Study on Vegetative Propagation using bulbs of Wild …escipub.com/Articles/JHMR/Vol1/Patel-JHMR-07-2016.pdftheir vegetative parts and/or by the modified structures like bulb, tuber,

*Correspondence to Author: D. K. PatelDepartment of Rural Technology, Guru Ghasidas Vishwavidyalaya, (A Central University), Bilaspur, 495009, Chhattisgarh, India. Emai l :dp lantsc [email protected]

How to cite this article:D. K. Patel. Study on Vegetative Propagation using bulbs of Wild onion [Urginea indica (Roxb.) Kunth] in Herbal Garden for Ex-situ conservation. Journal of Herbal Medicine Research, 2016,1(1): 0016-0025.

Received June 22 2016; accepted 7 July 2016; published 8 July 2016.

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Patel, JHMR, 2016; Vol. 1(1): 0016-0025

Journal of Herbal Medicine Research

(ISSN: 2474-106X)

Research Article JHMR (2016), Vol. 1, Issue 01

Study on Vegetative Propagation using bulbs of Wild onion [Urginea indica (Roxb.) Kunth] in Herbal Garden for Ex-situ conservation

Plants are showing variable tendency in their morphological structure and also in their habit, habitat and presence in nature. These are also performing a major role in construction of biodiversity in certain ecological areas. For seasonal appearances in nature plants are showing diversity in their propagation rate and modes. Seeds are important structure developed after fertilization in plants but the plants which does not includes seeds in their life are well potent to propagate using their vegetative parts and/or by the modified structures like bulb, tuber, rhizome etc.Wild onion [Urginea indica (Roxb.) Kunth] includes bulbs inside of soil which after maturation produces many small bulbs. These adventitious buds are separated from mother bulb carefully without damage the bulbs and are used as a plant source to develop new individuals of this plant.The current experiment was done in prepared poly bags (filled with soil, sand and manure mixture equally) followed by the proper supply of the water. During of the experimentation necessary facilities provided as per need of the developing bulbs to convert in to new plant. Above rapid propagation was made in herbal garden aimed for its propagation as well as for ex-situ conservation.Keywords:Urginea indica (Roxb.) Kunth, Bulb, Vegetative propagation, Herbal garden, Ex-situ conservation.

D. K. Patel *

Department of Rural Technology, Guru Ghasidas Vishwavidyalaya, (A Central University), Bilaspur, 495009, Chhattisgarh, India.

ABSTRACT

JHMR: http://escipub.com/journal-of-herbal-medicine-research/ 0016

INTRODUCTION

Wild onion [Urginea indica (Roxb.) Kunth] is a mem-ber of family Liliaceae and is a glabrous, bulbous herb. Commonly it is known as Indian squill and local-ly known as jangali pyaz. Assessment of plant diver-sity is remarkable for understanding the biodiversity in certain ecological areas. It is of a great importance for composition of biodiversity. Plants are showing variation in their life as well as propagation behaviour in nature. Morphological diversity of the plants are leading by the changes of environmental factors. The optimum level of the presence of such components supports the successful growth and development of the plants. Alteration of the environmental variables and their degree directly affect the growth of plant life.

Interaction between environment and biota is continu-ous process in nature which determines the presence of certain species in natural occurrence. To appear in nature, the plants are adapted in different ways. For this purpose these are producing rich amount of seeds with different adaption for their dispersal and germination capacity to develop new plants.

All the plants in nature are not capable to produce such type of seeds are of well potential to regener-ate using their vegetative plant parts like root, stem, leaf etc. Modifications of the vegetative structures are of a great significance for variety of plants such as for storage, for reproduction and for protection etc.

The structures of the plants like bulb, tuber, rhi-zome, corm etc. are also registered not only for storing foods materials but also participating role in vegetative propagation of the variety of the plant species which are not capable to produce seeds in their life phage. Its function is fulfilled by the mod-ified vegetative structures. These structures in the presence of favourable environmental condition germinate new buds which further convert in to new plants like their parental plants. It is a unique adaption of plants in nature to sustain and maintain their species diversity in different ecological sites.

The plant wild onion includes small bulbs, under-ground and is developing new small buds in presence of favourable climatic condition which further devel-ops new plant similar to their parental plants followed by the vegetative mode of plant propagation. Above practices done in herbal garden not only for their rapid multiplication but also for ex-situ conservation.

Cytotaxonomical studies in Indian Urginea stein-

hill species done by Yadav and Dixit 1990 [16]. Ab-bas et al 2012 [1] found gastrointestinal stimulant effect of Urginea indica Kunth.and involvement of muscarinic receptors. Bashir et al 2013 [2] Studied on bronchodilator & cardiac stimulant activities of Urginea indica. Biosystematics studies on medici-nal plant Urginea indica Kunth. Liliaceae: A review was made by Kameshwari et al 2012 [6]. Krenn et al 2000 [7] recorded new Bufadienolides from Urginea maritima Sensu strictu. Raphides in urginea indica kunth (Liliaceae) was found by Rao et al 2012 [11].

