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Supplemental Figure Legends:
Figure 1S – Detailed topographic depiction of BlpHR6 with phenotype and fusion points marked.
Topology was predicted by TOPCONS and plotted using Protter on line tool. Amino acid shapes are
colored according to their phenotypic region after analyzing chimeras consisting of the N-terminus of
BlpHR6 joined to the C-terminus of BlpHP164. Circled amino acids are conserved between the two types,
squares are different. Arrows demonstrate points of chimera fusion. The G-box that is part of the
catalytic domain and surrounds the phosphorylated histidine is marked.
Figure 2S – Alignment of the six most common BlpH types and the naturally occurring chimera, BlpHP801.
Identical amino acids are marked with a dot, differences are noted and outlined in grey. The boxes
denote the areas found to represent areas involved in peptide specificity.
Figure 3S – Detailed topographic depiction of BlpH6A.3 with a summary of regions identified as playing
role in peptide specificity or response. Topology was predicted by TOPCONS and plotted using the
Protter on-line tool.
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different AA: P164 and R6region containing non-functional fusion pointsregion containing constitutively active fusion pointsBlpCR6 responsiveG boxphospo-H
shared AA:P164 and R6Supplemental Figure 1
Figure S2
MY I FW I I L Y T L I T NG L E I V I F F K V DG I G L T F E R I F K A F L L K I L L A F V F VM I G Y M I G N V Y L S Y F ME. N . V . . L . H . . . . . . . . . . . . . . . . . . N . . . . . . . . . . . . . . . . V . . . . . . S . I V . . . . . . . . . .. N V A . . L . . . . V . H . . K . . . . . . . . . . S . . . . . . . . . . . F . . . . . V . . . . . S . I V . . . . . . . . . .. N V A . . L . . . . V . H . . K . . . . . . . . . . S . . . . . . . . . . . F . . . . . V . . G . L . . . V . . . . . . . . . .. N . A . . L . . . . V . . . . . . . . . . . . . . . D . . . . . . . . . . . . . . . . . . . . . . . S . I V . . . . . . . . . .. N . A . . L . . . . V . . . . . . . . . . . . . . . D . . . . . . . . . . . . . F . . G I I . T T F Q F L A V S K . . . . . I .. N . A . . L . . A . V I . . . . . . . . . . . . . . . . . . D . . . . . . . . . F . . G I I . T T F Q F L A V S K . . . . . I .
P L Y G I G L S F L L L R G L P K K L L F F Y G L F P M I L V N L F Y R G V S Y F V L P F L GQ E Q V Y DG Y S F T G L C I I I F. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . G . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . G . . . . . . . . . . . . . . . .. . . . . . . . . . . . . E . . . . . . L . . . . . . . . . . . . . . . . . . . . . . . . . . . G . . . . D . . . I W . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . G . . . . . . . . . . . . . . . .. . F . . . . . . . . . . . . . . . I . I . . . . . . . . . . E . . . . . . . . . . . . . . . . G I . D G DGN P I F . L . M . .. . F . . . . . . . . . . . . . . . I . I . . . . . . . . . . E . . . . . . . . . . . . . . . . G I . D G DGN P I F . L . M . .
N F F I S L A F L KWL D Y D F T S L R R E I L D T G F Q K S L T K I NW I MG A Y Y L V I Q S L S Y F E Y E QG I Q S T T V R H. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . K G . . . K D . . . . . . Q . . . . . . . . . . . . . N . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . K . . . . K A . . . . . . Q . . . . . . G . . . . ME . . . F . . . . . S . . . K . . . .V C . . V . V . . . . . . . . . . R . . . . F . . . . . . . . . . . . . . A . . . . . . . M . . . . . L . . . . . . . . . . . . .V C . . V . V . . . . . . . . . . R . . . . F . . . . . . . . . . . . . . A . . . . . . . M . . . . . L . . . . . . . . . . . . .
L I L V F Y L L F F MG V I K K L D T Y L K D K L HDR L DQ E Q V L R Y R DME R Y S R H I E E L Y K E V R S F R HD Y T N L L. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . I . . . . . . . . . . . . . E . . N . . . D . . . . E . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . E . . Y E . . E . . . A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . G . . . . . . . . . E . . Q E E . N . . . T . . . . . . . . . . . . . . . . . . . I . . . . . . . . . . .. . . . . . . . . . . . G . . . . . . . . . E . . Q E E . N . . . T . . . . . . . . . . . . . . . . . . . I . . . . . . . . . . .
