S.N.R. SONS COLLEGE (Autonomous) · 2020. 1. 3. · B. Sc. Biotechnology (2015 Batch) Semester III III 15BT301-Core IV – Basics of Immunology 5 25 75 100 5 III 15BT302-Allied :

B. Sc. Biotechnology (2015 Batch)

S.N.R. SONS COLLEGE (Autonomous) (Affiliated to Bharathiar University)

(Re-Accredited with „A‟ Grade by NAAC)

(An ISO 9001:2008 Certified Institution)

S.N.R. College Road, Coimbatore-641 006, Tamil Nadu, India.

Phone: (0422) 2562788; 256083, Fax: (0422) 2560387. Website: www.snrsonscollege.org

“Scheme of Examination along with Distribution of Marks and Credits”

B.Sc. BIOTECHNOLOGY

(For the students admitted during the academic year 2015 - 2016 onwards)

SCHEME OF EXAMINATIONS: CBCS PATTERN (WITH 2 SEM LANGUAGE PAPERS)

Part Study Components and Course Title

Instructional

Hours/

Week

CIA

Comprehen

-sive Exam Total Credit

Semester I

I 15T01/15H01/ 15F01/ 15M01- Language-I 5 25 75 100 3

II 15E01-English-I 5 25 75 100 3

III 15BT101-CORE I – Cell and Molecular

Biology 4 25 75 100 5

III 15BT102-CORE II – Microbiology 4 25 75 100 5

III 15BT103-ALLIED PAPER I– Chemistry I 4 25 75 100 4

III 15BT104-Practical I – Lab in Cell Biology

and Microbiology 3

40 60 100 3

III 15BT105-Allied Practical I – Chemistry

Practical I 3

40 60 100 3

IV 15ES01-Environmental Studies# 2 50* -

50* 2

700 28

Semester II

I 15T02/15H02/ 15F02/ 15M02-Language-

II 5 25 75 100 3

II 15E02- English-II (Communication Skills) 5 25 75 100 3

III 15BT201- CORE III – Biomolecules 4 25 75 100

5

III 15BT202 - ALLIED PAPER II–

Chemistry II 4

25 75 100 4

III 15BT203-Practical II – Lab in

Biomolecules 3 40 60

100 3

III 15BT204-Allied Practical II –

Chemistry Practical II 3 40 60

100 3

III Supportive Course I 4 25 75 100 4

IV 15VE01-Value Education –

Human Rights # 2 - 50* 50* 2

700 27


Semester III

III 15BT301-Core IV – Basics of

Immunology 5 25 75 100 5

III 15BT302-Allied : III - Office Automation 5 25 75 100 4

III Elective : I 5 25 75 100 4

III 15BT304-Skill based Subject : I Industrial

Biotechnology 5 25 75

100 3

III 15BT305-Practical III –

Lab in Immunology 4 40 60

100 3

III 15BT306-Allied Practical III –

Lab in Office Automation 4 40 60

100 3

IV

15BCT01-* Basic Tamil I @ /

15ADT01- Advanced Tamil I # (OR)

15NM01- Non-major elective - I – Personality Development #

2

75 -

75* 2 - 75*

- 75*

600 24

Semester IV

III 15BT401-Core V – Recombinant DNA

Technology 5 25 75 100 5

III 15BT402-Allied : IV – Bioinformatics and

Biostatistics 5 25 75

100 4

III 15BT403-Skill based Subject : II -

Bioinstrumentation 5 25 75

100 3

III 15BT404-Practical IV – Lab in

Recombinant DNA Technology 4 40 60

100 3

III 15BT405-Practical V – Lab in

Bioinformatics 4 40 60

100 3

III Supportive Course II 5 25 75 100

4

IV

15BCT02-* Basic Tamil II @ /

15ADT02- Advanced Tamil II # (OR)

15NM02- Non-major elective -II -

General Awareness #

2

75 -

75* 2 - 75*

- 75*

600 24

Semester V

III 15BT501-Core VI –

Environmental Biotechnology 6 25 75 100 5

III Elective –II 6 25 75 100 4

III 15BT503-Skill based Subject : III -

Enzymology 6 25 75

100 3

III 15BT504-Practical VI –

Lab in Environmental Biotechnology 6 40 60

100 3

III 15BT505-Practical VII –

Lab in Industrial Biotechnology 6 40 60

100 3

500 18


Semester VI

III 15BT601-Core VII – Plant Biotechnology

and Animal Biotechnology 6 25 75 100 5

III 15BT602-Skill based Subject : IV –

Genomics and Proteomics 6 25 75

100 3

III 15BT603-Practical VIII – Lab in Plant

Biotechnology and Animal Biotechnology 6 40 60

100 3

III 15BT604-Practical IX –

Lab in Clinical Biochemistry 6 40 60

100 3

III Supportive Course III 6 25

75

100 4

V 15EA01-Extension Activities @ - 50 - 50* 1

500 19

@ No Comprehensive Examinations. Only Continuous Internal Assessment (CIA)

# No Continuous Internal Assessment (CIA). Only Comprehensive End Semester Examinations.

List of Elective papers (Can choose any one of the paper as electives)

Elective – I 15BT303 Microbial, Human Genetics

15BT303A Genetics

15BT303B Genomics

Elective – II 15BT502 Basics of Bioprocess Technology

15BT502A Biological Chemistry

15BT502B Biophysics

Supportive Course

S.No SC- I SC – II SC - III

1 First aid and Safety Food Microbiology Clinical Biotechnology

2 Life style and Health Industrial Microbiology Medical Biochemistry

3 Human Physiology Genetic Engineering Herbal Technology


Summary

Part Subject Papers Credit Total credits Marks Total marks

Part I Languages 2 3 6 100 200

Part II English 2 3 6 100 200

Part III

Core 9 3 27 100 900

7 5 35 100 700

Supportive 3 4 12 100 300

Allied 4 4 16 100 400

3 3 9 100 300

Electives 2 4 8 100 200

Part III Skill Based 4 3 12 100 400

Part IV Lang. & Others 4 2 8 50 250*

Part V Extension 1 1 1 50 50*

Total 140 3600

* Not included in CGPA

Chairperson

Board of Studies- Department of Biotechnology

SNR SONS COLLEGE (AUTONOMOUS)

COIMBATORE – 641 006

Unit – I

Salient features of prokaryotic and eukaryotic cells, structure of microbial, plant and

animal cell, Cell theories, Structure and functions of plasma membrane, osmosis and

diffusion across membranes, active & passive transport, exo and endocytosis, microvilli,

desmosomes, gap & tight junctions.

Unit – II

Structure and functions of cell organelles – Endoplasmic reticulum, Mitochondria,

golgi apparatus, Ribosomes, Chloroplast, Centrioles, Lysosomes.

Unit – III

Structure and functions of nucleus, nuclear membrane, nucleolus, DNA, RNA types of

RNA, Structure and functions of chromosomes - giant chromosome - polytene & lamp

brush chromosome.

Unit –IV

DNA as the genetic material - transfomation, conjugation, transduction, recombination,

cell division – amitosis, mitosis, meiosis, comparison between mitosis and meiosis.

Unit V

DNA replication, transcription, translation, gene regulation in prokaryote & eukaryote,

mutations and different types, chromosomal aberrations & disorders, carcinogenesis -

cancer cells, Oncogenes.

PROGRAMME B.Sc. BIOTECHNOLOGY

SEMESTER ONE

SUBJECT CELL AND MOLECULAR BIOLOGY

COURSE CODE 15 BT 101

TEXT BOOK:

1. Cells and Molecular Biology – De Robertis, , Blaze Publishers & Distributors Pvt

Ltd, New Delhi,1990, 2nd edition.

REFERENCES

1. Molecular Biology - Phillip Sheeler, Donald E. Bianchi, John Wiley and Sons

Inc, 3rd edition, 1987.

2. Molecular Biology - Lodish, Freeman and Company, 1989, 5th edition.

3. Molecular Biology of Cell – Bruce Alberts, Garland Science, 4th edition, 2002.

4. Cell and Molecular Biology – Gerald Karp, John Wiley and Sons, 2002, 3rd edition.

UNIT – I

History of Microbiology – Discovery of Microbial World, Controversy over Spontaneous

Generation, Microbes and Human diseases. Diversity of the Microbial world – Methods

of classifying and identifying microorganisms.

UNIT – II

Development of Techniques for studying microbial pathogens – Koch’s Culture media,

Types of media, Isolation of Pure Cultures, Sterilization and Disinfection, Types of

Staining – Simple, Differential, Gram, Acid Fast and Structural Staining. Microbial

Growth – Growth curve, Measurement of Growth, Influence of Environmental growth

factors.

UNIT – III

Bacteria: Classification, Size, Shape, Arrangement and Structures external to cell wall –

Flagella, Pili, Capsules, Sheath. Cell wall structure, Composition and Characteristics.

Reproduction and Growth curve of bacteria. Algae – Classification Morphology,

Reproduction.

UNIT - IV

Fungi – Morphology, Characteristics and Importance of Fungi. Reproduction – Sexual

and Asexual. Classification of Fungi. Lichens and Micorrhiza. Industrial uses of Yeast.

