OLR (1982) 29 (12) E. Biological Oceanography 795

(46%) and Hansenula anomala (43%). Centre of Post-grad. Instruct. & Res., Univ. of Bombay, Panjim, Goa 403 001, India.

82:6227 Paul, J.H. and Brenda Myers, 1982. Fluorometric

determination of DNA in aquatic microorganisms by use of Hoechst 33258. Appl. environ. Micro- biol., 43(6): 1393-1399.

A simple, rapid method can detect from 0.05 to 10/~g DNA in bacterial cultures or natural water samples. The method is specific for DNA; DNase I treatment of extracts of natural microbial populations removed 95-100% of the observed fluorescence. DNA content ranged from 165 ng/mL for relatively eutrophic Potomac River water to 27 ng/mL for coastal Atlantic Ocean water and was correlated to an acridine orange direct count (r -- 0.90). Naval Res. Lab., Washington, D.C. 20375, USA.

82:6228 Rinne, Ilkka, Terttu Melvasalo, Ake Niemi and

Lauri Niemisto, 1981. Studies of nitrogen fixa- tion in the Gulf of Bothnia. Finn. mar. Res., 248:117-127.

Measurements in 1978 and 1979 (by the acetylene reduction method) showed that nitrogen fixation was negligible in the Bothnian Bay and that the level of nitrogen fixation was markedly lower (1/10) in the central and southern Bothnian Sea than in the northern Baltic Proper and the Gulf of Finland. Helsinki City Water Lab., Kylasaarenkatu 10, SF-00550 Helsinki 55, Finland.

82:6229 Simidu, Usio, Kumiko Tsukamoto and Yoshiharu

Akagi, 1982. Heterotrophie bacterial population in Bengal Bay and the South China Sea. Bull. japan. Soc. scient. Fish., 48(3):425-431.

Vibrionaceae, particularly abundant from 10 to 400 m, predominated at all the sampling stations; they averaged 32% among the isolates. Pseudomonas spp. were abundant at the South China Sea surface, comprising 44% of the isolates. Gram-positive bacteria occurred mostly in water deeper than 800 m. Ocean Res. Inst., Univ. of Tokyo, 15-1, Minami- dai-l, Nakano, Tokyo 164, Japan.

$2:6230 Tabor, P.S. and R.A. Neihof, 1982. Improved method

for determination of respiring individual micro- organisms in natural waters. Appl. environ. Micro- biol., 43(6): 1249-1255.

Zimmermann et al. (1978) combined the determi- nation of direct bacterial counts by fluorescence

microscopy with the determination of individual organisms with ETS activity by reduction of INT to INT-formazan. Improvements by Tabor and Neihof have enhanced the visual clarity of prepared sam- ples, resulting in greater counts of both total and INT-reducing bacteria. INT-reducing microorgan- isms were 61% of total counts in Chesapeake Bay, 2- to 10-fold greater than percentages previously reported for marine or freshwaters. Environ. Sci. Div., Naval Res. Lab., Washington, D.C. 20375, USA. (mjj)

82:6231 Vrijmoed, L.L.P., I.J. Hodgkiss and L.B. Thrower,

1982. Seasonal patterns of primary colonization by lignicolous marine fungi [38 spp., 3 unidenti- fiedl off Hong Kong. Hydrobiologia, 89(3):253- 262. Dept. of Bot., Univ. of Hong Kong.

82:6232 Yayanos, A.A. and A.S. Dietz, 1982. Thermal

inactivation of a deep-sea barophilie bacterium, isolate CNPT-3. Appl. environ. Microbiol., 43(6): 1481-1489.

Isolate CNPT-3 lost colony-forming ability and underwent morphological changes when exposed to temperatures of 10--32°C at atmospheric pressure. Indigenous deep-sea bacteria may be both barophilic and psychrophilic, and may have been missed in past studies due to sample warming. Scripps Inst. of Oceanogr., La Jolla, Calif. 92093, USA. (mjj)

El80. Biochemistry

82:6233 Sueur, S., C.M.G. van den Berg and J.P. Riley, 1982.

Measurement of the metal complexlng ability of exudates of marine macroalgae [with the MnOz exchange method]. LimnoL Oceanogr., 27(3): 536-543. ENSCL, B.P. 40, 59650 Villeneuve d'Ascq, France.

82:6234 Truchot, J.-P., A. Duhamel-Jouve, L. Barthelemy, A.

Belaud and C. Chastel, 1980/82. Effects of oxygen and carbon dioxide on some sea organ- isms. Seminar 2 December 1980. Oceanis, 8(1): 1-27; 3 papers. (In French, English abstracts.)