T S L R L G I E E E DME Q I K E V Y D L V L K D S S K K L QDN K Y D L G R L V N V R DR A L K S L L AG K F I K AR E K D I V. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . D . N . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . I . . S . . . . . . E . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . D . N . .. . . . . . . . . . . . . . . . . . . G S . . . . . . Q . . . N . . . . . . . . . . I . . K . . . . . . . . . . L . . . D . N . .. . . . . . . . . . . . . . . . . I . . S . . R . . . Q . . . . . . . . . . . . . . I . . . . . . . . . . . . . . . . . . . N . .. . . . . . . . . . . . . . . . . I . . S . . R . . . Q . . . . . . . . . . . . . . I . . . . . . . . . . . . . . . . . . . N . .
F N V E V P E E I Q V E GMR L L D F L T I V S I L C DN A I E A S A E AGQ P H V S I A F L K S G AQ E T F I I E N S I K E E G. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . V S . . . . . . V . . . . . . . . . . . . V . . C . . . . . . . . F . N . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . S . . . . . . . . . . . . . . . . . . . V . . S . . . . . . . . . . N . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . S . . . . . . . . . . . . . . . . . . . . . . S . . . . . . . . . . N . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . S . . . . . . . . . . . . . . . . . . . . . . S . . . . . . . . . . N . . . . . . . . . . . . . . . .
I D I S E I F S F G V S S K G E E R G V G L Y T VMK I V E S Y P N A S L N T T C QDQ V F R Q V L T M I P T E. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . A . . . . . . . . . . . . . . . . . . . . H . . T . . . . . . . . H . . . . . . . V . H I .. . . . . . . . . . A . . . . . . . . . . . . . . . . . . . . H . . T . . . . . . . N . . . . . . . . V . H . .. . . . . . . . . . A . . . . . . . . . . . . . . . . . . . . H . . T N . . . . . . N . . . . . . . . V . H A .. . . . . . . . . . A . . . . . . . . . . . . . . . . . . . . H . . T N . . . . . . N . . . . . . . . V . H A .
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Disruptive or decreased activity fusion pointsType 6A exclusionElectrostatic interactionSignal transferDifferences between type P164 and all type 6A responsive conservedDifferences between type 164 and all type 6A responsive non-conserved
Figure S3
WGS Strain blpC blpH blpA
Taiwan19F-14 6A 6A.1 DELETION
GA47628 6A 6A.1 DELETION
GA04375 6A 6A.1 DELETION
GA13499 6A 6A.1 DELETION
GA19451 6A 6A.1 DELETION
GA11856 6A 6A.1 DELETION
GA19923 6A 6A.1 DELETION
GA47778 6A 6A.1 DELETION
GA47688 6A 6A.1 DELETION
TCH8431/19A 6A 6A.1 DELETION
Canada MDR_19A 6A 6A.1 DELETION
Canada MDR_19F 6A 6A.1 DELETION
GA58771 6A 6A.1 DELETION
4075-00 6A 6A.1 DELETION
GA13455 6A 6A.1 DELETION
GA08825 6A 6A.1 DELETION
GA49138 6A 6A.1 DELETION
3063-00 6A 6A.1 DELETION
GA17719 6A 6A.1 DELETION
GA18523 6A 6A.1 DELETION
