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Supercritical Fluid ChromatographyLow-Cost, Fast, Green Technology
The JasCo advantageJASCO’s modular SFC/SFE platforms have been optimized and refined over the last 20 years to provide reliable, worry-free performance for a wide variety of applications.
• ThepatentedJASCOback-pressure regulatoremploysahigh-speedswitchingvalve toensurethataconstantbackpressureismaintainedatalltimes,regardlessofthegasflowrate.Mostothersystemsusearestrictiondevicewhich isflowdependentandhence isunabletoprovidetheessentialconstantpressureconditions.TheJASCOdesignhastheadditional benefit of very low dead volume (below 10 µL) which prevents fractions from re-mixing.Italsopresentsasignificantreductioninanyprecipitationbuildupintheflowline.
• JASCOoffersawiderangeofdetectorswithhighpressurecells–UV,DiodeArray(real-timecollectionof3-Dspectraandchromatograms),andtheonlyCDdetectoravailableforSFC.
• OuranalyticalSFC/SFEsystemscanbeconvertedeasilytoHPLC.
• JASCO’smodifierdeliverypumpsguaranteestabledeliveryperformanceevenathighflowrates.
• JASCOoffersatrulymodularsystem–fromasimpleextractortoacomplexmulti-instrumentsystem that shares the same basic components.
• JASCOSFCsystemsarecompatiblewithmostMassSpectrometersandofferdirectcontrolin both Excalibur and Analyst software.
• JASCOprovidesprofessional,fastandcourteoussupportonallofourproducts.Wehave33differentservicecentersintheU.S.toensurethehighestlevelofcustomersatisfaction.Ourscheduledandon-sitetrainingclassesareavailableforadvancedapplicationtraining.
• JASCOoffersmulitpleconfigurations,pleaseaskyourlocalsalesrep.
Low-Cost, Fast, Green Technology over the years, JasCo has responded to the growing emphasis on
reducing chemical waste by offering an alternative to traditional
HPLC with a full line of “green” sFC/sFE products. The reduction in the
use of organic solvents has cost, health, and safety benefits as well as
faster, cleaner sample recovery during experimental procedures. These
advantages are a result of supercritical fluids liquid-like densities offering
higher solubility and increased column loading. They have low viscosity
and are highly diffuse enabling faster separation and extraction.
2
Contents
2 Features and Benefits
3 SFC Components
3-4 Carbon Dioxide Pumps
5-6 Modifier Delivery Pumps
7 Autosampler
8-9 Column Ovens
9-10 Detectors
11 Back Pressure Regulators
12 Temperature Control Units
13 Valves
14 Vessels
15 Analytical Systems
16-18 Extraction Systems
17-18 Fully Automated Residual Pesticide Extraction Systems
19-20 Preparative SFC
21 Software
22 Service and Support
23 Training Seminars
3
Supercritical Fluid Components
supercritical Carbon Dioxide Pumps PU-2080-Co2/PU-2086-Co2/PU-2088-Co2
Supercriticalfluidsystemsrequireapumpfortransferringliquidcarbondioxide.JASCOoffersthePU-2080-CO2 foranalysisapplications, thePU-2086-CO2 forapplications ranging fromanalysistopreparativeisolation,andthePU-2088-CO2forhigh-volume,preparativeisolation.
PU-2080-CO2 Supercritical Carbon Dioxide Pump (Analytical Range)Thiscarbondioxidetransferpumpisforanalysisapplications.ItemploysSlowSuction-QuickDelivery(SSQD),recognizedfor lowpulsationas itspumpingmechanism,therebyenablingstable transfer. It enables transfer at a stablecompositional ratiowhenusing it alongwithamodifierpump,therebyachievingseparationandextractionwithhighreproducibility.ThePU-2080-CO2alsomakesitpossibletobuildanextremelycompactanalysissystemwithoutrequiringanyexternalcoolingequipment.
PU-2086-CO2 Supercritical Carbon Dioxide Pump (Semi-Preparative)Thisdual-headsupercriticalcarbondioxidepumpisforapplicationsrangingfromanalysistopreparativeisolation.Itemploysaspeciallyshapedcamtoachievestablepumpingperformanceup toahighflowrate.Asinglesystemcanbeused toanalyzebatchesandperformpurityinspections.ItalsoconservesspacebycombiningthePU-2086withacompactcoolinghead.
