Streaking culture plates in bacteriology by Dr.T.V.Rao MD

8/17/2019 Streaking culture plates in bacteriology by Dr.T.V.Rao MD

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STREAKINGCULTURE PLATES

BACTERIOLOGY

Dr.T.V.Rao MD

5/7/16 Dr.T.V.Rao MD 1


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Think Before Inoc!a"io

•Before inoc!a"ion#i$%or"an" infor$a"ioni& 'ri""en on "he (o""o$of "he %!a"e c!o&e "o"he ri$

•)*a"e of inoc!a"ion

•+."e$%era"re of inc(a"ion

•,.*ra"ion of inc(a"ion

•-.$icroorani&$ inoc!a"e*

5/7/16 Dr.T.V.Rao MD


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/ha" i& &"reakin•The $o&" co$$on $e"ho* of inoc!a"in an aar %!a"e i&&"reakin.

•S"reak %!a"e&•)./i"h "hi& $e"ho*# a &$a!! a$on" of &a$%!e i& %!ace* on&i*e of "he aar %!a"e 0ei"her 'i"h a &'a(# or a& a *ro% fro$

inoc!a"in !oo% if "he &a$%!e i& a !i1i*2.•+.A &"eri!e !oo% 03a$e* n"i! re* ho"# "hen coo!e* (4 "ochaar a'a4 fro$ "he inoc!a"e* &a$%!e2 i& "hen &e* "o &%r"he (ac"eria o" in one *irec"ion fro$ "he ini"ia! &i"e ofinoc!a"ion. Thi& i& *one (4 $o5in "he !oo% fro$ &i*e "o &%a&&in "hroh "he ini"ia! &i"e



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/ha" i& &"reakin•The !oo% i& "hen &"eri!i&e* 0(4 3a$inaain an* "he 6r&" &"reak& are "hen&%rea* o" "he$&e!5e&.

•.Thi& i& re%ea"e* +7, "i$e $o5in

aron* "he aar %!a"e.•/ha" &ho!* ha%%en i& "ha" &in!e(ac"eria! ce!!& e" i&o!a"e* (4 "he&"reakin# an* 'hen "he %!a"e i&

inc(a"e*# for$in *i&cre"e co!onie&"ha" 'i!! ha5e &"ar"e* fro$ &" one5/7/16 Dr.T.V.Rao MD


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The essential step in inoculatculture plates

•There are &e5era!e&&en"ia!%reca"ion& "ha"

$&" (e "aken*rin inoc!a"ion%roce*re& "ocon"ro! "he

o%%or"ni"ie& for5/7/16 Dr.T.V.Rao MD


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Pro$%" ac"ion 'i"h

O%"i$a! "i!i"4

•O%era"ion&$&" no" (e

&"ar"e* n"a!!re1ire$enare 'i"hin

i$$e*ia"ereach an*$&" (e

co$%!e"e* 5/7/16 Dr.T.V.Rao MD


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E9%o&e "he inoc!$ in ""(e& an* con"ainer& fo

$ini$a! Ti$e• Inoc!$ i& ro'n in "e&""(e& an* $&" (e o%enfor "he $ini$$ a$on"of "i$e %o&&i(!e an*'hi!e "he4 are o%en a!!

'ork $&" (e *one c!o&e"o "he Bn&en (rner3a$e 'here air crren"&are *ra'n %'ar*&.



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The Neck of Te&" "(econ"ainin inoc!$ "o

hea"e* (rie34

•On (ein o%en"he neck of a "(e or (o""!e$&" (ei$$e*ia"e!4'ar$e* (4

3a$in &o "haan4 air $o5e$i& o"'ar*& an"he 5e&&e! he!

near a& %o&&i(5/7/16 Dr.T.V.Rao MD


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/ork 'i"h a(&o!"e &"eri!i•The %ar"& of &"e

%i%e""e& "ha" 'i%" in"o c!"re&"eri!e 5e&&e!& $no" (e "oche* a!!o'e* "o co$e

con"ac" 'i"h o"h&"eri!e &rfacec!o"hin# "he &"he 'orkin areo"&i*e of "e&"

"(e&;(o""!e&5/7/16 Dr.T.V.Rao MD


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/ork U&in a 'ire !oo•/ire !oo%& are &"eri!i&e* &in re*hea" in a Bn&en 3a$e (efore an*af"er &e. The4 $&" (e hea"e* "ore* ho" "o $ake &re "ha" an4con"a$ina"in (ac"eria! &%ore&are *e&"ro4e*. The han*!e of "he'ire !oo% i& he!* c!o&e "o "he "o%#

a& 4o 'o!* a %en# a" an an!e"ha" i& a!$o&" 5er"ica!. Thi& !ea5e&"he !i""!e 6ner free "o "ake ho!*of "he co""on 'oo! %!;&cre' ca%of a "e&" "(e;(o""!e



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on ra%i*hea"in 'i"h "he%o&&i(i!i"4 of re!ea&in&$a!! %ar"ic!e& of c!"rean* aero&o! for$a"ion



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?an*!in of "heInoc!a"in !oo%

• Po&i"ion "hehan*!e en* o"he 'ire in "h

!ih" (!e conof "he 3a$e.Thi& i& "he coarea of "he 3



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Perfec" "he ar" han*!i"he Inoc!a"in !oo%

• Dra' "he re&" of"he 'ire %'ar*&&!o'!4 % in"o

"he ho""e&"reion of "he3a$e#0i$$e*ia"e!4



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?ea" an* coo! "he Loo%

• En&re "he f!en"h of "he recei5e& a*e1hea"in.. A!!o' "o coo"hen &ei$$e*ia"e!4.



