th Analytical Bioanalytical Techniques...5th International Conference and Ehibition on Analytical Bioanalytical Techniques August 18-20, 2014 DoubleTree by Hilton Beijing, China 196th

5th International Conference and Exhibition on

Analytical & Bioanalytical TechniquesAugust 18-20, 2014 DoubleTree by Hilton Beijing, China

196th OMICS Group Conference

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Analytica Acta-2014

Scientific Tracks & AbstractsDay 1

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Analytica Acta-2014

Day 1 August 18, 2014

1: Novel Approaches to Analytical & Bioanalytical Methods2: Analytical Methodology

Track 1 & 2

Session ChairDoo Soo ChungSeoul National University, Korea

Session Co-ChairL A FrankInstitute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Russia

Title: Chemiluminescent determination of hydrogen peroxide using FeIII-TAML activator, a potent peroxidase mimicking enzymeMarina M Vdovenko, Lomonosov Moscow State University, Russia

Title: Thin film microextraction of VOCs from biological samples using PDMS/ZSM-5 hybrid adsorbentsSeung-Woo Lee, The University of Kitakyushu, Japan

Title: Mapping single DNA molecules to the human genome in a nanofluidic deviceRodolphe Marie, Technical University of Denmark, Denmark

Title: Electrochemical determination of pyrogallol at conducting poly(3,4-ethylenedioxothiophene) film-modified screen-printed carbon electrodesShu-Hua Cheng, National Chi Nan University, Taiwan

Title: Coelenterazine-dependent bioluminescent proteins as effective reporters for in vitro assayL A Frank, Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Russia

Title: Liquid extraction surface analysis coupled with capillary electrophoresis to determine organophosphorous pesticides on appleDoo Soo Chung, Seoul National University, Korea

Title: Measuring viscosity on the nanoscale using fluorescent molecular rotorsAndrew C Benniston, Newcastle University, UK

Title: Supramolecular and nanobiomimetic approach to optimization of analytical reactionsSergei Shtykov, Saratov State University, Russia

Title: Simultaneous determination of phthalate esters in a microfluidic device coupled with an electrochemical sensorYoon-Bo Shim, Pusan National University, S. Korea

Title: Optical Si-based biosensors: First resultsSebania Libertino, Istituto per la Microelettronica e Microsistemi (CNR-IMM), Italy

Title: Some trends in analytical chemistry developmentYuri A Zolotov, Lomonosov Moscow State University, Russia

Title: Development and validation of HPTLC method for simultaneous estimation of olmesartan medoxomil and indapamide in tablet dosage formCelina Nazareth, PES’s Rajaram and Tarabai Bandekar College of Pharmacy, India

Title: Sampling gaseous compounds of heating essential oil using solid phase microextraction devicesWen-Hsi Cheng, Fooyin University, Taiwan

Title: Using of short chain alkyl imidazolium ionic liquids in enhancing the sensitivity of capillary electrophoresisDeia Abd El-Hady, King Abdulaziz University, Saudi Arabia

Title: Fluorescence switch for selectively sensing Copper (II) and L-Histidine in vitro and in living cellsGaolin Liang, University of Science and Technology of China, China

Title: An in situ liquid cell TEM study on nanomaterial depositions and nano characterizationsXin Chen, East China University of Science and Technology, China

Title: Design of nanoprobes for in situ analysis of cellular functional biomoleculesHuangxian Ju, Nanjing University, P. R. China

Session Introduction

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Volume 5, Issue 4J Anal Bioanal Tech 2014

ISSN: 2155-9872, JABT an open access journalAnalytica Acta-2014

August 18-20, 2014

August 18-20, 2014 DoubleTree by Hilton Beijing, China


Analytical & Bioanalytical Techniques

Marina M Vdovenko et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Chemiluminescent determination of hydrogen peroxide using FeIII-TAML activator, a potent peroxidase mimicking enzymeMarina M Vdovenko, Alexandra S Demiyanova, Kirill E Kopylov and Ivan Yu SakharovLomonosov Moscow State University, Russia

Efforts to replace native peroxidase with its low molecular weight alternatives have stimulated a search for peroxidase mimetics. Herein we describe the oxidation of luminol with hydrogen peroxide catalyzed by commercial available FeIII-

TAML activator 1a, which was showed to be more active catalyst than hemin. At FeIII-TAML activator 1a use in chemiluminescent assay for H2O2 determination the limit value (3σ) was 5x10-8 M, whereas in the presence of hemin the detection limit was significantly higher and equal to 6x10-7 M. The linear ranges (R2=0.98) of the assay were 6x10-8-1x10-6 M and 6x10-7-1x10-6 M H2O2 for FeIII-TAML 1a and hemin, respectively. The CV values for FeIII-TAML 1a-based assay measured within the working range varied from 1.0 to 3.7% (n=4), whereas in the case of hemin -5.0 to 9.7% (n=4). Moreover, the sensitivity of FeIII-TAML 1a-based method was 56 times higher than that of hemin-based method. The obtained results open good perspectives to apply FeIII-TAML activator 1a in CL analytical methods instead of hemin, traditionally used peroxidase mimetic.

BiographyMarina M Vdovenko is a Scientific Researcher at the Department of Chemistry, Lomonosov Moscow State University (Russia). She has graduated with PhD degree in Biotechnology in 2011 under Prof. Ivan Yu Sakharov. Presently her work focuses on the development of novel sensitive chemiluminescent methods for their use in analytical practice. She has published more than 15 papers in peer-reviewed journals.

[email protected]

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August 18-20, 2014




Seung-Woo Lee et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Thin film microextraction of VOCs from biological samples using PDMS/ZSM-5 hybrid adsorbentsSeung-Woo Lee1, Tao Wang1, Shigemi Goda1, Roman Selyanchyn1 and Hidetaka Matsui21The University of Kitakyushu, Japan2Shinkou Seiki Co. Ltd., Japan

Thin film microextraction (TFME) approach recently aroused in the scientific literature as one of the alternative to the solid phase microextraction (SPME) based on the fiber geometry. Generally, in theory, not different from SPME, TFME

possesses several important advantages. Since the amount of analytes extracted in SPME is proportional to the volume of the extraction phase, the sensitivity of a method can be improved by increasing the volume of the extraction phase. In general, this extraction approach exhibits much higher extraction rates than SPME fiber due to the higher surface area to extraction phase volume of the thin film. In this technique, scientists used a thin sheet of PDMS membrane attached to a deactivated stainless steel rod like a flag.

In current research, we investigated a class of hybrid materials of zeolite and polydimethylsiloxane. Such hybrid materials have been previously reported for several tasks, in particular, selective filtration due to size exclusion mechanism. However, these composites were not thoroughly studied for the chemicals sorption, retention and controlled release purposes. They not only possess longer retention of targeted analytes due to size-fitting on the zeolite pores but also can be used for enhanced preconcentration and selectivity over conventional PDMS as a solid extraction phase. More detailed results will be presented at the conference.

BiographySeung-Woo Lee obtained his PhD degree in Chemistry and Biochemistry from Kyushu University, Japan, in 1999. He is now a Professor of the Graduate School of Environmental Engineering of the University of Kitakyushu, Japan. His current scientific interests include organic/inorganic nanohybrids, molecular imprinting using metal oxide thin films, and GC-MS analysis and chemical sensing of biological compounds.

[email protected]

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August 18-20, 2014




Rodolphe Marie et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Mapping single DNA molecules to the human genome in a nanofluidic deviceRodolphe Marie1, Jonas N Pedersen1, David LV Bauer2, Kristian H Rasmussen1, Mohamed Yusuf2, Emanuela Volpi2, Kalim U Mir2, Henrik Flyvbjerg1 and Anders Kristensen1

1Technical University of Denmark, Denmark2Wellcome Trust Centre for Human Genetics, United Kingdom

Single DNA molecules of genomic length can be stretched by confinement in nanofluidic channels. Nanofluidic devices have been used to characterize the base pair sequence, or the methylation of DNA by imaging fluorescence barcodes

of single molecules. The resolution of the fluorescence barcode imaged on DNA is maximized when the DNA is stretched to its full contour length (0.34 nm per base pair). In nanochannels, DNA stretching is provided by confinement only i.e. DNA can be fully stretched if the channel cross-section matches the persistence length of the DNA (50 nm), which can be challenging to fabricate. A nanofluidic device where 98% stretching of genomic DNA is achieved by an additional mechanism: the hydrodynamic drag of a buffer flow, was designed. At such high stretching, the number of base pairs included in the diffraction limit is minimized thus providing the best barcode resolution obtainable using conventional epifluorescence (about 1 kilobase). A device was used to image fluorescence barcodes of human DNA fragments obtained by proteolysis of metaphase chromosomes. The fluorescence barcodes are specific to the underlying base sequence of each fragment covering a minimum of 1.4 mega base pairs. It has been shown that the barcode image enables to map each fragment to its origin in the human reference genome. Moreover, it was able to detect large structural variations (from a couple of kilobase and up) present in single copies of the human genome by comparing the fluorescence pattern of a given molecule to the pattern expected from the human reference genome (hg18).

BiographyRodolphe Marie has completed his PhD in 2004 from the Technical University of Denmark (DTU) and postdoctoral studies from Lund University in Sweden. He is an Associate Professor at DTU Nanotech, the department of micro and nanotechnology at DTU.

[email protected]

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August 18-20, 2014




Shu-Hua Cheng, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Electrochemical determination of pyrogallol at conducting poly(3,4-ethylenedioxythiophene) film-modified screen-printed carbon electrodesShu-Hua ChengNational Chi Nan University, Taiwan

The electrochemical oxidation of pyrogallol at electrogenerated poly (3,4-ethylenedioxythiophene) (PEDOT) film-modified screen-printed carbon electrode (SPCE) was investigated. The voltammetric peak for the oxidation of pyrogallol in pH

7 buffer solution at the modified electrode occurred at 0.13 V, much lowered than the bare SPCE and preanodized SPCE. All experimental parameters, including the electropolymerization conditions and solution pH values, were optimized to improve voltammetric responses. A linear calibration plot based on flow-injection amperometry was obtained for 1-1000 μM pyrogallol, and a slope of 0.030 μA/μM was obtained. The detection limit (S/N=3) was 0.63 μM.

BiographyShu-Hua Cheng completed her PhD in chemistry from National Taiwan University in 1994. After teaching in Chia Nan University of Pharmacy and Science for five years, she moved to Department of Applied Chemistry at National Chi Nan University, and promoted to full Professor in 2006. Presently her research works mainly focus on the design of conducting polymers and hybrids, and fabrication of modified electrodes for sensitive and selective determination of small molecules related to health care and environmental monitoring.