UNIT – V

Viruses – Structure and Characteristics, Plant viruses and Animal viruses. Viruses and

Cancer. Prions and Viroids, Lytic and Lysogenic cycles. Microbial Interactions;

Symbiosis, Mutualism, Commensalism and Parasitism.

PROGRAMME B.Sc., BIOTECHNOLOGY

SEMESTER ONE

SUBJECT MICROBIOLOGY


TEXT BOOK:

1. Microbiology – Prescott, Harley and Klein, 2007, 6th edition, WmC Brown Publishers.

2. Microbiology – Pelczar, 2008, 37th edition, Tata Mc Graw Hill.

REFERENCES:-

1. Microbiology – An Introduction – Gerald J Tortora, Funke R, L Case, Dorling

Kindersley India Pvt Ltd, 8th edition, 2004.

2. General Microbiology – Stanier, Ingraham Wheelis, Painter, Mac Millan Press Ltd, 5th

edition, 2004.

3. General Microbiology – Volume II – Powar and Daginwala, Himalayas Publishing

House, 1992, 2nd edition.


SEMESTER ONE

SUBJECT ALLIED –I

CHEMISTRY- I


UNIT I:

Chemical bonding: Molecular orbital theory, bonding and anti-bonding Orbital, MO

configuration of Hydrogen, Nitrogen, Oxygen, Fluorine. Bond Order, Diamagnetism and

Paramagnetism. Diborane: Preparation, properties and structure. Preparation and uses of

NaHB4

UNIT II:

Interhalogen Compounds: ICl, BrF3, IF5 – Preparation, properties, Hybridization,

Structure and shape. Basic property of Iodine. Sodium Hydrosulphite, Peracids of

Sulphur: Preparation, properties, Structure and Uses.

UNIT III:

Synthesis, properties and Uses of Silicones. Fuel Gases, Natural gas, Water gas, Semi

water gas, Carburetted water, producer gas, oil gas. Fertilizers: Urea, Ammonium

Sulphate, Ammonium nitrate, Potassium Nitrate, NPK fertilizer, Triple super phosphate.

UNIT IV:

Dyes – Terms used, One example each for azo and Triphenyl methane dyes –

Preparation. Chemotherapy: Preparation, uses and mode of action of Sulpha drugs.

Structure and Uses of Penicillin and Chloromycetin. Sources and Deficiency of Vitamins

A, B, C, D, E and K. Peptide Hormones – Oxytocin, Insulin and their biological function.

UNIT V:

Pollution of air, water and soil sources, effects and remedies. Thermal radiation and

Noise – Sources, Effects and Control.

TEXT BOOK:

1. Chemistry – Dr. Veeraiyan, Paper I, High Mount Publishing House, Chennai, 2nd

edition, 2005.

REFERENCE:

1. Principles of Physical Chemistry – Puri Sharma and Pathania, , Vishal Publishing

Company, Millennium edition, 2000.

2. Text book of Inorganic Chemistry – P.L. Soni and Mohan Katyal, , Sultan Chand and

Sons, Revised Edition, 1991.


SEMESTER ONE

SUBJECT PRACTICAL –I

LAB IN CELL BIOLOGY AND MICROBIOLOGY


1. Microscopy and its parts

2. Identification of cell types (Permanent slides)

3. Identification of different phases of cell division (permanent slides) – Onion Root tip

(Skill Oriented)

4. Osmosis through semi permeable membrane

5. Sterilization techniques

6. Media preparation – Liquid and Solid media

7. Culture techniques – Pour plate method

Streak plate method

Spread plate method

8. Simple staining (Skill Oriented)

9. Gram staining (Skill Oriented)

10. Isolation of Bacteria, Fungi from soil

11. Negative Staining

12. Antibiotic Susceptibility Test by Disc Diffusion Method

References:

1. Experimental Procedures in Life Sciences – Dr. S. Rajan and Mrs. R. Selvi Christy,

Anjana Book House, 1st edition, 2010.


SEMESTER ONE

SUBJECT ALLIED PRACTICAL –I

CHEMISTRY PRACTICAL –I


Volumetric Analysis:

1. Estimation of Sodium hydroxide using Standard Carbonate.

2. Estimation of Hydrochloric acid using Standard Oxalic acid.

3. Estimation of Oxalic acid using Standard Sulphuric acid.

4. Estimation of Ferrous Sulphate using Standard Mohr’s salt solution.

5. Estimation of Oxalic acid using Standard Ferrous Sulphate.

6. Estimation of Potassium permanganate using Standard Sodium Hydroxide.

PART – IV

SEMESTER – I

ENVIRONMENTAL STUDIES

Course Code: 15 ES 01 Comprehensive Examination: 50 Marks

Instruction Hour per Week: 2

UNIT – I The multidisciplinary nature of environmental studies

UNIT – II Ecosystems

UNIT – III Biodiversity and its conservation

UNIT – IV Environmental pollution

UNIT – V Social issues and the environment

Text Book:

Environmental Studies for under graduate courses – Bharathiar University

Reference Book:

Environmental Education – V.K. Nantha, 2002, 2nd edition, DPH, New Delhi – 02.

A Text book of Environmental Studies – Thangamani. A and T. Shymama, 1996, 2nd

edition, DPH, New Delhi – 02.


SEMESTER TWO

SUBJECT BIOMOLECULES


UNIT-I

Biomolecules – chemical composition and properties. Water and its interaction with

Biomolecules, hydrogen bonding and solubility - physical properties. Ionization of water – pH and

pKa, titration curve - Henderson equation.

UNIT-II

Carbohydrates - Monosaccharide, Disaccharides and Polysaccharides - Classification, Structure

and Properties.

UNIT III:

Lipids – Classification, Structure, Properties and Reactions of fatty acids, Phospholipids,

Glycolipids, Sterols.

UNIT IV:

Amino acids - Classification, Structure, Peptides - Structure of Peptide bonds, Proteins -

Classification and Organization - Primary, Secondary, Tertiary.

UNIT V:

Nucleic acids – Constituents and Forms of DNA and types of RNA, Primary, Secondary and

tertiary structure of DNA, Functions and properties of Nucleic acids.

TEXT BOOK:

1. Fundamentals of Biochemistry – Dr. U. Sathyanarayana, Dr. U. Chakrapani, Books

and Allied (P) Ltd, 1st edition, 2008.

2. Principles of Biochemistry – Lehninger, Nelson, Cox CBS Publication, W.H.

Freeman and Company, 4th edition, 2005.

REFERENCES:

1. Fundamentals of Biochemistry – Voet and Voet John Wiley and Sons, Inc, 1992,

2nd edition.

2. Principles of Biochemistry – Smith, McGraw –Hill International Cook company,

8th edition, 2005.

3. Biochemistry – Stryer.L, W.H. Freeman & Co, 5th edition, 2002.


SEMESTER TWO

SUBJECT ALLIED –II -CHEMISTRY II


UNIT I:

General Methods of Extraction of Metals. Types of Ores, Methods of Ore dressing, Types of

furnaces, Reduction methods, Electrical methods, Types of refining, Van Arkel Zone refining.

Role of carbon in Steel. Heat treatment of Steel.

UNIT II:

Aromatic Compounds: Electrophilic Substitution in Benzene, Mechanism of Nitration,

Halogenation, Alkylation, Acylation, Sulphonation. Isolation, Preparation, Properties and

Structure of Naphthalene.

.

UNIT III:

Heterocyclics: Preparation and properties of Furan, Thiophene, Pyrrole and Pyridine. Preparation

and Properties of Glucose and Fructose. Interconversion of Glucose and Fructose.

UNIT IV:

Energetics: First Law of Thermodynamics, Types of Systems. Reversible, irreversible, isothermal

and adiabatic processes. Spontaneous Processes, Joule- Thomson effect, Enthalpy, Bond energy.

Need for Second law.

UNIT V:

pH determination, Buffer action in living system, Electro Plating and its applications, Hydrolysis

of salt, Conductometric titrations.

TEXT BOOK:

1. Chemistry – Dr. Veeraiyan, Paper I, High Mount Publishing House, Chennai, 2nd

edition, 2005.

REFERENCE:

1. Principles of Physical Chemistry – Puri Sharma and Pathania, Vishal Publishing

company, Millennium edition,2000.

2. Organic Chemistry- Morrison and Boyd, Pearson Education, 6th edition, 1992.

1.Qualitative analysis of carbohydrates - Monosaccharides (Glucose, Galactose,

Fructose)

Disaccharides (Sucrose)

Polysaccharides (Starch)

2. Qualitative analysis of amino acids – Cystiene, Methionine, Arginine, Aspartic acid.

3. Characterization of protein by Biuret method.

4. Estimation of protein by Lowry’s method.

5. Estimation of total free amino acids by Ninhydrin Method.

6. Qualitative analysis of lipids - soap formation.

7. Estimation of acid number of oil.

8. Paper chromatography.

9. Estimation of Glucose by O - Toluidine Method

10. Determination of total Carbohydrate by Anthrone Method

REFERENCES:

1. Biochemical Methods- S. Sadasivam and A. Manickam, 2nd edition


SEMESTER TWO

SUBJECT PRACTICAL – II

LAB IN BIOMOLECULES


1. Detection of Elements. (N, S, halogens)

2. To distinguish between Aliphatic and Aromatic, Saturated and Unsaturated.

3. Functional Group tests for Phenol, acids (mono and di), Aromatic Primary amine,

amide, diamide, dextrose, systematic analysis of Organic compounds containing One

Functional group and Characterization by Confirmatory test.


