46
Supplemental data file for Zhai et al. Predicted secondary structures for new Glycine max miRNAs. The following pages are secondary structures of miRNA precursors generated using the web based service from CentroidFold (http://www.ncrna.org/centroidfold) (Hamada et al. 2009). Since miREAP checks only for the basepairing of miRNA/miRNA* but not the overall secondary structure of the precursor, CentroidFold (Hamada et al. 2009) was used to predict each precursor structure and these were checked by eye to remove those that do not have a typical stemloop. For each sequence, we have shown the output of CentroidFold, which includes both a standard basepair notation and a graphical representation of the folded sequence. In the folded sequence, the nucleotides are colored with a color gradation from blue to red which corresponds to a probability of pairing, scored in the likelihood range of 0 to 1.

Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

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Page 1: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Supplemental data file for Zhai et al.  Predicted secondary structures for new Glycine max miRNAs.  

 

The following pages are secondary structures of miRNA precursors generated using the web based service from CentroidFold (http://www.ncrna.org/centroidfold) (Hamada et al. 2009).  Since miREAP checks only for the base‐pairing of miRNA/miRNA* but not the overall secondary structure of the precursor, CentroidFold (Hamada et al. 2009) was used to predict each precursor structure and these were checked by eye to remove those that do not have a typical stem‐loop.   

For each sequence, we have shown the output of CentroidFold, which includes both a standard base‐pair notation and a graphical representation of the folded sequence. In the folded sequence, the nucleotides are colored with a color gradation from blue to red which corresponds to a probability of pairing, scored in the likelihood range of 0 to 1. 

 

Page 2: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

CentroidFold predicts an RNA secondary structure from an RNA sequence. FASTA and one-sequence-in-a-line format are accepted for predicting a secondary structure per sequence. It also predicts a consensus secondary structure when a multiple alignment (CLUSTALW format) is given.

Seq/Fold: >soy-m0075 GGAAGAAGAGCTTGAGGAAGTGATGGGAGATGGGAGGGTCGGTAAAGGATAACAGCGTCTCTATGATTAATTGTTGTGTTGTTTATTCTTTTGCCGATTCCACCCATTCCTATGATTTTCTTTGGTTCCTTTCTTTC ((((((((((((.((((((((.((((((.(((((.(((((((((((((((((((((((..(.(.......).)..)))))))).).)))))))))))).)).))))))))))).)))))))).)))))..)))))))

Energy: -54.20 Kcal/mol

Graph:

Page 3: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m0077 GATTGGAAGCATGAGCTTCAAAACCAAGAGGTTGTTTAGAGATTGCCTTCACAACAAATTTTCTATTGCATTCTTGATATGTAGACCCTCCTCTGATGCATTTGTAATGATGATCCAAACTTGGTTGTTGGAACCTCAAAACATTATGTTCTATTTGAGTTTCCCTTTTGTGTCGTGAAGCTTTTGATATCCAAGA ..(((((..((.((((((((...(((((((...................................................................................................((((.((((((.............)))))).)))))))))).)...)))))))).))...)))))..

Energy: -27.05 Kcal/mol

Graph:

Page 4: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m0119 ATTGATTCATCATGGAAGTGAATCGGGTGACTCTTCCATGTTGATCTCTCCTCCACCACCTCCTTAAGGTGATGGCAAATTTCTGAGTTTCCCGAATTTGCTTCCATGGTGAATCAATC (((((((((((((((((((((((((((.(((((........((.........(((.(((((.....))))).))))).......))))).))))).)))))))))))))))))))))).

Energy: -51.96 Kcal/mol

Graph:

Page 5: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m0126 TGTTAGACCAGTCAGCTGAATCCTCTTATGATCAAGAGCTGTAATGCATATCAGTTTTGTTATCAGTTTGAACCGTATTTTCTTTGAATTACCGTAACAGCCCTTCATCATAGGAGGAATCAACTGGCTGGTCTAACA (((((((((((((((.(((.((((((((((((.(((.(((((.(((..............................................))).))))).))).)))))))))))).))).)))))))))))))))