[email protected]

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August 18-20, 2014




L A Frank, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Coelenterazine-dependent bioluminescent proteins as effective reporters for in vitro assayL A FrankInstitute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Russia

Nowadays, the light-emitting proteins are the promising analytical tool for both in vitro and in vivo applications to meet the growing demands of science and medicine. The great part of analytical techniques is based on coelenterazine-dependent

bioluminescent systems derived from luminous marine organisms. Bioluminescent signal in the organisms arises as a result of coelenterazine (CE) oxidation catalyzed by special enzymes, luciferases. The CE-dependent luciferases of different origin (coelenterates, ctenophores, copepods, ostracods, etc.) known for today are relatively small single-chain proteins having nothing common but a substrate. Luciferases of special type, Ca2+-regulated photoproteins, are stable complexes of apophotoprotein and pre-oxidized substrate molecule - peroxycoelenterazine, which is strongly but non-covalently immobilized in the protein hydrophobic cavity. Bioluminescent reaction is triggered with Ca2+ producing coelenteramide, CO2, and a flash of blue light. Several CE-dependent luciferases are comprehensively studied as to biochemical properties, tertiary structures, bioluminescence mechanism etc.

Application of photoproteins and luciferases as reporters in binding assay has many prospects due to the high quantum yield of bioluminescent reaction, providing high-sensitivity detection; assay robustness, reproducibility, and safety. Of special interest is the use of luciferases genetically modified so to obtain novel enzymes with unique properties such as shifted bioluminescence spectra, varied kinetics, thermostability, or the enzymes fused with polypeptide modules (mini antibodies, biotinylated site, etc.) that are responsible for the assay specificity.

BiographyL A Frank got her PhD degree in Biophysics (1997) and Doctor. of Sciences degree in Biology (2010) at the Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk. At present, she is a leading researcher of the Institute and Professor of the Siberian Federal University. Her research interests are concerned with investigating structure and function of light-emitting proteins and development of analytical systems on their base. She is the author and co-author of more than 100 publications and several international and Russian patents.

[email protected]

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August 18-20, 2014




Doo Soo Chung et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Liquid extraction surface analysis coupled with capillary electrophoresis to determine organophosphorous pesticides on appleDoo Soo Chung, In Hye Sung and Sung Min ChoSeoul National University, Korea

A surface-sampling technique called liquid extraction surface analysis (LESA) was coupled with capillary electrophoresis (CE) to determine organophosphorus pesticides, including glufosinate-ammonium, aminomethylphosphonic acid, and

glyphosate on the external surface of a fruit such as an apple. A solution containing the pesticides was sprayed onto a solid surface. Without any sample pretreatment, the dried analytes on the surface were directly extracted to a hanging drop of extractant at the inlet tip of a capillary. This extraction was made possible by a liquid microjunction that is formed between the sample surface and the extractant drop. The extraction efficiency was enhanced by repeating steps of dispensing and aspirating the extractant drop. After extraction, the analytes were derivatized in-capillary with a fluorophore 4-flouro-7-nitro-2,1,3-benzoxadiazole and analyzed by CE-laser induced fluorescence detection. The limits of detection of the glufosinate-ammonium and glyphosate with LESA-CE were 20-fold lower than the EPA tolerance levels.

BiographyDoo Soo Chung has completed his PhD from Harvard University and postdoctoral studies from MIT and Iowa State University. He has published more than 100 papers in reputed journals.

[email protected]

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August 18-20, 2014




Andrew C Benniston, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Measuring viscosity on the nanoscale using fluorescent molecular rotorsAndrew C BennistonNewcastle University, UK

Molecular environment-sensitive probes offer the opportunity to chart physical and structural alterations on the nanoscale. Many areas of science have benefited from the unique information afforded by probes located within inaccessible spaces,

which could not be collected by conventional techniques. Response to pH, polarity, temperature, extraneous metal ions, poisons and biomolecules are common place. Luminescence has certainly been one of the most popular methods used for readout purposes, since it is highly sensitive and non-intrusive when employed for biological applications. Temporal profiling is also possible with luminescence, so that timescales (e.g., picoseconds to milliseconds) for molecular events is achievable. There are a wealth of fluorescence reporters to date, some of which are tailor-made for specific purposes such as reactive oxygen species (ROS) detection, lipid mobility monitoring, protein sequencing and DNA/RNA recognition. Certainly one of the most versatile classes of fluorescent reporters to date is based on the borondipyrromethene (Bodipy) group. Generally, the fully alkylated molecule (BD) is strongly fluorescent in fluid solution at room temperature. It is very noticeable that fluorescence is much lower for certain fully non-alkylated versions (ROT), especially in non-viscous solvents. There is an enhancement (ca. 4 fold) in fluorescence quantum yield as the solvent viscosity increases by around 10 cP. The solvent viscosity effect is traced to reduction in the non-radiative decay process and the retardation in rotation of the meso aryl group with the increase in solvent viscosity. As the aryl group rotates it distorts slightly the pyrromethene backbone, which in turn affects the rate for non-radiative decay. The one problem with the first prototype of so-called Bodipy molecular rotor was the low starting point fluorescence output. We were especially interested to see if the original signal could be enhanced, with no detrimental effect on the overall fluorescence viscosity response. This talk will discuss our current progress in producing rheological probes (ROFRET) using intramolecular energy transfer to try and enhance the output signal.

BiographyAndrew C Benniston completed his PhD from Warwick University in 1990 and postdoctoral studies at the Universite Louis Pasteur (Strasbourg) and the University of Texas at Austin. He is Professor of Photonic Energy Sciences at Newcastle University. He has published more than 130 papers in major journals and is currently the Editor-In Chief for the Journal of Analytical & Bioanalytical Techniques.

[email protected]

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August 18-20, 2014




S Shtykov et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Supramolecular and nanobiomimetic approach to optimization of analytical reactionsS Shtykov, O Egunova and M Z T Al-SaidiSaratov State University, Russia

The base for this approach is spontaneous and self-assembly formation in the solution of different kinds of liquid nanoreactors (micelles, microemulsions, vesicles, cyclodextrins, calixarenes, LB, L-B-L films) simultaneously with

the analytes and analytical reagents i.e. supramolecular effect. As a result of inclusion of the analytes and reagents into the nanoreactors, the guest microenvironment changes and provokes the change of their protolytic, tautomeric, complexing and a reactivity properties, hydration and analytical characteristics. Several features of liquid nanosystems, which are the base for their biomimetic behavior and enhancement of the analytical and metrological characteristics in chemical analysis and separation, are as follows:

• The ability to concentrate and bring close together the components of analytical reaction in the nanoreactor pseudophase even though they are considerably different in hydrophobicity;

• The multicenter and multifunctional noncovalent interactions of the components of nanophase with solubilized substrate; among these interactions, the hydrophobic ones plays a predominant role;

• The pronounced oriented sorption and cavity effect, in which the nature and geometric compatibility of the host and guest are the decisive factors for the binding of a substrate;

• The considerable microheterogeneity of the medium within nanopseudophase which manifests itself in dramatic changes in the dielectric constant, micropolarity, microacidity, microviscosity and other physicochemical properties of the unique medium with gradient of these properties.

• As a result, dramatic enhancements of all kinds of analytical signals in many methods takes place, and new methods are appeared.

• The work was supported by RFBR, project no. 12-03-00450a.

BiographySergei Shtykov has completed his PhD from Saratov State University (SSU) in 1980 and Doctorate dissertation (Habilitation) from the SSU in 1990. From 1993 he is a Full Professor on the chair of Analytical Chemistry and Chemical Ecology of SSU. From 2005 he is a member of Division of Analytical Chemistry of the European Association for Chemical and Molecular Sciences (DAC EuCheMS). He has published more than 250 papers in academic journals and was Supervisor of 20 PhD and 8 Doctorate dissertations. His scientific direction is an application of nanoobjects, nanotechnologies and supramolecular principles in the chemical analysis.

[email protected]

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August 18-20, 2014




Yoon-Bo Shim et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Simultaneous determination of phthalate esters in a microfluidic device coupled with an electrochemical sensorYoon-Bo Shim, Hui-Bog Noh, Min Young Kim and Dong-Min KimPusan National University, South Korea

An on-chip preconcentration, separation, and electrochemical detection method was developed for the simultaneous determination of endocrine disruptor (ED), phthalate esters using a microfluidic channel device. The electrochemical

analysis of phthalate esters in aqueous media is difficult using conventional methods due to the extremely negative potential for the reduction of them. Thus, the sensor probe layer was assembled with dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) and toluidine blue O (TBO) on the conductive polymer layer to capture and reduce the EDs on the probe through the control of the surface charge and hydrophobic properties. Terthiophene benzoic acid was synthesized and electrochemically polymerized, and then a DOPE and TBO were chemically bonded together on polyTTBA. The modified sensor probe shows the reduction peak of phthalate esters around -1.6 V in 0.1 M buffer solution (pH 10.0), while the bare electrode doesn’t show any redox peak of them. The sensor probe was firstly examined for the electrochemical detection of five phthalate esters using voltammetry and chronoamperometry. Then, the microfluidic channel coupled with the sensor probe was used for the simultaneous analysis of them. Experimental parameters affecting the analytical performance were assessed and optimized in terms of ratio of DOPE:TBO (v/v%), detection potential, pH, and running buffer concentration. The dynamic linear range and detection limits were 0.15 nM - 1.0 µM and 12.5±1.5 pM with relative standard deviations of <5%. The reliability of the proposed method was evaluated with real environmental samples that show excellent performance for the analysis of a phthalate ester family, one of major EDs.

BiographyYoon-Bo Shim has completed his PhD in 1985 from Pusan National University and postdoctoral studies from University of New Mexico (USA). His major is analytical chemistry and electrochemistry. He is a Professor of Chemistry department and the Director of Institute of BioPhysio Sensor Technology at Pusan National University. He has published more than 280 papers in reputed journals and has been serving as editorial board member of Electroanalysis, Sensor, Chemosensor.

[email protected]

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August 18-20, 2014




S Libertino et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Optical Si-based biosensors: First resultsS Libertino3, M F Santangelo1,2, E L Sciuto3, F Sinatra4, S Conoci5, D N Sanfilippo5, G Fallica5, S Lombardo3 and A Busacca2 1Distretto Tecnologico Sicilia Micro e Nano Sistemi, Italy2University of Palermo, Italy3Istituto per la Microelettronica e Microsistemi (CNR-IMM), Italy4Università degli Studi di Catania, Italy5STMicroelectronics, Italy

Optical biosensors based on the use of fluorescent dyes are commonly employed in biomedical applications (e.g. DNA microarray). The optical signal is the transduction mechanism used to recognize DNA hybridization between probes

anchored on a surface and the labelled DNA target. Labelling is performed conjugating optical fluorophores to the target DNA molecule and the detection system is based on optical scanners or CCD cameras. Finally, optical images are elaborated in a post-acquisition analysis through complex softwares. Aim of our work was the fabrication and characterization of optical biosensors using traditional and novel fluorescent dyes and a novel sensor. The fluorophores used are the traditional CY5 and a newer organic molecule, the Ru(bpy)3

2+, while the photodetectors are a pixel array of solid state photon-detectors (Silicon Photomultipliers, SiPM), produced by ST Microelectronics in Catania. These devices have been also employed to study the dyes emission features (lifetimes and emission spectra). Finally, SiPM were used as photon counters to detect the fluorophore signal of dyes coupled to single-strand (ss) or double-strand (ds) DNA. Pulsed measurements performed on Cy5 emission allowed us to conclude that SiPM can be used as photon counter also for biosensing applications. The use of Ru(bpy)3

2+, exhibiting a large difference between the excitation and the emission wavelengths, could allow to implement new detection systems, also enabling different detection parameters, such as the fluorophore lifetime.

