Posters (in alphabetical order of the first author) session number

poster number

ZEBET’s Activities to Promote Alternative Methods for the LD50 Potency Test in Botulinum Neurotoxin Product Testing Sarah Adler0, Barbara Grune0, Andreas Luch0, Manfred Liebsch0 0 BfR (DE-Berlin)

I 1

The future in human cell culture media Armando Aguiar0, Caio Mansano0, Guilherme Ragot0, Cesar Isaac 0, Silvana Altran0, Andre Paggiaro0, Rafael Tutihashi0, Monica Mathor1, Marcus Ferreira0 0 Faculty of Medicine Of University of Sao Paulo (BR-Sao Paulo) 1 Nuclear and Energy Research Institute (BR-Sao Paulo)

IV 85

Transepithelial electrical resistance for in-vitro toxicology Sher Ahmed0, Mélanie Favre0, Martha Liley0 0 CSEM SA (CH-Neuchatel)

V & VI 91

Dissemination of industrial research, screening and testing approaches through validation processes support tools and methodologies contributing to the 3Rs Nathalie Alepee0, Jose Cotovio0, Daniel Duche0, Gladys Ouedraogo0, Silvia Teissier0, Jean-Marc Ovigne0, Jean-Roch Meunier0 0 L'OREAL (FR-Aulnay sous Bois)

I 2

A new web-portal to support non-animal research: InVitroJobs.com Norbert Alzmann0, Christiane Baumgartl-Simons, Christiane D. Hohensee, André Schmidt 0 People for Animal Rights Germany (Menschen für Tierrechte - Bundesverband der Tierversuchsgegner e.V.) (DE-Aachen)

I 3

In vitro toxicity, mutagenicity and antimutagenicity of South African traditional medicinal plants and their preparations Roel Anthonissen0, Luc Verschaeve0, Johannes Van Staden1

V & VI 92

0 Scientific institute of public health (BE-Brussels) 1 Research Centre for Plant Growth and Development, University of KwaZulu-Natal (ZA-Pietermaritzburg) In vitro perfusion system for semi automation of long-term repeat dose toxicity testing Lydia Aschauer0, Anja Wilmes0, Martin O. Leonard1, Walter Pfaller0, Paul Jennings0 0 Division of Physiology, Department of Physiology and Medical Physics, Innsbruck Medical University (AT-Innsbruck) 1 School of Medicine and Medical Science, UCD Conway Institute, University College Dublin (IE-Dublin)

V & VI 93

Plasmacytoid dendritic cell-based assay as an in vitro alternative assay for chemical allergenicity screening Seyoum Ayehunie0, Matthew Child0, Maureen Snell0, Mitchell Klausner0, Silvia Letasiova1, Helena Kandarova2 0 MatTek Corporation (US-Ashland) 1 (SK-Bratislava) 2 MatTek IVLSL (SK-Bratislava)

III 51

Effects of pharmaceuticals and personal care products (PPCPs) on cytotoxicity, EROD activity and ROS production in a rainbow trout gonadal cell line (RTG-2) Mar Babin0, Carlos Fernández0, Rosa B. Angulo0, Mónica Gómez0, Gregoria Carbonell0 0 INIA (ES-Madrid)

II 25

Replacement strategies for animal use in the education of biotechnology technical course in Southern Brazil Róber Bachinski0, Júlio Heck1, Nádia Geisa De Souza2 0 National School of Public Health (ENSP)/Fiocruz (BR-Rio de Janeiro) 1 Federal Institute of Rio Grande do Sul (IFRS) (BR-Porto Alegre) 2 Institute of Basic Sciences for Health (ICBS)/UFRGS (BR-Porto Alegre)

I 4

What do we know about alternative methods to pesticide toxicology? A systematic review Róber Bachinski0, Enrico Saggioro0, Armando Meyer0 0 National School of Public Health (ENSP)/Fiocruz (BR-Rio de Janeiro)