Energy: -55.45 Kcal/mol

Graph:

Page 6: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m0284 GGTTATGGTCTAACCCGTCTTAGTATAGTTCACTTTCTACGACGGTTATACATGTAACCGTATTAGAAAGTGAACTATACAAAGACGGTTATTCCAAAAAC .....(((..(((.(((((((.(((((((((((((((((..((((((((....))))))))..))))))))))))))))).))))))))))..))).....

Energy: -51.70 Kcal/mol

Graph:

Page 7: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m0285 GGTTATGGTCTAACCCGTCTTAGTATAGTTCACTTTCTACGACGGTTATACATGTAACCGTATTAGAAAGTGAACTATACAAAGACGGTTATTCCAAAAAC .....(((..(((.(((((((.(((((((((((((((((..((((((((....))))))))..))))))))))))))))).))))))))))..))).....

Energy: -51.70 Kcal/mol

Graph:

Page 8: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m0327 TCACAGCACAACCAGTATTCTTGTCCGCGGAAGGACCACCCCCGACGGCCAAAGAAAGAAGGAAACTCGATCTCCTCGAGGAGAGATTGAGGGTTGTTTAAGGCTTTGGTGACTATCCATTCGCGGACATGATGGATCTTTGCTTGG ....((((...(((....((.((((((((((.(((............(((((((..........(((((((((((....))..))))))))..).........))))))).....))).)))))))))).))))).....))))...

Energy: -37.84 Kcal/mol

Graph:

Page 9: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m0458 ACAGGTCGAACGAAACGTTGAGGTATATGTGGACTACATGGTTGTCAAGTCTCCAAGCATACCCCAACATGTGGCAGACCTAG ..(((((...((....((((.(((((.(.(((((((((....)))..))))..)).).))))).))))...))...)))))..

Energy: -21.20 Kcal/mol

Graph:

Page 10: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m0523 TGTTCCTTTTCTTGCTTCTACTCACCCCCATGACCATTAGTTTATACTTAGTCCAAGTTGTACTTTATTTTGCTGTGGAAGAAGAATAAGACCAAGGTTTTAATTAGAGCCTCGTTTGTGCAAGTGAAAAATAGGATCACATTGACCTATTCTTTTTTCAAGCTCTTTTAATTGTCTTGGGAAGAAAATGAACCCAGAAATGAATGGGGTTGAGGCTGAGAAGAGGCAAGAGAATGGACA (((((.(((((((((((((.((((.((((((......(.((((((......((((((..............(((.(((((((((((((..((..(((((((....))))))).)).....(((((((.........)))).)))...))))))))))))))))............))))))......)))))).........))))))).......)))).))))))))))))).)))))

Energy: -61.27 Kcal/mol

Graph:

Page 11: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m0550 TGGGTGCTCTTGGAGGACCTTTGAAGGTGCAATTTGATACACAAATCGGACACCATTAAACTCACCATTAAGACAGAGACCTTGTAGTGTTGACACAAGGGAACAGATCCT ...................(((((.(((((.(((((.....))))).)..)))).)))))...................((((((.((....))))))))...........

Energy: -15.30 Kcal/mol

Graph:

Page 12: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m0590 CTGGAAAGACTATTCTGGATTCAAATTGTTACTTTTAGAATAGATTTTTCGTAAGTGCAATACTGATCTTCGGAACCCCCTTTCTA .(((((((....(((((((.(((.(((((.((((...(((.......)))..)))))))))..)))..)))))))....)))))))

Energy: -19.50 Kcal/mol

Graph:

Page 13: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m0626 TTTATCTCCTTCGTCGGCATCATTGACTTGACATATTTGCATATAGAGTATTGTACAGGATTAAAAAAATAAATTTAATGGCATTTTCAACGGCCAAAAGCATGATTCAACTTAAGACCATGAAGGGAATATAATGTGATGAGGGAAATGAAGACGACGAAGTAGTCAAA .....((.((((((((.(.(((((..((((.((((((...............................................................................................)))))).))))...))))).).)))))))).)).....