BiographyS Libertino got her PhD (1998) at the University of Catania. From 1997 she works at the Microelectronic and Microsystems institute (IMM) of the Italian National Council of Research (CNR), since 2007 with the role of Senior Researcher. Her research interests are oriented to the design and fabrication of Si-based microelectronic and optoelectronic devices and to biological molecules integration in these devices for sensor applications. She has co-authored 3 chapters of books and more than 100 papers published in international journals. She holds 3 European patents, all extended to USA.

[email protected]

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August 18-20, 2014




Yuri A Zolotov, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Some trends in analytical chemistry developmentYuri A ZolotovMoscow State University, Russia

Twelve trends in analytical chemistry are considered, mostly general ones: Movement towards out-of-laboratory, speciation, non-destructive analysis, automatization and miniaturization, pattern recognition, moving towards more active application

of achievements of physics or biochemistry. Directions of the changes in analytical techniques, e.g. their hybridization, will also be discussed. There are changes even in analytical community and approaches to education.

BiographyYury A Zolotov has received his Candidate of Sci. and Dr. of Sci. degrees from Vernadskii Institute of Geochemistry and Analytical Chemistry. He was elected as a full member of the Russian Academy of Sciences and President of Mendeleev Russian Chemical Society. He has published more than 600 papers and about 30 monographs in analytical chemistry and liquid-liquid extraction. He has received many national and international awards and has been invited to give plenary and keynote lectures at many scientific conferences. He is a head of analytical chemistry department of Moscow University.

[email protected]

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August 18-20, 2014




Celina Nazareth et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Development and validation of HPTLC method for simultaneous estimation of olmesartan medoxomil and indapamide in tablet dosage formCelina Nazareth1, B Shivkumar2, Prasad Reddy3 and B M Gurupadayya4

1PES’s Rajaram and Tarabai Bandekar College of Pharmacy, India2BLDEA College of Pharmacy, India 3Samskruti College of Pharmacy, India4JSS University, India

A simple, precise, specific and accurate high performance thin layer chromatographic method has been developed for the simultaneous determination of olmesartan medoxomil and indapamide in pharmaceutical dosage form. The separation was

carried out on Merck HPTLC aluminium plates of silica gel 60F254, using toluene: chloroform: ethanol (4:4:1 v/v) as the mobile phase. HPTLC separation of the two drugs followed by densitometric measurement was carried out in the absorbance mode at 254 nm. The drugs were resolved with Rf values of 0.15 and 0.47 for olmesartan medoxomil and indapamide, respectively. The linear regression analysis data for the calibration plots showed good linear relationship with r value 0.99930 and 0.99660 for olmesartan medoxomil and indapamide respectively, in the concentration range of 100 to 700 ng/spot for olmesartan and 100 to 600 ng/spot for indapamide. The method was validated according to the ICH guidelines with respect to accuracy, precision, specificity and robustness. The limit of detection and quantitation were 100 and 300 ng/spot respectively for olmesartan and 100 and 300 ng/spot for indapamide. The proposed developed HPTLC method can be applied for identification and quantitative determination of olmesartan medoxomil and indapamide in bulk drugs and pharmaceutical dosage form.

[email protected]

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August 18-20, 2014




Wen-Hsi Cheng et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Sampling gaseous compounds of heating essential oil using solid phase microextraction devicesWen-Hsi Cheng and Chin-Hsing LaiFooyin University, Taiwan

This investigation compared the extraction efficiency of needle trap samplers (NTS) with that of the commercial 100 μm polydimethylsiloxane-solid phase microextration (PDMS-SPME) fiber sampler when applied to sample heating products

from tea tree essential oil. The experimental results indicated that NTS performed better effectiveness than those of SPME fiber sampler, and the NTS, packed with 80-100 mesh divinylbenzene (DVB) particles, primarily adsorbed 5.7 ng ethylbenzene, 5.8 ng m/p-xylenes, 11.1 ng 1,2,3-trimethylbenzene, 12.4 ng 1,2,4-trimethylbenzene and 9.99 ng 1,4-diethylbenzene when thermal ceramic wicks were used to evaporate the tea tree essential oil during 1-hr evaporation period.

[email protected]

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August 18-20, 2014




Deia Abd El-Hady et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Using of short chain alkyl imidazolium ionic liquids in enhancing the sensitivity of capillary electrophoresisDeia Abd El-Hady1,2 and Hassan M Albishri21King Abdulaziz University, Saudi Arabia2Assiut University, Egypt

Recently, ionic liquids (ILs) have gained in popularity as unique solvents in different areas of separation techniques. Owing to tunable properties which can be selected by choosing appropriate cationic or anionic constituents, they can be applied

in different separation processes. The current work focuses on the applications of short chain alkyl imidazolium ILs to enhance the sensitivity of capillary electrophoresis (CE) for compounds measurements in complicated matrices. So far the applications of ILs in different modes of CE is still growing and attracting great attention. It seems to be obvious that new possibilities of ILs applications in CE will also be discovered in future.

BiographyDeia Abd El-Hady has completed his PhD from Assiut University, Egypt joined with University of Bologna in Italy. He got postdoctoral grant by DAAD to study at Technical University of Braunschweig, Germany. Currently, he is an associate professor in King Abdulaziz University, Saudi Arabia. He has published more than 30 papers in reputed analytical journals.

[email protected]

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August 18-20, 2014




Gaolin Liang et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Fluorescence switch for selectively sensing copper (II) and L-Histidine in vitro and in living cellsGaolin Liang and Xiaojing WangUniversity of Science and Technology of China, China

Herein, we report the development of a new fluorescence switch for selectively sensing Cu2+ and L-Histidine (L-His) in vitro and in living cells for the first time. In the absence of metal ions, Ac-SAACQ-Gly-Gly-Gly-Lys (FITC) (1) exhibits

comparable fluorescence to that of free FITC. In the presence of metal ions, 1 selectively coordinates with Cu2+, causing its fluorescence emission quenched via photoinduced electron transfer. Interestingly, as-formed 1-Cu2+ complex selectively responds to L-His among the 20 natural amino acids by turning its fluorescence on. This property of fluorescence switch of 1 was successfully applied to qualitatively and quantitatively sensing Cu2+ and L-His in vitro. Using this dual functional probe, we also sequentially imaged Cu2+ and L-His in living HepG2 cells. The new probe 1 could be applied for not only environment monitoring or biomolecule detections, but also disease diagnoses in the near future.

BiographyGaolin Liang is full Professor at University of Science and Technology of China since March 2010. He received his BS from Nanjing University in 1993, MS from Zhengzhou University in 2002, and PhD from Fudan University in 2005. From 2005 to 2008, he was a postdoctoral fellow at The Hong Kong University of Science and Technology under the supervision of Professor Bing Xu. From 2008 to 2010, he was a postdoctoral fellow at Stanford University under the supervision of Professor Jianghong Rao. His research interests mainly focus on nanochemistry, molecular and cellular imaging, and biomedical analysis.

[email protected]

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August 18-20, 2014




Xin Chen, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

An in situ liquid cell TEM study on nanomaterial depositions and nano characterizationsXin ChenEast China University of Science and Technology, China

In situ liquid cell TEM technology has been used to study nano material deposition by means of liquid phase electron beam induced deposition (LP-EBID). The electron beam energy varies from 100 keV to 200 keV. Firstly, using a HAuCl4 solution

as the precursor and under a broad electron beam, scattered gold nano particles of 14-72 nm have been deposited. Then using SiCl4 and SiCl4 in CH2Cl2 solutions as the precursors and under well focused electron beam irradiations, localized Si and SiCx nano dots and nano wires have been developed. The size and shape of the Si and Six nano structures can be well controlled by adjusting the deposition parameters, and the nanostructures are found to attach well to the Si3N4 window substrates of the liquid cell, showing good promise for future nanoelectronic device developments. Besides these inorganic nanostructures, the in situ TEM technology has been further successfully used to perform nano characterizations on organic composites and biological cells.

BiographyXin Chen has completed his PhD from University of Houston and Postdoctoral studies from University of Houston. He served as Visiting Research Assistant Professor in University of Illinois at Urbana-Champaign, and he is now Shanghai Thousand Plan Professor in East China University of Science and Technology. He has published more than 30 papers in reputed journals, made invited talks in several international conferences, and edited a book. He is a referee of over 15 reputed journals, and he has served as committee member in several reputed research societies and international conferences.

[email protected]

Page 55



August 18-20, 2014




Huangxian Ju, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Design of nanoprobes for in situ analysis of cellular functional biomoleculesHuangxian JuNanjing University, China

Cellular functional biomolecules, such as glycans, microRNA, telomerase etc., regulate a variety of vital biological events, and thus have been regarded as attractive targets for biomedical research, molecular diagnostics and disease therapy. Due

to the lack of noninvasive methods for interrogation of the related biological process, our recent efforts have been devoted to the fabrication of nanoprobes for in situ analysis of various cellular functional biomolecules and highly selective photodynamic therapy (PDT). For cell surface glycan detection, we proposed a novel competitive recognition format and a solution encoding strategy for quantitative analysis of multiple glycans on living cells, and fabricated a disposable electrochemical cytosensor array for simultaneous monitoring of multiple glycans on intact cell surfaces. Focusing on the urgent need for in situ analysis of intracellular telomerase, we designed a mesoporous silica nanoprobe and a nicked molecular beacon by using telomerase-responsive primer DNA to produce “off-on” imaging of intracellular telomerase activity. In order to monitor intracellular miRNA levels, a polyethylenimine-grafted graphene nanoribbon and a multifunctional SnO2 nanoprobe were fabricated for simultaneous specific delivery, cell imaging and intracellular miRNA detection. The SnO2 nanoprobe could also be used for regulating the expression of intracellular miRNA level. To achieve highly selective PDT, we proposed two cell-specific and acidic pH-activatable nanoparticles by introducing selenium into rubyrin core to enhance the 1O2 generation efficiency and dimethylaminophenyl moiety at mesoposition of rubyrin to achieve pH-controllable activity, and using R16FP to generate 1O2 and BDP-688 for therapeutic monitoring, which combined folate and selected aptamer at the surface of nanoparticles to obtain cancer cell targeting feature, respectively. We believe these studies would accelerate the understanding of biological roles of these functional biomolecules and provide valuable tools for in vivo clinical research.

