THE 8TH INTERNATIONAL CONFERENCE ON BIOSCIENCE AND BIOTECHNOLOGY

Biosciences and Biotechnology for a Sustainable Life

DENPASAR, 14 – 16 SEPTEMBER 2017

ORGANIZED BY FACULTY OF AGRICULTURE UDAYANA UNIVERSITY

8th ICBB 2017 | i

TIME SCHEDULE 8th INTERNATIONAL CONFERENCE ON BIOSCIENCE

AND BIOTECHNOLOGY

Venue: Post Graduate Building (3rd floor), Jl. PB. Sudirman, Denpasar – Bali

First Day: ICBB Conference (September 14th , 2017)

No Time (local time)

Activities Committee

1 08.00-09.00 Registration

2 09.00-09.40 Opening Ceremony: MC

a. Opening dance b. Speech by Chairperson of

AOBBC c. Speech by KBI (Indonesian

Biotechnology Consortium) d. Speech by Dean of Faculty of

Agriculture, Udayana University

e. Speech and official opening of ICBB by Rector of Udayana

Prof. Wirawan/ Prof Sachiko Takahi Prof. Misri Gozan Prof. Dr. Ir. I Nyoman Rai, M.S. Prof. Dr. dr. A.A. Raka Sudewi, Sp.S (K)

3 09.40-10.40

Keynote Speakers a. Prof. Takuya Marumoto b. Dr. Inez S. Loedin

Moderator

4 10.40-12.10

Invited Speakers a. Prof. Yoshihiro Natori b. Prof. Dr. Acram Taji c. Prof. Bambang Sugiarto d. Q & A

Moderator

5 12.10-13.00 Lunch Break and Pray Time

6 13.00-14.00

Invited Speakers a. Prof. I G. P. Wirawan, Ph.D b. Dr Alex Johnson c. Q & A

Moderator

7 14.00-16.30 Parallel Session a. Agriculture 1 (pest and

disease/plant protection) b. Agriculture 2 (agronomy and

plant science) c. Agriculture 3 (fertilizer/soil

science)

Moderators

8th ICBB 2017 | ii

d. Agriculture 4 (molecular biology, biochemistry, agriculture bio-engineering and health

e. Agriculture 5 (soil microbiology/soil science)

8 19.00-21.00 AOBBC meeting and dinner Alit Hotel

Second Day: ICBB Conference (September 15th , 2017)

No Time (local time)

Activities Committee

1 09.00-10.30

Invited Speakers a. Dr. Kahar Muzakkar, S.Si b. Dr. Debbie S.

Retnoningrum c. Dr. Ir. I Made Tasma, M.Sc d. Q & A

Moderator

2 10.30-17.00 KBI Meeting

3 10.30-12.00

Invited Speakers a. Prof. Young Je you b. Dr. Eng. Himawan Tri

Bayu, M.P c. Prof. Miyasaka d. Dr. Siswa Setyahadi e. Q & A

Moderator

3 12.00-13.00 Lunch Break and Pray Time

4 13.00-15.30 Parallel Session a. Agriculture 1 b. Agriculture 2 c. Agriculture 3 d. Animal science and

husbandry e. Special Lecture for

Studying in Japan (Scholarship Scheme)

Moderators

7 16.00-17.00 Closing Ceremony a. Report and thank you note

by Chairman of the Committe

b. Closing Speech by Dean of Faculty of Agriculture

MC Dr. Ir. I Dewa Putu Oka Suardi, M.Si Prof. Dr. dr. A.A. Raka Sudewi, Sp.S (K)

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AGRICULTURE 6

No Name Institution/email Title Time Moderator

1 I Nyoman Wijaya*, Wayan Adiartayasa And Made Sritamin

Agrobioecology Program Study, Faculty of Agriculture, Udayana University Phone Number 081338567146 *Email: Wijayainyoman1956@Gmail.Com

The Molecular Detection Of Citrus Vein Phloem Degeneration (Cvpd) Pathogen (Liberobacter Asiaticus) In Diaphorina Citri Kuwayama (Homoptera : Psyllidae) And Other Insects Associated With Citrus Plant

13.00-13.10 I Putu Sudiarta

2 Putu Wina Andriani Lestari*, Ida Ayu Astarini, Made Ria Defiani

Biology Master Degree Study Program, Udayana University, Bali *Email: winaandriani5@gmail.com

Virus Identification On Early Generation Seed Potatoes In Kembang Merta Village, Bali

13.10-13.20

3 Deborah Margareth1*, Ida Ayu Astarini1, Martin Joni1, I Gede Rai Maya Temaja2

1Biology Master Degree Study Program, Udayana University, Bali 2Biotechnology Master Degree Study Program, Udayana University, Bali *Email: deboramargareth48@gmail.com

Weeds and Virus Identification of Potato Fields in Pancasari Village

13.20-13.30

4 Wayan Adiartayasa, I N. Wijaya and I G. N. Bagus*

Faculty of Agriculture, Udayana University Kampus Bukit Jimbaran, Badung, Bali

The Study of the Citrus Vein Phloem Degeneration with

13.30-13.40

8th ICBB 2017 | xxxix

TABLE OF CONTENTS

TIME SCHEDULE i SCHEDULE OF PARALLEL PRESENTATION iii

Parallel Session Agriculture 1 (A1) iii Parallel Session Agriculture 2 (A2) vi Parallel Session Agriculture 3 (A3) ix Parallel Session Agriculture 4 (A4) xii Parallel Session Agriculture 5 (A5) xv Parallel Session Veterinary and Animal Husbandry 1 (V1) xviii Parallel Session Agriculture 6 (A6) xxi Parallel Session Agriculture 7 (A7) xxiv Parallel Session Agriculture 8 (A8) xxvii Poster xxx

