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Simplify western blot imaging with iBright Imaging Systems
Invitrogen™ iBright™ Imaging Systems offer:
• Push-button, optimized exposure
• User-focused, intuitive interface and workflows
• Brilliant 4-color fluorescence multiplexing
Become a western imaging expert, instantly. See how the iBright Imaging
Systems can empower you to meet your western imaging challenges.
Download the brochure and request a demo
at thermofisher.com/ibright-science
For Research Use Only. Not for use in diagnostic procedures. © 2017 Thermo Fisher Scientific Inc. All rights reserved.
All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. COL04798 0917
Stunningly easy
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Western blotting
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Kong-YanWKong-YanWu et al. (Zhen-Ge Luo), “Semaphorin 3A activates the guanosine triphosphataseRab5 to promote growth cone collapse and organize callosal axon projections”, Sci. Signal. 7, ra81 (2014).
Rat Brain Slice. Image: Kong-YanWu and Zhen-Ge Luo, Chinese Academy of Sciences.
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LIFE SCIENCE TECHNOLOGIES
242 13 OCTOBER 2017 • VOL 358 ISSUE 6360 sciencemag.org/custom-publishing SCIENCE
Produced by the Science/AAAS Custom Publishing Office
new products: protein analysis
Magnetic Conjugation KitThe Magnetic Conjugation Kit from Innova Biosciences allows researchers to covalently conjugate antibodies and proteins to 0.5-
µm magnetic particles without the need for extensive optimization of the conjugation reaction, thereby saving precious antibody or protein. Ideal for use in immunoprecipitation experiments, the kit features covalent attachment, which provides a more stable alternative to noncovalent antibody attachments using traditional protein A or protein G resin. The ���������������������������������������������� ������������������between high surface area and fast separation, providing a binding capacity twice that of leading competitors, and are able to achieve high ������������������������������������������������������������������binding.Innova Biosciences
For info: +44-(0)-1223-661000www.innovabiosciences.com
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Imaging System������"�!�������� �����������!�"!������!�!"����!���������!�!�is a heightened need for dependable multiplex Western blotting reagents and platforms. Multiplex Western blotting can be challenging, since it needs quite a bit of protocol optimization to detect proteins of varying abundance ����!���!��������������������������������������!�������������"������!�"����������� !"�����"�������������������!�"!��������������"��������make this process easy, Bio-Rad has launched a solution that helps scientists do more with less sample and reagents. The ChemiDoc MP Imaging System (with red-green-blue (RGB) and near-infrared capabilities), combined with ��!�� !"��"���������!��!��!������� ������!����!"������� �������������!��(the ultrasensitive StarBright Blue 700 Fluorescent Secondary Antibodies and the hFAB Rhodamine Anti-Housekeeping Antibodies) and stain-free gels, makes up a unique, powerful toolbox, allowing the detection and ������"��������� �������!!� ���!�����������!������������������ ����reprobing, or cutting the membrane.Bio-Rad
For info: 800-424-6723www.bio-rad.com/en-us/product/chemidoc-mp-imaging-system
UHPLC Columns and Solvents
��,���,�����*�,���-�,���-������*�������������+,�����-,��������chromatography (UHPLC) columns and Fisher Chemical Optima UHPLC-MS *���,�*���,�*+,-��-������,*��,�����-�+�,,����,������+,�����-,������*�� !�"#�*�*�,*����,�-����+�������������-��-�*�*�*����$%� !�"#�-���*����,�*������,����,����*,�,-�����������������+���*��������,�,��applications, such as rapid screening for drugs of abuse, pesticide studies, and metabolite and peptide analysis. The Fisher Chemical Optima UHPLC-MS solvents are high-quality, ultrapure, and designed to address the needs of chromatographers performing state-of-the-art UHPLC. These solvents are ideal for detecting trace amounts of analytes by tandem mass spectrometry &'�('�)��*���,���,�������,��������,-����������*�*��������� �*,��,�
interference. They provide a very low mass/noise level in both positive- and negative-mode ionization, minimal metal ion content, and a low !�"#� ���,*+�*,��*���+��������,��������,�,-������ ������� ����� �����
For info: 800-955-6288������(���&�(��)�
Bis-Tris Protein Gels
RunBlue Bis-Tris Protein Gels by Expedeon provide an alternative to NuPAGE Bis-Tris gels. These protein gels run with the same voltages, in ��(�&�(����&������&(���(�&�(������ ��� ��(�&��&�!���"���(�&��RunBlue Bis-Tris gels achieve highly comparable results with exceptional reproducibility and resolution. Expedeonís new Bis-Tris gels can be ��������&��������������� ��� ��(�&��('(��������(���()�����weight range of proteins to be separated, and use the same LDS sample ��(�����'����)��&��&�!���"���#$$�������(�)�&&(��(&��������(�!���"��SureLock tank without any need for adaptors or spacers, and gels are ��&��)�'��� �(��������(��%�������� �������&�&�(����(�(��&�����������disruption, no downtime, and no transition period.ExpedeonFor info: 844-611-3656www.expedeon.com
Single-Cell Western BlotsMeet Milo. He does Single-Cell (sc) Westerns thatíll let you measure protein expression in thousands of single cells in a single run. Youíll get ���������(���#�������&����������(���������&�(��&�('���������)���&(�� ���(�&�(���'��������(&�(���������(&����$������������������cytometry. Milo makes measuring protein expression heterogeneity and identifying cellular subpopulations a breeze. Single-Cell Westerns happen on scWest chips. Put your cell suspension on the chip and put it in Milo. He captures 1,000+ cells, lyses them, runs an SDS-PAGE separation on every single cell, and immobilizes all your separated '���(�&���%�����(&������!(���&��'���'���(������'���(������(�&�on-chip with standard primary and secondary antibodies. One simple process lets you measure diverse targets and detect surface and intracellular proteins simultaneously. You can image probed scWest )��'&����������'(�'������������(&)()(���)��������&)�(���ProteinSimpleFor info: 888-607-9692www.proteinsimple.com
Competent Cells���� '�(&&��&�����(�����&�����������)�(�����&������(&��(������high-level protein expression. This next-generation protein expression system can replace slow-growing Escherichia coli systems prone to low ��(��&������(��((����������&�����(��)��&�������(&������)(��&���(�derived from the marine micro-organism Vibrio natriegens. This gram-negative, nonpathogenic bacterium doubles twice as fast as E. coli, produces greater amounts of biomass, and generates larger amounts ���'���(������&����(�����(�'(�������������'���(��('�(&&�����������similar to E. coli, ���� '�(&&��&�)��'�����(������'��&���&����antibiotics commonly used with bacterial expression systems such as E. coli BL21(DE3). Built with a tightly controlled, IPTG-inducible T7 '�����(��&�&�(������� '�(&&�)(��&�)���(�)�����(���&��������(�growth medium as well as commercial autoinduction media or our ���� ��)�(��"�������(������()���(�(��������'�������������greatest accumulation of biomass). SGI-DNA
For info: 855-474-4362https://sgidna.com/vmax-express.html
Global bio-techne.com [email protected] TEL +1 612 379 2956 North America TEL 800 343 7475
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For research use or manufacturing purposes only. Trademarks and registered trademarks are the property of their respective owners.
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