BiographyHuangxian Ju received BS, MS and PhD from Nanjing University during 1982-1992 and was a postdoc in Montreal University from 1996-1997. He became an associate and full professor of Nanjing University in 1993 and 1999. He won the National Funds for National Distinguished Young Scholars in 2003 and National Creative Research Groups in 2006. He was selected as a Changjiang Professor in 2007, a Chief Scientist of National Basic Research Program of China in 2009 and the Director of State Key Laboratory of Analytical Chemistry for Life Science in 2011. His research interests focus on analytical biochemistry and molecular diagnosis. He has published 457 papers with an h-index of 60 and SCI citation of 12175, authored 27 patents, 2 English books, 6 Chinese books, and 6 Chinese and 8 English chapters.

[email protected]




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Analytica Acta-2014


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Analytica Acta-2014

Day 2 August 19, 2014

3: Novel Approaches to Analytical & Bioanalytical Methods

Track 3

Session ChairMitsumasa IwamotoTokyo Institute of Technology, Japan

Session Co-ChairYuewu XiaoMerck Millipore, USA

Title: Utilizing confocal Raman spectroscopy to understand monoclonal antibody purification by a strong cation exchangerYuewu Xiao, Merck Millipore, USA

Title: Identification of CTP-499 metabolites in human plasma and urineChangfu Cheng, Concert Pharmaceuticals, Inc., USA

Title: A vinblastine fluorescent probe for pregnane X receptor in a time-resolved fluorescence resonance energy transfer assayWenwei Lin, St. Jude Children’s Research Hospital, USA

Title: Multifunctional SERS active silver-hybrid nanocomposites in (bio)analyticsInez M Weidinger, Technical University of Berlin, Berlin

Title: Visualization of carrier motion and dielectric polarization in organic thin layers by Optical second harmonic generation measurement and Maxwell-displacement current measurementMitsumasa Iwamoto, Tokyo Institute of Technology, Japan

Title: Evaluation of affinity capillary electrophoresis for ligand binding assaysDeia Abd El-Hady, King Abdulaziz University, Saudi Arabia

Title: Aptamer-functionalized nanoporous gold for directly electrochemical detection of bisphenol AYe Zhu, Shandong University, China

Title: Raman Mapping-A powerful analytical tool for detection/quantification of API physical form and its form impurities as presented in solid samples to support R&D, QbD, license application and fighting against counterfeit medicine products and patent infringementJianping Wu, Pharmaterials, Ltd., UK

Title: Immobilization of DAAO on magnetic nanoparticles modified by reactive polymer for screening enzyme inhibitors with chiral ligand exchange capillary electrophoresis methodLi Qi, Chinese Academy of Sciences, China


Page 63



August 18-20, 2014




Yuewu Xiao, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Utilizing confocal Raman spectroscopy to understand monoclonal antibody purification by a strong cation exchangerYuewu XiaoMerck Millipore, USA

Monoclonal antibody (mAb) purification usually employs a template process, which often includes cation exchange chromatography after the Protein A step. The mAb eluent from the Protein A column is at pH ~3-4, and should be

adjusted to have the optimal pH (~4-5) and conductivity (~2-100 mS/cm) for the subsequent cation exchange chromatography. To obtain the optimized conditions for a mAb and a cation exchanger, the most common trial and error approach is to analyze the loading and elution profiles of the mAb in solution state. In this presentation, a monoclonal antibody (Merck Millipore mAb04) was loaded onto a strong cation exchanger (Fractogel EMD SO3 (M)) at pH 5 or 4 with a conductivity of 10 mS/cm or 2 mS/cm. Confocal Raman spectroscopy was applied, for the first time, to measure protein distribution and conformational changes in chromatographic particles. Both depend on the loading pH and conductivity. This approach therefore can help the researcher to understand, on a very small scale using a single chromatography bead, how and why the loading conditions will affect mAb purification by the cation exchanger at full scale.

[email protected]

Page 64



August 18-20, 2014




Changfu Cheng, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Identification of CTP-499 metabolites in human plasma and urineChangfu ChengConcert Pharmaceuticals, Inc., USA

CTP-499 is a novel oral multi-subtype selective inhibitor of PDEs that is currently in clinical testing, in combination with angiotensin modulators, as a potentially first-in-class treatment for diabetic kidney disease. The compound was

discovered and developed by using Concert’s proprietary DCE Platform® in which deuterium was incorporated at select positions of 1-((S)-5-hydroxyhexyl)-3,7-dimethylxanthine (HDX). The hydroxyhexyl side chain of CTP-499 is extensively metabolized in vivo. Metabolites of CTP-499 in human plasma and urine were identified by HPLC-UV-MSn methods. Five major metabolites, designated as M1, M2, M3, M4, and M5, were observed from pooled human plasma samples. The most abundant metabolite is M5, followed by M1 and M2. Metabolites M3 and M4 were lower in concentration than the others. M1 is formed from the oxidation of the CTP-499 hydroxyl group to a ketone. M2 and M3 are isomeric diols, formed by oxidation of carbon adjacent to the CTP-499 hydroxyl group. M4 and M5 are carboxylic acids which may be produced by a combination of transformations, including oxidations and carbon-chain shortening reactions. The predominant metabolite in human urine is M5. Low concentration levels of M4 and the glucuronide of CTP-499 were also detected in human urine, together with trace levels of CTP-499, M1, M2 and M3.

BiographyChangfu Cheng obtained his Master’s degree in Organic Synthesis at Southern Illinois University in 1994 and completed his PhD training in mass spectrometry at Washington University in St. Louis in 1998. He has worked in the fields of drug metabolism and bioanalysis for more than 15 years. He is currently the Director of bioanalytics at Concert Pharmaceuticals, in charge of the company’s analytical laboratory that supports medicinal chemistry, pharmacokinetics, formulation, stability, structural elucidation and metabolite identification. Because of the company’s unique platform, his work involves many therapeutic areas such as fibrosis, oncology, nephrology, endocrinology and ophthalmology.

[email protected]

Page 65



August 18-20, 2014




Wenwei Lin et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

A vinblastine fluorescent probe for pregnane X receptor in a time-resolved fluorescence resonance energy transfer assayWenwei Lin and Taosheng ChenSt. Jude Children’s Research Hospital, USA

The pregnane X receptor (PXR) regulates the metabolism and excretion of xenobiotics and endobiotics by regulating the expression of drug-metabolizing enzymes and transporters. The unique structure of PXR allows the binding of many

drugs and drug leads to it, possibly causing undesired drug-drug interactions. Therefore, it is crucial to evaluate whether lead compounds bind to PXR. Fluorescence-based assays are preferred because of their sensitivity and nonradioactive nature. One fluorescent PXR probe is currently commercially available; however, because its chemical structure is not publicly disclosed, it is not optimal for studying ligand-PXR interactions. Here we report the characterization of BODIPY FL-vinblastine, as a high-affinity ligand for human PXR with a Kd value of 673 nM. We provide evidence that BODIPY FL-vinblastine is a unique chemical entity different from either vinblastine or the fluorophore BODIPY FL in its function as a high-affinity human PXR ligand. We describe a BODIPY FL-vinblastine-based human PXR time-resolved fluorescence resonance energy transfer assay, which was used to successfully test a panel of human PXR ligands. The BODIPY FL-vinblastine-based biochemical assay is suitable for high-throughput screening to evaluate whether lead compounds bind to PXR.

BiographyWenwei Lin has completed his PhD at the University of Tennessee, Health Science Center and postdoctoral studies at the St. Jude Children’s Research Hospital. He is currently a high throughput screening specialist in the High Throughput Screening Center, St. Jude Children’s Research Hospital. He has authored or co-authored more than 20 papers in reputable journals.

[email protected]

Page 66



August 18-20, 2014




Inez M Weidinger et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Multifunctional SERS active silver-hybrid nanocomposites in (bio) analyticsInez M Weidinger1, Xiao Xia Han1,2, Murat Sezera, Arumugam Sivanesan1,3 and Peter Hildebrandt11Technische Universität Berlin, Germany2Jilin University, China3RWTH Aachen University, Germany

Surface enhanced resonance Raman spectroscopy (SERRS) can be used in analytical science to identify target molecules at very low concentrations. The combination of this method with other techniques such as magnetic manipulation or

electrochemistry allows constructing new analytical devices that exhibit several functionalities. In the talk, the author will show several examples of such multifunctional SERRS Hybrid devices. In the first example the use of Ag@SiO2 and Ag@chitosan nanoparticles for selective detection and separation of target proteins in a mixed sample is demonstrated. In the second example Fe3O4@Ag-Myoglobin nanoparticles are presented that are capable of detecting and magnetically removing small toxic molecules in water. Fe3O4@TiO2-Ag nanoparticles are used in the third example to detect, collect and catalytically decompose toxic pollutants. Here also the positive effect of plasmonic Ag nanoparticles on photocatalytic degradation is discussed. The last example demonstrates how SERRS spectro-electrochemistry is able to get insight into the functionality of enzymatic biosensors.

[email protected]

Page 67



August 18-20, 2014




Mitsumasa Iwamoto, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Visualization of carrier motion and dielectric polarization in organic thin layers by optical second harmonic generation measurement and Maxwell-displacement current measurementMitsumasa IwamotoTokyo Institute of Technology, Japan

Optical second harmonic generation (SHG) measurement has been widely used to characterize the structure of organic thin films, including Langmuir films on the water surface, where non-linear optical susceptibilities of organic films which

originates from arrangement of molecules and nonlinear molecular susceptibility are well probed. However, once we focus on the electric field coming out from electron motion and rotational dipolar motion, which are governed by the Gauss law, we can directly probe electron motion in organic thin films as well as rotational dipolar motion in monolayer. The technique we use here is a novel optical second harmonic generation technique based on electric field induced optical second harmonic generation (EFISHG) measurement. Using EFISHG, we visualize carrier motions in organic thin films, which are introduced in organic devices. In this presentation, we first discuss basic principle of EFISHG for probing dynamical carrier motion in organic films, and then visualize carrier motion. Similarly we show rotational dipolar motion in organic monolayer is directly probed by using Maxwell-displacement current. Finally as the extension of SHG measurement, we show the advanced conventional optical second harmonic generation measurement coupled with the Brewster Angle Microscope (BAM), which is capable of visualizing polarization structure of Langmuir-monolayers. We then demonstrate that the domain shapes of mixed lipid layers which are visualized by SHG and BAM are the same, but we can see textures originated from induced dipoles, different from macroscopic domain patters. Finally we show rotational dipolar motion in organic layer is directly probed by using Maxwell-displacement current.

