Station 1: DNA StructureUse the figure above to answer each of the following questions.1.This is the subunit that DNA is composed of.2.This subunit is composed of what 3 parts?3.What molecules make up the sides of DNA?4.Who are the 2 scientists credited with discovering the

shape of the DNA molecule?5.If this molecule is composed of 28% Adenine, list the

percent composition of each of the 3 remaining bases.6. Define Chargaff’s Rule:

1.

Station 2: DNA ReplicationDraw DNA Replication. Put the lead strand on top and the lag strand on the bottom.Label the parent strands 5’ and 3’ ends. Label the new strands 5’ and 3’ ends.Use small summaries to explain what is going on during replication.Label the picture with these terms:DNA HelicaseDNA PolymeraseRNA PrimaseRNA PrimerOkazaki FragmentsLigase

Station 3: Gene Expression

1. The process of constructing a molecule of RNA from a segment of DNA is called what?

2. This takes place where in the cell?3. The process of constructing an amino acid chain from an

RNA molecule is called what?4. This takes place where in the cell?

2.

3.

1.

4.

Station 4: DNA & RNA1. List the 3 main

differences between DNA & RNA.

Station 5: Transcription

1. Which of the following would represent the strand of DNA from which the mRNA strand CCAUCGAUCGAC was made?

a. GGUAGCUAGCUG c. CAGCUAGCUACCb. CCATCGATCGAG d. GGTAGCTAGCTG

2. What enzyme breaks the hydrogen bonds and opens the DNA molecule?

3. What enzyme places the complementary RNA nucleotides in the correct place?

Station 6: TranslationRefer to the illustration below. What is the portion of the protein molecule coded for by a piece of DNA with the sequence CATCTAGGGGTA?

Refer to the illustration above. Suppose that you are given a protein containing the following sequence of amino acids: tyrosine, proline, aspartic acid, isoleucine, and cysteine. Use the portion of the genetic code given to determine which of the following contains the mRNA sequence that codes for this amino acid sequence.

a. AUGGGUCUAUAUACG c. GCAAACTCGCGCGTAb. ATGGGTCTATATACG d. UACCCAGAUAUAUGC

a. Ser—Tyr—Arg—Gly c. Leu—Lys—Cys—Pheb. Val—Asp—Pro—His d. Pro—Glu—Leu—Val

mRNA codons amino acidUAU, UAC tyrosineCCU, CCC, CCA, CCG prolineGAU, GAC aspartic acidAUU, AUC, AUA isoleucineUGU, UGC cysteine

Station 7: Translation

1. This is the organelle within which translation is taking place.2. Which amino acid is this?3. What is this molecule?4. What will the anticodon be on the next tRNA molecule that

transfers an amino acid to this mRNA molecule?

1.

2.

3.

Station 8: TranslationDraw a picture illustrating how mRNA, rRNA, and tRNA all work together to produce proteins. Make sure that you write neat enough that I can identify the codons(yes, I need to see the nitrogen bases), the anticodons, and the amino acids in the protein chain (use the codon chart, the bottom of this page, to show which amino acid goes with the codon).

Station 9: MutationsOriginal DNA Code: TACGGTCATTGGCCAAmino Acid Chain: Met-Pro-Val-Thr-Gly

1. If highlighted G is replaced by a T, what would be the resulting amino acid chain?

2. What type of mutation is this?

Original DNA Code: TACGGTCATTGGCCAAmino Acid Chain: Met-Pro-Val-Thr-Gly

3. What would the amino acid chain be if an A is placed between the highlighted C and G?

4. What type of mutation is this?

Station 10: DNA Regulation

1. What part Gene expression is this image showing?2. This gene segments show the Lac operon,

a. Which of these sections get transcribed?b. What is B representing?c. What sits at C to keep the process from turning on when it is not

needed?d. What molecule has to enter the system for this gene to get turned

on?e. Why do genes need to be regulated (why can’t they be turned on

all the time?

3. Which part of the mRNA gets cut out before translation?4. Which parts are spliced together and translated into a protein

chain?