Energy: -21.07 Kcal/mol

Graph:

Page 14: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m0704 GGATATTTCTTTAGGACTATGGTTTGGACGAGTCACTACTCAGCAGAGAACATGCTAATTGATTTGTGTGCACCGCAAAAGGGGAATAAGATTTTCTACCTATGGCGAGGTATATGTCTGTTGATTTTGGTTATGTTCTCATAACTTTTATTGTTTTCAGCTTTTAGTCCAAAATCGTGCAGAGCTTGATAACACAGAAGAGTTCAGTTGCCACTTGTCCAATCCATAGTTTTAAAGGAATGAACCC ((.(((((((((((((((((((.((((((((((..(.(((.....(((((((((((((((((.....((((..(((.(.(((((((.......)))).))).).)))..)))).......)))))..)))).))))))))..(((((((..((((.((((((((................)))))).)).))))...))))))).))).)..)))))))))).)))))))))))))))))))..)).

Energy: -76.99 Kcal/mol

Graph:

Page 15: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m0719 TATTTCCTTTTACTTGGTGAATTGTTGGATCACTCTGTGTCAACCAACCATATTGGATTTGGAGCTTCATGCAGAAGACAAAAGAGATGCAAGCCAGAGGAAATATAGAATGATCCAACATTTCACTATGTAAAAGGATTGC ....(((((((((.(((((((.((((((((((.(((((((...(((.......)))........((((.....)))).........................))))))).)))))))))).))))))).)))))))))....

Energy: -48.20 Kcal/mol

Graph:

Page 16: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m0723 TTTAGGGACTCCTGTCGTAGGAGAGATGACGCCTTCTGTTAGGTAATTAATATGTTGCAAAGCCAACTTGAATTAGCAGTAGCTGTCATCTTTCCTCGGCTGAAGCCCCTTCA ...((((.((.(.((((.(((((((((((((.((.(((((((...........((((......)))).....))))))).)).))))))))))))))))).).)).))))...

Energy: -41.79 Kcal/mol

Graph:

Page 17: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m0877 AGAGGAGGCATCCAAAGGGATCACATTGATCCCAAATCCAAGCTTCAATGTTCTTCTCTCTTTCTCACTCAAAATATTAATTTTATTTGGGATCATGCTATCCCTTTGGACTTCTC .((((((...(((((((((((..((.(((((((((((...............................................)))))))))))))..)))))))))))))))))

Energy: -36.93 Kcal/mol

Graph:

Page 18: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m0904 GGGAGAAGAGCTTGAGGAAGTGATGGGAGATGGGAGGGTCGGTAAAGAATATATCTGAGACTCGACTCAATCTCGATCTCTCTCAGTGTTGTGTTGTTTTGTTTATCCTTTTGCCGATTCCACCCATTCCTATGATTTCCTTCGGTTCCTCTCTTTC ((((((.(((((.((((((((.((((((.(((((.((((((((((((.(((...((((((.((((.......))))....)))))).................))).).))))))))).)).))))))))))).)))))))).))))).))))))..

Energy: -60.45 Kcal/mol

Graph:

Page 19: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m1008 CCCTGGGTGATTCAGAAGTTGTTAAGGAATTTACAAGGATAGTGGAAATCTCAAGTGGATTGCTTAAGGACTGGACGTAGG ................((((.(((((.(((((((..((((.......))))...))))))).))))).)))).........

Energy: -16.90 Kcal/mol

Graph:

Page 20: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m1049 TCATTTTTCTGTGGTCACTTCTGCTCTGGGTGAGAGAAACACGTATTGAAGAAGGTTCATGGCTTAGACAATAAGTTGCTACTTTGCTGAACTCACCATTTTATGTGAAGAAGTGGCTATGCTAATTGTTTGATTCTGTACACGTACGATACGGGTCGCTCTCACCTGGAGTAGGGCTGCCCAAAGCTTTGATAAA ........((.(((.((.((((((((((((((((((..((.(((((((......((.((.((.(((((((((.(((.((((((((.......((((........)))).))))))))...))).))))))))).)))).)).....))))))).))..)))))))))))))))))).)).))).))..........

Energy: -70.10 Kcal/mol

Graph:

Page 21: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m1063 GTTTTTTTTCTTTTGTGTCGTGAAGCTTTTGATGCCTAAGAACCCTAGAGCACCAATTATTAGATGTAGTATTAGTGGCCAAGCACAAAAAAAAAAAAAA .((((((((.((((((((...(..(((...(((((.................................)))))...))))..)))))))).)))))))).

