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Oral Presentation Monday, 19th August 2019
OP-002 Studi Cemaran Lingkungan di Industri Elektronik dan Pom Bensin Berdasarkan Analisis Timbal dalam Rambut Manusia Novi Yantih ..................................................................................................................... 1
OP-004 Formulation and Characterization of α-Mangostin Polymeric Nanoparticles Based Chitosan Coated by Sodium Alginate, Sodium silicate, and Poly (Ethylene Glycol) Agus Rusdin ................................................................................................................... 2
OP-005
Evaluation of Pharmaceutical Services Support in Healthy Indonesia Framework Program with Family Approachs in West Java Zaenal Komar ................................................................................................................ 3
OP-006
Anticalculi Activity of Apigenin and Celery (Apium graveolens L.) Extract in Ethylene Glycol-Ammonium Chloride Induced Rats Sofi Nurmay Stiani ....................................................................................................... 4
OP-007
The Effect of Training About Anti-Hypertension Toward" "The Increasing Knowledge of Pharmaceutical Services by Public Health Center Pharmacist in Pandeglang Regency Yusransyah .................................................................................................................... 5
OP-008
Knowledge, Attitudes and Practices of Lung Cancer Risk Factors in West Bandung Society Mally Ghinan Sholih ..................................................................................................... 6
OP-009
Assessment of Health-Related Quality of Life Among Patients Management Program of Chronic Diseases Type 2 Diabetes Mellitus Fajriansyah .................................................................................................................... 7
OP-010
Formulasi Masker Gel Peel Off Ekstrak Kulit Pisang Ambon Lumut sebagai Antioksidan Framesti Frisma Sriarumtias ........................................................................................ 8
OP-011
Efek Brief Counseling Farmasis dan Pesan Pengingat-Motivasi Religiusitas terhadap Kualitas Hidup dan Hasil Terapi pada Pasien Diabetes Mellitus Okta Muthia Sari ............................................................................................................ 9
OP-012
The Ethanol Extract and Various Fractions of Malay Apple (Syzygium malaccense L. Merr & Perry.) Leaves as Antiobesity and Their in vitro Inhibitory Activity on Pancreatic Lipase Enzyme Atun Qowiyyah .............................................................................................................. 10
Code Title Page
iii
OP-013
Prediksi Solubilitas Kompleks Inklusi β-Siklodekstrin dengan α-Mangostin dan Turunannya melalui Simulasi Dinamika Molekul Dwina Ramadhani Pomalingo ...................................................................................... 11
OP-014
The Potential of Antihyperlipidemic Dietary Fiber in Celery (Apium graveolens L.) Aqueous Extract Residu by using in Vivo Method on Lipid Profile Parameters Nopi Rantika .................................................................................................................. 12
OP-015
Tinjauan Sistematik : Evaluasi Ekonomi Vaksinasi Hpv di Daerah yang Ditargetkan Dedi Frianto ................................................................................................................... 13
OP-016
The Effectivity of Ethanol Extract of Cortex Fructus Duku (Lansium domesticum Corr.) as a Skin Lightening Cream Lusi Nurdianti .................................................................................................................. 14
OP-017
Common Sense Self Regulatory model (CSSRM) in Diabetic Patients Using Antihypertension and/or Antihyperlipidemia in Panembahan Senopati General Hospital Bantul Yogyakarta Muthia Dewi Marthilia Alim ............................................................................................. 15
OP-018
Penetapan Kadar Aspartam dan Sakarin pada Jamu Instan untuk Anak dengan Metode KCKT Winasih Rachmawati ...................................................................................................... 16
OP-019
Dosage and Duration of Methylprednisolone Therapy Affect the Occurence of Cushing Habitus in Systemic Lupus Erythematosus Patients Didi Permana ................................................................................................................... 17
OP-020
Molekular Docking Senyawa Chalcone dan Turunannya terhadap Sel Kanker Payudara (T47D) Richa Mardianingrum ..................................................................................................... 18
OP-021
Overview of Current and Future Targets of Breast Cancer Medicines Eri Amalia ........................................................................................................................ 19
OP-022
Antioxidant Activity of Crude Bromelain of Pineapple (Ananas comosus (L.) Merr) Crown Nyi Mekar Saptarini ......................................................................................................... 20
OP-023
Lard Analysis in Sausage using FTIR Spectrophotometer Combined with Chemometrics Any Guntarti .................................................................................................................... 21
iv
OP-024
Radio-HPLC Impurities of 131I-Alpha Mangostin and 99mTC-Alpha Mangostin as Potential Breast Cancer Radiotracer Luthfi Utami Setyawati .................................................................................................... 22
OP-025
Radiolabelling of Alpha Mangostin using Iodine -131 as New Breast Cancer Diagnostic Agent Wiwit Nurhidayah ............................................................................................................ 23
OP-026
Optimization Condition for Radiolabeling Alpha Mangostin with Technetium-99m for Breast Cancer Radiodiagnostic Risda Rahmi Islamiaty .................................................................................................... 24
OP-027
Structural Desain-Based in Silico Study Of Α-Mangostin as Active Ligand Α-Estrogen Receptor Muhammad Fauzi ............................................................................................................ 25
OP-028
Improvement Dissolution Rate and Bioavailability Simvastatin Tablet Dolih Gozali ..................................................................................................................... 26
OP-029
The Effect of Bengle (Zingiber cassumunar Roxb.) Rhizome Ethanol Extract on Superosida Dismutase (Sod) Enzym Activity in Hyperlipidemic Rats Novita Sari ....................................................................................................................... 27
OP-030
Common Sense-Self Regulatory Model pada Pasien Diabetes Mellitus Tipe 2 di RSUD Abdul Azis Singkawang, RS Dok II Jayapura dan Rsud Kab. Kep. Meranti Tahun 2018 Bambang Purwoko ......................................................................................................... 28
OP-031
Atypical Antipsychotic Induced Weight Gain in Schizophrenic Patients Tetie Herlina .................................................................................................................... 29
OP-032
Pola Peresepan Obat Gastritis pada Pengobatan Mialgia di salah satu Puskesmas Kota Bandung Sri Setiatjahjati ................................................................................................................ 30
OP-033
The Use of Anti Epileptic Drugs in Children Patients in PKU Muhammadiyah Bantul General Hospital Nur Indriyastuti ............................................................................................................... 31
OP-035
Antitumor Activity of N-Hexane Fraction of Cempaka Kuning Bark (Michelia champaca Linn.) on Breast Cancer Cells Mcf7 and it’s Acute Toxicity Test Ade Zuhrotun .................................................................................................................. 32
v
OP-036
Common Sense Self Regulatory Model (CSSRM) and Medication Beliefs in Diabetics Patient using Antihypertension Medication and/or Antihyperlipidemia in Yogyakarta Regional General Hospital Andrey Wahyudi .............................................................................................................. 33
OP-038
Common Sense-Self Regulation Model pada Pasien Diabetes Mellitus yang Menggunakan Obat Antihipertensi dan/atau Antihiperlipidemia di Rumah Sakit PKU Muhammadiyah Gamping Yogyakarta Nanny Iriany Marsidin ..................................................................................................... 34
OP-039
Pengaruh Brief Counseling 5A Apoteker terhadap Pengetahuan, Persepsi, dan Kepatuhan Ibu Hamil yang Mengkonsumsi Ferrous Fumarate di Puskesmas Banguntapan III Bantul Sri Suprapti ..................................................................................................................... 35
OP-040
Anemia Prevalence Among Women of Reproductive Age in Teluk Bintuni Regency, West Papua, Indonesia Yasinta Rakanita ............................................................................................................. 36
OP-041
Anti Hair Lice Shampoo of Seed and Leaf Infusa from Pucung (Pangium edule Reinw) Ine Suharyani .................................................................................................................. 37
OP-042
Management of Look Alike and Sound Alike (Lasa) Drugs as High Alert Medicine to Reduce Dispensing Error Nur Miftahurrohmah ....................................................................................................... 38
OP-044
Out-of-Pocket Cost Structure and Determinant Factors of Economic Difficulties in Chronic Kidney Disease Patients Treated with Hemodialysis Therapy at Sentra Medika Hospital, Cibinong Prih Sarnianto ................................................................................................................. 39
OP-045
Compliance of Drug use in-Patient Hypertension Patients, One of the Rsud in Yogyakarta Private Regional Region Muhammad Muhlis .......................................................................................................... 40
OP-046
Adverse Drug Event (ADE) Kombinasi Terapi Risperidon dan Klozapin pada Pasien Skizofrenia di Rumah Sakit Jiwa Magelang Gilang Rizki Al Farizi ...................................................................................................... 41
OP-047
Validation of Porcine Gelatin using Specific Targeting on D-Loop by Real-Time Polymerase Chain Reaction Nina Salamah .................................................................................................................. 42
vi
OP-048
Selectivity Analysis of Apigenin Derivative Compounds Acting as Antioxidant using Molecular Docking Method Esti Mulatsari .................................................................................................................... 43
OP-049
The Availability of Anti-Hypertension Drug in Era of JKN: A Study in The Primary Health Care Centre Jakarta-Indonesia Mita Restinia ..................................................................................................................... 44
OP-050 Determination of Major Component of Ethyl Acetate Fraction of Katuk Leaves (Sauropus andogynus L.) Resmi Musarichie………………………………………………………………………………… 45
Poster Presentation
Code Title Page PP-001 Comparison of Hypoglicemic Effect of Extract Rinds, Fleshs, Seeds and Flowers Musa
Balbisiana Colla in a Mice
Tita Nofianti……………………………………………………………………………………...
46
PP-002 Formulation and Physical Evaluation of Chloramphenicol in Situ Gel with Base Poloxamer 188 and HPMC Insan Sunan K…………………………………………………………………………………...
47
PP-003 Quercetin of Etlingera Elatior Leaves as Cytotoxic Agent Against Hela Cell Lines
R. Herni Kusriani………………………………………………………………………………..
48
PP-004 The Effectivness of Neem Oil (Azadirachta indica A. Juss) Shampoo as Head Lice (Pediculus Humanus Capitis) Treatment
Norisca Aliza Putriana…………………………………………………………………………
49
PP-005 Analgesic and Antiinflammatory Activity of Methanol Extract and its Fractions from the Root of Polypodium Feei Mett
Deden Winda Suwandi………………………………………………………………………...
50
vii
PP-006 Processing of Catfish (Clarias garepinus) Bone Waste as a Potential Source of Gelatin for the Manufacture of Hard Capsule Shell
Devi Ratnasari…………………………………………………………………………………..
51
PP-007 Cost of Illness Study of Dengue Haemorrhagic Fever Patients One of Private Hospitals in Yogyakarta
Woro Supadmi…………………………………………………………………………………..
52
PP-008 Ethnobotanical Survey of Medicinal Plants Used in Malaria Management in Sukabumi Regency, West Jawa Province, Indonesia
Ami Tjitraresmi………………………………………………………………………………….
53
PP-009 Effect of Antipsychotic Atypical to Blood Pressure in Schizophrenia Patients Who Inpatient in Rumah Sakit Jiwa Prof. Dr. Soerojo Magelang
Haafizah Dania…………………………………………………………………………………..
54
PP-010 Efek Gastroprotektif Ekstrak Etanol Ganggang Hijau (Ulva lactuca L) Pada Lambung Tikus yang Diinduksi Etanol
Wahyu Widyaningsih…………………………………………………………………………..
55
PP-011 Moleculare Docking Studies of Coumarin Derivatives Against Ace Inhibitors as
Antihypersensitive
Riska Prasetiawati……………………………………………………………………………... 56
PP-012 Formulation of Liquid Facial Soap of Ethanol Extract of Bay Leaf’s (Syzygium polyantum W.) as Antiacne Aji Najihudin……………………………………………………………………………………..
57
PP-013 Superoxide Dismutase Activity and Histology Analysis of Male Rats After Administration of Nanoparticel of Ginger torch Flower
Ade Yeni Aprillia………………………………………………………………………………...
58
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PP-014 Aktivitas Penghambatan Enzim Elastase Ekstrak Daun Kelor (Moringa oleifera) Dan Buah Pepaya (Carica papaya L.) Pada Berbagai Variasi Konsentrasi Aseton
Dewi Ramonah…………………………………………………………………………………..
59
PP-015 Activity of Elastase Enzyme Inhibitor of Kelor Leaf Extract (Moringa oleifera) and Pepaya Fruit Extract (Carica papaya L.) in Variations of Methanol Concentration
Mohamad Usman Nur………………………………………………………………………….
60
PP-016 Preparation and Characterization of Calcium Carbonate from Eggshell (Gallus gallus Domesticus) Dadih Supriadi…………………………………………………………………………………..
61
PP-017 Daya Hambat Enzim Elastase Ekstrak Etanol Daun Kelor (Moringa oleifera L.) Dan Buah Pepaya (Carica papaya L.) dengan Variasi Konsentrasi Ristia Aprisida Rahmawati……………………………………………………………………
62
PP-018 Mixture Optimization of Papaya Fruit Extracts (Carica papaya L. Extract) and Moringga Leave (Moringa oleifera Extracts) as Sunscreen
Fathul Alimsyah………………………………………………………………………………...
63
PP-019 Antioxidant and Antiaging Activity of Pepaya Fruit Ethanol Extracts (Carica papaya L.) Aditya Noviadi Rakhmatullah………………………………………………………………...
64
PP-020 Effect of Brief Counseling-5a Modification and SMS Motivation of Religiusity on Increasing Treatment Behavior and Compliance of Patients Diabetes Mellitus
Ginanjar Zukhruf Saputri……………………………………………………………………...
65
PP-021 Kahar Method: A Novel Calculation Method of Tonicity Adjustment Abd. Kakhar Umar………………………………………………………………………………. 66
ix
PP-022 Antimicrobial Activity of Extract and Fractions of Red Algae (Gracilaria Sp.)
Rr. Sulistiyaningsih…………………………………………………………………………….
67
PP-023 Perception About Traditional Herb for Hypertension Patients in Banyumas
Aulia Rahman……………………………………………………………………………………
68
PP-024 Assesment of the Extraction Method on Phenolic Compound, Flavonoid Compound and Antioxidant Activity in Rubus fraxinifolius And R. rosifolius
Yesi Desmiaty…………………………………………………………………………………...
69
PP-025 Analisis Kadar Fenolik Total Dan Flavonoid Total Ekstrak Daun Sirih Merah (Piper crocatum Ruiz & Pav.) dengan Metode Spektrofotometer
Irma Erika Herawati…………………………………………………………………………….
70
PP-026 Comparison Nephroprotection Effect of Curcumin and Curcumin Nanoparticles on Cisplatin Induced Rats Ni Made Dwi Sandhiutami…………………………………………………………………….
71
PP-027 Nanopartikel Ekstrak Kulit Batang Kayu Manis sebagai Terapi Alternatif Antidiabetes
Mitri Asyari………………………………………………………………………………………
72
PP-028 Herbal Cair Campuran Ekstrak Daun Torbangun dan Daging Buah Asam Jawa
Sebagai Antidiabetes
Utari……………………………………………………………………………………………….
73
PP-029 Characteristics of Yogurt from Cow Milk, Sweet-Corn Milk, and Mixture Of 50% Cow Milk and 50% Sweet-Corn Milik
Erlindha Gangga………………………………………………………………………………..
74
x
PP-031 Application of High Performance Liquid Chromatography in Metabolism Studi of Isoniazid by N-Acetyltransferase-2
Novi Yantih………………………………………………………………………………………
75
PP-032 Ethanol Extract of Moringa oleifera Lam. Leaf Induced Expression of MRNA Glut-4 and Glucose Uptake in 3T3-L1 Cells
Dian Ratih Laksmitawati………………………………………………………………………
76
PP-033 Anti-Inflammation and Analgesics Effect from Infusion of Itchy Leaves (Laportea decumana (Roxb.) Wedd.) Sondang Khairani………………………………………………………………………………
77
PP-034
Anti-alopecia of Waste Cacao Skin (Theobroma cacao L.) Resmi Mustarichie……………………………………………………………………………...
78
PP-035 Examination of Quality Parameters, Toxicity Test by BSLT Method, Antioxidant Activity, and Inhibition of Α-Glucosidase Enzyme from 70% Ethanol Extract Rambutan Leaves (Nephelium lappaceum L.) Greesty Finotory Swandiny…………………………………………………………………..
79
PP-036 Determination of Quality Parameters and Antioxidant Activity Using ABTS And DPPH Methods of Kelor Leaves (Moringa oleifera L.)
Ratna Djamil……………………………………………………………………………………..
80
PP-037 The Impact of Chronic Disease Management Program (Prolanis) to Improve the Clinical Outcome of Patients with Diabetes Mellitus Type 2: A Study in Primary Health Care Center in Jakarta, Indonesia Yusi Anggriani…………………………………………………………………………………..
81
PP-038 The Perception About Traditional Medicine for Diabetes by Member of Prolanis in Banyumas Ubun Fadli Serahli……………………………………………………………………………...
82
xi
PP-039 Anti-Inflammation and Analgesic Test Effect Of 70% Ethanol Extract of Cinnamon Bark (Cinnamomum Burmanni B1.) in Vivo
Rika Sari Dewi…………………………………………………………………………………..
83
PP-040 Effect of Aspartame on Lipid Profile and Blood Glucose in White Rat
Ros Sumarny…………………………………………………………………………………… 84
PP-041 Effectiveness and Irritation Test Using Combination of Javanese Turmeric Extract (Curcuma xanthorrhiza Roxb) and Soy Bean Extract (Glycine max L. Merill)
Leni Nurlinayanti………………………………………………………………………………..
85
PP-042 Formulation of Peel-Off Gel Mask from Extract of Bisbul Leaves (Diospyros blancoi. A. Dc) as Antioxidant Fahleni……………………………………………………………………………………………
86
PP-043 Management of Look Alike and Sound Alike (Lasa) Drugs as High Alert Medicine to Reduce Dispensing Error
Nur Miftahurrohmah……………………………………………………………………………
87
PP-044 Addition of Yellow Pumpkin (Cucurbita moschata Durch) Flours as Antioxidant in Analogue Rice Based Cassava (Manihot esculenta Crantz) and Corn (Zea mays L.)
Diana Serlahwaty……………………………………………………………………………….
88
PP-045 A Brief Review Formulation and Characterization Nanostructured Lipid Carrier (NLC)
Garnadi Jafar……………………………………………………………………………………
89
PP-046 The Citotoxicity of Zingiber Ottensii Rhizome on Hela Servical Cancer Cells And MCF-7 Human Breast Cancer Cells
Lia Marliani……………………………………………………………………………………… 90
xii
PP-047 Oxidative Stress and Platelet Activation in Prehypertension Subjects: A Study on F2-Isoprostane, P-Selectin, and CD40 Ligand
Yati Sumiyati…………………………………………………………………………………….
91
PP-048 Formulasi Gel NLC Ekstrak Kalus Daun Murbei (Morus alba L.) Hasil Induksi Menggunakan Zat Pengatur Tumbuh NAA dan BAP
Faizatun…………………………………………………………………………………………..
92
PP-049 Antioxidant Effect of Brown Seaweed Extract Nanoparticles (Sargassum polycysticum) on the Model Rats a High-Fat Diet
Sarah Zaidan…………………………………………………………………………………….
93
PP-050 Anti-Hyaluronidase Activity in Vitro Test of Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm Rhizome Extract and Preparation of Solid Lipid Nanoparticles
Siti Umrah Noor…………………………………………………………………………………
94
PP-051 Marketing Strategy for Food Supplement Products
Nurita Andayani…………………………………………………………………………………
95
PP-052 The Pattern of Antidiabetic Medicines Use in X Hospital: A Study of Outpatients in the Period of January-March 2018
Moordiani………………………………………………………………………………………...
96
PP-053 Analysis of Silico Potential of Chlorogenic Acid as Anti Obesity in Receptor Human Fat Mass and Obesity Associated Protein (FTO) in Complex with 5-Carboxy-8-Hydroxyquinoline Faridah……………………………………………………………………………………………
97
PP-054 The Impact of Chronic Disease Management Program (Prolanis) to Improve the Clinical Outcome of Patients with Diabetes Mellitus Type 2: A Study in Primary Health Care Center in Jakarta, Indonesia
xiii
Reise Manninda…………………………………………………………………………………
98
PP-055 Interaction Analysis of Asiatic Acid and its Derivatives to Three Isozyme Of Nitric Acid Synthase (Nos) Using Flexible Docking Ida Musfiroh……………………………………………………………………………………..
99
PP-056 Antimicrobial Activity of Extract and Fractions of Red Algae (Gracilaria Sp.) Ade Zuhrotun……………………………………………………………………………………
100
PP-057 Patern Of Antibiotics Usage in Intensive Care Unit (ICU) of Cancer Special Hospital (CSH) in Indonesia Akrom…………………………………………………………………………………………….
101
PP-058 The Effect of Increasing Solubility with Cosolven and PVP on the Preparation and Characterization of Brown Seaweed Extract (Sargassum polycystum) Nanoparticle as Antioxidants Kartiningsih……………………………………………………………………………………...
102
PP-059 Optimization of Secreted Recombinant Human Epidermal Growth Factor Production Using Pectate Lyase B from Escherichia Coli Bl21(DE3) by Central Composite Design and its Production in High Cell Density Culture
Sriwidodo………………………………………………………………………………………...
103
PP-060 Etlingera elatior Flower Oil Analysis and its Protection Level Test in Mosquito Repellent Lotion Lilis Tuslinah…………………………………………………………………………………….
104
PP-061 Single Nucleotide Polymorphism of CTLA-4 (RS5742909) in Correlation with Schizophrenia Risk Factor Riyadi……………………………………………………………………………………………..
105 PP-062 Genotype Identification and Effect of Genetic Polymorphism of TPH2 RS120074175
Gene on Risk Factors of Scizophrenia
xiv
PP-064 Quality of Life of Hypertensive Patients with Candesartan and Candesartan-Amlodipine Combination Therapy at A Governmental Hospital in Yogyakarta, Indonesia
Faridah Baroroh……………………………………………………………………………….. 107 PP-065 Penetapan Kadar Fenol Dan Antosianin Total Ekstrak Etanol 96% Beras Merah (Oryza
sativa L.) Secara Spektrofotometri Hari Susanti……………………………………………………………………………………. 108
PP-066 Penetapan Parameter Mutu dan Uji Aktivitas Antioksidan dari Ekstrak Rimpang
Lengkuas Belang (Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm) Wiwi Winarti…………………………………………………………………………………….. 109
James Prasetyo Laksono……………………………………………………………………..
106
xv
SCHEDULE OF 2nd POSTGRADUATE SEMINAR ON PHARMACEUTICAL SCIENCES 2019 (2nd
PSPS 2019)
Ballroom Savoy Homan Hotel, 19th August 2019
Time Schedule
08.00-08.30 Registration
08.30-09.00 Opening ceremony
09.00-09.30 Coffee break
09.30-10.30 Keynote Speakers : Prof. Shaharum Shamsuddin
10.00-12.00 Plenary Lecture 1 : Dr. Dra. Iis Wahyuningsih M.Si., Apt
Plenary Lecture 2 : Neily Zakiyah Ph.D., Apt
12.00-13.00 Lunch Break
13.00-15.30 Parallel Session
15.30-16.00 Closing ceremony
xvi
Oral Presentration
Room I, Monday 19th August 2019
Time Code Presenter
Name Title
13.00-13.10
OP-002
Novi Yantih Studi Cemaran Lingkungan di Industri Elektronik dan Pom Bensin Berdasarkan Analisis Timbal dalam Rambut Manusia
13.10-13.20
OP-004
Agus Rusdin Formulation and Characterization of α-Mangostin Polymeric Nanoparticles Based Chitosan Coated by Sodium Alginate, Sodium silicate, and Poly (Ethylene Glycol)
13.20-13.30
OP-005
Zaenal Komar Evaluation of Pharmaceutical Services Support in Healthy Indonesia Framework Program with Family Approachs in West Java
13.30-13.40
OP-006
Sofi Nurmay Stiani
Anticalculi Activity of Apigenin and Celery (Apium graveolens L.) Extract in Ethylene Glycol-Ammonium Chloride Induced Rats
13.40-13.50
OP-007
Yusransyah The Effect of Training About Anti-Hypertension Toward" "The Increasing Knowledge of Pharmaceutical Services by Public Health Center Pharmacist in Pandeglang Regency
13.50-14.00
OP-008
Mally Ghinan Sholih
Knowledge, Attitudes and Practices of Lung Cancer Risk Factors in West Bandung Society
14.00-14.10
OP-009
Fajriansyah Assessment of Health-Related Quality of Life Among Patients Management Program of Chronic Diseases Type 2 Diabetes Mellitus
14.10-14.20
OP-010
Framesti Frisma Sriarumtias
Formulasi Masker Gel Peel Off Ekstrak Kulit Pisang Ambon Lumut sebagai Antioksidan
14.20-14.30
OP-011
Okta Muthia Sari
Efek Brief Counseling Farmasis dan Pesan Pengingat-Motivasi Religiusitas terhadap Kualitas Hidup dan Hasil Terapi pada Pasien Diabetes Mellitus
14.30-14.40
OP-012
Atun Qowiyyah The Ethanol Extract and Various Fractions of Malay Apple (Syzygium malaccense L. Merr & Perry.) Leaves as Antiobesity and Their in vitro Inhibitory Activity on Pancreatic Lipase Enzyme
14.40-14.50
OP-013
Dwina Ramadhani Pomalingo
Prediksi Solubilitas Kompleks Inklusi β-Siklodekstrin dengan α-Mangostin dan Turunannya melalui Simulasi Dinamika Molekul
14.50-15.00
OP-014
Nopi Rantika The Potential of Antihyperlipidemic Dietary Fiber in Celery (Apium graveolens L.) Aqueous Extract Residu by using in Vivo Method on Lipid Profile Parameters
15.00-15.10
OP-015
Dedi Frianto Tinjauan Sistematik : Evaluasi Ekonomi Vaksinasi Hpv di Daerah yang Ditargetkan
15.10-15.20
OP-016
Lusi Nurdianti The Effectivity of Ethanol Extract of Cortex Fructus Duku (Lansium domesticum Corr.) as a Skin Lightening Cream
15.20-15.30
OP-017
Muthia Dewi Marthilia Alim
Common Sense Self Regulatory model (CSSRM) in Diabetic Patients Using Antihypertension and/or Antihyperlipidemia in Panembahan Senopati General Hospital Bantul Yogyakarta
xvii
Oral Presentration
Room II, Monday 19th August 2019
Time Code Presenter
Name Title
13.00-13.10
OP-049
Mita Restinia The Availability of Anti-Hypertension Drug in Era of JKN: A Study in The Primary Health Care Centre Jakarta-Indonesia
13.10-13.20
OP-019
Didi Permana Dosage and Duration of Methylprednisolone Therapy Affect the Occurence of Cushing Habitus in Systemic Lupus Erythematosus Patients
13.20-13.30
OP-020
Richa Mardianingrum
Molekular Docking Senyawa Chalcone dan Turunannya terhadap Sel Kanker Payudara (T47D)
13.30-13.40
OP-021
Eri Amalia Overview of Current and Future Targets of Breast Cancer Medicines
13.40-13.50
OP-022
Nyi Mekar Saptarini
Antioxidant Activity of Crude Bromelain of Pineapple (Ananas comosus (L.) Merr) Crown
13.50-14.00
OP-023
Any Guntarti Lard Analysis in Sausage using FTIR Spectrophotometer Combined with Chemometrics
14.00-14.10
OP-024
Luthfi Utami Setyawati .
Radio-HPLC Impurities of 131I-Alpha Mangostin and 99mTC-Alpha Mangostin as Potential Breast Cancer Radiotracer
14.10-14.20
OP-025
Wiwit Nurhidayah
Radiolabelling of Alpha Mangostin using Iodine -131 as New Breast Cancer Diagnostic Agent
14.20-14.30
OP-026
Risda Rahmi Islamiaty
Optimization Condition for Radiolabeling Alpha Mangostin with Technetium-99m for Breast Cancer Radiodiagnostic
14.30-14.40
OP-027
Muhammad Fauzi
Structural Desain-Based in Silico Study Of Α-Mangostin as Active Ligand Α-Estrogen Receptor
14.40-14.50
OP-028
Dolih Gozali Improvement Dissolution Rate and Bioavailability Simvastatin Tablet
14.50-15.00
OP-029
Novita Sari The Effect of Bengle (Zingiber cassumunar Roxb.) Rhizome Ethanol Extract on Superosida Dismutase (Sod) Enzym Activity in Hyperlipidemic Rats
15.00-15.10
OP-030
Bambang Purwoko
Common Sense-Self Regulatory Model pada Pasien Diabetes Mellitus Tipe 2 di Rsud Abdul Azis Singkawang, Rs Dok II Jayapura dan Rsud Kab. Kep. Meranti Tahun 2018
15.10-15.20
OP-031
Tetie Herlina Atypical Antipsychotic Induced Weight Gain in Schizophrenic Patients
15.20-15.30
OP-032
Sri Setiatjahjati Pola Peresepan Obat Gastritis pada Pengobatan Mialgia di salah satu Puskesmas Kota Bandung
xviii
Oral Presentration
Room III, Monday 19th August 2019
Time Code Presenter
Name Title
13.00-13.10
OP-033
Nur Indriyastuti The Use of Anti Epileptic Drugs in Children Patients in PKU Muhammadiyah Bantul General Hospital
13.10-13.20
OP-035
Ade Zuhrotun Antitumor Activity of N-Hexane Fraction of Cempaka Kuning Bark (Michelia champaca Linn.) on Breast Cancer Cells Mcf7 and it’s Acute Toxicity Test
13.20-13.30
OP-036
Andrey Wahyudi
Common Sense Self Regulatory Model (CSSRM) and Medication Beliefs in Diabetics Patient using Antihypertension Medication and/or Antihyperlipidemia in Yogyakarta Regional General Hospital
13.30-13.40
OP-038
Nanny Iriany Marsidin
Common Sense-Self Regulation Model pada Pasien Diabetes Mellitus yang Menggunakan Obat Antihipertensi dan/atau Antihiperlipidemia di Rumah Sakit PKU Muhammadiyah Gamping Yogyakarta
13.40-13.50
OP-039
Sri Suprapti Pengaruh Brief Counseling 5A Apoteker terhadap Pengetahuan, Persepsi, dan Kepatuhan Ibu Hamil yang Mengkonsumsi Ferrous Fumarate di Puskesmas Banguntapan III Bantul
13.50-14.00
OP-040
Yasinta Rakanita
Anemia Prevalence Among Women of Reproductive Age in Teluk Bintuni Regency, West Papua, Indonesia
14.00-14.10
OP-041
Ine Suharyani Anti Hair Lice Shampoo of Seed and Leaf Infusa from Pucung (Pangium edule Reinw)
14.10-14.20
OP-042
Nur Miftahurrohmah
Management of Look Alike and Sound Alike (Lasa) Drugs as High Alert Medicine to Reduce Dispensing Error
14.20-14.30
OP-044
Prih Sarnianto Out-of-Pocket Cost Structure and Determinant Factors of Economic Difficulties in Chronic Kidney Disease Patients Treated with Hemodialysis Therapy at Sentra Medika Hospital, Cibinong
14.30-14.40
OP-045
Muhammad Muhlis
Compliance of Drug use in-Patient Hypertension Patients, One of the Rsud in Yogyakarta Private Regional Region
14.40-14.50
OP-046
Gilang Rizki Al Farizi
Adverse Drug Event (ADE) Kombinasi Terapi Risperidon dan Klozapin pada Pasien Skizofrenia di Rumah Sakit Jiwa Magelang
14.50-15.00
OP-047
Nina Salamah Validation of Porcine Gelatin using Specific Targeting on D-Loop by Real-Time Polymerase Chain Reaction
15.00-15.10
OP-048
Esti Mulatsari Selectivity Analysis of Apigenin Derivative Compounds Acting as Antioxidant using Molecular Docking Method
15.10-15.20
OP-018
Winasih Rachmawati
Penetapan Kadar Aspartam dan Sakarin pada Jamu Instan untuk Anak dengan Metode KCKT
15.20-15.30
OP-050
Resmi Mustarichie
Determination of Major Component of Ethyl Acetate Fraction of Katuk Leaves (Sauropus andogynus L.)
xix
Poster Presentation
Monday, 19th August 2019
Code Presenter Name Title
PP-001 Tita Nofianti Comparison of Hypoglicemic Effect of Extract Rinds, Fleshs, Seeds and Flowers Musa Balbisiana Colla in a Mice
PP-002 Insan Sunan K Formulation and Physical Evaluation of Chloramphenicol in Situ Gel with Base Poloxamer 188 and HPMC
PP-003 R. Herni Kusriani Quercetin of Etlingera Elatior Leaves as Cytotoxic Agent Against Hela Cell Lines
PP-004 Norisca Aliza Putriana The Effectivness of Neem Oil (Azadirachta indica A. Juss) Shampoo as Head Lice (Pediculus Humanus Capitis) Treatment
PP-005 Deden Winda Suwandi Analgesic and Antiinflammatory Activity of Methanol Extract and its Fractions from the Root of Polypodium Feei Mett
PP-006 Devi Ratnasari Processing of Catfish (Clarias garepinus) Bone Waste as a Potential Source of Gelatin for the Manufacture of Hard Capsule Shell
PP-007 Woro Supadmi Cost of Illness Study of Dengue Haemorrhagic Fever Patients One of Private Hospitals in Yogyakarta
PP-008 Ami Tjitraresmi Ethnobotanical Survey of Medicinal Plants Used in Malaria Management in Sukabumi Regency, West Jawa Province, Indonesia
PP-009 Haafizah Dania Effect of Antipsychotic Atypical to Blood Pressure in Schizophrenia Patients Who Inpatient in Rumah Sakit Jiwa Prof. Dr. Soerojo Magelang
PP-010 Wahyu Widyaningsih Efek Gastroprotektif Ekstrak Etanol Ganggang Hijau (Ulva lactuca L) Pada Lambung Tikus yang Diinduksi Etanol
PP-011 Riska Prasetiawati Moleculare Docking Studies of Coumarin Derivatives Against Ace Inhibitors as Antihypersensitive
PP-012 Aji Najihudin Formulation of Liquid Facial Soap of Ethanol Extract of Bay Leaf’s (Syzygium polyantum W.) as Antiacne
PP-013 Ade Yeni Aprillia Superoxide Dismutase Activity and Histology Analysis of Male Rats After Administration of Nanoparticel of Ginger torch Flower
PP-014 Dewi Ramonah Aktivitas Penghambatan Enzim Elastase Ekstrak Daun Kelor (Moringa oleifera) Dan Buah Pepaya (Carica papaya L.) Pada Berbagai Variasi Konsentrasi Aseton
PP-015 Mohamad Usman Nur Activity of Elastase Enzyme Inhibitor of Kelor Leaf Extract (Moringa oleifera) and Pepaya Fruit Extract (Carica papaya L.) in Variations of Methanol Concentration
PP-016 Dadih Supriadi Preparation and Characterization of Calcium Carbonate from Eggshell (Gallus gallus Domesticus)
xx
PP-017 Ristia Aprisida Rahmawati Daya Hambat Enzim Elastase Ekstrak Etanol Daun Kelor (Moringa oleifera L.) Dan Buah Pepaya (Carica papaya L.) dengan Variasi Konsentrasi
PP-018 Fathul Alimsyah Mixture Optimization of Papaya Fruit Extracts (Carica papaya L. Extract) and Moringga Leave (Moringa oleifera Extracts) as Sunscreen
PP-019 Aditya Noviadi Rakhmatullah
Antioxidant and Antiaging Activity of Pepaya Fruit Ethanol Extracts (Carica papaya L.)
