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Supplementary Fig.1 The overall workflow
0-1 1 3 5 13 15 20 40 60Day
Cell plating
7 4~5 days
CHIRActivinA
BMP4 XavMGCHIR
Induced differentiation
PMOEndo-Porter
Exon skipping
RT-PCR, Dystrophin WB, Immunocytochemistry
Ca imaging
E8 RPMI/B27-ins
Single cell replating
RT-PCR
RPMI/B27
Control Patient
OCT4
SOX2
NANOG
β-actin
A B
CDAPI NANOG SSEA-4/DAPIOCT3/4
Supplementary Fig.2 Characterization of established DMD-iPSCs
Neuroepithelium Cartilage Intestine
D
+ S.C. + PMO 10 μM
Δ46-55 + S.C. + PMO 5 μM + PMO 10 μM
500
Supplementary Fig.3 Skipping efficiency of PMO in short and long term culture
A
B
500
1000
1000
690 bp
514 bp
44 45 56 5743
44 56 5743
690 bp
514 bp
44 45 56 5743
44 56 5743
BD FACSDiva 8.0
Global Sheet1 Printed on: 7 9, 2018 06:25:26 JSTPage 1 of 1
Supplementary Fig.4. Flow cytometry for cardiomyocyte differentiation of human iPSCs
BD FACSDiva 8.0
Global Sheet1 Printed on: 7 9, 2018 06:25:36 JSTPage 1 of 1
Cel
l cou
nt
cTnT-PE
Cel
l cou
nt
Isotype IgG-PE