Energy: -12.81 Kcal/mol

Graph:

Page 22: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m1071 GTCGCAAGCAAGAATGTGAGTTAGAGTGAGCATCCCTAAAAAAAGAATTTCAAATTCTATATTTTTGAGAGAATTTGAAATCCCACTATTTTAGTCTGTCGCAACCTACCTTACGACG ...............(((((.(((......((...((((((...(.((((((((((((.(.......).)))))))))))).).....))))))..)).......))).)))))....

Energy: -16.12 Kcal/mol

Graph:

Page 23: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m1090 GATTTTCTTATTTTTAAAAGGTTCAGTTAGGTCCTTTATTTTTTAAAATGGGTTCAATTTGGTCCCACTTAAACTCATTTTTTGAAAATGTTCGAAGCTGTTGGAAGAAAATGGGACCAACCCAATTTAAAAAATAAAAGACCTAATTGAACCTTTTAAAAATAAAGGGACCA ....(((((((((((((((((((((((((((((.(((((((((((((.(((((......((((((((............(((((((....)))))))..............))))))))))))).))))))))))))).)))))))))))))))))))))))))).)))....

Energy: -68.37 Kcal/mol

Graph:

Page 24: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m1107 ACGAGTTTGAGCTGGCGTCGACACGTGGCATGGGACTAGTCAGTTAAACTGGCGATTTGTAAGTAGCAGGGCTCCTCAGCTTTTTCAGGGTTGCAGCTCAGCTTTTTCAGGGTTGTAGGCACGCGTGAAGGCAGCAGGAGCAGCACTTGA ...(((((.((((((((((..((.......)).)))..))))))))))))...............((...(((((((.((((((.(.(.((((((((((.(......).))))))).))).)..).)))))).).)))))).))......

Energy: -37.70 Kcal/mol

Graph:

Page 25: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m1128 ATCTTTGATCTGGGTGAGAGAAACGCGTATCGATGAGATTCAGGGCTTACTAGTTCACACAGTTGCATCCACACACCAATTGTTTTAACAATTTGGAGTGAGTGTGTTTTGATCGATACGGGTCCTCTCACCTAGACTAGAGTT ..(((((.((((((((((((..((.(((((((((........(((((....))))).........((..(.(((.((((((((....))))).))).))).)..)).....))))))))).)).)))))))))))).)))))..

Energy: -54.40 Kcal/mol

Graph:

Page 26: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/Fold: >soy-m1167 TGATCATGGCAATGTCTGGGCTTAGCGAGGCGGTCACGAGCTTAGCGTGCTTTTGGGTTTCTTCATGGTCCTTCTTCGTGCTAAGCTTGAGCTTGCCTGCTAAGCGAGGAGGTGCGTTGAGCC ........((....(((..(((((((.((((((.(.(((((((((((((.....(((((.......))))).....))))))))))))).).)))))))))))))..)))...))........

Energy: -42.80 Kcal/mol

Graph:

back

Page 27: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m11AAATTCTG........

Energ-28.90 K

Graph

Fold: 191 GGAAGTAGTC....(((((.

gy: Kcal/mol

h:

CTTTCCTTTTT....((.....

TGGTAAACTA.))...))))

ACACTTTCCCT).......((

TCTTCAAAAG((.(((((((

GTTCTTATGGA(..(((((((.

AAAAGACACA...(((((..

ATAAAGATGTC......))))

CTGTTTGCAT)).((((...

ACAAGCCATA.)))))))))

AAGCTCTTTT)))..)))))

GAGAGCTTCT))))).....

TCTAGAGTAT..........

T

Page 28: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m12GCTTTGAA..((((((

Energ-21.19 K

Graph

Fold: 200 AGAAGTGGGT((..(((.((

gy: Kcal/mol

h:

TGGGTTGGTGC((((((((..(

CTGCAGCTAG((...(((((

GATCAAAATGG((((.......

GGATTTAGCG.)))))))).

GAATTGGGAAT..........

TCGGAGATAA..........

AGAGAAAGCCA.....)))).

ATGACTTACA..))))))).

ACACGCATTTC)))...))))

CAAATG ))))..

Page 29: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m12CATCGAGAGAAAATTT((((....)...))))

Energ-58.71 K

Graph

Fold: 212 ATTTTATAGTTAGATTTCAA.((((((((())))))))).

gy: Kcal/mol

h:

TCTGACATCTGAATGTCAGATT((((((((.((.))))))))))