PU-2080-CO2 PU-2086-CO2
4
Flow rate (mL/min) 0.2 0.5 7 15 120PU-2080-CO2
PU-2086-CO2
PU-2088-CO2
Delivery capacity at normal flow rate range
Model name PU-2080-CO2 PU-2086-CO2 PU-2088-CO2
Typeofpump SSQDmethod(SlowSuction-QuickDelivery)
Flowratesetting 0.001~10 mL/min 0.001~20 mL/min 0.1~150.0 mL/min
Flowraterange 0.2~7 mL/min 0.5~15 mL/min 5.0~120.0 mL/min
Pressurerange 0~30MPa 0~30MPa(lessthan15mL/min)0~25MPa(morethan15mL/min)
0~30MPa
Max. pressure 0~35MPa 0~35MPa 0~50MPa
Timeprogramming Flowrate,Upperpressurelimit,Lowerpressurelimit,solventswitvhing
Materials All materilas in contact with solvent are sapphire, ceramic, stainless steel, fluorocarbonpolymer,PEEK
Coolingmethod Peltierbuilt-incoolingjacket(circulationbathisrequired.)
Dimension 225(W)x430(D)x315(H)mmApprox.22kg
150(W)x575(D)x150(H)mmApprox.11kg
350(W)x700(D)x260(H)mmApprox.45kg
Powerinputvoltage AC100~240V,50/60Hz AC100~240V,50/60Hz AC100~120V,50/60Hz
Powerconsumption
500VA 185VA 600VA
PU-2088-CO2 Supercritical carbon dioxide pump (Preparative)This dual-head supercritical carbon dioxide pump is specifically designed for preparativeisolation.Dueto itsability to transfercarbondioxideatastable rateof10to120 mL/min,itcanprocesslargevolumesofsamplesuptoabout30mmofcolumninternaldiameter forpreparative SFC applications and up to 1L of vessel volume for extraction applications.
Supercritical Fluid Components
PU-2088-CO2
5
Modifier Delivery PumpsPU-2085/PU-2080/PU-2089/PU-2086/PU-2088Supercriticalfluidsystemsthatusecarbondioxideaddamodifiersolventwhenboostingthedegreeofextractionfreedom.Achievingastabilizedcompositionalratio(0.1to50%)iscrucialtoperformingseparationanalysis, requiringapumpthatperformsstablefluidtransfer.ThePU-2080,PU-2085,PU-2086,PU-2088,andPU-2089transferpumpsrenownedfortheuseinHPLC,aretheoptimalchoiceforpumpingmodifiersolvent.
PU-2085/2080 Semi-micro/Analytical HPLC pumpThese pumps are capable of low-pulse pumping due to optimizations of the pump head,checkvalve,plunger,andotherparts,therebydeliveringastablebaseline.JASCO’sproprietarySSQDpumpsdeliverco-solventatastableratewithlowpulsation.ThePU-2085isparticularlyefficientatpumpinginthelowflowrange.ThePU-2085andPU-2080areoptimalforpumping0.1 to 3 mL/min and 0.3 to 7 mL/min, respectively.
PU-2089 4-solvent low pressure gradient pump
ThePU-2089isanall-in-onequaternarypumpthatdeliversallthefunctionalityofthePU-2080HPLCpump, theLG-2080-04four-solvent low-pressuregradientunit,andtheDG-2080-54four-solventdegasser.ThePU-2089canswitchbetweenfourdifferentmodifiersolvents.
PU-2085/2080 PU-2089
6
Model name PU-2085 PU-2080 PU-2089 PU-2086 PU-2088
Typeofpump SSQDmethod(SlowSuction-QuickDelivery)
Flowratesetting 0.001~4 mL/min 0.001~10 mL/min 0.001~10 mL/min 0.001~20 mL/min 0.1~70 mL/min
Flowraterange 0.1~3 mL/min 0.3~7 mL/min 0.3~7 mL/min 1~15 mL/min 5~60mL/min
Pressurerange 0~30MPa 0~30MPa 0~30MPa 0~30MPa(lessthan15 mL/min)0~25MPa(morethan 15 mL/min)
0~30MPa
Max. pressure 0~50MPa 0~50MPa 0~50MPa 0~50MPa 0~50MPa
Timeprogramming Flowrate,Upperpressurelimit,Lowerpressurelimit,solventswitching
Materials Allmaterialsincontactwithsolventaresapphire,ceramic,stainlesssteel,fluorocarbonpolymer,PEEK
Dimension 150(W)x470(D)x150(H)mm
150(W)x470(D)x150(H)mm
150(W)x470(D)x225(H)mm
150(W)x470(D)x150(H)mm
350(W)x470(D)x260(H)mm
Weight Approx.10kg Approx.10kg Approx.14.3kg Approx.10kg Approx.34kg
Powerinputvoltage AC100~240V,50/60Hz
AC100~240V,50/60Hz
AC100~240V,50/60Hz
AC100~240V,50/60Hz
AC100~120V,50/60Hz
Powerconsumption 185VA 185VA 185VA 185VA 600VA
Flow rate (mL/min) 0.1 0.5 7 15 60PU-2085PU-2080/2089PU-2086PU-2088
Delivery capacity at normal flow rate range
PU-2086 Preparative pumpThispumpemploysadualplungersystemoptimalforhigh-flowpumping.Ithandlesapplicationsupto20mL/minandcanbeusedforanalyzingpreparativesamplesinjectedathighvolumes.TheunitisthesamesizeasthePU-2080andsavesspaceevenwhencombinedwithahigh-flowpumpingsystem.
