Supplemental Figure 1. Mutational analysis of FL Igs ... · Framework regions (FWR1, FWR2, FWR3, and FWR4) and complementarity determining regions (CDR1, CDR2, and CDR3) are indicated

! 1!

Supplemental Figure 1. Mutational analysis of FL Igs. Variable region sequences

from the expressed Ig of tumors from 467 FL patients were cloned and analyzed. (A) The

number of mutations compared to closest match germline sequence for the heavy and

light chain V genes. (B) The frequency of mutations in the heavy and light chain V genes

calculated from the number of replacement (R; black bar) and silent (S; white bar)

nucleotides exchanges per base pair in framework regions (FWR1, FWR2, and FWR3)

and complementarity determining regions (CDR1 and CDR2). The R/S ratio for each

region is indicated.

Supplemental Figure 2. LC-MS/MS identification of the 236kD protein

immunoprecipitated by tumor Ig 1152. 236 kDA protein band peptide sequences

matching myoferlin are shown in bold and are underlined.

Supplemental Figure 3. Nucleotide sequence alignment of molecular clones of IgH

variable region from the follicular lymphoma tumor of patient 1152. Unique

nucleotide sequences and number of times they were observed are indicated by alphabetic

and numeric characters, respectively. Grey-shaded nucleotides indicate nucleotides

present in the dominant clone, whereas mutations are specified in white. Silent and

replacement mutations are represented by circles and squares, respectively. Framework

regions (FWR1, FWR2, FWR3, and FWR4) and complementarity determining regions

(CDR1, CDR2, and CDR3) are indicated.

! 2!

Supplemental Figure 4. Nucleotide sequences of IgΗ and Igλ of variable regions

from subclones derived from tumor 1152. Grey-shaded nucleotides indicate the

consensus sequence of the (A) IgΗ and (B, C) Igλ variable regions. Framework regions

(FWR1, FWR2, FWR3, and FWR4) and complementarity determining regions (CDR1,

CDR2, and CDR3) are indicated. (D) Graphical summary of mutations in both IgH and

Igλ differentiating the tumor subclones. 2E12, 6C12, 4B11, 1G2, and 1E9 were obtained

through rescue fusion of cells from the tumor biopsy of patient 1152; 1152 corresponds to

the recombinant tumor Ig from patient 1152. HN represents the hypothetical mature naïve

pre-malignant clone, based on the closest matched variable region germline genes. λRE

represents a predicted precursor that has undergone receptor editing of the light chain.

Number of mutations is indicated adjacent to connecting lines with number of

replacement mutations specified in parentheses. In bold are the locations of specific

replacement mutations; amino acid changes are indicated in parentheses.

Supplemental Figure 5. Igs from non-tumor B cells of patient 1152 exhibit different

HEP-2 staining and do not bind myoferlin. Two clones not originating from the tumor

were recovered in the rescue fusion of the tumor biopsy of patient 1152. (A) HEp-2 IFA

staining pattern of non-tumor Igs. Bars, 25µm. (B) Immunoblot for myoferlin

immunoprecipitations from lysate of 293T cells transfected with recombinant myoferlin-

HA construct by non-tumor Igs (NT1 and NT2) and Ig from tumor clone 6C12.

Supplemental Figure 6. Phenotypic Analysis of patient 1152 tumor biopsy. A single

cell suspension of the tumor biopsy of patient 1152 was analyzed by flow cytometry to

! 3!

characterize the tumor cell phenotype. FSC-A vs SSC-A was used to gate on singlets

followed by a live cell gate based on SSC-A vs FSC-A. CD3-CD20+ B cells were then

gated on and used in the analysis of tumor cell phenotype. In the two right-most columns,

plots are gated on CD3-CD20+ cells and the CD20 axis has been cropped and enlarged to

highlight differences between the CD20hi and CD20int populations. Tumor cells are IgGλ

and predominantly reside in the CD20hi population.

!

A

Supplemental Figure 1

Light ChainHeavy Chain

B

Heavy

Chain L

ight

Chain

0

20

40

60

80

100# o

f V

Muta

tions

Replacement

Silent

Fre

quency (

%)

0

5

10

15

20

25

R/S 2.0 1.2 4.1 1.7 4.3 1.7

tota

l

FW

R1

CD

R1

FW

R2

CD

R2

FW

R3

Fre

quency (

%)

0

5

10

15

20

R/S 1.7 2.1 4.4 1.0 3.7 1.5

tota

l

FW

R1

CD

R1

FW

R2

CD

R2

FW

R3

1 MLRVIVESAS NIPKTKFGKP DPIVSVIFKD EKKKTKKVDN ELNPVWNEIL

51 EFDLRGIPLD FSSSLGIIVK DFETIGQNKL IGTATVALKD LTGDQSRSLP

101 YKLISLLNEK GQDTGATIDL VIGYDPPSAP HPNDLSGPSV PGMGGDGEED

151 EGDEDRLDNA VRGPGPKGPV GTVSEAQLAR RLTKVKNSRR MLSNKPQDFQ

201 IRVRVIEGRQ LSGNNIRPVV KVHVCGQTHR TRIKRGNNPF FDELFFYNVN

251 MTPSELMDEI ISIRVYNSHS LRADCLMGEF KIDVGFVYDE PGHAVMRKWL

301 LLNDPEDTSS GSKGYMKVSM FVLGTGDEPP PERRDRDNDS DDVESNLLLP

351 AGIALRWVTF LLKIYRAEDI PQMDDAFSQT VKEIFGGNAD KKNLVDPFVE

401 VSFAGKKVCT NIIEKNANPE WNQVVNLQIK FPSVCEKIKL TIYDWDRLTK

451 NDVVGTTYLH LSKIAASGGE VEDFSSSGTG AASYTVNTGE TEVGFVPTFG

501 PCYLNLYGSP REYTGFPDPY DELNTGKGEG VAYRGRILVE LATFLEKTPP

551 DKKLEPISND DLLVVEKYQR RRKYSLSAVF HSATMLQDVG EAIQFEVSIG

601 NYGNKFDTTC KPLASTTQYS RAVFDGNYYY YLPWAHTKPV VTLTSYWEDI

651 SHRLDAVNTL LAMAERLQTN IEALKSGIQG KIPANQLAEL WLKLIDEVIE

701 DTRYTLPLTE GKANVTVLDT QIRKLRSRSL SQIHEAAVRM RSEATDVKST

751 LAEIEDWLDK LMQLTEEPQN SMPDIIIWMI RGEKRLAYAR IPAHQVLYST

801 SGENASGKYC GKTQTIFLKY PQEKNNGPKV PVELRVNIWL GLSAVEKKFN

851 SFAEGTFTVF AEMYENQALM FGKWGTSGLV GRHKFSDVTG KIKLKREFFL

901 PPKGWEWEGE WIVDPERSLL TEADAGHTEF TDEVYQNESR YPGGDWKPAE

951 DTYTDANGDK AASPSELTCP PGWEWEDDAW SYDINRAVDE KGWEYGITIP

1001 PDHKPKSWVA AEKMYHTHRR RRLVRKRKKD LTQTASSTAR AMEELQDQEG

1051 WEYASLIGWK FHWKQRSSDT FRRRRWRRKM APSETHGAAA IFKLEGALGA

1101 DTTEDGDEKS LEKQKHSATT VFGANTPIVS CNFDRVYIYH LRCYVYQARN

1151 LLALDKDSFS DPYAHICFLH RSKTTEIIHS TLNPTWDQTI IFDEVEIYGE

1201 PQTVLQNPPK VIMELFDNDQ VGKDEFLGRS IFSPVVKLNS EMDITPKLLW

1251 HPVMNGDKAC GDVLVTAELI LRGKDGSNLP ILPPQRAPNL YMVPQGIRPV

1301 VQLTAIEILA WGLRNMKNFQ MASITSPSLV VECGGERVES VVIKNLKKTP

1351 NFPSSVLFMK VFLPKEELYM PPLVIKVIDH RQFGRKPVVG QCTIERLDRF

1401 RCDPYAGKED IVPQLKASLL SAPPCRDIVI EMEDTKPLLA SKLTEKEEEI

1451 VDWWSKFYAS SGEHEKCGQY IQKGYSKLKI YNCELENVAE FEGLTDFSDT

1501 FKLYRGKSDE NEDPSVVGEF KGSFRIYPLP DDPSVPAPPR QFRELPDSVP

1551 QECTVRIYIV RGLELQPQDN NGLCDPYIKI TLGKKVIEDR DHYIPNTLNP

1601 VFGRMYELSC YLPQEKDLKI SVYDYDTFTR DEKVGETIID LENRFLSRFG

1651 SHCGIPEEYC VSGVNTWRDQ LRPTQLLQNV ARFKGFPQPI LSEDGSRIRY

1701 GGRDYSLDEF EANKILHQHL GAPEERLALH ILRTQGLVPE HVETRTLHST

1751 FQPNISQGKL QMWVDVFPKS LGPPGPPFNI TPRKAKKYYL RVIIWNTKDV

1801 ILDEKSITGE EMSDIYVKGW IPGNEENKQK TDVHYRSLDG EGNFNWRFVF

1851 PFDYLPAEQL CIVAKKEHFW SIDQTEFRIP PRLIIQIWDN DKFSLDDYLG

1901 FLELDLRHTI IPAKSPEKCR LDMIPDLKAM NPLKAKTASL FEQKSMKGWW

1951 PCYAEKDGAR VMAGKVEMTL EILNEKEADE RPAGKGRDEP NMNPKLDLPN

2001 RPETSFLWFT NPCKTMKFIV WRRFKWVIIG LLFLLILLLF VAVLLYSLPN

2051 YLSMKIVKPN V


A {27}B (7)C (2)D (1)E (1)F (1)G (1)H (1)I (1)J (1)K (1)L (1)M (1)N (1)O (1)P (1)Q (1)R (1)S (1)

10 20 30 40 50 60 70C A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A G C C G C A C A G A C C C T C A C A C T G A C G T G C A C T T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T C A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G A T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A G C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C T


80 90 100 110 120 130 140 150G G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G A T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G A T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G A T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T T


160 170 180 190 200 210 220G G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G T C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C A A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C C


230 240 250 260 270 280 290 300G C C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A A T AG C C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A A T AG C C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A T T G T G C C C G A A T AG C C A A A A A T C A A G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A A T AG C C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A T A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A G T AG C C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A A T AG C C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A A T AG C C A A A A A T C A A G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G C A T AG C C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A A T AG T C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A A T AG C C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A A T AG C C A A A A A T C A G G T A G T C C T C A C A G T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A A T AG C C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A G T AG C C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A G T AG C C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A C A T G G A G C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G T A T AG C C A A A A A T C A G G T A G T C C T C A C A A A A A C G A A T A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A A T AG C C A A A A A T C A G G T A G T C C T C A C A C T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A A T AG C C A A A A A T C A G G T A G T C C T C A C A A T G A C G A A C G T G G A C C C T G T G G A C A C A G G C A C A T A T T A C T G T G C C C G A A T AG C C A A A A A T C A G A T A G T C C T C A C A A T A A C G G A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G T C C G A A T A


