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B LeafShootFlowerRootMGRRB WT SlHDC10-FL C GUS Activity (nmoles 4MU/hr/mg protein) GUS NOS 5' +1 SlHDC10-Fl GUS -968 NOS SlHDC10-DI +1 GUS -668 NOS SlHDC10-DII +1 A CArG box Nitrate responsive element Ethylene responsive element Ethylene biosynthesis element Supplementary Fig. S3: (A) Scheme of SlHDC10 full length and deletion GUS reporter constructs. Presence of different important cis-regulatory elements and their subsequent deletions in the deletion constructs are shown in figure. (B) & (C) Histochemical analysis of GUS activity in different tissues of transgenic tomato lines. For histochemical assay of three independent transgenic lines were used and data of a representative line is presented. For fluorimetric analysis at least three independent transgenic lines which gave GUS staining were taken and three biological replicate for each line were utilized and average of fluorimetry reading were taken for calculation.
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Supplementary Fig. S1: GO classification of 2,318 differentially expressed genes during fruit ripening in tomato. The genes which show differential expression in at least one stage, out of the total four different ripening transition phases (Kumar et al., 2012), including mature green versus breaker, breaker versus breaker+3, breaker+3 versus breaker+7 and breaker+7 versus breaker+20 in wild type were used for the study. All the differentially expressed genes (p value≤0.05, FC≥2 fold) were sorted for unique gene list. The differentially expressed genes were used for GO classification, based on biological process, using tomato functional genomics database (http://ted.bti.cornell.edu/).The output was use to make the final graph using MS excel. Functional categories with at least 50 or more genes were used to make final graph.
SlAAD3:C
aAAD3
SlAAD4:C
aAAD4
SlGAD
4:CaG
AD4, C
aGAD
5SlH
DC
1:CaH
DC
1SlH
DC
9:CaH
DC
11SlH
DC
15:CaAAD
5SlG
AD1:C
aGAD
3
Supplementary Fig. S2: Syntelog analysis between tomato and pepper. The analysis was performed as described in earlier.
B Leaf Shoot FlowerRoot MG RRB
WT
SlH
DC
10-F
L
C
0
5
10
15
20
25
Leaf Root Shoot Flower MG Br RR
WT L1 L2 L3
GU
S A
ctiv
ity
(nm
oles
4M
U/h
r/mg
prot
ein)
GUS-1599
NOS5'
+1SlHDC10-Fl
GUS-968
NOSSlHDC10-DI
+1
GUS-668
NOSSlHDC10-DII
+1
A
CArG box
Nitrate responsive element
Ethylene responsive element
Ethylene biosynthesis element
Supplementary Fig. S3: (A) Scheme of SlHDC10 full length and deletion GUS reporter constructs. Presence of different important cis-regulatory elements and their subsequent deletions in the deletion constructs are shown in figure. (B) & (C) Histochemical analysis of GUS activity in different tissues of transgenic tomato lines. For histochemical assay of three independent transgenic lines were used and data of a representative line is presented. For fluorimetric analysis at least three independent transgenic lines which gave GUS staining were taken and three biological replicate for each line were utilized and average of fluorimetry reading were taken for calculation.
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