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PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
INTRODUCTION 2
What is a Digital Fluorescence Microscope?
• Unlike traditional microscopes, which utilize an eyepiece for performing visual inspection,
digital video microscopes utilize cameras to record and capture images, providing higher
resolution and higher precision than what is typically seen with the human eye. Since
infinity-corrected digital video microscopes can be quite complex, using the modular
TECHSPEC Optical Cage System is ideal for simplifying the assembly. In this case
another aspect has been added to the design to incorporate a fluorescence aspect to the
digital microscope. Fluorescence microscopy is ideal for measuring and analyzing the
absorption and excitation of various wavelengths of light. An in-line fluorescence
microscopy setup utilizes a dichroic filter to redirect light from an illuminator into the
perpendicular optical path.
Why TECHSPEC Optical Cage System?
• Building a customized digital fluorescence microscope with TECHSPEC Optical Cage
components allows the user to build a microscope quickly and easily with limitless
customization options. Objectives can be changed to modify system magnification, and
interchangeable filter holders make swapping filters easy. Custom illumination can be
added for bright field, dark field, or in-line illumination options. Additionally, a
spectrometer could be added for precise spectral measurements.
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
OVERVIEW 3
Complete Parts List
Filter Selection
Sub Assemblies:
1. Dichroic
Assembly
2. Objective Arm
3. Source Arm
4. Imaging Arm
Mounting & Alignment
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
COMPLETE PARTS LIST 4
STOCK # Description QUANTITY 85713 25.4mm Dia. Adapter for 85710 1
85710 Square Tip/Tile Optic Mount 1
Depends on Application 25mm Dia. Dichroic Filter 1
85624 Cage Sphere with (5) 30mm Port and (1) 43mm Port 1
85632 25mm Standard Cage Plate with M6 for Post Mounting 3
38944 0.316” ID, Fiber Optic Adapter SX-6 2
56371/56372
115V AC 50/60HZ, MH-100 Illuminator/220V AC 50/60HZ, MH-100
Illuminator 1
42346 1/4" x 24”, Flexible Fiber Optic Light Guide 1
85588 30mm Cage 25/25.4mm Diameter Thick Lens Mount 1
85737 30mm Square Interface Plate 5
85719 30mm Cage Plate C-Mount Adapter 3
55743 Mitutoyo to C-Mount 10mm Adapter 1
46143 5X Mitutoyo Plan Apo Infinity-Corrected Long WD Objective 1
85488 Cage Support Rod 6mm Diameter x 100mm Length, M3 4
85486 Cage Support Rod 6mm Diameter x 50mm Length, M3 2
85490 Cage Support Rod 6mm Diameter X 150mm 4
85630 30mm Standard Cage Plate with M6(1 for Post Mounting) 3
86018 Filter Holder Plate 2
85649 Interchangeable Filter Holder 2
Depends on Application Fluorescence Bandpass Filters (see details) 2
54774 MT-1 Accessory Tube Lens 1
58329 Mitutoyo MT-1/MT-2 C-Mount Adapter 1
85492 Cage Support Rod 6mm Diameter x 300mm Length, M3 4
54633 C-Mount Extension Tube (100mm Length) 1
54632 C-Mount Extension Tube (50mm Length) 1
03625 C-Mount Fine Thread Extension Tube (30mm-50mm Length) 1
65553 25mm Dia. 25mm Efl. Achromatic Lens 1
66014 25mm Dia. 20mm Efl. Aspheric Lens 1
59367 EO-3112C ½” CMOS Color USB Camera 1
59382 5m USB2.0 (mini-B to standard-A) Cable 1
85618 Micrometer Mount Kit 1
58661 Micrometer Head Anvil 13mm 1
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
OPTICAL FILTERS 5
A basic fluorescence microscope requires 3
filters. Each application will use a different
combination depending on the system
requirements:
• Excitation Filter: placed within the
illumination path of a fluorescence
microscope. It filters out all wavelengths of the
light source except for the excitation range of
the fluorophore or specimen under inspection.
• Dichroic Filter: placed between the excitation
filter and emission filter at a 45° angle. It
reflects the excitation signal towards the
fluorophore under inspection and transmits the
emission signal towards the detector.
• Emission Filter: placed within the imaging
path of a fluorescence microscope. It filters out
the entire excitation range of the fluorophore
under inspection and transmits the emission
range of the fluorophore.
