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5
Adaptador
OH-OH-
-OH-OH
P-HO- -P
-OH5’
3’ 5’
3’OH-
-OH-OH
OH-
Fragmento de DNA genómico
ApoAlert LM-PCR (Clontech)
T4 DNA Ligasa
72ºC 3 min
Taq DNA polimerasa
PCR
21
http://www.stratagene.com/cellbio/apoptosis/rt_pcr.htmhttp://www.clontech.com/archive/JUL01UPD/pdf/Apoptosis.pdfhttp://www.maximbio.com/mbi65.htmhttp://www.biocompare.com/molbio.asp?catid=1460http://www.biosite.se/kunder/biosite/
Análisis de los mecanismos moleculares de la apoptosis mediante RT-PCR
Medida de la apoptosis a nivel de mRNA
Principles of Amplifluor™ Apoptosis Gene Systems
http://www.intergenco.com/body_apoptosis_amplifluor.html
reporter
quencher
Real-time DetectionInstruments
Endpoint DetectionInstruments
Fluorescence Plate Reader
Spectrofluorometer
UV transilluminator/camera
Fluoroimaging gel systems