310 320 330 340 350 360G C G G T G A C C T T T G C G G G A C T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C T T T T G C G G G A G T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C T T T T G C G G G A G T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C C T T T G C G G G A C T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C T T T T G C G G G A A T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C C T T T G C G G G A G T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C C T T T G C G G G A C T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C C T T T G C G G G A G T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C T T T T G C G G G A G T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C C T T T G C G G G A C T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C T T T T G C G G G A G T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C C T T T G C G G G A C T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C T T T T G C G G G A G T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C T T T T G C G G G A G T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C T T T T G C G G G A G T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C C T T T G C G G G A C T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C T T T T G C G G G A G T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C T T T T G C G G G A G T T A C C A C A G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C C T T T G C G G G A G T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C A

FWR1

CDR1 FWR2

CDR2 FWR3

CDR3

FRW4


Germline1152Clone 6C12Clone 4B11Clone 1G2Clone 1E9Clone 2E12

10 20 30 40 50 60 70C A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T G A A A C C C A C A C A G A C C C T C A C A C T G A C C T G C A C C T T C T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A G C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C T G G T A A A A C C C G C A C A G A C C C T C A C A C T G A C G T G C A C C T T T T C TC A G G T C A C C T T G A G G G A G T C T G G T C C T G C G C A G G T G A A T C C C A C A C A G A C C C T C A C A C T G A C C T G C A C C T T C T C T


80 90 100 110 120 130 140 150G G G T T C T C A C T C A G C A C T A G T G G A A T G T G T G T G A G C T G G A T C C G T C A G C C C C C A G G G A A G G C C C T G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G G T T T T C A C T C T C C A C T C G T G G A A T G T G T G T C T C C T G G A T C C G T C A G T C C C C A G G G A A G G C C C C G G A G T G G C T TG G C T T C T C A C T C A C A A C T A A G G G A A T G T G T G T A A T T T G G A T C C G T C A G C C C C C A G G G A G G G C C C T G G A G T G G C T T


160 170 180 190 200 210 220G C A C T C A T T G A T T G G G A T G A T G A T A A A T A C T A C A G C A C A T C T C T G A A G A C C A G G C T C A C C A T C T C C A A G G A C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG G A C G C A T T G A C T G G G A T G A T G A T A A A T T C T A T A G T G C C T C T C T G A A G A G G A G A C T C A C C A T C T C C A A G G G C A C CG C G C G C A T T G A T T G G G A T G A T G A G A A G T A C T A T A G A A C A T C T C T G A A G A C C A G A C T C A C C A T C T C C A A G G A C A A C


230 240 250 260 270 280 290 300T C C A A A A A C C A G G T G G T C C T T A C A A T G A C C A A C A T G G A C C C T G T G G A C A C A G C C A C G T A T T A C T G T G C A C G G A T AG C C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A A T AG C C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A A T AG C C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A A T AG C C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G T G C C C G A A T AG C C A A A A A T C A G G T A G T C C T C A C A A T A A C G A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T T C T G T G C C C G A A T AT C C A G A A A G C A A G T G G T C C T T A C A G T G G C C A A C A T G G A C C C T G T G G A C A C A G C C A C A T A T T A C T G C G C A C G A C T A


310 320 330 340 350 360 370G C G G T G A C G T T T G G G G G A G T T A C C A C C G T C G A C T A C T G G G G C C A G G G A A C C C T G G T C A C C G T C T C C T C AG C G G T G A C C T T T G C G G G A G T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C T T T T G C G G G A G T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C C T T T G C G G G A C T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C C T T T G C G G G A C T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AG C G G T G A C C T T T G C G G G A C T T A C C A C C G T C G A C T T T T G G G G C C A G G G A G C C C T G G T C A C C G T C T C C T C AA G G G T T T C A T T T G C G G G A G A T C C C G T C G T C G A T T A T T G G G G C C C G G G A A C C C T G G T C A C C G T C T C C T C A

FWR1

CDR1 FWR2

CDR2 FWR3

CDR3

FRW4

A

B


HN

2E12

IgH:VH2-70, DH3-16, JH4

Igh:Vh9-49, Jh3

IgH: 33(22)

Igh:44(24)

IgH: 8(3)

hRE

Igh:Vh8-61, Jh7

?

IgH: 31(14)

Igh:30(14)1152 6C12

IgH: 1(0)

Igh:4(2)

4B11

IgH: 1(1)

hFWR2 (R H)

hFWR4 (Q H)

NFWR3 (Y F)

1G2

IgH: 1(0)

1E9

IgH: 1(1)

Igh:2(0)

NCDR3 (V L)

C

FWR1

CDR1 FWR2

CDR2 FWR3

CDR3

FRW4

Germline1152Clone 6C12Clone 4B11Clone 1G2Clone 1E9

10 20 30 40 50 60 70C A G A C T G T G G T G A C C C A G G A G C C A T C G T T C T C A G T G T C C C C T G G A G G G A C A G T C A C A C T C A C T T G T G G C T T G A G CC A G A C T G T G G T G A C C C A G G A A C C A T C G G T C T C A G T G T C C C C T G G A G G G A C A G T C A C A G T C A C T T G T G G C T T G A C GC A G A C T G T G G T G A C C C A G G A A C C A T C G G T G T C A G T G T C C C C T G G A G G G A C A G T C A C A G T C A C T T G T G G C T T G A C GC A G A C T G T G G T G A C C C A G G A A C C A T C G G T C T C A G T G T C C C C T G G A G G G A C A G T C A C A G T C A C T T G T G G C T T G A C GC A G A C T G T G G T G A C C C A G G A A C C A T C G G T C T C A G T G T C C C C T G G A G G G A C A G T C A C A G T C A C T T G T G G C T T G A C GC A G A C T G T G G T G A C C C A G G A A C C T T C G G T C T C A G T G T C C C C T G G A G G G A C A G T C A C A G T C A C T T G T G G C T T G A C G


80 90 100 110 120 130 140 150T C T G G C T C A G T C T C T A C T A G T T A C T A C C C C A G C T G G T A C C A G C A G A C C C C A G G C C A G G C T C C A C G C A C G C T C A T CT C G G G C T C A G T C T C T A C T T C T A A T T A T C C C A G T T G G T A C C A A C A G A C C C C A G G C C A G G C T C C A C G C A C T C T C A T TT C G G G C T C A G T C T C T A C T T C T A A T T A T C C C A G T T G G T A C C A A C A G A C C C C A G G C C A G G C T C C A C A C A C T C T C A T TT C G G G C T C A G T C T C T A C T T C T A A T T A T C C C A G T T G G T A C C A A C A G A C C C C A G G C C A G G C T C C A C G C A C T C T C A T TT C G G G C T C A G T C T C T A C T T C T A A T T A T C C C A G T T G G T A C C A A C A G A C C C C A G G C C A G G C T C C A C G C A C T C T C A T TT C G G G C T C A G T C T C T A C T T C T A A T T A T C C C A G T T G G T A C C A G C A G A C C C C A G G C C A G G C T C C A C G C A C T C T C A T T


160 170 180 190 200 210 220T A C A G C A C A A A C A C T C G C T C T T C T G G G G T C C C T G A T C G C T T C T C T G G C T C C A T C C T T G G G A A C A A A G C T G C C C T CT T C A A C A C T A A C A C T C G C T C T T C T G G G G T C C C T G A T C G C T T C T C T G G C T C C A T C C T T G G G A C C A A A G C T G C C C T TT T C A A C A C T A A C A C T C G C T C T T C T G G G G T C C C T G A T C G C T T C T C T G G C T C C A T C C T T G G G A C C A A A G C T G C C C T CT T C A A C A C T A A C A C T C G C T C T T C T G G G G T C C C T G A T C G C T T C T C T G G C T C C A T C C T T G G G A C C A A A G C T G C C C T TT T C A A C A C T A A C A C T C G C T C T T C T G G G G T C C C T G A T C G C T T C T C T G G C T C C A T C C T T G G G A C C A A A G C T G C C C T TT T C A A C A C T A A C A C T C G C T C T T C T G G G G T C C C T G A T C G C T T C T C T G G C T C C A T C C T T G G G A C C A A A G C T G C C C T T


230 240 250 260 270 280 290 300A C C A T C A C G G G G G C C C A G G C A G A T G A T G A A T C T G A T T A T T A C T G T G T G C T G T A T A T G G G T A G T G G C A T T T C T G T GA C C A T C A C G G G G G C C C A G G C A G A A G A T G A A T C C G A A T A T T A T T G T T T A T T G T A T A T G G G C A G T G G C A C T T C T G T GA C C A T C A C G G G G G C C C A G G C A G A A G A T G A A T C C G A A T A T T A T T G T T T A T T G T A T A T G G G C A G T G G C A C T T C T G T GA C C A T C A C G G G G G C C C A G G C A G A A G A T G A A T C C G A A T A T T A T T G T T T A T T G T A T A T G G G C A G T G G C A C T T C T G T GA C C A T C A C G G G G G C C C A G G C A G A A G A T G A A T C C G A A T A T T A T T G T T T A T T G T A T A T G G G C A G T G G C A C T T C T G T GA C C A T C A C G G G G G C C C A G G C A G A A G A T G A A T C C G A A T A T T A T T G T T T A T T G T A T A T G G G C A G T G G C A C T T C T G T G


310 320 330T T C G G A G G A G G C A C C C A G C T G A C C G T C C T CT T C G G A G G A G G C A C C C A G T T G A C C G T C C T AT T C G G A G G A G G C A C C C A C T T G A C C G T C C T CT T C G G A G G A G G C A C C C A G T T G A C C G T C C T CT T C G G A G G A G G C A C C C A G T T G A C C G T C C T CT T C G G A G G A G G C A C C C A G T T G A C C G T C C T C

GermlineClone 2E12

10 20 30 40 50 60 70C A G C C T G T G C T G A C T C A G C C A C C T T C T G C A T C A G C C T C C C T G G G A G C C T C G G T C A C A C T C A C C T G C A C C C T G A G CC A G C C T A T A T T G A G T C A G T C A C C T T C T A C A T C A G C T T C C C T G G G A G C C T C G G T C A C A C T C A C C T G C A C C C T G A A C

GermlineClone 2E12

80 90 100 110 120 130 140 150A G C G G C T A C A G T A A T T A T A A A G T G G A C T G G T A C C A G C A G A G A C C A G G G A A G G G C C C C C G A T T T G T G A T G C G A G T GA A C G A C T A C A G T A A T T A C A A A G T G G A C T G G T A T C A A C A G A G A C C A G G A A A G G G C C C C C G A T T T G T G A T G C G G G T G

GermlineClone 2E12

160 170 180 190 200 210 220G G C A C T G G T G G G A T T G T G G G A T C C A A G G G G G A T G G C A T C C C T G A T C G C T T C T C A G T C T T G G G C T C A G G C C T G A A TG A C A C T G G C G G A A T T G T G G G G T C G A A G G G G G A C G A C A T C C C T G A T C G C T T C T C A G T C T T G G G C T C A G G C C T G A A T

GermlineClone 2E12

230 240 250 260 270 280 290 300C G G T A C C T G A C C A T C A A G A A C A T C C A G G A A G A G G A T G A G A G T G A C T A C C A C T G T G G G G C A G A C C A T G G C A G T G G GC G T T C C C T G A C T A T C A A A A A C A T C C A A G A A G C A G A T G A G G C T G A C T A T G T T T G T G G G A C A T G T C T T G G C A G T G G G

GermlineClone 2E12

310 320 330 340A G C A A C T T C G T T G T G G T A T T C G G C G G A G G G A C C A A G C T G A C C G T C C TA G C A A C T T C G T T G T G A T T T T C G G C G G A G G G A C C A G G T T G A C C G T C C T