Urginea indica and its role in psoriasis: A Review made by Shiva et al 2012 [14]. Jagtapa et al 2014 [4] studied phytochemical Screening, Antioxidant Ac-tivity and Flavonoids Analysis of Bulb Extracts of Urginea indica Kunth. Kameshwari 2013 [5] ana-lyzed chemical constituents of wild onion Urginea indica Kunth Liliaceae. Murthy et al 2011 [8] focused on phytochemical analysis, in vitro anti-bacterial and antioxidant activities of wild onion sps. Chemical analysis of Urginea indica (roxb.) Kunth. Cytotypes was done by Patil and Torne 1981 [9]. Patil and Torne 1981 [10] fund vitamin contents in U. indica cytotypes.

Phytochemical analysis & In-vitro antimicrobial ac-tivity of Urginea indica was made by Rathabai et al 2012 [12]. Deepak and Salimath 2006 [3] noticed on antiangiogenic and proapoptotic activity of a novel glycoprotein from Urginea indica is mediat-ed by NF-kappa B and Caspase activated DNase in ascites tumor model. Chemical constituents of wild onion Urginea indica Kunth Liliaceae was done by Shiva and Kameshwari 2013 [13]. Phyloge-netic analysis among Indian squill Urginea indica Kunth. Liliaceae was done by Shiva et al 2013 [15].

MATERIAL AND METHODS

New bulbs of wild onion developed near the main bulbs are carefully removed individual-ly and planted in prepared poly bags for fur-ther development of new plants. During the de-velopment of this plant basic requirement were fulfilled by supplying water and other requirements.

Ten poly bags were filled with a mixture made by sand soil and manure in equal amount each. These poly bags are further applied to propagate the wild onion by vegetative mode using small bulbs. Above practice done in Herbal garden de-veloped for propagation as well as for ex-situ con-servation of varied Medicinal and Aromatic plants.

Patel, JHMR, 2016; Vol. 1(1): 0016-0025


RESULT AND DISCUSSIONS

It is a small bulbous herbaceous medicinal plant ca-pable for rich potential to regenerate itself by using its adventitious new bulbs developed near the main bulb of the plant underground. Its height is less than culti-vated varieties of onion. Bulbs are small, fleshy, un-derground providing adventitious root to support the plants. Roots are short, unbranched, originate from sin-gle center point of the bulb, Adventitious root system.

New small bulbs developing near of main bulb af-ter their maturation capable to convert in to new plant as their mother plant in presence of suitable environmental condition. Leaves are flate, long, thin, glabrous, shiny, green, parallel leaf venation, Aroma like cultivated species of onion present.

Flowers are in clusters, white in colour produc-ing annually at the ending of the plant life. Mod-erate level of water needed for its successful growth and development. Storing water near the plant is harmful so that should be removed.

Well mature plants bulbs were selected and are used to regenerate in different prepared poly bags [Fig. 1, 2]. After one month grown bulbs were well developed [Fig. 3, 4, 5] and are transferred to the prepared field [Fig 6, 7].

During the tenure of plant development all the nec-essary facilities were provided to the developing plants as per their needs like, water, temperature, light etc. Proper monitoring was followed to exam-ine the requirement of the plantlets in each one of the poly bags. The process was helpful for their rapid regeneration to support their conservation. The plant is well developing in humid soil with proper main-tained condition like Temperature, Moisture etc level.

Finally it is concluded that the steps should be fol-lowed to propagate this plant is small as well as for large scale. Developing new plants in poly bags are supporting their mass transfer in re-quired sites for growing/cultivation purpose.

ACKNOWLEDGEMENT

A research project start up grant sanctioned to me from UGC-New Delhi -India. I am thankful to them for their financial assistance helpful for current research.

REFERENCES

1. Abbas, S., Bashir, S., Khan, A., Mehmood, M. H. and Gilani A. H. (2012). Gastrointestinal stimulant effect of Urginea indica Kunth.and involvement of

muscarinic receptors. Phytother Res., 26: 704-08.

2. Bashir, S., Abbas, S., Khan A. and Gilani, A. H. (2013). Studies on bronchodilator & cardi-ac stimulant activities of Urginea indica. Ban-gladesh Journal of Pharmacology, 8: 249-254.

3. Deepak, A. V. and Salimath, B. P. (2006). Antiangiogenic and proapoptotic activity of a novel glycoprotein from Urginea indica is mediated by NF-kappa B and Caspase activated DNase in ascites tumor model. Biochmie 88:297-307.