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?an*!e "he Inoc!a"in !oo

Do no" %" "he!oo% *o'n or 'a5ei" aron*..

Re7&"eri!i&e "he !oo%i$$e*ia"e!4 af"er&e.



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Be caref! &o$e "i$e& "he Loo% $a4 con"ain inoc!$ $&" re*ra' "he inoc

•If a !oo% *oe& no

an4 !i1i* "he !ono" $a*e a co$circ!e. To correc%ro(!e$# 6r&" e"ha" "he !oo% ha

&"eri!i&e* an* "hre&ha%e "he !ooforce%&. Do no" 4or 6ner& (ec"he %o&&i(i!i"4 o

%nc"rin "he &5/7/16 Dr.T.V.Rao MD


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/orkin 'i"h (ac"eran* 4ea&" S"reak %!a"

•The !oo% i& &e* for%re%arin a &"reak%!a"e. Thi& in5o!5e&"he %rore&&i5e

*i!"ion of aninoc!$ of (ac"eriaor 4ea&" o5er "he&rface of &o!i*i6e*aar $e*i$ in a5/7/16 Dr.T.V.Rao MD


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The ai$ of "he %roce*re"o o("ain &in!e i&o!a"e

%re co!onie&• Loo&en "he ca%of "he (o""!econ"ainin "he

inoc!$.?o!* "he

!oo% in "he rih"han*. .



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?an*!in $a""er& ine""in "he rih" inoc

Lif" "he (o""!e;"e&" "con"ainin "he inoc'i"h "he !ef" han*. Re$o5e "he ca%;co"'oo! %! of "he

(o""!e;"e&" "(e 'i"!i""!e 6ner of "he rhan*. .


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?an*!in !oo% an* Inoc!a"he $a"eria! $a""er&

•In&er" "he !oo% in"o"he c!"re (ro"han* 'i"h*ra'. A"a!! "i$e ho!* "he

!oo% a& &"i!! a&%o&&i(!e..



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Do no" Prac"ice "he 'a



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Prac"ice for Perfec"io• Re%!ace "heca%;co""on 'o

%! on "he(o""!e;"e&" "(&in "he !i""!e6ner. P!ace

(o""!e;"e&" "((ench. . Par"ia!if" "he !i* of "Pe"ri *i&hcon"ainin "he5/7/16 Dr.T.V.Rao MD


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Prac"ice # Prac"ice (e&" 'a4Perfec"ion

• ?o!* "he chare* !oo%%ara!!e! 'i"h "he&rface of "he aar@&$ear "he inoc!$

(ack'ar*& an*for'ar*& acro&& a &$a!!area of "he $e*i$0&ee &"reake* area .



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S"reak "he P!a"e& in *e6ne* Manner

• /i"h "he co!oo% &"reak "

%!a"e fro$ aacro&& "he&rface of "haar in "hree

for %ara!!e!!ine& . Make&re "ha" a&$a!! a$on

c!"re i&5/7/16 Dr.T.V.Rao MD


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S"reak "he P!a"e& in *e6ne* Manner

• Re$o5e "he !oo% c!o&e "he Pe"ri *i&


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Carr4 'i"h fr"herinoc!a"ion&

•Re$o5e "he !oo% an*c!o&e "he Pe"ri *i&h. .


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Co$%!e"e "he 'ork C!o&e "%e"ri *i&h

• Re$o5e "he !an* c!o&e "he *i&h.

%o&i"ion. Therare a!"erna"i5$e"ho*& for%re%arin a

&"reak.5/7/16 Dr.T.V.Rao MD


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Reference&

•Ba&ic Prac"ica! Micro(io!o4 +Socie"4 for Genera! Micro(io!o4 ISF, ,, -

•Goo!e re&orce& fro$ i$ae&



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•Prora$ Crea"e* (4 Dr.T.V.Rao MD fo(ene6" of Me*ica! Micro(io!oi&"& f

!earnin (a&ic %rinci%!e& in inoc!a"inc!"re %!a"e a& a Mi&&ion for Uni5e

e*ca"ion of &ki!!& in Micro(io!o4• E$ai!

• *oc"or"5raoH$ai!.co$

mailto:[email protected]:[email protected]

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Streaking culture plates in bacteriology by Dr.T.V.Rao MD