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Supplemental Figure S1. Amounts of sucrose (A,F), Glu (B,G), Fru (C,H), Fru 6-P (D,I) and starch (E,J) in WT tobacco and three transgenic lines with altered AOX amount, in both well- watered plants (A-E) and moderate drought-stressed plants (F-J). Data are the mean + S.E. of three independent experiments. Within a treatment, no significant differences were found across plant lines for any of these metabolites.
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Supplemental Figures S1-S8
Supplemental Figure S1
B7 WTRI9
RI29
0.00
0.06
0.12
0.18
A
Sucr
ose
(um
ol m
g-1
DW
)
B7 WTRI9
RI29
0.00
0.08
0.16
0.24
B
Glu
(um
ol m
g-1
DW
)
B7 WTRI9
RI29
0.00
0.06
0.12
0.18
C
Fru
(um
ol m
g-1
DW
)
B7 WTRI9
RI29
0.00
0.01
0.02
D
Fru
6-P
(um
ol m
g-1
DW
)
B7 WTRI9
RI29
0.0
0.1
0.2
0.3E
Star
ch(u
mol
Glu
mg
-1 D
W)
B7 WT RI9RI29
0.00
0.06
0.12
0.18
F
Sucr
ose
(um
ol m
g-1
DW
)
B7 WT RI9RI29
0.00
0.08
0.16
0.24
G
Glu
(um
ol m
g-1
DW
)
B7 WT RI9RI29
0.00
0.06
0.12
0.18
H
Fru
(um
ol m
g-1 D
W)
B7 WT RI9RI29
0.00
0.01
0.02
I
Fru
6-P
(um
ol m
g-1
DW
)
B7 WT RI9RI29
0.0
0.1
0.2
0.3J
Star
ch(u
mol
Glu
mg
-1 D
W)
Supplemental Figure S1. Amounts of sucrose (A,F), Glu (B,G), Fru (C,H), Fru 6-P (D,I) and
starch (E,J) in WT tobacco and three transgenic lines with altered AOX amount, in both well-
watered plants (A-E) and moderate drought-stressed plants (F-J). Data are the mean + S.E. of
three independent experiments. Within a treatment, no significant differences were found
across plant lines for any of these metabolites.
Supplemental Figure S2
0 500 1000 1500 2000
0
3
6
9
12A
PPFD (mol m-2 s-1)
An
(m
ol C
O2 m
-2 s
-1)
0 500 1000 1500 2000
0
40
80
120
160
B
PPFD (mol m-2 s-1)
ETR
II
(m
ol e
- m-2
s-1
)
0 500 1000 1500 2000
0.0
0.2
0.4
0.6
C
PPFD (mol m-2 s-1)
1-qP
0 500 1000 1500 2000
0.00
0.75
1.50
2.25
D
PPFD (mol m-2 s-1)
NPQ
0 500 1000 1500 2000
0
2
4
6
8E
PPFD (mol m-2 s-1)
An
(m
ol C
O2 m
-2 s
-1)
0 500 1000 1500 2000
0
30
60
90
120F
PPFD (mol m-2 s-1)
ETR
II
(m
ol e
- m-2
s-1
)
0 500 1000 1500 2000
0.0
0.2
0.4
0.6
0.8
1.0G
PPFD (mol m-2 s-1)1-
qP
0 500 1000 1500 2000
0
1
2
3
4H
PPFD (mol m-2 s-1)
NPQ
Supplemental Figure S2. Light response curves for An (A,E), ETRII (B,F), excitation pressure
(C,G) and NPQ (D,H) in WT tobacco (closed circles) and three transgenic lines (B7, open
circle; RI9, open triangle; RI29, open square) with altered AOX amount, in both well-watered
plants (A-D) and moderate drought-stressed plants (E-H). Data are the mean + S.E. of three
independent experiments.
