.

4.1 Repeatability and Reproducibility Estimation

Repeatability and reproducibility limits are calculated for both smoking regimes for each

parameter. The results are summarized in Tables 6-9, with all machines grouped together and,

where applicable, with linear or rotary machines separately. For ISO 20778, only linear

machines are shown separately, since so few laboratories reported results under ISO 20778 with

rotary machines. The number of laboratories provided in the tables correspond to the number

of laboratories from which data were provided.

Table 6: r&R estimations for CM9 under ISO 3308 smoking regime (all machines)

Parameter Mean N Labs r r (%) R R (%)

Weight (mg/tp) 942,5 34 9,74 1,03 % 17,48 1,85 %

TPM (mg/tp) 16,9 34 0,77 4,52 % 1,30 7,66 %

Water (mg/tp) 1,76 34 0,34 19,55 % 0,73 41,7 %

Nicotine (mg/tp) 1,45 34 0,06 4,44 % 0,13 9,19 %

NFDPM (mg/tp) 13,7 34 0,60 4,36 % 1,34 9,79 %

CO (mg/tp) 13,1 32 0,69 5,23 % 1,84 14,05 %

Puffs per tp 7,66 34 0,30 3,88 % 0,76 9,95 %

Table 7: r&R estimations for CM9 under ISO 3308 smoking regime (machines separated)

Parameter Machine

Type Mean N Labs r r (%) R R (%)

Weight (mg/tp) Linear 939,2 12 10,48 1,12 % 19,68 2,10 %

Weight (mg/tp) Rotary 944,1 22 9,30 0,98 % 14,77 1,56 %

TPM (mg/tp) Linear 17,1 12 1,07 6,28 % 1,80 10,55 %

TPM (mg/tp) Rotary 16,9 22 0,54 3,22 % 0,95 5,65 %

Water (mg/tp) Linear 1,51 12 0,32 20,96 % 0,50 32,80 %

Water (mg/tp) Rotary 1,89 22 0,35 18,72 % 0,51 26,79 %

Nicotine (mg/tp) Linear 1,47 12 0,09 5,92 % 0,19 13,02 %

Nicotine (mg/tp) Rotary 1,44 22 0,06 3,91 % 0,10 7,24 %

NFDPM (mg/tp) Linear 14,1 12 0,90 6,36 % 1,60 11,37 %

NFDPM (mg/tp) Rotary 13,6 22 0,46 3,38 % 0,88 6,48 %

CO (mg/tp) Linear 12,6 11 1,03 8,17 % 1,85 14,72 %

CO (mg/tp) Rotary 13,5 21 0,52 3,86 % 0,95 7,03 %

Puffs per tp Linear 7,8 12 0,36 4,57 % 0,68 8,63 %

Puffs per tp Rotary 7,5 22 0,27 3,53 % 0,48 6,41 %


Table 8: r&R estimations for CM9 under ISO Intense 20778 smoking regime (all machines)

Parameter Mean N Labs r r (%) R R (%)

TPM (mg/tp) 44,1 15 2,19 4,96 % 8,23 18,64 %

Water (mg/tp) 10,8 15 1,32 12,20 % 4,48 41,3 %

Nicotine (mg/tp) 3,11 15 0,12 3,79 % 0,39 12,47 %

NFDPM (mg/tp) 30,2 15 1,40 4,62 % 4,74 15,70 %

CO (mg/tp) 24,5 15 1,24 5,09 % 2,19 8,95 %

Puffs per tp 10,9 15 0,40 3,71 % 0,70 6,46 %

Table 9: r&R estimations for CM9 under ISO Intense 20778 smoking regime (linear machines)#

Parameter Machine

Type Mean N Labs r r (%) R R (%)

TPM (mg/tp) Linear 45,6 10 2,68 5,87 % 4,62 10,13 %

Water (mg/tp) Linear 11,6 10 1,38 11,93 % 3,28 28,30 %

Nicotine (mg/tp) Linear 3,15 10 0,12 3,74 % 0,28 8,79 %

NFDPM (mg/tp) Linear 31,0 10 1,91 6,15 % 3,86 12,45 %

CO (mg/tp) Linear 24,2 10 1,34 5,53 % 2,15 8,86 %

Puffs per tp Linear 11,0 10 0,45 4,11 % 0,85 7,80 %

# Rotary machines were not broken out separately because there were too few labs reporting data under ISO 20778.

4.2 Z-Scores

Z-scores are not, strictly speaking, an aspect of the CM9 evaluation, but, instead, were provided

as a courtesy to the laboratories to assist in their accreditation. The values were calculated in

general conformance with ISO 13528. Z-scores are given in standard deviation units deviation

from the assigned value (“average”), and the assigned values and standard deviations for

proficiency assessment were estimated using Algorithm A. It is expected that most of the

z-scores should fall within the range of ±2. Values such that 2≤|z|<3 should be interpreted as a

warning, and that laboratories having values with |z|>3 should be treated as an “action signal”

to investigate laboratory performance. The z-scores are given in Tables 10 and 11 for ISO 3308

and ISO 20778, respectively. Generally, the labs performed similarly and the only two z-scores

that exceeded 3 in magnitude were puff count under ISO 3308 for Lab 17A and 17B.