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
1. DICHROIC ASSEMBLY
6
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
DICHROIC ASSEMBLY 7
STOCK # Description QUANTITY 85713 25.4mm Dia. Adapter for 85710 1
85710 Square Tip/Tile Optic Mount 1
N/A 25mm Dia. Dichroic of choice (see details under filter) 1
85624 Cage Sphere with (5) 30mm Port and (1) 43mm Port 1
85632 25mm Standard Cage Plate with M6 for Post Mounting 1
85737 30mm Square Interface Plate 1
86018 Filter Holder Plate 2
Parts needed for this step:
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
DICHROIC ASSEMBLY 8
1. Carefully insert the 25mm Dia. Dichroic
Filter into the 25.4mm Dia. Adapter (#85-
713). Lock into place with the M3 set
screws. Insert the adapter into the Square
Tip/Tilt Optic Mount (#85-710) and secure.
2. Attach the 25mm Standard Cage Plate
(#85-632) to the Cage Sphere (#85-624)
opposite the 43mm port with M3 SHCS.
Slide the base of the tip/tilt assembly from
step 1 into the sphere and secure within the
plate. (Alignment will be done later).
3. Attach two Filter Holder Plates (#86-018)
with M3 SHCS to two adjacent sides of the
sphere. Keep the the openings of the plates
facing the 43mm port. Attach a Square
Mounting Plate (#85-737) to a side opposite
of a filter holder plate.
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
2. OBJECTIVE ARM
9
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
OBJECTIVE ARM 10
STOCK # Description QUANTITY 85737 30mm Square Interface Plate 2
55743 Mitutoyo to C-Mount 10mm Adapter 1
46143 5X Mitutoyo Plan Apo Infinity-Corrected Long WD Objective 1
85719 30mm Cage Plate C-mount Adapter 1
85490 Cage Support Rod 6mm Diameter X 150mm 4
85488 Cage Support Rod 6mm Diameter x 100mm Length 2
85-618 Micrometer Mount Kit 1
58-661 Micrometer Head Anvil 13mm 1
Parts needed for this step:
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
OBJECTIVE ARM 11
4. Thread the C-Mount Adapter (#85-719), Mitutoyo
Adapter (#55-743), and Mitutoyo Objective (#46-
143) together. Then secure the C-Mount Adapter
part of the assembly within the square plate with
M3 set screws.
5. Insert the four 150mm long rods (#85-490) into
the outer rod holes of the square plate and
secure with M3 set screws.
6. Take a Square Mounting Plate (#85-737) and
slide it onto the 150mm long rods. Lock the plate
onto the rods approximately 20mm from the end
of the objective.
7. Insert two100mm long rods (#85-488) into two
adjacent inner rod holes of the square plate from
step 6. Keep the ends flush with the plate and
secure with M3 set screws.
8. Slide the 2 springs from the micrometer
assembly onto the 100mm long rods.
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
OBJECTIVE ARM 12
9. Attach the stainless steel plate (not shown) to a
Square Mounting Plate (#85-737) with M3 SHCS.
Slide the square plate onto the rods with the steel
plate between the100mm long rods and facing
away from the springs. Keep the plate loose on the
rods.
10. Mount the micrometer (#58-661) in the micrometer
holder. Slide the micrometer assembly onto the
two 150mm rods. The micrometer should be
between the two 100mm long rods Be sure the
micrometer is in the half extended position and the
shaft contacts the stainless steel plate from step 9.
11. Push the micrometer assembly and loose square
plate towards the secured square plate
compressing the springs to approximately ¾ their
uncompressed length. Lock the micrometer
assembly on the rods with M3 set screws keeping
the springs under tension. The loose square plate
should now translate when the micrometer is
adjusted. This loose square plate will serve as the
microscope stage.
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
3. SOURCE ARM
13
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
SOURCE ARM 14
STOCK # Description QUANTITY
85632 25mm Standard Cage Plate with M6 for Post Mounting 2
38944 0.316” ID, Fiber Optic Adapter SX-6 2
85588 30mm Cage 25/25.4mm Diameter Thick Lens Mount 1
85488 Cage Support Rod 6mm Diameter x 100mm Length, M3 2
85630 30mm Standard Cage Plate with M6 for Post Mounting 1
65553 25mm Dia. 25mm Efl. Achromatic Lens 1
66014 25mm Dia. 20mm Efl. Aspheric Lens 1
Parts needed for this step:
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
SOURCE ARM 15
12. Insert the 25mm Dia. x 25mm FL Achromatic Lens
(#65-553) into a 25mm Cage Mounting Plate.