I 5

Critisism to the limulus amebocyte lysate (LAL) test as a replacement method to the rabbit pyrogen test (RPT) and environmental health implications Róber Bachinski0, Enrico Saggioro0, Cristiane Caldeira1, Clarice Abreu1, Octavio Presgrave1, Nádia Geisa De Souza2, Armando Meyer0 0 National School of Public Health (ENSP)/Fiocruz (BR-Rio de Janeiro) 1 National Institute of Quality Control in Health (INCQS)/Fiocruz (BR-Rio de Janeiro) 2 Institute of Basic Sciences for Health (ICBS)/UFRGS (BR-Porto Alegre)

V & VI 94

Quantification of esterase activity in human keratinocytes and fibroblasts, ex vivo skin and reconstructed human skin models Franzisca Marie Bätz0, Günther Weindl0, Monika Schäfer-Korting0

III 52

0 Freie Universität Berlin (DE-Berlin) Use of protein and immune profiles of bacterial veterinary vaccines for batch potency testing as alternative to animal testing Elisabeth Balks0, Constanze Wolf0, Elena Alles0, Holger Lößner0, Esther Werner0 0 Paul-Ehrlich-Institut (DE-Langen)

IX 134

Establishment of an in vitro test battery for the prediction of skin sensitizing potentials of chemicals Caroline Bauch0, Eric Fabian0, Susanne Kolle0, Christina Pachel1, Tzutzuy Ramirez Hernandez0, Benjamin Wiench2, Christoph Wruck3, Bennard van Ravenzwaay0, Robert Landsiedel0 0 BASF SE (DE-Ludwigshafen) 1 Technical University Kaiserslautern (DE-Kaiserslautern) 2 Johannes Gutenberg University (DE-Mainz) 3 RWTH Aachen University (DE-Aachen)

III 53

Introduction of a new reconstructed human epidermis with integrated functional primary melanocytes Bernd Becker0, Jens Hoffmann0, Eckhard Heisler1, Sascha Hopf0, Oliver Engelking0, Horst Fuchs0 0 Cellsystems Biotechnologie Vertrieb GmbH (DE-St. Katharinen) 1 Evonik Stockhausen GmbH (DE-Krefeld)

III 54

The potential of the oocyte in vitro maturation system for the reproductive toxicology Anna Beker-van Woudenberg0, Mariska Mulderij0, André Wolterbeek0, Rob Stierum0, Cyrille Krul0 0 TNO Quality of Life (NL-Zeist)

VIII 116

Plasma Medicine- a new field of research on the biomedical applications of plasma technologies requires screening methods: the modified CAM assay and the Reconstructed Epidermis as useful models to assess the effects on angiogenesis, inflam Claudia Bender0, Axel Kramer0, Rutger Matthes0, Nils-Olaf Hübner1 0 Institute of Hygiene and Environmental Medicine (DE-Greifswald) 1 Institute of Hygiene and Environmental Medicine (DE-Greifswald)

IX 135

Resveratrol may protect proteins from toxic effects of oxidative stress in vitro Kanti Bhooshan Pandey0, Syed Ibrahim Rizvi0 0 University of Allahabad, Allahabad (IN-Allahabad)

IX 136

Phototoxic effect of TPPS4 and MgTPP4 on DNA fragmentation of HeLa cells Svatopluk Binder0, Katerina Tomankova0, Adela Daskova0, Robert Bajgar0, Hana Kolarova0 0 Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University (CZ-Olomouc)