Table 10: Z-scores for each laboratory under ISO 3308

ISO 3308

Lab Code Weight TPM Water Nicotine NFDPM CO Puff Ct

Avg# 942.5 16.95 1.760 1.446 13.73 13.12 7.66

StDev# 5.41 0.39 0.238 0.043 0.44 0.62 0.26

1 -0,24 0,92 -0,54 0,46 1,10 NA -0,09

2 -2,52 0,46 -0,72 2,09 0,63 -0,51 1,08

3 -1,02 1,06 -0,55 0,98 1,18 -1,12 -0,29

4 -1,20 0,48 1,20 2,04 -0,39 0,22 -0,85


ISO 3308

Lab Code Weight TPM Water Nicotine NFDPM CO Puff Ct

5 0,14 0,09 1,40 0,22 -0,69 0,58 -0,41

6A 0,70 -1,11 0,55 -0,60 -1,20 0,82 -1,15

6B 0,99 -1,16 0,39 -0,38 -1,18 1,08 -0,93

7 0,60 -1,64 -2,47 -0,85 -0,01 -2,37 0,19

8A -0,85 1,60 -1,20 1,07 2,01 -0,23 0,35

8B -0,51 -1,34 0,54 -0,71 -1,38 1,05 -1,28

9 0,59 -0,22 1,08 0,84 -0,84 0,65 -0,75

10 0,12 0,08 0,04 0,20 0,06 0,99 -0,22

11 1,13 -0,17 -0,10 -0,30 -0,03 0,50 -0,33

12 -1,29 -2,01 -1,17 -1,93 -0,95 -2,12 1,79

13 1,45 1,12 0,95 -0,34 0,55 1,22 0,08

14 -0,42 0,77 -1,48 2,06 1,32 -1,13 0,93

15A -0,25 -0,52 -0,61 -0,13 -0,09 -0,68 0,85

15B 1,30 0,45 -0,69 0,42 0,76 -0,69 0,55

16 0,00 0,36 0,96 -1,41 -0,03 NA -0,18

17A 0,91 -0,29 0,29 0,00 -0,39 0,75 3,37

17B 1,19 -0,22 0,31 -0,11 -0,32 0,65 3,43

18A -0,49 -0,16 0,13 0,21 -0,20 0,50 -0,70

18B -0,60 0,37 0,16 0,26 0,25 0,51 -0,42

19 1,26 1,19 -1,21 -0,47 1,80 -0,22 1,50

20A -0,09 0,12 0,81 -1,16 -0,19 0,18 -0,14

20B -0,20 0,54 1,04 -0,83 0,03 0,48 -0,67

21A 0,68 -0,93 -0,09 -1,07 -0,65 -1,46 -0,41

21B 0,38 -0,78 -0,17 -0,69 -0,51 0,06 -0,17

22A -0,83 -0,93 -0,57 0,98 -0,59 -0,84 -0,39

22B -2,13 -2,67 0,60 0,74 -2,75 -1,29 -1,78

23A 0,24 0,77 -1,94 -0,57 1,77 0,59 0,87

23B 0,31 0,09 0,63 -1,16 -0,11 0,67 -0,88

24 0,50 0,44 1,99 -0,09 -0,64 0,89 -0,63

25 -2,05 1,81 -0,49 2,36 1,69 -1,23 1,54

# The listed Avgs and StDevs are the averages and standard deviations used in the calculation of the z-scores.


Table 11: Z-scores for each laboratory reporting under ISO Intense 20778

ISO 20778 (Intense)

Lab Code TPM Water Nicotine NFDPM CO Puff Ct

Avg# 44.15 10.83 3.112 30.21 24.46 10.86

StDev# 2.85 1.54 0.133 1.63 0.67 0.21

2 0,04 0,42 0,94 -0,41 -0,79 0,50

3 2,41 1,32 0,83 2,88 -1,44 -0,85

4 -0,79 -0,72 1,01 -0,76 0,91 -0,40

7 -0,17 -0,40 -0,18 0,08 -1,19 -0,63

8A 0,35 0,85 0,31 -0,22 -0,17 -0,74

8B -2,03 -1,32 -1,69 -2,16 0,24 -1,90

9 -1,07 -0,97 -0,15 -0,95 0,42 -0,63

13 -0,88 -0,62 -0,79 -0,89 0,92 0,21

14 0,65 1,12 0,67 0,03 -0,93 0,45

15A 0,70 -0,18 0,57 1,34 0,87 0,49

19 0,28 -0,27 -2,09 0,92 -0,23 2,29

22A 0,45 0,50 0,64 0,26 0,21 -0,18

23A 0,43 0,37 -0,62 0,45 0,94 0,89

23B -1,07 -1,22 -0,97 -0,63 1,17 -0,61

25 1,09 1,12 0,72 0,78 -0,94 2,10

# The listed Avgs and StDevs are the averages and standard deviations used in the calculation of the z-scores.

5. Comparison to Prior Collaborative Studies

This section compares the results of this study with previous collaborative studies of different

test pieces conducted by the Routine Analytical Chemistry Sub-Group or by the Smoke

Analysis Sub-Group under the ISO 3308 and ISO 20778 smoking regimes and for test piece

weight. Keep in mind that the number of smoking machines, smoking machine types and

participants varies throughout the studies.

Tables 12-14 list the averages, repeatability limits, and reproducibility limits for the test pieces.


Table 12: Averages, repeatability, reproducibility of CORESTA collaborative studies from 2011 to 2020 for different test pieces under ISO 3308

smoking conditions

ISO 3308 Year 2011 2012 2013 2014 2014 2016 2017 2018 2018 2019 2020

Test piece CM7 CM7 CM7 CM7 CM8 CM8 CM8 CM8 CM9 CM9 CM9

Avera

ge (

mg

/tp

or

pu

ffs p

er

tp)