GGAATTTAAATATAAAACAT(((((((((()))))))...

ATGTCTCAACATGGGTGA ((...((((((.)))).

AAGGGCGTTA

((((((....

AGATCCAACAA

..........

ACGTTCCACA

..........

AATGCTAATGG

..((....((

GGAGAATTCA

((((((....

AAATTTGGGAC

(((..((((.

CCTCTCTCTC

....))))..

ATTTCTTCTC

)))..)))))

CCTTTCACGCT

))).....)).

CTTTGAAACAA

..........

AATAAAGGAC

..........

CACCCGAGATC

..........

CCCCGTAGAA

..........

GCTCTTGTTG

))))))))))

G

Page 30: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m12GTGGATTGCGTCAATA((((((((........

Energ-86.36 K

Graph

Fold: 249 GTTAACTTGGATAGAATCCC(((.((((((.........)

gy: Kcal/mol

h:

GGCTGAGCTTACGGTCAACACC((.(((((((.))))))))).

AGGTGAGTGTCCACCGGAGT.((((.((((.)))).))))

TTGACCGTGGTTTCAATTCAAG((((((((((())).))))))

TGTGCTCTAAGTCAATAGTT(((((((((()).)))))))

AGCAAACCTAGTCACC (....((((..))).

GATTCAAGGT

......))))

TCTTTGGAGTT

..)))))))(

TCAGCTTACC

(((((((.((

CTCTGGCAAG

....))..))

GGTTGAGACA

)))))).)))

CCATGGGGGA

)))((((((.

ATGGGTGTGTA

.(((((((...

TACCTGCAAAA

..........

AAAGACACTC

....))))))

CAGACTCTCAG

)..)))))).

GTCATTAAGG

..........

AATCTACCAG

..........

G

Page 31: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m12GCTGATGCCACGCACA.(((((((........

Energ-75.21 K

Graph

Fold: 250 CTAGGTGCACACCTAGCACA((((((((((..........

gy: Kcal/mol

h:

CCAGCATTATTAAATGTGAAGA(((((((((((...........

TGCTGGTGCAAAAGTGTACT((((((((((...)))))))

ATCCAGCACTTTAGCAATAATG((..((((...))))))))))

TAAAGAGAAAGTTGGATGCA.......((())))).))))

AAGACTAAAATACCTAGCATCA((((((.....))))))))))

TATCTCTGATAG .((.....))))

TTCATAAGTCT

.....)))))

TTTTACGGAT

))))......

CAAATGCTTA

....))))((

AATATCAACA

(((((.....

TACGAATCAA

..........

ACTTAATTTAT

.........))

ATATTAATCTT

))))).....

TACGGATCAA

...(((((..

ACTCGATCCAT

...)))))..

TACAATTTAC

..........

GGATCACTCT

..........

T

Page 32: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m13GCATCAAC...(((.(

Energ-77.60 K

Graph

Fold: 316 CTTCGACGAA(((((((((.

gy: Kcal/mol

h:

ACTCTTCGTCG..(((((((((

GGCATCGACA((..(.((((

ATCCACGGAGA(((((((((((

ATGTCTTGCC(...((((((

CGGACTTGGAG(((((......

GATGTTCGGC...)))))))

CGAGTTCTCCG))).))))))

GTGGATGTCA))))))))).

AGCACCGGCGA)..)))))))

AGGAATCGTC)))).)))))

GGAGATGAAC)))).)))..

CT ..

Page 33: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m13GCTTCTAATTGGAGTT..........)))).)

Energ-17.07 K

Graph

Fold: 336 AGCCAAGTCCTAGCGGGAAA..........).))))....

gy: Kcal/mol

h:

CAAAGTAGGAAAGCA ...(((..(((....

ATGTTGCATT

((((((....

TTTCAAACAAT

(((.......

TTTGTTAGCA

..........

ATTGGAAAACA

..)))...))

AGTATTCTAT

))))))).))

TTTTTGAGTTG

).........

GCCAAATAGA

..........

ACCATGTTAAA

..........

AGCCAACCAC

..........

CAATACTGCC

.....((((.

CATCTCTTCAT

.(.((((.(((

ATCCTTATGCC

((...(..((

CTTCAGCCTG

(((((((.((

GAATATGTGTA

........))