PP-020 Ginanjar Zukhruf Saputri Effect of Brief Counseling-5a Modification and SMS Motivation of Religiusity on Increasing Treatment Behavior and Compliance of Patients Diabetes Mellitus
PP-021 Abd. Kakhar Umar Kahar Method: A Novel Calculation Method of Tonicity Adjustment
PP-022 Rr. Sulistiyaningsih Antimicrobial Activity of Extract and Fractions of Red Algae (Gracilaria Sp.)
PP-023 Aulia Rahman Perception About Traditional Herb for Hypertension Patients in Banyumas
PP-024 Yesi Desmiaty Assesment of the Extraction Method on Phenolic Compound, Flavonoid Compound and Antioxidant Activity in Rubus fraxinifolius And R. rosifolius
PP-025 Irma Erika Herawati Analisis Kadar Fenolik Total Dan Flavonoid Total Ekstrak Daun Sirih Merah (Piper crocatum Ruiz & Pav.) dengan Metode Spektrofotometer
PP-026 Ni Made Dwi Sandhiutami Comparison Nephroprotection Effect of Curcumin and Curcumin Nanoparticles on Cisplatin Induced Rats
PP-027 Mitri Asyari Nanopartikel Ekstrak Kulit Batang Kayu Manis sebagai Terapi Alternatif Antidiabetes
PP-028 Utari Herbal Cair Campuran Ekstrak Daun Torbangun dan Daging Buah Asam Jawa Sebagai Antidiabetes
PP-029 Erlindha Gangga Characteristics of Yogurt from Cow Milk, Sweet-Corn Milk, and Mixture Of 50% Cow Milk and 50% Sweet-Corn Milik
PP-031 Novi Yantih Application of High Performance Liquid Chromatography in Metabolism Studi of Isoniazid by N-Acetyltransferase-2
PP-032 Dian Ratih Laksmitawati Ethanol Extract of Moringa oleifera Lam. Leaf Induced Expression of MRNA Glut-4 and Glucose Uptake in 3T3-L1 Cells
PP-033 Sondang Khairani Anti-Inflammation and Analgesics Effect from Infusion of Itchy Leaves (Laportea decumana (Roxb.) Wedd.)
PP-034 Resmi Mustarichie Anti-alopecia of Waste Cacao Skin (Theobroma cacao L.)
PP-035 Greesty Finotory Swandiny
Examination of Quality Parameters, Toxicity Test by BSLT Method, Antioxidant Activity, and Inhibition of Α-Glucosidase Enzyme from 70% Ethanol Extract Rambutan Leaves (Nephelium lappaceum L.)
PP-036 Ratna Djamil Determination of Quality Parameters and Antioxidant Activity Using ABTS And DPPH Methods of Kelor Leaves (Moringa oleifera L.)
xxi
PP-037 Yusi Anggriani
The Impact of Chronic Disease Management Program (Prolanis) to Improve the Clinical Outcome of Patients with Diabetes Mellitus Type 2: A Study in Primary Health Care Center in Jakarta, Indonesia
PP-038 Ubun Fadli Serahli The Perception About Traditional Medicine for Diabetes by Member of Prolanis in Banyumas
PP-039 Rika Sari Dewi Anti-Inflammation and Analgesic Test Effect Of 70% Ethanol Extract of Cinnamon Bark (Cinnamomum Burmanni B1.) in Vivo
PP-040 Ros Sumarny Effect of Aspartame on Lipid Profile and Blood Glucose in White Rat
PP-041 Leni Nurlinayanti Effectiveness and Irritation Test Using Combination of Javanese Turmeric Extract (Curcuma xanthorrhiza Roxb) and Soy Bean Extract (Glycine max L. Merill)
PP-042 Fahleni Formulation of Peel-Off Gel Mask from Extract of Bisbul Leaves (Diospyros blancoi. A. Dc) as Antioxidant
PP-043 Nur Miftahurrohmah Management of Look Alike and Sound Alike (Lasa) Drugs as High Alert Medicine to Reduce Dispensing Error
PP-044 Diana Serlahwaty Addition of Yellow Pumpkin (Cucurbita moschata Durch) Flours as Antioxidant in Analogue Rice Based Cassava (Manihot esculenta Crantz) and Corn (Zea mays L.)
PP-045 Garnadi Jafar A Brief Review Formulation and Characterization Nanostructured Lipid Carrier (NLC)
PP-046 Lia Marliani The Citotoxicity of Zingiber Ottensii Rhizome on Hela Servical Cancer Cells And MCF-7 Human Breast Cancer Cells
PP-047 Yati Sumiyati Oxidative Stress and Platelet Activation in Prehypertension Subjects: A Study on F2-Isoprostane, P-Selectin, and CD40 Ligand
PP-048 Faizatun Formulasi Gel NLC Ekstrak Kalus Daun Murbei (Morus alba L.) Hasil Induksi Menggunakan Zat Pengatur Tumbuh NAA dan BAP
PP-049 Sarah Zaidan Antioxidant Effect of Brown Seaweed Extract Nanoparticles (Sargassum polycysticum) on the Model Rats a High-Fat Diet
PP-050 Siti Umrah Noor Anti-Hyaluronidase Activity in Vitro Test of Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm Rhizome Extract and Preparation of Solid Lipid Nanoparticles
PP-051 Nurita Andayani Marketing Strategy for Food Supplement Products
PP-052 Moordiani The Pattern of Antidiabetic Medicines Use in X Hospital: A Study of Outpatients in the Period of January-March 2018
PP-053 Faridah
Analysis of Silico Potential of Chlorogenic Acid as Anti Obesity in Receptor Human Fat Mass and Obesity Associated Protein (FTO) in Complex with 5-Carboxy-8-Hydroxyquinoline
PP-054 Reise Manninda
The Impact of Chronic Disease Management Program (Prolanis) to Improve the Clinical Outcome of Patients with Diabetes Mellitus Type 2: A Study in Primary Health Care Center in Jakarta, Indonesia
xxii
PP-055 Ida Musfiroh Interaction Analysis of Asiatic Acid and its Derivatives to Three Isozyme of Nitric Acid Synthase (NOS) Using Flexible Docking
PP-056 Ade Zuhrotun Antimicrobial Activity of Extract and Fractions of Red Algae (Gracilaria Sp.)
PP-057 Akrom Patern Of Antibiotics Usage in Intensive Care Unit (ICU) of Cancer Special Hospital (CSH) in Indonesia
PP-058 Kartiningsih
The Effect of Increasing Solubility with Cosolven and PVP on the Preparation and Characterization of Brown Seaweed Extract (Sargassum polycystum) Nanoparticle as Antioxidants
PP-059 Sriwidodo
Optimization of Secreted Recombinant Human Epidermal Growth Factor Production Using Pectate Lyase B from Escherichia Coli Bl21(DE3) by Central Composite Design and its Production in High Cell Density Culture
PP-060 Lilis Tuslinah Etlingera elatior Flower Oil Analysis and its Protection Level Test in Mosquito Repellent Lotion
PP-061 Riyadi Single Nucleotide Polymorphism of CTLA-4 (RS5742909) in Correlation with Schizophrenia Risk Factor
PP-062 James Prasetyo Laksono Genotype Identification and Effect of Genetic Polymorphism of TPH2 RS120074175 Gene on Risk Factors of Scizophrenia
PP-064 Faridah Baroroh Quality of Life of Hypertensive Patients with Candesartan and Candesartan-Amlodipine Combination Therapy at A Governmental Hospital in Yogyakarta, Indonesia
PP-065 Hari Susanti Penetapan Kadar Fenol Dan Antosianin Total Ekstrak Etanol 96% Beras Merah (Oryza sativa L.) Secara Spektrofotometri
PP-066 Wiwi Winarti Penetapan Parameter Mutu dan Uji Aktivitas Antioksidan dari Ekstrak Rimpang Lengkuas Belang (Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm)
xxiii
Health Economic Evaluation of Family Planning Interventions in Low and Middle Income
Countries; Systematic Review and Decision Modelling Analysis.
Neily Zakiyah
Abstract
Background
A significant number of women in low and middle income countries (L-MICs) who need any family
planning, experience a lack in access to modern effective methods. The aim of this research is to estimate
the cost-effectiveness of scaling up family planning interventions to reduce the unmet need in L-MICs
using systematic review and decision analytic modelling, with Indonesia and Uganda as reference cases.
Methods
Systematic review: A systematic review was performed in several electronic databases, and data was
synthesized and analyzed using a narrative approach. The reporting quality of the included studies was
assessed using the Consolidated Health Economic Evaluation Reporting Standards (CHEERS)
statement.
Cost-utility analysis: A Markov decision analytic model was developed, where current situation and
several scenarios to reduce the unmet need were incorporated as the comparative strategies. Country-
specific evidences were synthesized from the Demographic and Health Survey (DHS) and published
studies. The model simulated the natural phase of reproductive health experience of women in the
reproductive age over a lifetime time horizon. Modeled outcomes included clinical events, costs, and
incremental cost effectiveness ratios expressed as cost per disability adjusted life year averted (ICERs
per DALY). Deterministic and probabilistic sensitivity analyses were conducted to assess the impact of
parameter uncertainty on modeled outcomes.
Results
Systematic review: Assembled evidence from the review suggested that improving family planning
interventions can be cost effective in a variety of L-MICs, as measured against accepted international
cost effectiveness benchmarks; however, the evidence is only supported by a very limited number of
studies. The major drivers of cost effectiveness were cost of increasing coverage, effectiveness of the
interventions and country-specific factors.
Cost-utility analysis: In the hypothetical cohort of 100,000 women, scenarios to reduce the unmet need
for family planning would result in savings within a range of US$ 230,600 – US$ 895,100 and US$564,400
– US$1,865,900 in Indonesia and Uganda, respectively. The interventions would avert an estimated 1,859
xxiv
– 3,780 and 3,705 – 12,230 DALYs in Indonesia and Uganda, respectively. The results of our analysis
indicate that scaling up family planning dominates the current situation in all scenarios in both countries,
with lower costs and fewer DALYs. These results were robust in sensitivity analyses.
Conclusion: Scaling up family planning interventions could improve women’s health outcomes
substantially and be cost-effective or even cost-saving across a range of scenarios compared to the
current situation.
xxv
Upaya Peningkatan Bioavailabilitas Obat Melalui Self Nanoemulsion Drug Delivery System
Iis Wahyuningsih, Fakultas Farmasi, Universitas Ahmad Dahlan, Yogyakarta.
Pemberian obat secara peroral adalah jalur pemberian termudah dan paling nyaman. Saat ini lebih dari
40% senyawa kimia baru menunjukkan kelarutan dalam air yang buruk, hal tersebut akan menghasilkan
bioavailabilitas yang tidak memuaskan dan selanjutnya aktivitas yang tidak optimal. Peningkatan
bioavailabilitas obat adalah salah satu tantangan besar dalam formulasi obat. Self Nanoemulsi Drug
Delivery System (SNEDDS) merupakan salah satu strategi yang menjanjikan untuk meningkatkan jumlah
dan kecepatan absorpsi secara peroral. SNEDDS adalah campuran minyak, surfaktan dan cosolven/ko-
surfaktan. Prinsip dari sistem ini adalah kemampuannya dalam membentuk emulsi minyak dalam air (o/w)
dalam ukuran nanoemulsi pada agitasi ringan oleh motilitas lambung/usus setelah bertemu dengan
medium disolusi di sistem gastointestinal. Karena ukuran dropletnya sangat kecil, akan menyebabkan
peningkatan disolusi obat, difusi dan selanjutnya bioavailabilitas. Furosemid adalah diuretik yang masuk
dalam kategori Biopharmaceutical Class System (BCS) kelas IV, sedangkan piroksikam adalah
analgetik-antiinflamasi yang masuk dalam kategori BCS kelas II. Kajian ini akan difokuskan pada tahapan
formulasi SNEDDS furosemid dan SNEDDS piroksikam, karakterisasi, peningkatan disolusi, difusi,
bioavailabilitas, aktivitas dan penurunan efek samping kedua obat tersebut dibandingkan dengan non-
SNEDDS.
Kata kunci : SNEDDS, furosemid, piroksikam
xxvi
In Vitro Evaluation of Nanocarrier For Drug Delivery Frug
Shaharum Shamsuddin School of Health Sciences
USM Health Campus, 16150 Kubang Kerian, Kelantan
There are various benefits of encapsulating drugs into nanocarriers such as nanoparticles for drug
delivery purposes. For instance, by reducing the size of the drugs into nano sized formulations, the drug
dissolution rate can be increased, thereby improving the drug absorption and bioavailability in the body.
Apart from that, drugs can be delivered at the desired site of action in the human body, thereby improving
the therapeutic index of the drugs by increasing their efficiency and reducing their toxicity. For example,
chemotherapeutic drugs administered at high dosages in the human body to destroy tumour cells can
inadvertently destroy the surrounding healthy tissues or cells as well, thereby eliciting unwanted ill effects.
Another major advantage of employing nanoparticles to deliver drugs is to provide protection for easily
degraded or short half-life drugs, such as small peptides and nucleic acids to prolong the pharmacological
effects. Nanoparticles are materials with dimensions at the nanoscale which ranges roughly from 1 to 100
nanometers (nm). Properties of nanoparticles are highly dependent on size, shape and chemistry of the
compound. At such small scale ranges, nanoparticles can take on radically different properties not seen
in their bulk counterparts. Cytotoxicity is quality of being toxic to cells. In order for nanoparticles to be
used safely in human, it is essential to test its cytotoxicity in cell lines. The cytotoxicity test of nanoparticles
was carried out on the human colon cancer cell lines (HCT 116) using a WST-1 cell proliferation reagent.
The assay is based on cleavage of the tetrazolium salt WST-1 by active mitochondria to produce a soluble
colored formazan salt. Since the conversion is operated only by viable cells, it directly correlates with the
cell number. The outcome of this test will help to balance the toxicity and the efficacy properties of the
synthesized nanoparticles on the cancer cell lines HCT 116 used. However, this test is also essential for
all types of nanoparticles to be used in biomedical applications. It is important to note that cytotoxicity test
can be carried out using different nanoparticles, cell lines and reagents.
1
Oral Presentation [OP-002]
ENVIRONMENTAL POLLUTION STUDY IN ELECTRONIC INDUSTRY AND GAS STATION BASED ANALYSIS OF LEAD IN HUMAN HAIR
Novi Yantih, Dita Apriani Pertiwi
Fakultas Farmasi, Universitas Pancasila
Korespondensi: [email protected], 08129624502
ABSTRACT
Background: Heavy metals can be bio-accumulated in human hair. This bioaccumulation can be an indicator of
heavy metal pollution in an environment. One of the heavy metals that pollute the environment is lead (Pb)
Objective: The purpose of this study was to study contamination of the environment in the electronics industry
and gas stations based on analysis of Pb levels on the hair of workers. Method: The study of heavy metal
contamination was carried out by analyzing Pb on the hair of 6 workers in each environment using atomic
absorption spectrophotometry (AAS) method. The method was validated before use. Results: The results of the
method validation show that the AAS method has LLOQ of 0.1 μg/mL with the linearity, accuracy, precision, and
selectivity that meet the requirements. The results of Pb level analysis on workers' hair in electronics industrial
and gas stations were 0.5126 and 0.4458 g/g, respectively. Statistical analysis showed no significant difference
between Pb metal contamination in workers' hair in both environments. Bioaccumulation of Pb metal contamination
in workers' hair in both environments was still eligible because the levels are below the maximum limit (8.800
g/mL) permitted by the GbW 07601 Certified Human Hair Reference Material, IAEA. Conclusion: The electronics
industrial environment showed Pb metal contamination which was not significantly different from the gas station
environment. Pb metal contamination in both environments was still below the maximum limit.
Keywords: Human Hair, Lead, Industry, Gas Station
2
Oral Presentation [OP-004]
FORMULATION AND CHARACTERIZATION OF α-MANGOSTIN POLYMERIC
NANOPARTICLES BASED CHITOSAN COATED BY SODIUM ALGINATE, SODIUM SILICATE, AND POLY(ETHYLENE GLYCOL)
Agus Rusdin1, Nasrul Wathoni1*, I Made Joni2, Ronny Lesmana3, Keiichi Motoyama4,
Muchtaridi5
1Department of Pharmaceutics, Faculty of Pharmacy, Padjadjaran University, Sumedang, Indonesia.
2Department of Physics, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Sumedang, Indonesia.
3Department of Anatomy, Physiology and Biology Cell, Faculty of Medicine, UniversitasPadjadjaran, Sumedang, Indonesia
4Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan 5Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Padjadjaran
University, Sumedang, Indonesia.
Corresponding author: email [email protected]
ABSTRACT
Background: α-mangostin, one of the xanthone derivative compounds isolated from Garcinia mangostana L. peel
extract, has an excellent anticancer efficacy. However, α-mangostin has a lack site specificity, a poor cells
selectivity, and a low aqueous solubility. Polymeric nanoparticles formulation can be used to solve these problems.
Therefore, the main objective of this study was to develop polymeric nanoparticles of α-mangostin based chitosan
(αM-Ch) coated by sodium alginate (αM-Ch/Al), sodium silicate (αM-Ch/Si), and poly(ethylene glycol) 6000 (αM-
Ch/PEG). Materials and Methods: Polymeric nanoparticles were prepared by ionic gelation method with the spray
pyrolysis technique. Characterization of polymeric nanoparticles was analyzed by scanning electron microscope
(SEM), fourier transform infrared (FTIR), differential scanning calorimetry (DSC), X-ray diffraction (XRD) and
entrapment efficiency by UV-Vis Spectrophotometry. Results: The results showed that αM-Ch/Al, αM-Ch/Si, and
αM-Ch/PEG were successfully prepared with the range of particle size about 200-400 nm. FTIR study confirmed
that α-mangostin was succesfully loaded in polymeric nanoparticles. Interestingly, the XRD patterns and DSC
thermograms of αM-Ch/Al showed an amorphous state, whereas αM-Ch/Si and αM-Ch/PEG indicated a crystaline
forms. In addition, αM-Ch/Al had the highest entrapment efficiency (98.33±0.06) compared to αM-Ch/Si
(70.46±8.93), and αM-Ch/PEG (92.24±10.98). Conclusion: These results suggest that αM-Ch/Al has a potential
to enhance the physicochemical properties of α-mangostin for anticancer therapy.
Keywords: α-Mangostin, Polymeric Nanoparticle, Anticancer, Chitosan, Alginate, Silicate, Poly(Ethylene Glycol)
3
Oral Presentation [OP-005]
EVALUATION OF PHARMACEUTICAL SERVICES SUPPORT IN HEALTHY INDONESIA FRAMEWORK PROGRAM WITH FAMILY APPROACHS IN WEST JAVA
Zaenal Komar 1,2, Keri Lestari2,4, Ali Gufron Mukti 3, Yana Irawati 2, Miftahudiniyah2
1Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran,
Jatinangor-Sumedang, West Java, Indonesia;
2 Jakarta National Health Training Centre, Jakarta Indonesia;
3 Ministry of Research, Technology and Higher Education of the Republic of Indonesia
Corresponding Author: [email protected]
ABSTRACT
Background and Objectives: National Health Insurance (JKN) has been implemented in Indonesia since January
1, 2014. Health centers are one of the pillars of the successful implementation of JKN. In addition, the Community
Health Center is an important component in the Healthy Indonesia Program with the exit approach (PIS-PK). One
important component in service at the Health Center is Pharmaceutical Services. The purpose of this study was
to determine the support of Pharmaceutical Services in Healthy Indonesia Program with a Family Approach in the
implementation National Health Insurance (JKN). Material and Method: This research method is cross sectional
with simultaneous observations. Data collected were retrospective in 2018. Research was conducted on 40
Community health center (Puskesmas) located in West Java with the Inclusive Criteria : Community health center
(PKM) was accredited, Participated in Healthy Indonesia Program (PK PIS) Training and Actively conducted in
Healthy Indonesia Program (PIS-PK). Indicator drugs are TB-Hypertension drugs and ODGJ drugs. Data
Collection using Google form Results: From 39 Community Health Center (Puskesmas) the respondents who
followed : Sukabumi Regency 8 units, Sukabumi y 7 units; 3. Cianjur Regency 4 units; 4. Indramayu Regency 6
units; 5. Majalengka Regency 11 units; 6. Tasikmalaya Regency 3 units.
Keywords: JKN - PIS-PK- Puskesmas-Pharmaceutical Services and West Java
4
Oral Presentation [OP-006]
ANTICALCULI ACTIVITY OF APIGENIN AND CELERY (Apium graveolens, L) EXTRACT
IN ETHYLENE GLYCOL-AMMONIUM CHLORIDE INDUCED RATS
Sofi Nurmay Stiani1,3
, Fillah Muty Syahidah2, Hanindhiya Fikriani2, Anas Subarnas2 Taofik
Rusdiana1
1Department of Pharmaceutics and Pharmaceutical Technology, Padjadjaran University
2Department of pharmacology and Clinical pharmacy, Padjadjaran University 3Pharmacy Study Program, Salsabila Institute of Health Science, Serang Banten, Indonesia
Corresponding author: [email protected]
ABSTRACT
Kidney stones (Nephrolithiasis) is one of the kidney diseases in the form of stones which contain crystal and
organic matrix components. Nephrolithiasis is the most common disease of urinary tract disorders. Calcium stone
is the most important type of stone (80%) in the case of kidney stones. Celery (Apium graveolens L) is a plant rich
in flavonoids. Flavonoids are secondary metabolites that can shed calcium crystals. Apigenin is considered to be
one of the main flavonoids because of its presence and abundance in celery. In this study anticalkuli were tested
in vivo on mice by giving ethylene glycol 0.75% (v/v) and ammonium chloride 2% (w/v) orally for 7 days, then given
Apigenin doses 1.2, 2. 4, and 4.8 mg/kg of rat body weight and celery extract at the doses of 200, 400, 600 mg/kg
of rat bdy weight for 7 days. To analysed the anticalculi activity, calcium levels in levels in the kidneys and urine
of mice were determined by using atomic absorption spectroscopy. The results showed that both apigenin and
celery extracts had a tendency towards kidney stone decay activity, statistically the differences of calcium level
did show a significant value (p value<0.05) for Apigenin in kidney and urine but for celery extract only in urine.
Calcium level in kidney these differences did not show a significant value (p value>0.05).
Keywords: Nephrolithiasis, Apigenin, Celery extract, Anticalkuli, Calcium
5
Oral Presentation [OP-007]
THE EFFECT OF TRAINING ABOUT ANTI-HYPERTENSION TOWARD THE INCREASING KNOWLEDGE OF PHARMACEUTICAL SERVICES BY PUBLIC
HEALTH CENTER PHARMACIST IN PANDEGLANG REGENCY
Yusransyah1,2
, Eli Halimah2, Auliya A. Suwantika
2
1Department of Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia
2Pharmacy Study Program, Salsabila Institute of Health Science, Serang, Banten, Indonesia
Corresponding author: [email protected]
ABSTRACT
Pharmaceutical services by Pharmacist at Public Health Centers in Pandeglang are not considerably optimal. This
can be seen from the increasing of using anti-hypertensive drugs from year to year at Public Health Center, as the
biggest cause of death in Pandeglang Regency categorized to non-communicable diseases. This requires
pharmacists to optimize their roles farther by increasing their knowledge and capability through training. The study
aimed to determine the knowledge level and ability of Health Center Pharmacists in Pandeglang Regency and to
find out whether a significant influence could be emerged before and after training. This research was a pre-
experimental study with the design of one group given pretest, posttest and a questionnaire. Training was held
twice. The first training was about Communication Skill and the second one was about Anti-Hypertensive Therapy.
The results of this study were the first training indicated that the number of respondents who was not good or
respondents classified as poor knowledge was 42.86% before training, the number declined after training to 7.14%
and the increasing on the number of respondents classified as good enough or fair respondents, from 57.14%
before training to 92.86% after training. The average score was 66.57 after training higher than the score before
training was 55.36. Moreover, the second training indicated that the number of respondents who classified as poor
knowledge before training was 85.71%, the number declined after training to 14.29% and the increasing on the
number of respondents classified as knowledgeable or good respondents, from 0% before training to 14.29% after
training. The average score after training was 68.9 higher than the score before training was 42.5. Therefore, Ha
is accepted with a significant value of 0,000 (p = 0,000) both the first training and the second training, which means
less than 0.05. In conclusion, training can increase Pharmacist knowledge and ability to give pharmaceutical
services to patients about anti-hypertension.
Keywords: Anti-hypertension, Training, Knowledge Level, Pharmacist
6
Oral Presentation [OP-008]
KNOWLEDGE, ATTITUDES AND PRACTICES OF LUNG CANCER RISK FACTORS IN WEST BANDUNG SOCIETY
Mally Ghinan Sholih1), Dyah Aryani Perwitasari2), Rini Hendriani1), Hadyana Sukandar1), Melisa
Intan Barliana1), Auliya Suwantika1), Eky Septian1), Ajeng Diantini1)
1) Faculty of Pharmacy, University of Padjajaran, Bandung, Indonesia 2) Faculty of Pharmacy, University of Ahmad Dahlan, Yogyakarta, Indonesia
* email correspondence: [email protected]
ABSTRACT
Lung Cancer is one of the causes morbidity and mortality in the world and prevention needs to be done. This study
aimed to measure the knowledge, attitudes and practices of lung cancer risk factors in the West Bandung area.
The design of this study was quasy eksperimental. Measurement of respondents’s knowledge, attitudes and
practices was carried out by giving questionnaires. Questionnaires were given to respondents twice, before and
after giving education about risk factors for lung cancer. The data obtained then tested by comparison test and
correlation test. The results showed a significant difference in knowledge and attitudes p = 0,000 (p <0.05).
Meanwhile the practices was not sinificant p = 0.872 (p> 0.05). Correlation test shows that knowledge and attitudes
had a correlation p = 0,000 (p <0.05) to the pre-test and p = 0.23 (p<0.05) to post-test. This study shows that the
education plays a role to increasing knowledge and attitudes for risk factors of lung cancer.
Keywords: lung cancer, risk factor of lung cancer, KAP Study
7
Oral Presentation [OP-009]
ASSESSMENT OF HEALTH-RELATED QUALITY OF LIFE AMONG PATIENTS MANAGEMENT PROGRAM OF CHRONIC DISEASES TYPE 2 DIABETES MELLITUS
Fajriansyah1,2, Iskandarsyah A3, IM Puspitasari1,4, Keri Lestari1,4
1Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran,
Jatinangor-Sumedang, West Java, Indonesia;
2 School of Pharmacy Makassar, South Sulawesi, Indonesia;
3Department of Clinical Psychology, Faculty of Psychology, Universitas Padjadjaran, Jatinangor-
Sumedang, West Java, Indonesia;
4Centre of Excellence for Pharmaceutical Care Innovation, Universitas Padjadjaran, Jatinangor-
Sumedang, West Java, Indonesia.
* email correspondence: [email protected]
ABSTRACT
Type 2 Diabetes Mellitus (T2DM) has significant adverse effects on health-related quality of life. PROLANIS
program is a system of governance of health services and health education for social health insurance participants
who suffer from type 2 diabetes mellitus to achieve the optimal quality of life independently. This research aims to
measure quality of life in T2DM patient. The research was an analytical non-experimental study according to the
patient’s perspective using prospective data collection techniques. The subjects are Prolanis patient T2DM who
comply inclusion and exclusion criteria visited Antang, Batua, Jongaya, Tamalanrea, Primary Health Care
Makassar city, in August 2017 – January 2018. The data were collected by using the European Quality of Life 5
Dimensions 5 Levels (EQ-5D-5L) Indonesian questionnaire. Data analysis using EQ-5D preference weight for
each health state using Indonesian EQ-5D-5L Value Set. Furthermore, the percentage of the patients’ problems,
EQ-5D index (utility) and EQ-5D VAS were calculated. A total of 220 patients were included. Seventy two different
EQ-5D index values were described by the patients. Obtained 37 subjects (16.82%) had no problems. The EQ-
5D index value highest is 1,000 (n = 32, 14,54%) and EQ-5D index value lowest is -0,384 (n = 1, 0,45%). EQ-5D
index value has an average is 0.57 (SD 0,32) and EQ VAS value has an average is 72.19 (SD 14.73). This study
also confirmed that T2DM remains a critical predictor of health outcomes among patients. Results from this study
could be constructive in clinical practice, particularly in the early treatment of T2DM patients where improving
HRQoL is still possible.
Keywords: Type 2 Diabetes Mellitus, PROLANIS, Quality of life, EQ-5D-5L
8
Oral Presentation [OP-010]
FORMULASI MASKER GEL PEEL OFF EKSTRAK KULIT PISANG AMBON LUMUT
SEBAGAI ANTIOKSIDAN
Rossa Riau Wati1, Farid Perdana1, Framesti Frisma Sriarumtias1
1)Program Studi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Garut
e-mail address of corresponding author : [email protected]
ABSTRAK
Tanaman pisang merupakan salah satu jenis tanaman yang paling banyak terdapat di indonesia, pemanfaatan
buah pisang hanya terbatas pada buah nya saja, sedangkan kulit nya hanya menjadi limbah. Penelitian ini
bertujuan untuk memanfaatkan limbah kulit pisang ambon lumut (Musa Acuminata Colla) menjadi produk bernilai
jual dengan manfaat sebagai antioksidan. Metode ekstraksi yang digunakan yaitu dengan maserasi serta uji
antioksidan yang digunakan yaitu dengan metode DPPH. Formulasi yang digunakan yaitu Carbopol 940, PVA,
TEA, propilenglikol serta pengawet. Jumlah ekstrak yang digunakan mengacu pada nilai Ic50 dari ekstrak kulit
pisang ambon lumut yaitu 27,133 ppm. Hasil uji aktivitas antioksidan sediaan masker gel peel off , yang diperoleh
nilai IC50 yang paling tinggi adalah F3 dengan konsentrasi 0,135 % , dengan nilai IC 50, 37,91 ppm.