PU-2088 High-! owrate pumpThisunitisamodifiersolventpumpdevelopedforpreparativeSFC.Theusualflowraterangeisbetween5and60mL/minandwhenusedwiththePU2088-CO2enablestheprocessingofhigh-volumesamples.
Supercritical Fluid Components
PU-2086 PU-2088
7
autosampler for sFCas-2059-sFTheAS-2059-SFautosamplerisusedtomeasuremultiple-specimensamples.
AS-2059-SF Autosampler When using supercritical carbon dioxide as a mobile phase, it replaces the content of thesampleloopwithsupercriticalcarbondioxidewitheachinjection.Continuousintroductionsofsamplesrequirestheaspirationofthenextsampleaftersafelydischargingthecarbondioxideinthesampleloopandfullyreplacingitwithanorganicsolvent.TheAS-2059-SFcansafelydischargecarbondioxide in thesample loopandcontinuously injectsamples.The injectionmethodcanbeselectedaseitherafixedinjectionvolumethatfillstheentiresamplelooporavariable injectionvolume that introduces thesample inaportionof thesample loop.Thevariableinjectionvolumemethodisalsocapableoffullinjectionwithzerosampleloss.
Temperature ControlTheoptionalsample-warmingunit(TC-2059)cancontrolthetemperatureofasamplebetween4ºCand60ºCin1ºCincrements(temperaturecontrolprecision:±1ºC).
Sampleinjectionmethod: Variablesamplevolumeinjectionmethodorfixedvolumeinjectionmethod
Numberofsamples: 120(2.0µLvials,standardrack)54(Largevolume,4.0mLvials)224(300,600µLvials,micro-volumerack)384x2(384-wellsmicroplateX2,microplaterack)
Sampleloop: 5 µL
Injectionvolume: 0.1to100µL(0.1µLincrements)(Standard,volumesover10µLrequireloopreplacement.)1to1000µL(1.0µLincrements)(Optional,largevolumeinjectionkitisrequired.)
Reproducibility: RSD0.5%orless(Whenfixedloop,20µLinjection)
Contamination: 0.01%orless(Whenfixedloop,5µLinjection)
Injectionspervial: 1to99
Analysistimesetting: 0.1to999.9min(0.1minincrements)
Max.usablepressure: 30MPa
Temperaturesettingranges: Optional,4°Cto60°C(Precision:±1°C)
Materials: All materials in contact with solvent are ceramic, stainless steel, fluorocarbonpolymer,PEEK
AS-2059-SF
8
Supercritical Fluid Components
Column ovensCo-2060/Co-2065/sFC-sroDensitychangesduetochangesintemperaturehaveamajorimpactontheseparationandextractionprocessonsupercriticalfluidsystems.Achievingstableextractionefficiencyandpeakretentiontimesrequirestheuseofanoventopreciselycontrolextractionvesselsandregulatecolumntemperature.JASCOofferstheCO-2060,which iscapableof temperaturecontrol at or below room temperature and the CO-2065 and SFC-Sro. The SFC-Sro isdesigned for temperature control for cup-shaped, high-pressure vessels and it includes amixingfunction.
CO-2060/2065 Column OvenThe CO-2060/2065 ovens are capable oftemperature control with a precision of ±0.1ºC with excellent reproducibility. TheCO-2060 is for heating and cooling, whiletheCO-2065isforheatingonly.Bothmodelsdetect abnormal temperatures and solvent leaks and include a function for shuttingoff theheat.A manual indicator,preheatingcoil, reaction coil, and other such devices can be installed on both the CO-2060 andthe CO-2065. Furthermore, the CO-2060caneasilyperformnotonlycooling(usedforoptical isomer separation columns), but also temperature increases and decreases by the useofatimeprogram.
SFC-Sro Column OvenThe SFC-Sro oven is capable of heatingfrom room temperatures +20ºC to 150ºC and supports not only ordinary columns and extraction vessels, but also cup-shaped, high-pressure vessels. Inserting a mixerenables the efficient mixing of the vessels’contents,improvingreactionefficiencywithinsupercritical carbon dioxide.
CO-2060
SFC-Sro
CO-2065
9
DetectorsUV-2070/2075/MD-2010/2015/CD-2095Evaluatingopticalcharacteristicsunderasupercriticalstate requiresadetectorandahigh-pressureresistantcell.MonitoringsamplesinsupercriticalfluidispossiblebyreplacingtheflowcellsectionofJASCO’sliquidchromatographydetectorwithahigh-pressureresistantcell.
