Supercritical fluid methods

Jiří Konečný

2005

Sample is solid phase

SPME (Solid phase micro extraction)Sorbent is trapping contaminans from gas.

Biologic materials

Earth

CFE - Supercritical fluid extraction

Oven

Sample

Restrictor

CO2

Ectract + CH3OH

Extractioncell

Supercriticalfluid

cilinder

Pump

CO2

CH3OH

SFC - Supercritical fluid chromatography

EctractInjector

Pump

CO2

Supercriticalfluid

cilinder

CO2

Waste

Chromatogram

Oven Detector

Collum

InterfaceRestrictor

Why use supercricitcal methods?

density

g/ml

Difuzion coeficient

dinamic viscosity

coeficient

presure

GC 0,2 10-5 10-1 0,5-2 MPa

CFC 600 10-8 10-4 to 65 MPa

LC 800 10-9 10-3 10-40 MPa

Propertis of medium are difrent.Sample is better elution.

Using

Analytical chemistry – search for new substance and analyzes concaminans in eviroments

Industry – izolation of parfume and special substaces in famakology

Information sources

• Lecture analytical chemistry by Prof. RNDr. Sommer, DrSc

• Prof. RNDr. Sommer, DrSc : Základy analytické chemie• www.ashlandanalytical.com/ tour-sea.htm

• http://www.pioneerherbs.com/super_critical.htm