Kata Kunci : Antioksidan,Gel, Limbah, Kulit Pisang Ambon Lumut, Peel Off
9
Oral Presentation [OP-011]
EFEK BRIEF COUNSELING FARMASIS DAN PESAN PENGINGAT-MOTIVASI
RELIGIUSITAS TERHADAP KUALITAS HIDUP DAN HASIL TERAPI PADA PASIEN
DIABETES MELLITUS
Okta Muthia Sari1, Ginanjar Zukhruf S.2, Akrom2
1)Program Studi Farmasi, Fakultas MIPA, Universitas Lambung Mangkurat, Banjarbaru, Indonesia
2)Fakultas Farmasi, Universitas Ahmad Dahlan, Yogyakarta, Indonesia [email protected]
ABSTRAK
Diabetes mellitus merupakan penyakit yang bersifat jangka panjang sehingga dapat mempengaruhi kualitas hidup
penderitanya. Pengontrolan kadar gula darah sebagai outcome klinis serta peningkatan kualitas hidup adalah
tujuan dalam pengobatan diabetes mellitus. Penelitian bertujuan untuk mengetahui pengaruh brief counseling
farmasis disertai pesan pengingat-motivasi dengan pendekatan religiusitas terhadap outcome klinis kadar gula
darah puasa (GDP) dan kualitas hidup pasien diabetes mellitus rawat jalan di Puskesmas Jetis 1 Bantul,
Yogyakarta.Penelitian dilakukan dengan desain kuasi-eksperimental menggunakan pre-test – post-test control
group design. Pengumpulan data dilakukan secara prospektif pada pasien rawat jalan selama periode Mei sampai
Agustus 2018. Sejumlah 72 orang responden yang memenuhi kriteria inklusi dibagi dalam 2 kelompok, yaitu
kelompok perlakuan (36 pasien) dan kontrol (36 pasien). Pengumpulan data dilakukan dengan wawancara dan
pengisian kuesinoner 36-item Short Form (SF-36). Kadar GDP diambil dari rekam medis pasien. Analisis statistik
menggunakan uji Wilcoxon untuk nilai gula darah puasa dan uji t-dependen untuk nilai kualitas hidup.Hasil
penelitian menunjukkan konseling singkat farmasis (brief counseling) disertai pesan pengingat-motivasi dapat
menurunkan kadar GDP (∆ 34,85±63,36) dan meningkatkan kualitas hidup (∆ 13,73±9,22) secara signifikan
(p<0,05). Berdasarkan hasil penelitian ini dapat disimpulkan bahwa pemberian brief counseling farmasis disertai
pesan pengingat-motivasi dapat menurunkan kadar GDP dan meningkatkan kualitas hidup secara signifikan.
Kata kunci: Diabetes, Konseling, Pesan Singkat, Gula Darah Puasa, Kualitas Hidup
10
Oral Presentation [OP-012]
THE ETHANOLIC EXTRACT AND VARIOUS FRACTIONS OF MALAY APPLE (Syzygium
malaccense L. Merr & Perry.) LEAVES AS ANTIOBESITY AND THEIR IN VITRO INHIBITORY ACTIVITY ON PANCREATIC LIPASE ENZYME
Atun Qowiyyah1*, Elin Yulinah Sukandar1, Muhamad Insanu1
1School of Pharmacy, Institut Teknologi Bandung, Bandung, West Java, Indonesia
*corresponding author: [email protected]
ABSTRACT
Based on previous studies, it is known that some species of Syzygium genera have antiobesity effect and activity
as pancreatic lipase inhibitor which is one of the mechanism of action of antiobesity drugs. The objectives of this
study were to investigate the antiobesity effect of ethanolic extract and various fractions of malay apple leaves and
their in vitro inhibitory activity on pancreatic lipase enzyme. The antiobesity study was conducted on Wistar female
rats induced by high fat-high carbohydrate diet for 6 weeks followed by 3 weeks treatment using ethanolic extract
and various fractions (n-hexane, ethyl acetate, water, and methanol) of malay apple leaves. Induction was
continued during the treatment.The inhibitory activity of pancreatic lipase was conducted in vitro using colorimetric
method. After induction, the percentage of body weight increase to 150.0-335.9% without trigger
hypertriglyceridemia and hypercholesterolemia. The ethanolic extract and various fractions of malay apple leaves
had antiobesity effect by inhibiting the body weight gain. The highest effect was showed by methanolic fraction
with 153.7 ± 35.5 % inhibition. They did not affect fecal, liver, and abdominal fat index, cholesterol and triglyceride
blood level. All of samples could inhibit pancreatic lipase activity. The highest inhibitory activity was showed by
ethanolic extract of malay apple compared to orlistat as positive control. The IC 50 of both samples were 3.46 and
3.31 μg/mL respectively. The ethanolic extracts and various fractions of malay apple leaves had antiobesity effect
and one of the mechanism action by inhibiting pancreatic lipase activity.
Keywords: Obesity, Syzygium Malaccense, Ethanolic Extract, Pancreatic Lipase Enzyme
11
Oral Presentation [OP-013]
PREDICTING THE EXCESS SOLUBILITY INCLUSION COMPLEX OF 𝜷-CYCLODEXTRIN
WITH α-MANGOSTIN AND ITS DERIVATIVES VIA MOLECULAR DYNAMIC
PREDIKSI SOLUBILITAS KOMPLEKS INKLUSI β-SIKLODEKSTRIN DENGAN α-MANGOSTIN DAN TURUNANNYA MELALUI SIMULASI DINAMIKA MOLEKUL
Dwina Ramadhani Pomalingo, Muchtaridi Muchtaridi*
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Padjadjaran
University, 45363 Sumedang, Indonesia. Telephone / Fax: (022) 7796200
e-mail address of corresponding author: [email protected]
ABSTRACT
α-mangostin merupakan senyawa xanthone yang berwarna kuning yang diekstrak dari kulit Manggis (Garcinia
mangostana L) yang telah terbukti memiliki aktifitas anti-radang, antijamur, antimikroba serta anti-kanker.
Beberapa pendekatan dilakukan dalam pengembangan obat, salah satunya adalah dengan memodifikasi suatu
senyawa dengan pendekatan model komputasi, Penelitian-penelitian kimia dengan menggunakan komputer
sebagai alat bantu terus dilakukan dan dikembangkan. Pembentukan kompleks inklusi merupakan salah satu cara
untuk meningkatkan kelarutan zat aktif yang memiliki kelarutan yang rendah dalam air, dengan demikian
diharapkan ketika kelarutannya meningkat maka laju disolusi juga akan meningkat. Metode simulasi dinamika
molekul memerlukan struktur protein sebagai masukan (input). Reseptor Human Estrogen Alpha telah tersedia
di database protein data bank (PDB) dengan kode 3ERT, oleh karena itu pada penelitian ini dilakukan metode
simulasi dinamika molekul untuk mempelajari perbedaan perilaku struktural Alpha mangostin dan Sikolodekstrin
sebagai senyawa anti kanker payudara.
Keywords: Dinamika Molekul, Kompleks Inlusi, β-siklodekstrin, α-mangostin,
12
Oral Presentation [OP-014]
THE POTENTIAL OF ANTIHYPERLIPIDEMIC DIETARY FIBER IN CELERY (Apium
graveolens L.) AQUEOUS EXTRACT RESIDUE BY USING IN VIVO METHOD ON LIPID
PROFILE PARAMETERS Nopi Rantika1*, Taofik Rusdiana1, Anas Subarnas2
1)Department of Pharmacy, Faculty of Pharmacy, Padjadjaran University, Sumedang, Indonesia,
45363 2)Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Padjadjaran University,
Indonesia 45363
ABSTRACT
Hyperlipidemia is a condition characterized by an increase in lipid profile or lipoproteins in blood. The high level of
lipids in the blood is a risk indicator of several diseases including atherosclerosis, dyslipidemia, and coronary artery
disease. It is also often categorized as the sudden death syndrome1. Several studies have shown that the
consumption of high dietary fiber can significantly reduce total cholesterol (TC) and low density lipoprotein (LDL)
cholesterol. One of the procedures is to induce a partial block of the enterohepatic circulation with intestinal
bacteria-fermented dietary fiber products2. This study aims to determine the dietary fiber activity of the celery
(Apium graveolens L.) aqueous extract residue on lipid profiles by using vivo method on white Wistar rats (Rattus
novergicus) induced by hyperlipidemia. Results indicated that the celery aqueous extract residue has a high dietary
fiber of 71.55%. The total, insoluble and soluble dietary fibers have significant antihyperlipidemic activity (p> 0.05).
The fibers can decrease the total cholesterol level, triglyceride level and LDL-cholesterol level, as well as increase
the high density lipoprotein (HDL)-cholesterol level. The high activity can be seen in the group of rats induced by
soluble dietary fiber.
Keywords: Anti-Hyperlipidemia, Apium Graveolens, Dietary Fiber
13
Oral Presentation [OP-015]
TINJAUAN SISTEMATIK: EVALUASI EKONOMI VAKSINASI HPV DI DAERAH YANG DITARGETKAN
Dedy Frianto1),
1)Fakultas Farmasi Universitas Padjadjaran
e-mail address of corresponding author : [email protected]
ABSTRAK
Kanker serviks menduduki posisi 4 besar dalam kejadian kanker yang paling banyak dialami oleh kaum wanita di
dunia dengan angka kematian terus meningkat pada tahun 2012 sampai dengan tahun 2018. Efektivitas
penggunaan vaksin HPV telah banyak dibuktikan dari studi diberbagai negara, tetapi banyak negara yang belum
menerapkan program vaksinasi HPV menjadi program vaksinasi lokal maupun nasional, dikarenakan faktor
ekonomi (budget) yang dimiliki oleh negara terbatas. Perbedaan PDB nasional dengan PDB Regional menjadi
penyebab program vaksinasi sulit diimplementasikan, selain itu faktor sosial budaya dan agama juga
mempengaruhi, pada penelitian ini dilakukan suatu tinjauan sistematik tentang evaluasi ekonomi Vaksinasi HPV
di daerah yang ditargetkan. Jika implementasi nasional tidak dapat dilaksanakan implementasi regional dapat
dijadikan alternatif. Metode dalam penelitian ini dengan melakukan review jurnal secara sistematik dari 2 data
base Proquest dan Pubmed dan dilakukan sksrining artikel yang sesuai dengan tema sudah ditentukan. Hasilnya
diperoleh 5 artikel yang memenuhi kriteria yang sudah ditentukan yaitu penelitian yang dilakukan di india, Laos,
Vietnam, Barazil dan Tanzania dengan kesimpulan umum harga vaksin HPV berkisar 4.6 sampai 7.8 USD.
Pemberian vaksin HPV menunjukan hasil cost effective diberikan pada anak perempuan usia 9 - 12 tahun dan
mencegah angka kejadian kanker serviks 35% -75% dengan kondisi masing-masing daerah.
Keywords: Kanker serviks, HPV, Vaksinasi, Evaluasi Ekonomi
14
Oral Presentation [OP-016]
THE EFFECTIVITY OF ETHANOL EXTRACT OF CORTEX FRUCTUS DUKU (Lansium
domesticum Corr.) AS A SKIN LIGHTENING CREAM
Lusi Nurdianti1,2 , Anindita Trikusumah1, Winda Trisna Wulandari1, Ayi Nurdin1
1Pharmacy Departement, Bakti Tunas Husada Institute of Health Science, Tasikmalaya
2Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Padjadjaran University
ABSTRACT
Lightening cream is a medical cosmetics preparation that is used to inhibit melanin pigment formation so that it
can whiten the skin. The active substance used is ethanolic extract of cortex fructus duku (Lansium domesticum
Corr.). The purpose of this study was to determine the effect extracts of cortex fructus duku waste (Lansium
domesticum Corr. on the results of the evaluation of the preparation, the level of irritation to rabbits, and an
increase in the brightness of human skin. In this study used a concentration variation of 10,15 and 20% cortex
fructus duku ethanol extract. The results showed that all formulas met the requirements of organoleptic test, pH,
homogeneity, dispersion, and centrifugation. The irritation test with the Draize method obtained all non-irritating
formulas while the results of the brightness test on the skin obtained in formula 3 were the best in inhibiting melanin
pigment formation.
Keywords: Lightening Cream, Cortex Fructus Duku, Melanin
15
Oral Presentation [OP-017]
COMMON SENSE SELF REGULATORY MODEL (CSSRM) IN DIABETIC PATIENTS
USING ANTIHYPERTENSION AND/OR ANTIHYPERLIPIDEMIA IN PANEMBAHAN SENOPATI GENERAL HOSPITAL BANTUL YOGYAKARTA
Muthia Dewi M. A1, Dyah Aryani Perwitasari1, Rizky Abdullah2, Sofa Dewi Alfian3
1Fakultas Farmasi, Universitas Ahmad Dahlan, Yogyakarta 2,3Fakultas Farmasi, Universitas Padjajaran, Bandung
Email: [email protected]
ABSTRACT
Background: Indonesia is the sixth most DM country in the world with 10.3 million people suffering from DM.
Diabetes mellitus is a major factor in the occurrence of cardiovascular disease. One of them is the increase in
the number of DM patients caused by the patient's non-compliance with the treatment of the disease.
Objective: To determine the relationship between medication beliefs and adherence and to know the
relationship of medication beliefs to quality of life and therapeutic outcomes in the treatment of patients with
Diabetes with hypertension and/or hyperlipidemia in Panembahan Senopati General Hospital Bantul
Yogyakarta.
Research Methods: Design of research used a cross sectional approach. The study subjects fulfilled the
inclusion requirements of 50 patients. The research instrument used was a questionnaire, including Brief
illness perception questionnaire (BIPQ), Diabetic distress scale (DDS), Medication Adherence Report Scale
(MARS), Beliefs about Medications Questionnaire (BMQ) and European quality of life-5 dimensions (EQ- 5D).
Analysis of the data used the analysis of Structural Equation Modeling (SEM) PLS to determine the relationship
between variables and linear regression test, Man Whitney and Kruskal Wallis to determine the relationship
between the characteristics of the research subjects and the variables used.
Results and Discussion: Obtained 50 respondents who met the inclusion criteria. Consisting of 11 patients
from the diabetes group with hypertension and hyperlipidemia and 39 patients from the diabetes group with
hypertension. Based on the results of an analysis of the characteristics of patients obtained by age, it affects
medication beliefs, where patients are concerned about the adverse effects of long-term drug consumption.
The results of the analysis of the relationship between variables indicate that stress is a factor that has been
shown to affect quality of life.
Conclusions: Medication beliefs affect adherence in DM patients with hypertension and hyperlipidemia.
Compliance does not affect the quality of life and outcome of therapy in DM patients with hypertension and /
or hyperlipidemia. Medication beliefs, perceptions and characteristics do not affect directly or indirectly
(through adherence) to quality of life and outcome of therapy. While distress affects the quality of life in DM
patients with hypertension.
Keywords: Diabetes, Medication Beliefs, Common Sense Sel Regulatory Model (CSSRM)
16
Oral Presentation [OP-018]
PENETAPAN KADAR ASPARTAM DAN SAKARIN PADA JAMU INSTAN UNTUK ANAK DENGAN METODE KCKT
Winasih Rachmawati1,2, Idar1, Sita Zuliani Agustin1
1) Sekolah Tinggi Farmasi Bandung, Jl. Soekarno Hatta No.754 Cibiru Bandung
2) Fakultas Farmasi, Universitas Padjadjaran, Jatinangor, Sumedang
Email: [email protected]
ABSTRAK
Budaya mengkonsumsi jamu masih merupakan salah satu upaya pencegahan berbagai masalah kesehatan
di Indonesia. Jamu instan merupakan salah satu produk yang praktis untuk dikonsumsi sehingga lebih disukai
oleh anak-anak. Penambahan pemanis pada jamu instan untuk anak diperlukan untuk mengurangi rasa pahit
dari bahan alam yang terkandung di dalamnya. Aspartam dan sakarin merupakan pemanis sintetis yang dapat
ditambahkan dengan batasan tertentu. Tujuannya dari penelitian ini adalah untuk mengetahui kadar aspartam
dan sakarin yang terkandung di dalam jamu instan anak. Pada penelitian ini digunakan metode KCKT dengan
kolom C18, fase gerak bufer posfat pH 4,93:asetonitril (20:80 v/v), laju alir 0,5 mL/menit dan detektor UV pada
225 dan 267 nm. Sistem ini telah memenuhi persyaratan validasi linearitas dengan nilai koefisien korelasi (r)
aspartam dan sakarin adalah 0,9984 dan 0,9987. Batas deteksi aspartam dan sakarin adalah 3,34 dan 2,99
µg/ml, sedangkan batas kuantitasi aspartam dan sakarin adalah 11,13 dan 9,98 µg/ml. Presisi ditunjukkan
dengan nilai simpangan baku relatif (SBR) aspartam dan sakarin adalah 2,09 dan 1,95%. Hasil akurasi
ditunjukkan dengan nilai persen perolehan kembali untuk aspartam 110-115%, sedangkan untuk sakarin pada
rentang 94-98%. Dari hasil penelitian diketahui bahwa ketiga sampel jamu instan anak mengandung sakarin.
Sampel 1 dan 2 tidak memenuhi persyaratan batas maksimum sakarin dalam obat tradisonal menurut BPOM
yaitu 1200 mg/kg produk. Sedangkan pemanis aspartam tidak teridentifikasi di dalam semua sampel jamu
instan anak.
Kata Kunci : Aspartam, Sakarin, Jamu Instan, KCKT
17
Oral Presentation [OP-019]
DOSAGE AND DURATION OF METHYLPREDNISOLONE THERAPY AFFECT THE
OCCURENCE OF CUSHING HABITUS IN SYSTEMIC LUPUS ERYTHEMATOSUS
PATIENTS
Didi Permana1, Melisa I Barliana2,3 , Laniyati Hamijoyo4 1) Master Program in Clinical Pharmacy, Faculty of Pharmacy, University of Padjadjaran, Indonesia
2) Department of Biological Pharmacy, Biotechnology Pharmacy Laboratory, Faculty of Pharmacy, University of Padjadjaran
3) Center of Excellence in Higher Education for Pharmaceutical Care Innovation, University of Padjadjaran, Indonesia
4) Rheumatology Division, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran / Hasan Sadikin Hospital, Indonesia
e-mail address of corresponding author : [email protected]
ABSTRACT Systemic Lupus Erythematosus (SLE) is a multisystem disease with very diverse developments. Corticosteroid
is most used for the treatment of SLE as anti-inflammatory and immunosuppressant, but its use in long term
and high dose can cause the side effects such as cushing habitus. This study aimed to analyze the risk factors
of cushing habitus occurence in SLE patients, consisting of pulse dose, duration of therapy, daily dose, and
total dose of methylprednisolone. A case control study was conducted with the sample population were SLE
patients treated at Rheumatology out patients clinic at Hasan Sadikin Hospital in Bandung. Each of the case
and control groups consisted of 40 patients. Chi square test was used to test the relationship between
independent variables followed by linear logistic regression analysis to determine the influence of the most
influential variable in causing cushing habitus. The results of this study showed that the use of total dose
metilprednisolon >8040 mg has a significant effect on the incidence of cushing habitus (p = 0.029, OR 3.55).
In addition, daily dose of methylprednisolone >8.4 mg has a significant effect on cushing habitus with value (p
= 0.012 OR = 2.98).
Keywords: Total Dose, Daily Dose, Pulse Dose, Cushing Habitus.
18
Oral Presentation [OP-020]
MOLECULAR DOCKING SENYAWA CHALCONE DAN TURUNANNYA TERHADAP SEL KANKER PAYUDARA (T47D)
Richa Mardianingrum1,2, Aas Nuraisah1, Yunia Sarwatiningsih1, Ruswanto3
1)Program Studi Farmasi, Universitas Perjuangan, Jl. Peta No.177, Kahuripan, Kec Tawang
Tasikmalaya, Jawa Barat 46115 2)Fakultas Farmasi, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM 21, Hegarmanah,
Kec. Jatinangor, Kabupaten Sumedang, Jawa Barat 45363 3)Program Studi Farmasi, Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada, Jl. Cilolohan No.
36, Kahuripan, Kec Tawang Tasikmalaya, Jawa Barat 46115
Email: [email protected]
ABSTRAK
Kanker payudara merupakan salah satu jenis kanker dengan angka kematian yang cukup tinggi. Pada
penelitian sebelumnya telah disintesis turunan senyawa chalcone dan telah terbukti secara in vitro aktif pada
sel kanker payudara (T47D). Tujuan dari penelitian ini adalah mengetahui interaksi antara turunan senyawa
chalcone yang disintesis dengan reseptor pada sel kanker payudara secara in silico. Penelitian dilakukan
menggunakan metode molecular docking dengan softwere AutoDock 1.5.6. Struktur chalcone dan
turunannya yang telah dipreparasi menggunakan Marvin Sketch, kemudian dilakukan proses docking
terhadap residu pada ER-α (3ERT). Hasil docking menunjukkan senyawa chalcone 3 memiliki potensi
sebagai anti kanker payudara (T47D) dengan nilai ΔG dan Ki (μM) sebesar -7.05 kcal/mol dan 6.74 μM.
Chalcone 3 membentuk ikatan hidrogen dengan asam amino Ala350 dan ikatan hidrofobik dengan asam
amino Leu539, Leu536, Glu353, Leu387, Asp351, Tyr526. Senyawa chalcone 3 memenuhi syarat dari aturan
Lipinski’s rule of five, diabsorpsi dengan baik pada usus, ikatan protein plasmanya terikat kuat tetapi tidak
terlalu banyak direabsorpsi di ginjal. Adanya gugus hidroksi dan gugus Cl pada chalcone 3 dapat menaikkan
aktivitas senyawa. Dapat disimpulkan bahwa chalcone 3 memiliki potensi paling efektif untuk obat anti kanker
payudara (T47D) melalui penghambatan pada ER-α.
Kata kunci: Molekular Docking, Chalcone, Kanker Payudara (T47D), ER-α
19
Oral Presentation [OP-021]
OVERVIEW OF CURRENT AND FUTURE TARGETS OF BREAST CANCER
MEDICINES
Eri Amalia1*([email protected]), Ajeng Diantini1([email protected]), Anas Subarnas1
([email protected]) 1Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Padjadjaran
University, Jl. Raya Bandung-Sumedang km.21 Jatinangor - 45363, Indonesia.
ABSTRACT
Nowadays, many drugs are available for the treatment of breast cancer disease. Particular drugs are
applied depending on the types of cancer cells known as Luminal-A, Luminal-B, Basal-like, HER2+, and
Triple Negative Breast Cancer. This study summarizes those specific actions, also reveal one of new
validated target therapy which beneficial for drug discovery of breast cancer medicine.
Method: We did an analysis of breast cancer medicines from first approved by the US FDA in 1953 until
May 2019. The drugs were identified in term of its mechanism of action and binding site to inhibit cancer
cell proliferation.
Result: To date, 33 drugs had been approved by the US FDA for breast cancer treatment. Thirteen drugs
are known as chemotherapy drugs, with the mechanism of action as an alkylating agent, Dihydrofolate
reductase– thymidylate synthetase inhibitor, and microtubulin inhibitor. Twelve drugs are known as
targeted therapy including three monoclonal antibody targeting HER2/ErbB2 and a monoclonal antibody
inhibit the interaction of programmed cell death protein-1/programmed cell death-ligand 1, and eight small
molecule inhibitors are targeting intracellular enzyme including a tyrosine kinase, Cyclin-Dependent
Kinase 4/6, Poly(Adenosine Diphosphate-Ribose) Polymerase-1. Eight drugs are hormonal therapy to
reduced estrogen level and block the growth of cancer cell.
Conclusion: We found that the roadmap of the breast cancer treatment approach is changing from
chemotherapy into targeted therapy both as small molecule inhibitor and monoclonal antibody.
Meanwhile, hormonal treatment remains useful for cancer women with hormonal dysregulation.
Alternatively, the discovery of breast cancer drugs targeting Dihydroorotate dehydrogenase is promising
to be developed in the future.
Keywords: Breast Cancer, Dihydroorotate Dehydrogenase, CDK4/6, PARP, HER2/Erbb2, Tyrosine Kinase, Mtor, Aromatase, Monoclonal Antibody, PD-L1.
20
Oral Presentation [OP-022]
ANTIOXIDANT ACTIVITY OF CRUDE BROMELAIN OF PINEAPPLE (Ananas comosus
(L.) Merr) CROWN
Nyi Mekar Saptarini1)*, Driyanti Rahayu, 1) Irma Erika Herawati2)
1) Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl Raya Bandung Sumedang Km 21, West Java, Indonesia, 45363
2) Department of Pharmacy, Faculty of Mathematic and Natural Sciences, Al Ghifari University, Jl Cisaranten Kulon 140, West Java, Indonesia, 40293
* e-mail address of corresponding author: [email protected]
ABSTRACT
Antioxidants are chemical compounds that can donor one or more electrons to free radicals to prevent
degenerative diseases. The crown of pineapple (Ananas comosus (L.) Merr.) contain bromelain, i.e. proteolytic
enzyme, which can act as an antioxidant. The purpose of this study was to determine the antioxidant activity
of crude bromelain from pineapple crowns. Antioxidant activity was determined by the method of 2,2-diphenyl-
1-picryhydrazyl (DPPH) with vitamin C as a standard. The pineapple crown was produced 0.26% of dried crude
bromelain with total protein content was 1.32 ± 0.42% and IC50 value was 3624 ppm of crude bromelain which
equivalent to 47.84 ppm of total protein. We concluded that bromelain crude was strong antioxidants.
Keywords: DPPH, IC50 Value, Strong Antioxidants
21
Oral Presentation [OP-023]
LARD ANALYSIS IN SAUSAGE USING FTIR SPECTROPHOTOMETER COMBINED
WITH CHEMOMETRICS
Any Guntarti1, Satriyo Wisnu Prihandoko1
1Fakultas Farmasi Universitas Ahmad Dahlan
Kampus III: Jl. Prof. Dr. Soepomo, Janturan, Umbulharjo, Yogyakarta 55164
Email: [email protected]
ABSTRACT
Halal food product was very important to taken into consideration by consumer, one of them is sausage. Meat
is commonly used in producing sausages, especially beef. But because expensive, producen usually mixing
the indegrients with other cheaper meats, for example pig. This study was aimed to analyze the differences in
the spectral profile of lard and beef in the sausages using FTIR. Lard and beef tallow was extracted using
Soxhlet at ± 70°C for 6 hours with n-hexane. After ectracted then evaporated and obtained fats was stored in
appendorf then it was analyzed using FTIR spectrophotometer. The results combined with chemometrics,
Partial Least Square (PLS) for the quantitative analysis and Principal Component Analysis (PCA) for
classification. PLS and PCA analysis was performed on 1200-1000 cm-1. The results of the analysis of PLS
provides the linear regression equation y = 0,921x + 4.623 with R2 0.985 and RMSEC 2.094%. External
validation, RMSEP obtained 4.77% and internal validation RMSECV obtained 5.12%. PCA analysis results
showed the classification of different quadrants between 100% pork sausage with 100% beef sausage. The
conclusion of this research was FTIR method combined with chemometrics can be applied to identify the
presence of pork in the sausage.
Keywords: Beef Fat, FTIR Spectrophotometer, Lard, PCA, PLS
22
Oral Presentation [OP-024]
RADIO-HPLC IMPURITIES ANALYSIS OF 131I-ALPHA MANGOSTIN AND 99mTC-
ALPHA MANGOSTIN AS POTENTIAL BREAST CANCER RADIOTRACER
Luthfi Utami Setyawati1), Risda Rahmi Islamiaty1), Kevin Reinard Lie1), Wiwit Nurhidayah1), Eva Maria Widyasari2), Rizky Juwita Sugiharti2), Maula Eka Sriyani2), Muchtaridi Muchtaridi1)
1) Departement of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy,
Padjadjaran University, Jl. Bandung-Sumedang KM.21, Jatinangor, West Java, Indonesia, 45363
2) Center for Applied Nuclear Science and Technology of National Nuclear Agency (PSTNT BATAN), Jl. Tamansari No.71, Bandung, West Java, Indonesia, 40132
e-mail address of corresponding author : [email protected]
ABSTRACT
The Globocan database 2018 showed that breast cancer is still the most common cancer affects women and
it is also the main cause of cancer death in woman. Radiolabeling α-mangostin with Technetium-99m and
Iodin-131 has functions as breast cancer radiotracer and radiotherapy. The aim of this study was to validate
the methodology for analysis of chemical impurities of 131I-α mangostin and 99mTc-α mangostin. Chemical
impurities can affect efficiency of radiopharmaceuticals. The analysis was conducted by radio-HPLC with C18
column, methanol:water (90:10) as mobile phase, with UV (243 nm) tandem radioactive detector (Gabi Star).
The analytical method was met the ICH Q2 (R1) validation criteria, thus it could be applied in the quality control
of radiopharmaceuticals. Surprisingly, the results of labelling identification showed that 131I-α mangostin had
been successfully formed while the HPLC system used for 99mTc-α-mangostin identification showed that the
desired complex has not yet been formed perfectly because it still has chemicals impurities.
Keywords : Breast Cancer, α-Mangostin, Validation Method, Chemical Impurities, Radio-HPLC
23
Oral Presentation [OP-025]
RADIOLABELLlNG OF ALPHA MANGOSTIN USING IODINE-131 AS NEW BREAST CANCER DIAGNOSTIC AGENT
Wiwit Nurhidayah1), Luthfi Utami Setyawati1), Risda Rahmi Islamiaty1), Eva Maria Widyasari2), Rizky Juwita Sugiharti2), Maula Eka Sriyani2), Muchtaridi Muchtaridi1)
1) Departement of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy,
Padjadjaran University, Jl. Bandung-Sumedang KM.21, Jatinangor, West Java, Indonesia, 45363
2) Center for Applied Nuclear Science and Technology of National Nuclear Agency (PSTNT BATAN), Jl. Tamansari No.71, Bandung, West Java, Indonesia, 40132
e-mail address of corresponding author : [email protected]
ABSTRACT
Alpha mangostin is a xanthone compound that was isolated from mangosteen peel. Base on various study,
alpha mangostin has anticancer activities, especially for breast cancer. Therefore, the radiolabelled of alpha
mangostin was developed for alternative method for detecting breast cancer. Radioisotope iodine-131 was
used because it has optimal energy and stable bonds with the labeled compound. The aim of this study was
to measure optimum conditions to synthesis radiolabelled compound 131I-α-mangostin with high radiochemical
purity. The radiolabelling method was used chloramine T method. Some parameters were optimized including
pH, amount of chloramine T and Na2S2O5, amount of alpha mangostin and incubation time. Radiochemical
purity was determined by electrophoresis and thin layer chromatography, then was measured by Single
Channel Analyzer. The result showed that optimum conditions at the pH 10, amount of chloramine T 0.125
mg, Na2S2O5 0.244 mg, alpha mangostin 0.625 mg dan incubation time 50 minutes with radiochemical purity
90.63 ± 0.44%.
Keywords: Alpha Mangostin, Iodine-131, Breast Cancer, Radiochemical Purity.
24
Oral Presentation [OP-026]
OPTIMIZATION CONDITION FOR RADIOLABELING ALPHA MANGOSTIN WITH
TECHNETIUM-99M FOR BREAST CANCER RADIODIAGNOSTIC
Risda Rahmi Islamiaty1), Luthfi Utami Setyawati1), Kevin Reinard Lie1), Wiwit Nurhidayah1), Rizky Juwita Sugiharti2) , Eva Maria Widyasari2), Maula Eka Sriyani2), Muchtaridi Muchtaridi1)
1) Departement of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy,
Padjadjaran University, Jl. Bandung-Sumedang KM.21, Jatinangor, West Java, Indonesia, 45363
2) Center for Applied Nuclear Science and Technology of National Nuclear Agency (PSTNT BATAN), Jl. Tamansari No.71, Bandung, West Java, Indonesia, 40132
e-mail address of corresponding author : [email protected]
ABSTRACT
Breast cancer is leading cancer that affects women until now. There are several methods for diagnosing breast
cancer, but there are still have shortcomings. Therefore, a new specific and safe method is needed to diagnose
breast cancer. Radiolabeled compound combined by natural compound could be an option for early diagnosis
of breast cancer. Alpha mangostin which has anti breast cancer activity can be selected to be labeled with
Technetium-99m. The aim of this study was to obtain the optimum radiolabeling condition of Tc-99m labeled
α-mangostin as a breast cancer radiodiagnostic. The radiolabeling was conducted by using indirect labeling
method by optimized some parameters. The results showed that the optimum formulation for 99mTc-α-
mangostin consisted of 300 µg α mangostin, 90 µg SnCl2.2H2O, 150 µg EDTA at pH 9 with the addition of 0.3
mCi Tc-99m and 5 minutes for incubation time in room temperature. The radiochemical yield was confirmed
by thin layer chromatography, paper chromatography and electrophoresis. Unfortunately, the optimum formula
just gave the purity of 70.6%±2.87% that did not met the USP specification for radiolabeled compound.