UV-2070/2075 UV/Vis DetectorTheUV-2070/2075UV/Visdetectorcombineshigh performance, multifunctionality, andsuperior operability for most standard detectiontechniquesinchromatography.
Model name CO-2060 CO-2065 SFC-Sro
Temperaturecontrolmethod Heater,Peltier Heater Heater
Temperaturecontrolrange 15ºCbelowambientto80ºC 10ºCaboveambientto80ºC 20ºC above ambient to 150ºC
Accuracy ±0.1ºC(Whentemperatureissetat40ºC.)
±0.1ºC(Whentemperatureissetat40ºC.)
±0.3ºC
Stirrer None None Included(100to1000rpm)
Safetycheck Heaterpowershutoffwhenunusuallyhightemperatureorflammablesolventleaksaredetected.
Heaterpowershutoffwhenunusuallyhightemperatureorflammablesolventleaksaredetected.
Heaterpowershutoff when unusually hightemperatureisdetected.
Column compartment 110(W)×415(H)×83(D)mm 110(W)×415(H)×83(D)mm 240(W)×245(H)×200(D)mm
Dimensions 150(W)×465(H)×470(D)mm 150(W)×465(H)×470(D)mm 310(W)×460(H)×460(D)mm
Weight Approx.19kg Approx.15kg Approx.20kg
Powerrequirements 100~220V,AC,50/60Hz 100~220V,AC,50/60Hz 100~120V,AC,50/60Hz
Powerconsumption Approx.355VA Approx.230VA Approx.650VA
UV-2070/2075
10
Supercritical Fluid Components
MD-2010/2015 Diode Array DetectorMultichanneldetectorsarecurrentlyusedinthesamemannerasgeneral-purposedetectors.TheMD-2010and2015deliverhighresolutionandhighsensitivityasaresultofanoptimizedopticalsystemandredesigneddataprocessingcircuits.Theyalsocanbeusedasstand-alonedetectors, even without a data station. These detectors are equipped with a four-channelanalogoutputterminal.Bothtimeandwavelengthaccumulationcanbesetandsamplescanbe measured in the optimal state for the desired sensitivity and resolution.
CD-2095 Circular Dichroism Chiral DetectorThis detector measures optical isomers with circular dichromatic absorption between 220and 420 nm with high sensitivity and excellent selectivity. The CD-2095 is generally 10 to100timesmoresensitivethananopticalrotationdetector.ItcanmeasurebothCDandUVchromatogramsaswellasg-factor(CD/UV)chromatograms.Sinceg-factorinparticularhasaproportionalrelationshipwiththecompositionalratioofopticalisomersamples,theCD-2095can perform compositional measurements and high purity fractionation for non-separatedpeaks. In addition, spectral measurements for CD and UV are possible by stopped-flowspectrometry.
SFC High Pressure Flow Cells
P/N Detector Description
6824-H105A UVDetector High pressure flow cell unit, L=5mm, 4uL gasket: PEEK,window:quartz
6824-H118A UVDetector Highpressurepreparativecellunit,L=1mm
6831-H113A Multi-Channel Highpressureflowcellunit,L=5mmgasket:Pb,window:Detector quartz
6831-H114A Multi-Channel Highpressurepreparativecellunit,L=1mmgasket:PEEK,Detector window:quartz
6838-H106A CDDetector Highpressureflowcellunit,L=20mm
6838-H107A CDDetector Highpressurepreparativecellunit,L=1mmgasket:PEEK,window:sapphire
MD-2010/2015 CD-2095
11
Back Pressure RegulatorsBP-2080/BP-2080-MChanges inpressurecangreatly vary thecharacteristicsofSFC.Achievingextractionandchromatogramswithhighreproducibilityonasupercriticalfluidsystemrequiresadevicethatcanperformpressurecontrolinastablemanner.JASCOofferstheBP-2080,whichiscapableof automatic pressure control, and the BP-2080-M, which is capable of manual pressurecontrol.
BP-2080/2080-M Back Pressure RegulatorThe BP-2080 and BP-2080-M employa patented flow-switching valve (FSV)mechanism that enables stable system pressure control even at the lowest volume limitpossible.TheFSVmechanismregulatespressurebycontrollingthechannelopeningwhile a needle vibrates at a high rate ofspeed. This prevents blockages due tohigh-viscosityelutionsamplesandsolidifiedsamples, which can be a problem with static pressure regulators. This enables stablesystem pressure, even during long-termcontinuous operation. Since the BP-2080hasatimeprogramfeaturethatcancontrolpressure and temperature according to thepassageoftime,itmakesiteasytoautomateanalysiswork.TheBP-2080Moffersmanualcontrol for the volume passing through theopeningintheFSVmechanism.