4. Jagtapa, S., Satputeb, R. and Mulani, R. M. (2014). Phytochemical Screening, Antioxidant Activity and Flavonoids Analysis of Bulb Extracts of Urginea indica Kunth, International Interdisciplinary Research Journal, 4 : 170-186.

5. Kameshwari, M. N. (2013). Chemical con-stituents of wild onion Urginea indica Kunth Liliaceae. International Journal of Phar-macy & Life Sciences, 4(2) : 2414 - 2420.

6. Kameshwari, M. N. S, Lakshman, A. B. and Paramasivam, G. (2012). Biosystematics studies on medicinal plant Urginea indica Kunth. Liliaceae: A review. IJPLS. 3 : 1394-1406.

7. Krenn, L., Jelovina, M. and Kopp, B. (2000). New Bufadienolides from Urginea maritima Sensu strictu. Fitoterapia 71 : 126 - 129.

8. Murthy, P., Mamtharani, G., Tejas, D. R. and Suarlikerimath, N. M. (2011). Phytochemical analysis, in vitro anti-bacterial and antioxidant activities of wild onion sps., International Journal of Pharma and Bio Sciences, 2 (3) : 230 - 237.

9. Patil, M. M. and Torne, S. G. (1981). Chemical analysis of Urginea indica (roxb.) Kunth. Cytotypes. Curr. Sci., 50: 956-958.

10. Patil, M. M. and Torne, S. G. (1981). Vitamin contents in U. indica cytotypes. Indian drugs, 18: 345-350.

11. Rao, P. P., Shiva M. N., Kameshwari, Nijagunaiah and Saraswathi, K. J. T. (2012). Raphides in urginea indica kunth (liliaceae), Indian Journal of Plant Sciences, 1 (2-3) : 153-159.

12. Rathabai, V., Baskaran, C. and Shivmani. (2012). Phytochemical analysis & In-vitro antimicrobi-al activity of Urginea indica. Journal of Pharma-cognosy and Herbal Formulations, 2(10) : 6-12.

13. Shiva, M. N. and Kameshwari. (2013). Chemical constituents of wild onion Urginea indica Kunth Liliaceae Int. J. of Pharm.

Patel, JHMR, 2016; Vol. 1(1): 0016-0025


& Life Sci. (IJPLS) 4 ( 2) : 2414 - 2420

14. Shiva, M. N. Kameshwari and Paramasivam, G. (2012). Urginea indica and its role in psoriasis: A Review Int. J. of Pharm. and Life Sci. (IJPLS), 3 (12) : 2236 - 2242.

15. Shiva, M. N., Kameshwari, H. L. Geetha and Tharasaraswathi, K. J. (2013). Phylogenetic analysis among Indian squill Urginea indica Kunth. Liliaceae, Journal of Applied and Natural Science 5 (1): 10 -16.

16. Yadav, S. R. and Dixit, G. B. (1990). Cytotaxonomical studies in Indian Urginea steinhill species. Cytotlogia, 55: 293-300.

Patel, JHMR, 2016; Vol. 1(1): 0016-0025


How to cite this article:

D. K. Patel. Study on Vegetative Propagation using bulbs of Wild onion [Urginea indica (Roxb.) Kunth] in Herbal Garden for Ex-situ conservation. Journal of Herbal Medicine Research, 2016,1(1): 0016-0025.

Prepared poly bags

Plant selection

Figure 1 on 18 July 2015

Patel, JHMR, 2016; Vol. 1(1): 0016-0025


Grown bulbs in poly bags

Grown bulbs in poly bags

Fig. 2 28 July 2015

Patel, JHMR, 2016; Vol. 1(1): 0016-0025


Developing new bud/plant in poly bags

Developing new bud/plant in poly bags

Fig. 3 8 August 2015

Patel, JHMR, 2016; Vol. 1(1): 0016-0025


Plant growth in poly bags

Plant growth in poly bags

Fig. 4 A 18 August 2015/20 August 2015

Patel, JHMR, 2016; Vol. 1(1): 0016-0025


Mature plants in poly bags

Mature plants in poly bags

Fig.5 B 18 August 2015/20 August 2015

Patel, JHMR, 2016; Vol. 1(1): 0016-0025


Transfer of mature plants in Field

Transfer of mature plants in Field


Patel, JHMR, 2016; Vol. 1(1): 0016-0025


Plantation

Plantation


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Study on Vegetative Propagation using bulbs of Wild …escipub.com/Articles/JHMR/Vol1/Patel-JHMR-07-2016.pdftheir vegetative parts and/or by the modified structures like bulb, tuber,