FWR1

CDR1 FWR2

CDR2 FWR3

CDR3

FRW4

D

A

B

non-tumor Ig 2non-tumor Ig 1


IP:

WB: _-myoferlin

6C12NT2NT1

FSC-A

FS

C-H

94%

FSC-A

SS

C-A

61%

27%

CD20

CD

3

CD20

FS

C-A

CD20

SS

C-A

CD20

IgM

CD20

IgG

CD20

Ka

pp

a

CD20

Lam

bda


Reactivity

Isoptype VH JH # of VH Mut

Variable Region N-linked Glycoylation

Sites Excluding Germline

Focused Test Selection CDR

Focused Test Selection FWR Isotype VL JL

Variable Region N-linked Glycoylation

Sites Excluding Germline

Focused Test Selection CDR

Focused Test Selection FWR Hep-2 IFA

505 IgM 3-11*03 6*03 24 2 "0.12 "0.145 κ 1-5*03 1*01 0 0.119 "0.00547 Cytoplasmic646 IgG 1-46*01 6*02 27 0 "0.452 "0.00643 κ 3-20*01 4*01 1 "0.262 "0.0146 Cytoplasmic664 1709 IgM 1-46*01 4*03 16 1 0.0946 "0.279 λ 3-25*03 3*02 0 "0.0776 "0.0000608 Cytoplasmic689 IgG 3-30*03 4*02 30 2 0.331 "0.43 κ 1-5*03 1*01 0 "0.479 "0.0381 Cytoplasmic835 IgG 3-23*04 5*01 59 0 0.0781 "0.021 κ 3-11*01 4*01 1 0.283 "0.131 Cytoplasmic855 IgG 3-30-3* 4*02 51 0 "0.249 "0.0593 λ 3-21*02 3*02 0 "0.401 "0.0205 Cytoplasmic912 IgM 3-23*04 4*02 46 1 "0.00313 "0.000123 κ 4-1*01 2*02 0 "0.279 "0.00651 Cytoplasmic950 IgG 4-4*07, 4*02 52 5 0.278 "0.0153 κ 4-1*01 5*01 0 "0.0552 "0.0125 Cytoplasmic953 IgG 5-51*01 6*02 5 0 0.37 "0.08 κ 4-1*01 2*01 0 "0.328 "0.0152 Cytoplasmic998 IgM 4-30-4* 4*02 68 1 "0.0718 "0.000483 κ 3-15*01 1*01 0 "0.0845 "0.00156 Cytoplasmic1081 IgM 3-23*01 1*01 41 0 0.389 "0.00381 κ 1-5*03 2*03 0 0.34 "0.108 Cytoplasmic1124 IgM 1-69*01 6*02 0 0 --NA --NA κ 2D-29*01 4*01 1 "0.303 "0.0174 Cytoplasmic1145 IgG 3-11*03 4*02 22 0 0.401 "0.322 κ 4-1*01 4*01 0 "0.329 "0.0027 Cytoplasmic1152 931 IgG 2-70*01 4*02 34 0 "0.0259 "0.00167 λ 8-61*01 7*01 0 "0.281 "0.00232 Cytoplasmic

3959 κ Cytoplasmic508 IgG 3-21*02 4*02 32 1 0.468 "0.132 λ 3-9*01 3*02 1 "0.0382 "0.00361 Nuclear504 IgG 3-23*01 4*02 49 1 "0.0985 "0.00185 κ 3-11*01 3*01 0 0.174 "0.00702 Nuclear and Cytoplasmic511 IgM 3-15*02 4*02 98 1 "0.186 "0.0185 κ 2-28*01 5*01 0 "0.0834 "0.000155 Nuclear and Cytoplasmic540 IgM 3-23*04 4*02 48 1 "0.296 "0.0123 κ 3-20*01 1*01 0 0.0328 "0.0152 Nuclear and Cytoplasmic575 2044 IgM 4-59*01 6*03 76 2 "0.0716 "0.0000411 κ 1-39*01 1*01 0 "0.027 "0.000000383 Nuclear and Cytoplasmic761 IgG 4-61*01 6*02 24 1 0.196 "0.2 κ 1-17*01 1*01 0 "0.286 0.252 Nuclear and Cytoplasmic1010 IgG 3-15*07 1*01 46 1 "0.0409 "0.0000142 λ 1-40*01 3*02 0 "0.26 "0.00434 Nuclear and Cytoplasmic1102 IgA 4-4*02 4*02 17 0 0.397 "0.00281 λ 3-19*01 2*01 0 "0.353 "0.0411 Nuclear and Cytoplasmic

4343 IgG λ Nuclear and Cytoplasmic4572 κ Nuclear and Cytoplasmic

501 IgM 3-48*03 6*02 31 1 0.362 "0.216 κ 1-5*03 1*01 0 "0.401 "0.00175 Non-Hep-2 Reactive 507 IgA 3-15*01 4*02 17 0 "0.204 "0.00581 κ 4-1*01 3*01 0 0.111 "0.0214 Non-Hep-2 Reactive 513 IgM 1-69*10 6*03 14 1 "0.23 "0.0823 λ 6-57*01 2*01 0 "0.187 "0.00595 Non-Hep-2 Reactive 514 IgM 3-7*01 3*01 52 2 "0.19 "0.0224 λ 5-39*01 3*02 0 "0.105 "0.00371 Non-Hep-2 Reactive 516 IgM 4-59*01 4*02 24 1 "0.204 "0.000583 κ 1-27*01 3*01 0 "0.214 "0.00714 Non-Hep-2 Reactive 520 IgM 4-39*03 4*02 45 1 "0.0671 "0.000116 κ 3-11*01 4*01 0 0.471 "0.0889 Non-Hep-2 Reactive 526 IgM 3-21*02 4*02 27 1 0.375 "0.0105 κ 1-5*03 2*01 0 0.344 "0.0113 Non-Hep-2 Reactive 531 IgG 3-11*01 6*02 48 0 "0.158 "0.000903 λ 7-46*01 2*01 0 "0.35 "0.0254 Non-Hep-2 Reactive 542 IgG 4-34*01 4*02 31 0 "0.405 "0.165 λ 3-25*03 2*01 0 0.49 "0.00129 Non-Hep-2 Reactive 546 IgG 5-51*03 6*02 17 0 "0.062 "0.0739 λ 1-44*01 3*02 0 "0.292 "0.1 Non-Hep-2 Reactive 550 IgG 3-21*01 6*02 36 3 "0.189 "0.00889 κ 3-20*01 1*01 0 0.233 "0.00235 Non-Hep-2 Reactive 565 1918 IgM 3-53*02 6*03 31 1 "0.11 "0.0262 κ 2-28*01 1*01 0 "0.121 "0.0336 Non-Hep-2 Reactive 583 IgM 4-34*01 4*02 19 0 "0.101 "0.0128 κ 1-5*03 1*01 0 0.244 "0.358 Non-Hep-2 Reactive 587 IgM 3-48*01 4*02 19 1 0.479 "0.00531 λ 1-40*01 3*02 1 "0.231 "0.0616 Non-Hep-2 Reactive 598 2056 IgM 4-59*03 4*02 44 1 "0.0903 "0.000389 κ 3-11*01 4*01 3 0.0865 "0.0598 Non-Hep-2 Reactive 600 2357 IgG 6-1*01 4*02 23 1 "0.203 "0.0341 κ 1-5*03 1*01 0 "0.399 "0.000585 Non-Hep-2 Reactive 606 IgG 4-b*01 4*02 27 2 0.0217 "0.209 λ 1-40*01 3*02 0 "0.493 0.446 Non-Hep-2 Reactive 616 1777 IgG 3-48*01 4*02 36 0 "0.00979 "0.00103 λ 1-51*01 1*01 0 "0.288 "0.0412 Non-Hep-2 Reactive 625 IgM 4-61*01 3*02 40 3 "0.344 "0.0429 λ 2-8*01 2*01 1 0.238 "0.293 Non-Hep-2 Reactive 649 IgM 3-23*01 4*02 27 2 0.321 "0.0977 κ 3-11*01 4*01 0 0.434 "0.285 Non-Hep-2 Reactive 657 IgM 3-7*01 4*02 32 1 "0.236 "0.00459 κ 1-39*01 1*01 0 0.0561 "0.208 Non-Hep-2 Reactive 666 1768 IgG 3-11*03 4*02 34 1 "0.0478 "0.00389 κ 3-15*01 2*01 0 0.154 "0.0317 Non-Hep-2 Reactive 670 IgM 4-4*07 5*02 25 2 0.338 "0.212 λ 4-3*01 1*01 0 --NA --NA Non-Hep-2 Reactive 692 1734 IgM 3-23*04 4*02 45 1 "0.475 "0.00654 κ 3-20*01 2*02 0 0.217 "0.0558 Non-Hep-2 Reactive 694 1563 IgM 4-39*01 6*01 37 1 "0.331 "0.000446 κ 3-20*01 3*01 1 0.155 "0.0979 Non-Hep-2 Reactive 697 IgM 3-11*03 4*02 34 2 "0.202 "0.000317 κ 3-20*01 2*01 1 "0.168 "0.0000402 Non-Hep-2 Reactive 702 IgM 3-48*01 4*02 30 1 0.367 "0.11 κ 3-20*01 2*01 0 "0.35 "0.00364 Non-Hep-2 Reactive 706 1850 IgM 3-23*01 3*01 34 1 "0.433 "0.00818 κ 1-6*01 1*01 0 "0.146 "0.0341 Non-Hep-2 Reactive 709 IgM 3-30*09 4*02 26 2 "0.391 "0.0371 κ 1-5*03 1*01 1 "0.401 "0.167 Non-Hep-2 Reactive 721 IgM 3-7*01 4*02 29 1 "0.19 "0.00915 κ 4-1*01 4*01 1 0.105 "0.293 Non-Hep-2 Reactive 727 1793 IgM 4-30-4* 3*02 27 1 0.408 "0.188 λ 1-51*01 1*01 1 "0.31 "0.015 Non-Hep-2 Reactive 733 IgM 3-48*01 4*02 24 1 "0.345 "0.00942 λ 2-14*01 3*02 0 0.199 0.46 Non-Hep-2 Reactive 741 IgG 3-74*01 4*02 22 1 "0.0253 "0.00425 λ 2-8*01 3*02 0 "0.116 "0.0296 Non-Hep-2 Reactive 746 1458 IgM 4-39*02 4*02 28 3 "0.244 "0.0223 λ 2-8*01 2*01 1 0.0202 "0.254 Non-Hep-2 Reactive 748 1537 IgG 3-21*02 3*02 63 1 "0.122 "0.000011 κ 2-30*02 2*01 0 "0.483 "0.423 Non-Hep-2 Reactive 749 3056 IgG 3-15*01 4*02 33 2 "0.273 "0.000844 λ 1-44*01 3*02 1 "0.224 "0.0809 Non-Hep-2 Reactive 750 IgG 3-48*01 5*02 26 2 "0.0545 "0.0000211 λ 2-14*01 3*02 0 0.107 "0.265 Non-Hep-2 Reactive 753 1453 IgG 3-73*01 3*01 56 2 "0.0298 "0.000655 κ 2-28*01 5*01 0 "0.44 "0.112 Non-Hep-2 Reactive 783 IgG 1-18*01 5*02 35 1 "0.126 "0.0306 λ 1-51*02 2*01 0 "0.0738 "0.0171 Non-Hep-2 Reactive 796 IgM 3-23*04 5*01 30 1 0.293 "0.0095 λ 3-25*03 2*01 0 "0.0527 "0.00344 Non-Hep-2 Reactive 825 IgG 4-61*08 1*01 57 0 "0.324 "0.011 κ 4-1*01 5*01 2 0.416 "0.0625 Non-Hep-2 Reactive 884 IgG 3-h*01( 4*02 38 1 "0.382 "0.0386 λ 4-69*02 3*02 0 "0.254 "0.147 Non-Hep-2 Reactive 895 2584 IgM 3-23*04 4*02 32 2 "0.174 "0.0000958 λ 1-51*01 2*01 0 "0.0381 "0.00912 Non-Hep-2 Reactive 901 IgM 3-7*01 6*02 30 1 "0.348 "0.0177 κ 1-9*01 4*01 0 "0.488 "0.00175 Non-Hep-2 Reactive 913 IgM 4-59*01 6*02 14 1 "0.405 "0.0676 κ 4-1*01 2*04 0 0.349 0.417 Non-Hep-2 Reactive 920 IgM 3-23*01 4*02 42 0 0.358 "0.00339 κ 1-39*01 4*01 1 0.196 "0.00504 Non-Hep-2 Reactive 936 IgG 3-11*01 2*01 24 2 "0.211 "0.00709 κ 3-20*01 2*01 1 0.19 "0.000323 Non-Hep-2 Reactive 949 IgG 3-11*03 5*02 41 1 "0.249 "0.0501 λ 1-40*01 2*01 0 "0.0614 "0.000524 Non-Hep-2 Reactive 955 1752 IgM 4-34*01 4*02 22 1 "0.0763 "0.0000943 κ 2-28*01 3*01 0 0.129 "0.261 Non-Hep-2 Reactive 959 1167 IgG 2-5*01 4*02 15 0 0.0342 "0.0642 λ 1-44*01 2*01 1 "0.33 "0.00952 Non-Hep-2 Reactive 975 IgA 3-48*03 6*02 26 1 "0.266 "0.0119 κ 1-5*03 2*02 0 "0.301 "0.0472 Non-Hep-2 Reactive 978 IgG 4-61*01 4*02 43 2 0.149 "0.0206 λ 4-69*01 3*02 0 "0.0533 "0.0000118 Non-Hep-2 Reactive 1024 IgM 3-21*02 3*02 48 1 "0.00492 "7.91E"08 κ 2-30*01 4*01 0 "0.0577 "0.0114 Non-Hep-2 Reactive 1057 IgM 3-23*01 4*02 36 2 0.089 "0.194 λ 3-10*01 3*02 0 0.0628 "0.326 Non-Hep-2 Reactive 1062 1079 IgM 3-23*01 4*02 12 0 "0.0477 "0.00281 κ 1-39*01 2*02 0 0.0807 "0.433 Non-Hep-2 Reactive 1098 1343 IgG 4-39*02 4*02 64 0 "0.284 "0.0201 κ 2-30*01 1*01 1 "0.0297 "0.0114 Non-Hep-2 Reactive 1110 IgM 3-11*01 4*02 36 2 "0.234 "0.0122 κ 2-28*01 2*01 0 "0.225 "0.0000133 Non-Hep-2 Reactive 1116 913 IgM 3-23*01 4*02 35 1 "0.118 "0.00107 κ 1-39*01 5*01 1 0.0558 "0.208 Non-Hep-2 Reactive 1125 IgM 3-7*01 5*01 30 1 "0.145 "0.0573 κ 3-20*01 4*01 0 "0.332 "0.00841 Non-Hep-2 Reactive 1144 IgM 4-39*01 5*02 34 1 0.437 "0.0765 κ 6-21*01 4*01 0 "0.447 "0.00156 Non-Hep-2 Reactive 1149 IgM 3-48*03 4*02 80 1 0.421 "0.0119 κ 3-20*01 5*01 0 "0.123 "0.00204 Non-Hep-2 Reactive 1169 IgM 3-7*01 5*02 47 2 "0.344 "0.000165 κ 3-20*01 1*01 2 "0.0429 "0.0000187 Non-Hep-2 Reactive