SEMESTER TWO

SUBJECT ALLIED PRACTICAL – II

CHEMISTRY PRACTICAL – II


Unit – I

History of First aid, First aid kit, The Red Cross. Blisters and bruises, Wounds – Types of wounds,

Caring for minor and major open wounds, Special problems. Fracture – open fracture, dislocation,

sprains, strains, How to make a sling. Severe bleeding.

Unit – II

Poisoning: What is poison, What to do when injested, inhaled, adsorbed. Animal bites – minor

wounds, deep wounds, infection, suspected rabies. Insect stings, spiders and snake bites.

Unit – III

Burns: Minor burns - First degree, Second degree, Third degree burns, Major burns Electric burns,

Chemical burns. Cold burns and frost bites. Heat exhaustion and heat stroke – Signs and

Symptoms, Precautionary and safety measures.

Unit – IV

Cardiopulmonary resuscitation (CPR) – Assessing the situation, breathing for the person restore

blood circulation, CPR on a child and on an infant – airway, breathing, circulation. Choking – in

case of conscious and unconscious adult infants and children in conscious and unconscious.

Unit – V

General emotional first aid – reaching out physically and emotionally, protection, reassurance,

reinforcement, fainting, shock – causes, symptoms, and help. Anaphylaxis – Foreign body in eyes,

ears, skin, inhaled, ingested.


SEMESTER TWO

SUBJECT (SC) FIRST AID AND SAFETY

COURSE CODE


SEMESTER TWO

SUBJECT (SC) LIFE STYLE AND HEALTH

COURSE CODE

Unit – I

NEURAL CELL – Mental process in the brain – Elementary operation – Regions of brain –

Differential function – Nervous system – Two classes of cells – Nerve cells as the signal unit of

behaviour, Electrical properties of Nervopus, Neutral basis of perception.

Unit – II

CONCIOUSNOUS: Photo transduction and information processing in the routine sleep and

dreaming – Neural mechanism for the sleep – Wake cycle – Sleep and Consciousness – Insomnia;

Sleep apnea, Narcolepsy: Loss of consciousness.

Unit – III

INDIVIDUALITY: Genetic determinants of behaviour – Role of genes bioethical complication;

Language vehicle; Speech development, learning and memory – Neural basis of memory;

Biological basis of individuality and behaviour; Environmental inference on behaviour.

Unit – IV

Disorders, pain and Analgesia, Migraine, Addiction, Behavioural stress, Autism, Myasthenia

gravis and Nervous system. Alzhemier’s disease and nervous system, Parkinson diseases and

nervous system, Current therapy available. Food habits – Nutritional deficiencies – Disorders –

Smoking and Alcohol – Liver and respiratory disorders, Cosmetics – Skin allergies.

Unit – V

Mind – Mapping the mind – State of mind, Scanning the brain, search for the mind, chemical

messengers, co-existence of transmitters; Cognitive fluidity, splitting the brain, anatomy of joy

and pain, Sensation – The sixth sense, illusions pattern in the mind, eye

talk, music and mind, What is meant by states of mind, making memories, consciousness,

Computer – Brain – understanding.

REFERENCES:

1. Mapping the Mind – Carter, Weidenfeld and Nicolson, 1995, Revised Edition.

2. Principles of Neural Science – Kandel, E.R., 2001, 3rd edition.


SEMESTER TWO

SUBJECT (SC) HUMAN PHYSIOLOGY

COURSE CODE

Unit – I

Digestive System – Structure and functions – microvilli, Digestive enzymes and digestive juices,

Digestion of carbohydrates, proteins and lipids. Respiratory system – Lungs – Structure and

functions, Gas exchange.

Unit – II

Circulatory System – Heart – Structure and functions. Blood – RBC, WBC, Blood coagulation,

Blood group and Blood transfer.

Unit – III

Nervous System – Structure of Neuron, Action potential, Synaptic transmission. Structure of eye

– Receptor mechanism of eye.

Unit – IV

Excretory System – Structure of Kidney, Nephron – Function and mechanism, Mechanism of

Urine formation (Micturition) Mechanism of Excretion.Reproductive System – Spermatogenesis,

Structure of Ovaries, Ovarian and Menstrual cycle.

Unit – V

Muscle cells – Striated and Non-striated muscle fibre – Mechanism and muscle contraction.

Theories of muscle. Endocrinology: Pituitary gland, Adrenal gland, Pineal gland. Hormones:

Progesterone, Oestrogen, Aldosterone.

REFERENCES

1. Human Anatomy and Physiology – Kent M.Van De Graff et al, , Tata McGraw Hill,

2004, 2nd edition.

2. Ross and Wilson Anatomy and Physiology in Health and Illness – Anne Waugh and

Allison Grant, 12 edition, International edition, 2014.

PART – IV

SEMESTER – II

VALUE EDUCATION – HUMAN RIGHTS

(2 Hours per week)

UNIT I: Concept of Human Values, Value Education Towards Personal Development

Aim of education and Value Education; Evolution of value oriented education; Concept

of Human values; types of values; Components of Value education

Personal Development:

Self analysis and introspection; Sensitization towards gender equality, Physically

challenged, intellectually challenged. Respect to – age, experience, maturity, family members,

neighbors’, co-workers.

Character Formation towards Positive Personality:

Truthfulness, Constructivity, Sacrifice, Sincerity, Self Control, Altruism, Tolerance,

Scientific Vision.

UNIT II: Value Education towards National and Global Development

National and International Values:

Constitutional or national values – Democracy, socialism, secularism, equality, justice,

liberty, freedom and fraternity.

Social Values – Pity and probity, self control, universal brotherhood.

Professional Values – Knowledge thirst, sincerity in profession, regularity, punctuality

and faith.

Religious Values – Tolerance, Wisdom, Character.

Aesthetic Values – Love and appreciation of literature and fine arts and respect for the

same.

National Integration and international understanding

UNIT III: Impact of Global Development on Ethics and Values

Conflict of cross-cultural influences, mss media, cross-border education, materialistic

values, Professional challenges and compromise.

Modern Challenges of Adolescent Emotions and behavior; Sex and spirituality:

Comparison and Competition; Positive and negative thoughts.

Adolescent emotions, Arrogance, anger, sexual instability, selfishness, defiance.

UNIT IV: Therapeutic measures

Control of the mind through

a) Simplified physical exercise

b) Meditation – Objectives, types, effect on body, mind and soul

c) Yoga – Objectives, Types, Asanas

d) Activities:

i) Moralization of Desires

ii) Neutralization of Anger

iii) Eradication of Worries

iv) Benefits of Blessings.

UNIT V: Human Rights

1. Concept of Human Rights – Indian and International Perspectives

a) Evolution of Human Rights

b) Definitions under Indian and International Documents

2. Broad Classification of Human Rights and Relevant Constitutional Provisions.

a) Right to Life, Liberty and Dignity

b) Right to equality

c) Right to exploitation

d) Cultural and Educational Rights

e) Economic Rights

f) Political Rights

g) Social Rights

3. Human Rights of Women and Children

a) Social Practice and Constitutional Safeguards

i) Female Foeticide and Infanticide

ii) Physical assault and harassment

iii) Domestic Violence

iv) Conditions of Working Women

4. Institutions for Implementation

a) Human Rights Commission

b) Judiciary

5. Violations and Redressel

a) Violation by State

b) Violation by Individuals

c) Nuclear Weapons and Terrorism

d) Safeguards

Text Book:

Learning Material from Bharathiar University

Reference Books:

1. Value Oriented education – D. Dayakkara Reddy, DPH, New Delhi – 02.

2. Value Education – De. N. Venkataiah, APH, New Delhi – 02.

Free Yourself from Tension with Yoga – KR. Ganesh Babu, AUM Yoga Therapy Research

Institute, Coimbatore


SEMESTER THREE

SUBJECT BASICS OF IMMUNOLOGY


UNIT I

Introduction, innate and adaptive immunity, Hematopoiesis, cells of the immune system, primary

and secondary lymphoid organs and inflammatory mechanism.

UNIT II

Antigens, epitopes, haptens, Immunoglobulin – structure, classes and biological functions.

Antigen antibody interactions, Monoclonal antibodies – production and uses.

UNIT III

Histocompatibility complex, classes of MHC, Antigen processing and presentation, T-cell

receptor, T cell activation, Role of Cytokines in immune response.

UNIT IV

The complement components and their role in immune response, Types of vaccines and

vaccination, Hypersensitivity reactions-Type I, II, III, IV.

UNIT V

Tumor immunology, primary and secondary Immuno deficiency, AIDS, Auto immunity and auto

immune disorders, Transplantation immunology

TEXT BOOKS:

1. Immunology- A short course- Benjamin, Freeman and Company, 2nd edition, 1993.

2. Immunology by Ian R. Tizard, Saunder’s, 4th edition, 1995.

REFERENCES:

1. Immunology by Richard .A. Goldsby, Thomas Kindt, Barbara Osborne, Janis

Kuby, Freeman and Company, 1993.

