Upload
andres
View
114
Download
0
Tags:
Embed Size (px)
DESCRIPTION
The Effectiveness of Disinfectants on Pseudomonas fluorescens. Michelle Alvarez, Kadija Bangurah & Paige Stox Department of Microbiology North Carolina State University. Inspiration. How effective are your everyday household cleaners against bacteria? - PowerPoint PPT Presentation
Citation preview
The Effectiveness of Disinfectants on
Pseudomonas fluorescens
Michelle Alvarez, Kadija Bangurah &
Paige StoxDepartment of Microbiology
North Carolina State University
Inspiration• How effective
are your everyday household cleaners against bacteria?
• Does it really kill 99% of germs?
Pseudomonas fluorescens
O Gram-Negative
O Rod- Shaped
O Multiple flagella
O Soil and marine environments
O Obligate aerobe
O The optimum growth temperature is 25-30 degrees Celsius
O Produces the antibiotic Mupirocin which treat skin, ear, and eye disorders
O Nonpathogenic and lack virulence factors
Problem/ HypothesisO Problem: Which disinfectant will
be the most effective on killing Pseudomonas fluorescens?
O Hypothesis: Clorox Bleach Gel will be the most effective on killing Pseudomonas fluorescens.
(9) Disinfectants used: 1. Vinegar- Acetic Acid
2. Hand Sanitizer- ethyl alcohol
3. Clorox Gel Bleach- Sodium Hypoclorite
4. Pine Sol- Pine Oil
5. Hand Soap- Sodium Laureth Sulfate
6. Clorox Wipes- n- Alkyl-Dimethyl Benzyl Ammonium Chlorde
7. Lysol Spray- Hydrogen Peroxde
8. Dish Detergent- Triclosan
9. Shampoo- Ammonium Laureth Sulfate
O Bioscreen C instrument
O 10x Nutrient BrothO dH20O Culture: Pseudomonas
fluorescens
Series of Dilutions: O 1: 20O 1: 30O 1: 50O 1: 100O 1: 1,000O 1: 10,000O 1: 1,000,000O Optimal Density is
measured by the bioscreen C instrument for 60 hours
Method/Materials
Preliminary Trial 1
1 14 27 40 53 66 79 92 1051181311441571701831962092222352482612740
0.5
1
1.5
2
2.5
Vinegar 1Vinegar 2Shampoo 1Shampoo 2Hand San-itizer 1Hand San-itizer 2Clorox Gel 1Clorox gel 2Pine Sol 1Pine Sol 2Hand Soap 1Hand Soap 2Clorox Wipes 1Clorox Wipes 2Lysol 1Lysol 2Dish De-tergent 1Dish De-tergent 2Control Time ( minutes)
Opt
ical
Den
sity
1:10,000 dilution
1 15 29 43 57 71 85 99 1131271411551691831972112252390
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
1:10,000 Dilution
Hand Sanitzer 1Hand Sanitzer 2Pine sol 1Pine sol 2Hand Soap 1Hand Soap 2Clorox gel 1Clorx gel 2Control 1Control 2
Time (Minutes)
Opt
ical
Den
sity
1:100,000 dilution
1 14 27 40 53 66 79 92 1051181311441571701831962092222350
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
1:100,000
Pine Sol 1Pine Sol 2Clorox gel 1Clorox gel 2Control 1Control 2
time(minutes)
Opt
ical
Den
sity
ConclusionO The top two disinfectants that
worked the best at inhibiting bacteria growth were Pine Sol and Clorox Bleach Gel.
O At 1:100,000 dilution our culture started to grow and both disinfectants were no longer capable of killing our bacteria. Therefore our hypothesis was supported!
Why?
OPine Sol: Pine Oil
OClorox Bleach Gel: Sodium Hypochlorite
Questions?