EU-NP02 6A 6A.1 DELETION
GA44288 6A 6A.1 DELETION
7533-05 6A 6A.1 DELETION
8190-05 6A 6A.1 DELETION
7879-04 6A 6A.1 DELETION
5185-06 6A 6A.1 DELETION
7286-06 6A 6A.1 DELETION
5652-06 6A 6A.1 DELETION
GA14798 6A 6A.1 DELETION
GA47281 6A 6A.1 DELETION
GA16833 6A 6A.1 DELETION
GA17371 6A 6A.1 DELETION
GA40028 6A 6A.2 DELETION
NP141 6A 6A.2 DELETION
CDC1087-00 6A 6A.2 DELETION
GA47283 6A 6A.2 DELETION
2070108 6A 6A.2 DELETION
2070109 6A 6A.2 DELETION
GA54354 6A 6A.2 DELETION
GA62331 6A 6A.2 DELETION
GA19101 6A 6A.1 FRAMESHIFT
SPN034156 6A 6A.1 FRAMESHIFT
GA47597 6A 6A.1 FRAMESHIFT
SPN072838 6A 6A.1 FRAMESHIFT
07AR0125 6A 6A.1 FRAMESHIFT
EU-NP04 6A 6A.1 FRAMESHIFT
TableS1 - WGS strains with deduced blpC and blpH type and blpA sequence specifics
GA60190 6A 6A.1 FRAMESHIFT
GA52306 6A 6A.1 FRAMESHIFT
GA47033 6A 6A.1 FRAMESHIFT
2071247 6A 6A.2 FRAMESHIFT
2081685 6A 6A.2 FRAMESHIFT
GA02254 6A 6A.2 FRAMESHIFT
BS69 SP14 6A 6A.2 FRAMESHIFT
GA13494 6A 6A.2 FRAMESHIFT
2070035 6A 6A.3 FRAMESHIFT
2080076 6A 6A.3 FRAMESHIFT
BS73 SP6 6A 6A.1 INTACT
SPNA45 6A 6A.2 INTACT
GA60080 6A 6A.3 INTACT
England14-9 6A 6A.3 INTACT
INV200 6A 6A.3 INTACT
GA41688 6A 6A.3 INTACT
BS458 6A 6A.3 INTACT
BS292 6A 6A.3 INTACT
BS397 6A 6A.3 INTACT
BS293 6A 6A.3 INTACT
BS457 6A 6A.3 INTACT
CGSP14 6A 6A.3 INTACT
GA13723 6A 6A.3 INTACT
GA47976 6A 6A.3 INTACT
GA13338 6A 6A.3 INTACT
BS455 6A 6A.3 INTACT
GA58581 6A 6A.3 INTACT
2061376 6A 6A.3 INTACT
2090008 6A 6A.3 INTACT
GA56348 6A 6A.3 INTACT
GA58981 6A 6A.3 INTACT
2082239 6A R6 INTACT
2061617 6A R6 INTACT
GA47373 P164 P801 DELETION
GA07643 P164 P164 FRAMESHIFT
GA47360 P164 P164 FRAMESHIFT
GA18068 P164 P164 FRAMESHIFT
EU-NP01 P164 P164 FRAMESHIFT
SPAR48 P164 P164 FRAMESHIFT
BR1064 P164 P164 FRAMESHIFT
ND6012 P164 P164 FRAMESHIFT
GA07914 P164 P164 INTACT
GA47760 P164 P164 INTACT
AP200 P164 P164 INTACT
BS70 SP11 P164 P164 INTACT
GA47562 P164 P164 INTACT
MLV-016 P164 P164 INTACT
P1031 P164 P164 INTACT
SPN1041 P164 P164 INTACT
SPN74765 P164 P164 INTACT
GA13224 P164 P164 INTACT
GA16121 P164 P164 INTACT
670-6B P164 P164 INTACT
GA05248 P164 P164 INTACT
GA02506 P164 P164 INTACT
GA41538 P164 P164 INTACT
GA16242 P164 P164 INTACT
NP112 P164 P164 INTACT
GA05578 P164 P164 INTACT
GA04216 P164 P164 INTACT
GA11304 P164 P164 INTACT
BS74 SP18 P164 P164 INTACT
GA17545 P164 P164 INTACT
GA47901 P164 P164 NONSENSE
SPN032672 P164 P164 NONSENSE
SPN033038 P164 P164 NONSENSE
SPN061370 P164 P164 NONSENSE
GA16531 P164 P164 INTACT
BS75 SP19 (P801) R6 P801 DELETION
GA07228 R6 R6 FRAMESHIFT
BS71 SP3 R6 R6 FRAMESHIFT
SPN021198 R6 R6 FRAMESHIFT
SPN034183 R6 R6 FRAMESHIFT
GA19690 R6 R6 FRAMESHIFT
OXC141 R6 R6 FRAMESHIFT
SPN994038 R6 R6 FRAMESHIFT
SPN994039 R6 R6 FRAMESHIFT
GA41317 R6 R6 FRAMESHIFT
GA49194 R6 R6 FRAMESHIFT
EU-NP03 R6 R6 FRAMESHIFT
BS72SP23 R6 R6 FRAMESHIFT