2. Immunology by Roitt, Brestoff and Male, Revised edition, 1996.

3. Cellular and Molecular Immunology- A. Bul and K. Abbas, 1994.

4. Fundamentals of Immunology: Paul W.E. (Eds.) Raven Press, New York, 1988.


SEMESTER THREE

SUBJECT OFFICE AUTOMATION


UNIT I

Fundamentals of Computer – generation of Computers-Classification of Computers-Components

of Computers-input devices and output devices-memory of operating system-programming.

UNIT II

Introduction to windows-starting windows using mouse-moving, resizing, maximizing,

minimizing, restoring windows-opening multiple windows-introduction to MS office

UNIT III

MS word-Introduction to word editing a document – move and copy text-formatting text and

paragraph-finding replacing text and spelling checking-columns, tables and other features –using

mail merge.

UNIT IV

MS Excel-Introduction to work sheet- getting started with excel – editing cells- moving,

copying, inserting and deleting rows and columns creating charts.

UNIT V

Introduction to power points – creating and presentation power points views-slide shows-

animation.

REFERENCES:

1. Computer fundamentals and windows with internet technology- N.Krishnan, SCITECH

Publications.


SEMESTER THREE

SUBJECT MICROBIAL, HUMAN GENETICS

(Elective)


UNIT I

Mendelian Principles: Segregation, independent assortment, sex linkage, dominance, co-

dominance, multiple alleles, Rh & ABO incompabitibility, Epistasis, Lethality, Sex

determination and Sex Linkage, Pleotrophy.

UNIT II

Polytene Chromosomes, Lampbrush Chromosomes, Reproductive cycles of viruses, bacteria,

paramecium, Neurospora and yeast. Significance of Drosophila, tetrad analysis.

UNIT III

Replication of DNA, Synthesis of DNA, Enzymes involved, inborn errors of metabolism, protein

synthesis, genetic code. Transformation, transduction and conjugation.

UNIT IV

Chromosome variation in number; euploidy, aneuploidy, mosaics and chimeras, human

chromosomes and variation. Change in chromosome structure, deletion, duplication, inversion,

translocation, Mutation.

UNIT V

Maternal effect – extranuclear (cytoplasmic) inheritance, infectious heredity, sickle cell anemia,

haemophilia, phenylketonuria, Plasmids, transposable elements, population genetics.

TEXT BOOKS:

1. Genetics –Monroe W Strickberger, Prentice Hall of India Pvt Ltd, New Delhi, 3rd edition,

2004.

REFERENCES:

1. Human Molecular Genetics- Strachan, Prentice Hall of India Pvt Ltd, New Delhi, 3rd

edition, 2001.

2. Genetics by Goodenough, Blackwell Publishing, 2nd edition, 2004.

3. Genes IV by Benjamin Lewin, Pearson Education International, 4th edition, 2004.


SEMESTER THREE

SUBJECT GENETICS (ELECTIVE)

COURSE CODE 15 BT 303A

UNIT I

Genome organization in bacteria, plant and animals, DNA, RNA and Replication. Human

genome and its organization, Human genome project.

UNIT II

Mendel’s law of inheritance, non – Mendelian inheritance, sex determination in plants and

animals. Sex linkage, chromosomal theory of inheritance.

UNIT III

Chromosome structure in bacteria, plants and animals, specialized chromosomes, chromosome

aberrations, structure and mutation – spontaneous and induced.

UNIT IV

Fundamentals of microbial genetics – conjugation, transduction and transformation. Replica

plating technique, DNA repair mechanism,

UNIT V

Genetic diversity, genetic diseases, syndrome, prenatal diagnosis, counselling, population

genetics, genetic based evolution.

TEXT BOOKS:

1. Genetics – Monroe W Strickberger, Prentice Hall of India Pvt Ltd, New Delhi,

3rd edition, 2004.

2. Genomes – T. A. Brown Wiley- Liss Publications, Revised edition, 2002.

REFERENCES:

1. Principles of Genetics- Gardner, Wiley – Liss Publications, Revised Edition, 2002.

2. Text book of biotechnology by R.C.Dubey, S Chand and Company, New Delhi,

2nd edition, 1993.

3. Text book of biotechnology by P.K.Gupta, S Chand and Company, New Delhi,

Revised edition, 1991.


SEMESTER THREE

SUBJECT GENOMICS

COURSE CODE 15 BT 303B

UNIT I

Computational analysis of sequences – genes and regulatory regions – gene annotation,

similarity searches – pairwise and multiple alignments , prediction of gene function using

homology – ORF prediction , models and methods used in prediction.

UNIT II Construction of genetic maps – physical maps, FISH – to identify chromosome landmarks –

BAC libraries. Restriction mapping – DNA sequence assembly – comparative genomics.

UNIT III

DNA sequence databases - specialized genomic resources – NCBI. Data retrieval tools –

ENTRETZ, BLAST, OMIM.

UNIT IV

Outline of typical proteomics experiment – identification and analysis of proteins by 2D

Electrophoresis – spot visualization. Commercial databases. Sequence databases.

UNIT V

Human disease genes – allele specific probes, and hybridization based assays – ligation based

assays – proteomics and drug discovery.

TEXT BOOKS:

1) Text book of Biotechnology – R.C. Dubey, S Chand and company, New Delhi,

2nd edition, 1993.

2) Introduction to Bioinformatics – T.K. Athwoods, D.J.Parry - Smith, Pearson Education

Ltd, Low price edition, 1999.

3) Genomes – T. A. Brown, Blackwell Publishing, 3rd edition, 2006.

REFERENCES:

1) Principles of gene manipulation and Genomics – S.B.Primrose and R.M. Twyman,

Blackwell Publishing, 7th edition, 2006.

2) Molecular Biotechnology - Glick. B. R. and Pasternak .J.J., New Age International Pvt

Ltd, 3rd edition, 2005.


SEMESTER THREE

SUBJECT INDUSTRIAL BIOTECHNOLOGY


UNIT I

Isolation of Microbes, Primary and Secondary Screening of Strain and Preservation –

Subculture, Lyophilization and Inoculum Preparation.

UNIT II

Types of Fermentation – Continuous Fermentation, Submerged Fermentation, Fermentors-

Batch, Tubular, Continuous Stirred Tank, Air Lift Fermentors.

UNIT III

Antibiotics – Penicillin, Streptomycin and Tetracycline and its Production. Organic acids –

Citric acid, Acetic acid. Aminoacids production – Lysine.

UNIT IV

Vitamins – Vitamin B Complex and C. Enzyme production – Amylase, Proteinase, Pectinases,

Cellulase. Enzyme Immobilization – Characterization and Application.

UNIT V

Yeast – Brewer’s Yeast, Baker’s Yeast, Microbial Proteins – Bacterial, Fungal and Algal protein.

Recovery and Purification of Fermented products – Precipitation, Filtration, Centrifugation and

Membrane Process.

TEXT BOOKS:

1. Industrial Biotechnology – A. H. Patel, Mac Millan India Ltd, 1984.

2. Industrial Microbiology – L.E.Casida JR., New Age International Pvt Ltd, 1st edition,

1968.

REFERENCES:

1) Principles of Fermentation Technology – Stanbury and Whittaker Pergamon Press,

Oxford, 5th edition, 2006.

2) Industrial Microbiology – Wulf Crueger and Anneliese Crueger, 2004, Panima

Publishing Corporation, Revised edition, 2004.

3) Industrial Microbiology – Prescott and Dunn, Agrobios India, 2nd edition, 2005.


SEMESTER THREE

SUBJECT LAB IN IMMUNOLOGY


1. Separation of serum from blood

2. ABO blood grouping

3. Checking antisera property of blood grouping with known antigen

4. Single Radial Immuno diffusion

5. Double Immuno diffusion

6. CRP test and RA test

7. ASO test

8. Pregnancy test

9. Immuno electrophoresis

10. Rocket Immunoelectrophoresis

References:




SEMESTER THREE

SUBJECT LAB IN OFFICE AUTOMATION


MS – POWER POINT

1. Prepare an organization sheet for a college environment in Power Point.

2. Perform frame movement by inserting clip arts to illustrate running of a car automatically.

3. Prepare an Advertisement presentation for a product.

MS – EXCEL

1. Built a work sheet to perform correlation and regression coefficients using formulae and check

the answer with built in

2. Worksheet Preparation for electricity bill.

3. Draw Graphs to illustrate Class Performance

4. Worksheet for pay roll preparation for a company

MS – WORD

1. Illustrate the mail merge concept to apply for a suitable job for at least 5 companies

2. Using MS – WORD perform the following.

a) Change the font size to 20

b) Change the font type Garamond

c) Align the text to left, right, justify and center

d) Underline the text

e) Table manipulation

MS- ACCESS

1. Perform sorting on name, place and pin code of students database and list them in the sorted

order.

2. Using queries retrieve information from sales database which contains Trans – no, date, prod-

id, prod-name, qty, unit-price and region. List out records region-wise, date-wise and product-

wise.

3. Create mailing labels for employee database.

REFERENCES:

1. Computer fundamentals and windows with internet technology- N.Krishnan, SCITECH

Publications.


SEMESTER THREE

SUBJECT PERSONALITY DEVELOPMENT (NON- MAJOR)

COURSE CODE 15 NM 01

UNIT I

Personality – Definition – social and self concepts of personality – can personality be developed?

