The Endomembrane System

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Cellular MembranesEndomembrane Structure % total membrane in

Hepatocyte% total in Pancreatic Exocrine Cell

Endoplasmic Reticulum (smooth)

16 <1

Endoplasmic Reticulum (rough)

35 60

Golgi 7 10

Plasma membrane 2 5

Lysosomes 0.4 N.D.

Peroxisomes 0.4 N.D.

Endosomes 0.4 N.D.

Mitochondria 39 21

Nuclear envelope (inner only)

0.2 0.7

Pulse-Label Autoradiography

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3 minutes 7 minutes

Pulse-Label Autoradiography

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37 minutes 117 minutes

The Endoplasmic Reticulum

DiOC6(3)

ER dynamics

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ER / Mitochondria

Video

“Smooth”ER

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Abnormal Pathology

sER from a chondrocyte of a patient with pseudoachondroplasia

Transition zones

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SER

RER

The rER

Supplying the NE

Movin’ on

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Note points of vesicle formation

Vesicular Traffic

donor membrane

recipient membrane

vesicle type

cell surface endosomes clathrin-coated

rER Golgi coatomerII-coated

Golgi Golgi coatomerI-coated

Golgi lysosomes clathrin-coated

Golgi cell surface coatomerI-coated

There are at least several (probably many more) distinct classes of vesicle coats/sorting mechanisms that function at different

places in the cell

coatomer-,smooth-coated vesicles

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Biochemical isolation of clathrin- coated vesicles

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Clathrin QuickTime™ and aTIFF (Uncompressed) decompressor

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Animation

Dynamin

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200 nm 100 nm

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shits wt

Breakin’ up is hard to do

Fusion

Just as there are specific mechanisms for sorting and vesicle formation, there are equally specific mechanisms for vesicle delivery

VSVGts::GFP transport

Vesicular stomatitis virus glycoprotein, temp-sensitive folding mutant

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Video

The Golgi

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Perinuclear Golgi

Biochemical stratification

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Post-Golgi transport tubules

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Video

Coatamer I Dynamics at the Golgi

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Fluorescence Recovery After Photobleaching (FRAP) of COPI::GFP

Lysosomes

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Exocytosis/Endocytosis

Peroxisomes

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