Keywords: Breast Cancer, Radiolabeled Compound, Alpha Mangostin, Diagnosis, Optimum Condition
25
Oral Presentation [OP-027]
STRUCTURAL DESAIN-BASED IN SILICO STUDY OF α-MANGOSTIN AS ACTIVE
LIGAND α-ESTROGEN RECEPTOR
DESAIN STRUKTUR BERBASIS STUDY IN SILICO DARI α-MANGOSTIN SEBAGAI LIGAN AKTIF RESEPTOR ESTROGEN-α
Muhammad Fauzi, Muchtaridi Muchtaridi*
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Padjadjaran University, 45363 Sumedang, Indonesia. Telephone / Fax: (022) 7796200
e-mail address of corresponding author : [email protected]
ABSTRACT
Sekitar 23% kasus kanker yang terjadi pada wanita adalah kanker payudara. Salah satu titik tangkap
pengobatan kanker payudara adalah dengan menghambat aktivitas estrogen pada reseptor estrogen alfa
(ERα). Sampai saat ini tamoksifen masih menjadi lini pertama dalam pengobatan kanker payudara namun
memiliki efek samping berupa kanker endometrium dan tromboemboli. α-mangostin merupakan derivat utama
xanton dari kulit buah manggis (Garcinia mangostana L) yang memiliki aktivitas anti ploriferatif sel kanker
payudara MCF-7, tetapi memiliki kelarutan yang buruk. Pada penelitian ini dilakukan desain modifikasi untuk
mendapatkan modifikasi struktruk α-mangostin sebagai ligan aktif ERα dengan uji in silico menggunakan
penambatan yang telah divalidasi. Desain modifikasi terbaik divisualisasii posenya pada kantung ikatan ERα
dan dilakukan analisis untuk menentukan rute sintesisnya. Hasil penelitian yang dilakukan terdapat enam
desain modifikasi struktur α-mangostin yang dapat bertindak sebagai ligan aktif terhadap ERα yaitu desain
modifikasi struktur MF1, MF2, MF3, MF4, MF5 dan MF6. Berdasarkan hasill analisa tersebut senyawa baru
yang memiliki kelarutan paling baik adalah MF6. Mekanisme sintesis yang diusulkan untuk memperoleh
senyawa tersebut menggunakan 3 jalur reaksi yaitu asetilasi, ritter dan hidrolisis dimana dimulai dengan
modifikasi MF3, MF5 dan MF6.
Keywords: In Silico, Modifikasi α-Mangostin, Kanker Payudara, Reseptor Estrogen-α
26
Oral Presentation [OP-028]
IMPROVEMENT DISSOLUTION RATE AND BIOAVAILABILITY SIMVASTATIN
TABLET
Dolih Gozali, Taofik Rusdiana and Firman Gustaman
Department of Pharmaceutics and Technology of Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, Indonesia
E-mail Correspondence to: [email protected]
ABSTRACT
Simvastatin is a statin drug that used to reduce cholesterol levels via inhibition of the 3-hydroxy-3-
methylglutaryl-coenzyme A (HMG-CoA) reductase. Simvastatin is classified as a Biopharmaceutics
Classification System (BCS) Class-II compound with a poor aqueous solubility and high permeability. The low
bioavailability in simvastatin is due to the influence of P-glycoprotein which is the transforter effluk that plays a
role in drug absorption. To increase the bioavailability of simvastatin, it is necessary to add a surfactant, a
surfactant to be added to the simvastatin tablet production of Cremophor RH 40 which serves as a solubilizer
and enhancer.The purpose of the study is to determine the effect of Cremophor RH 40 to the dissolution rate
and bioavailability improvement of simvastatin tablet. The experiment was consist of four formula with different
concentration of Cremophor RH 40 that were 0%, 0,5%, 1% and 1,5%. Methods. The determination of
concentration of dissolution test results was use a UV-Vis spectrophotometer at a wavelength of 238 nm.
Tolerance within 30 minutes should dissolve not less than 75% (Q) of simvastatin. The formulas with the best
value were tested in vivo to the animals. The test animals were use male white rats wistar rattus norvegicus
which was divided into 2 groups, each group consisted of 8 rats. The simvastatin tablets of each suspended
formula using Na-CMC were given for 7 days with the same of time and place. On the 7th day, exactly at the
tp max point, the rats blood were taken as Cmax∞ and then the plasma was analyzed with HPLC.. The
dissolution test results of simvastatin tablet formulas with different concentrations of Cremophor RH 40
indicated that formula 2 and formula 3 gave significant differences (p <0.05) compared to formula 1. In addition,
formula 4 did not give significant difference (p <0, 05) than formula 1. Addition of Cremophor RH 40 of 1% in
formula 3 has the largest simvastatin dissolution rate. It can be interpreted that the addition of Cremophor RH
40 on the manufacture of simvastatin tablets can increase dissolution rate. The analyze of HPLC on Formula
3 (addition of Cremophor RH 40 of 1%) which is the best dissolution result and formula 1 (without addition of
Cremophor RH 40) showed that formula 1 and formula 3 have significant differences between the average of
maximum concentration 1 (0.1211 ppm) compared to the maximum concentration of formula 3 (0.3968 ppm).
It can be concluded that the addition of Cremophor RH 40 as a surfactant can increase dissolution rate and
bioavilability of simvastatin tablet.
Keywords: Simvastatin, Cremophor RH 40, Dissolution, HPLC
27
Oral Presentation [OP-029]
THE EFFECT OF BENGLE (Zingiber cassumunar Roxb.) RHIZOME ETHANOL
EXTRACT ON SUPEROSIDA DISMUTASE (SOD) ENZYM ACTIVITY IN
HYPERLIPIDEMIC RATS
Novita Sari1), Nurkhasanah2), Nanik Sulistyani3)
1) Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta, Indonesia 2) Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta, Indonesia
E-mail address of corresponding outhor : [email protected]
ABSTRACT
Hyperlipidemic increase the formation of Reactive Oxygen Species (ROS), because it causes endothelium
cell dysfunction. The increase of ROS must be balanced with total antioxidant, so that additional antioxidants
from outside are needed. Rhizome Bengle has been studied to have phenolic compounds, flavonoids,
curcuminoids and essential oils which are potential antioxidant. The study to determine antioxidant activity of
Bengle rhizome ethanol extract on superosida dismutase (SOD) enzyme in hyperlipidemic rats. Twenty five
male wistar rats were devided into five group: normal group, negative control group induced by high fat diet
(HFD) for 14 day, the treatment groups induced HFD for 14 day and given 100, 200 and 400 mg/kg BW of
Bengle rhizome ethanol extract. The liver rat was used to measure the activation of SOD enzyme using Assay
Kit. The treatment of Bengle rhizome ethanol extract can increase SOD enzyme activity in hyperlipidemic rats
compred to control. Extract Z. cassumunar Roxb. can increase antioxidant in vivo.
Keywords: Bengle rhizome, antioxidant, SOD
28
Oral Presentation [OP-030]
COMMON SENSE-SELF REGULATORY MODEL PADA PASIEN DIABETES MELLITUS TIPE 2 DI RSUD ABDUL AZIS SINGKAWANG, RS DOK II JAYAPURA DAN
RSUD KAB. KEP. MERANTI TAHUN 2018
Bambang Purwoko1), Dyah Aryani Perwitasari2),
Imaniar Noor Faridah2), Woro Supadmi2), Ajeng Diantini3) 1)Pascasarjana Farmasi Universitas Ahmad Dahlan Yogyakarta
2)Fakultas Farmasi Universitas Ahmad Dahlan Yogyakarta 3)Fakultas Farmasi Universitas Padjajaran Bandung
ABSTRAK
Latar Belakang: Diabetes mellitus adalah penyakit metabolisme yang ditandai dengan peningkatan kadar
gula darah yang disebabkan kegagalan sekresi insulin. Tujuan: untuk mengetahui hubungan antara persepsi
dan distress pasien DMT2 terhadap kepatuhan, mengetahui hubungan antara kepatuhan terhadap luaran
terapi dan kualitas hidup. Metode: observasional dengan pendekatan cross-sectional, pengambilan data
dilakukan secara prospektif pada pasien DMT2 yang memenuhi Kriteria inklusi dan menjalani pengobatan
rawat jalan. Penelitian ini menggunakan kuesioner DDS, B-IPQ, MMAS, dan EQ-5D. Analisis data
menggunakan SEM yang berbasis varians yaitu PLS 3.0. Hasil: Terdapat 217 pasien DMT2 yang memenuhi
kriteria inklusi pasien perempuan (59%) sedangkan laki-laki (41%). Nilai rerata kualitas hidup adalah nilai
indek (0,78±0,26) dan VAS (71,46±14,45), rerata nilai tertinggi persepsi adalah domain kontrol pengobatan
(8.11±1,89). Nilai rerata kepatuhan (5,37±2,08).Nilai rerata tertinggi pada DDS-17 adalah domain manajemen
terapi (2,55±1,08). Persepsi dan tingkat stress tidak terbukti berpengaruh signifikan terhadap kepatuhan
(p>0.05). Kepatuhan berpengaruh signifikan terhadap luaran terapi (p<0.05). Kepatuhan tidak berpengaruh
terhadap kualitas hidup ( p> 0.05). Tingkat stress dan persepsi tidak berkorelasi dengan luaran terapi melalui
kepatuhan (p>0,05, p>0,05) dan dengan kualitas hidup melalui kepatuhan (p>0,05,
p>0,05). Kesimpulan: Konsep CSSRM pada penelitian ini adalah kepatuhan berpengaruh signifikan pada
hasil luaran terapi tetapi tidak terbukti berpengaruh signifikan pada kualitas hidup.
Kata kunci: DMT2, CSSRM, SEM
29
Oral Presentation [OP-031]
ATYPICAL ANTIPSYCHOTIC INDUCED WEIGHT GAIN IN SCHIZOPHRENIC
PATIENTS
1)Tetie Herlina, 2) Dyah Aryani Perwitasari, 2) Haafizah Dania, 3) Santi Yuliani, 4) Melisa I. Barliana
1)Pascasarjana of Pharmacy, Ahmad Dahlan University, Yogyakarta
2)Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta 3)Mental Hospital Prof. Dr. Soerojo, Magelang
4)Faculty of Pharmacy, Padjajaran University, Sumedang
Coresponding author: [email protected]
ABSTRACT
Atypical antipsychotic are widely prescribed and has potential to cause weight gain, which may result in
development of metabolic syndrome. There is an urgent clinical need to monitor the use of atypical
antipsychotic for metabolic disturbance. This study aims to find out the number of patients who have weight
gain after using atypical antipsychotic. Through retrospective design, data were collected on 80 adult
psychiatric inpatient (20 women and 60 men) with diagnoses schizophrenia. The patients hospitalized January,
2019 through March, 2019, within medium term (over 4 weeks) of initiation atypical antipsychotic. The results
of the patients (20 women and 60 men) receiving atypical antipsychotic with the mean age was 35.6 years,
70% women and 56.7% men had a weight gain of 1–5 kg over a period of 4 weeks. The mean weight observed
among our subjects increased from 57.55±10.743 kg to 59.83±12.205 kg after initiating treatment. The most
widely used atypical antipsychotic agent is combination risperidone-clozapine with a percentage equal to
83.75%. We conclude that medium-term treatment with atypical antipsycotic promoted a substantial weight
gain. Pharmacist and clinicians are encouraged to monitor weight and blood levels parameters in patients
receiving atypical antipsycotic.
Keywords: Atypical Antipsycotic, Weight Gain, Schizophrenia
30
Oral Presentation [OP-032]
POLA PERESEPAN OBAT GASTRITIS PADA PENGOBATAN MIALGIA DI SALAH SATU PUSKESMAS KOTA BANDUNG
Sri Setiatjahjati, Mashum Widiastuti
Email : [email protected]
ABSTRAK
Salah satu penyebab gastritis adalah penggunaaan obat Anti Inflamasi Non Steroid (AINS). Penelitian yang
dilakukan di salah satu Puskesmas Kota Bandung dengan mengambil data penggunaan obat AINS dan obat
gastritis pada pasien myalgia. Hasil evaluasi didapat AINS yang paling banyak digunakan adalah Asam
Mefenamat (628,62 DDD/1000 pasien) dengan jumlah kasus sebanyak 148 penggunaan atau sebesar 53,62
%. Golongan obat gastritis yang digunakan untuk mengurangi efek samping obat AINS tersebut sebanyak 3
jenis dan obat yang masuk dalam segmen 90% adalah ketiga jenis obat tersebut yaitu Antasida, Ranitidin dan
Omeprazol. Analisis data secara kuantitatif menggunakan metode ATC/DDD menunjukkan bahwa obat
gastritis yang paling banyak digunakan adalah Ranitidin (619,57 DDD/1000 pasien) dengan penggunaan
sebesar 171 kasus atau sebesar 61,96 %. Sedangkan pola kombinasi pengobatan yang paling banyak
digunakan adalah kombinasi Asam Mefenamat dan Ranitidin sebasar 81 penggunaan atau sebesar 29,36 %.
Kata kunci : Gastritis, Anti Inflamasi Non Steroid, Myalgia
31
Oral Presentation [OP-033]
THE USE OF ANTI EPILEPTIC DRUGS IN CHILDREN PATIENTS
IN PKU MUHAMMADIYAH BANTUL GENERAL HOSPITAL
Nur Indriyastuti1), Dyah Aryani Perwitasari2), Nurkholid Umam Kurniawan3) 1)Pascasarjana of Pharmacy, Ahmad Dahlan University, Yogyakarta
2)Faculty of Pharmacy Ahmad Dahlan University, Yogyakarta 3)PKU Muhammadiyah Bantul General Hospital, Yogyakarta Coresponding author : [email protected]
ABSTRACT
Epilepsy is one of the most common causes of child morbidity, which causes various problems including
learning difficulties, growth and development disorders, and determining the quality of life of children. Valproic
acid is one of the most commonly prescribed antiepileptic drugs because it has proven effective in the
treatment of all types of epilepsy. The purpose of this study was to understand the use of antiepileptic drugs
in pediatric outpatients at PKU Muhammadiyah Bantul General Hospital by comparing the recurrence time
after the first treatment, the frequency of recurrence during the treatment period, the period between
recurrences and the dose adjustment that recorded in the patient's medical record.This study was carried out
with cohort design. Data retrieval was done retrospectively in May-June 2019. The population in this study
were all pediatric epilepsy patients who at the time of treatment had been treated for two consecutive years or
more who met the inclusion criteria. We collected 92 patients consisting of 52 (26 man, 26 woman) patients
with valproic acid therapy, 37 (17 man, 20 woman) patients with phenytoin therapy and 3 woman patients with
phenobarbital therapy Median duration therapy of valproic acid is 45.15 months, phenytoin 41.73 months,
phenobarbital 54.67 months. We will complete the data on age, treatment history, drug dose, history of
recurrence during the treatment period and period of recurrence on age and conduct data analysis.Univariate
analysis will be use to determine patient characteristics and characteristics of the drug used. T-test will be use
to determine the relationship of the drugs effectiveness.
Keywords : Valproic Acid, Anti Epileptic Drugs, Effectiveness
32
Oral Presentation [OP-035]
ANTITUMOR ACTIVITY OF N-HEXANE FRACTION OF CEMPAKA KUNING BARK (Michelia champaca Linn.) ON BREAST CANCER CELLS MCF7 AND IT’S ACUTE
TOXICITY TEST
Ade Zuhrotun1*, Yuni Elsa Hadisaputri1, Imam Adi Wicaksono2, Rizki Muhammad Zafrial3, Wahyu Ashri Aditya3
1Biological Pharmacy Department, 2Pharmacology and Clinical Pharmacy Department, 3Undergraduate Pharmacy Study Program
Faculty of Pharmacy Universitas Padjadjaran Jl. Raya Bandung Sumedang KM 21, Jatinangor 45363
*e-mail: [email protected]
ABSTRACT
Extract and n-hexane fractions of cempaka kuning bark (Michelia Champaca Linn.) have been proven by in
vitro study are active as topoisomerase inhibitors, one of the mechanisms of anticancer drugs. This study was
conducted in vivo test the antitumor activity of n-hexane fraction and it’s toxicity test. Female mice, Balb/c
strains, that have tumors after being induced s.c. with MCF7 breast cancer cells, then given a p.o of the
fraction at doses 3.75; 7.5 and 15 mg / Kg BB. The observations for 25 days showed that 8 of 9 mice tested
had recovered from the tumor with a percent reduction in tumor volume was 99.3 ± 1.95%. In the acute toxicity
test, male and female Swiss Webster mice were given n-hexane fraction at doses 2.8; 28; 280; 2800 and
28000 mg / kgBW. In general, it does not show symptoms of toxicity. This symptom is only seen in mice that
are given the highest dose, only at the effect of retablismen and piloerection. So that the value of LD50> 28000
mg / kgBB on mice or equal to 19600 mg / kgBB on rats so that the n-hexane fraction is classified as relatively
harmless. It can be concluded that the n-hexane fraction of cempaka kuning bark has antitumor activity, proven
to be safe in short-term use so that it is prospective to be further developed as a natural anti-breast cancer
Keywords: Cempaka Kuning, n-Hexane Fraction, Acute Toxicity, MCF7, Anti-Breast Cancer
33
Oral Presentation [OP-036]
COMMON SENSE SELF REGULATORY MODEL (CSSRM) AND MEDICATION
BELIEFS IN DIABETIC PATIENTS USING ANTIHYPERTENSION MEDICATION AND / OR ANTIHYPERLIPIDEMIA IN YOGYAKARTA REGIONAL GENERAL HOSPITAL
Andrey Wahyudi¹, Dyah Aryani Perwitasari², Rizky Abdullah³, Sofa Dewi Alfian
Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta ¹ Faculty of Pharmacy, Padjadjaran University, Bandung²
ABSTRACT
The aim of this study was to investigate the association between medication beliefs regarding adherence, the
relationship between medication beliefs, diabetes distress, perceptions and characteristics of quality of life and
outcome of therapy in T2DM patients in Yogyakarta City Hospital. The design of this study is cross sectional.
The questionnaire used in this study was Beliefs about Medications Questionnaire (BMQ) specific, Medication
Adherence Report Scale (MARS), European Quality of Life-5 Dimensions (EQ-5D), Diabetes Distress Scale
(DDS), and Brief Perception Questionaire (B-IPQ). The therapeutic output measured is GDP, G2PP, HDL,
LDL, blood pressure, triglycerides, and total cholesterol. The tool used is Partial Least Square (PLS), which is
SEM based on variance, with SmartPLS 3.0 software as an analysis. The number of patients in this study was
50 people consisting of male patients by 42.0% and 29.0% women, with an average age of 46-79 years
(63.84%). These findings indicate that there are differences of opinion about treatment beliefs in the meeting
(p<0.10, p<0.10), consideration of treatment confidence in treatment outcomes in T2DM patients with
hypertension (p<0.10), and not in accordance with patients DMT2 with hyperlipidemia (p>0.10), challenge
against outcome therapy (p <0.10, p <0.10), motivation for perception of therapeutic outcome (p<0.10, p<0.10),
need for medication confidence in bonded therapy outcomes (p<0.10, p <0.10), and there were differences in
consultation with outpatient therapy (p<0.10) in T2DM patients with hypertension, none of whom were asked
for DMT2 patients with hyperlipidemia (p>0.10), There is no contradiction between quality, treatment beliefs
about quality of life, perception of quality of life, therapy for quality of life, outcome of therapy, and combination
(p>0.10). The concept of CSSRM in this study is treatment beliefs and positive perceptions of patients on
treatment to increase help, with high coverage it is agreed upon the results of well-controlled therapeutic
outcomes. While distress and quality of life have less implications in this study.
Keywords: DMT2, Medication Beliefs, CSSRM
34
Oral Presentation [OP-038]
COMMON SENSE-SELF REGULATION MODEL IN DIABETES MELLITUS PATIENTS USING ANTIHYPERTENSION AND/OR ANTIHYPERLIPIDEMIA MEDICINE IN PKU
MUHAMMADIYAH GAMPING YOGYAKARTA HOSPITAL
Nanny Iriany Marsidin1, Dyah Aryani Perwitasari1, Rizky Abdullah2, Sofa Dewi2
1)Fakultas Farmasi Universitas Ahmad Dahlan Yogyakarta 2) Fakultas Farmasi Universitas Padjajaran Bandung
e-mail address of corresponding author : [email protected]
ABSTRACT
Diabetes mellitus is a chronic metabolic disorder, characterized by high blood glucose levels. The aim of this
research is to determine the relationship between medication beliefs, perceptions, diabetes distress with
adherence and determine the relationship between medication beliefs, diabetes distress, perceptions,
characteristics with quality of life and outcome of therapy in T2DM patients in PKU Muhammadiyah Gamping
Hospital, Yogyakarta. The cross sectional design was used as method, data was collected with the inclusion
criteria being over 18 years old, diagnosis of T2DM for one year using antihypertensive and/or
antihyperlipidemia drugs within three months and can read the questionnaire. Beliefs about Questionnaire
(BMQ-specific), Medication Adherence Report Scale (MARS), European Quality of Life-5 Dimension (EQ-5D),
Diabetes Distress (DDS), and Brief-Illness Perception Questionnaire (B-IPQ) used as Questionnaire. Fasting
Blood Glucose (GDP), blood pressure and total cholesterol measured as therapeutic output. Data analyzed
with SEM ( Structural Equation Modelling) based in variance, namely Partial least Square (PLS) version of
SmartPLS 3.0. there were 64 participant as respondents, consist of female (64,1%) and male (35,9%) with 33
to 79 years old. Medication beliefs had no correlation with Adherence (1,373; 0,695), with Quality of life (1.069;
0,411) and treatment outcome through adherence (0,315; 0,318). Correlation between Perceptions to quality
of life 2,138(Tstat>1,96) with quality of life in T2DM patient with hypertension directly or through adherence, but
didn’t have correlation with T2DM patients with hyperlipidemia 0,581(Tstat <1,96). No correlation between
Perceptions to quality of life 0,814;0,563(Tstat<1,96) with treatment outcomes through Adherence 0,094;
0,435(Tstat<1,96). Diabetic Distress had correlation with adherence T2DM Patients with hyperlipidemia
3,255(Tstat>1,96), but no correlation with T2DM patient with hypertension 0,825(Tstat<1,96), No correlation
between Diabetic distress with Qualitry of life 0,496; 0,911(Tstat<1,96), and treatment outcome through
Adherence 0,214; 0,707(Tstat<1,96), No correlation Adherence to Quality of life 1,280; 0,836(Tstat<1,96), and
treatment outcome 0,335; 0,630(Tstat<1,96). This reseacrh found the positive perception about CSSRM and
diabetic distress can increased adherence. Adherence can improve the quality of life patients and no effect
between medications beliefs and treatment outcomes.
Keywords : DMT2, CSSRM, Medication Beliefs
35
Oral Presentation [OP-039]
PENGARUH BRIEF COUNSELING 5A APOTEKER TERHADAP PENGETAHUAN,
PERSEPSI, DAN KEPATUHAN IBU HAMIL YANG MENGKONSUMSI FERROUS
FUMARATE DI PUSKESMAS BANGUNTAPAN III BANTUL
Endang Darmawan1, Sri Suprapti1, Akrom1,Sulistiari Retnowati2
1Program Pascasarjana Farmasi Klinis, Fakultas Farmasi, Universitas Ahmad Dahlan Yogyakarta 2RS PKU Muhammadiyah Yogyakarta
Korespondensi: [email protected]
ABSTRAK
Anemia pada kehamilan merupakan masalah umum di negara berkembang yang mengakibatkan peningkatan
mortalitas ibu dan janin. Tujuan penelitian ini adalah mengetahui pengaruh brief counselling 5A terhadap
pengetahuan, persepsi dan kepatuhan, serta monitoring efek samping (ESO) pada ibu hamil yang
mengkonsumsi ferrous fumarate di Puskesmas Banguntapan III Bantul. Penelitian ini merupakan quasi
eksperimental pre and post test without control group design, secara prospektif pada Desember 2018-April
2019. Subjek penelitian hingga akhir sebanyak 55 ibu hamil dan dilkukan pengukuran terhadap tingkat
pengetahuan, persepsi, kepatuhan sebelum (hari ke-1) dan setelah (hari ke-31) mendapaatkan brief
counselling 5A yang dimodifikasi oleh peneliti, serta dilakukan monitoring ESO ferrous fumarate. Hasil
penelitian menunjukkan bahwa pemberian brief counselling 5A dapat meningkatkan skor rerata pre-test dan
post-test tingkat pengetahuan, persepsi dan kepatuhan secara signifikan (p= 0,000) dengan rerata skor pre
test pengetahuan 63,65±2,25 dan nilai post test 89,27±1,24. Rerata skor persepsi pre test 27,71±0,49 dan
post test 33,55±0,52.Rerata skor tingkat kepatuhan pre test 56,91±2,74 dan post test 86,91±1,62. ESO
terbanyak dari penggunaan ferrous fumarate.adalah feses hitam (16,4%). Berdasarkan hasil penelitian ini
dapat disimpulkan bahwa, pemberian brief counselling 5A dapat meningkatkan pengetahuan, persepsi, dan
kepatuhan ibu hamil yang mengkonsumsi ferrous fumarate di Puskesmas Banguntapan III Bantul dengan
sebagian besar subjek penelitian tidak mengalami ESO (52,7%).
Kata kunci: Anemia, Brief counselling, Ferrous fumarate, Pengetahuan
36
Oral Presentation [OP-040]
ANEMIA PREVALENCE AMONG WOMEN OF REPRODUCTIVE AGE IN TELUK
BINTUNI REGENCY, WEST PAPUA, INDONESIA
Yasinta Rakanita1) 5)* , Rizky Abdulah1), Aulia A Suwantika1) , Rano K Sinuraya 1) , Mas Rizky ASS2) , Eka Suradji3) , Kristina4)
1) Pharmacolog and Clinical Pharmacy Department, Pharmaceutical Faculty, Padjajaran University,
Bandung, West Java, Indonesia 2) Department of Biochemistry and Molecular Biology, Medicine Faculty, Padjajaran University,
Bandung, West Java, Indonesia 3) Teluk Bintuni Hospital, Regency Health Office of Teluk Bintuni, West Papua, Indonesia
4) Public Health Department, Regency Health Office of Teluk Bintuni, West Papua, Indonesia 5) Pharmacy Degree Program, Sekolah Tinggi Ilmu Farmasi Pelita Mas, Palu, Indonesia
*Correspondence e-mail : [email protected]
ABSTRACT
Many of developing countries burdened with anemic conditions. Based on World Bank Data 2019, the trend
of anemia prevalence in pregnant women in Indonesia since 2009-2016 has increased. After succeeding in
dropped in 2008 at 34.6%. Of course, there is some factor associated with that. According to this condition,
This study had choosen the location which have the heaviest burden of anemia. It is in West Papua. The
purpose of study is to analyze anemia prevalence in moderate-severe, and associated factors anemia among
women reproductive of age in West Papua Province, Teluk Bintuni Regency. The design method was cross
sectional. Sampling collected from 6 districts randomly selected by inclussion criteria. The result is as many
as 875 reproductive age women are examined by modified quesionners and for hemoglobin levels by
HemoCue™ 201 analyzer. Found as many as 467; 222 women of reproductive age suffer from mild, and
moderate to severe anemia, respectively. Then obtained an anemia prevalence of 53.3% and almost half of
them had moderate to severe anemia. Trend indicates that the burden of infection occurs in the community as
one of the main causes of anemia. The program is to control the infection burden.
Keywords: Prevalence, Anemia, Women of Reproductive Age
37
Oral Presentation [OP-041]
ANTI HAIR LICE SHAMPOO OF SEED AND LEAF INFUSA FROM PUCUNG (Pangium edule Reinw)
Ine Suharyani1), Laila Fitriani Nur Aisyah1), Oi Surya Diharja1)
1)STIKES Muhammadiyah Kuningan
e-mail address of corresponding author : [email protected]
ABSTRACT
Hairlice is a parasit that very harm, especially in humans. Pucung is one of the most plant at Indonesia which
kill the lice. The aim of this reasearch is to formulategel shampo contining leaf and seed pucung infusan as
anti hair lice and to know the physical stability during storage and hedonic test. The reasearch doneintofour
formulation with concentration variation ofleafand seed pucung infusanwere 5%, 10%, 15%, 20% with Na CMC
3% as a basis. Effected test showed that time for hair lice dead 100% used shampoo concentration 5% is 1
minute 37 second, concentration 10% is 1 minute 17 second, concentration 15% is 1 minutes 1 second and
concentration 20% is 34 second. Iritation test to rabbit showed that iritation indeks is 0,00. That value showed
that the gel shampoo anti hair lice of seed pucung infusan not effected to skin and eye.
Keywords: Hair Lice, Pucung, Gel Shampoo, Hedonic Test
38
Oral Presentation [OP-042]
MANAGEMENT OF LOOK ALIKE AND SOUND ALIKE (LASA) DRUGS AS HIGH ALERT MEDICINE TO REDUCE DISPENSING ERROR
Nur Miftahurrohmah, Ayu Deyana Cynthia
Faculty of Pharmacy, Universitas Pancasila, Srengseng Sawah, Jagakarsa, Jakarta Selatan, DKI
Jakarta, Indonesia, 12640
e-mail address of corresponding author : [email protected]
ABSTRACT
Some types of drugs have similar names, shapes, and sounds (look alike sound alike / LASA). In Indonesia,
this drugs are categorized as NORUM (Nama Obat, Rupa, Ucapan Mirip). LASA drugs are included in high
alert drugs, because of the high risk of causing serious errors and unwanted drug reactions. To minimize errors
in taking LASA drugs in the dispensing process, clear management is needed regarding the storage,
placement, labelling, and inspection. This study aims to find out examples of drugs classified as LASA and
their management in hospitals. The method used is by direct observation at a hospital pharmacy installation
in Jakarta, Indonesia. The results showed that drugs including the LASA category included drugs with the
same name but different doses, same names with different dosage forms, similar dosage forms with similar
packaging. The handling of LASA drugs applied at the hospital includes the provision of LASA stickers, storage
which is interspersed with other drugs, application of tall man lettering system, and rechecking step. The
application of good LASA drug management and the compliance of pharmacy staff to do the rules stated in
management of LASA drugs are the important keys in the accuracy of therapy and patient safety.
Keywords: Look Alike, Sound Alike, LASA, NORUM, High Alert.
39
Oral Presentation [OP-044]
THE EFFECTS OF COUNSELING AND DRUG INFORMATION SERVICES BY PHARMACIST TO MEDICATION ADHERENCE AND THERAPY OUTCOMES ON
SCIATICA PATIENTS AT DR. SUTOYO HOSPITAL, JAKARTA
Farida Ariyani1), Prih Sarnianto2), Nurita Andayani 2)
1)Master Program in Pharmaceutical Sciences, Pancasila University 2)Faculty of Pharmacy Universitas Pancasila
Corresponding author: Prih Sarnianto, [email protected]
ABSTRACT
People suffered from sciatica, pain along the pelvic nerve, require therapy up to 4 months or longer to achieve
controlled-pain. The main treatment for sciatica is physiotherapy; medication is only secondary therapy. This
quasi-experimental study is intended to investigate the effects of counseling and drug information services
(DIS) by pharmacist on medication adherence and duration of therapy. In the 16 week-study, 128 ambulatory
sciatica patients newly diagnosed at Dr. Suyoto Hospital, Jakarta, were divided randomly into two groups, each
of 64 patients. The intervention group was given counseling and DIS by pharmacist (3 times with interval of 4
weeks) as well as brochures. Primary data on medication adherence were obtained prospectively through
interviews using MMAS-8 questionnaire, before the intervention and 4 weeks after the last counseling and
DIS. Secondary data, including pain scores, were obtained from medical records and the patient administrative
data. The results showed that sociodemographic characteristics as well as distribution of initial adherence and
initial pain levels of the two groups were not significantly different (Mann-Whitney test, p>0.05). After the last
intervention, the proportion of patients with high-adherence in the intervention group (12.5%) was higher than
in the control group (6.3%), as was the proportion of patient achieved controlled-pain (84.4% versus 76.6%).
The results of survival analysis showed that the duration of treatment by which 50% of patients achieved
controlled-pain (median survival) was significantly shorter in the intervention group (85 days) than in the control
group (92 days), while the probability of achieving controlled-pain (hazard ratio) in the intervention group was
1.7 times of that in the control group. In conclusion, counseling and DIS by pharmacist can improve the
effectiveness of sciatica treatment, although the main therapy for the nerve pain is not medication.
Keywords: Sciatica, Counseling By Pharmacist, DIS, Median Survival
40
Oral Presentation [OP-045]
COMPLIANCE OF DRUG USE IN-PATIENT HYPERTENSION PATIENTS, ONE OF THE RSUD IN YOGYAKARTA PRIVATE REGIONAL REGION
Muhammad Muhlis 1), Alissa Jihan Prameswari 1)
1). Fakultas Farmasi UAD Yogyakarta
corresponding author : [email protected]
ABSTRACT
The study aimed to determine the description of compliance with drug use and the relationship between
distance of health services, duration of illness, and other illnesses to compliance with drug use in outpatient
hypertension patients in the period January-February 2019 in One Hospital in the Special Region of
Yogyakarta.