BiographyMitsumasa Iwamoto completed his PhD at Tokyo Institute of Technology in 1981, and now a Professor of the same university. He is a fellow of JSAP (Japan Society of Applied Physics), and also a fellow of IEICE (The Institute of Electronics, Information and Communication Engineers). He is a council member of Association of Asia Pacific Physical Societies (AAPPS). He has published many papers in reputed journals, such as Nature, Nature photonics, Physical Review Letters, etc., and he wrote and edited many books, such as “The physical Properties of Organic Monolayers” (World Scientific, Singapore, 2001) and “Nanoscale-interface for organic electronics”.

[email protected]

Page 68



August 18-20, 2014




Deia Abd El-Hady et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Evaluation of affinity capillary electrophoresis for ligand binding assaysDeia Abd El-Hady1,2, Hassan M Albishri2 and Hermann Watzig3

1King Abdulaziz University, Saudi Arabia2Assiut University, Egypt3Institute of Medicinal and Pharmaceutical Chemistry, Germany

Process analytical technologies need precise and fast analytical tools especially in the early evaluation stage of drug discovery. Here, in the evaluation of ligand (drug) and receptor (plasma protein), affinity capillary electrophoresis (ACE) offers a

lot of advantages including high separation efficiency, low sample volume, using of impure samples, ease and low cost of automation and ability to work under native physiological conditions. Furthermore, our recent published works suggested reduced experimental designs in which a single experiment could suffice. Then, the realistic duration of one binding-constant determination, including analysis and rinsing times, does not need to be more than a couple of hours, and this speed could be further multiplied by capillary arrays or miniaturized systems. Therefore, various analytical aspects of ACE including method development, and instrument qualification will be discussed. Precision certainly is a major parameter, to comply with acceptance criteria in quality control (QC), but also to investigate changes in biological systems. Further robustness, accuracy and selectivity are considered. In the case of high binding constants of the order of 105 per mol or higher, ACE is not applicable in its commonly used form. However, an advanced ACE version can also reliably detect high binding constants, which will be highlighted as an important feature.

BiographyDeia Abd El-Hady has completed his PhD at the age of 32 years from Assiut University joined with University of Bologna in Italy. He got postdoctoral grant by DAAD to study at Technical University of Braunschweig, Germany. Currently, he is an Associate Professor in King Abdulaziz University, Saudi Arabia. He has published more than 30 papers in reputed journals.

[email protected]

Page 69



August 18-20, 2014




Ye Zhu, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Aptamer-functionalized nanoporous gold for directly electrochemical detection of bisphenol AYe ZhuShandong University, China

In the present work, a highly sensitive and selective biosensor for directly electrochemical detection of bisphenol A was developed based on aptamer-functionalized nanoporous gold. Nanoporous gold was prepared by dealloying Ag from Au/Ag

alloy leaves in concentrated nitric acid. Nanoporous gold was attached onto glassy carbon electrode and then functionalized with bisphenol A-specific aptamer via the formation of Au-S bond. The morphology of fabricated sensor was studied by SEM and the immobilization of aptamer was demonstrated by XPS. Nanoporous gold exhibited excellent catalytic activity toward the redox reaction of BPA captured by aptamer, which led to ultrasensitivity. Moreover, the aptamer immobilized on nanoporous gold improved the selectivity of the developed biosensor. The experimental parameters in terms of aptamer concentration, reaction time, pH, and temperature were optimized. The calibration curve was constructed and the detection limit was determined to be at subfemtomole. The developed biosensor showed promising potential in real sample analysis.

BiographyYe Zhu is currently a lecturer in the School of Chemistry and Chemical Engineering at Shandong University, China. She received her BE and MSc from Chongqing University, China. She was awarded her PhD in 2012 from Pusan National University, South Korea. She has published several papers in reputed journals. Her current research interest is mainly focused on the development of electrochemical biosensors for the detection of environmental pollutants and disease biomarkers.

[email protected]

Page 70



August 18-20, 2014




Jianping Wu et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Raman mapping-A powerful analytical tool for detection/quantification of API physical form and its form impurities as presented in solid samples to support R&D, QbD, license application and fighting against counterfeit medicine products and patent infringementJianping Wu and Shahid RazaPharmaterials, Ltd., UK

The regular analytical techniques for solid materials, such as XRPD, macro spectroscopy, thermal and/or gravimetric analyses, cannot provide ‘micro-scale’ information such as API crystal size, agglomeration, distribution, which could be

vital for the initial formulation development, QbD, and final commercial product development. In addition, the aforementioned techniques generally have poor sensitivity to detect sub-percent (<1% w/w) API and/or its form impurities as presented in a solid sample. A technique with such capability could support QbD and license application for generic products to regulatory authorities. The identification of the patented physical form of API as presented in the tested solid sample, generally at a small amount, can provide solid evidence against counterfeit medicine products and patent infringement. Raman mapping is one of the most promising techniques to fulfil the aforementioned tasks. The importance of Raman mapping is largely due to the capability of simultaneously obtaining a great amount of Raman spectral and spatial information from a solid pharmaceutical or biopharmaceutical sample. However, there are some practical issues that hinder the application of Raman mapping to pharmaceutical and biopharmaceutical industry. In this presentation, the authors show the capability of Raman mapping through practical examples how to overcome these issues. This presentation presents its applications in developing single phase solid dispersion formulations; supporting license application with capability of detection/quantification of API and/or its form impurities at sub-percent (<1% w/w) in some potential commercial products and analyses of counterfeit products in a couple of legal casework.

BiographyJianping Wu has completed his BSc and MEng in China and PhD in the UK. He started his career in pharmaceutical industry from Pfizer (Sandwich, UK) by using Raman microscope. He is now an expert with respect to pharmaceutical R&D from early stage API form selection and initial formulation developments for clinical studies to GMP manufacturing of commercial products. He acted as expert assistant and has taken part in more than 10 legal caseworks. He is now principal Scientist at Pharmaterials.

[email protected]

Page 71



August 18-20, 2014




Li Qi et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Immobilization of DAAO on magnetic nanoparticles modified by reactive polymer for screening enzyme inhibitors with chiral ligand exchange capillary electrophoresis methodLi Qi, Xiao-Yu Mu, Bing-Bing Sun, Yuan Su and Juan QiaoChinese Academy of Sciences, China

Herein, D-amino-acid oxidase (DAAO) has been immobilized on the magnetic nanoparticles, which were modified by biocompatible reactive polymer, poly (glycidyl methacrylate) (PGMA) using atom transfer radical polymerization

technique. It has been found that the enzyme immobilization process could be promoted greatly with the assistance of the catalyst lithium perchlorate. Meanwhile, a new amino acid ionic liquid (AAIL) was successfully synthesized and developed as the efficient chiral ligand in chiral ligand exchange capillary electrophoresis (CLE-CE) system for chiral separation of Dns-D,L-AAs and quantitation of the substrate methionine. Then the apparent Michaelis-Menten constants of the enzyme reactor were determined using the proposed CLE-CE method. The synthesized DAAO@PGMA@Fe3O4 nanoparticles exhibited excellent reusability and good stability. Moreover, the enzyme reactor was successfully applied in screening the DAAO inhibitors. The results demonstrated that the enzyme could be efficiently immobilized on the polymer-grafted magnetic nanoparticles and the obtained enzyme reactor has great potential in screening inhibitors, further offering a new insight for monitoring the relevant diseases.

BiographyLi Qi obtained her PhD degree in Analytical Chemistry at Hebei University, P. R. China. She is an Associate Professor at Institute of Chemistry, Chinese Academy of Sciences and has published 96 scientific papers in reputed journals, such as Analytical Chemistry, Chemical Communications, Journal of Materials Chemistry, Biosensors & Bioelectronics, Chemistry-A European Journal, Journal of Chromatography A, Electrophoresis. Her research interests are capillary/chip electrophoresis, chiral separation, enzyme kinetics, preparation of polymer monolith and its application.

[email protected]

Page 73

Analytica Acta-2014

Track 4, 5 & 6Day 2 August 19, 2014

4: Analytical Techniques in Pharmacogenomics5: NMR & Analysis of Small Organic Molecules6: Advances in Chromatography and Mass SpectrometrySession ChairKarol JackowskiUniversity of Warsaw, Poland

Session Co-ChairSangeeta TannaDe Montfort University, UK

Title: NMR analysis of chemical compounds in the gas phaseKarol Jackowski, University of Warsaw, Poland

Title: Adherence to medication assessed using dried blood spot analysisSangeeta Tanna, De Montfort University, UK

Title: Spectrofluorimetry: A critical review on factors that influence fluorescent intensity in the analysis of diverse chemical substancesAhmad Uba, Usmanu Danfodiyo University, Nigeria

Title: Determination of phosphatidylserine in milk based nutritional products using online derivatization HPLCQi Lin, Abbott Nutrition R&D, Singapore

Title: Analysis of small molecules in vivo by MALDI-TOF MSZongxiu Nie, Chinese Academy of Sciences, China

Title: Extraction and analysis of fatty acids from cyanobacteria using GC x GC-TOFMSTitus A M Msagati, University of Johannesburg, South Africa


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August 18-20, 2014




Karol Jackowski, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

NMR analysis of chemical compounds in the gas phaseKarol JackowskiUniversity of Warsaw, Poland

Nuclear magnetic resonance (NMR) is extensively used for the analysis of chemical compounds. NMR gives quick and precise answer whenever the spectra of unknown material are compared with those of pure chemical compounds.

However the comparison must be always performed maintaining the same experimental conditions otherwise the results of analysis may be not satisfactory, mostly due to different intermolecular interactions. The above problem can be solved if the effects of intermolecular interactions are completely removed from NMR spectra. It is possible when the observation of spectral parameters is performed in the gas phase and the results of measurements are extrapolated to the zero-density limit. At present such experiments can be easily completed also for medium-sized molecules when any inner gas is used as the solvent. In our laboratory we have already performed hundreds of such analyses and we have obtained the NMR parameters of isolated molecules which are doubtlessly the best standards for future applications. We have also proposed the direct shielding measurement with the use of a standard NMR spectrometer. According this method the shielding parameters can completely replace the chemical shifts of numerous nuclei. The new method has also many additional advantages; as it allows for example the direct comparison of experimental and theoretical shielding constants and makes possible the determination of the first order isotope effect in shielding, which was not available in NMR spectroscopy before.

BiographyKarol Jackowski received his PhD in 1974. Since that time he is permanently employed at the University of Warsaw. He was a Visiting Assistant Professor at the University of Illinois at Chicago Circle, USA, for 2 years in 1983-1985 and a Visiting Professor at the University of Western Sydney, Australia in 2011. Presently, he is a Full Professor of Physical Chemistry and NMR Laboratory Head. He has published more than 100 papers in reputed journals and has been serving as an editorial board member of the International Journal of Spectroscopy and the American Journal of Physical Chemistry.

