0.69 IWBX op ANEI XJD llwrBImm~-mIB13-m mt?mHmQuIN~~AH)-cHATNKglolLcas AFlxRaPmEiE?m- l&r&_L, Takala J., Kari A., Huttunen K., Hutbnen H. Critical Care Research Program & Dept. of Surgery, Kuopio University Central Hospital , Kuopio , Finland

Open heart surgeq induces hypermtabolism and increases N-loss. Me studied 12 patients after coronary artery by-pass surgery receiving either glucose (G), gluc- ose and conventional amino acids (CAA) or glucose and E?CAA enriched solution (=w * The leg uptake and plasma concentrations of HCAA and BCKA ware measur- ed: After rwarming (M 1) and 4 hours after rwarming (M 2). The infusions were started after arrival to ICLJ. when rewanwd the ECAA content was increased tc 35% in HCAA group and 2 hours thereafter to 50%. In the CAA group the nitrogen intake was increased similarly b.& the HCAA content was kept 20 %.

rn1 I m 21 Ile KIM r&u mc Ile KIM Ieu KIC

G (n=4) 12~3 6.1Ll.8 72~15 3129 15+5 1222 60217 39+10 CAA (n=4) 53~16 2022 109229 47212 86~10 2829 162~6.7 51212 HCAA (n=4) 449 26~8 116210 41~17 147227 22d 268248 66212 Uff aID<0.05. . u a GvsCAAvszAA m GWCAAVSHCAA &L EAAvsG u a GvsCAAvsBCAA , CAAVSHCAA m GvsCAAvsHCAA , CAAVSBCAA l&& GvsCAAvsHCAA , CAAVSHCAA m GvsBCAA, CAAVSBCAA . Valine concentrations increased in CAA and BCAA groups la& the concentrations of KIV remained on control level. The leg uptake of leucine increased temporarily in CAAandHCAAgroups, tit returned later ( M 2 ) to control level. The leg output of KIC was greatest in HCAA group 4 hours after rewarming (G:-0.3~0.1,CAA:-0.012 0.2*, NCAA:-0.44~0.2, *p<O.O5 CAA vs HCAA). coaclusions Increased HCAA intake during early postoperative phase increases PCAA transamination and leg release of ketoacids in direct relation to input without any effect on leg uptake of amino acids.

0.70 SYNTHETIC HUMAN GROWTH HORMONE SPARES NITROGEN AND IMPROVES MUSCLE PROTEIN METABOLISM IN HEALTHY SUBJECTS ON A HYPONITROGENOUS

A. von der Decken, J. Wernerman, E. Vinnars and Intensive Care at St Goran’s Hos

Intitute for l? ital, Dande d’s Hos ital, and

xperimental % FJniversity of iology,

Provision of synthetic human growth hormone (HGH) to catabolic patients is reported to improve nitrogen balance and stimulate wound healing. The basis for these effects is suggested to be an im roved nitrogen economy. To establish a baseline for future clinical studies the effects of synthetic H8H on muscle metabolism and whole body nitrogen metabolism were evaluated in healthy subjects on a normocaloric hyponitrogenous diet.

Methods: Healthy volunteers (n= 12) were given an oral diet containing 165 kJ/kg bw/24 h and 0.05 g N/kg bw/24 h for 15 days. After a conditionin eriod intramuscular in’ections of syntetic HGH (0.1 !P

the HGH group (n = 6) received daily

received saline. & E/kg bw) during days 7- 15, a control group (n = 6)

arly serum samples and urine in 24 h portions were collected for urea determination. Percutaneous muscle biopsies were taken on days 0,7, and 15 for analysis of the free amino acid concentrations and the concentration and size distribution of ribosomes as an estimation of muscle protein synthesis.

Results: Urinary urea excretion and serum urea concentration were lower in the HGH group as compared to controls during the treatment period (P < 0.01). The concentrations of several individual amino acids in skeletal muscle among them alanine and glutamine were lower in the control group as compared to the HGH grou on da day 15 in the control group P 5v

15 (P < 0.05). The concentration of polyribosomes declined on P < 0.0 ) but not in the HGH group as compared to dag 0.

Concl& During a h onitrogenous diet synthetic HGH improved whole body nitrogen economy and had a favourable m uence on skeletal muscle protein metabolism in healthy subjects. 3

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