ATGTTGAAGG

).))))))))

AAATGATGTT

...)))))..

T

Page 34: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m13TTTTGGTC.((((.((

Energ-65.43 K

Graph

Fold: 354 CTGGGTTCCT((((((((((

gy: Kcal/mol

h:

TATTGTCAAGT(((((((.(((

TTAAGTGATG((((((((((

GACATGACATC((..(((((((

CATCTGATGA(((((((((.

ACTGATATATA(.........

ATTGGAAGAG.........)

GCCGTTGAGAT.)))).))))

TGATGTCACA))))))))..

ATCATCACTTA))))))))))

AGCTGACGGT))))))))))

AGGGACCTAG))))))))))

GGACAAA )).))))

Page 35: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m13CCAACTTC......((

Energ-19.10 K

Graph

Fold: 360 CAGGCTCAGG(((..((((.

gy: Kcal/mol

h:

GAGGCAGTGTG...((((((((

GAGTTCTTGT((..((....

TGATTGCATCT))))))).))

TGCGACTGAA)))..)))).

AACTGAGACTG.)))).....

GCATCTGGAT..........

TGAAGGATA ........

Page 36: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m14GGTAAATT.(((((.(

Energ-55.60 K

Graph

Fold: 403 TGAATTGAAC((((((((((

gy: Kcal/mol

h:

CTTCTCCTATA((((((((((.

AAGTTAAAAT.(((((((((

TCAACTTATAC(((..((((((

CACATAATTT(((....(((

TTCGTAGAAAC((.(.((((.(

CTCTATCCTA((........

ATAGCTTCTTC.)).)))).)

CAAAAGCTGA).))))....

AAGTGTGTAAC.)))))))).

CCTGATTTTA..))))))))

GCTGGTAGGA))).))))))

AGAAGTTCAGT)))))))))))

GTTCATTTTAC)))).)))))

C )

Page 37: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m14GGCCGCGG.((.((((

Energ-63.50 K

Graph

Fold: 418 GTGGCGGGTC((((((.(((

gy: Kcal/mol

h:

CCCTCCGGGTC(((((((.(((

CGTCTCCGTC((((((((((

CTGCGGAGGAC(..........

CGACGGAGAC.)))))))))

CGACGACGGGG)))..)))))

GAGGACGCGC).)))).)))

CCGCTGCTGCA)))))).)).

A .

Page 38: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m14GTTGGAAC.(((((((

Energ-43.60 K

Graph

Fold: 427 CAGAGGAATC(.((((((((

gy: Kcal/mol

h:

CCATTCAGATC(((((.(((((

CTGTTTCGCA((((((((..

AACACCATATT..(((.....

TGGGTTCGAC..))).....

CTGAAACAGAT))))))))))

TCTAAATGGA))).))))))

ATTCCTTAGTT)))))).)))

TCTGA )))))

Page 39: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m14GGATATTTCTGCAGAG((.(((((....))))

Energ-66.17 K

Graph

Fold: 444 TCTTTAGGACGGTTGATAAC(((((((((())))).....

gy: Kcal/mol

h:

CTATGGTTTGGCACATTGTAGT((((((.((((....)))))).

GACAAGTCACTGACAGAAGA(((((((....)))))))..

CTACTCAGAGCAGTTCAGTTGC.((((((.((.....))...

CACAAAACATCCACTTGTCC((((........))))))))

TGCTAATTAATCAATCCATAGT..........)).)))))))

TTTGTGTGCATTTTAAAGGA....))))).))))))))))

ACTGGAATGTTAATGAACCC ))))......)))..)).

TCTCTACCTA

..........

ATCTATCGTGA

..........

AGCTATATAT

..........

CTCTTTGGTT

.......(((

TATGTTCTCAT

((((....)))

ATAACTTCTGT

))))((((((

TTTTTGCAAG

((.(((((((

GTAATCTTGAC

....).....

CCAGCTTCTA

.((((((((.

GTCCAAAATC

..........

C

Page 40: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m14AAACTCTT....((((

Energ-45.69 K

Graph

Fold: 480 TCATTAGCTA((((((((((

gy: Kcal/mol

h:

AATCATTAGCT(((((((((((

TAATTTGTAA(((((.(((.