TPM 16,37 16,28 16,16 16,15 17,57 17,33 17,37 17,24 16,98 16,98 16,95

Water 1,78 1,75 1,71 1,71 1,84 1,81 1,82 1,79 1,68 1,87 1,76

Nicotine 1,236 1,221 1,221 1,204 1,650 1,657 1,672 1,650 1,48 1,443 1,446

NFDPM 13,34 13,29 13,23 13,25 14,06 13,86 13,95 13,81 13,82 13,71 13,73

CO 12,93 12,84 12,76 12,83 13,42 13,24 13,21 13,12 13,06 13,32 13,12

Puff 8,36 8,36 8,32 8,23 7,70 7,72 7,78 7,88 7,76 7,55 7,66

Rep

ea

tab

ilit

y (

r) TPM 0,93 0,80 0,85 0,87 1,06 1,05 1,07 1,03 0,96 0,90 0,767

Water 0,41 0,41 0,39 0,36 0,39 0,35 0,40 0,43 0,45 0,32 0,344

Nicotine 0,075 0,066 0,076 0,075 0,119 0,093 0,096 0,090 0,095 0,094 0,064

NFDPM 0,77 0,78 0,72 0,72 0,82 0,89 0,89 0,90 0,80 0,80 0,598

CO 0,95 0,78 0,75 0,79 1,05 0,79 0,97 0,97 1,03 0,97 0,687

Puff 0,38 0,34 0,31 0,36 0,30 0,39 0,36 0,36 0,35 0,26 0,298

Rep

rod

ucib

ilit

y (

R) TPM 1,77 1,67 1,61 1,93 1,89 1,95 1,93 1,92 1,56 1,36 1,298

Water 0,97 1,03 0,98 1,01 1,12 1,08 1,15 0,95 0,91 0,86 0,733

Nicotine 0,150 0,143 0,146 0,155 0,197 0,202 0,254 0,17 0,185 0,214 0,133

NFDPM 1,88 1,73 1,85 2,16 1,97 1,91 1,86 1,69 1,42 1,46 1,343

CO 1,94 1,75 1,61 1,97 2,02 1,52 1,97 1,64 1,79 1,95 1,844

Puff 0,86 0,93 0,91 0,82 0,75 0,83 1,00 0,87 0,94 0,61 0,763


Table 13: Averages, repeatability, reproducibility of CORESTA collaborative studies from 2013

to 2020 for different test pieces under ISO intense 20778 smoking conditions

ISO Intense 20778 smoking regime

Year 2013 2016 2017 2018 2018 2019 2020

Test piece CM7 CM8 CM8 CM8 CM9 CM9 CM9

Average (mg/tp or

puffs per tp)

TPM 41,56 43,08 43,36 42,83 42,84 42,60 44,15

Water 10,33 10,01 10,29 10,27 10,19 9,79 10,83

Nicotine 2,493 3,400 3,425 3,373 3,107 3,079 3,112

NFDPM 28,39 29,65 29,65 29,19 29,44 29,25 30,21

CO 24,72 24,88 24,86 24,89 24,71 24,65 24,46

Puff 11,79 11,08 11,12 11,28 10,97 10,87 10,86

Repeatability (r)

TPM 2,38 1,96 2,17 2,17 1,79 1,63 2,19

Water 1,23 0,98 1,33 1,18 1,28 1,17 1,32

Nicotine 0,152 0,152 0,161 0,199 0,179 0,126 0,118

NFDPM 1,57 1,39 1,30 1,64 1,62 1,55 1,40

CO 1,30 1,04 1,24 1,31 1,25 1,02 1,24

Puff 0,52 0,47 0,43 0,51 0,50 0,42 0,40

Reproducibility (R)

TPM 10,53 9,47 7,86 8,72 8,93 7,37 8,23

Water 4,77 3,79 4,18 4,86 3,71 4,33 4,48

Nicotine 0,313 0,508 0,416 0,405 0,275 0,235 0,388

NFDPM 5,66 5,03 4,56 4,47 4,72 4,32 4,74

CO 3,01 2,00 2,72 2,83 2,46 2,11 2,19

Puff 1,27 0,92 1,18 1,12 1,10 0,84 0,70

Table 14: Average weight data, repeatability and reproducibility for test pieces CM9

Study CM9 2018 CM9 2019 CM9 2020

Weight (mg/tp) 943,2 941,49 942,5

Repeatability (r) 13,3 9,53 9,74

Reproducibility (R) 17,9 15,83 17,48

The averages and the estimated variabilities are generally comparable to those seen in prior

years. This supports the continued suitability of the CM9 for use as a laboratory monitor.

6. Conclusion

This study has examined the performance of CM9 under the ISO 3308 and ISO 20778 smoking

regimes to determine if CM9 continues to be suitable as a monitor test piece. The performance

of the monitor in this testing was quite similar to its historical performance and continues to be

a suitable smoke analysis monitor.


APPENDIX A – Experimental Protocol

Routine Analytical Chemistry Sub-Group

Collaborative Study

ANNUAL STUDY OF THE CORESTA

MONITOR TEST PIECE CM9 2020

EXPERIMENTAL PROTOCOL

Thomas Schmidt

Borgwaldt KC GmbH,

GERMANY

12 June 2020


Foreword

This CORESTA protocol describes the elements that the Standards Task Force has identified

as important for describing elements of an experimental protocol for a collaborative study or a

proficiency study as defined below (Horwitz 862):

• Collaborative study

“A collaborative study is an interlaboratory study in which each laboratory uses the defined

method of analysis to analyse identical portions of homogeneous materials to assess the

performance characteristics obtained for that method of analysis.”

CORESTA recognizes that collaborative studies require considerable effort and should be

conducted only on methods that have received adequate prior testing.

• Proficiency Study

“A proficiency study is an interlaboratory study consisting of one or more assays- conducted

by a group of laboratories on one or more identical materials, by whatever method is in use in

each laboratory, for the purpose of comparing the results of each laboratory with those of other

laboratories, with the objective of evaluating or improving performance.”

Proficiency studies often serve as pre-work for selecting a method for a full collaborative study.


Table of Contents

Foreword .................................................................................................................................... 2

1. Introduction ................................................................................................................... 4

2. Objectives ...................................................................................................................... 4

3. Study Coordinator(s) ..................................................................................................... 4

4. Normative References ................................................................................................... 4

5. Methods ......................................................................................................................... 5

6. Target Variables and Data Reporting ............................................................................ 8

7. Study Time Table ........................................................................................................ 10

8. Data Submission .......................................................................................................... 10

9. Explanation of Minimum Statistics to be Applied for a Collaborative Study ............. 10

Bibliography ........................................................................................................................... 11

Appendix A – Système International (SI) Units and their Symbols ....................................... 12


1. Introduction

The CORESTA Routine Analytical Chemistry Sub Group has been given the responsibility to

organize the annual testing of the CORESTA Monitor test piece. This year the study will

include CM9.