SFC Vch-Bp 6-Way Valve Changer- Fraction CollectorThe SFC-Vch-Bp is an option for the BP-2080 that can be attached to the pressurecontrolsectionof theBP-2080/BP-2080-M.It features a one-channel switching valveandonehigh-pressure1-in-6-outvalve.Thisoption automatically separates the fluideluted from the BP-2080 into test tubes orsample bins. The channel switching valveis used for selecting either collection ordischarge, while the high-pressure 1-in-6-out valve connects downstream from the channelswitchingvalvetofunctionas6-waybranchingvalve.
BP-2080 BP-2080-M
SFCVch-Bp
12
Supercritical Fluid Components
Temperature Control UnitsHC-2068-01/HE-2068-01-04/CH-201/CCa-1111 Achieving extraction and chromatograms with high reproducibility on a supercritical fluidsystemrequiresstabletemperaturecontrol.
HC-2068-01/HE-2068-01-04 Heater ControllerTheHC-2068-01isatemperaturecontrollerforheatingsupercriticalfluid.HE-2068-01to04areheatexchangersthatareusedinconjuctionwiththeHC-2068-01,andarenecessarywhenperformingpreparativechromatographyandhigh-volumeextraction.Theseheatexchangersareconstructedwithstainlesssteelpipingandareusedtocontinuouslyheatthefluidflowingwithinthepiping.TheHE-2068-01ismainlyusedforheatingfluidbeforepreparativeisolation.TheHC-2068-02isprimarilyusedafterpreparativeisolation,whiletheHC-2068-04isusedtopreventicingattheoutputoftheautomaticpressurecontrolvalvesandtoheatsamples.
TI-2068-01 Temperature IndicatorTheTI-2068-01isacompact,space-savingtemperatureindicator.Multipleunitscanbeusedsimultaneously.
Circulating Thermostatic BathsThese units use a variety of circulating thermostatic baths to cool carbon dioxide for the PU-2086-CO2andPU-2088-CO2 as well as to circulate the heat that controlls the temperature ofthehigh-pressurevessels.
HC-2068-01
T1-2068-01
HE-2068-01 HE-2068-04
13
HE-2068-04
SV-500 SV-500-8 Safety valve
ValvesHV-2080-01/HV-2080-03/sFC-Evc-sr/sV-500-8/safety ValveJASCOoffersavarietyofvalvesavailableforsystemupgrades,includingautomaticvalvesforchannelswitchinganddistributing,stopvalvesforcuttingoffchannels,andmechanicalsafetyvalvesforensuringsystemsafety.
HV-2080-01/hV-2080-03/SFC-Evc-Sr Valve UnitTheHV-2080-01isatwo-channelselectionvalve,whiletheHV-2080-03isa1-in-6distributingvalve.Bothmodelsarehigh-pressurevalvesforautomaticallycontrollingchannelswitching.TheSFC-Evc-Sr isahigh-pressurevalveunit that incorporates two1-in-6-out valves. It isparticularlyusefulincombinationwiththeSCF-Sroforswitchingbetweenmultipleextractionvessels.
SV-500/SV-500-8 Stop ValveThesevalvesareusedtomanuallyopenandclosethechannel.Whenusingcarbondioxide,thesevalvescanstopitssupplytothesystemandmakepumpconditioningeasybyattachingonetotheoutputsideofapump.Thevalvesimprovesystemoperabilityandmaintainability.
HV2080-01 HV-2080-03 SFC-Evc-Sr
14
Supercritical Fluid Vessels
High Pressure Vessels
SFC-VCT
EV-1 EV-2 EV-3
SFC-VC1
View CellsViewCellsarecapableofextractingsamplesunderasupercriticalstateviatheobservationwindowandmonitoringtheirreactionprocessesinrealtime.JASCOofferstheSFC-VCIwithtwowindowsinthevesselcylinderandtheSFC-VCT,whichoffersthreewindowsthatprovidea view in the vertical direction as well.
15
Supercritical Fluid analytical
SFCThephysical characteristicsexhibitedby supercritical fluid includeadiffusioncoefficientofdissolvedmoleculesthatisahundredtimesgreaterthanitisinliquidandaviscositythatisatleastonefiguresmaller.Asupercriticalfluidchromatographysystem,whichemployssucha medium as a mobile phase, can be expected to serve as a separation analysis method that can rapidly perform separation without any drop in separation efficiency, even at fast flowrates,duetoarapidmasstransferinsidethecolumnwhencomparedwithhigh-speedliquidchromatographythatusesliquidasthemobilephase.