AATGTCATCTC(.((..((((

CATTTTTTAG(((.......

GAAAAGCTTTA.........)

ATGAGAGCTT)))))))).)

TCTACTAATTA.))).)))))

AGCTAATGGT))))))))))

TAGCTAATGA))))))))))

AAGAACTC ))))....

Page 41: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m14ATTTACTT........

Energ-29.50 K

Graph

Fold: 498 TGTTAGTGTT....((((..

gy: Kcal/mol

h:

TGTCCTGTCGA...((((((((

AACACGGACA(.((((.(((

ACCAACATGTG((...((((((

GTCAGACACC(((.(....)

CAGACACGAAC.)))))....

CAAAAGAATG.......)))

GGAGTGTACGT..)))).)))

TGATTGATAG)).)))))))

GGTTCTCACTT)....)))).

TCTGTAGAAT..........

ATCTATTTTT..........

TACTTTCATGC...........

GCAACGTGGAT..........

TA ..

Page 42: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m15GAAAGTCT.((((.((

Energ-45.75 K

Graph

Fold: 500 TTTAGCAACC(((((((..(

gy: Kcal/mol

h:

CCTCAAAGGCT((((((((..(

TTCCACTACT((((((((((

TCCATATTTCA(((........

AGTCTAGTGA..........

AATGTCCACAA...((((...

AACATGGAGG....))))))

GAGTAGTGGAA))))))))))

ACTTTGAGGC))))))))).

CTGCTGAAGC.)))))))).

CCTTT .))))

Page 43: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m15TAAATTTT((((((((

Energ-47.90 K

Graph

Fold: 551 TCTTGAGAAT((((((((((

gy: Kcal/mol

h:

TTTGGCCTCTG(((((.(((((

GTCCATGTCT((((((.(.(

TAATTATTAAT((((((((...

TTCCAATAAT....))))))

TTGAGATGGAT))).))))))

TAGAGCCCAA))))).))))

AATTCTCAAGA))))))))))

AGAATTTGT)))))))).

Page 44: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m15CACTTGGGGTGTTGTA........(((.....

Energ-18.53 K

Graph

Fold: 577 GTTGATGATTACATTATCAC.................)))

gy: Kcal/mol

h:

TTTCAATACGACATTAGAGTC ....................

ATTTCATTTA

.........(

AAGAAGTTAGT

(((((((....

TTGCTTCTTT

..))))))).

TCAGCTTACCA

..........

AACAATGAAG

...((((..(

GCCACACAATC

(((((......

CAAGAATTAC

..........

AAGTGGCAAC

..)))))..)

CATTAACAAA

))))......

AATCCCTTTG

..........

GACAACCATTT

...........

TTTAGGAAAAA

..........

AAAACCATAC

..........

CAAGTTTATGT

....(((((.

TTTACATAAA

....))))).

GTCAATAATT

..........

T

Page 45: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m16TATTAGAA...(((((

Energ-24.50 K

Graph

Fold: 625 AAGTGAACTA(((((((.((

gy: Kcal/mol

h:

ATACAAAGACG((((.((((((

GGTTATTCCA((((......

AAAAACCGTTT..))))))))

TTAGTATAAT)).))))).)

TTTACTTTTTA))))))))))

ACGA )...

Page 46: Supplemental data file for Zhai et al. Predicted secondary …genesdev.cshlp.org/content/suppl/2011/12/07/25.23.2540... · 2011-12-07 · CentroidFold predicts an RNA secondary structure

Seq/F>soy-m16AAGAGTTT..((((((

Energ-108.30 K

Graph

Fold: 631 TGGGCTGGCG((((((((((

gy: Kcal/mol

h:

GTCGACACGTG(((((((((((

GGCATGAGAC((((((((((

CTAGTCAGTGG(((((((((((

GCAATTTGCA((.(((((((

AGGTAGCAGGG((.((((...(

GCTTCTCAGC(((.(((.(.

CTTTTTCAGGG.....).)))

GTAGCAGGGT).)))...))

TACTGCAAAT))))))))))

TCGCCAATTG).))).....

GACTGGCTAG.)))))))))

GTCTCATGTCA)))))))))))

CACGTGTCGAC))))))))))

CGTCAGCCCA))))))))))

AAACTCGT )))))..