2. Objectives

This year’s study will have expanded objectives that include smoking the monitor test piece

under both ISO and a specified intense smoking regime. The study objectives are:

• To measure mainstream smoke yields of nicotine-free dry particulate matter

(NFDPM), nicotine and carbon monoxide (CO) when smoked under ISO and a

specified intense smoking regime

• To establish the conditioned weight of CM8 prior to smoking

• To determine intra- and inter-laboratory variability for the measured smoke yields and

to continue this comparison over time

• To obtain statistical information about possible differences in smoke yields from

different smoking machines

3. Study Coordinator(s)

Thomas Schmidt

4. Normative References

The relevant Standards for ISO, Intense smoking and statistical analyses are:

Definitions and standard conditions ISO 3308

Conditioning ISO 3402

TPM / NFDPM ISO 4387

Carbon monoxide ISO 8454

Nicotine ISO 10315

Water (GC) ISO 10362-1

Statistical Analysis ISO 5725-2

Ventilation zone blocking, puffing parameters, and number of test pieces smoked

*Health Canada Method T-115 paragraph 16.1

Tar and Nicotine and

CO T-115e4.pdf

*Note: T-115 has been removed from the Health Canada website and therefore is embedded here


5. Methods

5.1 General Guidance

As a general guide, experiments conducted under each puffing regime should be conducted

according to ISO standard requirements. Exceptions to this guiding principle include the

puffing parameters applied for intense smoking regime, the vent blocking applied, the number

of cigarettes smoked per sample and the butt length for smoking. The study (the smoking

results) and the daily use of the monitor are based on a fixed butt length of 33 mm forCM9.

Please note that any operating conditions (a) not specified in the above ISO standards, or

regarded as optional, (b) not specified in the intense method documents provided as guidelines

and (c) any circumstances that may have influenced test results should be noted in the test

report.

Puffing parameter and vent blocking specifications for each puffing regime are summarized in

Table 1. Note: Although the CORESTA Monitor Test Pieces are unventilated it is advised to

follow the Health Canada procedures exactly and tape the filter ventilation zone.

Table 1: Smoking Regime Specifications

Smoking Regime

Puff Volume

(mL)

Puff Frequency*

(s)

Puff Duration

(s)

Vent Blocking

(%) Description

1 35 ± 0.3 60 ± 0.5 2 ± 0.02 0 ISO

2 55 ±0.5 30 ± 1.0 2 ± 0.02 100 Intense

5.2. Test Specimen(s)

CM9 test piece.

5.3. Sampling

Each participating laboratory is responsible for procuring their own supply of CM9 through the

normal channels (Borgwaldt KC or Cerulean). The smoking plans will use 200 test pieces for

each rotary smoking machine or 220 test pieces for each linear smoking machine included in

the study. A composite of all packs should be prepared for conditioning according to ISO

8243:2006. If a substantial period of time is expected to occur between the times when the test

pieces are smoked with the different smoking regimes, then two separate composite samples

should be prepared, one for each smoking regime.

5.4. Ventilation Zone Blocking for Intense Smoking

The blocking of the filter ventilation zone will be achieved by wrapping a single layer of

invisible tape, 19.0 mm wide or equivalent, around the complete circumference of the filter and

tipping paper of the test articles.

5.5. Butt Marking

For the purpose of this study the CM9 test pieces will be butt marked to a fixed butt length of

33 mm.


5.6. Conditioning

The composite samples shall be conditioned for at least 48 hours but no more than 10 days

under the following conditions:

Temperature: 22 ± 1 °C Relative humidity: 60 ± 3 %

Temperature and relative humidity of the conditioning enclosure (chamber or room) shall be

checked and recorded for every smoking run.

Test cigarettes to be smoked for the determination of NFDPM, nicotine and CO should be

selected at random from the conditioned cigarettes.

Any test pieces with visible defects shall be discarded.

5.7. Test Piece Weight

The test articles will be weighed for ISO smoking only since the ventilation zone blocking tape

applied for intense smoking may vary between laboratories. The weight of the test piece after

conditioning is to be determined by weighing all test pieces to be smoked just before they are

placed at the smoking machine. The result is reported as the mean weight of the 20 test pieces

for linear or rotary smoking machine at ISO puffing parameters.

5.8. Smoking Machine

Where smoking laboratories have more than one type of smoking machine (i.e. linear and rotary

variants) they are encouraged to include as many types in the study as possible.

5.9. Smoking Plans

The smoking plans will incorporate one product (CM9), shown as M in the smoking plans.

These plans are designed to smoke the CM9 in all ports for each puffing regime, spread over a

number of days. If possible, each participating laboratory should use only one operator (for

each smoking machine type) throughout the course of the study.

For ISO smoking, one test result is defined as the mean yield obtained from smoking 20 test

pieces in a single smoking machine run, therefore from a rotary machine it is the result from

one run smoking 20 test pieces and from a linear machine it is the average of four ports, smoking

five test pieces per port. This is repeated on five separate days resulting in 100 test pieces being

smoked (Tables 2-3).

For Intense smoking, the number of test pieces smoked per filter pad is reduced to avoid

overloading the pad. As a consequence, the number of smoking runs/ports has to be increased

so that the same nominal numbers of test pieces are smoked. Thus, a test result from a rotary

machine is the mean of two runs, each of 10 test pieces, and from a linear machine it is the

average of eight ports, smoking three test pieces per port (Tables 4-5).

Note that the side seam of the test pieces should be positioned at random and not in a fixed

position since it has been observed that some laboratory staff habitually place cigarettes in a

particular orientation. Ports not occupied by the test pieces are left blank, which may be

assigned to any other cigarette that is to be smoked by the laboratory, or the ports may be left

empty.


Table 2: Smoking Plan for 20-port Linear Machines for ISO smoking (5 test pieces per pad) D

ay

Port number

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

1 M M M M

2 M M M M

3 M M M M

4 M M M M

5 M M M M

Table 3: Smoking Plan for 20-port Rotary Machines for ISO smoking (20 test pieces per pad)

Day Run Test Piece

1 1 M

2 2 M

3 3 M

4 4 M

5 5 M

Table 4: Smoking Plan for 20-port Linear Machines for Intense smoking (3 test pieces per pad)

Day

Ru

n

Port number

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

1 6 M M M M

7 M M M M

2 8 M M M M

9 M M M M

3 10 M M M M

11 M M M M

4 12 M M M M

13 M M M M

5 14 M M M M

15 M M M M


Table 5: Smoking Plan for 20-port Rotary Machines for Intense smoking (10 test pieces per pad)

Day Run Test Piece

1 6 M

7 M

2 8 M

9 M

3 10 M

11 M

4 12 M

13 M

5 14 M

15 M

6. Target Variables and Data Reporting

The variables to be reported fall into two categories: those which will form the basis of the

statistical evaluation of the data and those that are ancillary to the test.

