Alleviation of chilling injury in mangoes using
cryoprotectants
Aim
The objective of this study is to verify the feasibility of
using cryoprotectants (propylene glycol, glycerol, and sorbitol)
during the storage of mangoes under low temperatures.
Introduction
Mango is a climacteric fruit with limited shelf-life, both in
ambient conditions (25°C) and under low temperatures ( 50% of the
skin surface); 3. mild (up to 25% of the skin surface); 2. less
than mild (2-5% of skin surface), and 1. without CI symptoms(3)
Lipid peroxidation: Skin tissue (0,5 g) was ground using liquid
nitrogen and the dry powder mixed with 0.1% trichloroacetic acid
(TCA) and centrifuged (3000 x g) at 4°C for 20 minutes (Gratão et
al., 2012). The results were expressed as ηmol of MDA.g-1.MF.
Experimental design and data analysis
The experiment was set according to a completely randomized
design in a 4 (control and three cryoprotectants) x 3 (doses) x 5
(withdraws – 0, 7, 14, 21, and 28 days) factorial arrangement with
5 repetitions of one fruit. The data were subject to analysis of
variance (ANOVA) using AgroEstat and the comparisons of means of
transformed data were based on Tukey’s test at p = 0.05 level of
significance.
References
1Mitra, S.K. and Baldwin, E.A. 1997. Mango, in: Mitra S.K.(Ed),
Postharvest physiology and storage of tropical and subtropical
fruits. CAB international, New York, NY. 85-77. 2MacDonald, G. A.;
Lanier, T. C. Cryoprotectants for improving frozen-food quality.
In: Erickson M.C., Hung YC. (eds) Quality in Frozen Food. Springer,
Boston, MA, 36 p. 1997. 3Miguel, A. C. A. et al. Injúria pelo frio
na qualidade pós-colheita de mangas cv. Palmer. Revista Brasileira
de Fruticultura, 1:255-260, 2011. 4Gratão, P. L., et al.
Biochemical dissection of diageotropica and Never ripe tomato
mutants to Cd-stressful conditions. Plant Physiology and
Biochemistry, 56:79-96, 2012.
Acknowledgments
AD
abC
bB
abA
0,0
1,0
2,0
3,0
4,0
5,0
6,0
7,0
0 7 14 21 28
LP (
nM
ol M
DA
g.F
M)
Storage period (days)
ControlGlycerolPropylene glycolSorbitol
Figure 2. Skin lipid peroxidation (ηMol MDA.g-1 FW) in ‘Palmer’
mangoes treated with different cryoprotectants during cold storage
at 8.5 + 1.5℃ for 28 days.
0
1
2
3
4
0 7 14 21 28
CI s
ever
ity
(1 -
4)
Storage period (days)
Control
Glycerol
Propylene glycol
Sorbitol
Figure 1. Chilling injury severity (1 – 5) in ‘Palmer’ mangoes
treated with different cryoprotectants during cold storage at 8.5 +
1.5℃ for 28 days.
Treatment application Storage
The 7th International Horticulture Research Conference
