Supplementary Figure Legends:

Table 1. List of primers used for Real Time – PCR

Supp Fig 1. S100A7-overexpressing MCF7 cells show decreased formation of migratory structures compared to vector control. Phase contrast image of cultured MCF7/Vec and MCF7/S100A7 cells taken at 10x magnification. Inset image shows the representative zoomed area.

Supp Fig 2. S100A7-overexpression in MCF7 cells down-regulate genes which are involved in cancer pathways. Total RNA from MCF7/Vec and MCF7/S100A7 cells were analyzed by Affymetrix Human Genome U133 chip. (A) Gene ontology studies using IPA analysis of microarray data. (B) Heat-map of differentially expressed genes in MCF7/Vec and MCF7/S100A7 cells showing reduced expression of β-catenin/TCF4 pathway genes.

Supp Fig 3. Expression of estrogen receptor alpha (ERα) in S100A7-overexpressing and vector control MCF7 and T47D cells. 50 μg cell lysates of MCF7/Vec, MCF7/S100A7, T47D/Vec and T47D/S100A7 cells were subjected to Western blotting with ERα antibody. GAPDH was used as a loading control in all the blots.

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Supplementary figures

Gene Primer sequence

GSK3β Forward (5’ GGTCTATCTTAATCTGGTGCTGG 3’)Reverse (5’ TGGATATAGGCTAAACTTCGGAAC 3’)

Cyclin D1 Forward (5’ TATTGCGCTGCTACCGTTGA 3’)Reverse (5’ CCAATAGCAGCAAACAATGTGAAA 3’)

E-cadherin Forward (5’ AGGCCAAGCAGCAGTACATT 3’)Reverse (5’ ATTCACATCCAGCACATCCA 3’)

β-catenin Forward (5’ GCTGGGACCTTGCATAACCTT 3’)Reverse (5’ ATTTTCACCAGGGCAGGAATG 3’)

GAPDH Forward (5’ ACCCACTCCTCCACCTTTG 3’)Reverse (5” CTCTTGTGCTCTTGCTGGG 3’)

Table 1. List of primers used for Real Time – PCR

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MCF7/Vec MCF7/S100A7

Supp. Fig. 1

4

Supp. Fig. 2.

TCF7L2 TCF7L2 TCF7L2 DVL2 DVL3 APC APC CCND1 CCND1 ANAPC5 GSK3β DVL2 AXON1 CTNNB1 CTNNB1 ANAPC4

10.0 7.0 4.0MCF7/Vec MCF7/S100A7

A B

5

Vec S100A7MCF7

ER-α

GAPDH

ER-α

GAPDH

Vec S100A7

T47D

Supp. Fig. 3.