– Traits and patterns of Personality – Social Classifications of Personality, Factors affecting

Personality – Environmental factors- physical factors- intellectual factors= Emotional factors –

values – group skills. Dynamics of Personality formation – Prenatal stage – Nursery stage –

Adolescent stage – Adult Stage – Old age

UNIT II

Successful Personality – Meaning – Acquisition and qualities of a successful Personality –

Barriers to successful personality – Removing barriers to personality development. Scope,

Techniques and Principles opf Personality Development – Personality development strategy

UNIT III

Self Development - Approach – Aims and Objectives – Self appraisal – Analysis of Social

environment – Selection, Formulation and Execution of a plan for self development

Communication – Importance – Objectives- Methods – Principles and Application of

communication for Personality Development

UNIT IV

Intricacies of dressing up – Purpose and factors to consider while dressing up – forms and codes

of dress – Approach to dressing up for personality projection

Social Etiquettes – Participation in social events – Interpersonal etiquettes – Meal service and

eating manners – Social graces and customs.

UNIT V

Interviews- Classification – Self Projection in a selection board of interview, group discussion,

Group task, telecommunication, video Conferences and Public addresses.

TEXT BOOKS:

1. The Dynamics of Personality development and Projection by J.R.Bhatti, Pearson Education,

2009.

REFERENCE BOOKS:

1. Personality Development and Career Management – A Pragmatic Prospective by

R.M. Omkar, Sultan chand, 2008

2. Developing soft Skills by Robert. M. Sherfield, Rhonda. J. Montgomery and Patricia G.

Moody - Pearson Education, 2009.

3. How to Succeed in Group Discussion and personal interviews by Dr. S.K. Mandal – Jaico

Publishing House, 2005.

4. Personality Development by Dr. T. Bharathi – Neelkamal Publications Pvt Ltd, 2008.


SEMESTER FOUR

SUBJECT RECOMBINANT DNA TECHNOLOGY


UNIT I

Genetic Engineering and recombinant DNA technology- An Introduction, Basics of gene

manipulation: Tools for recombinant DNA technology, Enzymes used in gene manipulation of

DNA and RNA.

UNIT II

Isolation and purification of DNA, genomic DNA isolation from bacteria, plants and animals,

plasmid DNA isolation, phage DNA isolation.

UNIT III

Vectors: Types of vectors: cloning and expression vectors, plasmids, bacteriophages, Cosmids

and Phasmid vectors, cloning vectors (YEPs, Ylps,YRps,YCps,YAcs) and its applications.

UNIT IV

Cloning Vectors for plants- Ti and Ri plasmids, plant virus vectors, Cloning Vectors for animal

cells- SV 40, Adenovirus, Pox virus, Retrovirus vector and Baculovirus vectors. Agrobacterium

mediated gene transfer in plants.

UNIT V

Gene cloning Strategies: Cloning genomic DNA and cDNA ; screening strategies.Cloning in

bacteria other than E.coli, Saccharomyces cerevisae and other fungi.Gene targeting and Gene

silencing.

TEXT BOOKS:

1. Gene biotechnology- S.N. Jognand, Himalaya Publications, 2nd edition, 2003.

2. Gene cloning- T.A. Brown, Pergamon Press, Oxford, 2nd edition, 2005.

REFERNCES:

1. Principles of Gene manipulation – Primrose, Twymann & Old, Blackwell Scientific

Publications, 6th edition, 1994.

2. Recombinant DNA – Watson & Gilman, Scientific American books, Newyork, 2nd edition,

2005.


SEMESTER FOUR

SUBJECT BIOINFORMATICS AND BIOSTATISTICS


UNIT I

Bioinformatics – Definition, biological sequence, Importance, Information network- Internet,

World Wide Web, Web browsers, HTTP, HTML and URLS, Applications of Bioinformatics

tools in analysis.

UNIT II

Databases, Biological databases classification of databases, primary and secondary databases,

Protein information resources – Composite protein sequences databases, composite protein,

pattern database, structure classification databases.

UNIT III

Genome Information resources- DNA Sequence databases, specialized genomic resources-

NCBI, GDB, MGD, Data Retrival Tools- ENTREZ, OMIM, PubMed lower link, BLAST.

UNIT IV

Statistics: Defnition – Statistics as Data, as method, Importance, Functions and Limitations.

Collection and Presentation of data: Diagrammatic representation of data- Histogram, Frequency

polygon, Frequency curve and O give.

UNIT V

Measures of Central tendency: Mean Median and Mode. Measures of Dispersion: Mean

Deviation, Standard Deviation and Co-efficient of Variation .Simple Correlation: Scatter

Diagram –Karl Pearson’s Co-efficient of Correlation –Rank Correlation . Regression Analysis:

Simple Regression Equation –Regression Co –efficient.

TEXT BOOKS:

1. Text Book of Biotechnology –R.C.Dubey, S Chand and Company, New Delhi, 2nd edition,

1993.

2. Introduction to Bioinformatics- T.K. Attwood, D.J. Parry-Smith, Pearson education,

Low price edition, 1999.

3. Statistical methods-S.P.Gupta, Sultan Chand and Sons, 33rd edition, 2004.

4. Bioinformatics- Local authors- Vijay & K.Mani, B. Thiagarajan & PA. Vijayalakshmi,

Revised edition.

REFERENCES:

1. Bioinformatics- Sequence & Genome analysis – David Mount, Cold Spring Harbor

Laboratories Press, CSH New York, 1st edition, 2001.

2. Principles of Gene manipulation & Genomics – S.B. Primrose & R.M. Twymann, Blackwell

Publishing, 7th edition, 2006.

3. Fundamentals of foundation of Biostatistics –Khan and Khanum, Ukas Publications,

Hyderabad, Revised edition, 1994.

4. Statistics- R.S.N. Pillai, V. Bagavathi, S. Chand and Company Ltd, 17thedition, 1984.


SEMESTER FOUR

SUBJECT BIOINSTRUMENTATION


UNIT I

pH scale , methods of calculating pH from instrumentation, Henderson equation, Reference

Electrode, Glass Electrode, pH indicators, Spectroscopy and its applications.

UNIT II

Absorption and Emission Spectroscopy; ultraviolet, visible, fluorescence and phosphorescence

spectrometry, flame emission and atomic absorption spectroscopy, atomic emission

spectroscopy, Infrared spectrometry, Raman spectroscopy.

UNIT III

Principles of Chromatography, Gas Chromatography, Instrumentation, methods and applications

of High- Performance liquid Chromatography, electro analytical methods of analysis

Potentiometry, Voltammetric techniques, electro separations, coulometry and conductance

methods.

UNIT IV

Electrophoresis- Principles, materials and applications. Agarose, polyacrylamide gel and

immuno electrophoresis. Blotting methods.

UNIT V

Centrifugation- Principles, Instrumentation, units, types and applications. Radioactivity,

Fundamentals of Radioactive isotopes and Radioisotopes.

TEXT BOOKS:

1. Biochemical Engineering fundamentals, Bailey, J.E. and Ollis, D.F., Mc. Graw Hill

Publishers, New York, 2nd edition, 2005.

2. Principles and Techniques in Molecular & Cell Biology, Blackwell Publishing, Revised

edition, 2002.

3. Instrumental methods of analysis, Willard, H.H. et al, CBS Publishers, New Delhi, 1st edition,

1986.

REFERENCES:

1. Comprehensive Biotechnology: Vol.I -III by M. Moo- Young, H.W. Blanch S. Drew, and

D.I.C. Wang, Pergamon Press, 2nd edition, 1985.

2. Bioprocess Engineering Principles by P.M. Doran, Academic Press, 2nd edition, 1995.


SEMESTER FOUR

SUBJECT LAB IN RECOMBINANT DNA

TECHNOLOGY


1. Isolation of genomic DNA from cauliflower

2. Isolation of genomic DNA from goat liver

3. Isolation of genomic DNA from bacteria

4. Isolation of plasmid DNA from bacteria

5. Agarose gel electrophoresis

6. Southern blotting

7. Isolation of RNA

8. Restriction digestion of DNA

9. Preparation of Competent cells

10. Transformation

REFERENCES:




SEMESTER FOUR

SUBJECT LAB IN BIOINFORMATICS


1. Retrieval of article abstract from PubMed

2. Retrieval of a DNA sequence from GenBank

3. Retrieval and analysis of inorganic compounds using Pubchem

4. ORF prediction using ORF FINDER

5. Insilico transcription and translation of a gene

6. Identification of an unknown / protein sequence using BLAST N and BLAST P

7. 3D structure visualization using RASMOL

8. Phylogenetic tree analysis of four sequences using CLUSTALL W

9. Docking analysis

GENERAL AWARENESS

(Effective from 2015-15 and onwards- Non – major II)

COURSE CODE: 15NM02 COMPREHENSIVE EXAMINATION: 75MARKS

(To be taught during the Second year study of every undergraduate course 2 hours every week)

Course Content:

Important events/ dates in the history of the world in general and in Indian in particular

importance is also to be given to the local history (History of Tamilnadu)

World Personalities:

Great authors and their important works: Inventions and discoveries; Honours and

Awards.

Sports, Education, General Science, Geography, Important technologies, Indian

Constitution, National movements.

Skills in the following areas:

1. Logical Reasoning

2. Mental Ability

3. Numerical Ability and General Intelligence

Text Book:

Foundation Course- A: General Awareness- Bharathiar University.