Station 1: DNA Structure

Use the figure above to answer each of the following questions.1. This is the subunit that DNA is composed of. (nucleotide)2. This subunit is composed of what 3 parts? (deoxyribose sugar, phosphate, nitrogen base)3. The sides are made of repeating phosphates and deoxyribose sugars4. Who are the 2 scientists credited with discovering the shape of the DNA molecule? (Watson & Crick)5. If this molecule is composed of 28% Adenine, list the percent composition of each of the 3 remaining

bases. (28% Thymine, 22% Cytosine, 22% Guanine)6. Chargaff’s Rule is that A and T always bond together and C and G always bond together

Station 2: DNA ReplicationDraw DNA Replication. Put the lead strand on top and the lag strand on the bottom.Label the parent strands 5’ and 3’ ends. Label the new strands 5’ and 3’ ends.Use small summaries to explain what is going on during replication.Label the picture with these terms:DNA HelicaseDNA PolymeraseRNA PrimaseRNA PrimerOkazaki FragmentsLigase

Station 3: Gene Expression

1. The process of constructing a molecule of RNA from a segment of DNA is called what? (transcription)2. This takes place where in the cell? (nucleus)3. The process of constructing an amino acid chain from an RNA molecule is called what? (translation)4. This takes place where in the cell? (cytoplasm)

Station 4: DNA & RNA

1. List the 3 main differences between DNA & RNA.

DNA is double stranded while RNA is single stranded.DNA has thymine and RNA has uracil.DNA has deoxyribose sugar & RNA has ribose sugar.

Station 5: Transcription

1. Which of the following would represent the strand of DNA from which the mRNA strand CCAUCGAUCGAC was made?

a. GGUAGCUAGCUG c. CAGCUAGCUACCb. CCATCGATCGAG d. GGTAGCTAGCTG

2. What enzyme breaks the hydrogen bonds and opens the DNA molecule? (DNA helicase)3. What enzyme places the complementary RNA nucleotides in the correct place? (RNA polymerase)

Station 6: Translation

Refer to the illustration below. What is the portion of the protein molecule coded for by a piece of DNA with the sequence CATCTAGGGGTA?

Refer to the illustration above. Suppose that you are given a protein containing the following sequence of amino acids: tyrosine, proline, aspartic acid, isoleucine, and cysteine. Use the portion of the genetic code given to determine which of the following contains the mRNA sequence that codes for this amino acid sequence.

a. AUGGGUCUAUAUACG c. GCAAACTCGCGCGTAb. ATGGGTCTATATACG d. UACCCAGAUAUAUGC

Station 7: Translation

1. This is the organelle within which translation is taking place. (ribosome)2. Which amino acid is this? (Met)3. What is this molecule? (tRNA)4. What will the anticodon be on the next tRNA molecule that transfers an amino acid to this mRNA

molecule? (ACA)

Station 8: Translation

Draw a picture illustrating how mRNA, rRNA, and tRNA all work together to produce proteins. Make sure that you write neat enough that I can identify the codons(yes, I need to see the nitrogen bases), the anticodons, and the amino acids in the protein chain (use the codon chart, the bottom of this page, to show which amino acid goes with the codon).

Station 9: Mutations

Original DNA Code: TACGGTCATTGGCCAAmino Acid Chain: Met-Pro-Val-Thr-Gly

1. If highlighted G is replaced by a T, what would be the resulting amino acid chain?Met-His-Val-Thr-Gly

2. What type of mutation is this?Missense

Original DNA Code: TACGGTCATTGGCCAAmino Acid Chain: Met-Pro-Val-Thr-Gly

3. What would the amino acid chain be if an A is placed between the highlighted C and G?TACAGGTCATTGGCCA AUG UCC AGU AAC CGG U Met-Ser- Ser- Asn-Arg

4. What type of mutation is this?Frameshift - insertion

Station 10: DNA Regulation

1. What part Gene expression is this image showing?2. This gene segments show the Lac operon,

a. Which of these sections get transcribed? Db. What is B representing? RNA Polymerasec. What sits at C to keep the process from turning on when it is not needed? Repressord. What molecule has to enter the system for this gene to get turned on? Lactosee. Why do genes need to be regulated (why can’t they be turned on all the time? So that we only produce

what we need.

3. Which part of the mRNA gets cut out before translation? Introns (interfering introns)4. Which parts are spliced together and translated into a protein chain? Exons (expressed exons)