This study was an observational study with a cross sectional design. Sampling using a questionnaire using
accidental sampling technique as many as 96 patients. Compliance was analyzed using Univariate and
Bivariate analysis with the chi square method.
The results showed that there were 78 patients in the obedient category (81.3%). Chi square test obtained
distance of health service p = 0.462; OR = 1,690 (0,6024,741) and the presence of other diseases p = 0,818;
OR = 1,294 (0,464-3,613) there is no significant relationship with compliance; long suffering from hypertension
there was a significant relationship with compliance p = 0.005; OR = 6.471 (1,732-24,171).
Conclusion, hypertension patients are categorized as obedient 81.3% and there is no significant relationship
between distance of health services and other diseases with adherence. But there is a significant relationship
between the duration of suffering from hypertension and compliance.
Keywords: hypertension, compliance, outpatient
41
Oral Presentation [OP-046]
ADVERSE DRUG EVENT (ADE) OF COMBINATION RISPERIDONE AND CLOZAPIN THERAPY IN SCHIZOPHRENIA PATIENT AT MENTAL HOSPITAL MAGELANG
Rizki G Al Farizi1, Dyah A Perwitasari2, Melisa I Barliana3, Hafizah Dania4, Santi Yuliani5 1) Postgraduate Clinical Pharmacy Program, Faculty of Pharmacy, Ahmad Dahlan University,
Yogyakarta 2)Clinical Pharmacy Department, Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta
3)Biology Pharmacy Department, Faculty of Pharmacy, Padjajaran University, Bandung 4)Clinical Pharmacy Department, Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta
5)Psychiatrist, Mental Hospital Prof Dr. Soerodjo, Magelang, Centre Java
Author Correspondence: [email protected]
ABSTRACT
Combination between risperidone and clozapin used by healthcare provider especially for handle
schizophrenia patients that unresponsiveness to antipsychotic monothraphy. Adverse Drug Event (ADE) of
risperidone and clozapin combination theraphy such as increase of body mass index, systolic blood pressure
and diastolic blood pressure can occur during along the duration of theraphy. This research was conducted to
identify the relationship between duration of combination risperidon and clozapin theraphy to increase of body
mass index, systolic blood pressure and diastolic blood pressure. The method used in this research was cohort
study design from February until May 2019 at Mental Hospital Magelang with 59 schizophrenia inpatient based
on inclution criteria that gruped into 2 category duration of theraphy less than 8 weeks for 48 patients and more
than equal 8 weeks for 11 patients. Body mass index, systolic blood pressure and diastolic blood pressure
was measured when first day hospitalized and outpatient after hopitalized based on cut off-point NCEP ATP
III with modification of body mass index Southeast Asian population and structure quistionnaire for measure
calories intake and physical activity that obtained from patient medical record. There were indicate significance
relation between increase of body mass index before and after hospitalized which grup more than equal 8
weeks 22.4 kg/m2 vs 25.2 kg/m2 (p=0.01, 95%CI =0.9–1.838), but there ware not significant defference systolic
blood pressere 117 mmHg vs 118 mmHg (p=0.486, 95%CI =-3,53 – 5.095), and diastolic blood pressure 76
mmHg vs 73 mmHg (p=0.163, 95%CI =-3.3 – 3.113) among two grups. Counfounding factors such as age (p
=0.501), gender (p =0.501), smoking status (p=1.000), dosage (p=0.322), co-medocation (p=0.163), calories
intake (p=0.292), and physical activity (p=0.187) didn’t show significant difference. In conclusion, duration of
combination risperidone and clozapin theraphy were statistically affect on increase of body mass index, but no
clinically significant
Keyword: Metabolic Syndrome, Risperidone and Clozapin Combination Theraphy, Schizophrenia
42
Oral Presentation [OP-047]
VALIDATION OF PORCINE GELATIN USING SPECIFIC TARGETING ON D-LOOP BY REAL-TIME POLYMERASE CHAIN REACTION
Nina Salamah1,4, Yuny Erwanto2,3, Sudibyo Martono1 and Abdul Rohman1,3
1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
2Division of Animal Products Technology, Faculty of Animal Science, Universitas Gadjah Mada, Jl. Fauna No. 3, Bulaksumur, Yogyakarta 55281, Indonesia.
3Research Centre of Halal Products, Universitas Gadjah Mada, Jl. Kaliurang Km 4, Sekip, Yogyakarta 55281, Indonesia.
4Department of Analytical Chemistry, Faculty of Pharmacy, University of Ahmad Dahlan, Jl.Prof Soepomo, Janturan Yogyakarta, Indonesia
e-mail address of Corresponding author: [email protected]
ABSTRACT
Background: Specific primer design is an interesting research topic for the analysis of identification using real-
time PCR selectively, specifically, and effectively. This research is very important in relation to the method of
analyzing porcine DNA identification using real-time PCR with specific primers in various products to issue
halal status. Method: The porcine DNA primer is designed using the NCBI and Primer BLAST software.
Validation parameters performed are specifications, repeatability test and linearity by real-time PCR. Analysis
for the primer specificity performed on fresh tissue (cows, chickens, dog, goats, rabbit and negative control).
repeatability test is done with 6 replications and the coefficient of variation (CV) value is calculated. Linearity
is carried out at 7 different DNA concentrations (50000, 5000, 500, 50, 10, 0,5 and 0,1 pg) to get Efficiency
value. The method is expected to specific, sensitive and reliable for the analysis of porcine DNA. Result: The
results show that the real-time PCR with primer D-LOOP specifically able to identify the presence of porcine
DNA at optimum annealing temperature 50,2 C. The coefficient of variation (CV) on repeatability analysis was
0,57 % and the efficiency value on linearity analysis was 100%. Conclusions: Real-time PCR using D-LOOP
porcine primer (forward: ACTTCATGGAACTCATGATCCG; reverse ATGTACGTTATGTCCCGTAACC) can
used for identification of porcine DNA.
Keywords: Primer D-LOOP, Porcine DNA, Specific, Real-Time PCR
43
Oral Presentation [OP-048]
SELECTIVITY ANALYSIS OF APIGENIN DERIVATIVE COMPOUNDS ACTING AS
ANTIOXIDANT USING MOLECULAR DOCKING METHOD
Esti Mulatsari1), Titiek Martati1), Esti Mumpuni1), Frans Wijaya1)
1)Faculty of Pharmacy, Pancasila University
e-mail address of corresponding author : [email protected]
ABSTRACT
Free radicals are a group of chemicals compound that can trigger occurrence of degenerative diseases dan
required antioxidants to counter it. The examples of antioxidants compounds are vitamin C, vitamin E, and
flavonoids such apigenin with IC50 values of 463,4 μg/ml. The purpose of this study was to find alternative
antioxidants that may have better activity than the existing antioxidants using the molecular docking method
using target receptors with 1QQW, 1V4S, 2BEL, 2C9V, 4K7O, and 5M2F PDB codes that met the requirements
of RMSD values less than 2 Ǻ and using vitamin C, vitamin E, quersetin and apigenin as comparable
compounds that have been tested to have antioxidant activity. Of the 30 apigenins derived compounds used
with an IC50 value below 100 μg/mL, one of the best compounds of each receptor with the lowest ChemPLP
values was 3,3’-diisopropoksi apigenin(1QQW); 3’-amina apigenin(1V4S); 3’,6-diamin apigenin(1XAN); 3’,6-
diamin apigenin(2BEL); 3’-amina apigenin(2C9V); 3’-amina apigenin(4K7O), dan 3’6-dipropil apigenin(5M2F).
From the results obtained there are apigenin derived compounds that are selective against receptors used.
Keywords: Apigenin, Derivative Compound, Antioxidant, Molecular Docking
44
Oral Presentation [OP-049]
THE AVAILABILITY OF ANTI-HYPERTENSION DRUG IN ERA OF JKN: A STUDY IN THE PRIMARY HEALTH CARE CENTRE JAKARTA-INDONESIA
Mita Restinia1*, Dian Ratih Laksmitawati1, Yusi Anggriani1, Yudha Sekowati2
(1) Faculty of Pharmacy, University of Pancasila, Jl. Srengseng Sawah,
Jagakarsa, Jakarta 2)Academy Pharmacy IKIFA, Jakarta
e-mail address of corresponding author : [email protected]
ABSTRACT
The study was carried out to observe the impact of national health insurance (NHI) program called Jaminan
Kesehatan Nasional (JKN) to availability, usage, stock of anti-hypertension drug in one of primary health care
centre in Jakarta. A longitudinal time series design was performed retrospectively to analyse the availability,
usage, and stock of amlodipine 5 mg and 10 mg tablet, hydrochlorothiazide 25 mg tablet, captopril 12.5 mg
and 25 mg tablet, nifedipine 10 mg tablet with data period from January 2013 (1 year before NHI) to December
2015 (2 year after NHI). The Wilcoxon test was conducted to compare these parameters before and after NHI.
The result of study showed that the availability and usage of amlodipine 5 mg tablet increased significantly
(P<0.05) after NHI. Amlodipine 10 mg tablet was not available in 2013 and 2014. The availability and usage
captopril 12,5 mg tab increased (P<0,05) as well. However, the availability of captopril 25 mg tablet was not
different. Furthermore, the availability and usage hydrochlorothiazide 25 mg tablet decreased significantly
(P<0,05) after NHI. The stock of all anti-hypertension drugs was constant. In conclusion, this study highlights
the implementation of NHI influenced the availability and usage of hypertension drugs.
Keywords: NHI, Drug Availability, Drug Usage, Drug Stock, Anti-Hypertension Drug.
45
Oral Presentation [PP-050]
DETERMINATION OF MAJOR COMPONENT OF ETHYL ACETATE FRACTION OF KATUK LEAVES (Sauropus andogynus L.)
Resmi Mustarichie1)*, Yoppi Iskandar2), Tiara Salsabila1)
1) Pharmaceutical Analysis and Medicinal Chemistry Dept., Faculty of Pharmacy, Universitas
Padjadjaran, Indonesia 45363 2) Biology Pharmacy Dept., Faculty of Pharmacy, Universitas Padjadjaran, Indonesia 45363
ABSTRACT
Katuk leaves can be used in jamu (Indonesian herbal medicine), which empirically is shown to restore uterus
and abdomen to its normal size post giving birth. Katuk is also often believed to increase lactation. The locals
of Mak Kemas Village, Malaysia, processed Katuk leaves to cure baldness and increase hair growth. The
purpose of this study is to identify the optimum method to obtain one of the chemical compounds in the water
fraction of katuk leave’s ethanol extract and to identify the hypothesized chemical isolates in the water fraction
katuk leave’s ethanol extract. The method that was used consists of several steps, which are collecting and
determining the katuk plants, processing the katuk, phytochemical filtration, extraction using ethanol 96%,
fractionation using the liquid-liquid extraction method with n-Hexane:Ethyl Asetate:Water solvent, active
compound isolation using column chromatography and active compound analysis using the UV-Vis
spectrometry, IR spectrometry and LC-MS. The results shown the isolate in the form of a 715.4 mg of white
powder. The isolate is in the compound with a molecule mass of 406.3131 m/z with C21H39N6O2 compound
prediction.
Keywords: Sauropus androgynus (L.) Merr, Isolation, Identification, C21H39N6O2
46
Poster Presentation [PP-001]
COMPARISON OF HYPOGLICEMIC EFFECT OF EXTRACT RINDS, FLESHS, SEEDS
AND FLOWERS Musa balbisiana Colla IN A MICE
Tita Nofianti1, Ahmad Muhtadi1, Irda Fidrianny2
1) Department of Pharmacology, Faculty of Pharmacy, Padjadjaran University, Bandung, Indonesia
2) Department of Pharmacognosy, School of Pharmacy, Bandung Institute of Technology, Bandung,
Indonesia
ABSTRACT
Banana is a plant that grows a lot in Indonesia and is a fruit that is widely consumed by Indonesian people in
all circles. One type of banana is klutuk. Klutuk bananas have antidiabetic, antihyperlipidemic, antioxidant and
antibacterial activities. So this study aimed to evaluate the antihyperglikemic activity of extract of fruit parts
klutuk banana (rinds, fleshs, seeds and flowers). He research was conducted by performing Oral Glucose
Tolerance Tests (OGTT) by using swiss webster mice devided into 15 groups: negative control, positive
control, standard drug control (glibenclamide 0.65 mg / KgBW), dose I, II, III of extract of klutuk rinds (175 mg
/ KgBW, 350 mg/ KgBW, 700 mg/ KgBW), dose IV,V,VI of ethanol extract of klutuk fleshs (175 mg / KgBW,
350 mg/ KgBW, 700 mg/ KgBW) dose III (300mg / KgBW), dose VII,VIII, IX of extract of klutuk seeds (175 mg
/ KgBW, 350 mg/ KgBW, 700 mg/ KgBW) and dose X, XI,XII of extract of klutuk flowers (175 mg / KgBW, 350
mg/ KgBW, 700 mg/ KgBW). In the OGTT method the mice were given 3g / KgBB glucose monohydrate as a
positive control and blood glucose level was measured every 30 minutes for 120 minutes. Was calculated
OGTT results show that a dose 350 mg/KgBW of rinds and flowers klutuk banana, might decrease blood
glucose levels in 30,60,90 and 120 minute. The conclusion was that extract of rinds and flowers klutuk banana
had antihyperglikemic activity and the most effective dose was 350 mg / KgBW.
Keywords: Musa balbisiana Colla, antihyperglikemic, OGTT
47
Poster Presentation [PP-002]
FORMULATION AND PHYSICAL EVALUATION OF CHLORAMPHENICOL IN SITU GEL WITH BASE POLOXAMER 188 AND HPMC
Insan Sunan Kurniawansyah1, Dolih Gozali1, Muhammad iqbal2
1)Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy Universitas
Padjadjaran 2)Faculty of Pharmacy Universitas Padjadjaran
ABSTRACT
In situ gel ophthalmic preparations are developed for drug delivery forms that mask the shortcomings of conventional forms of ophthalmic drug delivery system. Among the advantages of in situ gel that significantly increase the availability of drugs in the precursor and drug bioavailability by using new drug delivery systems based on the concept of in situ gel formation. This research was conducted in order to obtain the best formulation of in situ gel preparation based on 2 different bases which were Poloxamer 188 and HPMC (hydroxypropyl methylcellulose) along with the evaluation of physical parameters test, such as organoleptic parameter, pH, viscosity and gel capacity for 28 days of time storage. Formulation was performed by selecting 2 formulations from each base with different concentrations. Formulation 1 (F1) and Formulation 2 (F2) each used Poloxamer 188 base with concentrations of 5% and 10% respectively and Formulation 3 (F3) and Formulation 4 (F4) each used the HPMC base with concentrations of 0.45% and 1% respectively. The results of this study showed that Formulation 1 (F1) was the best formulation that have pH 6.45 and viscosity of 5.47 cps.
Keywords: In-situ gel, Poloxamer 188, HPMC
48
Poster Presentation [PP-003]
QUERCETIN OF Etlingera elatior LEAVES AS CYTOTOXIC AGENT AGAINST HeLa
CELL LINES
Kusriani Herni1,2), Subarnas Anas1), Iskandar Yoppi.1) Diantini Ajeng 1)
1) Faculty of Pharmacy, Padjadjaran University, 2)Bandung School of Pharmacy, Indonesia
ABSTRACT
A flavonoid quercetin has been isolated from the leaves of Etlingera elatior. The compound was purified by
using column chromatography with the help of a specific guidance for the cytotoxic compound isolation.
Cytotoxic activity against HeLa cell line using MTT assay method were subjected to extract and fraction to
guide the isolation. The structure of the isolate was determined according to the UV, IR and NMR spectroscopic
data. In this research reported the occurrence of quercetin in Etlingera elatior as cytotoxic compound against
HeLa cell line.
Keyword: Quercetin, Etlingera elatior, cytotoxic, HeLa cell
49
Poster Presentation [PP-004]
THE EFFECTIVNESS OF NEEM OIL (Azadirachta Indica A. Juss) SHAMPOO AS
HEAD LICE (Pediculus humanus capitis) TREATMENT
Norisca Aliza Putriana, Azizul Hakim Bin Sulaiman, Melinda Januarti
Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas
Padjadjaran, Jl Raya Bandung Sumedang km 21 Jatinangor Indonesia 45363
ABSTRACT
Head lice (Pediculus humanus capitis) is a parasite that causes hair problem that often occurs in society. The
aim of this study is to examine the effectiveness of the neem oil shampoo formulation as an antilice. The
method used is an experimental laboratories: formulation of shampo, irritation test treatment, testing the
effectiveness of shampoo againsts head lice (Pediculicidal activity) and testing the effectiveness of shampoo
against nits (Ovicidal activity). The effectiveness test uses an in-vitro method where neem oil shampoo are
tested directly towards headlices and nits that have been isolated from humans, then we observe their
mortality. To see the safety of the shampoo, skin and eye irritation tests were carried out by applying 0.5ml on
the skin and dripping 0.1ml shampoo into rabbit's eyes. The primary irritation index for 5%, 10%, 15%, and
20% neem oil shampoo respectively are 0 ; 0111; 0.222; and 0.222, all shampoos belong to the very mild
irritation response category. For eye irritation tests, only 20% neem oil shampoo gave a mild irritation response
where the degree of corneal opacity ≥ 1 and recovered after 7 days of observation. 5%, 10%, 15%, and 20%
neem oil shampoo are effective as an antilice. The 20% and 15% neem oil shampoo have the same antilice
effectiveness activity as the 1% permethrin lotion. However, The 15% neem oil shampoo was chosen as the
best shampoo because of its high antilice effect and light irritation response.
Keywords: Head lice, neem oil (Azadirachta indica A. Juss), shampoo
50
Poster Presentation [PP-005]
ANALGESIC AND ANTIINFLAMMATORY ACTIVITY OF METHANOL EXTRACT AND ITS FRACTIONS FROM THE ROOT OF POLYPODIUM FEEI METT
Deden Winda Suwandi 1,2*, Tina Rostinawati1, Muchtaridi1, and Anas Subarnas1
1)Department of Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia 2)Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Garut, Indonesia
ABSTRACT
Polypodium feei roots are commonly used by the community as traditional medicine for rheumatic diseases.
In this research, extract of methanol, fractions of n-hexane, ethyl acetate and water from polypodium feei roots
has been tested using acetic acid-induced writhing, hot plate, and carrageenan-induced paw edema methods,
respectively. The effective dose of extract of methanol is 100 mg/kgbw significantly decreased writhing
responses of mices induced by 0.7 % acetic acid along the 60 min test with percent protection of 49.22 and
percent effectiveness of 98.53. The effective doses fractions of n-hexane, ethyl acetate and water are 200,
100, and 50 mg/kgbw with the percent protection are 69.19; 82,71 and 61,67 % and percent effectiveness are
148.22 ; 63.54 and 90.88%. The effective doses of strong analgesic activity of extract of methanol and fraction
of water are 100 mg/kgbw and fractions of n-hexane and ethyl acetate are 50 mg/kgbw significantly increased
of heat response times of mices with the percent increased are 154.09; 99,57; 95,86 and 143,74. And then,
the effective doses as antiinflammatory of extract of methanol and fraction of n-hexane are 100 and 200
mg/kgbw, and fractions ethyl acetate and water are 50 mg/kgbb with edema inhibition values are 59%, 62%,
57% and 64% caused significant inhibition of the rats plantar edema induced by 1 % of carrageenan.
Keywords: Polypodium feei, analgesic and anti-inflammatory, effective dose.
51
Poster Presentation [PP-006]
PROCESSING OF CATFISH (Clarias garepinus) BONE WASTE AS A POTENTIAL
SOURCE OF GELATIN FOR THE MANUFACTURE OF HARD CAPSULE SHELL
Devi Ratnasari1), Triani Sumiati2), Siti Rosidah Hanapiah2)
1) Faculty of Health Science, University of Singaperbangsa Karawang, Karawang, Indonesia 2) Sekolah Tinggi Teknologi Industri dan Farmasi Bogor, Bogor, Indonesia
ABSTRACT
Capsule is form of drug preparations are enclosed in a hard or soft shell. Capsule shell made from gelatin with
or without additives. Gelatin is a substance which obtained from partial hydrolysis of the skin or animal bone.
Gelatin source which can be used as a base material to forms a hard shell capsule that is bone or skin of cow,
pig, and fish. Catfish is the most consumed fish in Indonesia with the average production is 1.8 million tons.
The resulting waste can be used to make gelatin as a basic component for capsule shells.This research was
aimed to process and evaluate the characteristics of hard shell capsule which made from the gelatin of catfish
dumbo (Clarias garepinus) bone waste. The process of making gelatin was done with use the acid method.
The gelatin which have been produced then used to make a hard capsule shell which was divided into 2
formulas by varying the amount of gelatin (Formula I = 25 g and formula II = 35 g of gelatin). Evaluation of
characteristics of the capsule shell which includes the dimensions of the capsule shell, volume the capsule
shell, the time of disintegration in water and acid liquid, moisture content and friability. The evaluation results
show that Formula I does not conforms the requirement for moisture content and friability, while formula II does
not conforms the requirement for moisture content.
Keyword: Hard capsule, Catfish bone, gelatin
52
Poster Presentation [PP-007]
COST OF ILLNESS STUDY OF DENGUE HAEMORRHAGIC FEVER PATIENTS ONE OF PRIVATE HOSPITALS IN YOGYAKARTA
Woro Supadmi a,b, Qorni Nadwatul Izzahb, Auliya A Suwantikaa,c, Dyah A Perwitasarib, Rizky Abdulaha,c
a Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran,
Sumedang, Indonesia
b Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia c Center of Excellence in Higher Education for Pharmaceutical Care Innovation, Universitas
Padjadjaran, Sumedang, Indonesia
ABSTRACT
Dengue Haemorrhagic Fever (DHF) caused by dengue virus, is one of high prevalence diseases in tropical
countries, such as Indonesia. It has been highlighted that prevalence diseases are strongly correlated with
high treatment costs. In particular, Yogyakarta has been reported as one of provinces with high prevalence of
DHF. The aim of this study was to estimate the cost of illness due to DHF and analyse the correlation between
the use of drugs with its cost and length of stay with total hospital administrative cost at one of private hospitals
in Yogyakarta. A cross sectional study was applied in this study by collecting patients’ medical record and
financial data, such as demographic characteristic, medication, length of stay, and treatment cost. Healthcare
perspective was applied in this study by considering 87 and 143 patients with DHF in 2015 and 2016,
respectively. Two groups of patients were taken into account in this study: (i) covered by national health
insurance (BPJS Kesehatan) and (ii) non-BPJS Kesehatan. Data related to indirect medical costs collected
from a questionnaire, which was fulfilled by 20 respondents. Spearman’s statistical test was used in this study
to conduct bivariate test. The result showed that the average costs of illness in 2015 were estimated to be
USD 175.21 (BPJS Kesehatan) and USD 266.36 (non-BPJS Kesehatan). In 2016, the average costs of illness
were estimated to be USD 243.39 (BPJS Kesehatan) and USD 274.51 (non-BPJS Kesehatan). There was a
correlation between the use of drugs with its cost at values of rho=0.527; p<0.05 in 2015, and rho=0.603;
p<0.05 in 2016. Additionally, there was also a correlation between length of stay with total hospital
administrative cost at values of rho=0.375; p<0.05 in 2015, and rho=0.562; p<0.05 in 2016. Productivity loss
due to DHF was estimated in a range of USD 70.98-709.82. It can be concluded that DHF has a relatively high
cost of illness both in direct and indirect costs.
Keywords: cost of illness, dengue hemorrhagic fever
53
Poster Presentation [PP-008]
ETHNOBOTANICAL SURVEY OF MEDICINAL PLANTS USED IN MALARIA MANAGEMENT IN SUKABUMI REGENCY, WEST JAWA PROVINCE,
INDONESIA
Ami Tjitraresmi 1), Moelyono Moektiwardoyo 1), Yasmiwar Susilawati 1),
Yoshihito Shiono 2)
1)Faculty of Pharmacy, Universitas Padjadjaran 2) Department of Food, Life, and Environmental Science, Faculty of Agriculture, Yamagata
University
ABSTRACT
Malaria, globally, is still a significant health problem because it affects a large population in the world. The
malaria endemicity level in Indonesia in 2016 shows that 48.1% of districts or cities have been certified free of
malaria, but 51.9% of districts or cities still have endemic status of malaria. Districts in West Java which are
still malaria endemic areas are Sukabumi regency, Garut regency, Tasikmalaya regency and Pangandaran
regency. West Java community, which part of Sundanese ethnic society,have hereditary knowledge of hebal
medicine which is often the basis of research in the field of pharmacy or medicine.
The aim of this study was to investigated medicinal plants used by Sundanesse society in Sukabumi regency.
Information was collected by interviewing indigenous people, using a semistructured questionnaire.
A total of 74 respondents were interviewed of which 45.9% were females and 54.1% males. This population
comprised of farmers (44.6%), housewives (37.8%) and herbal medicine sellers or entrepreneur (17.6%).
Thirty six plants species used in the treatment of malaria by Sundanese Tribe of Sukabumi District belonging
to 24 families were identified and compiled detailing information such as common and vernacular names, parts
used, methods of preparation and previous scientific reports. The most frequently cited plant species was
Carica papaya L., Sterculia oblongata R. Brown, Swietenia mahagoni Jack., and Kalanchoe pinnata L. The
most common preparation method is decoction and the route of administration is oral. The results
contributed to the conservation of empirical knowledge of medicinal plants used for the treatment of
malaria, but its scientific validation claims of anti-malarial properties is imperative.
Keywords: ethnobotany, anti-malarial, medicinal herbs, Sukabumi Regency
54
Poster Presentation [PP-009]
EFFECT OF ANTIPSYCHOTIC ATYPICAL TO BLOOD PRESSURE IN SCHIZOPHRENIA PATIENTS WHO INPATIENT IN RUMAH SAKIT JIWA PROF. DR.
SOEROJO MAGELANG
Haafizah Dania1,3, Rizky Abdulah1,4, Melisa I. Barliana2,4, Dyah A. Perwitasari3
1Departemen Farmakologi dan Farmasi Klinik, Fakultas Farmasi, Universitas Padjajaran, Sumedang,
Indonesia, 2Departemen Biologi Farmasi, Fakultas Farmasi, Universitas Padjajaran, Sumedang, Indonesia, 3Fakultas Farmasi, Universitas Ahmad Dahlan, Yogyakarta, Indonesia, 4Pusat Unggulan Riset Inovasi
Pelayanan Kefarmasian, Universitas Padjajaran, Sumedang, Indonesia
ABSTRACT
Schizophrenia ranks in the top of all mental disorders which also has a poor prognosis. Central Java Province
ranks on the top five of schizophrenia incidents in Indonesia. Antipsychotic is a main therapy for schizophrenia,
which divides into 2, atypical and typical. Atypical antipsychotics is more preferable because of the minimal
effect of extrapyramidal syndrome, but its also affect to the blood pressure. The aim of this study is to analyze
of blood pressure in schizophrenia patients pre and post the use of atypical antipsychotics in RSJ Prof. Dr.
Soerojo Magelang.
This study is an observational study which use cross sectional methods. Data was taken prospectively to figure
out the blood pressure of patients who inpatient in hospital as a ‘post’ data. Otherwise, data also was taken
retrospectively using patient’s medical records to figure out the use of drug and the blood pressure 3 months
before as a ‘pre’ data. Subject on this study is schizophrenia patients who inpatients in period of January-April
2019 in Rumah Sakit Jiwa Prof. Dr. Soerojo Magelang. Those subjects was taken using consecutive sampling
methods and achieved 106 subjects. Inclusion criterias on this study are patients who diagnosed with
schizophrenia based on Diagnostic and Statistical Manual of Mental Disorders-V (DSM V), aged about 17-55
years old, received of antipsychotic atypical therapy minimal for 3 months, and willing to follow this study by
giving an approval on informed consent letter. The exclusion criterias are patients who also receive
antidepressants and anti hypertension, had a history of cardiovascular disease and hypertension, and medical
records which is incomplete. The data was analyzed descriptive in the form of patient characteristics,
antipsychotic type, and description of patient's blood pressure pre and post therapy.
Result of this study showed that the characteristics of patients are dominated by male (78.3%), aged around
26-35 years old (39.6%),and aged around 36-45 years old (45.3%). Most of patients using antipsychotic
atypical combination (88.7%), which is the highest is combination of risperidon and clozapine (82.1%). In this
study patients who experienced a decrease in systolic blood pressure number of 43 patients, an increase in
systolic blood pressure of 57 patients, there was no change in systolic blood pressure were 6 patients, a
decrease in diastolic blood pressure number of 47 patients, an increase in diastolic blood pressure of 50
patients, there was no change in systolic blood pressure were 9 patients.
Keywords: antipsychotic atypical, schizophrenia, blood pressure
55
Poster Presentation [PP-010]
EFEK GASTROPROTEKTIF EKSTRAK ETANOL GANGGANG HIJAU (Ulva lactuca, L.)
PADA LAMBUNG TIKUS YANG DIINDUKSI ETANOL
Wahyu Widyaningsih, Siti Nurasih Afdaliah
Fakultas Farmasi Universitas Ahmad Dahlan
Jl. Prof. Dr. Soepomo, Janturan, Yogyakarta, Telp. (0274) 379418
ABSTRAK
Tukak lambung merupakan penyakit pada saluran pencernaan ditandai adanya kerusakan mukosa. Salah
satu tanaman yang berpotensi sebagai gastroprotektor adalah ganggang hijau. Tujuan penelitian ini adalah
menguji efek gastroprotektif ekstrak etanol ganggang hijau (EEGH) Ulva lactuca, L. pada lambung tikus yang
diinduksi etanol. Penelitian ini menggunakan 36 ekor tikus, dibagi kedalam 6 kelompok. Kelompok I adalah
kelompok normal. Kelompok II kontrol negatif, diinduksi etanol 96%. Kelompok III kontrol positif, diberi
pembanding ranitidin dosis 15,75 mg/kgBB. Kelompok IV, V dan VI diberi EEGH dosis bertingkat yaitu 125,
250 dan 500 mg/kgBB. Perlakuan dilakukan selama 14 hari, pada hari ke-14 tikus diinduksi etanol 96% lalu
dibedah. Data penelitian indeks tukak lambung dan rasio proteksi dianalisis dengan uji Kolmogorov-Smirnov
dan uji Levene, selanjutnya di uji menggunakan One Way ANOVA dengan taraf kepercayaan 95%. Hasil uji
statistik EEGH dosis 500 mg/kgBB dengan kontrol negatif menunjukkan adanya perbedaan signifikan dengan
nilai p 0,013 (p<0,05), sedangkan EEGH dosis 125 mg/kgBB dan 250 mg/kgBB tidak berbeda signifikan
dengan kontrol negatif. Ekstrak etanol ganggang hijau Ulva lactuca, L. dosis 500 mg/kgBB memiliki
kemampuan sebagai gastroprotektor yang efektif, dilihat dari nilai indeks tukak lambung yaitu 0,5 dan rasio
proteksi yaitu 78,94%.
Kata kunci: Ulva lactuca, L., gastroprotektor, indeks tukak, rasio proteksi, etanol.
56
Poster Presentation [PP-011]
MOLECULAR DOCKING STUDIES OF COUMARIN DERIVATIVES AGAINST ACE INHIBITORS AS ANTIHYPERTENSIVE
Riska Prasetiawati1,2, Putri Maya Indah Sari2, Benny Permana3
1) Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas
Padjadjaran, Jatinangor-Sumedang, West Java, Indonesia 2) Department of Pharmacy, Faculty of Mathematics and Natural Science, Universitas Garut, West Java,
Indonesia 3)School of Pharmacy Institut Teknologi Bandung, West Java, Indonesia
ABSTRACT
Angiostensin-I Converting Enzyme (ACE) is an enzyme that plays an important role in regulating blood
pressure.The phenolic in natural product can be effective in lowering blood pressure through inhibition and
decrease in ACE expression. In this study, the interaction between the coumarin derivative compounds with
ACE were observed using molecular docking study. The visualized using AutoDock Tools and Discovery
Studio Visualizer. The captopril (1UZF) has a ∆G of -6.84 kcal/mol and lisinopril (1O86) has ∆G of -8.01kcal/mol
are used as comparative ligands. The results showed that the coumarin derivative, warfarin, has a fairly good
∆G of -8.07kcal/mol which is docked to 1UZF while for the 1O86 has a ∆G -8.37 kcal/mol. For libanotin
docked to 1UZF has a ∆G -7.38 kcal/mol while for the 1O86 it has a ∆G of -8.76 kcal/mol. The Pre-ADME
test in the form of absorption parameters and distribution of warfarin compounds and libanotin are good with
non-mutagenic and not carcinogenic result for toxicity. This indicates that the coumarin derivative has the
potential as an antihypertensive agent.