Reference Book:

Objective Arithmetic - Ravi Chopra

Objective General Knowledge - Bright & Phull

Binny’s General Mental Ability Reasoning &

Logic - Kaushal Goyal

Manorama Year Book

Competition Success Review & General Knowledge - Monthlies.


SEMESTER THREE

SUBJECT (SC) FOOD MICROBIOLOGY

COURSE CODE

UNIT I

Food microbiology – scope of food microbiology, microorganisms and food materials – air borne

bacteria – fungi – microbes of soil, water, plants, and animals. Defects in fruits and vegetables,

meat, eggs, fish.

UNIT II

Microbiology of food preservation – heat processing, pasteurization, spoilage of canned food,

irradiation – microwave, UV, chilling and freezing, preservatives – nitrite, sulfur dioxide,

organic acids

UNIT III

Microbiology of milk and milk products. – microflora of raw milk , heat treatment of milk,

fermented dairy products – Yoghurt , types of cheese – swiss cheese, ripened cheese.

UNIT IV

Plant products – cereals, pulses, nuts, fruits and fruit products, vegetables and vegetable products

– olives, cucumbers. Fish – fermented fish, spoilage of fresh fish.

UNIT V

Pathogenesis of diarrhoeal diseases – Botulism, E.coli, staphylococcal food poisoning,

REFERENCES:

1) Industrial microbiology – Prescott. And Dunn’s, Gerald Reed, Agrobios India, 2nd

edition, 2005.

2) Food microbiology – M.R. Adams, M.O.Moss, New Age International Pvt Ltd, 1st

edition, 1995.

TEXT BOOKS:

1) Basic of food microbiology – George.J. Bandwat, CBS Publishers and Distributors, New

Delhi, 2nd edition, 2004.


SEMESTER THREE

SUBJECT (SC) GENETIC ENGINEERING

COURSE CODE

UNIT I

Biology and construction of plasmid vectors – p BR322, Puc18, phage as vectors- lamda phage,

cosmids, phagemids, expression vectors- shuttle vectors, artificial chromosomes: YAC, BAC.

UNIT II

Enzymes used in manipulation: polymerases and types: nucleases, endonucleases, exonucleases

and restriction enzymes.

UNIT III

Construction of a recombinant molecule. Bacterial transformation: principle and methods.

Physical methods of transformation. Analysis of cloned genes: direct and indirect methods –

Agarose Gel Electrophoresis, blotting techniques.

UNIT IV

Genomic and cDNA libraries, PCR - Principles and its types. DNA fingerprinting, RAPD, RFLP,

DNA sequencing, micro arrays.

UNIT V

Gene therapy - cystic fibrosis, DNA forensics, recombinant products, blood products, vaccines,

interferons.

TEXT BOOKS:

1) Biotechnology – Fundamentals and Applications – S.S. Purohit, Agrobios India,

4th edition, 2005.

2) Biotechnology – R.C. Dubey, S Chand and Company Ltd, New Delhi, 2nd edition, 1993.

REFERENCES:

1) Molecular biotechnology – Principles and Applications of Recombinant DNA –

Bernard R. Glick and Jack. J. Pasternak, 3rd edition, 2005.

2) Principles of Gene Manipulation – Introduction to Genetic Engineering R. W. old and

S.B.Primrose, 5th edition, 2006.


SEMESTER THREE

SUBJECT (SC) INDUSTRIAL MICROBIOLOGY

COURSE CODE

UNIT I

Introduction: History and Development, Different types of fermentation process. Inoculum

Preparation, isolation of microbes, fermentation media, industrial sterilization,serial subculture,

Microbial transformations.

UNIT II

Microbial Growth kinetics: Batch culture, continuous culture,Fed batch culture. Design of a

fermenter, types of fermenter- CSTF, Tubular, packed bed reactor, fluidized bed. Applications of

a fed batch reactor.

UNIT III

Parts and configuration of a fermenter, Downstream processing, Recovery processing-extraction

methods, and purification process.

UNIT IV

Bioprocess control and monitoring – temperature, pressure, agitation and pH. Computers in

bioprocess control.

UNIT V

Industrial process of ethanol, vinegar, amylase, protease, glutamic acids, lactic acids. Antibiotics

– streptomycin.

TEXT BOOKS:

1. Industrial Microbiology – L.E.Casida, , New Age International Pvt Ltd, 1st edition,1968.

REFERENCES:

1. Biochemical Engineering and Fundamentals – James E. Bailey & Ollis, Mc Graw Hill

Publishers, New york, 2nd edition, 2005.

2. Principles of Fermentation Technology – Stanbury & Whitaker Pergamon Press,

Oxford, 5th edition, 1996.


15BT501 - ENVIRONMENTAL BIOTECHNOLOGY

UNIT I 12 Role of biotechnology in environmental protection, bioremediation & applications, waste water treatment using

aquatic plants, root zone treatment, packed microbes, Genetically engineered microbes & applications, bio-

augmentation, bio-sorption & uses, immobilization-methods & applications

UNIT II 12 Waste water treatment- characteristics of waste water, microbiology of waste water biological treatment of waste

water, adsorption, chlorination, oxidation ponds, trickling filter, aerobic treatment (ASP), anaerobic treatment

(UASB) & role of microbes in waste water treatment, reuse of waste water

UNIT III 12 Air pollution control - bio-trickling filter, solid waste management - land fill, anaerobic digestion, pyrolysis,

incineration, restoration of degraded lands. Bioremediation of xenobiotics, Heavy metals pollution &

bioremediation, bio-miming-mechanism & applications

UNIT IV 12 Removal of specific pollutants-nitrogen & phosphorus, removal of oil & grease Biological treatment for food, paper

industries, SCP from waste. Novel methods for pollution control- vermicomposting, bioenergy from waste- biogas

production, bio-plastics.

UNIT V 12 Bio-pesticides – bacterial - Bt insecticide, viral, myco pesticide, Neem insecticide. Bio-fertilizers- rhizobial,

azotobacter, azospirillum, mycorhiza & advantages. Applications of bio-fertilizers & Bio-pesticides in pollution

control

Total Periods (60L+15T): 75

TEXT BOOK:

1. S.N. Jogdand, Environmental biotechnology (Industrial pollution management), Himalaya publication,

3rd

edition, 2004.

REFERENCE:

1. S.K. Agarwal, Environmental biotechnology, TBH Himalayas Publications, 2nd

edition, 1999.

2.Metcalf & Eddy, Franklin L. Burton, H. XDavid Stensel, Waste water engineering treatment and reuse,

Tata McGraw Hill publication, 2005, 4th edition.

Verified by Course Coordinator Approved by BOS Chairperson

(Dr.A.Dhivya ) (Dr.D.Jayasheela)

Semester V

Credit 5

Paper Type Core

Max.

Marks

CIA:25 +

CE :75


15BT502 - BASICS OF BIOPROCESS TECHNOLOGY

UNIT – I 9 Introduction to Biological processes: The range of fermentation processes – Microbial biomass, microbial enzymes,

microbial metabolites, recombinant products, transformation processes.

UNIT – II 9 Isolation of industrially important micro-organisms, Screening methods – Primary and Secondary screening.

Preservation of industrially important micro-organisms. Quality control of preserved stock cultures. Improvement

of industrial micro-organisms.

UNIT – III 9 Development of inocula for industrial fermentations – Yeast processes: Brewing, Baker’s yeast. Bacterial processes,

Mycelia processes: Sporulation on solidified media, in solid media and in submerged culture.

UNIT – IV 9 Design of a fermentor: Body construction, Construction materials, Aeration and agitation, aeration system.

Monitoring and control of various parameters. Control systems - Manual control, Automatic control: Two position

controllers (on/off), Proportional, Integral and Derivative control (PID). Computer applications in fermentation

technology.

UNIT – V 9 Downstream Processing: Recovery – Removal of microbial cells and other solid matter. Purification – Foam

separation, Precipitation, Filtration, Centrifugation. Physical, mechanical and chemical methods of Cell disruption,

Chromatography, Membrane processes, Drying and Crystallization


TEXT BOOKS:-

1.P.F. Stanbury, A. Whitaker and S.J. Hall, Principles of Fermentation Technology, Elsevier Science

Limited, 2nd

edition, 2005.

REFERENCES:-

1. A. H. Patel, Industrial Microbiology, Mac Millan India Ltd, 1st edition, 1984.

2. L. E. Casida JR., Industrial Microbiology, New Age International Pvt Ltd, 1st edition,1968.

3. Wulf Crueger and Anneliese Crueger, Industrial Microbiology, Panima Publishing Corporation,

Revised Edition, 2004.


(Dr.A.Dhivya ) (Dr.D.Jayasheela)

Semester V

Credit 4

Paper Type Elective

Max.

Marks

CIA: 25 +

CE :75


15BT502A - BIOLOGICAL CHEMISTRY

UNIT I 9 Behavioral Basis of Biological Organization. The dynamic expression of cell Organization. Flow of mass, Flow of

energy. Chemical Work, Transport, Electric work. Mechanical work, Regulatory work, other special forms of work.

