The Lighter Side Song: Enzymes (To the tune of ‘‘Downtown’’)* h S Received for publication, December 29, 2008, and in revised form, January 29, 2009 Kevin Ahern‡ From the Department of Biochemistry and Biophysics, Oregon State University, Corvalis, OR Reactions alone Could starve your cells to the bone Thank God we all produce Enzymes Units arrange To make the chemicals change Because you always use Enzymes Sometimes mechanisms run like they are at the races Witness the Kcat of the carbonic anhydrases How do they work? Inside of the active site It just grabs onto a substrate and squeezes it tight In an ENZYME! CAT-al-y-sis In an ENZYME! V versus S In an ENZYME! All of this working for you Energy peaks Are what an enzyme defeats In its catalysis Enzymes Transition state Is what an enzyme does great And you should all know this Enzymes Catalytic action won’t run wild - don’t get hysteric Cells can throttle pathways with an enzyme allosteric You know it’s true So when an effector fits It will just rearrange all the sub-u-nits Inside an ENZYME! Flipping from R to T ENZYME! Slow catalytically ENZYME! No change in Delta G You should relax When seeking out the Vmax though There are many steps Enzymes Lineweaver Burk Can save a scientist work With just two intercepts Enzymes Plotting all the data from kinetic exploration Lets you match a line into a best fitting equation Here’s what you do Both axes are inverted then You can determine Vmax and Establish Km for your ENZYMES! Sterically holding tight ENZYMES! Substrates positioned right ENZYMES! Inside the active site Enzymes * Copyright Ó 2009 Kevin Ahern h S This article contains an MP3 audio file available in the online version of this article. It can also be found at http://www. davincipress.com/metabmelodies.htm ‡To whom correspondence should be addressed. Department of Biochemistry and Biophysics, Oregon State University, Corva- lis, OR 97331. Tel.: 541-737-2305. E-mail: [email protected]. DOI 10.1002/bmb.20276 This paper is available on line at http://www.bambed.org 260 Q 2009 by The International Union of Biochemistry and Molecular Biology BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION Vol. 37, No. 4, p. 260, 2009

Song: Enzymes (to the tune of “Downtown”)

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Page 1: Song: Enzymes (to the tune of “Downtown”)

The Lighter Side

Song: Enzymes (To the tune of ‘‘Downtown’’)*hS

Received for publication, December 29, 2008, and in revised form, January 29, 2009

Kevin Ahern‡

From the Department of Biochemistry and Biophysics, Oregon State University, Corvalis, OR

Reactions aloneCould starve your cells to the boneThank God we all produceEnzymes

Units arrangeTo make the chemicals changeBecause you always useEnzymes

Sometimes mechanisms run like they are at the racesWitness the Kcat of the carbonic anhydrases

How do they work?

Inside of the active siteIt just grabs onto a substrateand squeezes it tightIn anENZYME!CAT-al-y-sisIn anENZYME!V versus SIn anENZYME!All of this working for you

Energy peaksAre what an enzyme defeatsIn its catalysisEnzymes

Transition stateIs what an enzyme does greatAnd you should all know thisEnzymes

Catalytic action won’t run wild - don’t get hystericCells can throttle pathways with an enzyme allosteric

You know it’s true

So when an effector fitsIt will just rearrangeall the sub-u-nitsInside anENZYME!Flipping from R to TENZYME!Slow catalyticallyENZYME!No change in Delta G

You should relaxWhen seeking out the Vmax thoughThere are many stepsEnzymes

Lineweaver BurkCan save a scientist workWith just two interceptsEnzymes

Plotting all the data from kinetic explorationLets you match a line into a best fitting equation

Here’s what you do

Both axes are inverted thenYou can determine Vmax andEstablish Kmfor yourENZYMES!Sterically holding tightENZYMES!Substrates positioned rightENZYMES!Inside the active siteEnzymes

*Copyright �2009 Kevin AhernhS This article contains an MP3 audio file available in the online

version of this article. It can also be found at http://www.davincipress.com/metabmelodies.htm

‡To whom correspondence should be addressed. Departmentof Biochemistry and Biophysics, Oregon State University, Corva-lis, OR 97331. Tel.: 541-737-2305. E-mail: [email protected].

DOI 10.1002/bmb.20276 This paper is available on line at http://www.bambed.org260

Q 2009 by The International Union of Biochemistry and Molecular Biology BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION

Vol. 37, No. 4, p. 260, 2009