Keywords: Hypertension, coumarin derivatives, angiostensin converting enzyme, molecular docking
57
Poster Presentation [PP-012]
FORMULATION OF LIQUID FACIAL SOAP OF ETHANOL EXTRACT OF BAY LEAF’S (Syzygium polyantum W.) AS ANTIACNE
Aji Najihudin1), Dolih Ghozali2), Kartika Sari1)
1) FMIPA Universitas Garut 2) Fakultas Farmasi Universitas Padjadjaran
ABSTRACT
The Formulation of liquid face soap of ethanol extract preparation formula from bay leaves (Syzygium
polyantum W.) as antiacne had been done. The purpose of this research was to formulate bay leaf’s extract
ethanol liquid soap preparation and to prove that the preparation made had an antibacterial activity towards
Propionibacterium acnes. The examination of the ethanol extract was done by gel diffusion perforation
technique method, and minimum inhibitory concentration was determined as of 4%. Basis formulation of the
liquid soap was made by varying sodium lauryl sulfate in 5, 10 and 15% concentration, where the 15% sodium
lauryl sulfate concentration was relatively stable for 28 days storage. Face’s liquid soap preparation formulation
had been done with the variation of extract ethanol as of 4, 6 and 8%. The result of physical evaluation showed
that the liquid face soap was relatively stable observed from organoleptic, pH, viscosity, amount and stability
of foam, spreading, density, and freeze and thaw in the storage process. The result of preparation’s activity
test showed that liquid face soap F1, F2, and F3 had antibacterial activity toward Propionibacterium acnes
with inhibitor diameter as of 12.7775 mm, 13.0213 mm, and 13.0213 mm. The irritation test showed that the
liquid face soap didn’t irritate, which means, the liquid was safe to be used as tropical preparation.
Keywords: liquid face soap, bay leaf, antiacne.
58
Poster Presentation [PP-013]
SUPEROXIDE DISMUTASE ACTIVITY AND HISTOLOGY ANALYSIS OF MALE RATS AFTER ADMINISTRATION OF NANOPARTICEL OF GINGER TORCH FLOWER
Ade Yeni Aprillia1),Tresna Lestari1), Tita Nofianti1, Lilis Tuslinah1 and Ruswanto Ruswanto1
1) Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada [email protected]
ABSTRACT
Superoxide dismutase (SOD) is an endogenous antioxidant that plays role on protecting cells from oxidative
stress. Some natural substances could enhance SOD activity and prevent cell damage due to free radicals.
Ginger torch flower has been previously studied for its antioxidant activity. Nanoparticle formulation of ginger
torch flower extract is expected to increase its absorptivity in the biological membrane, thus increasing the
sharpness of the action. Ginger torch flower extract was formulated into nanoparticle using poloxamer 1, 3 and
5%. The nanoparticle was then characterized for its particle size, polydispersity index, zeta potential,
entrapment efficiency and morphological form by SEM. The result shows that nanoparticle formulations have
particle size 134.7-193.1 nm, polydispersity index less than 0.5 for all formulas, zeta potential -41.0 - (-24.3)
mV and entrapment efficiency 89.93-97.99 against flavonoid content with a soft surface and spherical form of
particles. The administration of 2 mg/200 g BW of nanoparticle formulation could enhance superoxide
dismutase activity by 1.636 U/ml compared the extract 1.374 U/ml. Both extract and nanoparticle increase
superoxide dismutase activity significantly distinguished to negative control 0,904 U/ml. The histology assay
against kidney and liver shows nanoparticle preparation could repair tissue damage on the organ profile.
Keywords: antioxidant; superoxide dismutase; nanoparticle, ginger torch flower
59
Poster Presentation [PP-014]
THE ACTIVITY OF MORINGA (Moringa oleifera) LEAVES AND PAPAYA (Carica
papaya L.) FRUITS EXTRACTS AS ELASTASE ENZYM INHIBITOR IN VARIATIONS
CONCENTRATION OF ACETONE
Dewi Ramonah1), Nining Sugihartini2), Any Guntarti2)
1)Pascasarjana program of Pharmacy, Ahmad Dahlan University, Yogyakarta 2) Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta
ABSTRACT
Sun exposure on the skin cause the formation of Reactive Oxygen Species (ROS), which can trigger an
increasing of elastase enzyme activity so that it accelerating skin aging. This condition can be prevented by
the presence of antioxidant compounds in moringa (Moringa oleifera) leaves and papaya (Carica papaya L.)
fruits. The purpose of this study was to determine inhibitory activity of elastase enzyme by moringa leaves and
papaya fruits extract at various concentrations of acetone. Acetone extract of moringa leaves and papaya fruits
was obtained by maceration method using variations concentrations of acetone: 50%, 70% and 96%. Both
extracts were tested for inhibitory activity of elastase enzymes in vitro so that IC50 values were obtained. The
results showed the IC50 value acetone extract of Moringa leaves concentrations 50%, 70%, and 96% are
11392.02 μg/ml; 8025.39 μg/ml; and 6373.81 μg/ml, respectively. The IC50 value acetone extract of papaya
fruits concentration 50%, 70% and 96% are 11219.33 μg/ml; 7432.54 μg/ml; and 6440.17 μg/ml, respectively.
Based on the data, the extract which has the most optimal inhibitory activity of elastase enzyme is extract of
Moringa leaves with acetone 96% solvent.
Keyword: Moringa Leaves, Papaya Fruit, Concentration Variations, Acetone, Elastase Enzyme
60
Poster Presentation [PP-015]
ACTIVITY OF ELASTASE ENZYME INHIBITOR OF KELOR LEAF EXTRACT (Moringa oleifera) AND PEPAYA FRUIT EXTRACT (Carica papaya L.) IN VARIATIONS OF
METHANOL CONCENTRATION
Mohamad Usman Nur1), Nining Sugihartini2), Sapto Yuliani2)
1)Pascasarjana of Pharmacy, Ahmad Dahlan University, Yogyakarta 2)Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta
ABSTRACT
Methanol extract of moringa leaves and papaya fruits which contained β-carotene, flavonoids and phenolics
compounds have high antioxidant activity so that they can be used to inhibit activity of elastase enzyme which
plays a role in the aging process of the skin. This study aims to determine inhibitory activity of elastase enzyme
by moringa leaves and papaya fruits extract at various concentrations of methanol. In this study, variations in
the concentration of methanol 50%, 70%, and 96% were used to moringa leaf and papaya fruit extracts by
maceration method. The extracts were tested by In-Vitro inhibitory activity of elastase enzyme. The results of
the inhibitory activity of elastase enzyme in moringa leaves extract with methanol solvents 50%, 70%, and
96% had IC50 values were 9453.38 μg/ml; 6604.70 μg/ml; 12346.44 μg/ml respectively. IC50 values of papaya
fruits extract were 5995.31 μg/ml; 9046.25 μg/ml; 11571.54 μg/ml respectively. Based on the test results it can
be concluded that the most effective solvent as an inhibitor of elastase was methanol papaya fruits extract at
a concentration of 50%.
Keywords: Moringa oleifera, Carica papaya, methanol, elastase enzyme
61
Poster Presentation [PP-016]
PREPARATION AND CHARACTERIZATION OF CALCIUM CARBONATE FROM EGGSHELL (Gallus gallus Domesticus)
Dadih Supriadi2), Tiana Milanda1), Anis Yohana C.1), Marline Abdassah1),
1) Faculty of Pharmacy, Padjadjaran University, Jatinangor, Indonesia 2) Bandung School of Pharmacy, Bandung, Indonesia.
ABSTRACT
Calcium carbonate is widely used in the pharmaceutical field as excipients and therapeutic agents. Calcium
carbonate can be obtained from limestone, chalk, marble and dolomite. Other alternative is from eggshell.
Calcium carbonate source from eggshell has several advantages including higher calcium carbonate content,
fewer contaminants metal limit, and more brittle. Therefore, in this study, calcium carbonate has been isolated
from eggshells which is expected to meet the requirements of Indonesian Pharmacopoeia (fifth edition).
Calcium carbonate were isolated from eggshells by mechanically and physically organic separation. The
quality of calcium carbonate were examined according to the Indonesian Pharmacopoeia including loss on
drying; acid-insoluble substance, magnesium and alkali salt; limit of arsenic, lead, iron, mercury, heavy metal,
and barium. The results showed that calcium carbonate from eggshell fulfilled the requirements. This isolation
produces 98.5 ± 0.5 % of calcium carbonate. Additional physicochemical properties of calcium carbonate was
characterized including particle size analyzer, FTIR and XRD and compared with commercial calcium
carbonate. The results showed that calcium carbonate powder had an average size of 21 ± 1.0 µm, while that
of commercial was 8 ± 1.3 µm. The results showed that calcium carbonate similar XRD patterns with that from
commercial. Based on result, calcium carbonate had 99% similarity level with calcium carbonate reference
(data base from FTIR instrument).
Keywords: Eggshell, Calcium carbonate powders, Pharmaceutical grade
62
Poster Presentation [PP-017]
INHIBITION OF ELASTASE ENZYM BY ETHANOL EXTRACT OF MORINGA LEAVES (Moringa oleifera L.) AND PAPAYA FRUITS (Carica papaya L.) WITH VARIATION
CONSENTRATIONS OF SOLVENT
Ristia Aprisida Rahmawati1, Nining Sugihartini2, Sapto Yuliani2
1)Pascasarjana program of Pharmacy, Ahmad Dahlan University, Yogyakarta 2) Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta
ABSTRACT
Ethanol extract of moringa leaves and papaya fruits have antioxidant activity which can inhibit activity of
elastase enzyme so that it can be used as antiaging. This study aims to determine the effect of variations in
ethanol concentration on the process of extracting moringa leaves and papaya fruits in inhibitory activity of
elastase enzyme. The samples ethanol extract of moringa leaves and papaya fruits was made with variation
of ethanol 50%, 70%, and 96% by maceration method. The extract was tested for inhibitory activity of elastase
enzyme to obtain IC50 value In Vitro. The test results showed IC50 value of the ethanol extract of moringa
leaves with a concentration of 50%, 70%, and 96% were 6788.91; 10163.14; 11999.86, respectively. IC50 value
in the ethanol extract of papaya fruits were 10626.05; 6676.88; 11775.09, respectively. Based on the test
results, the best solvent in inhibitory activity of elastase enzyme is extract ethanol papaya as concenttration
70%.
Keyword: moringa leaves (Moringa oleifera L.), papaya fruits (Carica papaya L.), elastase enzym
63
Poster Presentation [PP-018]
MIXTURE OPTIMIZATION OF PAPAYA FRUIT EXTRACTS (Carica papaya L. Extract) AND MORINGGA LEAVE (Moringa oleifera Extracts) AS SUNSCREEN
Fathul Alimsyah (1), Nining Sugihartini (2), Hari Susanti (2)
1)Master of Pharmacy, Ahmad Dahlan University, Yogyakarta 2)Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta
ABSTRACT
In previous research showed that the ethanol extract of Moringa leaves and ethanol extract of papaya fruit had
antioxidant activity and sunscreen. Research on the mixed activity of the two extracts has not been carried
out. The aim of this study was to determine the composition of the mixture of 70% ethanol extract of papaya
fruit and 50% ethanol extract of Moringa leaves on antioxidant activity and Sun protection factor (SPF) values.
The research was conducted based on the concept of Simplex Lattice Design (SLD). Based on this method,
there are three test formulas consisting of 100% Moringa leaf ethanol extract (FI), 50% mixture of Moringa leaf
ethanol extract and 50% papaya ethanol extract (FII), 100% papaya fruit ethanol extract (FIII). Each formula
was determined by antioxidant activity with IC50% parameters using the DPPH method and SPF value with a
spectrophotometer. Based on the SLD concept, the equations for IC50% values are y = 245 (A) +51 (B) +232
(A) (B), While the SPF value equation is y = 34 (A) +31 (B) +2 (A) (B) .Based on the equation, the largest total
R value is obtained at 1 at an increased concentration of 90:10 and Moringa leaf extract has an effect for
antioxidant activity and SPF.The optimal composition is 90% Papaya extract and 10% Moringga extract.
Keywords: Papaya, Moringga, Antioxidant, Sun Protection Factor, Simplex Lattice Design
64
Poster Presentation [PP-019]
ANTIOXIDANT AND ANTIAGING ACTIVITY OF PEPAYA FRUIT ETHANOL EXTRACTS (Carica papaya L.)
Aditya Noviadi Rakhmatullah1), Nining Sugihartini2), Hari Susanti2)
1) Pascasarjana Program of Pharmacy, Ahmad Dahlan University, Yogyakarta 2)Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta
ABSTRACT
Antioxidants has activity as sunscreen and as antiaging. One of the fruits that has antioxidant and antiaging
activity is papaya (Carica papaya L.). The purpose of this study was to determine the antioxidant activity and
antiaging of ethanol extract of papaya fruit. The ethanol extract of papaya fruit was obtained with maceration
method by using 70% ethanol. The extract was tested for antioxidant activity with variations in extract
concentrations of 150 ppm, 175 ppm, 200 ppm, 225 ppm, and 250 ppm, inhibition activity of elastase enzyme
with variations in concentrations of 500 ppm, 1000 ppm, 1500 ppm, 2500 ppm, 3000 ppm, 4000 ppm and 5000
ppm and determination of the value of SPF (Sun Protection Factor). The results showed the best antioxidant
activity of the ethanol extract of papaya fruit at a concentration of 250 ppm which was equal to 33.024%, the
best inhibition activity of elastase enzyme in ethanol extract of papaya fruit was at a concentration of 1500
ppm which was equal to 29.45%, and the SPF value of papaya ethanol extract was 36.58. Based on the data
it can be concluded that the ethanol extract of papaya fruit has good activity as a sunscreen.
Keywords: Papaya, Ethanol, Antioxidants, Elastase Enzymes, SPF
65
Poster Presentation [PP-020]
EFFECT OF BRIEF COUNSELING-5A MODIFICATION AND SMS MOTIVATION OF RELIGIUSITY ON INCREASING TREATMENT BEHAVIOR AND COMPLIANCE OF
PATIENTS DIABETES MELLITUS
Ginanjar Zukhruf Saputri1, Akrom2, Okta Muthia S2
1)Pharmacy faculty, Universitas Ahmad Dahlan, Yogyakarta, Indonesia 2)Pharmacy program, Faculty of Mathematics and Natural Sciences, Lambung Mangkurat University
Banjarbaru, Indonesia
ABSTRACT
Compliance can affects achievement of therapeutic target of Diabetes Mellitus (DM) in the form of
controlling blood glucose levels. It takes an understanding of knowledge related to DM management to improve
adherence behavioral treatment in DM patients. The role of Pharmacists for counseling is known to have a
positive effect on compliance, but there are still many media for counseling and reminder. This study aims to
determine the effect of brief counseling 5A modification accompanied by a reminder-motivational message
(SMS) with a religiosity approach to treatment behavior and adherence to outpatient diabetes mellitus (DM)
patients at Jetis 1 Bantul Health Center, Yogyakarta.
The study used a quasi-experimental design pre-test - post-test control group design. Data collection
was carried out during the period from May to August 2018 prospectively in outpatients at the Jetis 1 Health
Center, Bantul, Yogyakarta. A total of 72 patients fulfilled the inclusion criteria and filled out Informed consent
divided into 2 groups, namely the treatment group (N=36 patients) and the control group (N=36 patients). The
exclusion criteria are pregnant and deaf. Data collection was conducted by interview using a treatment
behavior questionnaire and MARS compliance questionnaire. Statistical analysis used the Wilcoxon test to
assess the level of treatment behavior and the level of patient compliance.
The results of the study showed that brief counseling 5A modification with a short message of religiosity
motivation can improve the stages of behavior change in DM patients. Increased scores for treatment behavior
were (Δ 2.76 ± 1.59) and increased medication adherence (Δ 3.44 ± 3.45). Based on the results of this study,
it can be concluded that the provision of brief counseling 5A modification by pharmacists along with a reminder-
motivational message can improve treatment behavior and adherence to DM patient therapy.
Keywords: diabetes, counseling, short messages, treatment behavior, compliance
66
Poster Presentation [PP-021]
KAHAR METHOD: A NOVEL CALCULATION METHOD OF TONICITY ADJUSTMENT
Abd. Kakhar Umar1,*), Nasrul Wathoni1), Aliya Nur Hasanah2), Insan Sunan Kurniawan Syah1)
1) Department of Pharmaceutics and Pharmacetical Technology, Faculty of Pharmacy, Universitas
Padjadjaran, Sumedang, 45363, Indonesia. 2) Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas
Padjadjaran, Sumedang, 45363, Indonesia
ABSTRACT
Hypertonic and hypotonic conditions in pharmaceutical preparations decrease the drug’s absorption and
bioavailability. In addition, it can cause a tissue damage. There are several calculation methods to regulate
hypotonic preparations. However, there are no methods that can be used to regulate hypertonic preparations
without causing dose dividing problem. Therefore, this study aimed to develop a calculation that can solve the
problems of the tonicity preparations, especially for hypertonic preparations through reducing the levels of
additional ingredients. Kahar method was succesfully obtained and then evaluated by resolving the problems
in 42 sterile formula preparations and compared with White-Vincent method, Cyroscopic method, Equivalent
NaCl method, and Milliequivalent method through the analysis of its similarity and reliability. The results of
similarity analysis between Kahar method and other methods showed a good similarity values with more than
0.880. The highest similarity was on Kahar and Cyroscopic methods with similarity value is 1. The reliability
analysis obtained a very good result with Cronbach alpha values was 0.990. These results suggest that Kahar
method provides a reliable equation with complete and efficient solution to hypotonic and hypertonic problems.
Keywords: Tonicity Adjustment, Kahar Method, Hypertonic Preparations, Content Adjustment.
67
Poster Presentation [PP-022]
ANTIMICROBIAL ACTIVITY OF EXTRACT AND FRACTIONS OF RED ALGAE (Gracilaria sp.)
Sulistiyaningsih1, Raden Bayu Indradi1, Yoppi Iskandar1, Eli Halimah2, Amelia Herlambang3, Amirah Yasmin T3, Dina Sofa I3, Ade Zuhrotun1
1Biological Pharmacy Department, 2Pharmacology and Clinical Pharmacy Department, 3Undergraduate
Pharmacy Study Program
Faculty of Pharmacy Universitas Padjadjaran
Jl. Raya Bandung Sumedang KM 21, Jatinangor 45363 1Department of Biological Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia 45363
2Faculty of Pharmacy, Universitas Padjadjaran, Indonesia 45363
ABSTRACT
Red algae (Gracilaria sp.) is one of the marine biota in Indonesia which has various bioactive secondary
metabolites, making it a potential source of bioactive compounds that are very beneficial for the development
of the pharmaceutical industry such as antibacterial and antifungal. Algae from Indonesian waters are found
to contain active compounds, especially from the class of alkaloid compounds, flavonoids, and tannins. The
number of cases of infection caused by bacteria and fungus is the background of research on the potential of
red algae extracts and fractions as antibacterial and antifungus. The study was conducted through testing the
antibacterial and antifungus activity of ethanol extract and fraction and determining the Minimum Inhibition
Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the most active fraction. The method
used is agar diffusion method and determination of MIC and MBC using microdilution method. The results
showed that the antibacterial and antifungus activity of red algae (Gracilaria sp.) was only found in the ethyl
acetate fraction with the most active inhibitory zone by ethyl acetate fraction which was 15,14 mm against the
bacterium Staphylococcus aureus clinical isolate, 14,67 mm against the bacterium Pseudomonas aeruginosa
clinical isolate, 15,03 mm against the bacterium Staphylococcus epidermidis clinical isolate, and 11,08 mm
against the Candida albicans. The value of the MIC and MBC generated from the most active fraction was in
the range of 25% -50% (b/v).
Keywords: Red Algae, Antibacterial, Antifungus, MIC, MBC, Staphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus epidermidis ,Candida albicans.
68
Poster Presentation [PP-023]
PERCEPTION ABOUT TRADITIONAL HERB FOR HYPERTENSION PATIENTS IN BANYUMAS
Aulia Rahman1), Dyah Aryani Perwitasari1), Kintoko1), Suwijiyo Pramono2)
1)Pasca Sarjana Farmasi Universitas Ahmad Dahlan Yogyakarta
2)Fakultas Farmasi Universitas Gadjah Mada Yogyakarta
ABSTRACT
Hypertension was one of the disease which can be treated by traditional herb. The used of traditional herb
influence by characteristic and perception of patients. The aim of this research is to investigate the patients’
perception about traditional herb of hypertension and to understand the relationship between characteristics
and perceptions, Subjects were member of PROLANIS in Banyumas District. Cross sectional study was used
as design with purposive random sampling. Perceptions measured with Likert scale Questioner from strong
disagree to strong agree (1-5 scale). There were 340 subjects participated in this study. Majority of
respondents was female (56,8%) and between 45 – 60 years old (52,4%). Majority of respondent were agree
(perception score above 60%) with safety, effectiveness, availability and acceptability perception. Strong agree
(perception score above 80%) with price perception of traditional herb. Type of Occupation had significant
relationship with perception of safety, level of income had significance relationship with acceptability and price
of traditional herb (p<0.05). No significance relationship (p>0,05) between gender, age, and level of education
with perception of traditional herb.
Keyword: Hypertension, Perception, Traditional herb, Prolanis
69
Poster Presentation [PP-024]
ASSESSMENT OF THE EXTRACTION METHOD ON PHENOLIC COMPOUND, FLAVONOID COMPOUND AND ANTIOXIDANT ACTIVITY IN RUBUS ROSAEFOLIUS
Yesi Desmiaty1,2), Muhammad Hanafi1,3), Fadlina Chany Saputri2), Rini Prastiwi4), Berna Elya2)*
1Faculty of Pharmacy, University of Pancasila, Jakarta, 12640, INDONESIA
2Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, INDONESIA 3Chemistry Research Centre, Indonesian Institute of Sciences (LIPI), Serpong, 15314, INDONESIA
4Faculty of Pharmacy, Universitas Muhammadiyah Prof. Dr. Hamka, Jakarta, INDONESIA
ABSTRACT
Introduction: Rubus rosaefolius can be found wildly in the mountainous area of Java, Indonesia. Previous
research shows that this plant has a strong antioxidant activity. Extraction methods will affect the content of
the compounds present. In this study, an evaluation of extraction methods of leaves, stem and fruit with
parameters: yield, phenolic and flavonoid content, and antioxidant activity, has been done. Method: The
leaves, fruit, and stem of R. rosaefolius were extracted by maceration, reflux and soxhlet using methanol
solvent. The total phenolic content (TPC) level was determined by Folin-Ciocalteu method, the assay of total
flavonoid content (TFC) using aluminium chloride reagent, and the antioxidant activity was tested using DPPH
scavenger method. Result: The leaves extract made by reflux gave the most rendement. Assay of TPC and
TFC showed the same pattern which the highest content was leaves extract (reflux). The fruit extract (reflux)
has the best antioxidant activity. Using Pearson’s test showed there was a strong correlation between TPC-
TFC (reflux) with r=0.994 as well as antioxidant activity-TPC (Soxhlet) with r=0.998. Conclusion: These
finding suggested that the reflux and soxhlet method were the efficient methods for extracting the polyphenol,
flavonoid and antioxidant content, as well as the leaves and fruit have the potential to be used as a natural
antioxidative.
Keyword: Rubus rosaefolius, extraction method, phenolic content, antioxidant
70
Poster Presentation [PP-025]
ANALISIS KADAR FENOLIK TOTAL DAN FLAVONOID TOTAL EKSTRAK DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav.) DENGAN METODE
SPEKTROFOTOMETER
1)Irma Erika Herawati, Lisna Dewi
1)Jurusan Farmasi, FMIPA Universitas Al-Ghifari, Bandung, Jawa Barat
ABSTRAK
Flavonoid sebagai salah satu kelompok senyawa fenolik yang banyak terdapat pada jaringan tanaman dapat
berperan sebagai antioksidan. Penelitian ini bertujuan untuk menentukan kadar fenolik total dan flavonoid total
yang terkandung dalam daun sirih merah (Piper crocatum Ruiz & Pav.) yang dapat dimanfaatkan sebagai
obat. Secara tradisional sirih merah sering digunakan sebagai obat penyakit seperti sariawan, sakit gigi, obat
kumur, mencegah bau mulut serta menghilangkan bau badan , antidiabetik, antikanker, antiseptik, dan
antiinflamasi. Penelitian meliputi pengumpulan bahan simplisia sirih merah yang diambil dari daerah Lembang,
determinasi simplisia, dan ekstraksi simplisia. Ekstraksi daun sirih merah (Piper crocatum) menggunakan
metode maserasi dengan pelarut etanol 70 % selama 3 x 24 jam , penentuan kadar air, susut pengeringan,
identifikasi fenolik total dan flavonoid total dengan metode reaksi warna, analisis kadar fenolik total
menggunakan pereaksi Folin-Ciocalteau. Hasil penelitian menunjukkan bahwa kadar fenolik total sirih merah
sebesar 0,658 mg/g ekstrak dan hasil flavonoid total sebesar 0,03677 mg/g ekstrak setara dengan flavonoid
Rutin.
Kata kunci: Sirih Merah (Piper crocatum Ruiz & Pav.), fenolik total, flavonoid total
71
Poster Presentation [PP-026]
COMPARISON NEPHROPROTECTION EFFECT OF CURCUMIN AND CURCUMIN NANOPARTICLES ON CISPLATIN INDUCED RATS
Ni Made Dwi Sandhiutami1,2*), Wawaimuli Arozal3), Melva Louisa3), Deni Rahmat1), Tjoeng Mandy1)
1 Faculty of Pharmacy, University of Pancasila, Jakarta, Indonesia 2 Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
3Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta,
Indonesia
ABSTRACT
Cisplatin is a first-line chemotherapeutic agent for various solid tumors including ovarian and breast cancer.
Although it has been proven effective as an antineoplastic agent, its use is clinically limited because of its side
effects specifically nephrotoxicity. The aim of this study was to utilize curcumin as a renoprotector against
cisplatin nephrotoxicity formulated in the form of nanoparticles using ionic gelation method. Cisplatin
nephrotoxicity was investigated through several parameters, namely serum creatinine, blood urea nitrogen,
serum albumin, kidney weight ratio, and histopathology. Twenty-four white rats (Sprague Dawley) were divided
into 4 groups, namely the normal group, the negative control group given cisplatin at a dose of 7 mg/kgBW i.p,
the cisplatin + curcumin group, and the cisplatin + curcumin nanoparticles group. Curcumin and curcumin
nanoparticles were administered at a dose of 100 mg/kgBW for 9 days before cisplatin administration. Serum
and kidneys are then taken 48 hours after cisplatin administration. The results showed that the cisplatin group
have a significant increase in creatinine, blood urea nitrogen and kidney weight ratio, and a significant
decrease in serum albumin. The administration of curcumin and curcumin nanoparticles can significantly
reduce serum creatinine, blood urea nitrogen, and kidney weight ratio and increase albumin when compared
to the cisplatin group. Nanocurcumin showed a more significant improvement compared to the group given
curcumin. Histopathological examination showed degeneration and necrosis in the cisplatin group whereas
the curcumin and curcumin nanoparticles groups showed normal histopathological features, which confirmed
the effect of renoprotection of curcumin nanoparticles on cisplatin
Keyword: Curcumin nanoparticles, cisplatin, nephrotoxicity, renoprotection
72
Poster Presentation [PP-027]
NANOPARTIKEL EKSTRAK KULIT BATANG KAYU MANIS SEBAGAI TERAPI ALTERNATIF ANTIDIABETES
Mitri Asyari1), Erni Rustiani2), dan Sri Wardatun3)
Program Studi Farmasi, FMIPA Universitas Pakuan, Bogor [email protected]
ABSTRAK
Nanopartikel adalah partikel koloid dengan diameter berkisar dari 10-1000 nm. Pembuatan nanopartikel dapat
menggunakan metode gelasi ionik. Pemanfaatan kulit batang kayu manis sebagai obat masih terbatas, hal ini
karena senyawa bioaktif kulit batang kayu manis terutama asam sinamat memiliki kelarutan yang rendah
dalam air, yaitu 0,5 gram per liter. Asam sinamat merupakan senyawa golongan fenol yang terdapat di dalam
kulit batang kayu manis yang memiliki potensi sebagai antidiabetes. Pengecilan ukuran partikel akan
meningkatan luas permukaan yang menyebabkan kelarutan dan penyerapan dalam usus meningkat.
Penelitian ini bertujuan untuk memformulasikan ekstrak kulit batang kayu manis dalam sistem
penghantaran nanopartikel dan menentukan formula terbaik. Pembuatan nanopartikel sebanyak 3 formula
dengan variasi konsentrasi natrium alginat 0,05%, 0,1%, 0,15% dan bahan tambahan kalsium klorida 0,02%
menggunakan metode gelasi ionik. Evaluasi nanopartikel meliputi ukuran partikel, indeks polidispersitas, dan
potensial zeta menggunakan Zetasizer, penetapan morfologi partikel menggunakan scanning electrone
microscopy dan efisiensi penjerapan menggunakan Spektrofotometer UV-Vis.
Hasil pengujian mutu nanopartikel ekstrak kulit batang kayu manis berupa serbuk halus berwarna
coklat kemerahan. Formula 3 dengan konsentrasi natrium alginat 0,15% dipilih sebagai formula terbaik,
dengan ukuran partikel 303,5 nm, indeks polidispersitas 0,320, zeta potensial -32,7 mV, serta efisiensi
penjerapannya sebesar 92,73% dan bentuk morfologi permukaan nanopartikel yang kurang sferis.
Kata kunci: Nanopartikel, Kulit Batang Kayu Manis, Metode Gelasi Ionik, Antidiabetes.
73
Poster Presentation [PP-028]
HERBAL CAIR CAMPURAN EKSTRAK DAUN TORBANGUN DAN DAGING BUAH ASAM JAWA SEBAGAI ANTIDIABETES
Utari1) dan Erni Rustiani2)
Program Studi Farmasi, FMIPA Universitas Pakuan, Bogor [email protected]
ABSTRAK
Daun torbangun (Coleus amboinicus) pada dosis 620mg/KgBB dan buah asam jawa (Tamarindus indica) pada
dosis 300mg/KgBB efektif menurunkan kadar glukosa dalam darah dengan mekanisme kerja mengurangi
stress oksidatif dalam tubuh. Kandungan senyawa flavonoid yang terdapat pada kedua tanaman tersebut
diduga berperan sebagai antidiabetes Karena adanya kesamaan khasiat dari kedua tanaman maka dibuatlah
kombinasi ekstrak daun torbangun dan daging buah asam jawa dalam bentuk sediaan herbal cair.
Herbal cair pada penelitian ini ditujukan untuk penggunaan secara oral sehingga dapat diberikan
penambahan bahan pengaroma, pemanis, atau pewarna yang larut dalam air untuk meningkatkan mutu
sediaan. Sediaan herbal cair dibuat dalam 4 formula berdasarkan perbedaan jenis pemanis, hasil uji kesukaan
menunjukkan bahwa herbal cair dengan pemanis gula aren yang paling disukai oleh panelis. Formula terpilih
dari uji kesukaan dilakukan pengujian mutu meliputi: uji stabilita pada tiga suhu berbeda dalam kurun waktu 8
minggu dengan parameter pengukuran pH, viskositas, bobot jenis, uji organoleptik, penetapan kadar flavonoid
dan pengujian cemaran mikroba.
Hasil uji stabilita menunjukkan adanya perubahan mutu dan penurunan kadar flavonoid pada sediaan
herbal cair setiap minggunya. Berdasarkan data penurunan kadar flavonoid pada uji stabilita diperoleh hasil
prediksi umur simpan sediaan herbal cair yaitu selama 8 minggu pada suhu ruang.
Kata kunci: Herbal Cair, Daun Torbangun, Buah Asam Jawa, Antidiabetes, Uji Stabilitas.
74
Poster Presentation [PP-029]
CHARACTERISTICS OF YOGURT FROM COW MILK, SWEET-CORN MILK, AND MIXTURE OF 50% COW MILK AND 50% SWEET-CORN MILIK
ERLINDHA GANGGA1, TIA RAHAYU1
1Faculty of Pharmacy, Pancasila University, Indonesia
ABSTRACT
Sweet-corn milk is the latest innovation with unique flavor and has some advantages over cow's milk. Sweet-
corn milk can restore energy, maintain healthy liver, stomach, intestine and is also cholesterol-free drinks
with a high fiber content which can facilitate digestion. Moreover, sweet-corn milk has a lower sugar content
than cow's milk and is suitable for diet. Yogurt is made by pasteurization and fermentation of milk. Sweet-
corn milk yogurt can be used as a healthy drink and probiotic. In this research, the cow milk, sweet -
corn milk, and the mixture of both (50% cow’s milk and 50% sweet -corn milk) is pasteurized and
fermented using bacteria starter Lactobacillus bulgaricus and Streptococcus thermophillus. This study aims
to compare the characteristics of yogurt made from cow's milk, sweet-corn milk, and mixtures of both milks.