Supplemental Figure S3
0 200 400 600 800
0
5
10
15
20A
Ci (mol CO2 mol-1)
An
(m
ol C
O2 m
-2 s
-1)
0 200 400 600 800
100
150
200B
Ci (mol CO2 mol-1)
ETR
II
(m
ol e
- m-2
s-1
)
0 200 400 600 800
0.0
0.2
0.4
0.6
0.8
C
Ci (mol CO2 mol-1)
1-qP
0 200 400 600 800
1.0
1.5
2.0
2.5
3.0D
Ci (mol CO2 mol-1)
NPQ
Supplemental Figure S3. CO2 response curves for An (A), ETRII (B), excitation pressure (C)
and NPQ (D) of WT tobacco in both well-watered (open circles) and moderate drought-
stressed (closed circles) plants. Data are the mean + S.E. of three independent experiments.
These are re-plotted data from Fig.’s 2 and 3.
Supplemental Figure S4
0 500 1000 1500 2000
0
5
10
A
PPFD (mol m-2 s-1)
An
(m
ol C
O2 m
-2 s
-1)
0 500 1000 1500 2000
0
40
80
120
160
B
PPFD (mol m-2 s-1)
ETR
II(
mol
e- m
-2 s
-1)
0 500 1000 1500 2000
0.0
0.2
0.4
0.6
0.8C
PPFD (mol m-2 s-1)
1-qP
0 500 1000 1500 2000
0
1
2
3
D
PPFD (mol m-2 s-1)
NPQ
Supplemental Figure S4. Light response curves for An (A), ETRII (B), excitation pressure (C)
and NPQ (D) of WT tobacco in both well-watered (open circles) and moderate drought-
stressed (closed circles) plants. Data are the mean + S.E. of three independent experiments.
These are re-plotted data from Fig. S3.
0 500 1000 1500 2000
0.00
0.25
0.50
0.75
1.00
A
PPFD (mol m-2 s-1)Y(
I)
0 500 1000 1500 2000
0
25
50
75B
PPFD (mol m-2 s-1)
CET
(m
ol e
- m-2
s-1
)
Supplemental Figure S5
Supplemental Figure S5. Photochemical quantum yield of PSI [Y(I)](A) and the relative rate
of CET around PSI (B) in WT tobacco in both well-watered (open circles) and moderate
drought-stressed (closed circles) plants. Data are the mean + S.E. of three independent
experiments. These are re-plotted data from Fig.’s 5 and 6.
MitochondriaChloroplast
Well-watered Drought Well-watered Drought
Supplemental Figure S6
1 0.419 0.338 0.264
Supplemental Figure S6. Immunoblot analysis of AtpB amount in separate chloroplast and
mitochondrial fractions isolated from either well-watered or moderate drought-stressed AOX
knockdown (RI29) plants. Equal amounts of total protein (10µg) were loaded in each lane.
Numbers below the blot indicate relative signal density of the different samples.
Supplemental Figure S7
B7 WTRI9
RI29 B7 WTRI9
RI29
0.0
0.4
0.8
1.2
Well-watered Drought
A
FeSO
D2
(rel
ativ
e ac
tivity
)
B7 WTRI9
RI29 B7 WTRI9
RI29
0.0
0.5
1.0
1.5
Well-watered Drought
B
CuZ
nSO
D2
(rel
ativ
e ac
tivity
)
Supplemental Figure S7. Maximal activities of the minor SOD isoforms FeSOD2 (A) and
CuZnSOD2 (B) in WT tobacco and three transgenic lines with altered AOX amount, in both
well-watered and moderate drought-stressed plants. Data are the mean + S.E. of six
independent experiments. Activities are relative to that of the well-watered WT activity,
which was set to 1. Within a treatment, no significant differences were found in either SOD
activity across the different plant lines.
Supplemental Figure S8
A B
Supplemental Figure S8. A representative example of CO2 response curves at low Ci and
three different PPFD (A) and a light response curve at low PPFD (B). Such data were used to
estimate RL by either the Laisk method (A) or the Kok method (B), as described in Materials
and Methods.