TABLE OF CONTENTS xxxix Collaboration Project Between Udayana Univ. And Takino Filter Inc. For Environmental Restoration In Bali, Indonesia,2012~2016

52

T. Marumoto, N. Kohno and T. Simizutani Use Of In Vitro Breeding Strategies In Development Of Plants 53 Acram TAJI Production Of Virus Free And Resistance Against Mosaic Virus Using Pathogen-Derived Resistance And RNA Interference Technology In Sugarcane*

54

Bambang Sugiharto Biocatalytic Removal Of Nitrate 56 Young Je Yoo, Professor Identification Of Novel Metal Homeostasis Genes In Bread Wheat And Applications In Plant Breeding

57

Julien Bonneau, Jesse Beasley, Tonatiuh Sanchez-Palacios, Laura T. Moreno-Moyano and Alexander A. T. Johnson*

Advanced Research On Agrobacterium Tumefaciens From Crown Gall Tumor Formation To Mediate Genetic Transfer To Plant Cells*Plant Cells*

58

8th ICBB 2017 | xlv

Bali Cattle Infected With Dermatophytosis In Sobangan Village, Badung Regency Putu Ayu Sisyawati Putriningsih, and I Putu Gede Yudhi Arjentinia Detection of Problems in Renal Dogs Using Ultrasound Method

106

I Putu Gede Yudhi Arjentinia and Putu Ayu Sisyawati Putriningsih Seroprevalence Of Pig Cysticercosis In Endemic Area, Indonesia

107

Ida Bagus Ngurah Swacita, I Ketut Suada and Ida Ayu Pasti Apsari

The Index Of Bursa Fabrisius Post Vaccination Of Infectious Bursal Disease In Broiler

108

Gusti Ayu Yuniati Kencana, I Nyoman Suartha, Dewa Ayu Paranitha, Ratih Komala Sari

The Molecular Detection Of Citrus Vein Phloem Degeneration (Cvpd) Pathogen (Liberobacter Asiaticus) In Diaphorina Citri Kuwayama (Homoptera : Psyllidae) And Other Insects Associated With Citrus Plant

109

I Nyoman Wijaya, Wayan Adiartayasa And Made Sritamin Virus Identification On Early Generation Seed Potatoes In Kembang Merta Village, Bali

110

Putu Wina Andriani Lestari, Ida Ayu Astarini and Made Ria Defiani Weeds and Virus Identification of Potato Fields in Pancasari Village

111

Deborah Margareth, Ida Ayu Astarini, Martin Joni, I Gede Rai Maya Temaja

The Study Of The Citrus Vein Phloem Degeneration With Observe The Symptons Of Chlorosis And Using Primers Specific 16s Rdna On Citrus Plant In Karangasem Regency

112

Wayan Adiartayasa, I N. Wijaya and I G. N. Bagus

Plasmid Resistance Of Bradyrhizobium Japonicum From Agroforestry System To Pesticides

113

Saidatul Idiyah, Hartawati, Amir Syarifudin, Laras Pratami, Ajeng Ludvita Rani and Rahayu Hapsari

VIRUS IDENTIFICATION ON EARLY GENERATION SEED POTATOES IN KEMBANG MERTA VILLAGE, BALI

Wina Andriani, Ida Ayu Astarini, Made Ria Defiani

S2 Ilmu Biologi, Universitas Udayana

INTRODUCTION

Potato production in Indonesia continue to decrease, due to use of late generation potato tubers as seeds

Use of late generation has caused high disease infestation, particularly virus

Need to use high quality, early generation seed potatoes

Department of Agriculture, Bali and a number of farmers in Bedugul region, has started to use early generation seed potatoes.

Need to confirm the quality of seed

Research aim:

• To find out virus symptoms on early generation (G1) at Kembang Merta Village

• To confirm virus type using DAS-ELISA technique

Research Method

Symptoms observation

Horticulture Field Station, Kembang Merta Village, Tabanan, Bali

Sample were taken from G1 potato field at 3 different screen house

Fresh leaves with virus symptom were taken and keep on cool box and brought to lab

DAS – ELISA METHOD

Plate filled 100 μl antibody +

wrapped using tissue &

aluminum foil.

Overnight

Weigh each sample 4 grams & crushed

until smooth

Plate washed 3x with PBST

The scoured liquid is inserted

into the plate (100 μl)

according to the elite & incubation map ± 2 hours

Plate washed 8 x with PBST

Enter each 100 μl conjugate at each sample & incubation well

± 2 hours

Plate washed 8 x with PBST Enter PNPP (100 μl) in

each well

Incubate room temperature

30-60 minutes

ELISA reader

RESULTS 1. Virus symptoms observed

Mosaic Leaf Roll Malformasi

RESULTS 1. Virus symptoms observed

Rugose Mottle

2. Serological test (DAS-ELISA) results

Plants were virus positive: PVX, PVS, PVY and PLRV at all three potato screen house.

A

B

C

Positif PVX dan PVS

Positif PVX dan PVY

Positif PVX dan PVY

Conclusion and suggestion

Conclusion :

1. Virus symptoms observed were: mosaic, leaf roll, rugose, malformation and mottle.

2. Early generation seed potato were infected with PVX (81,81 %), PVS (27,27 %), PVY (27,27 %) and , PLRV (18,75 %).

Sugestion :

1. Improve screen house isolation and pest control should be done more intensively

• Staff employees at the Kembang Merta Village, Tabanan, Bali

Thanks You