The results obtained for cow's milk yogurt, corn milk yogurt, mix yogurt are respectively: fat content 2,40%,
0,62%, and 1,36%; protein 2,76%, 1,00%, and 1,42%; ash content 0,70%, 0,67%, and 0,69%; lactic acid
content 0,62%, 0,49%, and 0,56%; total solids not fat 15,72%, 14,62%, and 15,30%; pH 4,55, 4,27, and 4,30.
Results for Colliform contamination, Escherichia coli and Salmonella is negative or no bacteria growth. In
summary, all three types of yogurts are safe to consume, with the best one is the yogurt made from mixture
of 50% cow’s milk and 50% sweet-corn milk.
Keywords: pasteurization, yogurt, Lactobacillus bulgaricus, Streptococcus thermophillus
75
Poster Presentation [PP-031]
APPLICATION OF HIGH PERFORMANCE LIQUID CHROMATOGRAPHY IN METABOLISM STUDI OF ISONIAZID BY N-ACETYLTRANSFERASE-2
Novi Yantih1, Yahdiana Harahap2, Wahono Sumaryono1, Rianto Setiabudy3, Lestari Rahayu1
1Fakultas Farmasi Universitas Pancasila 2Fakultas Farmasi Universitas Indonesia
3Fakultas Kedokteran Universitas Indonesia
[email protected], 08129624502
ABSTRACT Background: Isoniazid (INH) is metabolized to form acetyloniazid (AcINH) by the enzyme N-
acetyltransferase-2 (NAT-2). The emphasis in the study of drug metabolism in the human is the availability of
sensitive bioanalysis methods to determine drug levels and their metabolites. INH and AcINH levels can be
determined by high performance liquid chromatography (HPLC). Objective: The purpose of this study was to
apply the HPLC method for the metabolism study of INH by NAT-2. Method: The HPLC method had been
validated. INH metabolism was studied based on the ratio of AcINH and INH levels in human plasma. Plasma
was sampled at the 3rd hour after INH administration. If the ratio is less than 1.5 it is stated that slowly
metabolism. Results: The sensitivity of HPLC to determine INH and AcINH levels were 0.1258 and
0.1188μg/mL, respectively. This method can determine INH and AcINH in plasma samples. The range of their
levels were 0.1345-1.33236 and 0.1231-2.0023μg/mL, respectively. There are 41 healthy Indonesians as
subjects. The results showed that 80.49% of subjects had slow INH metabolism. Conclusion: The reverse
phase HPLC method used has sufficient sensitivity to be applied for the determination of INH and AcINH in
the metabolism study of INH by NAT-2.
Keywords: Isoniazid, Acetylisoniazid, HPLC.
76
Poster Presentation [PP-032]
ETHANOL EXTRACT OF Moringa oleifera Lam. LEAF INDUCED EXPRESSION OF mRNA GLUT-4 AND GLUCOSE UPTAKE IN 3T3-L1 CELLS
Dian Ratih Laksmitawati1), Yati Sumiyati1), Umi Marwati1), Mutiara Putri1), Wahyu Widowati2,3 )
1)Pharmacy Faculty Universitas Pancasila, Jakarta 2) Medicinal Faculty Maranatha Christian University, Bandung, Jawa Barat
3)Biomolecular and Biomedical Research Centre Aretha Medika Utama, Bandung Jawa Barat
ABSTRACT
Moringa oleifera L. has antidiabetic effect but study on its cellular and molecular effects has not been explored
yet. The aims to this study is to examine the effect of Moringa oleifera leaf ethanol extract (MEE) on GLUT4
expression and glucose uptake in cells 3T3-L1. The 3T3-L1 cells were differentiated into adipocyte by adding
3-Isobutyl-1-methylxanthine, deksametason and insulin. The effect of MEE was observed by adding the extract
to differentiated cells. mRNA in harvested cell were isolated and its cDNA were analyzed using the qRT-PCR.
Meanwhile, glucose uptake was analyzed using colorimetric method by 2-deoxyglucose (2-DG) analogues and
measured at λ 412 nm. All treatments are compared with cell control and quercetin. The results showed that
the mRNA levels of GLUT4 of 10 g / mL and 50 g / mL of MEE was 87.22 % and 88.99 % respectively
compared to cell control. Meanwhile, 2DG-uptake value are 15.05 M and 26.23 M.. Moringa oleifera L. has
antidiabetic effect by increase Glut4 mRNA expression and glucose uptake in adipocyte 3T3L1 cells. Although
its effect is lower compare to quercetin, Moringa oleifera has still prospect to develop as antidiabetic
substances.
Keywords: Moringa oleifera Lam., GLUT4, glucose uptake, 3T3-L1 cells, antidiabetic.
77
Poster Presentation [PP-033]
ANTI INFLAMMATION and ANALGESICS EFFECT FROM INFUSION OF ITCHY LEAVES (Laportea decumana (Roxb.) Wedd.)
Sondang Khairani1, Lestari Rahayu1, Yeshika Krispansis Maelissa1
1Faculty of Pharmacy, Universitas Pancasila, Jagakarsa, Jakarta 12640 [email protected]
ABSTRACT
Itchy leaves have the ability as analgesics, antiinflammatory, asthma, malaria and hypertension. Empirically
in Maluku and Papua, itchy leaves are used topically to treat pain and inflammation. This study aims to
examine the antiinflammatory and analgetic effects orally of an itchy leaf decoction. Antiinflammatory testing
using the Winter method, rats were divided 5 groups negative control given aquadest, positive control given
sodium diclofenac 1,8 mg/200 g BW rats, the test group given an infusion of itchy leaves dose 0,3 g/kg BW
(I), 0,6 g/kg BW (I) and 1,2 g/kg BW (III). Analgesics testing using Siegmund method, mice were divided 5
groups, negative control given aquadest, positive control given sodium diclofenac 0,26 mg/ 20 g BW mice,
the test group given an infusion of itchy leaves dose 0,4 g/kg BW (I), 0,8 g/kg BW (II) and 1,6 g/kg BW (III).
Results of Mann-whitney test, infusion of itchy leaves had a significant anti-inflammatory and analgesic effect
on the negative control group, p value < 0,05. Precentage of positive control edema inhibition and the test
group dosage I, II, III was obtained by 27,94%; 15,97%; 25,89%; and 26,86%, percentage inhibition of positive
control and the test group dosage I, II, III was obtained by 59,42%; 43,93%; 45,70% and 80,22%. Itchy leaf
decoction has a lower antiinflammatory effect than diclofenac sodium. The analgesics effect of decoction of
itchy leaves at doses of 0,4 g/kg BW and 0,8 g/kg BW was lower, dose of 1,6 g/kg BW was higher than
diclofenac sodium.
Keywords: Anti-inflammation, analgesics, leaves itchy (Laportea decumana (Roxb.) Wedd.)
78
Poster Presentation [PP-034]
ANTI-ALOPECIA OF WASTE CACAO SKIN (Theobroma cacao L.)
Resmi Mustarichie1*, Aliya Nur Hasanah1, Dolih Gazali2
1Pharmaceutical Analysisi and Medicinal Chemistry Dept., Faculty of Pharmacy, Universitas Padjadjaran,
Indonesia 45363 2Department of Pharmaceutics, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia 45363
ABSTRACT
Hair loss is the most common hair problem. Some drugs such as Minoxidil have been shown to overcome hair
loss. However, the side effects of Minoxidil are unavoidable. Herbal treatments can be a solution to hair loss.
From the results of monitoring in the field, it was found that according to the community trust of Dinggaa
Linggajati at the foot of Mount Galungung, Tasikmalaya, the skin of cocoa fruit is usually boiled and then the
water is clamped and taped to treat the head of children who are corroded and bald. The absence of scientific
research on the truth of the properties of cocoa skin is the rationale for this research. In this study, the activity
of growth stimulant activity of cacao (Theobroma cacao L.) fruit peels. The cacao peel extract was extracted
with ethanol 96% solvent and fractionated with water, n-hexane, and ethyl acetate solvents, and then tested
for hair growth stimulant activity of extracts and waste cacao peels fraction with modified Tanaka (1980)
method. In this test the concentration of ethanol extract of cacao fruit peel was diluted to 5%, 10%, 15%, 20%
and 25% which were tested in test animals, namely rabbits. The test results showed that the ethanol extract
of cacao fruit peel effluent had a hair growth stimulating activity starting at a concentration of 15%. In testing
the fraction of cacao fruit peel fraction, it was found that the n-hexane fraction had the best activity compared
to the other fractions and positive controls (minoxidil 2%).
Keywords: Hair loss, cacao fruit peel waste, Theoborma cacao, Extraction, Fractionation.
79
Poster Presentation [PP-035]
EXAMINATION OF QUALITY PARAMETERS, TOXICITY TEST BY BSLT METHOD, ANTIOXIDANT ACTIVITY, AND INHIBITION OF α-GLUCOSIDASE ENZYME FROM
70% ETHANOL EXTRACT RAMBUTAN LEAVES (Nephelium lappaceum L.)
Greesty Finotory Swandiny1)*, Ratna Djamil2), Ammar Fajar Al-aziz3)
1)Laboratory of Biological Pharmacy, Faculty of Pharmacy, Pancasila University, Jakarta 12640 2)Laboratory of Phytochemical, Faculty of Pharmacy, Pancasila University, Jakarta 12640 3) Laboratory of Pharmacology, Faculty of Pharmacy, Pancasila University, Jakarta 12640
ABSTRACT
Rambutan plant (Nephelium lappaceum L.) is one of the plants found in Indonesia and spread in parts of
islands such as Java, Bali and Sumatera where rambutan plants easy are to grow, so the number is abundant
and various types. In this research examined the quality parameters of ethanol extract 70% of rambutan leaf
and tested toxicity, antioxidant activity, inhibiting activity of a-glucosidase enzyme from ethanol extract 70%
rambutan leaf (Nephelium lappaceum L.). Extraction of rambutan leaf and stem bark powder rambutan leaf
was conducted with maceration by using 70% ethanol as solvent, followed by extract quality parameters that
involved specific parameter and no-specific parameters, which then extracts in BSLT toxicity test, antioxidant
activity test with DPPH, and in vitro enzyme inhibiting activity test of in vitro. The results showed that ethanol
extract 70% of rambutan leaves meets standardization of extract quality parameters, and LC50 toxicity level
was 114,10 µg/ml, antioxidant activity 66,06 µg/ml, and inhibition of enzyme a-glucosidase 87,38 µg/ml.
Ethanol extract 70% of rambutan leaf meets standardization, test toxicity, has antioxidant activity and has
inhibitory activity of enzyme α-glucosidase.
Keywords: Rambutan Leaf, quality parameters, test toxicity, antioxidant activity, inhibition of enzyme α-glucosidase.
80
Poster Presentation [PP-036]
DETERMINATION OF QUALITY PARAMETERS AND ANTIOXIDANT ACTIVITY USING ABTS AND DPPH METHODS OF KELOR LEAVES (Moringa oleifera L.)
1Ratna Djamil, 2Septira Anggraeni, 3Greesty Finotory Swandiny
1Laboratory of Phyto Chemistry, Faculty of Pharmacy, Pancasila University, Jakarta 12640
2Laboratory of Pharmacology, Faculty of Pharmacy, Pancasila University, Jakarta 12640 3Laboratory of Biological Pharmacy, Faculty of Pharmacy, Pancasila University 12640
ABSTRACT
Moringa leaves are plants that are widely used as medicine. That have coumpons contains antioxidants
activity. This research aimed to got secondary metabolite data, to determine the quality parameter, and
antioxidant activity by the method of ABTS and DPPH free radical scavenging from Moringa oleifera L. leaf
extract. The results of phytochemical screening indicated that contains alkaloids, flavonoids, saponins, tannins,
steroids, and triterpenoids. The results of quality parameters indicated that the extract with consistency of a
thick, black color, with specific smell, water soluble extract content of 50.03% and ethanol soluble extract
content of 63.30%, loss on drying of 4.20%, water content of 6.54%, residual solvent 0, 48%, total ash content
of 3.60%, acid insoluble ash content of 0.51%, water soluble ash content of 2.38%, total plate count microbial
contamination (ALT) of <30 colony/gram and mold and yeast number (AKK) of < 15 colony/gram, Pb
concentration of 1.8 mg/kg, Cd concentration of 0.0658 mg/kg. Total flavonoid of 1.75%. It was concluded that
the ethanol extract 96% Moringa leaves had a smaller IC50 value are 8.75 ppm in the ABTS method compared
to the free radical reduction method using DPPH are 27.73 ppm. However, both have very strong antioxidant
activity.
Keywords: ethanol extract 96% leaf of kelor, quality test parameters, antioxidant
81
Poster Presentation [PP-037]
THE IMPACT OF CHRONIC DISEASE MANAGEMENT PROGRAM (PROLANIS) TO
IMPROVE THE CLINICAL OUTCOME OF PATIENTS WITH DIABETES MELLITUS
TYPE 2: A STUDY IN PRIMARY HEALTH CARE CENTER IN JAKARTA, INDONESIA
Yusi Anggriani1), Reise Manninda 2), Astrid Kartika Sari 3)
1,2 ,3 ) Faculty of Pharmacy Pancasila University [email protected]
ABSTRACT
Prolanis is a Chronic Disease Management Program under the Indonesian National Health Insurance for
promoting and preventing chronic disease. Main activities of Prolanis program are disease control and
counseling, scheduled education, scheduled exercise, participant’s remainder, and home visit. The aim of this
study was to evaluate the impact of prolanis on the clinical outcome in type 2 Diabetes Mellitus (DM) patients.
An observational retrospective study was evaluated for 12 months. Data was collected from medical record.
426 patients from ten primary health care center in Jakarta was selected by simple random sampling and
divided into prolanis and non-prolanis groups. The indicators were the difference of clinical outcome between
prolanis and non-prolanis patients was analyzed using Mann-Whitney test; the correlation of adherence with
clinical outcome was analyzed using Chi-Square test.The results showed that prolanis patients had a
significant improvement in Fasting Blood Glucose (FBG) compared with non-prolanis patients (P<0.05). The
adherence of prolanis patients showed a significant improvement on FBG (124 patients-58.2%). Patients with
nonadherence had lower improvement on FBG, the improvement was observed only at 16 patients (7.5%) and
60 patients (28.17%) was poor FBG. Prolanis is an effective program to improve the clinical outcome of DM
patients.
Keywords: prolanis program, clinical outcome, diabetes mellitus, primary health care center
82
Poster Presentation [PP-038]
THE PERCEPTION ABOUT TRADITIONAL MEDICINE FOR DIABETES
BY MEMBER OF PROLANIS IN BANYUMAS
Ubun Fadli Serahli1), Dyah Aryani Perwitasari1), Kintoko1), Suwijiyo Pramono2)
1)Pasca Sarjana Farmasi Universitas Ahmad Dahlan Yogyakarta 2)Fakultas Farmasi Universitas Gadjah Mada Yogyakarta
ABSTRACT
The used of traditional medicine as a diabetic therapy is increasing. Its use also influence by the characteristic
and perception of patients. The aim of this research is to determine the patients perception about traditional
medicine and to define the characteristic which can influence perception. Patients were member of PROLANIS
in Banyumas District. The cross sectional study was used as a method with purposive random sampling. The
patients perception was measured with questionaire from strong disagree to strong agree (1-5 scale). We
recruited 290 subjects. Majority of respondents was female (63,44%) and between 45–60 years old (55,86%),
high education level (38,62%), enterpreneur (27,93%) and low income (56,89%). Majority of respondents
agree with the perception of safety of traditional medicine such as gender (71,20%), Age (74,69%), education
(77,48%), Job (72,84%), income (72,73%). Age, education and income had significance relationship (p<0.05)
with the perception of acceptability of traditional medicine. No significant relationship (p>0.05) between job,
and gender with the perception.
Keyword: Diabetes, Characteristic, Perception, Traditional medicine, Prolanis
83
Poster Presentation [PP-039]
ANTI-INFLAMMATION AND ANALGESIC TEST EFFECT OF 70% ETHANOL EXTRACT OF CINNAMON BARK (Cinnamomum burmanni B1.) IN VIVO
Rika Sari Dewi, Ni Made Dwi Sandhiutami, Beatrix Melani Evangelia
Faculty of Pharmacy, Pancasila University, Jakarta, INDONESIA [email protected]
ABSTRACT
Background: Recent studies observed that cinnamon bark is known having anti-inflammation and analgesic
effect in vitro. Objective:This study aim to observe anti-inflammation and analgesic effect of cinnamon bark in
vivo. Method: Anti-inflammation test use Winter method and rat animal models. Rat is divided into five groups:
negative control that only given aquadest, positive control that given sodium diclofenac 8,2595 mg/200 g BW,
three extract groups with different doses (300 mg/kg BW, 400 mg/kg BW, 500 mg/kg BW). In analgesic test,
Siegmund method and mice animal models is used. Mice is also divided into five groups: negative control that
only given aquadest, positive control that given sodium diclofenac 0,1193 mg/20 g BW, three extract groups
with different doses (300 mg/kg BW, 400 mg/kg BW, 500 mg/kg BW). Result and Conclusion: Based on
statistical test, 70% ethanol extract of cinnamon bark have anti-inflammation and analgesic effects. The activity
from these three doses of extract have lower anti-inflammation and analgesic than sodium diclofenac. The
results also show that there is not effectiveness enchancement in increasing doses.
Keywords: Anti-inflammation, analgesic, cinnamon bark, in vivo
84
Poster Presentation [PP-040]
EFFECT OF ASPARTAME ON LIPID PROFILE AND BLOOD GLUCOSE IN WHITE RAT
Ros Sumarny, Darmono, Rika Sari Dewi, Ratna Agustina
Faculty of Pharmacy, Pancasila University, Jakarta, INDONESIA [email protected]
ABSTRACT Background: Aspartame as one of sugar-free products consumed by many people who want to maintain
calorie intake. Many recent observations indicates the negative effect of aspartame and the safety of
aspartame used still in controversy. Objective:.This study aim to observe aspartam as sugar-free products on
lipid profile and blood glucose. Method: Rats divided into 4 groups that contain six rats each group: normal
group, 126 mg/kg dose (dose I) group, 252 mg/kg dose (dose II) group, and 504 mg/kg dose (dose III) group.
Aspartam given for 90days. Blood was collected on day-0, and day-90 for glucose, total cholesterol,
triglyceride, HDL, and LDL analysis. The method used for total cholesterol, triglyceride, and LDL was
colorimetry. LDL was calculated by Friedwald’s formula. Result: Level difference between day-0 and day-90
for total cholesterol, trygliceride, LDL, HDL, and blood glucose sequentially on normal group are 10.64; 6.89;
8.58; 0.68; 9.7, dose I group are 24.23; 25.79; 24.57; 5.49; 8.2, dose II group are 29.34; 52.73; 26.21; 7.42;
6.0, and dose III group are 35.62; 57.89; 35.31; 11.27; 7.2. Conclusion: Aspartame has effect on increase of
total cholesterol, triglyceride, LDL-cholesterol level and decrease of HDL-cholesterol level. Aspartame was no
effect on blood glucose.
Keywords: Aspartame, lipid profile, blood glucose, rats
85
Poster Presentation [PP-041]
EFFECTIVENESS AND IRRITATION TEST USING COMBINATION OF JAVANESE TURMERIC EXTRACT (Curcuma xanthorrhiza Roxb) AND SOY BEAN EXTRACT
(Glycine max L. Merill)
Leni Nurlinayanti1
Ratna Djamil2
1)Development of Natural Product Cosmetic, Faculty of Pharmacy, Pancasila University
2) Laboratory of Biological Pharmacy, Faculty of Pharmacy, Pancasila University [email protected]
ABSTRACT
In this paper a study has been carried out on how to develop skin lightening cream using a combination
between of two natural ingredients, namely javanese turmeric and soybean to treat skin beauty. The results of
this study can formulate extracts of Javanese turmeric and soybean in the form of skin lightening cream and
testing their effectiveness for skin. The formulation used in this study was a combination of javanese turmeric
extract with concentrations of 1%, 3%, 5% and 10% and soybean extract with concentrations of 5% and 15%.
To test the stability of the cream quality, was accelerated for 1 month by observing the results of storage at
low temperatures (2 ° C ± 2 ° C), room temperature (30 ° C ± 2 ° C) and high temperature (40 ° C ± 2 ° C)
which includes organoleptic observation, pH and homogeneity where each formula shows physical stability for
4 weeks, pH averages 4.5 - 5.0, and no change in cream color. For testing the safety of irritation, is carried out
on rabbits with an acute dermal irritation method where the results are safe to use and do not irritate the skin.
Whereas to see the level of measurement of skin brightness carried out the effectiveness test by applying
combination cream to a number of respondents. The effectiveness of cream testing was carried out using a
Dermalab tool for several concentrations of both formulation extract combinations. The most optimal results
were combination cream with a variation of 10% javanese turmeric extract and concentration of 15% soybean
extract with L* score (skin brightness) which is higher in lightening for the skin compared to the other three
extract mixture formulas.
Keywords: Javanese turmeric extract; soybean extract; cream; skin lightening
86
Poster Presentation [PP-042]
FORMULATION OF PEEL-OFF GEL MASK FROM EXTRACT OF BISBUL LEAVES (Diospyros blancoi. A. DC) AS ANTIOXIDANT
Fahleni, Tiara Anggraini Fitri, Yuslia Noviani
Faculty of Pharmacy, Pancasila University, Jakarta [email protected]
ABSTRACT
Bisbul leaf (Diospyros blancoi A.DC) contains flavonoids, alkaloids, tannins and shows antioxidant activity with
IC50 value of 15.06 ± 0.49 µg/mL which has the potential activities to inhibited free radicals. The aims of this
study are to determine of antioxidant activity for bisbul leaf extract based on IC50 value and formulated the gel
mask which is meets the specifications both physical and chemical quality, stable and effective as an
antioxidant. The extract was made from 70% ethanol by kinetic maceration and concentrated using a rotary
evaporator. The extract was tested for antioxidant activity by DPPH method and formulated into peel-off gel
mask in various concentration of propylene glycol, they are 10, 15, and 20%. The gel mask was evaluated for
physical and chemical quality, including organoleptic, homogeneity, viscosity and rheology, spread ability,
drying time, tensile strength, pH and antioxidant activity. The stability test was carried out for two months at
40oC and also conducted evaluation of physical and chemical quality every month.
Keywords: Extract Bisbul Leaves, Antioxidant, IC50, Peel-off Gel Mask
87
Poster Presentation [PP-043]
MANAGEMENT OF LOOK ALIKE AND SOUND ALIKE (LASA) DRUGS AS HIGH ALERT MEDICINE TO REDUCE DISPENSING ERROR
Nur Miftahurrohmah, Ayu Deyana Cynthia
Faculty of Pharmacy, Universitas Pancasila, Srengseng Sawah, Jagakarsa, Jakarta Selatan, DKI Jakarta, Indonesia, 12640
ABSTRACT
Some types of drugs have similar names, shapes, and sounds (look alike sound alike / LASA). In Indonesia,
this drugs are categorized as NORUM (Nama Obat, Rupa, Ucapan Mirip). LASA drugs are included in high
alert drugs, because of the high risk of causing serious errors and unwanted drug reactions. To minimize errors
in taking LASA drugs in the dispensing process, clear management is needed regarding the storage,
placement, labelling, and inspection. This study aims to find out examples of drugs classified as LASA and
their management in hospitals. The method used is by direct observation at a hospital pharmacy installation
in Jakarta, Indonesia. The results showed that drugs including the LASA category included drugs with the
same name but different doses, same names with different dosage forms, similar dosage forms with similar
packaging. The handling of LASA drugs applied at the hospital includes the provision of LASA stickers, storage
which is interspersed with other drugs, application of tall man lettering system, and rechecking step. The
application of good LASA drug management and the compliance of pharmacy staff to do the rules stated in
management of LASA drugs are the important keys in the accuracy of therapy and patient safety.
Keywords: look alike, sound alike, LASA, NORUM, high alert.
88
Poster Presentation [PP-044]
ADDITION OF YELLOW PUMPKIN (Cucurbita moschata Durch) FLOURS AS ANTIOXIDANT IN ANALOGUE RICE BASED CASSAVA (Manihot esculenta Crantz)
AND CORN (Zea mays L.)
Diana Serlahwaty, Ryan Risandi
Faculty of Pharmacy, University of Pancasila Srengseng Sawah, Jagakarsa, Jakarta Selatan, 12640, Indonesia
ABSTRACT
The consumption of rice as main food causes less optimal use of other carbohydrate sources, such as cassava
and corn. Analog rice as a processed product is done to empower various sources of carbohydrates that are
beneficial for health. This study aimed was to make analog rice, which has antioxidant content to prevent free
radicals by adding pumpkin flour. Analog rice was made into three variations of formula; each formula contains
36.67%, 40%, 43.33% pumpkin flour. A hedonic test was conducted, and antioxidant activity was performed
by DPPH method. The results showed that the increase in the percentage of pumpkin flour affected the higher
antioxidant activity in analog rice. Formula III as the best formula contained 36.67% cassava flour, 20% corn
flour, and 43.33% pumpkin flour with antioxidant activity before and after processing each of 77.27 ppm and
98.69 ppm. The antioxidant activities of pumpkin flour and analog rice without pumpkin flour were 71.05 ppm
and 154.19 ppm. The characteristic test results as follow: the density test of Kamba 0.7396 g/mL, water
absorption 189.64%, cooking time 12 minutes, water content 7.00%, fat content 1.51% and total carbohydrate
52.60%. The addition of pumpkin flour increases the antioxidant activity of analog rice.
Keywords : Yellow Pumpkin (Cucurbita moschata Durch), analogue rice, antioxidant, DPPH.
89
Poster Presentation [PP-045]
A BRIEF REVIEW FORMULATION AND CARACTERISATION NANOSTRUCTURED LIPID CARRIER (NLC)
Garnadi Jafar 1,2, Marline Abdassah 1, Taofik Rusdiana 1
Faculty of Pharmacy Padjadjaran University, Faculty of Pharmacy Bhakti Kencana University West Java, Indonesian
ABSTRACT
Commonly, the drugs have the big particle size and the poor solubility in water. It make the expected
therapeutic effect longer and the active ingredients is hyrolized when there is water in the formulation. Lipid
nanoparticle is the drug delivery system with small particle size and using lipid in the fomulation, so it can
provide faster therapeutic effect and protect the active ingredients from degradation. Although, there is water
in the formulation, it can maintain the stability of active ingredients.
Keywords: Nanostructured Lipid Carrier, Degradation, Hydrolysis.
90
Poster Presentation [PP-046]
THE CITOTOXICITY OF Zingiber ottensii RHIZOME ON HeLa SERVICAL CANCER CELLS AND MCF-7 HUMAN BREAST CANCER CELLS
Lia Marliani1,2*, Moelyono M. W1, Eli Halimah1, Anas Subarnas1
1) Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, Sumedang, Indonesia 2) Bandung School of Pharmacy, Bandung, Indonesia
ABSTRACT
The treatment of cancer through chemotherapy gives unpleasant side effects so patients tend to choose
alternative treatment using herbal ingredients. One of the herbal ingredients that can be developed as a source
of new anti-cancer drugs is Zingiber ottensii Val., a plant of the Genus Zingiber Family Zingiberaceae.
This study determined the invitro cytotoxic activity of Zingiber ottensii Val. rhizome extract and its fraction on
HeLa servical cancer and MCF-7 human breast cancer cell lines. The extraction was done by maceration using
ethanol 70% solvent and fractionated by liquid-liquid extraction using n-hexane, ethyl acetate, butanol and
water. The cytotoxicity activity of extract and fractions was determined by MTT ((3-[4,5-dimethylthiazol-2-yl]-
2,5 diphenyl tetrazolium bromide) assay method using HeLa and MCF-7 cell lines. The MTT Assay result
showed that the ethanolic extract of Zingiber ottensii Val. rhizome was more active against MCF-7 cells (IC50
245.77 µg/ml) than HeLa cells (IC50 410.92 µg/ml) and the hexane fraction had the most active on both cancer
cells compared to other fractions. It has IC50 value against HeLa and MCF-7 respectively are 48.15 and 29.85
µg/ml. This results suggested that the n-hexane fraction has strong activity against HeLa and MCF-7 cancer
cells. The Cytotoxic active compounds from Zingiber ottensii Val. rhizome are suggested to be nonpolar
compounds. Separation of cytotoxic active compounds against HeLa cervical cancer cells and MCF-7 breast
cancer cells can be carried out in the nonpolar fraction.
Keywords: MCF-7, HeLa, Zingiberaceae, Zingiber ottensii Val., Cytotoxicity
91
Poster Presentation [PP-047]
OXIDATIVE STRESS AND PLATELET ACTIVATION IN PREHYPERTENSION SUBJECTS:
A STUDY ON F2-ISOPROSTANE, P-SELECTIN, AND CD40 LIGAND
Yati Sumiyati1, Syakib Bakri2, Mansyur Arif2, and Indriyanti Rafi Sukmawati3
1) Faculty of Pharmacy, University of Pancasila, Jakarta, Indonesia 2) Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
3) Prodia Clinical Laboratory, Jakarta, Indonesia
ABSTRACT
Oxidative stress has been implicated in the pathogenesis of hypertension, where platelet activation plays a
role in the complication of hypertension. F2-isoprostane has been identified as oxidative stress marker while
P-Selectin and CD40 ligand were related with higher platelet activation condition. The aimed of this study was
to investigate the correlation between F2-isoprostane with P-selectin and CD40L, also to determine that higher
F2-isoprostane is associated with higher P-selectin and CD40L levels in pre-hypertensive. Subjects were 51
pre-hypertensive men and women as defined by JNC 7, aged 30-60 years old with eGFR ≥45ml/minutes and
fasting glucose <126 mg/dL. Level of F2-Isoprostane, P-selectin, and CD40 ligand were measured by ELISA
method. Pearson correlation and independent t-test were used in the statistical analysis. There was no
significant correlation between F2-Isoprostane with P-selectin (p=0.374) and CD40L (p=0.552); meanwhile, a
significant correlation showed between P-selectin and CD40L (p<0.0001). Level of P-selectin and CD40L
showed significant differences between subjects with F2-isoprostane < cut off, and subjects with F2-
isoprostane ≥ cut off (p=0.002 and p=0.086). Our study showed that in prehypertension no significant
correlation between oxidative stress and platelet activation meanwhile platelet activation significantly different
in pre-hypertensive subject with low and high oxidative stress level.
Keywords: Prehypertension, platelet activation, oxidative stress
92
Poster Presentation [PP-048]
FORMULASI GEL NLC EKSTRAK KALUS DAUN MURBEI (Morus alba L.) HASIL INDUKSI MENGGUNAKAN ZAT PENGATUR TUMBUH NAA DAN BAP
Faizatun1), Erlindha Gangga, Sarah Anindita W
1)Fakultas Farmasi, Universitas Pancasila [email protected]
ABSTRAK
Daun Murbei (Morus alba L.) dengan kandungan senyawa aktif oxyresveratrol dan diketahui berfungsi sebagai
inhibitor enzim tirosinase. Tujuan penelitian ini untuk mengetahui aktivitas dari ekstrak kalus daun Murbei yang
dihasilkan dari kultur jaringan daun Murbei dengan penambahan zat pengatur tumbuh NAA dan BAP serta
formulasinya dalam gel Nanostructured lipid carrier (NLC). Pembentukan kalus daun murbei diinduksi dengan
zat pengatur tumbuh NAA 1,0 ppm dan BAP 2,5 ppm. Kalus diekstrasi menggunakan cara maserasi-sonikasi.
NLC dibuat dengan metode evaporasi pelarut dengan variasi konsentrasi campuran lipida padat-cair dan
surfaktan dalam lima formula. NLC dikarakterisasi ukuran partikel, indeks polidispersitas, zeta potensial dan
morfologi nanopartikel. NLC terbaik dibuat sediaan gel dan dievaluasi organoleptik, viskositas, sifat alir, pH,
dan uji aktivitas inhibisi enzim tirosinase pada ekstrak dan gel NLC.Lima formula NLC dikarakterisasi ukuran
partikel berturut-turut 248,0 nm, 189,8 nm, 202,9 nm, 256,3 nm, 632,8 nm, indeks polidispersitas berturut-turut
0,462, 0,578, 0,582, 0,568, dan 0,387. NLC berbentuk sferis dan nilai zeta potensial -37,37 mV. Gel berbentuk
semi padat, berwarna kuning kehijauan, homogen, viskositas 530000 cPs, sifat alir tiksotropik plastis, pH 5,26.