UNIT II 9 Kinetics of cell growth, Cell Cycle, Cell reproduction, Cell Cycle parameters, Analysis of the cell cycle, events in

the cell cycle, G, S, G2, Cell division, Cytokinesis control of cell cycle, Dividing and Non dividing cells, Generation

time, Synchronization of growth, Cell Transformation and Malignant tumor growth, Circulation and life time of

cells in body.

UNIT III 9 Cell Adhesion, Cell signaling, Endocrine, Paracrine, Autocrine and synaptic transmission, Concept of receptors,

characterization and function, Extracellular and intracellular, Receptor ligand interaction, Concept of agonist and

antagonist.

UNIT IV 9 G- Proteins – Structure and function, Adenylate Cyclase system, cAMP – pK & cREB proteins, Signal Transduction

- Mechanisms of Signal transduction pathways in development and disease, Signal – transducing machinery as

targets for potential drugs.

UNIT V 9 Nature, Selective Permeability of Biomembrane, Selectivity and ion specificity of biomembrane, Role of Carriers in

ion transport (ex: Valinomycin and Gramicidin), Transporting ATPase – Na- K ATPase, Calcium ion transporting

ATPase of Sarcoplasmic reticulum, Transport of Macromolecules with and without vesiculation and by intermediate

mechanism.


TEXT BOOK:

1.De Robertis E.D.P and De Robertis E.M.P, Essentials of Cell and Molecular Biology, B.I Publications,

8th edition, 2007.

REFERENCE:

1. Sheelk P and Birch D. E., Cell Biology structure, Biochemistry and Function, John Wiley and Sons,

1st edition, 1983.

2. Spragg S.E., Physical Behaviour of macromolecules with biological functions, John Wiley and Sons,

1st edition, 1980.

3. A. Kotyk, K. Janacek and J. Koryta, Biophysical chemistry of membrane functions, John Wiley and

Sons, 1st edition, 1988.


(Dr.A.Dhivya) (Dr.D.Jayasheela)

Semester V

Credit 4

Paper Type Elective

Max.

Marks

CIA:25 +

CE :75


15BT502B – BIOPHYSICS

UNIT I 9 Acids and Bases , Mole and Normality, Weak acids, Amphoteric electrolytes, pH, Calculation of pH form H and OH

concentration, Measurement of pH, Henderson Hasselbalch equation, Titration Curve and pK Values, Buffers and

Stability of their pH

UNIT II 9 Structure of Atom, Bonds between atom and molecules – Ionic, Covalent, Hydrogen, Electrostatic, Disulphide and

Peptide bonds, vanderwaal’s forces, Bond energies, Bond angles, Weak interactions

UNIT III 9 Amino acid, Nucleic acid bases and lipids, Classification and Properties of Amino acid, Peptides and Polypeptides,

Nucleosides, Nucleotides, Polynucleotides, Pentose and Hexose polysaccharides, Amino acid to Peptides,

Polypeptides, Different types of linkages

UNIT IV 9 Structural level of proteins and stabilizing forces, conformational properties of Polypeptides – Ramachandran plot,

Helical Parameters and conformation, Organization and interaction of angles, Conformational structure of alpha

keratin, Silk Fibrin, Collagen, Folded conformation of globular proteins – Hemoglobin and myoglobin.

Classification and role of Beta bends and bulges, Super secondary structure, Domain and motifs

UNIT V 9 Double helical structure of DNA, Conformational Parameters of Nucleic acids and their constituents, Chargaff’s

rule, DNA Polymorphism, DNA Supercoiling, Hyperchromicity, Circular DNA, Types and Structure of RNA,

mRNA, rRNA, tRNA and modified nucleotides


TEXT BOOKS:

1. P. Narayanan, Essentials of Biophysics, New age international Publishers, 1st edition, 2000.

2. Jeremy M.Berg, Lubert Stryer, Biochemistry, W.H. Freeman and Company, New York, 5th edition,

2002.

REFERENCES:

1. Vasantha pattabhi, N. Gautham, Biophysics, Narosa Publishing House Pvt Ltd, 1st edition, 2002.

2. Thomas E. Creighton, Proteins – Structure and molecular properties, W.H. Freeman and Company,

New York, 5th edition, 1984.



Semester V

Credit 4

Paper Type Elective

Max. Marks CIA:25 +

CE :75


15BT503 – ENZYMOLOGY

UNIT I 7 Enzyme: Classification and Nomenclature, Structure of Enzyme – Active and catalytic site, General properties of

Enzymes, Isoenzymes.

UNIT II 7 Enzyme catalysis: Acid –base, Covalent, metal ion. Extraction and purification of enzymes – Physical and chemical

methods.

UNIT III 7 Enzyme Kinetics – Effect of pH and temperature in enzyme activity. Enzyme Inhibitors: Competitive, Non-

competitive and Uncompetitive inhibitors. Allosteric interaction of enzymes.

UNIT IV 7 Immobilization of Enzymes: Entrapment – Gel and fiber: Micro encapsulation, Phase separation, Covalent binding,

Cross linking, Physical adsorption. Applications of immobilized enzymes.

UNIT V 7 General aspects of Enzyme production – Semisolid and submerged culture methods; Enzymes as fermentation

products amylases, proteases, Pectinases, invertases and other enzymes.


TEXT BOOK:

1. Enzyme biotechnology Hand book, Asia Pacific Business Press, 6th edition, 2004.

2. Voet and Voet, Fundamentals of Biochemistry, John Wiley and Sons, 4th edition, 2005.

3. A.H. Patel, Industrial Microbiology, Mac Millan India Ltd, 1st edition, 1984.

REFERENCE: 1. Lehninger, Nelson, Cox, Principles of Biochemistry, CBS Publishers, 4

th edition, 1998.

2. L.E.Casida JR., Industrial microbiology , New Age International Pvt Ltd, 1st edition, 1968.



Semester V

Credit 3

Paper Type Skill Based

Max. Marks CIA:25 +

CE :75


15BT504 - LAB IN ENVIRONMENTAL BIOTECHNOLOGY

1. Isolation & enumeration of microbes from waste water

2. Batch culture

3. Estimation of total alkalinity, carbonate & bicarbonate

4. Estimation of dissolved oxygen

5. Estimation of chloride

6. Estimation of TDS & TSS

7. Ethanol Production

8. Composting

9. Mushroom Cultivation

10. Spirullina Production

Total Periods: 45

REFERENCE BOOK: 1. Dr. S. Rajan and Mrs. R. Selvi Christy, Experimental Procedures in Life Sciences, Anjana Book

House, 1st edition, 2010.



Semester V

Credit 3

Paper

Type

Practical

Max.

Marks

CIA:40 +

CE :60


15BT505 - LAB IN INDUSTRIAL BIOTECHNOLOGY

1. Assay of amylase activity

2. Determination of specific activity of amylase

3. Assay of protease activity

4. Microbial production of protease enzyme

5. Effect of PH on any one enzyme activity

6. Effect of temperature on any one enzyme activity

7. Media optimization of microbial fermentation(enzyme)

8. Analysis of fermentation products

Total Periods: 45

REFERENCE BOOKS: 1. Dr. S. Rajan and Mrs. R. Selvi Christy, Experimental Procedures in Life Sciences, Anjana Book




Semester V

Credit 3

Paper Type Practical

Max.

Marks

CIA:40 +

CE :60


15BT601 - PLANT BIOTECHNOLOGY AND ANIMAL

BIOTECHNOLOGY

UNIT I 12 Historical events in plant tissue culture, Plant tissue culture, Callus induction, organogenesis, Meristem culture,

anther, pollen, embryo culture and their application.

UNIT II 12 Protoplast technology - protoplast isolation, protoplast fusion and its application. Applications of plant tissue

culture: - Elimination of pathogens, Germplasm conservation, somoclonal variation, Embryo rescue, Artificial seeds,

Production of secondary metabolites, production of somatic hybrids.

UNIT III 12 Agrobacterium mediated gene transfer, Plant viral vectors. Resistance to herbicide, insecticide, virus and bacteria.

Plant tissue culture techniques for crop improvement in dicots and monocots

UNIT – IV 12 Animal cell and Tissue culture: Culture media Primary culture, sub-culture and establishment of cell lines, types of

cell lines, maintenance of cell lines, cloning of cell lines. Collection and Preservation of embryos.

UNIT – V 12 Molecular techniques in cell culture – In situ Molecular Hybridization, Somatic cell fusion, Production of

Monoclonal antibodies. DNA transfer – Co precipitation with CaCl2, Lipofection, Electroporation, Other methods.

Transgenics: Transgenic animals – Production and application, Expression of the bovine growth hormone;

transgenic in industry – vaccine production.


TEXT BOOK:

1. R. Ian Freshney, Culture of Animal cells, A manual of basic technique, John Wiley and Sons, 5th

edition, 2005.

2. M.M. Ranga, Animal Biotechnology, Agrobios Publications, 2nd

edition, 2004.

3.M.K. Razdan, An Introduction to plant tissue culture, Oxford and IBH Publishing Company, New

Delhi, 2000.

REFERENCES:

1. Slater et al., Plant Biotechnology: The Genetic Manipulation of Plants Oxford University Press,

Oxford, 3rd

edition, 2003.

2.Mantell S.H and Smith H, Plant Biotechnology, Cambridge University Press, UK, 1st edition,1983.

3. P.K. Gupta, Elements of Biotechnology, Rastogi Publications, 1st

edition, 1997.