4216 IgG κ Non-Hep-2 Reactive 4680 IgG κ Non-Hep-2 Reactive 3519 IgG κ Non-Hep-2 Reactive 3608 IgM λ Non-Hep-2 Reactive 3824 IgG λ Non-Hep-2 Reactive 4366 IgG λ Non-Hep-2 Reactive 4116 IgG λ Non-Hep-2 Reactive 8532 IgM κ Non-Hep-2 Reactive

Table S1. Repertoire and reactivities of tumor immunoglobulins

Patient ID

Stanford Specific

ID

Heavy Chain Light Chain

19389 IgG κ Non-Hep-2 Reactive 4022 IgM κ Non-Hep-2 Reactive

502 IgM 3-23*01 5*02 25 1 0.203 "0.00672 λ 8-61*01 3*02 0 0.164 "0.00984506 IgG 3-33*01 6*02 32 1 "0.0459 "0.000223 λ 1-44*01 1*01 0 "0.128 "0.0541510 IgG 1-3*01 4*02 41 2 "0.231 "0.00415 λ 2-8*01 2*01 0 0.451 "0.108515 IgM 3-74*01 4*02 22 0 "0.108 "0.0301 κ 4-1*01 1*01 1 0.25 "0.224518 IgG 4-59*01 4*03 39 1 "0.135 "0.0133 κ 4-1*01 2*01 1 0.415 "0.249521 IgM 3-53*01 6*02 17 1 "0.374 "0.0122 λ 1-51*01 3*02 0 0.0252 0.254522 IgM 3-23*01 4*02 31 1 0.36 "0.0706 λ 1-40*01 1*01 0 "0.203 "0.00285523 IgM 3-23*01 4*02 30 1 "0.23 "0.0603 λ 3-10*01 2*01 0 "0.427 "0.0591524 IgG 1-18*01 4*02 31 1 0.33 "0.316 λ 1-51*01 2*01 0 "0.485 "0.439529 IgM 3-48*03 3*01 41 1 "0.221 "0.00612 κ 4-1*01 1*01 0 "0.245 "0.0361530 IgG 1-18*01 4*02 30 1 0.33 "0.264 λ 3-19*01 2*01 0 NA NA534 IgG 3-11*03 4*02 31 1 "0.468 "0.00334 λ 1-47*01 2*01 0 "0.214 "0.00000259535 IgM 1-8*01 4*02 40 2 "0.212 "0.0526 κ 4-1*01 1*01 0 "0.219 "0.316536 IgM 3-23*01 5*02 25 1 0.247 "0.116 κ 1-5*03 2*01 2 0.483 "0.000188544 IgM 3-23*05 4*01 33 1 0.332 "0.0956 λ 1-40*01 2*01 0 "0.237 "0.465547 IgM 3-23*01 4*02 42 1 "0.0104 "0.0000113 λ 4-69*02 7*01 0 "0.479 "0.000904548 IgG 3-48*03 2*01 35 1 "0.24 "0.0315 κ 1-5*03 1*01 0 "0.45 "0.067549 IgG 3-48*02 6*02 16 1 "0.105 "0.0121 λ 1-40*01 1*01 0 0.256 "0.149551 IgM 3-23*01 4*02 28 1 "0.272 "0.000108 λ 1-40*01 3*02 0 0.201 "0.344552 IgG 1-18*01 5*02 37 1 "0.391 "0.043 λ 2-8*01 3*02 0 --NA 0.184556 IgM 4-61*02 5*02 34 0 "0.0329 "0.000176 κ 3-20*01 1*01 0 0.252 "0.00516558 3159 IgG 4-34*02 3*01 34 1 "0.401 "0.25 λ 1-40*01 7*01 0 "0.23 "0.0367559 IgG 3-15*01 3*01 30 1 "0.0709 "0.0000137 λ 2-23*01 1*01 0 0.125 "0.0351560 IgM 3-11*01 5*02 32 1 "0.219 "0.0000478 κ 1-39*01 4*01 0 "0.385 "0.00201561 IgG 3-21*02 4*02 47 1 "0.335 "0.000152 λ 1-40*01 3*02 0 0.39 "0.0000693562 IgM 3-21*02 3*02 52 2 "0.208 "0.00163 λ 3-1*01 2*01 0 0.21 "0.127564 IgG 4-59*03 4*02 59 1 "0.143 "0.0278 λ 1-44*01 2*01 0 "0.343 "0.0226566 IgM 3-73*02 4*02 26 1 "0.23 "0.0148 κ 4-1*01 2*01 3 "0.431 "0.316567 IgG 3-23*01 3*02 52 1 "0.357 "0.164 λ 1-51*02 3*02 0 "0.255 "0.0401569 IgG 3-53*01 6*02 44 1 "0.156 "0.0000563 κ 1-5*03 1*01 1 "0.23 "0.0682571 IgM 3-23*05 4*02 32 2 0.444 "0.00058 κ 3-11*01 4*01 2 0.0153 "0.211574 IgG 4-39*03 2*01 24 1 "0.0697 "0.00238 λ 1-40*01 1*01 0 "0.492 "0.33576 IgG 3-23*01 5*02 43 1 "0.182 "0.00433 λ 1-51*01 7*01 0 "0.132 "0.0416578 IgM 4-39*02 3*02 23 4 "0.0493 "0.000145 λ 3-19*01 2*01 0 "0.0249 "0.0000125585 IgM 4-59*07 3*02 28 0 "0.00797 "0.00025 κ 4-1*01 4*01 0 "0.031 "0.00257588 IgG 4-39*02 4*02 57 2 0.00448 0.321 κ 3-20*01 1*01 0 0.197 "0.183589 IgG 3-48*03 5*02 26 0 0.469 "0.0686 κ 6-21*01 1*01 1 0.35 "0.0182591 IgM 3-23*01 4*02 21 1 0.44 "0.0143 κ 4-1*01 4*01 0 "0.431 "0.168592 IgM 1-18*01 4*02 32 2 "0.386 "0.0826 κ 6-21*01 2*01 0 0.35 "0.191594 IgM 4-59*01 6*02 26 3 0.442 "0.0115 λ 9-49*01 3*02 0 "0.0312 "0.000186595 IgM 3-30*04 4*02 23 0 "0.35 "0.0164 λ 1-51*02 1*01 0 "0.0754 0.495597 IgM 3-48*01 4*02 38 1 "0.00979 "0.0018 κ 3-11*01 4*01 0 0.167 "0.00495599 IgG 3-30*04 5*01 39 1 0.35 "0.065 λ 4-69*02 3*02 0 "0.126 "0.00023601 IgM 4-59*02 4*02 47 3 "0.224 "0.108 κ 4-1*01 1*01 0 0.219 "0.0565602 IgG 3-48*03 4*02 24 1 "0.296 "0.0686 λ 1-51*01 2*01 0 0.395 0.486603 IgM 3-30*03 6*02 34 1 "0.169 "0.000969 λ 1-47*01 7*01 0 "0.214 "0.0165604 IgM 4-34*01 3*02 32 2 "0.209 "0.00026 λ 2-8*01 2*01 0 "0.227 "0.431605 IgG 1-18*01 6*02 22 0 "0.0936 "0.0166 λ 1-51*01 3*02 0 0.256 "0.321608 IgG 3-48*02 4*02 43 1 0.213 "0.0551 κ 1-5*03 4*01 0 "0.333 "0.00059609 IgM 3-48*02 4*02 25 2 0.401 "0.0015 κ 3-11*01 4*01 0 "0.253 "0.00598610 IgA 3-23*04 4*02 33 1 "0.107 "0.0101 κ 4-1*01 1*01 0 "0.326 "0.313614 IgM 4-39*02 4*02 36 3 "0.0509 "0.00874 λ 1-51*02 7*01 0 0.212 "0.0765617 IgM 3-23*01 3*01 42 1 "0.122 "0.000222 λ 1-44*01 1*01 0 "0.159 "0.0104620 IgG 4-34*01 3*02 36 2 0.334 "0.206 κ 4-1*01 1*01 0 "0.218 "0.239621 IgG 3-23*04 4*02 53 1 "0.114 "0.000219 λ 8-61*01 3*02 0 "0.104 "0.000103624 IgM 3-48*03 3*02 54 1 "0.0933 "0.00182 κ 1-17*02 1*01 0 "0.194 "0.00281626 IgG 5-51*01 3*02 17 1 "0.15 "0.0741 κ 4-1*01 1*01 1 "0.319 "0.000283627 IgM 3-23*01 6*02 29 1 0.263 "0.0201 κ 6-21*01 1*01 0 --NA 0.189628 IgM 3-7*01 6*03 35 1 "0.0723 "0.000496 κ 4-1*01 2*04 1 0.397 "0.000929629 IgM 3-72*01 6*03 33 1 "0.294 "0.0000443 λ 1-51*01 3*02 0 "0.323 "0.0287630 IgM 3-30*03 4*02 34 0 "0.0326 "0.0051 κ 4-1*01 4*01 0 "0.0111 "0.000926631 IgM 4-34*02 3*01 44 1 "0.216 "0.00988 κ 4-1*01 1*01 0 "0.262 "0.000324635 IgM 4-59*01 4*02 51 0 "0.243 "0.000651 λ 4-69*02 3*02 1 "0.0238 "0.000835636 IgM 4-59*02 6*02 31 2 "0.32 "0.000255 κ 1-9*01 2*01 0 "0.373 "0.149638 IgM 3-48*01 5*02 33 1 "0.14 "0.000747 λ 1-44*01 2*01 0 0.0775 0.438640 IgM 3-23*01 6*02 39 1 "0.357 "0.00339 κ 3-20*01 4*01 0 0.127 0.462641 IgG 3-23*04 5*02 38 1 0.475 "0.0274 κ 1-12*01 3*01 0 0.218 "0.114642 IgG 3-23*01 4*02 58 2 0.0288 "0.116 λ 2-14*01 2*01 1 0.459 "0.00263645 IgM 3-74*01 6*02 25 0 "0.251 "0.154 λ 2-14*01 2*01 1 0.319 "0.0658647 IgM 3-48*03 4*02 16 1 0.34 "0.447 λ 2-14*01 2*01 0 0.00417 0.237648 IgG 4-34*01 4*02 70 1 "0.118 "0.0126 λ 7-43*01 3*02 0 "0.216 "0.0419650 IgM 3-66*02 3*02 57 4 "0.283 "0.0102 λ 3-19*01 3*02 0 "0.479 "0.00124652 IgG 3-15*01 4*02 33 1 0.392 "0.366 λ 1-40*01 2*01 0 "0.324 "0.147654 IgG 3-23*01 4*02 30 0 0.198 0.192 κ 6-21*01 5*01 0 "0.166 "0.0117655 IgG 3-7*01 6*02 29 1 "0.145 "0.0404 κ 3-20*01 3*01 0 0.409 "0.00619659 IgG 3-21*01 6*02 20 3 "0.236 "0.00459 κ 4-1*01 1*01 0 0.369 "0.0565660 IgM 3-23*01 4*02 46 2 0.0974 "0.0818 κ 3-20*01 1*01 1 0.488 "0.00512662 IgG 3-48*01 4*02 14 0 "0.0321 "0.000756 λ 2-8*01 2*01 0 "0.323 "0.0616663 IgM 3-66*01 3*02 29 2 "0.439 "0.00559 λ 7-43*01 3*02 0 "0.463 "0.207665 IgG 3-33*01 4*02 20 1 "0.188 "0.0562 λ 1-51*01 2*01 1 "0.31 "0.015667 IgM 3-23*01 4*02 25 1 0.198 0.499 κ 1-5*03 1*01 0 "0.361 "0.213668 IgG 3-23*01 3*01 40 1 0.116 "0.268 κ 3-20*01 5*01 0 0.358 "0.11669 IgM 3-30*03 4*02 31 0 0.331 "0.0312 κ 3-15*01 1*01 0 "0.0988 "0.0103671 IgM 4-39*01 6*02 23 1 0.333 "0.0616 κ 3-15*01 2*02 0 0.209 "0.0465673 IgM 4-59*08 6*02 48 3 "0.263 "0.00073 κ 3-20*01 2*02 0 "0.153 "0.00000486675 IgG 3-15*01 6*03 40 1 "0.0218 "0.001 κ 2-28*01 3*01 1 "0.176 "0.00077678 IgG 4-59*01 4*02 37 0 "0.0858 "0.000474 λ 1-44*01 2*01 0 "0.0946 "0.00386684 IgM 3-7*01 4*02 33 1 0.312 "0.377 κ 1-27*01 1*01 0 0.241 "0.441685 IgM 3-11*01 4*02 37 1 "0.136 "0.000651 κ 3-20*01 1*01 0 0.229 "0.00649687 IgM 3-7*01 6*02 32 1 "0.119 "0.0012 κ 1-9*01 4*01 0 0.0269 "0.313688 IgM 3-23*04 5*02 54 1 "0.467 "0.000148 λ 1-44*01 7*01 0 0.369 "0.0113690 IgG 3-30*04 4*02 48 1 "0.261 "0.0392 λ 1-51*01 7*01 1 0.459 "0.0125698 IgM 4-59*01 6*01 38 3 "0.45 "0.00211 λ 3-19*01 2*01 0 0.252 "0.0339700 IgG 1-46*01 5*02 33 1 0.218 "0.025 λ 1-40*01 3*02 0 "0.231 "0.0616701 1640 IgM 3-7*01 4*02 34 0 "0.0863 "0.019 κ 1-5*03 1*01 0 0.328 0.393704 IgM 3-23*01 4*02 49 0 "0.123 "0.000602 κ 3-15*01 1*01 1 0.143 "0.254705 IgG 3-7*01 4*02 38 1 "0.444 "0.019 κ 3-20*01 1*01 0 "0.393 "0.139710 IgM 1-8*01 4*02 35 1 "0.245 "0.000071 λ 2-8*01 2*01 0 0.0255 "0.403711 IgG 3-30*04 4*02 30 1 "0.279 "0.179 λ 4-69*01 2*01 1 "0.0389 "0.00736