IX 137

Persistent organic pollutants have CAG repeat and dose dependent effects on androgen receptor activity in VIII 117

vitro Christel Björk0, Hannah Nenonen0, Aleksander Giwercman1, Åke Bergman2, Lars Rylander3, Yvonne L. Giwercman0 0 Lund University (SE-Malmö) 1 Skåne University Hospital (SE-Malmö) 2 Stockholm University (SE-Stockholm) 3 Lund University (SE-Lund) The ReProTect project: Development of a novel approach in hazard and risk assessment of reproductive toxicity by a combination and application of in vitro, tissue and sensor technologies Susanne Bremer0, Marc Weimer1, Michael Schwarz2 0 Institute for Health and Consumer Protection/ Joint Research Centre (IT-Ispra) 1 2German Cancer Research Center (DE-Heidelberg) 2 Eberhard Karls University of Tübingen (DE-Tuebingen)

VIII 118

Meiotic recombination is increased in in vitro human fetal oocytes by Bisphenol A Miguel Angel Brieño0, Montserrat Garcia Caldes0 0 Universitat Autónoma de Barcelona (ES-Barcelona)

VIII 119

Transport of chlorpromazine across Caco-2 cells and measuring the free concentration by nd-SPME Jessica Broeders0, Bas Blaauboer0, Joop Hermens0 0 Institute for Risk Assessment Sciences, Utrecht University (NL-Utrecht)

II 26

Analysis of use and reliability of statistics on the use of laboratory animals in the EU Daniel Brunner1 and Helmut Appl2

1 AT-Linz 2 EUSAAT and appl communications & consulting, AT-Vienna

I 6

Specificity of the in vitro cell-based assay for marine toxins detection in natural samples

Amandine Caillaud0, Elisabeth Cañete0, Helena Eixarch0, Jorge Diogène0 0 IRTA Sant Carles de la Ràpita (ES-Sant Carles de la Ràpita)

IX 138

The BALB/c 3T3 cell transformation assay to assess the carcinogenic activity of chemicals Annamaria Colacci0, Maria Grazia Mascolo0, Stefanie Perdichizzi0, Francesca Rotondo1, Elena Morandi1, Angela Guerrini2, Antonio Gazzilli0, Paola Silingardi0, Sandro Grilli2, Monica Vaccari0 0 Environmental Protection and Health Prevention Agency Emilia Romagna Region (IT-Bologna) 1 Interdepartmental Centre for Cancer Research “G.Prodi”, University of Bologna (IT-Bologna) 2 Department of Experimental Pathology-Cancer Research Section, University of Bologna (IT-Bologna)

V & VI 95

Evolution of Pseudomonas aeruginosa virulence in infected patients revealed in a Dictyostelium host model IX 139

Pierre Cosson0 0 University of geneva (CH-Geneva) Acceptation of in vitro EpiSkin and SkinEthic RHE Skin Irritation test methods for hazard identification of chemicals Jose Cotovio0, Damien Lelievre0, Marie-Helene Grandidier0, Anne de Brugerolle1, Carine Tornier1, Jean-Roch Meunier0, Nathalie Alepee0 0 L'OREAL (FR-Aulnay sous Bois) 1 SKINETHIC (FR-Nice)

III 55

Current Status of the COLIPA Prevalidation of the Reconstructed Human Skin Micronucleus Assay (RSMN) Rodger Curren0, Marilyn Aardema1, Gladys Ouedraogo2, Kerstin Reisinger3, Stefan Pfuhler4, Monique Marrec-Fairley5 0 Institute for In Vitro Sciences, Inc. (US-Gaithersburg, MD) 1 Marilyn Aardema Consulting LLC (US-Cincinnati, OH) 2 L’Oreal Life Sciences Research (FR-Aulnay sous Bois) 3 Henkel AG & Co KGaA (DE-Duesseldorf) 4 The Procter & Gamble Co. (US-Cincinnati, OH) 5 Colipa (BE-Brussels)

III 56

Implications of postponement of the 2013 deadline for implementation of the 7th amendment to the Cosmetics Directive Alistair Currie0 0 People for the Ethical Treatment of Animals UK (GB-London)