The variables to be reported for statistical analysis are:

• Mean weight of the Monitor after conditioning (just prior to smoking)

• Mean puff number per test piece

• Mean TPM per test piece

• Mean nicotine delivery per test piece

• Mean water delivery per test piece

• Mean NFDPM delivery per test piece

• Mean CO delivery per test piece

The ancillary variables are:

• Date of test

• Type of smoking machine used

• Temperature (C°) and relative humidity (RH%) of conditioning enclosure

• Temperature (C°) and relative humidity (RH%) of smoking environment

• Atmospheric pressure in the laboratory during smoking

• Ambient air flow in the smoking machine before smoking (mm/sec)


Dimensions and rounding of test results

Analytical variables:

Weight of Monitor mg per test piece ####.#

Puff number number ##.##

TPM mg per test piece ##.##

Nicotine mg per test piece #.###

Water mg per test piece ##.##

NFDPM mg per test piece ##.##

Carbon Monoxide mg per test piece ##.##

Ancillary variables:

Date of test DD/MM/YY

Conditioning temperature degrees Celsius ##.#

Conditioning humidity percent RH ##.#

Air velocity mm/s ###

Laboratory temperature degrees Celsius ##.#

Laboratory humidity percent RH ##.#

Laboratory barometric pressure kPa ###.#

Please note that the rounding of the data to the formats specified above must take place

after any calculations that may be involved. All calculations will use the laboratory data

as recorded using the maximum number of digits available.

All data arising from this study will be made available to the laboratories participating in the

study. The distribution of data will be done after collation and statistical evaluation.

To facilitate the statistical evaluation, all data must be reported using the attached spreadsheets

and must be submitted via e-mail. Please use the attached spreadsheets as provided without

changing the format.


7. Study Time Table

The smoking should be performed during June and July 2020. Strict adherence to the timeline

is very important for the data to be compiled for an initial assessment at the next Sub-Group

meeting in October, 2020. Data not received by August 21, 2020 will be excluded from the

formal statistical analysis. Please review your data carefully before submitting as the statistical

analysis is very labor intensive.

The statistical analysis of the results will follow as closely as possible the recommendations of

ISO 5725.

Should any questions arise please contact the study coordinator, Thomas Schmidt. All results

must be sent via e-mail to:

Thomas Schmidt

8. Data Submission

All results must be sent via e-mail to:

Thomas Schmidt

9. Explanation of Minimum Statistics to be Applied for a Collaborative

Study

At a minimum Repeatability and Reproducibility estimation will be applied.


Bibliography

• Chem, Pure Appl. QUALITY ASSURANCE SCHEMES THE INTERNATIONAL

HARMONIZED PROTOCOL FOR THE PROFICIENCY TESTING OF ANALYTICAL

CHEMISTRY LABORATORIES (n.d.): n. pag. Web.

• Council of Science Editors. 8th ed. N.p.: Council of Science Editors, n.d. Print.

• Form and Style for ASTM Standards. West Conshohocken, PA: ASTM

INTERNATIONAL, 2015. Print.

• Horwitz, William. "Protocol for the Design, Conduct and Interpretation of Collaborative

Studies." Pure and Applied Chemistry 60.6 (1988): 855-64. ShareGate. International Union

of Pure and Applied Chemistry, De Gruyter, Jan. 1988. Web. 30 July 2015.

• ISO 5725 Standard And Reference Laboratories Error.pdf. "ISO 5252." ISO 5725 Standard

And Reference Laboratories Error (n.d.): n. pag. Web.

• "IUPAC 2006 IUPAC, Pure and Applied Chemistry 78, 145–196"" N.p., n.d. Web. 25 Aug.

2015.

• "Numbers, Units, Mathematicial Expressions, and Statistics." Scientific Style and Format;

The CSE Manual for Authors Editors, and Publishers. 8th ed. Chicago: The University of

Chicago, 2014. 157-91. Print.