712 IgM 3-23*01 4*02 51 1 0.415 "0.0451 κ 4-1*01 1*01 0 0.25 "0.00126713 IgM 3-7*01 4*02 33 2 "0.112 "0.0336 κ 2-28*01 4*01 3 "0.00405 0.336714 IgG 3-11*03 4*02 26 1 "0.0311 "0.00334 κ 2-30*01 1*01 0 "0.337 "0.316716 IgM 3-23*04 4*02 30 1 "0.213 "0.000093 κ 2-30*02 4*01 0 "0.0274 "0.0283720 IgM 3-48*03 4*02 44 1 0.3 "0.134 κ 3-20*01 1*01 0 "0.401 "0.0234722 IgA 4-39*07 2*01 37 3 "0.0412 "0.0000259 λ 1-51*01 7*01 0 0.296 "0.169723 IgM 4-39*03 4*02 44 1 "0.0686 "0.000926 λ 2-8*01 2*01 0 0.353 "0.289725 IgG 4-59*03 5*01 65 2 "0.051 "0.000185 λ 1-44*01 1*01 0 "0.219 "0.0245728 IgM 3-48*02 4*02 21 1 0.193 "0.00882 κ 3-11*01 2*04 0 0.0125 "0.157730 IgG 3-23*01 3*02 42 1 "0.212 "0.00000448 λ 1-44*01 7*01 0 0.126 "0.000968731 IgM 3-30*03 4*02 15 3 0.181 0.458 λ 10-54*0 3*02 1 "0.308 0.469734 IgM 4-34*01 3*02 56 0 "0.0483 "0.0000394 κ 4-1*01 2*01 1 "0.451 "0.00182735 IgM 3-21*02 4*02 28 1 "0.152 "0.000988 κ 1-17*01 2*01 0 0.114 "0.295737 IgA 3-11*01 3*01 29 1 "0.312 "0.0483 κ 3-20*01 2*01 0 0.206 "0.0815738 IgM 5-51*01 4*02 8 0 "0.42 "0.421 λ 3-19*01 2*01 0 0.252 "0.0111742 IgG 3-11*03 6*02 36 2 "0.27 "0.071 κ 1-17*01 1*01 0 "0.378 "0.000712745 IgM 3-23*01 4*02 33 1 "0.0226 "0.0102 κ 3-20*01 2*02 0 0.26 "0.0139747 IgG 3-23*01 4*02 44 0 0.0706 "0.123 λ 2-14*01 3*02 0 0.00609 "0.059752 IgG 3-7*01 5*02 39 1 0.473 "0.00336 λ 1-44*01 7*01 0 "0.0044 "0.0015754 IgM 3-48*03 6*02 37 1 0.413 "0.000106 λ 4-69*01 3*02 1 "0.374 "0.00579758 IgM 3-48*03 4*02 26 1 0.293 "0.309 κ 4-1*01 1*01 0 "0.245 "0.098760 IgM 4-34*02 4*02 37 1 "0.0833 "0.00047 κ 2-30*02 1*01 0 0.115 "0.188762 IgA 3-7*01 4*02 32 1 0.348 "0.000656 λ 3-10*01 3*02 0 0.106 "0.431763 IgM 4-59*01 2*01 26 0 "0.204 "0.00272 κ 4-1*01 1*01 0 "0.154 "0.00796764 IgM 3-48*03 4*02 40 0 "0.4 "0.195 κ 2-28*01 1*01 0 "0.23 "0.0607766 IgG 3-23*01 6*02 57 1 0.305 "0.029 κ 1-5*03 3*01 0 "0.259 "0.00277767 IgM 4-34*01 5*02 36 0 "0.305 "0.00105 λ 1-51*01 3*02 1 "0.0981 "0.00551768 IgM 3-15*01 6*03 28 1 "0.221 "0.00733 λ 1-40*01 3*02 0 "0.138 "0.0235769 IgG 3-23*01 4*02 34 1 "0.265 "0.000267 λ 1-44*01 7*01 0 "0.36 "0.0188770 IgM 3-48*03 5*02 11 1 0.219 0.346 κ 1-12*01 4*01 0 "0.197 "0.0484771 IgM 3-74*01 5*02 25 1 "0.466 "0.00225 κ 1-39*01 3*01 0 0.248 "0.0616772 IgM 3-66*01 4*02 43 3 "0.417 "0.111 κ 1-5*03 2*02 1 0.165 "0.0674773 IgG 3-30*03 6*02 44 1 0.357 0.439 λ 1-47*01 1*01 0 "0.458 "0.00542775 IgM 3-7*01 6*02 26 3 "0.0863 "0.000293 λ 3-21*02 3*02 1 0.388 "0.0688776 IgG 3-11*01 3*01 48 2 0.324 "0.011 λ 1-51*01 2*01 0 0.469 "0.00467778 IgM 3-23*04 4*02 28 1 "0.221 "0.00254 κ 3-11*01 4*01 0 0.471 "0.00497779 IgM 3-21*02 6*02 24 1 "0.432 "0.00533 λ 2-14*01 2*01 0 0.00288 0.27780 IgG 3-15*01 5*01 44 1 "0.0489 "0.000254 κ 1-17*01 2*01 1 "0.271 "0.0000721781 IgM 3-7*01 4*02 38 1 0.0198 "0.272 κ 3-20*01 4*01 0 0.119 "0.108782 IgG 6-1*01, 1*01 90 1 "0.225 "0.0421 κ 3-20*01 3*01 0 0.357 "0.00191786 IgM 3-7*01 4*02 22 0 0.192 "0.311 κ 3-15*01 4*01 0 "0.338 "0.0802787 IgM 3-23*01 5*02 36 3 0.0123 "0.226 λ 2-11*01 2*01 0 "0.142 0.5788 IgM 3-30*03 4*02 18 1 "0.438 "0.441 λ 2-23*02 3*02 0 0.0109 "0.264789 IgM 4-39*02 3*01 47 1 "0.151 "0.00194 κ 1-5*03 1*01 0 0.233 "0.0000607790 IgM 1-3*01 4*02 24 1 "0.233 "0.0555 λ 4-69*01 7*01 0 0.0632 "0.00686791 IgM 3-30*03 3*02 46 1 "0.211 "0.146 κ 4-1*01 4*01 0 "0.0299 "0.0000126792 IgG 1-3*01 5*02 31 1 "0.258 "0.0179 κ 4-1*01 4*01 0 "0.313 "0.139793 IgG 3-30*09 6*02 55 1 "0.00659 "0.0134 λ 1-47*01 2*01 0 "0.48 "0.000173794 IgM 1-18*01 6*02 31 2 0.209 "0.041 κ 3-20*01 3*01 1 0.362 "0.00386797 IgG 4-61*02 6*03 33 3 "0.126 "0.0000116 κ 2-28*01 4*01 1 "0.169 "0.237798 IgM 3-7*01 3*01 27 1 "0.19 "0.00293 κ 2-24*01 2*01 0 "0.182 "0.0452799 IgM 3-53*01 5*01 36 1 0.162 "0.0469 κ 4-1*01 1*01 1 "0.313 "0.0696800 IgM 3-66*02 6*02 14 1 "0.0965 "0.135 λ 4-69*01 3*02 0 "0.137 "0.0224801 IgM 3-48*03 6*02 27 1 "0.0371 "0.0257 κ 3-11*01 4*01 0 0.00487 "0.157803 IgG 3-23*01 4*02 31 1 0.164 "0.383 λ 4-69*01 1*01 1 "0.417 "0.19805 IgM 3-7*01 4*02 28 1 "0.151 "0.00135 κ 3-11*01 4*01 0 0.471 "0.0377806 IgM 3-48*01 5*01 45 1 0.274 "0.219 λ 10-54*0 2*01 0 "0.302 "0.0106808 IgM 3-48*03 4*02 24 1 0.205 "0.146 κ 3-11*01 4*01 0 0.371 "0.0453809 IgG 4-30-4* 3*02 45 3 "0.212 "0.000833 κ 1-17*02 2*01 0 0.231 "0.325810 IgM 4-59*03 3*01 31 1 0.399 "0.027 κ 3-20*01 2*02 0 0.203 "0.00204813 IgM 1-2*02 6*02 38 1 "0.115 "0.0000934 λ 3-1*01 3*02 0 0.169 "0.0835816 IgG 3-74*01 4*02 25 1 "0.189 "0.00425 κ 4-1*01 3*01 0 "0.402 "0.00258820 IgM 3-21*01 3*02 28 0 "0.00589 "0.0000318 λ 1-44*01 3*02 0 "0.218 "0.0819821 IgM 4-34*01 3*02 51 0 "0.0215 "0.0824 κ 3-11*01 4*01 0 "0.491 "0.0167822 IgM 3-11*01 4*02 39 1 "0.0911 "0.000807 κ 3-20*01 1*01 0 0.0811 "0.00771823 IgM 3-11*01 6*02 14 2 "0.061 "0.000635 κ 3-15*01 4*01 0 "0.34 "0.0512826 IgG 4-34*02 3*01 41 1 "0.14 "0.0042 λ 3-25*03 3*02 0 "0.25 "0.000403827 IgM 3-53*01 4*02 36 2 "0.443 "0.0228 κ 3-11*01 4*01 1 0.0183 0.463828 IgM 3-23*01 5*02 40 1 "0.374 "0.0211 κ 3-15*01 1*01 0 "0.226 "0.0176829 IgM 4-61*01 3*02 41 0 "0.000207 "0.0000006 κ 1-39*01 1*01 1 "0.0256 "0.00141830 IgM 3-11*01 5*02 47 3 "0.122 "0.00000328 λ 6-57*01 7*01 0 0.181 "0.217831 IgG 3-74*03 4*02 73 1 "0.421 "0.000531 κ 1-12*01 5*01 0 "0.49 "0.016833 IgM 4-39*01 3*02 25 2 "0.463 "0.118 λ 1-40*01 2*01 1 0.282 "0.134834 IgM 3-23*01 5*02 43 1 "0.344 "0.000901 κ 1-5*03 1*01 0 "0.0908 "0.0000633836 IgM 3-48*02 4*02 25 1 "0.246 "0.0291 λ 1-51*01 3*02 0 0.149 "0.0509837 IgG 3-23*01 2*01 68 1 "0.0422 "0.0108 λ 1-51*01 7*01 0 "0.154 "0.00813838 IgM 3-7*01 4*02 23 0 "0.0774 "0.00332 κ 4-1*01 2*01 0 "0.031 "0.00773839 IgG 3-23*04 4*02 53 1 "0.195 "0.000423 κ 4-1*01 3*01 1 "0.49 "0.152841 IgM 4-59*01 2*01 43 4 0.107 "0.449 λ 1-44*01 3*02 1 0.205 "0.297843 IgG 3-11*01 6*02 32 2 "0.168 "0.092 κ 2-28*01 2*01 0 "0.363 "0.063844 IgM 3-7*01 4*02 28 1 "0.123 "0.000000636 κ 4-1*01 2*02 0 0.121 "0.007846 IgM 3-23*01 5*02 40 1 "0.455 "0.00196 λ 2-8*01 2*01 1 0.49 "0.00534848 IgM 3-66*01 4*02 50 1 "0.168 "0.00129 λ 2-11*01 2*01 0 "0.437 "0.0473850 IgM 3-23*01 4*02 25 0 "0.231 "0.0573 κ 2-30*02 2*01 0 "0.13 "0.141851 IgM 4-59*01 6*02 37 2 0.387 "0.00417 κ 3-20*01 1*01 0 "0.425 "0.00132852 IgM 4-61*02 6*02 21 2 0.389 "0.0577 κ 3-20*01 1*01 1 "0.35 "0.00364853 IgM 4-39*01 4*02 36 1 "0.453 "0.00356 λ 2-11*01 2*01 0 0.245 "0.202857 IgA 5-51*01 4*02 32 0 "0.261 "0.0782 λ 2-14*01 2*01 0 0.336 "0.416859 IgM 3-21*02 4*02 26 1 "0.148 "0.00927 λ 1-36*01 3*02 0 "0.0641 "0.00000369860 IgG 1-46*01 3*01 59 1 "0.337 "0.0189 λ 3-25*03 3*02 0 "0.155 "0.000996861 IgG 3-48*02 4*02 24 0 "0.039 "0.00000229 λ 2-14*01 3*02 0 0.498 "0.0105862 IgM 2-26*01 6*02 0 0 --NA --NA κ 1-17*01 4*01 0 "0.219 "0.000136863 IgM 1-46*01 4*02 24 1 "0.484 "0.0112 λ 1-51*01 7*01 0 "0.0741 "0.0628866 IgM 3-7*01 6*02 31 1 0.489 "0.00293 κ 4-1*01 2*02 1 0.425 "0.000814867 IgM 4-39*01 5*02 31 1 0.0243 0.339 λ 2-14*01 3*02 0 0.0765 "0.238868 IgM 3-7*01 3*02 20 1 0.00083 "0.459 κ 1-9*01 4*01 0 0.0269 "0.39869 IgM 3-23*04 4*02 40 2 "0.374 "0.143 λ 4-69*01 3*02 1 0.278 "0.0329870 IgM 4-4*07 4*02 45 2 "0.0269 "0.00000103 κ 3-20*01 5*01 0 "0.262 "0.00208871 IgM 3-21*02 4*02 35 1 0.468 "0.171 κ 4-1*01 3*01 0 0.291 0.25872 IgG 3-23*04 3*02 29 1 "0.326 "0.0248 κ 3-20*01 1*01 0 "0.209 "0.00922873 IgG 3-49*03 4*02 35 0 "0.108 "0.00222 λ 1-40*01 1*01 1 "0.488 "0.118