Secure with M3 set screws.
13. Insert the 25mm Dia. x 20mm FL Plastic Aspheric
Lens (#66-014) into another 25mm Cage Mounting
Plate. Secure with M3 set screws.
14. Place one of the Fiber Adapters (#38-944) into the
25mm Thick Lens Mount (#85-588) and secure with
retaining ring. Insert the assembly into a 30mm
Cage Mounting Plate (#85-630). Secure with M3
set screws.
15. Insert two 100mm long rods onto the filter holder
next to the microscope objective. Secure with M3
set screws.
16. Slide the plates from steps 12, 13, and 14 onto the
rods in order of assembly: Achromatic Lens,
Aspheric Lens, then Fiber Adapter. Make sure the
more convex surfaces of the lenses facing the fiber
adapter.
17. Temporarily secure the plates on the rods for later
alignment.
Note: there is an extra plate shown in these images between the achromat and filter holder. This is optional for mounting purposes.
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
4. IMAGING ARM
16
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
IMAGING ARM 17
PART # Description QUANTITY 85737 30mm Square Interface Plate 2
85719 30mm Cage Plate C-Mount Adapter 2
85486 Cage Support Rod 6mm Diameter x 50mm Length, M3 2
54774 MT-1 Accessory Tube Lens 1
58329 Mitutoyo MT-1/MT-2 C-Mount Adapter 1
85492 Cage Support Rod 6mm Diameter x 300mm Length, M3 4
54633 C-Mount Extension Tube (100mm Length) 1
54632 C-Mount Extension Tube (50mm Length) 1
03625 C-Mount Fine Thread Extension Tube (30mm-50mm Length) 1
59367 EO-3112C ½” CMOS Color USB Camera 1
Parts needed for this step:
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
IMAGING ARM 18
13. Secure the MT-1 Tube Lens (#54-774)
within the Tube Lens C-Mount Adapter
Assembly (#58-329).
14. Thread a Cage C-Mount Adapter (#85-
719) onto the female threading of the
tube lens assembly. Insert the adapter
into the 30mm aperture of one of the
Square Plates (#85-737) and secure in
place with M3 set screws.
15. Thread the C-Mount Fine Thread
Extension Tube, 100mm C-mount
Extension Tube, and 50mm C-Mount
Extension Tube (#03-625, #54-632, #54-
633) onto the male threading of the tube
lens assembly.
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
IMAGING ARM 19
16. Thread the remaining C-Mount
Adapter (#85-719) onto the end of
the tube assembly. Slide a Square
Plate (#85-737) onto the C-mount
adapter.
17. Thread the C-Mount Camera (#59-
367) onto the C-Mount Adapter
after the square plate.
18. Insert all four 300mm Long Rods
(#85-492) through the rod holes in
the Square Plates. Keep the ends
flush with the plate connected to
the tube lens. Secure rods with M3
set screws.
19. Use two 50mm Long Rods (#85-
486) to connect the Square Plate
attached to tube lens to the Filter
Holder Plate opposite the objective
on the sphere. Secure rods with
M3 set screws.
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
MOUNTING & ALIGNMENT
20
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
MOUNTING 21
Adding two standard cage plates to the
microscope assembly make it easy to mount
this microscope either horizontally or vertically:
1. Add a plate to the source arm between the
achromatic lens and the filter holder. The
second can be placed along the 50mm
rods between the filter holder and tube
lens. (make sure the M6 or ¼-20 mounting
holes are facing the same direction.
2. Attach mounting posts to the two new
plates and adjust the placement of the
plates to match the breadboard or bench
plate spacing.
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
ALIGNMENT 22
• Rotate the tip/tilt mount from step 1 so the dichroic filter reflects the light source toward
the camera (instead of toward the objective). Adjust the location of the achromatic lens
and aspheric lens until the fiber optic light guide is in focus. Lock the location of the plates
on the rods.
• Rotate the tip/tilt mount again so the dichroic filter reflects the source through the
objective onto the sample. Adjust the angle until the focused spot is in the center of the
sample area. Using the micrometer, find the smallest focused spot.
• Once the microscope is assembled and aligned, the excitation and emission filters can be
secured within two Interchangeable Filter Holders (#85-649). The Filter Holders slide into
the filter holder plates for inspection.