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SPATIAL OMICS WITHOUT COMPROMISE Sensitivity, scale and speed
To understand biology, you need to understand cells. And to truly understand cells you need to understand their positional context across time and space. You need to analyze individual cells in their native environments at high throughput to uncover the quantitative insights that will drive your research forward.
Rebus Biosystems™ has developed an automated omics solution that provides subcellular resolution of biological molecules directly in and across large tissue sections. Customizable assays allow you to focus on the targets that matter most to your research, whether that be in neuroscience, immunology, oncology, infectious disease, development, or any other area of biology.
Insights from the anatomical to the molecular
Focus on the targets that matter most to your research at subcellular resolution in hundreds of thousands of cells in a single experiment
The Rebus Biosystems spatial omics solution will be available as a fully integrated instrument for your lab in early 2021, but early access is now available through our Spatial Omics as a Service program.
Our Standard Service includes sectioning, sample preparation and analysis using our spatial omics solution and is available for fresh frozen samples from our growing list of validated tissue types in both mouse and human. A Custom Service option is available for tissues, sample types and species not yet validated on our platform.
Spatial Omics as a ServiceTake your research to the next level
CONTACT US TODAY
Learn more at rebusbio.com or email
us at [email protected] to discuss your project
SPATIAL TRANSCRIPTOMICSEvery transcript, in every cell
The first application developed for the Rebus Biosystems spatial omics platform, focused on spatial transcriptomics, leverages the sensitivity, scale and speed of the company’s patented Synthetic Aperture Optics system to enable high-throughput multiplex gene expression analysis with subcellular resolution.
Gold-standard single-molecule fluorescent in situ hybridization (smFISH) chemistry detects and quantifies individual transcripts in their original locations. Fully custom assays allow analysis of up to 30 genes.
Data is output as a spatially annotated CellxFeature matrix that can be used for unbiased clustering, cell type annotation and precise mapping.
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24 Gene Spatial Transcriptome of Mouse Brain
A single 10µm coronal section from a fresh frozen 10-week-old mouse brain was probed for a curated 24 gene panel for identification of all major brain cell types. Imaging and data processing were completed for this 50mm² section within two days.
With the Rebus Biosystems spatial omics solution you can:
Validate single-cell RNA sequencing results
Identify novel cell types/states and link tissue architecture to cellular function
Map dynamics of normal development or disease progression
Elucidate biomarkers and cellular interactions to drive drug discovery
Excitatory neurons
Rorb
Lamp5
Vasculature
Apln
Flt1
Immune and Astrocytes
Hexb
MfegB
Inhibitory neurons
Kcnip2
Cnr1
Gad2
Housekeeping genes
Polr2A
Polr2B
Top1
L1cam
Rab1a
Xrcc5
ActB
Oligodendrocytes
Tmem6
Itpr2
Pdgfra
Ctps
Sox10
Plp1
Bmp4Anln
MOUSE BRAIN 24 GENES