877 IgG 3-48*01 4*02 35 1 0.0524 "0.11 λ 2-18*02 2*01 0 0.147 0.403878 IgG 4-34*02 4*02 57 0 "0.0502 "0.000000766 κ 2-24*01 1*01 0 "0.133 "0.036879 IgA 5-51*01 4*02 21 0 "0.211 "0.00418 λ 3-25*03 7*01 0 "0.25 "0.00085881 IgM 4-39*07 6*02 36 2 "0.288 "0.129 κ 2-28*01 1*01 1 "0.0349 "0.00233883 IgM 4-59*02 6*02 43 0 "0.0907 "0.000669 κ 3-15*01 2*01 0 0.0878 "0.194888 IgM 4-39*01 1*01 41 1 0.104 0.305 κ 3-20*01 3*01 0 0.396 "0.0325889 IgG 4-b*02 1*01 31 2 "0.0913 "0.00168 κ 4-1*01 1*01 0 "0.489 "0.00269891 IgM 3-11*01 4*02 29 1 "0.423 "0.0126 κ 4-1*01 2*01 1 "0.0781 "0.00000483893 IgG 3-15*04 6*02 32 1 "0.256 "0.00323 κ 1-39*01 4*01 1 "0.162 "0.0188894 IgG 4-31*03 5*02 47 1 0.421 "0.0759 λ 2-11*02 2*01 1 0.0241 "0.349896 IgM 3-74*01 5*02 36 1 0.421 "0.22 κ 4-1*01 2*01 0 "0.0775 "0.0489898 IgM 3-15*01 4*02 20 0 "0.4 "0.00249 κ 4-1*01 1*01 1 0.0315 "0.139899 IgM 4-34*01 3*01 27 2 0.315 "0.177 κ 4-1*01 4*01 0 "0.154 "0.0807902 IgG 3-7*01 5*02 27 1 "0.236 "0.000119 κ 3-20*01 5*01 0 0.0568 "0.071903 IgM 4-59*01 4*03 29 1 0.201 "0.134 κ 3-20*01 4*01 0 0.122 "0.0499904 IgG 4-4*02 4*02 43 2 "0.183 "0.00491 λ 2-23*02 2*01 0 0.173 "0.119905 IgG 3-7*01 5*01 29 1 "0.236 "0.000656 λ 1-40*01 7*01 1 0.429 "0.0616908 IgG 1-46*01 5*01 72 0 "0.279 "0.0000719 λ 2-11*03 3*02 0 "0.268 "0.0453911 IgG 4-59*03 4*02 48 3 "0.35 "0.000000811 λ 1-47*01 7*01 1 0.235 "0.231915 IgG 3-53*01 4*02 27 1 "0.112 "0.00188 λ 4-69*02 3*02 0 "0.0279 "0.00179916 IgM 3-48*01 4*02 20 1 "0.188 "0.000103 κ 1-5*03 1*01 0 0.0195 "0.157918 IgG 3-11*01 6*02 38 1 0.479 "0.0188 κ 3-20*01 4*01 0 0.0397 "0.0182919 IgG 3-23*01 5*01 29 1 "0.224 "0.000045 λ 1-51*02 7*01 1 "0.227 "0.02921 IgM 3-15*01 4*02 33 0 "0.134 "0.0297 κ 3-20*01 5*01 1 "0.213 "0.0189922 IgM 3-15*02 4*02 46 1 "0.1 "0.00044 κ 1-5*03 1*01 0 "0.215 "0.000612923 IgG 3-48*01 4*01 26 0 "0.0298 "0.0000211 κ 3-11*01 4*01 1 "0.401 "0.0796924 IgG 3-23*01 6*02 46 0 "0.0312 "0.00881 λ 10-54*0 3*02 0 "0.191 "0.00187927 IgG 3-30*14 1*01 47 1 "0.0525 "0.0616 κ 3-15*01 4*01 0 0.355 "0.000467929 IgM 3-15*01 4*02 18 0 "0.497 "0.0516 κ 1-39*01 3*01 1 0.466 "0.0242931 IgG 7-4-1*0 4*01 21 0 "0.165 "0.0162 λ 3-19*01 3*02 0 "0.281 "0.00000238932 IgA 2-5*10 5*02 25 0 "0.0925 "0.00748 λ 3-10*01 1*01 0 0.451 "0.00574933 IgM 4-59*01 3*02 41 2 0.173 "0.0305 κ 1-5*03 4*01 0 0.155 "0.0596940 IgM 4-34*02 6*03 30 2 "0.282 "0.0236 κ 1-27*01 1*01 0 0.0112 "0.162941 IgG 3-30*02 4*02 29 0 0.297 "0.342 κ 1-39*01 1*01 1 0.245 "0.0729942 IgG 3-23*01 5*01 42 1 0.182 "0.00723 κ 1-8*01 1*01 0 "0.455 "0.0264943 IgA 3-23*04 5*02 38 1 0.466 "0.000451 λ 2-8*01 1*01 1 0.282 "0.00657945 IgM 4-59*01 6*02 25 3 "0.454 "0.134 κ 1-39*01 4*01 0 0.0797 "0.165947 IgM 3-23*01 4*02 40 2 "0.0732 "0.000901 λ 1-40*01 3*02 0 "0.178 "0.00373948 IgM 3-53*02 4*02 20 2 "0.135 "0.00477 λ 2-8*01 3*02 0 0.427 "0.126952 IgM 3-66*01 4*02 24 1 "0.0941 "0.0116 κ 4-1*01 1*01 0 "0.0441 "0.127957 IgG 3-21*01 4*02 26 0 0.471 "0.0584 κ 4-1*01 4*01 0 0.368 "0.24958 IgM 3-11*01 6*03 20 1 "0.0227 "0.0712 λ 3-19*01 2*01 0 0.131 "0.233960 IgM 3-23*04 4*02 35 1 0.147 "0.232 κ 2-30*02 1*01 0 0.429 "0.08962 IgM 3-53*02 2*01 25 1 "0.461 "0.000882 κ 4-1*01 1*01 1 0.218 "0.393965 IgM 3-23*01 6*02 3 1 "0.134 "0.111 κ 1-16*02 4*01 0 0.248 0.335966 IgM 4-39*01 4*02 28 2 "0.155 "0.000936 λ 4-69*01 7*01 0 "0.0121 "0.00000488967 IgM 3-23*01 4*02 38 1 "0.357 "0.00196 κ 1-9*01 4*01 0 "0.438 "0.0295968 IgM 3-23*04 6*02 26 1 "0.138 "0.000504 λ 1-51*01 3*02 0 "0.131 "0.0412969 IgG 3-48*03 6*03 46 1 "0.147 "0.000959 κ 1-12*01 1*01 0 "0.227 "0.000451973 IgG 1-2*02 3*01 24 0 "0.135 "0.0143 κ 3-20*01 1*01 0 "0.401 "0.0234976 IgG 3-11*01 3*01 26 1 0.168 "0.201 λ 7-43*01 3*02 1 0.0905 0.122979 IgG 4-34*01 6*03 70 2 "0.0879 "0.00678 κ 2-24*01 2*01 0 "0.245 "0.000397980 IgG 3-48*02 3*01 60 0 "0.221 "0.000805 κ 3-15*01 1*01 0 "0.0988 "0.000425981 IgM 3-74*02 4*02 26 1 "0.108 "0.128 κ 4-1*01 4*01 0 --NA --NA982 IgM 3-53*02 4*02 29 0 0.0365 "0.134 κ 1-27*01 3*01 1 "0.131 "0.00838983 IgG 3-7*01 1*01 35 0 "0.465 "0.0404 κ 1-39*01 4*01 1 0.16 "0.0321984 IgM 3-7*01 4*02 27 0 "0.288 "0.0007 κ 1-17*01 1*01 0 0.465 "0.0000202985 IgM 3-11*01 5*02 34 2 "0.492 "0.0146 λ 1-40*01 2*01 2 "0.256 "0.428986 IgA 3-48*01 4*02 31 0 "0.14 "0.000152 κ 2-28*01 2*02 0 "0.025 "0.000701987 IgG 3-23*01 4*02 43 0 "0.436 "0.000901 κ 4-1*01 3*01 0 "0.183 "0.000813989 IgG 3-23*01 4*02 29 1 "0.0688 "0.0000494 λ 1-44*01 7*01 0 "0.0946 "0.124990 IgM 3-48*02 5*02 42 4 "0.433 "0.00807 κ 1-39*01 1*01 1 0.182 "0.175991 IgG 3-23*01 4*02 26 1 0.357 "0.264 κ 1-39*01 2*03 0 "0.34 "0.0262992 IgG 3-11*03 4*02 25 1 "0.165 "0.278 κ 3-20*01 3*01 0 0.00051 "0.265993 IgM 3-30*03 4*02 39 0 "0.297 "0.0148 κ 3-11*01 3*01 0 "0.221 "0.00344994 IgG 3-48*03 4*02 35 2 "0.124 "0.0261 λ 2-11*01 2*01 0 0.0497 "0.0381996 IgM 4-31*06 5*02 29 0 "0.142 "0.0053 λ 1-51*01 1*01 0 "0.129 "0.00119997 IgM 3-74*01 5*02 24 1 "0.0844 "0.000938 λ 1-47*01 7*01 2 0.436 "0.343999 IgG 3-21*02 5*02 37 3 0.289 "0.0343 λ 3-10*01 3*02 1 0.243 "0.002871004 IgG 3-53*01 3*02 42 1 0.231 "0.184 λ 2-11*01 3*02 0 0.245 "0.3761005 IgM 4-34*01 5*02 54 0 "0.196 "1.29E"08 λ 1-40*01 3*02 0 "0.419 "0.001081006 IgM 4-59*01 4*02 33 0 "0.0375 "0.00000937 κ 1-33*01 2*01 0 "0.431 "0.0002241007 IgM 3-7*01 4*02 35 1 "0.465 "0.0782 κ 1-9*01 3*01 0 "0.438 "0.02951008 IgG 3-74*01 5*01 41 1 0.218 "0.152 λ 1-51*01 3*02 0 0.319 "0.008031009 IgM 3-23*01 4*02 26 1 "0.23 "0.0104 κ 3-20*01 1*01 0 0.0725 "0.03071012 IgM 3-48*02 4*02 24 1 "0.138 "0.00000608 κ 1-27*01 4*01 0 "0.433 "0.007251014 IgM 3-53*01 4*02 28 1 "0.122 "0.00000328 κ 4-1*01 3*01 0 "0.0636 "0.2391016 IgM 3-48*01 6*02 36 1 "0.049 "0.00117 κ 3-15*01 1*01 0 0.078 "0.01791017 IgM 3-48*01 6*02 50 1 "0.322 "0.0000232 κ 3-20*01 1*01 0 0.219 "0.1511022 IgG 1-18*01 4*02 17 0 0.304 "0.482 λ 2-23*01 2*01 0 0.205 "0.002491023 IgM 4-4*07 5*01 51 1 "0.385 "0.0000119 κ 4-1*01 1*01 0 "0.391 "0.008331025 IgM 3-30*01 3*02 20 1 0.246 "0.187 κ 1-5*03 1*01 0 0.0541 "0.01931028 IgG 3-48*03 4*02 39 1 "0.246 "0.00307 κ 3-11*01 2*01 0 0.0949 "0.08941030 IgG 4-39*07 4*02 48 1 "0.158 "0.00394 λ 2-8*01 3*02 0 0.00249 "0.2061031 IgG 3-21*02 6*01 52 2 "0.208 "0.00635 κ 3-11*01 3*01 3 0.086 "0.03771032 IgG 3-23*01 3*01 39 1 "0.388 "0.192 κ 3-11*01 2*01 0 0.129 "0.01981033 IgG 3-23*01 3*02 27 0 0.321 "0.0672 κ 1-27*01 4*01 1 0.185 "0.3071034 IgM 3-11*03 4*01 31 2 "0.367 "0.00605 κ 3-11*01 5*01 0 "0.49 "0.03631035 IgM 3-49*04 4*02 44 1 "0.122 "0.00228 κ 2-28*01 3*01 0 "0.352 "0.02011036 IgM 3-48*01 4*02 45 2 "0.12 "0.000448 κ 3-11*01 5*01 0 "0.261 "0.2511039 IgG 4-59*01 6*02 33 2 0.296 "0.0173 λ 3-21*01 3*02 0 0.419 "0.06311040 IgM 3-30*03 4*02 31 0 "0.0802 "0.0316 κ 3-20*01 3*01 1 0.304 "0.0005481041 IgG 3-7*01 4*02 26 1 "0.288 "0.0007 κ 1-5*03 1*01 0 0.0259 0.3921042 IgM 3-53*01 6*02 30 1 0.178 "0.0151 λ 2-8*01 2*01 0 "0.227 "0.4311043 IgM 3-48*02 4*02 39 2 "0.0229 "0.00000194 κ 2-30*01 2*01 0 "0.21 "0.04891044 IgM 3-23*04 4*02 55 2 0.49 "0.0583 κ 3-11*01 4*01 1 0.386 "0.1181045 IgG 3-11*03 6*01 22 2 "0.211 "0.0519 λ 2-14*01 3*02 0 0.0101 "0.2721047 IgM 3-21*01 4*02 44 1 0.216 "0.196 λ 1-51*01 3*02 1 0.185 "0.05651049 IgG 4-59*03 4*02 38 2 0.162 "0.249 λ 1-44*01 3*02 0 "0.278 "0.00151050 IgG 4-39*01 6*02 53 1 "0.364 "0.00806 κ 1-17*01 1*01 0 "0.379 "0.01931052 IgM 3-7*01 4*02 24 1 0.387 "0.00824 κ 3D-20*0 3*01 1 0.445 "0.00601