4. B.D. Singh, Biotechnology, Kalyani Publications, 2nd

edition, 2003.



Semester VI

Credit 5

Paper Type Core

Max. Marks CIA:25 +

CE :75


15BT602 - GENOMICS AND PROTEOMICS

UNIT – I 9 Organization and Structure of Genomes – Viruses and Bacteria, Organelle DNA, Chloroplast DNA, Mitochondrial

genome. Organization of nuclear DNA in eukaryotes. Human Genome Projects – Mapping, Sequencing and the

future of human genome project.

UNIT – II 9 Genomics: Types of genomics – Structural and Functional genomics. Sequencing genes and short stretches of DNA:

Sanger-Coulson method, Maxam-Gilbert method and Automated Sequencing. Studying whole genomes – Pulsed

Field Gel Electrophoresis (PFGE).

UNIT – III 9 Mapping genomes: Genetic mapping – DNA markers for genetic mapping, Linkage analysis. Physical mapping –

Restriction mapping, Fluorescent insitu hybridization (FISH), RFLP and AFLP and Sequence tagged site mapping

(STS).

UNIT – IV 9 Proteomics: Types of proteomics – Expression, Structural and Functional proteomics. Analyzing gene expression:

DNA micro arrays – design, analysis and visualization of data, Two -dimensional gel electrophoresis (2-DE)

UNIT – V 9 Comparative genomics – comparative genomes of bacteria, organelles – mitochondrial and nuclear DNA and

eukaryotes – genes and regulatory elements. Comparative genomics in the study of human disease genes.


TEXT BOOKS:

1. S.B.Primrose and R.M.Twymann, Principles of gene manipulation and genomics, Blackwell

Publishing, 5th edition, 1994.

2. James. D. Watson, Michael Gilman, Recombinant DNA, W. H. Freeman and Company, New York,

2nd

edition, 1990.

REFERENCES:

1. T.A.Brown, Genomics, Garland Science Publishers, 2nd

edition, 2002.

2. T.A. Brown, Genetics – A Molecular Approach, Garland Science Publishers, 3rd

edition, 2004.

3. R. C. Dubey, A Text book of Biotechnology, S.Chand and Company Ltd, 2nd

edition, 1993.



Semester VI

Credit 3

Paper Type Skill Based

Max. Marks CIA:25 +

CE :75


15BT603 - LAB IN PLANT BIOTECHNOLOGY AND ANIMAL

BIOTECHNOLOGY

1. PTC Laboratory organization

2. Sterilization Procedures & Preparation of medium

3. In vitro germination of seeds

4. Embryo culture

5. Callus induction

6. Regeneration of shoots, roots from callus culture

7. Suspension culture

8. Protoplast isolation – mechanical method

9. Artificial seed production

10. Preparation & sterilization of ATC medium (Demo)

11. Preparation of primary culture from liver /muscle tissue (rat)

12. Isolation and Microscopic observation of monolayer cells

Total Periods: 45





Semester VI

Credit 3


Max.

Marks

CIA:40 +

CE :60


15BT604 - LAB IN CLINICAL BIOCHEMISTRY

URINE ANALYSIS:

1. Estimation of Creatine and Creatinine – Jaffe’s method

2. Estimation of Calcium – Clark and Collip’s method

3. Estimation of Phosphorus – Fiske and Subbarow’s Method

4. Estimation of Glucose

BLOOD ANALYSIS:

1. Estimation of Glucose – Glucose Oxidase Method

2. Estimation of Protein – Biuret Method

3. Estimation of Phospholipid

4. Estimation of Urea

5. Estimation of Uric acid

6. Estimation of SGOT and SGPT

7. Estimation of Free and Total Cholesterol

Total Periods: 45





Semester VI

Credit 3


Max. Marks CIA:40 +

CE :60


SC - CLINICAL BIOTECHNOLOGY

UNIT I 9 Disorders of carbohydrates metabolism: Normal sugar level in blood. Renal threshold and regulation of blood

glucose concentration. Hyperglycemia and Hypoglycemia. Diabetes Mellitus – introduction and Types of Diabetes

Mellitus. Glycosuria. Urine testing – Random blood Sugar and GTT (Glucose tolerance test).

UNIT II 9 Disorders of Lipid metabolism: Introduction to Plasma lipids and lipoproteins. Hyper lipoproteinemia and

Hypolipoproteinemia. Fatty liver. Hyperlipidemia and Hypercholoesterolemia and Hypocholestrolemia.

UNIT III 9 Diseases of aminoacid metabolism: plasma proteins abnormalities. Total plasma protein, Abnormal non - protein

nitrogen – urea, uric acid, creatinine, ammonia. Amino acid metabolism – cystinuria, phenyl ketonuria, maple syrup

disease, alkaptonuria, albinism and hartnup disease

UNIT IV 9 Classification of Pathogenic Microbes, Gram negative and Gram Positive, Spirochates. The General Principles of

Microbiology Laboratory – Sputum, Blood culture. Anti- microbial Chemotherapy- Gentamycin, cephalosporin,

Antibiotic resistance.

UNIT V 9 Mycobacterium Tuberculosis, Hepatitis C virus, Applications and Techniques – CRP, ASO, RA, ELISA, WIDAL


TEXT BOOK:

1.MN Chatterjea, Rana Shinde, Text Book of Medical Biochemistry, Jaypee Brothers medical Publishers,

6th edition, 2005.

REFERENCE:

1. D.C. Shanson, Microbiology in clinical Practise, Wright Publishers, 2nd

edition, 1989.

2. Frederick .W. Alt, Advances in Immunology volume 91, Academic Press Publications, 1st edition,

2006.



Semester VI

Credit 4

Paper

Type

Supportive

Course

Max.

Marks

CIA:25 +

CE :75


SC - HERBAL TECHNOLOGY

UNIT I 9 General Introduction: Definition, source of herbal raw materials, identification, authentication, standardization of

medicinal plants as per WHO guidelines and different herbal pharmacopoeias. Collection and processing of herbal

drugs. Seasonal and geographical variations; natural and artificial drying methods. Packaging and labeling of herbal

drugs prior to extraction

UNIT II 9 Standardisation: Determination of Physical and chemical constants such as extractive values, moisture content,

volatile oil content, ash values, bitterness value and foreign matters applicable to the various herbal drugs

UNIT III 9 Nutraceuticals: Pesticides from natural origin, Natural allergens and Other toxic plants. Herbal formulations :

Principle, methods, single herb formulation, Poly- formulation and their merits and demerits. Standardisation of

various herbal formulations.

UNIT IV 9 Plant tissue culture techniques and its application in pharmacy: Introduction, techniques of initiation and

maintenance of various types of cultures. Immobilized cell techniques and biotransformation studies including

recent developments in production of biological active constituents in static, suspension and hairy root cultures.

UNIT V 9 Analysis of bioactive Components of Natural sources: Phyto-chemical standardization of raw herbal extracts and

their formulation by using TLC, HPTLC, GC, HPLC, UV & IR Techniques


TEXT BOOK:

1. S.S.Purohit, Biotechnology, Agro bios Publication, Revised Edition, 2002.

REFERENCE:

1. S.S. Agarwal and M.Paridhavi, Herbal Drug technology, Universities Press, 2nd

edition, 2007.

2. Pulok K. Mukherjee, Quality Control of Herbal Drugs: An approach to evaluation of botanicals,

Business Horizons, 3rd

edition, 2002.

3. C.K.Kokate, A.P. Purohit and S.B. Gokhale, Nirali Prakashan, Pharmacognosy, Revised Edition, 29th

edition, 2009.



Semester VI

Credit 4

Paper Type Supportive Course

Max. Marks CIA:25 +

CE :75


SC- MEDICAL BIOCHEMISTRY

UNIT I 9 Clinical importance of enzyme: Serum enzymes- in Heart, Liver, Muscle and Bone diseases. Radioisotopes: In

medicine, diagnostic and therapeutic uses.

UNIT II 9 Renal functional test: Glomerular filtration, renal blood flow and tubular function. Liver function test: changes in

plasma protein test based on excretory liver function, abnormalities of bile pigment metabolism and lipid

metabolism.

UNIT III 9 Gastric function test: Fractional gastric analysis – using test meals, Stimulation tests and tubeless gastric analysis.

Thyroid function test: Thyroid scanning and immunological test for thyroid functions.

UNIT IV 9 Distribution of water and electrolytes in the body- Normal and abnormal balance of water and electrolytes and their

pathological variations. Acid- Base balance: Role of different buffer system, acid base regulation, balance and

imbalance.

UNIT V 9 Cerebrospinal fluid: Clinical significance- Appearance, pressure, biochemical changes and other chemical

constituents. Urine analysis: chemical significance- physical examination, organic and inorganic constituents, their

abnormal constituents.


TEXT BOOKS:

1. MN Chatterjea, Rana Shinde, Text book of medical biochemistry, Jaypee brothers medical

publishers, 6th edition, 2005.

REFERENCE:

1. D.C. Shanson, Microbiology in clinical Practise, Wright Publishers, 2nd

edition, 1989.

2. Frederick .W. Alt, Advances in Immunology volume 91, Academic Press Publications,

1st edition, 2006.



Semester VI

Credit 4

Paper Type Supportive

Course

Max. Marks CIA:25 +

CE :75