[email protected]

Page 75



August 18-20, 2014




Sangeeta Tanna et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Adherence to medication assessed using dried blood spot analysisSangeeta Tanna and Graham LawsonDe Montfort University, UK

Patients suffering from chronic diseases will gain maximum benefit from oral therapy treatment only if they take their medication(s) as prescribed. Research suggests that up to ~60% of patients are currently prescribed either cardiovascular

drugs or oral chemotherapy drugs, for example patients with breast cancer do not take their medication correctly. This high incidence of non-adherence has direct impact on patient health leading to complications and hospital re-admissions with consequent additional healthcare costs. In the US and UK the health service providers may be fined for re-admissions within 30 days of discharge. This study details checks on adherence to prescription medication using a single drop of blood, from a finger prick, collected on a card and allowed to dry. Liquid chromatography-high resolution mass spectrometry (LC-HRMS) method was used for the determination of candidate drugs in the blood spot to confirm adherence to the prescription. Trials on 6 commonly UK used cardiovascular drugs are reported demonstrating the ability of the system to detect the target analytes during the 24 hour repeat prescription cycle. Samples from volunteers with confirmed adherence were used to validate the response from the system as were samples from volunteers receiving no medication.

No false positives were observed and adherence assessment for Bisoprolol, Ramipril, Amolodipine, Valsartan, Doxasozin and Simvastatin was demonstrated. Furthermore, examples of incorrect adherence were identified.

BiographySangeeta Tanna is a Reader in Pharmaceutical Bioanalysis in the Leicester School of Pharmacy at De Montfort University. Her expertise and research interests lie in the bioanalysis and drug delivery fields. She has developed micro-analytical methodologies for the determination of therapeutic drugs from dried blood spots (DBS) based on LC-MS and LC-MS/MS studies for a range of clinical applications. This research was awarded the Royal Society of Chemistry Analytical Methods Prize in 2010. Demonstrated applications of this work to improve patient care include better medication for babies and the ability to identify non-adherent patients with cardiovascular diseases.

[email protected]

Page 76



August 18-20, 2014




Ahmad Uba, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Spectrofluorimetry: A critical review on factors that influence fluorescent intensity in the analysis of diverse chemical substancesAhmad UbaUsmanu Danfodiyo University, Nigeria

Spectrofluorimetry or simply Fluorometry is a technique which uses the measurement of the intensity of fluorescent light emitted by substance to be examined in relation to that emitted by a given substance. It is a widely used photoluminescence

method in analytical chemistry and is ideal for analysis of small quantities (in microgram) of fluorescent materials particularly where interference in UV-vis spectrometry is a major analytical constrain. It has high sensitivity, selectivity and is most preferred spectrometric assay method for a number of chemical substances including food, and drugs. The success of quantification and its reliability using the method rely solely on the intensity of the fluorescent light emitted by the sample solution and detected at the detector. In this article, critical review of the factors influencing fluorescent intensity including quantum yield, intensity of incident light, path length, adsorption, oxygen, pH, photodecomposition, temperature, viscosity, scatter and quenchers were attempted.

BiographyAhmad Uba has BSc, MSc and PhD, (2010) all in Applied Chemistry from Usmanu Danfodiyo University, Sokoto, Nigeria. He is presently a senior lecturer and Head, Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Usmanu Danfodiyo University, Sokoto. He has more than 20 publications in repute local and international journals and has supervised 74 undergraduates and 10 postgraduate academic works in applied chemistry.

[email protected]

Page 77



August 18-20, 2014




Qi Lin et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Determination of phosphatidylserine in milk based nutritional products using online derivatization HPLCQi Lin, Jie Zhang, Weijie Pei and Chunyan ZhangAbbott Nutrition, Singapore

Phoshpatidylserine (PS) has received interest for its benefit in improving cognitive abilities and behaviors. A new method for determining PS in milk based nutritional products has been developed. The method requires a quick and simple

sample preparation procedure, followed by the high performance liquid chromatography (HPLC) fluorescence detection (FLD) with an on-line FMOC ([(9-Fluorenylmethyl) oxy] carbonyl) derivatization. The method allows PS to be determined in raw materials, milk powder and liquid milk products. The day-to-day (n=3 days) average recovery of over spike-in at 100% fortification level was 100%, and the method quantification limit is at a level of 40 mg per Kg milk powder.

BiographyQi Lin obtained her PhD from University of Wisconsin - Madison. She is a senior analytical scientist at Abbott Nutrition R&D specialized in the compositional analysis of nutritional products. She develops methods for testing of functional ingredients in milk based nutritional products. She has 4 years of experience in analysis of ingredients and additives in various food matrices.

[email protected]

Page 78



August 18-20, 2014




Zongxiu Nie, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Analysis of small molecules in vivo by MALDI-TOF MSZongxiu NieChinese Academy of Sciences, China

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) has been extensively used in the analysis of large molecules, such as biomolecules and synthetic polymers, since the late 1980s. Over the years, MALDI MS has

been an indispensible tool in proteomics, genomics, metabolomics, glycomics, lipidomics, MS imaging, etc. However, small molecules (m/z <1000) analysis is limited in MALDI applications because of the background interference of conventional small organic matrixes in the low mass range. The present study aims to develop novel interference-free matrixes for the analysis of small molecules in biological samples, as well as the living biosystem. Some examples, including (1) high salt-tolerant, and low background N-(1-naphthyl) ethylenediamine dihydrochloride (NEDC), was applied as a matrix to measure the level of glucose in rat brainmicrodialysates by MALDI-TOF MS in combination with in vivo microdialysis will be shown. By monitoring the ion signals of [glucose+Cl]- in the mass spectra, a low detection limit of 10 μM for glucose in 126 mM NaCl, which is a typical component in artificial cerebrospinal fluid, without prior sample purification was achieved; (2) N-(1-naphthyl) ethylenediamine dinitrate (NEDN), was employed as a matrix to analyze small molecules such as oligosaccharides, peptides, metabolites and explosives using negative ion MALDI-TOF MS. For saccharides, the 500 amol LOD was achieved. This matrix was applies in the structural identification of oligosaccharides with post-source decay MALDI-MS; (3) 1-naphthylhydrazine hydrochloride (NHHC) has been selected as an ideal matrix to detect small molecules. This salt-tolerant matrix could be applied for the high sensitive glucose analysis with an ultra-low limit of detection of 1 amol. With NHHC, glucose in serum and the biomarker homogentisic acid in urine were successfully determined by MALDI-TOF MS in negative ion mode; (4) 2,3,4,5-tetra(3’,4’-dihydroxylphenyl)thiophene (DHPT), was designed and synthesized as the MALDI matrix for the selectively analysis of small molecular amines in positive ion mode. A wide range of small amines, such as β-agonists, amino acids, peptides, alkaloid, vitamine B and aromatic amines was analyzed and the low picomole limit-of-detection was obtained. DHPT was also applied for the qualitative analysis of the amine metabolites and quantitative determination of creatinine in urine; (5) Carbon nanodots was applied as a new MALDI matrix in both positive- and negative-ion modes. A wide range of small molecules including amino acids, peptides, fatty acids, as well as β-agonists and neutral oligosaccharides were analyzed. The glucose and uric acid in real samples were quantitatively determined by the internal standard method.

BiographyZongxiu Nie has completed his PhD from Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences and postdoctoral studies from Institute of Atomic and Molecular Sciences, Academia Sinica and Department of Chemistry, Purdue University. He is the professor of chemistry in Institute of Chemistry, Chinese Academy of Sciences. He has published more than 42 papers in reputed journals of Mass Spectrometry.

[email protected]

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Titus AM Msagati et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Extraction and analysis of fatty acids from Cyanobacteria using GC x GC-TOFMSTitus A M Msagati1, Kessy F Kilulya1,2 and Bhekie B Mamba1

1University of Johannesburg, South Africa2University of Dar es Salaam, Tanzania

Cyanobacteria grow in freshwater bodies when they are provided with suitable growth conditions such as nutrients, temperature and light. Algae biomass is known to contain a large amount of lipids such as saturated and unsaturated

fatty acids. In this study fatty acids from algal cells were extracted using a newly developed extraction protocol using ionic liquid enhanced by direct transesterification at an elevated temperature. The identification and quantification of fatty acids was performed using gas chromatography coupled to a time-of-flight mass spectrometer (GCxGC-TOFMS). The extracted fatty acids were dominated by those with carbon chain of C16 and C18; [ie. 7-hexadecenoic acid (C16:1) and hexadecanoic acid (C16:0) for C16, whereas C18 includes γ-linolenic acid (γ-C18:3); linoleic acid (C18:2); linolenic acid (C18:3); 6,9,12,15-octadecatetraenoic acid (C18:4); oleic acid (C18:1) and octadecanoic acid (C18:0)]. The obtained fatty acids composition was then compared with that obtained by organic solvent extraction using a mixture of chloroform and methanol. Statistical evaluation was performed using one-way ANOVA and found that there was no statistically significant difference (P = 0.908) between the two extraction methods, a finding which indicates the usefulness of ionic liquid as a solvent to replace volatile organic solvent to minimize environmental pollution.

BiographyTitus AM Msagati has completed his PhD in 2005 from the University of Botswana and a short postdoctoral studies from Lund University in Sweden. He is currently working at the University of Johannesburg, in South Africa as an associate professor of chemistry (Department of Applied Chemistry). He has published more than 50 papers in reputed journals.

[email protected]




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Analytica Acta-2014


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Analytica Acta-2014

Track 7, 9, 10, 11 & 12Day 3 August 20, 2014

7: Analytical Techniques in Immuno Chemistry 9: Applications of Analytical and Bioanalytical Methods10: Diagnostic Assays and Test Kits11: New Instrumentation and Equipment12: Regulatory Issues and Biosafety Challenges in BioanalysisSession ChairMing LiBiogen Idec, USA

Session Co-ChairZhenxin WangChinese Academy of Sciences, China

Title: Novel phenothiazine enhancers in chemiluminescent enzyme immunoassayIvan Yu Sakharov, Lomonosov Moscow State University, Russia

Title: Automated small molecule bioanalytical sample preparation method developmentMing Li, Biogen Idec, USA

Title: Biosensor for detection of organophosphate pesticide residues by screen printed carbon electrode (SPCE)-chitosan baseAni Mulyasuryani, University of Brawijaya, Indonesia

Title: Development of bioanalytical methods for environmental monitoring of toxic chemicalsYalavarthy Prameela Devi, Kakatiya University, India

Title: Simple and fast analyses using GC-FID for detection of a potential genotoxins (isopropyl para-toluenesulfonate) in palm oil based esters a common ingredient used in cosmetic and personal care productsBonnie Yen Ping Tay, Malaysian Palm Oil Board, Malaysia

Title: Air quality challenges in Wesselton township, South AfricaShadung Moja, University of South Africa, South Africa

Title: Food analysis to check quality, safety and authenticity by full-automated 1H-NMRWenxin (Shirley) Xu, Bruker BioSpin GmbH, China