In addition, when carbon dioxide is used as the medium, gasification will occur simply bykeeping theseparatedand fractionatedsampleataconstant temperature,making thisoneofthetechniquescapableofhighlyefficientrefiningwithfewpost-processinghassles,suchaseliminationsolventsafterpreparativeisolation.Thisoffersahostofadvantages,includingcostsavingsrelatedtotheexpenseofpurchasingsolventanddiscardingorganicsolvents,andhighthroughputanalysisandpreparativeisolationduetorapidseparationoverashortperiodoftime.Asagradientelutiontechnique,SFCisabletovarythethreeparametersofpressure,temperature,andmodifiersolventvolume.Itcanbeeffectivelyusedwhenseparatingoligomersandconstituentswithdifferingcharacteristics.(Thetemperaturegradientrequirestheuseofacolumn oven that continuously varies temperature over time.)
0.000
0.020
0.040
0.060
0.080
0.0 1.0 2.0 3.0 4.0 5.0
HPLC
SFC
Measurement value
Column : OD
Pla
te h
eigh
t (m
m)
Velocity (mm/sec)
analytical supercritical Fluid Chromatography
16
Supercritical Fluid supercritical Fluid Extraction (sFE)
Supercritical Fluid Extraction (SFE) SystemsSupercriticalfluidextraction(SFE)isatechniquethataddsasupercriticalfluidofanextractionmediumtoasamplecontainingaconstituenttargetedforextraction,usingthedifferenceinsolubilitycomparedwiththeextractionmediumtocarryouttheextractionprocedure.Usingsupercritical carbon dioxide as an extraction medium has many advantages and is usedin a variety of fields. Extraction by means of supercritical carbon dioxide can be expected to improve efficiency, including shorter extraction times and simplified procedures whencomparedwithextractiontechniquesthatemployorganicsolvents.Inaddition,itofferseasiersolvent elimination and concentration procedures. Since its critical temperature is a low 31ºC or so, it enables extraction at a near-room temperature state or in a carbon dioxide atmosphere devoidofoxygen.Thismakesitatechniquethatisidealformaterialsthatexhibittemperatureinstability or constituents that are susceptible to oxidation. Furthermore, it has received recent attention as an environmentally friendly extraction technique that does not use hazardousorganicsolvents,ashasbeenadvocatedbythegreenchemistrymovementinrecentyears.
Applications forSFE include theextractionofactiveconstituents, includingvariousflavorsandmedicinal constituents fromnaturalproducts,docosahexaenoicacid (DHA), advancedunsaturated fatty acids and fatty esters such as eicosapentaenoic acid (EPA), fat-solublevitamins, and pharmaceuticals. Other applications include the elimination of unwanted constituents,suchasdecaffeinationanddesolvationwithintablets.Italsocanbeappliedtothepreprocessingofanalysissamples,includingHPLCandGC.
17
High pressure extraction vessel
If necessary, add heating coils, detector, and fraction collector.
Supercritical Fluid Extraction System – ConfigurationThe figure below shows the basic configuration and flow path of a supercritical carbon dioxideextraction system. In this system, a supercritical carbon dioxide pump, a modifier solventtransfer pump, an extraction vessel, a thermostatic bath, and an automatic back-pressureregulation valve are connected to the downstream end. The extraction vessel can either be acolumn or cupped vessel, depending on the state of the extraction sample. There is a widevariety of volume types. Extraction parameters include pressure, temperature, and co-solvent/volume. These parameters can be changed to set the optimal conditions.
Fully automated Residual Pesticide Extraction systems
Residual Pesticide Extraction System 3 (10mL)
18
Supercritical Fluid supercritical Fluid Extraction (sFE)
Residual Pesticide Extraction System 1 (50 mL)Attention is being focused on extraction techniques using supercritical carbon dioxide fortrace pesticides left behind on food products and in soil. Residual pesticide extraction systems that use supercritical carbon dioxide are a topic of interest at present. JASCO has a lineup of residualpesticideextractionsystemsequippedwiththesecharacteristics.
Features
• Simpleoperation
• Quickextraction-about 30 to 60 minutes per sample, which is one-third to one-forth the processing time of conventional techniques
• Eliminatestracepesticides-the extract is collected in a trap column
• Minimalextractcleanup-also roughly separates impurities in trap column
• Blockagesandcontaminationareminimized-the trap column and piping is washed each time
• Lowrunningcosts-carbon dioxide is highly pure and inexpensive
• Thesystemissafeandenvironmentallyfriendlybecausecarbondioxideisnon-toxicandnonflammable.
Flexibility
• Capacityofextractionvessels:10mLor50mL.The10mLvesselisidealifoperabilityisthefocus.The50mLvesselisabetterselectionifthefocusisonsampleprocessingvolume or analysis sensitivity.
• The50mLvesselofferssimpleone-touchattachmentandremoval.