Appendix A – Système International (SI) Units and their Symbols

SI base units

Base Quantity Name Symbol

Length meter m

Mass kilogram Kg

Time second s

Electric current ampere A

Temperature kelvin K

Amount of substance mole mol

Luminous intensity Candela cd

SI derived units

Derived Quantity Name Symbol Equivalent SI Units

Frequency hertz Hz s-1

Force newton N m·kg·s-2

Pressure pascal Pa N/m2

Energy joule J N·m

Power watt W J/s

Electric charge coulomb C s·A

Electric potential volt V W/A

Electric resistance ohm Ω V/A

Celsius temperature Degrees Celsius °C K*

*Unit degree Celsius is equal in magnitude to unit kelvin

SI prefixes

Factor Name Symbol Numerical value

1012 tera T 1 000 000 000 000

109 giga G 1 000 000 000

106 mega M 1 000 000

103 kilo k 1 000

102 hecto h 100

101 deka da 10

10-1 deci d 0.1

10-2 centi c 0.01

10-3 milli m 0.001

10-4 micro µ 0.000 001

10-6 nano n 0.000 000 001

10-7 pico p 0.000 000 000 001


APPENDIX B – Complete Data Set

Lab Code Type Weight mg/tp

TPM mg/tp

Water mg/tp

Nicotine mg/tp

NFDPM mg/tp

CO mg/tp

Puffs/tp

ISO 3308

1 Linear

943,5 17,50 1,61 1,498 14,39 – 7,80

934,9 16,70 1,46 1,393 13,84 – 7,33

943,3 17,68 1,74 1,503 14,44 – 7,75

940,0 17,54 1,68 1,478 14,38 – 7,70

944,2 17,13 1,67 1,458 14,00 – 7,63

2 Linear

929,5 17,19 1,59 1,559 14,04 12,93 8,00

927,3 17,16 1,53 1,526 14,11 12,78 7,88

928,0 17,30 1,68 1,540 14,07 12,80 7,95

926,2 17,14 1,58 1,544 14,02 12,61 7,93

933,3 16,86 1,57 1,506 13,78 12,92 7,95

3 Linear

941,4 17,22 1,64 1,466 14,12 12,20 7,63

935,1 17,57 1,63 1,493 14,45 12,35 7,53

938,0 17,29 1,74 1,461 14,08 12,56 7,70

934,6 16,77 1,45 1,490 13,83 12,05 7,55

935,8 17,97 1,70 1,529 14,74 12,99 7,55

4 Rotary

937,2 17,28 2,28 1,535 13,47 13,13 7,43

940,1 17,09 1,90 1,516 13,67 13,38 7,57

938,6 17,03 1,96 1,549 13,52 13,16 7,34

931,6 17,13 2,13 1,526 13,47 13,45 7,32

932,4 17,14 1,96 1,540 13,64 13,18 7,57

5 Rotary

947,8 16,87 2,13 1,487 13,25 13,54 7,56

943,7 17,06 2,03 1,460 13,56 13,45 7,58

942,4 17,09 2,14 1,459 13,48 13,71 7,48

939,5 16,99 2,06 1,430 13,49 13,43 7,65

943,0 16,89 2,11 1,439 13,34 13,29 7,53

6A Rotary

954,4 16,32 1,75 1,402 13,17 13,49 7,37

941,1 16,55 2,03 1,418 13,11 13,54 7,34

937,5 16,91 2,04 1,466 13,40 14,11 7,27

949,7 16,30 1,88 1,399 13,02 13,40 7,40

948,8 16,47 1,76 1,415 13,30 13,62 7,47

6B Rotary

952,8 16,47 2,04 1,421 13,01 13,94 7,55

951,1 16,49 1,89 1,410 13,19 13,72 7,47

945,1 16,56 1,75 1,444 13,36 13,78 7,38

941,4 16,47 1,72 1,432 13,32 13,69 7,28



TPM mg/tp

Water mg/tp

Nicotine mg/tp

NFDPM mg/tp

CO mg/tp

Puffs/tp

948,8 16,46 1,87 1,441 13,15 13,84 7,45

7 Linear

950,8 15,93 1,34 1,336 13,26 11,50 7,73

938,9 16,23 1,18 1,408 13,63 11,57 7,49

945,7 16,20 1,15 1,450 13,60 11,80 7,71

950,4 16,35 1,11 1,421 13,81 11,71 7,81

943,1 16,81 1,09 1,432 14,30 11,69 7,84

8A Linear

937,1 18,27 1,68 1,545 15,05 13,55 7,83

934,9 17,42 1,40 1,483 14,53 13,04 7,58

944,1 17,47 1,48 1,487 14,51 13,04 7,85

938,8 17,23 1,38 1,463 14,38 12,28 7,85

934,5 17,50 1,44 1,479 14,58 13,02 7,67

8B Rotary

938,0 16,30 1,87 1,397 13,04 13,43 7,31

944,6 16,39 1,88 1,427 13,09 13,71 7,48

933,9 16,45 1,86 1,408 13,18 13,90 7,18

940,0 16,61 1,94 1,430 13,24 14,00 7,33

942,3 16,34 1,89 1,414 13,04 13,83 7,39

9 Rotary

949,0 17,17 2,01 1,471 13,69 13,56 7,37

945,7 16,62 1,80 1,495 13,33 13,80 7,47

941,4 17,07 2,13 1,490 13,45 13,40 7,47

946,3 16,64 1,99 1,491 13,15 13,19 7,50

946,0 16,79 2,16 1,461 13,17 13,70 7,55

10 Rotary

947,6 17,01 1,95 1,479 13,58 13,91 7,72

948,8 17,15 2,03 1,474 13,65 14,08 7,67

941,3 17,13 1,72 1,433 13,98 13,58 7,60

941,3 17,06 1,75 1,464 13,84 13,75 7,66

936,8 16,54 1,40 1,421 13,72 13,39 7,39

11 Rotary

945,0 16,73 1,53 1,445 13,76 13,32 7,72

955,0 16,92 1,75 1,438 13,74 13,25 7,63

949,0 17,16 1,96 1,434 13,76 13,36 7,46

946,0 16,90 1,67 1,439 13,79 13,60 7,75

948,0 16,69 1,77 1,409 13,51 13,65 7,34

12 Linear

937,6 15,67 1,12 1,333 13,22 12,02 8,08

934,7 15,85 1,39 1,335 13,13 11,69 8,10