R6 R6 R6 FRAMESHIFT
D39 R6 R6 FRAMESHIFT
CDC0288-04 R6 R6 FRAMESHIFT
GA05245 R6
AAs 1-248R6/ 249-
360 type6A.1/361-
endT4
FRAMESHIFT
SPAR27 R6 R6 FRAMESHIFT
GA47439 R6 R6 FRAMESHIFT
70585 R6 R6 FRAMESHIFT
GA17227 R6
AAs 1-248R6/ 249-
360 6A.1/ 361-end
T4
FRAMESHIFT
GA41301 R6
AAs 1-248R6/ 249-
360 6A.1/ 361-end
T4
FRAMESHIFT
2080913 R6 R6 FRAMESHIFT
GA44128 R6 R6 FRAMESHIFT
NP070 R6 R6 FRAMESHIFT
2070005 R6 R6 FRAMESHIFT
2070768 R6 R6 FRAMESHIFT
GA14688 R6 R6 FRAMESHIFT
2072047 R6 R6 FRAMESHIFT
CCRI 1974 R6AA 1- 147 R6/148
to end T4FRAMESHIFT
GA08780 R6 T4 FRAMESHIFT
SP195 R6 T4 FRAMESHIFT
GA17570 R6 T4 FRAMESHIFT
GA43257 R6 T4 FRAMESHIFT
GA54644 R6 T4 FRAMESHIFT
GA56113 R6 T4 FRAMESHIFT
GA17301 R6 T4 FRAMESHIFT
2070531 R6 T4 FRAMESHIFT
BS68 SP9 R6 T4 FRAMESHIFT
2070425 R6 T4 FRAMESHIFT
GA49542 R6 T4 FRAMESHIFT
GA44511 R6 T4 FRAMESHIFT
GA19998 R6 T4 FRAMESHIFT
GA47388 R6 T4 FRAMESHIFT
GA47751 R6 6A.2 INTACT
CDC1873-00 R6 R6 INTACT
GA41437 R6 R6 INTACT
NorthCarolina6A-23 R6 R6 INTACT
GA02714 R6 R6 INTACT
NP127 R6 R6 INTACT
GA14373 R6 R6 INTACT
GA17328 R6 R6 INTACT
GA19077 R6 R6 INTACT
SPAR55 R6 R6 INTACT
GA47368 R6 R6 INTACT
GA02270 R6 R6 INTACT
GA41410 R6 R6 INTACT
GA43264 R6 R6 INTACT
GA44386 R6 R6 INTACT
GA44452 R6 R6 INTACT
GA49447 R6 R6 INTACT
Hungary19A-6 R6 R6 INTACT
SV35 T4
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P164/ 363-end
T4
FRAMESHIFT
GA13856 T4 T4 FRAMESHIFT
2070335 T4 T4 FRAMESHIFT
GA13637 T4 T4 FRAMESHIFT
GA47502 T4 T4 FRAMESHIFT
TIGR4 T4 T4 FRAMESHIFT
2082170 T4 T4 FRAMESHIFT
2081074 T4 T4 FRAMESHIFT
GA40563 T4 T4 FRAMESHIFT
GA17971 T4 T4 FRAMESHIFT
PT8114 T4 T4 FRAMESHIFT
GA60132 T4 T4 FRAMESHIFT
JJA T4 T4 FRAMESHIFT
GA11663 T4 T4 FRAMESHIFT
GA41565 T4 T4 FRAMESHIFT
NP170 T4 T4 FRAMESHIFT
SV36 T4 T4 FRAMESHIFT
459-5 T4 T4 FRAMESHIFT
ATCC 700669 T4 T4 FRAMESHIFT
GA44378 T4 T4 FRAMESHIFT
CDC3059-06 T4 T4 FRAMESHIFT
GA17457 T4 T4 FRAMESHIFT
GA41277 T4 T4 FRAMESHIFT
GA04175 T4 T4 FRAMESHIFT
GA47522 T4 T4 FRAMESHIFT
Netherlands15B-37 T4 T4 FRAMESHIFT
GA04672 T4 T4 FRAMESHIFT
GA62681 T4 T4 FRAMESHIFT
GA13430 T4 T4 FRAMESHIFT
GA11184 T4 T4 FRAMESHIFT
GA40183 T4 T4 FRAMESHIFT
GA11426 T4 T4 FRAMESHIFT
GA44500 T4 T4 FRAMESHIFT
4027-06 T4 T4 FRAMESHIFT
GA43380 T4 T4 FRAMESHIFT
6735-05 T4 T4 FRAMESHIFT
6901-05 T4 T4 FRAMESHIFT
6963-05 T4 T4 FRAMESHIFT
5787-06 T4 T4 FRAMESHIFT
GA43265 T4 T4 FRAMESHIFT
GA40410 T4 T4 FRAMESHIFT
GA06083 T4 T4 FRAMESHIFT
EU-NP05 T4 T4 FRAMESHIFT
GA47210 T4 T4 FRAMESHIFT
2071004 T4 T4 FRAMESHIFT
GA47461 T4 T4 FRAMESHIFT
GA17484 T4 T4 FRAMESHIFT
INV104 T4
AA1-316
P164/317-end
T4
INTACT
G54 T4 T4 INTACT
GA47179 T4 T4 INTACT
GA47794 T4 T4 INTACT
GA44194 T4 T4 INTACT
GA52612 T4 T4 INTACT
WGS strains: BlpC and BlpH designations as in text. Naturally
occurring chimeras noted in orange with details on fusion points.