I 7

Center for Alternatives to Animal Testing - Europe Mardas Daneshian0, Marcel Leist0, Thomas Hartung1 0 University of Konstanz (DE-Konstanz) 1 Johns Hopkins University (US-Baltimore)

I 8

Study of photodynamic effect on NIH 3T3 cell lines and bacteria Adela Daskova0, Katerina Tomankova0, Svatopluk Binder0, Robert Bajgar0, Katerina Bogdanova1, Milan Kolar1, Jiri Mosinger2, Hana Kolarova0 0 Department of Medical Biophysics, Institute of Molecular and Translational Medicine, Palacky University, Faculty of Medicine and Dentistry (CZ-Olomouc) 1 Department of Microbiology, Institute of Molecular and Translational Medicine, Palacky University, Faculty of Medicine and Dentistry (CZ-Olomouc) 2 Department of Inorganic Chemistry, Faculty of Sciences, Charles University in Prague, Prague, (CZ-Prague)

IX 140

Evaluation of the multipotent character of human skin-derived precursors Joery De Kock0, Feras Al Battah0, Tamara Vanhaecke0, Vera Rogiers0, Sarah Snykers0 0 Vrije Universiteit Brussel (VUB) (BE-Brussels)

III 57

Proposal of a chemical dataset for the development and evaluation of in vitro methods as alternatives to in vivo eye irritation testing Bart De Wever0, Nathalie Alépée1, Sandrine Bessou2, Ann De Smedt3, Penny Jones4, Béatrice Le Varlet5, Monique Marrec-Fairley6, Pauline McNamee7, Uwe Pfannenbecker8, Magalie Tailhardat9, Freddy Van Goethem3 0 Henkel (DE-Düsseldorf) 1 L'ORÉAl (FR-Aulnay Sous Bois) 2 Laboratoire Pierre Fabre (FR-Castres) 3 Johnson & Johnson Pharmaceutical Research & Development (BE-Beerse) 4 Safety and Environmental Assurance Centre Unilever (GB-Sharnbrook) 5 Links Ingénierie (FR-Paris) 6 Colipa (BE-Brussels) 7 The Procter & Gamble Company (GB-Egham, Surrey) 8 Beiersdorf (DE-Hamburg) 9 LVMH (Saint Jean de Braye Cedex)

III 58

Alternatives outreach and a new student movement for humane veterinary education in Egypt Sofia Elzaabalawy0, Mohamed Abdelbaky0, Angham Abdelhakim0, Walaa Alamir0, Mohamed Elsayed0, Mark Eryan0, Ahmed Hameda0, Nick Jukes1, Hani Mahdi0, Mina Roshdy0, Mai Shaheen0 0 Cairo University Vets for Alternatives (CUVA) (EG-Cairo) 1 InterNICHE (GB-Leicester)

I 9

A hemi-cornea model for eye irritation testing: Quality control of production and prevalidation of prediction models

Maria Engelke0, Karsten Mewes1, Heike Scholz2, Michaela Zorn-Kruppa3, Kerstin Reisinger1 0 Universität Bremen (DE-Bremen) 1 Henkel AG & Co. KGaA (DE-Düsseldorf) 2 German Animal Welfare Academy (DE-Neubiberg) 3 University Medical Center Hamburg-Eppendorf (DE-Hamburg)

III 59

Attenuateing effect of genistein on polychlorinated biphenyls induced oxidative toxicity in TM3 Leydig cell Melike Erkan0, Yasemin Tunalı-Ayun0 0 Istanbul University (TR-Istanbul)

VIII 120

A cell-free protein synthesis (translation) system based on reactor grown cells Daniel Favre0, Florian Wurm1 0 Helpatitis (CH-Lausanne) 1 Swiss Federal Institute of Technology (EPFL) (CH-Lausanne)

IX 141

Cultivation of different mammalian cell lines in a serum-free chemically defined medium Bratko Filipic0, Benjamin Gorinšek1, Lidija Gradišnik1, Avrelija Cencič1, Srečko Koren0