939,8 16,12 1,43 1,377 13,31 11,49 8,23

929,5 16,51 1,62 1,394 13,49 15,54 8,05

935,8 16,62 1,84 1,376 13,40 12,05 8,15

13 Rotary 950,8 17,42 1,99 1,430 14,00 13,80 7,71



TPM mg/tp

Water mg/tp

Nicotine mg/tp

NFDPM mg/tp

CO mg/tp

Puffs/tp

951,4 17,63 1,92 1,415 14,30 13,80 7,78

951,1 17,13 2,16 1,404 13,57 13,70 7,63

949,3 17,22 1,79 1,449 13,98 14,00 7,62

949,0 17,53 2,07 1,457 14,00 14,10 7,68

14 Linear

939,0 16,90 1,58 1,498 13,83 12,70 7,78

939,0 16,67 1,29 1,515 13,87 11,78 7,90

943,0 17,18 1,13 1,510 14,54 12,70 7,85

941,0 17,43 1,23 1,570 14,63 12,48 8,13

939,0 18,08 1,83 1,578 14,67 12,48 7,85

15A Linear

937,8 16,46 1,62 1,409 13,44 12,58 7,93

944,0 16,24 1,61 1,431 13,20 12,87 8,05

944,6 17,06 1,61 1,440 14,01 12,61 7,75

938,2 16,98 1,62 1,455 13,91 12,73 7,84

941,0 16,98 1,63 1,466 13,88 12,73 7,84

15B Rotary

951,7 16,97 1,59 1,461 13,92 12,85 7,75

951,6 16,92 1,54 1,446 13,93 12,54 7,78

949,7 17,71 1,57 1,514 14,62 12,84 7,79

950,1 17,23 1,66 1,475 14,10 12,66 7,78

944,6 16,78 1,62 1,423 13,74 12,58 7,92

16 Rotary

942,0 17,02 1,96 1,373 13,69 – 7,56

943,0 16,95 1,77 1,374 13,81 – 7,53

938,0 17,19 2,02 1,393 13,78 – 7,56

946,0 17,03 2,00 1,397 13,63 – 7,73

943,5 17,24 2,19 1,389 13,66 – 7,71

17A Rotary

948,8 16,88 1,82 1,458 13,60 13,63 8,53

946,9 16,84 1,85 1,445 13,55 13,59 8,52

945,8 16,76 1,83 1,457 13,47 13,53 8,50

946,7 16,80 1,84 1,443 13,52 13,62 8,50

949,0 16,88 1,81 1,426 13,64 13,57 8,57

17B Rotary

948,5 16,85 1,85 1,424 13,58 13,54 8,51

947,0 16,80 1,82 1,448 13,53 13,55 8,56

951,0 16,90 1,82 1,446 13,63 13,51 8,57

949,0 16,87 1,83 1,445 13,60 13,56 8,54

949,3 16,88 1,85 1,442 13,59 13,49 8,52

18A Rotary

940,2 16,83 1,77 1,456 13,60 13,39 7,46

939,7 16,95 1,77 1,456 13,73 13,47 7,47

939,1 16,86 1,81 1,455 13,59 13,38 7,42



TPM mg/tp

Water mg/tp

Nicotine mg/tp

NFDPM mg/tp

CO mg/tp

Puffs/tp

941,2 16,86 1,79 1,454 13,61 13,52 7,55

939,1 16,92 1,81 1,453 13,66 13,42 7,52

18B Rotary

938,1 17,02 1,79 1,455 13,77 13,38 7,57

939,8 17,05 1,78 1,458 13,81 13,59 7,54

937,7 17,00 1,82 1,455 13,73 13,36 7,55

940,6 17,16 1,81 1,457 13,90 13,33 7,58

940,1 17,22 1,79 1,460 13,97 13,55 7,54

19 Linear

949,2 17,65 1,45 1,403 14,80 13,58 8,27

951,3 17,13 1,37 1,325 14,43 11,95 8,27

947,5 17,88 1,56 1,398 14,92 13,08 8,03

951,6 17,25 1,54 1,495 14,22 13,10 7,83

947,1 17,18 1,45 1,508 14,22 13,23 7,85

20A Rotary

938,0 16,69 1,97 1,360 13,36 13,00 7,35

945,0 16,98 1,99 1,420 13,57 13,26 7,66

941,0 17,03 1,72 1,390 13,92 13,32 7,63

944,0 17,28 2,09 1,400 13,79 13,29 7,91

942,0 16,98 2,00 1,410 13,57 13,32 7,59

20B Rotary

938,0 17,08 2,07 1,410 13,60 13,10 7,45

944,0 17,26 1,95 1,420 13,89 13,32 7,62

941,0 17,15 2,02 1,380 13,75 13,47 7,48

942,0 17,19 2,03 1,410 13,75 13,61 7,53

942,0 17,10 1,97 1,430 13,70 13,61 7,38

21A Rotary

945,2 16,60 1,80 1,410 13,39 12,20 7,50

943,8 16,40 1,80 1,390 13,21 12,50 7,50

945,2 16,40 1,60 1,420 13,38 12,00 7,60

949,0 16,80 1,90 1,400 13,50 12,60 7,60

947,7 16,70 1,60 1,380 13,72 11,80 7,60

21B Rotary

947,4 17,00 1,90 1,460 13,64 13,00 7,70

939,1 16,60 1,90 1,420 13,28 13,30 7,60

944,1 16,10 1,40 1,380 13,32 12,90 7,50

941,0 16,40 1,40 1,390 13,61 12,90 7,50

951,1 17,10 2,00 1,430 13,67 13,70 7,80

22A Linear

954,9 16,73 1,55 1,479 13,70 12,22 7,60

934,8 16,82 1,60 1,507 13,72 12,67 7,65

933,8 16,48 1,65 1,469 13,36 12,79 7,55

932,2 16,49 1,53 1,475 13,48 13,07 7,35

934,3 16,40 1,80 1,509 13,08 12,27 7,68



TPM mg/tp

Water mg/tp

Nicotine mg/tp

NFDPM mg/tp

CO mg/tp

Puffs/tp

22B Rotary

926,1 15,79 1,97 1,473 12,34 11,88 7,03

928,2 15,17 1,55 1,445 12,17 12,59 7,26

933,2 16,06 2,12 1,494 12,44 12,48 7,25

934,0 16,09 1,77 1,481 12,84 11,97 7,16

933,5 16,37 2,10 1,494 12,78 12,70 7,35

23A Linear

945,0 17,57 1,26 1,433 14,76 14,06 7,90

942,0 17,07 1,29 1,407 14,37 13,10 7,83

944,0 17,07 1,31 1,413 14,36 13,29 8,00

940,0 17,29 1,28 1,419 14,59 13,65 7,75

948,0 17,25 1,37 1,434 14,46 13,36 7,95

23B Rotary