1053 IgM 1-2*04 6*02 6 0 "0.0992 "0.149 λ 2-11*01 1*01 1 0.304 "0.1051054 IgG 4-31*03 4*02 23 0 0.185 "0.0138 κ 2-28*01 1*01 0 0.228 "0.2181056 IgG 3-h*02( 6*02 42 0 "0.132 "0.0476 λ 2-14*01 2*01 0 "0.168 "0.00002381058 IgM 3-7*01 4*02 31 2 0.489 "0.00535 κ 4-1*01 2*01 0 0.0657 "0.3161060 IgM 4-39*01 4*02 48 4 "0.217 "0.00127 λ 2-23*01 2*01 0 0.0531 "0.2781061 IgG 3-53*01 6*02 44 1 0.378 "0.00695 κ 4-1*01 1*01 0 "0.0746 "0.02391065 IgM 4-34*02 4*02 30 2 0.405 "0.0273 λ 1-51*02 3*02 2 "0.185 "0.09611066 IgM 3-48*03 3*02 21 2 "0.19 "0.0287 κ 1-39*01 2*01 2 "0.43 "0.01381067 IgG 1-8*01 6*02 28 1 "0.169 "0.0402 λ 2-23*01 2*01 0 0.0477 0.4441068 IgG 3-48*01 5*02 28 0 "0.104 "0.00179 κ 1-5*03 1*01 1 "0.45 "0.0671075 IgM 3-48*01 5*02 34 0 "0.0355 "7.71E"08 κ 1-39*01 5*01 1 0.0558 "0.2961076 IgG 3-66*01 3*02 64 1 "0.159 "0.000471 λ 4-69*01 3*02 0 0.224 "0.007181077 IgM 4-4*07 6*02 47 3 "0.115 "0.00000103 κ 3-20*01 4*01 0 "0.351 "0.01821078 IgM 1-8*01 4*03 46 2 "0.166 "0.00287 λ 3-10*01 2*01 0 "0.283 "0.008321080 IgM 4-39*01 4*02 36 3 0.437 "0.137 κ 4-1*01 4*01 0 "0.431 "0.0071082 IgM 4-34*01 4*02 35 2 "0.115 "0.0262 λ 10-54*0 3*02 0 "0.0935 "0.2891083 IgG 3-23*05 6*02 25 2 0.332 "0.37 λ 1-44*01 7*01 1 "0.137 "0.01111084 IgG 3-30*03 6*03 42 1 "0.297 "0.031 λ 4-69*01 2*01 0 "0.266 "0.000381086 IgM 3-15*04 4*02 26 0 "0.192 "0.0143 κ 1-5*03 1*01 0 "0.101 "0.001751087 IgM 3-23*01 6*02 12 1 0.0113 0.142 κ 1-39*01 1*01 0 0.314 "0.06471088 IgG 2-5*10 4*02 8 0 "0.317 "0.241 κ 4-1*01 1*01 0 "0.262 "0.008331089 IgM 3-73*02 5*02 30 1 0.471 "0.375 λ 2-23*01 7*01 1 0.0206 "0.2011091 IgM 4-4*02 5*01 31 1 "0.363 "0.129 λ 1-40*01 2*01 0 "0.0233 "0.00006931092 IgM 1-18*01 4*02 23 1 "0.466 "0.0205 κ 1-5*03 3*01 0 0.173 "0.0001741093 IgM 3-48*02 6*02 35 1 0.498 "0.000964 κ 1-9*01 4*01 0 "0.449 "0.09651095 IgM 4-34*02 6*03 34 0 "0.0833 "0.000052 κ 3-20*01 1*01 0 0.42 "0.002051096 IgG 3-30*03 5*02 39 0 0.155 "0.0214 λ 1-51*01 3*02 0 0.127 "0.07111097 IgG 3-30*03 4*02 24 1 0.228 "0.196 κ 2-24*01 2*01 0 "0.235 "0.4461099 IgM 3-74*02 6*02 15 1 "0.139 "0.0275 κ 1-39*01 5*01 0 0.423 "0.09411100 IgM 3-21*01 5*02 35 5 "0.361 "0.147 κ 3-11*01 4*01 0 0.129 "0.2871101 IgG 3-21*01 5*01 29 1 0.106 "0.284 λ 1-51*01 7*01 0 0.469 "0.0004361103 IgG 3-15*05 4*02 50 3 "0.0646 "0.0011 κ 1-12*01 5*01 0 "0.187 "0.007161105 IgM 3-48*02 3*02 43 1 "0.221 "0.0000286 λ 10-54*0 3*02 1 "0.299 "0.07171106 IgM 3-23*01 4*02 41 2 "0.217 "0.000117 κ 2-30*01 4*01 1 0.328 0.2411109 IgG 4-59*01 4*02 38 1 "0.467 "0.0603 λ 1-51*01 7*01 2 "0.25 "0.4911112 IgA 4-59*01 4*02 23 1 0.224 "0.0783 λ 2-11*01 2*01 0 0.213 "0.3581113 IgM 3-48*01 6*02 51 1 "0.133 "0.00191 κ 1-12*01 1*01 0 0.468 "0.2061114 IgG 3-21*01 4*02 36 2 0.0722 0.413 λ 4-69*02 2*01 0 0.479 "0.09251115 IgM 2-5*01 3*02 32 2 "0.0638 "0.0000247 κ 4-1*01 2*01 2 0.45 "0.05931118 IgG 3-15*05 6*02 69 0 "0.0359 "0.0101 κ 1-5*03 3*01 0 0.457 "0.0009621119 IgM 3-48*03 4*02 64 2 "0.288 "0.0000656 λ 1-40*01 7*01 0 "0.409 "0.003491120 IgM 1-18*01 4*02 39 0 "0.000776 "0.000000379 λ 1-47*01 3*02 0 "0.0294 "0.0005131121 IgG 3-11*03 5*01 35 1 "0.185 "0.071 κ 3-20*01 1*01 0 "0.138 "0.0000004431123 IgM 3-30*03 4*02 30 1 "0.176 "0.00237 κ 1-5*03 1*01 0 "0.227 "0.2151126 IgM 3-9*01 4*02 22 1 "0.32 "0.124 κ 3-15*01 4*01 0 0.0188 "0.161127 IgG 3-23*01 6*03 40 1 "0.0049 "0.000742 λ 1-44*01 3*02 0 "0.159 "0.02181130 IgM 4-b*02 5*02 23 1 "0.149 "0.0000498 λ 2-14*01 2*01 0 0.0266 "0.2651131 IgG 4-31*03 5*02 31 0 0.471 "0.248 λ 3-25*03 2*01 0 0.428 "0.03751132 IgM 3-7*01 4*02 34 0 0.195 "0.188 κ 1-39*01 1*01 1 0.018 "0.1761134 IgM 3-7*01 6*02 31 1 "0.348 "0.0404 κ 3-20*01 1*01 0 0.0251 "0.05651135 IgM 4-34*02 6*03 24 0 "0.218 "0.00563 λ 3-25*03 2*01 0 "0.324 "0.3421136 IgA 3-66*01 4*02 28 1 "0.0517 "0.0134 κ 3-15*01 4*01 0 "0.204 "0.06961137 IgG 3-74*03 4*02 23 1 "0.255 "0.0879 λ 1-40*01 3*02 0 "0.207 "0.08181139 IgM 3-48*02 4*02 37 1 "0.127 "0.00064 κ 1-39*01 1*01 0 "0.301 "0.01131141 IgM 3-48*01 5*02 36 1 "0.274 "0.00271 λ 3-21*02 3*02 0 "0.485 "0.01711142 IgM 3-21*02 4*02 31 1 0.375 "0.0482 λ 1-51*01 1*01 1 0.219 "0.009211143 IgA 4-4*07 3*02 19 0 0.064 "0.069 λ 1-51*01 7*01 0 "0.0383 "0.009211146 IgM 4-39*01 3*02 33 2 "0.186 "0.00626 κ 4-1*01 2*01 0 "0.0775 "0.00271148 IgA 3-11*01 5*02 31 2 "0.389 "0.00528 κ 1-39*01 4*01 0 0.466 "0.003961150 IgM 4-34*01 3*01 35 1 "0.409 "0.0941 λ 7-46*01 3*02 0 "0.0549 "0.01151151 IgG 4-39*01 6*03 44 2 0.344 "0.0146 λ 1-44*01 7*01 0 "0.0956 "0.00151153 IgG 3-7*01 3*02 44 2 "0.156 "0.00932 λ 1-51*02 3*02 0 "0.255 "0.04011154 IgM 4-61*02 1*01 23 1 "0.117 "0.0663 κ 4-1*01 4*01 0 "0.246 "0.09811155 IgM 4-34*01 4*02 11 0 "0.294 "0.0169 λ 1-51*01 3*02 0 0.175 "0.1151156 IgM 1-69*01 5*02 27 1 "0.133 "0.00526 λ 1-51*01 2*01 1 0.149 "0.1991158 IgM 3-48*01 4*02 25 1 "0.0954 "0.0653 κ 3-15*01 2*01 0 0.0874 "0.4051159 IgM 3-48*02 6*02 44 1 "0.105 "0.000955 κ 3-20*01 1*01 0 "0.45 "0.0005481160 IgM 3-48*02 4*02 31 1 "0.0143 "0.0049 κ 1-12*01 1*01 0 0.206 "0.01871162 IgM 3-48*02 6*02 24 1 "0.413 "0.0974 κ 3-20*01 4*01 0 "0.13 "0.0003641163 IgM 3-23*01 4*02 24 1 "0.286 "0.0117 λ 1-51*02 3*02 0 0.253 "0.02691164 IgM 3-23*01 4*02 34 1 "0.0272 "0.000986 κ 1-9*01 4*01 0 0.256 "0.4911165 IgM 3-53*01 3*02 31 2 "0.396 "0.00655 κ 3-15*01 4*01 0 "0.441 "0.01751166 IgG 3-48*02 4*02 18 0 "0.0491 "0.0408 κ 1-39*01 3*01 1 0.315 0.4581167 IgG 3-23*01 4*02 25 1 0.397 "0.0229 λ 2-14*01 3*02 0 "0.466 "0.01141168 IgA 3-48*03 3*01 23 1 0.21 "0.24 λ 4-69*01 7*01 0 "0.2 "0.09271170 IgM 3-11*01 4*02 40 3 "0.0409 "0.000703 κ 3-15*01 1*01 0 0.279 "0.03171171 IgM 3-9*01 4*02 48 1 "0.472 "0.0232 κ 3-20*01 1*01 0 "0.281 "0.01781172 IgM 3-48*02 6*02 38 3 "0.156 "0.00248 λ 1-51*01 7*01 0 "0.0981 "0.00003441173 IgG 4-59*01 5*02 37 5 "0.467 "0.0436 κ 1-39*01 4*01 1 0.357 "0.0161174 IgM 3-48*02 4*01 16 1 0.293 "0.0417 λ 3-9*01 1*01 0 "0.3 "0.0171175 IgG 3-53*01 6*02 83 1 0.417 "0.0207 κ 4-1*01 1*01 1 "0.165 "0.001161176 IgG 3-21*02 5*01 80 1 "0.011 "0.000597 κ 3-20*01 1*01 0 0.175 "0.0229

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Supplemental Figure 1. Mutational analysis of FL Igs ... · Framework regions (FWR1, FWR2, FWR3, and FWR4) and complementarity determining regions (CDR1, CDR2, and CDR3) are indicated