• Onetosixvesselscanbeprocessedatatime.JASCOoffersthesinglevesseltypeorthesix-vesseltypedependingonthefrequencyofsamplesbeingmeasured.
19
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Column: CHIRALPAK IA(10mmIDx250mmL)CO2: 30ml/minModifier: CH3OH 4ml/min
Pressure: 20MPaWavelength: 240nmSample: Warfarin 15wt%, 800ml
UV CD
G-factorO
OH
CHCH2 COCOH3
O
Recovery 90%Purity 92%
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Column: CHIRALPAK AD-H
CO2: 15ml/minModifire: CH3OH 1.5ml/min
Pessure: 20MPaWavelength: 310nm
UV CD
G-factor
Theabovephotoshowsapreparativesupercriticalfluidchromatographysystemthatscalesuptheanalysislevel.ThefiguretotheaboveleftisanexampleofusinganI.D.10mmdiameterx250mmopticalseparationcolumnonthissystem,injecting250mgofwarfarinsampleeachtime,usingacirculardichroic(CD)detectorfordetection,outputtingUVandCDatthesametimeasg-factor,andmonitoringthepositionofthegoodstateofopticalpuritywhileisolatingwithhighefficiency.Thefiguretotheaboverightshowsthechromatogramsobtainedthroughcontinuous measurement.
Supercritical Fluid Preparative supercritical Fluid Chromatography
20
Supercritical Fluid Preparative supercritical Fluid Chromatography
TheSF-Box-01 and SF-Box-02 areusedforperformingprocesscontrolforotherunits.TheseunitsarerequiredwhenusingSF-NAV.SF-Box-01iscapableofinputtinganalogsignalsandcancarryoutfractiontriggersatdetectorlevelsandslopesinpreparativeSFC.SF-Box-02isthesameasSF-Box-01withouttheanalogsignalinputmodule.ItisusedmainlyforcontrollingSFE systems.
FC-2088-08 Fraction Collector for Preparative SFCTheSF-2088-08consistsofawarmingheaterplacedimmediatelyaftertheautomaticback-pressurevalve,avalveforswitchingbetweencollectionanddisposal,afractioncontrolvalve,agas-liquidseparatorandacollectionvial.Thesettingofvalvesandtemperatureisallperformedbyspeciallycontrolledsoftwareforsupercriticalfluid(SF-NAV).WithpreparativeSFC,alldevicesareinstalledinasinglebox,andanexhaustfanmaintainsdecompression.Thissuppressestheoutflowofcarbondioxideandmodifiersolventinthelab,therebyenablingpreparativeisolationin a clean environment.
ThePD-2000isadistributorforpowerandsignalcontrolandisrequiredwhenusingSF-NAV.ThefrontpanelfeaturesalargeACswitchthatcanturnonoroffsystempower.Itpreventsmalfunctionsbyallowingtheusertoturnsystempoweron/offwithasingleswitchoperationwhichisespeciallyusefulonsystemscomprisedofalargenumberofunits.Italsoplaysacrucialroleinsafesystemoperationbecauseitcandetectsystempowerleakagesandovercurrent.
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Supercritical Fluid software
EZChrom Elite™ Chromatography Data System for Analytical and Semi-Preparative SystemsComprehensive Data analysis
EZChrom Elite is a comprehensive software package that can flexibly handle all types ofchromatography data requirements and demands. These capabilities range from single-channeltodiode-arrayandspectraldatacollectionmodes.IntegratedGPC/SECandsystemsuitability functions are also available. Chromatographers can use EZChrom Elite with the confidencethattheyareusingapowerfulsoftwarepackagethatwillmeetalloftheircurrentandfuturelaboratorydataprocessingneeds.
• GLP/GMPfeatures
• Sensiblephotodiodearraydatahandling
• FullyintegratedtocontrolJASCO’s SFC/SFE,X-LC®andLC-2000Plusseries HPLCsystems
SF-Nav for Preparative SFCSF-NAVisaspeciallydesignedsoftwarepackageforthecontrolandprocessmanagementofSFEsystemsandpreparativeSFCsystems.Inadditiontopumps,autosamplers,anddetectors,itcanalsocontrolautomaticback-pressurevalvesandfractionation.Itcanmonitorandsaveinformation such as temperature, pressure, and CO2flowwithinthesystemduringpreparativeisolation and the extraction process, making it useful when investigating fractionation andextractionconditions. Itcanalsosetfractionationandextractionconditions in linewiththeslopeandthresholdvaluesofsignalsobtainedfromdetectorsandotherdevices.Itenablesfullprocessautomationforpreparativeisolationandextraction.Theprogramcanlisttheoperationstateofunits,showiconsthatinvokeprocessesbysample,anddisplaysamonitorscreenforchromatograms. Itcanalsobatch-saveparameters includingchromatograms,temperature,pressure, and CO2flowandexporttheminASCIIformat.