Aktivitas inhibisi enzim tirosinase ekstrak kalus daun Murbei memberikan hasil IC50 sebesar 75,41 µg/ml dan
pada gel NLC ekstrak kalus daun Murbei IC50 sebesar 81,11µg/ml. Disimpulkan ekstrak kalus daun Murbei
dapat digunakan sumber bahan baku sebagai pencerah kulit dengan potensi kuat dan dapat diformulasi dalam
sediaan gel Nanostructured Lipid Carriers yang memenuhi mutu fisik dan kimia.
Kata Kunci: Daun Murbei, Oxyresveratrol, Kalus, NAA & BAP, Nanostructured Lipid Carriers, Gel
93
Poster Presentation [PP-049]
ANTIOXIDANT EFFECT OF BROWN SEAWEED EXTRACT NANOPARTICLES
(Sargassum polycysticum) ON THE MODEL RATS A HIGH-FAT DIET
Sarah Zaidan1, Syamsudin2, Ratna Djamil 3, Syifa A. U4, Florentina S5, Hana A. S6
1,4,5,6Clinical and Community Pharmacy Section, Faculty of Pharmacy, Pancasila University, Jakarta, INDONESIA
2Section Pharmacology, Faculty of Pharmacy, Pancasila University, Jakarta, INDONESIA 3Phytochemical Section, Faculty of Pharmacy, Pancasila University, Jakarta, INDONESIA
ABSTRACT
Introduction: Brown seaweed has the potential as a natural antioxidant and the form of nanoparticles is
needed in order to increase the bioavailability of drugs in the systemic circulation.
Object: This study aims to obtain preparations of nanoparticles from brown grass which have antioxidant
activity.
Method: This study was conducted using a mouse model of high-fat feed diet using positive vitamin E control
at a doses of brown seaweed extract 50mg / kgBB (I), 100mg / kgBB (II), 200mg / KgBB (III). Antioxidant
parameters measured is percent inhibition of MDA levels and percent increase in catalase levels and SOD
levels.
Result: Percentage of inhibition of MDA levels doses I 50.10%, doses II 43.64%, doses III 57.90%, while the
results of percent increase in levels of catalase doses I 183.87%, doses II 69.84%, doses III 359,10% and
percent increase in levels of SOD doses I 118, 53%, doses II 94.45%, doses III 133.29%.
Conclusions: Brown seaweed extract nanoparticles (sargassum polycysticum) have antioxidant activity on
the model rats a high-fat diet.
Keyword: antioxidant, nanoparticles, sargassum
94
Poster Presentation [PP-050]
UJI AKTIVITAS ANTIHIALURONIDASE SECARA IN VITRO EKSTRAK RIMPANG LENGKUAS BELANG (Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm) DAN
PREPARASI SOLID LIPID NANOPARTICLE
Siti Umrah Noor1, Deni Rahmat1, Reyvi Denira1, Dian Ratih Laksmitawati1, Wahono Sumaryono1
1Fakultas Farmasi Universitas Pancasila, Jl. Srengseng Sawah, Jagakarsa, Jakarta 12640
ABSTRACT
Alpinia zerumbet (Pers.) BL Burtt & RM Sm contains phenolic compounds that have anti-hyaluronidase activity
which can reduce wrinkles on the skin. The purpose of this study was to produce Alpinia zerumbet extract that
has in vitro anti-hyaluronidase activity and prepare it in the form of solid lipid nanoparticles (SLN). The Alpinia
zerumbet powder was extracted with ethanol 70% and concentrated with rotary vacuum evaporator at 100
mmHg ± 50oC. The extract evaluation was performed by in vitro inhibition activity of hyaluronidase enzyme
using ELISA Reader. Preparation of SLN with Alpinia zerumbet extract was carried out using
polyethyleneglycol 8-beeswax and acrylyl glucoside by hot homogenization technique-ultrasonication method.
The characterization of the nanoparticles included particle size, polydispersity index (PDI), and potential zeta.
The results of anti-hyaluronidase (IC50) activity Alpinia zerumbet extract was 81.04 µg/mL. The nanoparticles
were meet nanoparticles requirements with particle size, PDI, and potential zeta were 138.6 nm, 0.226, and -
29,9 mV respectively. In conclusion, Alpinia zerumbet extract loaded in SLN can be potentially become a
formula for anti-hyaluronidase delivery system with the ability to reduce wrinkles.
Keywords: shell ginger extract, anti-hyaluronidase activity, Solid Lipid Nanoparticles.
95
Poster Presentation [PP-051]
MARKETING STRATEGY FOR FOOD SUPPLEMENT PRODUCTS
Nurita Andayani1, Novia Maulina2, Eufemia Citra Puri Praditya2, Sampurno2, Dian Ratih Laksmitawati1
1Undergraduate Study Program, Faculty of Pharmacy, Pancasila University, Jakarta 2Master of Pharmacy Science Study Program, Faculty of Pharmacy, Pancasila University, Jakarta
ABSTRACT
Currently vitamins, supplements, and traditional medicines are an option in an effort to maintain health.
Marketing for pharmaceutical products needs a good strategy so that pharmaceutical products can attract
more consumers. This study aimed to determine the marketing strategy by effect of electronic word of mouth
(e-WOM), brand image, above the line, and brand awareness on purchasing decisions on food supplement
products. This study used a questionnaire to obtain data. There are two products of food supplement product,
honey and immunomodulators. On honey products questionnaire questions about marketing strategies Above
the Line and Brand Awareness on purchasing decisions and use 384 respondents. On immunomodulator
products questionnaire questions about e-WOM marketing strategies and brand image on purchasing
decisions and use 384 respondents. The marketing strategy through electronic word of mouth (e-WOM) and
the strengthening of the brand image of purchasing decisions can be an alternative increasing the market
share of health supplement manufacturers. A brand that is a pioneer of a product cannot be a guarantee that
the product will have many buyers, but it is necessary to collaborate with the policy makers who recommend
the product. Marketing strategies of Honey products through Above the Line and Brand Awareness cannot
influence the purchase decision of food supplement products, but brand awareness is influenced by the
strategy of Above the Line.
Keywords: e-WOM, Brand image, Above the Line, Brand Awareness
96
Poster Presentation [PP-052]
THE PATTERN OF ANTIDIABETIC MEDICINES USE IN X HOSPITAL: A STUDY OF
OUTPATIENTS IN THE PERIOD OF JANUARY-MARCH 2018
Moordiani, Sri Wahyuni1)
1)Faculty of Pharmacy, Universitas Pancasila [email protected]
ABSTRACT
Riset Kesehatan Dasar (Riskesdas) which was held by Ministry of Health of Republic of Indonesia in 2013
showed that diabetic mellitus (DM) proportion at the age of 15 years and above almost doubled compare to
Riskesdas in 2007. Further, Indonesia was in top 10 countries for the number of people with diabetes and
undiagnosed diabetes (20-79 years) in 2017. Management of DM disease in hospitals including in outpatients
unit is important to protect patients in terms of their safety. Therefore, this study aimed to determine the use
of antidiabetic medicines quantitatively in outpatients unit of X hospital. Data was collected from sales report
of antidiabetic medicines during the period, January to March 2018. The results showed that in January 2018,
metformin 500 mg was the most antidiabetic medicine used with 53.14%, followed by gliquidone 30 mg and
acarbose 50 mg with 14.60% and 10.52%, respectively. In February 2018, top three antidiabetic medicines
sold were metformin 500 mg, acarbose 100 mg, and gliquidone 30 mg with 47.56%, 15.96%, and 13.15% of
selling, correspondingly. The similar result was identified in March 2018 selling, with metformin 500 mg,
acarbose 100 mg, and gliquidone 30 mg sold were 49.37%, 15.64%, and 12.66%, respectively. Additionally,
the results revealed that metformin remains the first choice of treatment for most patients.
Keywords: diabetes mellitus, medicines use, quantitative study
97
Poster Presentation [PP-053]
ANALYSIS OF SILICO POTENTIAL OF CHLOROGENIC ACID AS ANTI OBESITY IN RECEPTOR HUMAN FAT MASS AND OBESITY ASSOCIATED PROTEIN (FTO) IN
COMPLEX WITH 5-CARBOXY-8-HYDROXYQUINOLINE
Faridah1), Esti Mumpuni1), Wahono Sumaryono1)
1)Faculty of Pharmacy Pancasila University [email protected]
ABSTRACT
The lifestyle of modern society, consumption of fatty foods, low fiber and lack of exercise trigger the occurrence
of excess weight and obesity which can cause various diseases. This study aims to determine the interaction
between chlorogenic acid with the target protein, associated protein (FTO) 4IE4 and look for the active site of
the amino acid receptor. The target is downloaded from PDB, prepared using YASARA, preparations using
Marvin Sketch for the ligands, Internal validation of the target protein with native ligand using PLANTS
program, produced a score docking and analyzed the RMSD using YASARA program and the structure using
Marvin Sketch. Docking of test compounds and positive control compounds with target proteins using PLANTS
program. Interaction visualization from docking results using VMD. The validation result of glycerol (native
ligand) with RMSD value of 2.0678. In silico test results based on score docking of sibutramin (positive control)
and chlorogenic acid respectively were -19.993; -18,6872; -17,0098. Amino acids that bind to these three
compound, control compounds is LYS-107. Interaction between chlorogenic acid and the target protein is
slightly smaller than sibutramine and there are five amino acid residues that are bound. Chlorogenic acid
relatively active as antiobesity.
Keywords: chlorogenic acid, antiobesity, in silico.
98
Poster Presentation [PP-054]
THE IMPACT OF CHRONIC DISEASE MANAGEMENT PROGRAM (PROLANIS) TO
IMPROVE THE CLINICAL OUTCOME OF PATIENTS WITH DIABETES MELLITUS
TYPE 2: A STUDY IN PRIMARY HEALTH CARE CENTER IN JAKARTA, INDONESIA
Yusi Anggriani1), Reise Manninda 2), Astrid Kartika Sari 3)
1,2 ,3 ) Faculty of Pharmacy Pancasila University [email protected]
ABSTRACT
Prolanis is a Chronic Disease Management Program under the Indonesian National Health Insurance for
promoting and preventing chronic disease. Main activities of Prolanis program are disease control and
counseling, scheduled education, scheduled exercise, participant’s remainder, and home visit. The aim of this
study was to evaluate the impact of prolanis on the clinical outcome in type 2 Diabetes Mellitus (DM) patients.
An observational retrospective study was evaluated for 12 months. Data was collected from medical record.
426 patients from ten primary health care center in Jakarta was selected by simple random sampling and
divided into prolanis and non-prolanis groups. The indicators were the difference of clinical outcome between
prolanis and non-prolanis patients was analyzed using Mann-Whitney test; the correlation of adherence with
clinical outcome was analyzed using Chi-Square test.The results showed that prolanis patients had a
significant improvement in Fasting Blood Glucose (FBG) compared with non-prolanis patients (P<0.05). The
adherence of prolanis patients showed a significant improvement on FBG (124 patients-58.2%). Patients with
nonadherence had lower improvement on FBG, the improvement was observed only at 16 patients (7.5%) and
60 patients (28.17%) was poor FBG. Prolanis is an effective program to improve the clinical outcome of DM
patients.
Keywords: prolanis program, clinical outcome, diabetes mellitus, primary health care center
99
Poster Presentation [PP-055]
INTERACTION ANALYSIS OF ASIATIC ACID AND ITS DERIVATIVES TO THREE ISOZYME OF NITRIC ACID SYNTHASE (NOS) USING FLEXIBLE DOCKING
Ida Musfiroh*, Ilham Septiandi, Sandra Megantara, Ami Tjitraresmi, Muchtaridi
Department of Chemical Analysis and Medicinal Chemistry Faculty of Pharmacy, Universitas Padjadjaran, Bandung-Indonesia
ABSTRACT
Asiatic acid is a pentacyclic triterpenoid compound produced from Centella asiatica and it known to be anti-
inflammatory by inhibiting the pathway of the synthesis of nitric oxide synthase (NOS). This enzyme has three
isozymes: endothelial NOS (eNOS), neuronal NOS (nNOS), and inducible NOS (iNO). Excessive NO
production by the iNOS enzyme can cause inflammation, arthritis, inflammation of the intestine and cytotoxic
and mutagenic effects (conditions of oxidative stress). The main residue of the active side of the iNOS enzyme,
namely Trp372 and Glu377. Glu377 plays an important role in the process of inflammation because it forms
chain bonds with the substrate, hem, and BH4. The purpose of this study is to analysize of the asiatic acid
compound and its derivatives interaction and their conformations to active site of three NOS isozymes. The
Method was including of molecular docking of the compounds into active site of NOS enzyme with the amino
acid Glu377 to be flexible using AUTODOCK v.4.2. The results showed that AA6 compound has the best
affinity and the most potential to be more selective to active site of iNOS rather than to eNOS and nNOS
enzyme. AA6 compound has the free binding energy (ΔG) into active site of eNOS, nNOS and iNOS using
flexible docking were -3.39; 1.38; and -8.0 kcal/mol, respectively. The interaction on the active site of the eNOS
enzyme has a low affinity, as well as their interaction with Glu377 in active site of nNOS has no occured. AA6
compound has a conformation stable to interacting with the active side of the iNOS enzyme and has hydrogen
bond with amino acid residues Trp372 (bond distance of 1.95 Å). This study suggested that the compound
which is selective for the iNOS enzyme.
Keywords: Asiatic acid, iNOS, Glu377, flexible docking
100
Poster Presentation [PP-056]
ANTIMICROBIAL ACTIVITY OF EXTRACT AND FRACTIONS OF RED ALGAE (Gracilaria sp.)
Sulistiyaningsih1, Raden Bayu Indradi1, Yoppi Iskandar1, Eli Halimah2, Amelia Herlambang3, Amirah Yasmin T3, Dina Sofa I3, Ade Zuhrotun1
1Biological Pharmacy Department, 2Pharmacology and Clinical Pharmacy Department, 3Undergraduate
Pharmacy Study Program
Faculty of Pharmacy Universitas Padjadjaran
Jl. Raya Bandung Sumedang KM 21, Jatinangor 45363 1Department of Biological Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia 45363
2Faculty of Pharmacy, Universitas Padjadjaran, Indonesia 45363
ABSTRACT Red algae (Gracilaria sp.) is one of the marine biota in Indonesia which has various bioactive secondary
metabolites, making it a potential source of bioactive compounds that are very beneficial for the development
of the pharmaceutical industry such as antibacterial and antifungal. Algae from Indonesian waters are found
to contain active compounds, especially from the class of alkaloid compounds, flavonoids, and tannins. The
number of cases of infection caused by bacteria and fungus is the background of research on the potential of
red algae extracts and fractions as antibacterial and antifungus. The study was conducted through testing the
antibacterial and antifungus activity of ethanol extract and fraction and determining the Minimum Inhibition
Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the most active fraction. The method
used is agar diffusion method and determination of MIC and MBC using microdilution method. The results
showed that the antibacterial and antifungus activity of red algae (Gracilaria sp.) was only found in the ethyl
acetate fraction with the most active inhibitory zone by ethyl acetate fraction which was 15,14 mm against the
bacterium Staphylococcus aureus clinical isolate, 14,67 mm against the bacterium Pseudomonas aeruginosa
clinical isolate, 15,03 mm against the bacterium Staphylococcus epidermidis clinical isolate, and 11,08 mm
against the Candida albicans. The value of the MIC and MBC generated from the most active fraction was in
the range of 25% -50% (b/v).
Keywords: Red Algae, Antibacterial, Antifungus, MIC, MBC, Staphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus epidermidis, Candida albicans.
101
Poster Presentation [PP-057]
PATERN OF ANTIBIOTICS USAGE IN INTENSIVE CARE UNIT (ICU) OF CANCER SPECIAL HOSPITAL (CSH) IN INDONESIA
Akrom Akrom1,2,
1)Pharmacology and Clinical Department, Pharmacy Faculty, Universitas Ahmad Dahlan 2) Pusat informasi dan Kajian Obat, Universitas Ahmad Dahlan
ABSTRACT
The purpose of this study is to determine the pattern of antibiotics use using ATC/DDD and Drug Utililization
(DU) 90% in cancer patients treated in the ICUCSH from 2012-2014.
Methods: a descriptive study using ATC/DDD and DU 90%. ATC/DDD was calculated using criteria set in 2015
WHO Collaborating Center Guidelines. Cancer patients treated in the ICU CSH from 2012-2014 and receiving
antibiotics were recruited as subjects. DU90% was used as the further development of the DDD classification
system to describe drugs use in the population.
Results: 202 cancer patients were receiving antibiotic therapy with more than 50% of them were diagnosed
with pneumonia, followed by central infection (> 20%) and urinary tract infection (> 10%). The antibiotic most
frequently used was Meropenem with 33.8%. The three most frequently used antibiotics based on ATC/DDD
during the three years were Meropenem (11.84, 11.14 and 18.13 DDD/100 bed-days); Levofloxacin (3.29,
6.05 and 6,32 DDD/100 bed-days); and Ceftriaxone (3,07, 3.26 and 3.39 DDD/100 bed-days). Conclusions:
The three most frequently used antibiotics based on ATC/DDD from 2012 to 2014 were meropenem,
levofloxacin and ceftriaxone. The number of antibiotics included in DU90% declined from 2012 to 2014. The
antibiotics included in DU90% for 2014 were meropenem, levofloxacin, ceftriaxone, tazobactam/piperacillin,
ciprofloxacin and metronidazole.
Keywords: Antibiotics; ATC / DDD; DU 90%; ICU of Cancer Special Hospital; Indonesia
102
Poster Presentation [PP-058]
THE EFFECT OF INCREASING SOLUBILITY WITH COSOLVEN AND PVP ON THE PREPARATION AND CHARACTERIZATION OF BROWN SEAWEED EXTRACT
(Sargassum polycystum) NANOPARTICLE AS ANTIOXIDANTS Kartiningsih1), Deni Rahmat2), Rika Sari Dewi4) ,Anarisa Budiarti5) ,
Gita Serafika Shannon6)
Fakultas Farmasi Universitas Pancasila, Jakarta [email protected]
ABSTRACT
Brown seaweed (Sargassum polycystum) is one of the seaweed groups that has high antioxidant activity. This
activity from Fucoxanthin, a caotenoid-derivative molecules. The objective of the study is improving solubility
of brown seaweed extract in aiming to make a stable and meet the requirement of nanoparticle characterization
test. Brown seaweed was extracted then the brown seaweed extract was increased solubility using the
cosolvent and solid dispersion. The best solubility test results in each formula then be used in the process of
making nanoparticles by ionic gelation method by using chitosan as a polymer and sodium tripolyphosphate
as a crosslinker agent. Brown seaweed extract was dried by freeze drying method it became powdered brown
seaweed extract nanoparticles. From this study, it was obtained that the particle size and the potensial zeta of
cosolven-based nanoparticle and PVP-based nanoparticles were 226.2 nm and 50.40 mV ; 365.1nm, and
26.56 mV. The morphology of brown seaweed extract nanoparticles were spherical and amorphous which was
supported by TEM and XRD results. Then an antioxidant activity test was carried out by using the ABTS. IC50
values of cosolvent-based and PVP-based nanoparticles were 98.7234 bpj and 97.6313 bpj. This study
showed that statistic T paired (0.182) > 0.05 there was not significant differences of antioxidant activity between
cosolven-based nanoparticle and PVP-based nanoparticles.
Keywords: brown seaweed extract, increasing solubility, PVP, cosolvent, nanoparticles, antioxidant.
103
Poster Presentation [PP-059]
OPTIMIZATION OF SECRETED RECOMBINANT HUMAN EPIDERMAL GROWTH FACTOR PRODUCTION USING PECTATE LYASE B FROM ESCHERICHIA COLI BL21(DE3) BY CENTRAL COMPOSITE DESIGN AND ITS PRODUCTION IN HIGH
CELL DENSITY CULTURE
Sriwidodo1, Toto Subroto2, Iman P Maksum2, Tina Rostinawati3, Nasrul Wathoni1, Himmatul Ulya3,
Indah Utami Putri3
1Department of Pharmaceutic and Pharmaceutical Technology, Faculty of Pharmacy Universitas Padjadjaran
2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran 3Department of Biology Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran
Jl. Raya Bandung Sumedang Km.21 Jatinangor 45363
ABSTRACT
Human Epidermal Growth Factor (hEGF) is a potential therapeutic protein that has been widely used as a
healing agent for various chronic wounds. It induces the proliferation and metabolism of epithelial cells,
regenerate skin cells and validates skin elasticity. In the previous study, recombinant hEGF (rhEGF) had been
successfully expressed extracellularly in E. coli BL21(DE3) using Pectate lyase B (PelB) signal peptide.
Previous study has shown that the medium concentration and induction time influenced the production of
rhEGF. Thus, this study was performed to optimize both factors in flask scale by central composite design
followed by high-cell density culture to scale up rhEGF production in 2 L-working volume fermenters using the
optimized condition based on flask scale result. The present work showed that the multiplication of 1.5-fold
medium concentration with induction time 3 hours after the culture started gave the best result among another
condition in this study. Additionally, the rhEGF production in fermenter scale was identified by SDS-PAGE
Tricine and quantified by ELISA, which showed 122.40 μg of the rhEGF per milliliter medium.
Keywords: Central composite design, rhEGF, high cell-density culture, and E. coli BL21 (DE3)
104
Poster Presentation [PP-060]
Etlingera elatior FLOWER OIL ANALYSIS AND ITS PROTECTION LEVEL TEST IN MOSQUITO REPELLENT LOTION
LILIS TUSLINAH1, LUTFI ZAINI LUBIS2, LUSI NURDIANTI3
Pharmacy Study Program at STIKes Bakti Tunas Husada Tasikmalaya Cilolohan Street No. 36 Tasikmalaya, 46115
ABSTRACT
Etlingera elatior flowers contains flavonoids, terpenoids, saponins, and tannins. This study aims to determine
the content of oil compounds from Etlingera elatior flowers in n-hexan extract using the GC-MS method and
to find out their protective power towards Anopheles mosquitoes compared to the mosquito repellent lotions
on the markets. Extraction was carried out by maceration method using n-hexane solvent and it was purified
using 96% ethanol. The analysis oil was carried out by compound derivatization method from Etlingera elatior
flower’s oil through transesterification using sodium methanolic and analyzed using GC-MS. The analysis
showed that Etlingera elatior flower’s oil contained 22 types of compounds and five of them had % AUC values
between 9 - 22.16% consisting of 9,12-octadecadienoyl chloride, 9-hexadekanoat methyl ester, octadecanoate
methyl ester, 9-octadecenoic tetradecil ester, and dodecanoate methyl ester. The results of the effectiveness
test in the form of anti-mosquito lotion showed that the mosquito repellent lotion of Etlingera elatior flower F3
(7.5%) had a significant difference with the mosquito repellent lotion that had been sold on the market.
Keywords: Mosquito repellent, Etlingera elatior flower, GC-MS, lotion
105
Poster Presentation [PP-061]
SINGLE NUCLEOTIDE POLYMORPHISM OF CTLA-4 (rs5742909) IN CORRELATION WITH SCHIZOPHRENIA RISK FACTOR
Riyadi Sumirtanurdin1), James Prasetyo Laksono1), Haafizah Dania2,4), Nadira Alvi Syahrina2), Fitri Nurul Ramadhani2), Irsarina Rahma Widianingrum1), Dyah A. Perwitasari4), Rizky Abdulah2,3), Melisa
Intan Barliana1,3)
1)Department of Biological Pharmacy, Biotechnology Pharmacy Laboratory, Faculty of Pharmacy Universitas
Padjadjaran 2) Department of Pharmacology and Clinical Pharmacy, Clinical Pharmacy Laboratory, Faculty of Pharmacy
Universitas Padjadjaran 3) Center of Excellence in Higher Education for Pharmaceutical Care Innovation, Universitas Padjadjaran,
Indonesia 4) Faculty of Pharmacy, Universitas Ahmad Dahlan, Indonesia
ABSTRACT
Cytotoxic T protein lymphocyte antigen-4 (CTLA-4) plays a role in regulating the T cell system, where the
occurrence of disturbances in the system seen by imbalances in Th1 and Th2 levels is believed to be one of
the etiologies of schizophrenia. SNPs at rs5742909 in the CTLA-4 gene (CT) can affect the expression level
of CTLA-4 protein. Therefore this study aimed to determine the genotype distribution of the CTLA-4 gene
(rs5742909) in schizophrenic patients at RSJ Prof. Dr. Soerojo Magelang and identified the influence of these
genetic polymorphisms on the risk factors of schizophrenia. This research was conducted through the stage
of submitting ethical approval, primer design, DNA isolation, optimization of PCR conditions, genotyping, and
data analysis. Based on the results of the study, it is known that the genotype distribution of the CTLA-4
rs5742909 gene in schizophrenic patients was 36 CC (78.26%), 10 CT (21.73%), and no TT genotypes. On
the other hand, there was no significant relationship between genetic polymorphism of the CTLA-4 rs5742909
gene against risk factors for schizophrenia (p-value = 0.050, α = 5%)
Keywords: CTLA-4, genetic polymorphism, schizophrenia
106
Poster Presentation [PP-062]
GENOTYPE IDENTIFICATION AND EFFECT OF GENETIC POLYMORPHISM OF TPH2 rs120074175 GENE ON RISK FACTORS OF SCIZOPHRENIA
James Prasetyo Laksono1), Rizky Abdulah2,4), Dyah A. Perwitasari3), Riyadi1), Haafizah Dania2,3), Fitri Nurul Ramadhani2), Nadira Alvi Syahrina2), Irsarina Rahma Widianingrum1), Melisa Intan Barliana2,4)
1)Department Biological Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, Indonesia 2)Departement of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran,
Sumedang, Indonesia 3) Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia
4)Center of Excellence in Higher Education for Pharmaceutical Care Innovation, Universitas Padjadjaran, Indonesia
ABSTRAK The polymorphism that occurs in the TPH2 rs120074175 gene can decrease serotonin synthesis in the brain up to 80%. Decreased serotonin levels are one of the possible causes of the positive symptoms schizophrenia. This study aims to look at the genotype distribution of TPH2 rs120074175 gene on schizophrenic patients at Prof. RSJ Dr. Soerojo, Magelang and relationship between the genetic polymorphism of the TPH2 rs120074175 gene against risk factors of schizophrenia. The method used in this study was Amplification of Refractory Mutation System-Polymerase Chain Reaction (ARMS-PCR). Patients who have an A allele will show a band at 294 bp, and G allele at 240bp, with a band at 515bp acts as a control band. The results from this study showed that all respondents had a wild type GG genotype (100%) without anyone having a mutant allele A. This can conclude that the TPH2 rs120074175 gene polymorphism was not associated with risk factors for schizophrenia.
Keyword: scizophrenia, genetic polimorphism, TPH2 gene
107
Poster Presentation [PP-064]
QUALITY OF LIFE OF HYPERTENSIVE PATIENTS WITH CANDESARTAN AND CANDESARTAN-AMLODIPINE COMBINATION THERAPY AT A GOVERNMENTAL
HOSPITAL IN YOGYAKARTA, INDONESIA
Faridah Baroroh, Andriana Sari, Riviana Nur Halifah
Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta, Indonesia [email protected]
ABSTRACT
Many types of antihypertensive drugs produce different efficacies, and their use in therapy is often associated with unpleasant side effects that diminish the quality of life. This prospective cohort study intended to compare the impacts of two different treatments, namely candesartan and the combination of candesartan and amlodipine, on the quality of life of hypertensive outpatients at a government hospital in Yogyakarta, Indonesia. Starting with preliminary data survey, the analysis continued to measuring the quality of life of 100 respondents, which had met the inclusion and exclusion criteria, after three months of therapy. In the EQ5D questionnaire on quality of life, the EQ5D dimension was converted to EQ5D Index. The data analysis involved chi-square and correlation tests. The EQ-5D index ranged from 0.540 to 0.933 with an average of 0.819. Based on gender and complications, the quality of life of the patients who underwent candesartan therapy was not significantly different from those who took candesartan and amlodipine (p> 0.05). Meanwhile, based on age, occupation, income, and education, a significant difference was detected (p <0.05). The quality of life significantly correlated with age, employment, income, and education (p <0.05) but insignificantly with gender and complications (p> 0.05). As a conclusion, candesartan and candesartan-amlodipine therapies do not affect the quality of life of the patients differently, and the type of treatment is a factor that does not significantly affect the quality of life of the patients (RR; 95% CI = 1.135; 0.812-1.587).
Keywords : Quality of life, Candesartan, Candesartan-Amlodipine, Hypertension, Outpatient.
108
Poster Presentation [PP-065]
PENETAPAN KADAR FENOL DAN ANTOSIANIN TOTAL EKSTRAK ETANOL 96% BERAS MERAH (Oryza sativa L.) SECARA SPEKTROFOTOMETRI
Hasan Andriyanto, Hari Susanti
Fakultas Farmasi, Universitas Ahmad Dahlan
ABSTRAK
Beras merah mengandung fenol dan antosianin yang berfungsi sebagai antioksidan.Setiap varietas beras memiliki kadar fenol dan antosianin total yang berbeda karena kondisi lingkungan yang berbeda. Penelitian ini bertujuan untuk menetapkan kadar fenol dan antosianin total ekstrak etanol 96% pada beras merah varietas dari Kalimantan dan Jawa dengan metode Spektrofotometri UV-Vis.
Ekstrak etanol 96% beras merah (Oryza sativa L.) diperoleh dengan cara maserasi. Ekstrak etanol beras merah yang diperoleh kemudian ditentukan kadar fenol secara spektrofotometri dengan pereaksi Folin-Ciocalteu dan penentuan kadar antosianin total secara spektrofotometri dengan metode pH differential. Data kadar fenol dan antosianin total beras merah Kalimantan dan Jawa dianalisis secara statistik menggunakan Independent T-Test.
Beras merah (Oryza sativa L.) yang diekstraksi dengan etanol mengandung senyawa fenolik dan antosianin. Kadar fenol total beras merah Kalimantan sebesar 212,68 ± 2,78 mg GAE/g ekstrak, beras merah Jawa sebesar 216,43 ± 4,47 mg GAE/g ekstrak. Kadar antosianin total beras merah Kalimantan sebesar 22,13 ± 3,41 mg/100 gram, beras merah Jawa sebesar 15,86 ± 3,10 mg/100 gram.
Hasil analisis data menunjukkan kadar fenol total beras merah dari Kalimantan dan Jawa tidak terdapat perbedaan yang signifikan. Sedangkan kadar antosianin total beras merah dari Kalimantan dan Jawa terdapat perbedaan yang signifikan.
Keywords: Spektrofotometri , fenol total, antosianin total, beras merah
109
Poster Presentation [PP-066]
PENETAPAN PARAMETER MUTU DAN UJI AKTIVITAS ANTIOKSIDAN
DARI EKSTRAK RIMPANG LENGKUAS BELANG
(Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm)
Siti Umrah Noor, Faridah, Wiwi Winarti*, Monica Juwita Dewi
Fakultas Farmasi Universitas Pancasila, Jakarta 12640 [email protected]
ABSTRAK
Radikal bebas merupakan senyawa sangat reaktif yang mengandung satu atau lebih elekron yang tidak
berpasangan. Radikal bebas dapat diatasi dengan antioksidan. Salah satu tanaman yang digunakan
sebagai antioksidan adalah rimpang lengkuas belang (Alpinia zerumbet) karena memiliki kandungan
polifenol yang memiliki aktivitas antioksidan. Penelitian ini bertujuan untuk memperoleh ekstrak dengan
aktivitas antioksidan yang memenuhi persyaratan mutu ekstrak. Pada penelitian ini dibuat ekstrak etanol
70% rimpang lengkuas belang, penapisan fitokimia, pemeriksaan parameter mutu ekstrak, penetapan
kadar flavonoid total, dilanjutkan uji aktivitas penghambatan radikal bebas dengan DPPH. Hasil
penapisan fitokimia serbuk dan ekstrak etanol 70% rimpang lengkuas belang menunjukkan adanya
senyawa flavonoid, saponin, tanin katekuat, triterpenoid dan minyak atsiri. Hasil pemeriksaan parameter
mutu ekstrak menunjukkan konsistensi kental, berwarna coklat, berbau aromatis, dan rasa sepet, dengan
kadar sari larut air 16,45%, kadar sari larut etanol 10,58%, kadar abu total 5,48%, kadar abu tidak larut
asam 0,81%, kadar abu larut air 2,79%, kadar air 0,85%, susut pengeringan 18,99%, penetapan sisa
pelarut 0,84%, ALT 6,97x102 koloni/g, AKK 3,54x102 koloni/g, cemaran logam berat Pb 0,3219 mg/kg
dan Cd 0,1668 mg/kg. Hasil Uji aktivitas antioksidan ekstrak rimpang lengkuas belang diperoleh IC50
117.69 bpj dan kadar flavonoid total 1,94%. Dapat disimpulkan ekstrak rimpang lengkuas belang
mempunyai aktivitas antioksidan sedang.
Kata kunci: Lengkuas belang, parameter mutu ekstrak, antioksidan