Title: Bioanalytical applications of gold nanoparticle probesZhenxin Wang, Chinese Academy of Sciences, China

Title: A crystalline self-assembly from nanords: SuperparticlesTie Wang, Chinese Academy of Sciences, China

Title: The fate and transformation of pharmaceuticals in wetland mesocosms planted with Scirpus validusDongqing Zhang, Nanyang Technological University, Singapore

Title: Selection of phage displayed peptides for the detection of insecticide imidacloprid in soil and waterTing Xu, China Agricultural University, China


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August 18-20, 2014




Ivan Yu Sakharov, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Novel phenothiazine enhancers in chemiluminescent enzyme immunoassayIvan Yu SakharovLomonosov Moscow State University, Russia

Commonly in chemiluminescent enzyme immunoassay (CL-EIA) a light is formed upon the oxidation of luminol with hydrogen peroxide catalyzed by peroxidase used as a label of immunoreagents. Since a peroxidase is poor catalyst in

this reaction, enhancers are added to increase CL intensity. For long time the most popular enhancer was 4-iodophenol. In this work we analyzed some phenothiazine derivatives and showed that phenothiazines carrying groups with negative charge are potent primary enhancers in peroxidase-catalyzed CL, whereas phenothiazines with positive charge have no enhancing ability. The most efficient primary enhancers, whose the enhancing activity is higher many times than that of p-iodophenol, are 3-(10’-phenothiazinyl)propane-1-sulfonate (SPTZ) and 3-(10’-phenothiazinyl)propionic acid (PPA). Some pyridine derivatives are secondary enhancers increasing the enhancing ability of phenothiazines. Screening of some pyridines showed that N-morpholinopyridine (MORPH) is the most active secondary enhancer. The mechanism of its enhancing action was proposed. The conditions of the performance of the enhanced CL reaction using SPTZ or PPA in combination with MORPH as primary and secondary enhancers were optimized. The detection systems with SPTZ/PPA and MORPH were applied successfully in construction of ultrasensitive EIA kit for determination of human thyroglobulin and methylglyoxal-modified low density lipoprotein. The obtained results open good perspectives for use of ECR with phenothiazines/MORPH in the development of ultra-sensitive immunoassay kits.

BiographyIvan Yu Sakharov is a Leading Scientific Researcher at the Department of Chemistry, Lomonosov Moscow State University (Russia). He has graduated with PhD degree in Kinetics and Catalysis in 1982 and DSc degree in Biotechnology in 1992. Presently his work focuses on the development of novel sensitive chemiluminescent methods for their use in analytical practice. He has published more than 147 papers in peer-reviewed journals.

[email protected]

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August 18-20, 2014




Ming Li, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Automated small molecule bioanalytical sample preparation method developmentMing LiBiogen Idec, USA

Bioanalytical sample preparation automation has been gaining momentum for many years now, with reliable and robust automated assays being developed for most types of typical extraction procedures. With routine sample preparations

being automated, the next bottleneck in the bioanalytical workflow is sample preparation method development, which is still largely done manually as of now. On the horizon is the sample preparation method development automation, which promises to further streamline workflows and further reduce manual routine bioanalytical bench work. This trend is still in its infancy, and dedicated development staff is recommended to nurture successful application. Herein we present our recent work in the automation of the small molecule bioanalytical sample preparation method development.

BiographyMing Li is a Principal Scientist in the Translational Sciences Department at Biogen Idec, Inc., in Cambridge, MA. Formerly, he was Principal Scientist, Technology Development and Implementation at Pharmacokinetics Dynamics and Metabolism, Pfizer Global Research and Development in Groton, CT, and before that Research Scientist II at DMPK, Roche Palo Alto in Palo Alto, CA. He received his BS in Polymer Science from Fudan University in Shanghai, China and PhD in Analytical Chemistry from State University of New York at Buffalo (UB). His recent research interest and publications are in the area of bioanalytical laboratory automation.

[email protected]

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August 18-20, 2014




Ani Mulyasuryani, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Biosensor for detection of organophosphate pesticide residues by screen printed carbon electrode (SPCE)-chitosan baseAni MulyasuryaniUniversity of Brawijaya, Indonesia

Organophosphate pesticides are recommended pesticides by the Department of Agriculture, Indonesian government. Research has developed enzyme biosensor for detection organophosphate pesticide residues in agriculture products.

The biosensor based on conductance measurement of organophosphate hydrolyzed by organophosphate hydrolase (OPH). The conductance cell consisted of a pair of Screen Printed Carbon Electrode (SPCE), which is as a working and reference electrode. Working electrode is SPCE which contain immobilized OPH on chitosan membrane by cross linked method with glutaraldehyde. The area of electrodes was optimized to 3 mm2, 5 mm2 and 7 mm2. The OPH was isolated from Pseudomonas putida, that were purified by ammonium sulfate precipitation method, 6444 ppm (A) and 7865 ppm (B). The organophosphate pesticide samples were 0 to 0.1 ppm in tris-acetate buffer 0.05 M pH 8.5. The results showed that the performance of biosensor made by A enzyme is better than biosensor by B. The highest sensitivity of biosensor was resulted by electrodes of area 5 mm2. The sensitivity of biosensor in between 109-242 µS/ppm with a detection limit for each of organophosphates is 0.03 ppm (diazinon); 0.03 ppm (malathion); 0.04 ppm (chlorpyrifos), and 0.03 ppm (profenofos). The detection limit of the biosensor is lower than maximum threshold of organophosphate pesticide residues in agriculture products.

BiographyAni Mulyasuryani has completed her PhD in 2002 from Chemistry Department, Institute of Technology Bandung, Indonesia. She is the lecturer and researcher at Chemistry Department, faculty of Mathematics and Natural Sciences, University of Brawijaya, Malang, Indonesia. Now she serves as head of the chemistry master program. She developed enzyme biosensors since 2007.

[email protected]

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August 18-20, 2014




Yalavarthy Prameela Devi, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Development of bioanalytical methods for environmental monitoring of toxic chemicalsYalavarthy Prameela DeviKakatiya University, India

The talk is on biotechnological approaches for the development of low cost field kits for detection, separation, identification and quantification of toxic chemicals. Toxic chemicals can be detected, identified and quantified by various chemical

and instrumental methods, but monitoring from the environment is cumbersome, time consuming and costly. Alternatively enzymatic methods could be used in the field for monitoring of these toxic chemicals because of their exceptional performance capabilities, which include high specificity and sensitivity, rapid response, low cost and user-friendly operation. The analysis can be done in the field in an hour time. The operation cost and time is relatively less as compared with the other instrumental methods. The principle of operation also is so simple that parascientific personnel can also operate. The main principle involved in this method is the biochemical reaction between the toxic chemicals and its inhibition caused on the specific enzyme. The methodology involved was selection, extraction and standardization of enzyme activity from different sources and their inhibitory studies in the presence of different toxic chemicals for detection, identification and quantification. Paper and thin layer chromatographic methods, using enzymes and chromogenic reagents were standardized for detection and separation of various toxic chemicals. Tablet method was developed for quantification of various toxic chemicals using the same principle. Standard graphs were prepared for quantification. Based on these methods, field kits were developed for detection, separation, identification and quantification of various toxic chemicals. These developed biotechnological methods were successfully used for monitoring of toxic chemicals.

[email protected]

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August 18-20, 2014




Bonnie Yen Ping Tay, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Simple and fast analyses using GC-FID for detection of a potential genotoxins (isopropyl para-toluenesulfonate) in palm oil based esters a common ingredient used in cosmetic and personal care productsBonnie Yen Ping TayMalaysian Palm Oil Board, Malaysia

This presentation will describe a new method using GC-FID to detect isopropyl p-toluenesulfonte (IPTS) in isopropyl palmitate (IPP) and isopropyl myristate (IPM). These esters are commonly used in cosmetic and personal care (CPC)

formulation when good absorption through skin is needed. p-Toluenesulfonic acid (PTSA) is a catalyst commonly used by IPP/IPM producers during esterification of palm oil-based palmitic and palm kernel oil-based myristic acids through reaction with isopropanol (IPA). Under certain conditions, IPTS may be formed due to the tosylation reaction between IPA and PTSA. In this method, spiked IPP/IPM was directly analysed by GC without undergoing any clean up step. Calibration curves showed good linearity (0.5-50 μg mL-1, correlation coefficient: 0.9999). Recovery test revealed that the method showed excellent accuracy (IPP: 98.6%-103.5%, RSD: 0.40-2.80 % and IPM: 97.0-107.2%, RSD of 0.42-4.21%). The LOD and LOQ for spiked IPP/IPM were 12.5 μg g-1 and 25 μg g-1 respectively. The identity of IPTS in the spiked isopropyl esters was confirmed by a GC-MSD. Analyses of IPTS in commercial palm-based ester samples showed good repeatability (RSD <5%) with concentrations ranging from 34.8-1303.0 μg g-1. This is the first finding on detection of IPTS in palm-based esters, and the method can be useful as a quality check for IPP/IPM esters producers and CPC manufacturers.

BiographyBonnie Yen Ping Tay received her Bachelor of Science (pure chemistry) and Master of Science degree (organic chemistry) from University Putra Malaysia. She joined Malaysian Palm Oil Board as a research officer (RO) in 1996, majoring in analyses of palm oil phytonutrients at the Engineering and Processing Division of MPOB. In 2005, she joined another division in MPOB, Advanced Oleochemical Technology division under the Quality and Environment Assessment Unit (QEA). From 2011 till now, she holds the positions of principal research scientist. She is also the Quality Manager (MS/IEC 17025 accreditation) for QEA unit Analytical Laboratory which provides analytical services to the Malaysian palm oil industry. She is also doing research work in the field of oleochemicals method development which includes gel permeation chromatography analyses for palm-based polyols, chemometrics, e.g., measurement of hydroxyl value for palm-based polyols by NIR/FTIR spectroscopy, detection of palm oil residue in palm kernel and palm oil methyl esters by NIR and FTIR; by-products detection in oleochemicals e.g., methyl ester sulfonate and fatty alcohol ethoxylates. She was the main author of several publications in MPOB produced refereed journal, Journal of Oil Palm Research, International Journal of cosmetic science and a patent on extraction of palm carotenoids from crude palm oil.

[email protected]

Page 88



August 18-20, 2014




Shadung Moja et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Air quality challenges in Wesselton township, South AfricaShadung Moja and Zodwa NdlovuUniversity of South Africa, SouthAfrica

Wesselton Township is located within the Highveld air quality priority area in Mpumalanga Province of South Africa. The government has identified this region together with others as priority areas due to poor air quality experienced. In

this study, inhalable particulate matter of aerodynamic sizes 5 and 10 μm (PM 2.5 and PM 10), sulphur dioxide (SO2), nitogen oxides (NOx) and some volatile organic compounds (VOC’s) were analysed after measurement from an ambient air quality monitoring station. The samples were measured continuously during the dry and wet seasons in 2011, 2012 and 2013. Ambient particulate matter and the pollutant gases were measured by a light scatter method and specific gas monitors respectively.