The operational state of the equipment modules and any abnormalities can be seen on the display. In addition, the display shows the temperature and pressure necessary for process management on a supercritical fluid system, including analog signals from detectors and other devices. The remaining volume in the gas cylinders is displayed based on the volume of CO2 consumed, as calculated according to flow settings.
EZChrom Elite™isatrademarkofAgilentTechnologies,Inc.5301StevensCreekBlvd.,SantaClaraCA95051
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Service and Support Committed to service Excellence
JasCo offers world class service and support. once an instrument is purchased, JasCo’s commitment to you is just beginning. We offer a multitude of services and programs that will keep your equipment operating at peak performance.
Choose the plan that is right for you. We offer four plans to make sure we have one that fits both your budget and requirements.
Advantage SupportTheAdvantageSupportplanofferstheultimatecoverageandincludes:
• Allparts,laborandtravelcosts
• TwoPreventativeMaintenance(PM)callsperyear
• OneIQ/OQservice
• OnefreecustomertrainingclassatJasco,Inc.
• Unlimitedphonesupportfortechnicalandapplicationissues
• 20%discountonconsumableparts
Full SupportTheFullSupportplanoffersexcellentcoverageataveryreasonablecosttoensureyourinstrumentisalwaysinoptimimrunningcondition.Thisplanincludes:
• Allparts,laborandtravelcosts,excludingconsumables
• CustomizedoneortwoPreventativeMaintenance(PM)callsperyear
• DiscountedIQ/OQservice(performedatthesametimeasPM)
• OnefreecustomertrainingclassatJasco,Inc.
• Unlimitedphonesupportfortechnicalandapplicationissues
• 15%discountonconsumableparts
Preventative Maintenance Support• OnePreventativeMaintenancecallperyear
• Unlimitedphonesupportfortechnicalandapplicationissues
• 5%discountonconsumableparts
JASCOoffersover30servicecentersstrategicallylocatedaroundthebusiestcustomer-based areas in North America to better serve our customers.
JASCO guarantees parts availability and support for 10 yearsafter purchase date of any SFC system or components.
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Application Training SeminarsQuality Training from Jasco, Inc.
Increasingproductivity inthelabrequiresreliable instruments,qualitysupportandastrongworkingknowledgeoftheinstrumentation.JASCOtrainingseminarsarespecificallydesignedtoincreaseyourknowledgeofJASCOproductsandrelatedapplications,aswellasinstrumentmaintenanceandtroubleshootingprocedures.
Weofferbothon-sitetrainingandregularlyscheduledtrainingseminarsatourEaston,Marylandapplications lab. For more information regarding pricing, times and course schedules, orregardingon-sitetrainingatyourfacilitypleasecontactusat800-333-5272.
Our seminars include: Circular Dichroism J-Series, FT-IR Spectroscopy, Fluorescence, UV-Vis/NIR,Polarimetry,HPLC,X-LC®,andSupercriticalFluidChromatography.
Thehands-onaspectofJASCO’sprograms,combinedwithsmallclasssizes,ensurethatyoureceivetheoptimumlearningexperience.
JASCO INTERNATIONAL CO., LTD.4-21,Sennin-cho2-chome,Hachioji,Tokyo193-0835,JapanTel:+81-42-666-1322Fax:+81-42-665-6512www.jascoint.co.jpAustralia,China,HongKong,India,Indonesia,Iran,Korea,Malaysia,NewZealand,Pakistan,Philippines,Russia,Singapore,SouthAfrica,Taiwan,Thailand
JASCO INCORPORATED28600Mary’sCourt,Easton,MD21601,U.S.ATel:+1-800-333-5272Tel:+1-410-822-1220Fax:+1-410-822-7526www.jascoinc.comCanada,CostaRica,Mexico,PuertoRico,Argentina,Brazil,Chile,Colombia,Paraguay,Peru,Uruguay
JASCO EUROPE s.r.l.ViaConfalonieri25,23894Cremella(Lc),ItalyTel:+39-039-956439Fax:+39-039-958642www.jasco-europe.comJASCODeutschlandwww.jasco.de,JASCOUKwww.jasco.co.uk,JASCOFrancewww.jascofrance.fr, JASCO Benelux www.jasco.nl, JASCO Spain www.jasco-spain.com,JASCO Scandinavia www.jascoscandinavia.se, Austria, Finland, Greece, Hungary, Poland,Portugal, Romania, Switzerland, Algeria, Cyprus, Egypt, Israel, Jordan, Kuwait, Lebanon,Morocco,SaudiArabia,Syria,Tunisia,Turkey,U.A.E.
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