945,0 17,23 1,93 1,423 13,88 13,61 7,53

944,0 16,59 1,75 1,352 13,49 13,56 7,35

940,0 16,81 1,74 1,386 13,68 13,49 7,45

941,0 17,29 2,07 1,420 13,81 13,59 7,42

951,0 16,98 2,06 1,400 13,53 13,46 7,45

24 Rotary

947,0 17,20 2,22 1,452 13,52 13,72 7,59

945,0 17,48 2,42 1,459 13,60 13,98 7,55

946,0 17,50 2,29 1,469 13,74 13,69 7,63

941,0 16,75 2,15 1,406 13,20 13,60 7,38

947,0 16,67 2,09 1,424 13,16 13,39 7,36

25 Linear

937,5 17,92 1,36 1,557 15,00 13,37 8,15

928,3 17,60 1,42 1,536 14,64 13,43 8,18

930,9 17,95 1,74 1,549 14,66 13,86 8,14

933,6 16,85 1,74 1,549 13,56 9,95 7,88

926,8 17,99 1,96 1,542 14,49 11,21 7,95

ISO 20778

2 Linear

– 43,40 11,10 3,192 29,11 24,04 11,05

– 44,81 11,79 3,236 29,79 24,05 10,86

– 44,36 11,29 3,261 29,81 23,82 10,95

– 45,33 11,94 3,289 30,10 24,49 11,00

– 43,36 11,23 3,208 28,92 23,23 10,99

3 Linear

– 50,12 13,46 3,190 33,47 23,69 10,59

– 50,49 13,54 3,287 33,66 23,33 10,85

– 49,91 12,16 3,180 34,57 23,13 10,63

– 51,36 12,28 3,231 35,85 23,68 10,76

– 53,21 12,92 3,226 37,07 23,60 10,58

4 Rotary – 43,33 10,75 3,308 29,27 25,47 10,76



TPM mg/tp

Water mg/tp

Nicotine mg/tp

NFDPM mg/tp

CO mg/tp

Puffs/tp

– 42,02 9,89 3,163 28,97 25,09 10,52

– 41,65 9,60 3,255 28,97 24,57 10,99

– 41,53 9,81 3,242 28,48 24,71 10,70

– 40,96 8,56 3,264 29,14 25,51 10,91

7 Linear

– 40,45 9,07 2,971 28,41 22,93 10,42

– 45,11 11,14 3,005 30,97 23,89 10,92

– 46,40 11,37 3,239 31,79 24,57 10,70

– 42,52 9,76 3,131 29,64 23,32 10,81

– 43,78 9,73 3,095 30,95 23,54 10,78

8A Linear

– 45,00 12,09 3,163 29,76 24,61 10,61

– 44,45 11,80 3,116 29,53 24,30 10,77

– 45,86 12,56 3,191 30,11 24,78 10,81

– 45,48 12,17 3,157 30,15 24,10 10,55

– 44,96 12,10 3,139 29,72 23,94 10,77

8B Rotary

– 38,59 8,91 2,895 26,78 23,92 10,52

– 37,84 8,61 2,879 26,35 25,38 10,54

– 37,93 8,72 2,830 26,39 24,56 10,37

– 38,71 8,97 2,906 26,84 24,56 10,37

– 38,78 8,80 2,921 27,06 24,67 10,47

9 Rotary

– 40,94 8,70 3,123 29,12 25,01 10,73

– 40,30 8,91 3,119 28,27 24,95 10,69

– 40,64 9,28 3,008 28,36 24,26 10,59

– 41,82 9,89 3,104 28,83 25,07 10,82

– 41,72 9,86 3,106 28,75 24,42 10,81

13 Rotary

– 41,31 9,80 3,028 28,48 25,16 11,03

– 41,68 9,75 3,027 28,91 25,30 10,91

– 42,44 10,01 3,018 29,42 25,25 10,93

– 41,68 10,01 3,008 28,66 24,90 10,82

– 41,07 9,77 2,955 28,34 24,75 10,86

14 Linear

– 46,39 13,08 3,231 30,08 23,74 11,09

– 45,34 12,12 3,164 30,05 23,46 10,71

– 46,13 12,71 3,234 30,19 24,09 10,94

– 46,04 12,30 3,189 30,55 23,88 11,03

– 46,15 12,54 3,186 30,43 23,99 11,03

15A Linear

– 46,79 10,72 3,184 32,89 25,06 10,84

– 46,06 10,30 3,178 32,58 25,32 10,96

– 45,39 10,73 3,176 31,48 24,90 10,86



TPM mg/tp

Water mg/tp

Nicotine mg/tp

NFDPM mg/tp

CO mg/tp

Puffs/tp

– 45,46 10,61 3,170 31,68 25,47 10,95

– 46,99 10,39 3,230 33,38 24,44 11,23

19 Linear

– 45,94 10,79 2,684 32,46 24,36 11,73

– 44,99 10,62 2,768 31,61 24,91 11,26

– 45,50 10,48 2,970 32,06 23,60 11,34

– 44,63 10,34 2,785 31,50 24,41 11,35

– 43,70 9,80 2,959 30,94 24,23 11,10

22A Linear

– 45,85 11,95 3,199 30,70 24,24 10,36

– 45,06 11,83 3,189 30,04 24,15 10,81

– 46,02 11,83 3,224 30,97 24,87 10,99

– 45,68 11,56 3,202 30,92 25,24 10,90

– 44,56 10,86 3,170 30,53 24,47 11,05

23A Linear

– 45,97 11,35 3,024 31,60 25,71 11,10

– 45,80 11,75 3,040 31,00 24,92 10,94

– 44,71 11,37 2,998 30,34 24,39 11,16

– 45,71 11,89 3,045 30,78 25,46 11,04

– 44,68 10,65 3,036 31,00 24,95 11,03

23B Rotary

– 40,64 8,31 2,959 29,37 24,88 10,83

– 41,13 9,14 2,982 29,02 25,30 10,80

– 40,97 8,92 2,948 29,11 25,21 10,64

– 41,43 9,09 2,994 29,34 25,49 10,58

– 41,38 9,24 3,031 29,12 25,35 10,81

25 Linear

– 47,81 13,17 3,204 31,43 24,90 11,27

– 47,46 12,89 3,252 31,32 23,98 11,41

– 49,29 12,89 3,348 33,05 24,89 11,50

– 45,25 11,50 3,082 30,67 22,40 11,25

– 46,47 12,33 3,154 30,98 22,94 11,13


APPENDIX C – Graphs of Results







Count (p

uffs/tp)


APPENDIX D – Graphs of Z-Scores




Puff C

ount

Documents

Smoke Analysis Sub-Group (Routine Analytical Chemistry Sub