The results were compared with both national and international standards to evaluate their levels and to monitor if the mitigating strategies adopted have any impact on the air quality. Generally, daily particulate matter standards were exceeded in some days, particularly during the dry seasons. Hourly distribution of most pollutants showed two distinct concentration patterns, large concentration peaks between 05:00 and 11:00 and relatively smaller concentration peaks between 16:00 to 22:00.

This paper will discuss some of the key results and their implication to air quality management in South Africa.

BiographyShadung Moja is currently teaching and supervising M&D research projects within the Department of Environmental Sciences (DES) at the University of South Africa (UNISA). His master’s and doctoral degrees are in Analytical Chemistry and Environmental Management respectively. He has served the academia for more than 16 years at 6 different universities. He also has 4 years of industrial chemistry experience and more than 3 years of consulting in air quality and waste management. He has published more than 15 peer reviewed articles.

[email protected]

Page 89



August 18-20, 2014




Wenxin (Shirley) Xu et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Food analysis to check quality, safety and authenticity by full-automated 1H-NMRWenxin (Shirley) Xu, Markus Link, Manfred Spraul, Hartmut Schaefer, Fang Fang and Birk SchuetzBruker BioSpin GmbH, China

Full-automated high resolution 1H-NMR spectroscopy offers unique screening capabilities for food quality and safety by combining non-targeted and targeted screening in one analysis (15 - 20 minutes from acquisition to report). Full-

automated high resolution 1H-NMR (400 MHz) has found its way into the quality control of food and beverages over the last years. The advantage of full-automated high resolution 1H-NMR is its absolute reproducibility and transferability for laboratory to laboratory, which is not equaled by other methods currently used in food analysis. NMR reproducibility allows statistical investigations e.g. for detection of variety, mixing of varieties, geographical origin and adulterations, where smallest changes of many ingredients at the same time must be recorded. Reproducibility and transferability of the solutions shown are user-, instrument- and laboratory-independent. Sample preparation, measurement and processing are based on strict standard operation procedures which are substantial for this fully automated solution. The non-targeted approach to the data allows detecting even unknown deviations, if they are visible in the 1H-NMR spectra of e.g. fruit juice, wine, edible oils or honey. The same data acquired in high throughput mode are also subjected to quantification of multiple compounds. The fully automated 1H-NMR methodology will shortly be introduced and then results on fruit juices, wine and edible oils will be presented and the advantages of the fully automated 1H-NMR solutions shown. The method has been proven on fruit juices and wine, where so far unknown frauds could be detected. In addition, conventional targeted parameters are obtained in the same analysis. This technology has additionally the advantage that NMR is completely quantitative and concentration calibration only has to be done once for all compounds.

BiographyWenxin (Shirley) Xu joined Bruker Biospin in Shanghai, China in March 2011 as NMR application scientist. She is responsible for the pre- and post-sale technology support for Bruker NMR instrument. From 2000 to 2005, she did her Bachelor’s degree in clinical medicine at Wuhan University. From 2005 to 2010 she did her Doctor’s degree in analytical chemistry in Wuhan institute of physics and mathematics, Chinese academy of science.

[email protected]

Page 90



August 18-20, 2014




Zhenxin Wang, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Bioanalytical applications of gold nanoparticle probesZhenxin WangChinese Academy of Sciences, China

In the past two decades, gold nanoparticles (GNPs) have been extensively studied and widely employed as probes for sensing/imaging wide ranges of analytes/targets, and building blocks for fabricating nanostructures and/or nanodevices. For instance,

massive GNP-based colorimetric assays have been developed for detecting different targets including metallic cations, small molecules, nucleic acids, proteins and cells, because of their unique optical properties (known as “surface plasmon resonance” (SPR) or “localized surface plasmon resonance” (LSPR)). Here, a series of functionalized GNPs have been prepared, which can be employed as probes/labels for developing microarray-based assays and colorimetric assays. We have demonstrated that these assays can be used to (i) detect metal ions (e.g., Al3+ and Pb2+) and biomolecules (e.g., Aβ1-42) both in aqueous solution and living cells, (ii) identify substrates and inhibitors of kinases, (iii) determinate binding affinity of lectin with glycans and living bacteria/cells, and (iv) study the interactions of antibiotics with bacteria/cells.

BiographyZhenxin Wang has been a Professor at Changchun Institute of Applied Chemistry (CIAC), Chinese Academy of Sciences since 2006. He graduated from Jilin University (China) in 1994 and obtained his PhD degree from CIAC at the end of 2000. As postdoctoral research fellow, he spent more than 5 years in UK. Now at CIAC, his research focuses on development of microarray-based assays and nanoparticle-based colorimetric/imaging methods for detecting biological specific recognition events. He has published more than 60 research articles in international journals including Journal of the American Chemical Society, Chemical Communications, Analytical Chemistry, Langmuir, and Biomaterials etc.

[email protected]

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August 18-20, 2014




Tie Wang, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

A crystalline self-assembly from nanords: SuperparticlesTie WangChinese Academy of Sciences, China

Self-assembly, driven by non-covalent interactions, is the fundamental mechanism behind the formation of cellular machineries that perform essential functions of life. To date, anisotropic nanoparticles have been used in the design of

directional bonding interactions on the nanometer scale through crystal-face-specific functionalization of these particles with recognition groups and/or through shape-induced anisotropic interactions. Recently, we have reported that anisotropy-driven self-assembly of CdSe/CdS semiconductor core/shell nanorods can yield needle-like superparticles with a single supercrystalline domain through a kinetic process. However, in this process, the formation of needle-like superparticles is sensitively dependent on the level of octylamine ligands on the surface of CdSe/CdS nanorods, which requires a surface treatment for incubating CdSe/CdS nanorods in a very dilute octylamine chloroform (0.1%, v/v) solution for 6 to 7 days. This requirement makes it very inconvenient to use this kinetic approach for making needle-like superparticles.

To overcome this difficulty, here we report a new synthesis for making needle-like CdSe/CdS supercrystals, which is based on the preparation of CdSe/CdS nanorods exhibiting a static structure with hydrophobic anisotropy through surface functionalization with 1,12 dodecanediamine. Because 1,12-dodecanediamine ligands are primarily functionalized onto the side faces of CdSe/CdS nanorods, their bottom and top faces exhibit more hydrophobicity than their side faces due to the hydrophilicity of amine groups. Therefore, the hydrophobic anisotropy of the resultant nanorods leads to the formation of needle-like superparticles through a process of self-assembly of these nanorods. Significantly, the surface treatment of 1,12-dodecanediamine requires only 10 min, and the quality of the resulting needle-like superparticles is comparable to that of those made using octylamine treatment for 6 to 7 days. These superparticles are important in their applications as energy-down conversion LEDs, which is important for their use as energy down-conversion phosphors in manufacturing polarized light-emitting diodes.

BiographyTie Wang has completed his PhD at the age of 28 years from Changchun Institute of Applied Chemistry Chinese Academy of Sciences and postdoctoral studies from Florida University. He is a professor of Institute of Chemistry Chinese Academy of Sciences. He has published more than 33 papers in reputed journals including Scinence, JACS, Angew Chem, PANS et al, and has been serving as an editorial board member of Journal of Analytical & Molecular Techniques.

[email protected]

Page 92



August 18-20, 2014




Dongqing Zhang, J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

The fate and transformation of pharmaceuticals in wetland mesocosms planted with Scirpus validusDongqing ZhangNanyang Technological University, Singapore

A systematic approach to assess the fate of selected pharmaceuticals (carbamazepine, naproxen, diclofenac, clofibric acid and caffeine) in wetland mesocosms was described. The overall objective of this study was to determine the kinetics of

removal and depletion of selected pharmaceuticals in mesocosms planted with S. validus growing hydroponically. The five pharmaceuticals tested including carbamazepine, naproxen, diclofenac, clofibric acid and caffeine were selected on the basis of their high occurrence in surface waters and their wide range of physicochemical properties (e.g., log Kow). The fate, removal mechanisms (i.e., photodegradation, biodegradation and plant uptake) and potential for translocation of these pharmaceuticals from the roots to the shoots was assessed using high performance liquid chromatography (HPLC). Additionally, suitable dark controls were analyzed to determine the quantitative role that photodegradation plays on pharmaceutical elimination. After 21 days of incubation, nearly all of the caffeine, naproxen and diclofenac were eliminated from solution, whereas carbamazepine and clofibric acid were recalcitrant to both photodegradation and biodegradation. Naproxen was sensitive to both photodegradation (30-42%) and biodegradation (>50%), while diclofenac was particularly sensitive (>70%) to photodegradation alone.

BiographyDongqing Zhang obtained her Master Degree at Magdeburg University (Germany) in 2005. Thereafter she completed PhD at the Dortmund University of Technology, Germany. She joined Nanyang Technological University in 2008 and is currently serving as Senior Research Fellow at Nanyang Environment and Water Research Institute, School of Civil Environmental Engineering. She has published more than 20 papers in reputed peer-reviewed journals, and Project Manager in numerous projects collaborated with local environmental agencies (Public Utility Board and National Park, Singapore).

[email protected]

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August 18-20, 2014




Ting Xu et al., J Anal Bioanal Tech 2014, 5:4http://dx.doi.org/10.4172/2155-9872.S1.017

Selection of phage displayed peptides for the detection of insecticide imidacloprid in soil and waterTing Xu1, Jianfeng Liu1, Zhiping Liu1, Kai Wang1 and Qing X Li21China Agricultural University, China2University of Hawaii at Manoa, USA

Five phage clones that selectively bind to a neonicotinoid insecticide imidacloprid have been selected from a commercialized phage display library containing linear 7-mer randomized amino acid residues. A competitive enzyme-

linked immunosorbent assay (ELISA) for imidacloprid was developed by using a clone L7-1 displaying peptides specific to the analyte. The half-maximum signal inhibition concentration (IC50) and the limit of detection (LOD) of the phage ELISA for imidacloprid were 82 ng mL-1 and 5.6 ng mL-1, respectively. This phage ELISA showed little cross-reactivities with the compounds structurally related to imidacloprid (<1%). The average recoveries of the phage ELISA for imidacloprid in soil and water samples were in a range of 74-96% and 82-91%, respectively. The simple phage-displayed peptide technology described here has been proven to be a convenient and efficient method for the development of an alternative format of ELISA for small molecules.

BiographyTing Xu got his PhD in ecology from the China Agricultural University (CAU) in 2003 and since then he has been working at CAU as a faculty member. Now he is a full professor and he focuses his research interests on the development of immunochemistry technologies for food safety, environmental pollution and ecological toxicology. He has co-authored 4 chapters of books and more than 50 papers published in international journals. He holds 5 Chinese patents.

[email protected]

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