IV 86

0 Medical Faculty, University of Ljubljana (SI-Ljubljana) 1 Faculty of Agriculture and Life sciences, University of Maribor (SI-Maribor) Study of keratinocyte response after exposure to sensitizers in a reconstructed human epidermis Aurélie Frankart0, Alain Coquette1, Klaus-Rudolf Schroeder2, Yves Poumay0 0 University of Namur (BE-Namur) 1 SGS Life Science (BE-Wavre) 2 Henkel AG & Co. KGaA (DE-Düsseldorf)

III 60

Challenges in cytotoxicity testing of nanoparticles Eleonore Fröhlich0, Claudia Meindl0, Markus Kampel1, Thomas Pieber0 0 Medical University Graz (AT-Graz) 1 FH Joanneum (AT-Graz)

VII 110

Is a gene expression profiling useful tool for assessing the influence of ultrafine particles in in vitro tests? Katsuhide Fujita0, Masanori Horie1, Haruhisa Kato0, Shigehisa Endoh0, Ayako Nakamura0, Shinichi Kinugasa0, Yasukazu Yoshida2, Hitoshi Iwahashi2, Junko Nakanishi0 0 National Institute of Advanced Industrial Science and Technology (JP-Tsukuba) 1 National Institute of Advanced Industrial Science and Technology (JP-Osaka) 2 National Institute of Advanced Industrial Science and Technology (JP-Takamatsu)

VII 111

Androgenic properties of Halowax 1051 in ovarian follicles Ewa Gregoraszczuk0, Justyna Jerzak0, Jerzy Falandysz1 0 Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University (PL-Krakow) 1 Department of Environmental Chemistry and Ecotoxicology, University of Gdańsk (PL-Gdansk)

VIII 121

Concentration or dose: What is the proper measure for quantitative in vitro-in vivo extrapolation of toxic potency? Michael Gülden0, Hasso Seibert0 0 University Hospital Schleswig-Holstein (DE-Kiel)

V & VI 96

Cytotoxicity of beauvericin and fusaproliferin mycotoxins in Caco-2, HT-29, and Hep-G2 cells Guillermina Guillermina0, Giuseppe Mecca0, Jordi Mañes0, Maria-Jose Ruiz0 0 University of Valencia (ES-Burjassot)

V & VI 97

Effects of valproic acid (VPA) and leviteracetam (LEV) on human choriocarcinoma cell line BeWo proliferation, apoptosis and hormone secretion Patrycja Gut0, Justyna Wisniewska0, Ewa Gregoraszczuk0 0 Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University (PL-Krakow)

IX 142

The human bronchial epithelial cell line Calu-3 as in vitro model of the human airway epithelial barrier to study the transepithelial transport of insulin Tanja Hansen0, Carsten Schleh1 0 Fraunhofer Institute for Toxicology and Experimental Medicine (DE-Hannover) 1 Comprehensive Pneumology Center (CPC), Helmholtz Zentrum München, Institute of Lung Biology and Disease (iLBD) (DE-München)

II 27

A tiered approach combining the short time exposure (STE) test and the bovine corneal opacity and permeability (BCOP) assay for predicting eye irritation potential of chemicals Kazuhiko Hayashi0, Taeko Mori0, Takayuki Abo0, Kenichi Ooshima1, Takumi Hayashi1, Tomoko Komano1, Yutaka Takahashi0, Hitoshi Sakaguchi0, Hirofumi Kuwahara1, Naohiro Nishiyama0 0 Kao Corporation (JP-Tochigi) 1 Kanebo cosmetics INC. (JP-Kanagawa)

III 61

Development of a vapor cup dosing method for evaluation of chemical toxicity in the EpiAirway™ organotypic in vitro human airway model: An animal alternative model for inhalation toxicity evaluation Patrick Hayden0, Silvia Letasiova1, Kandarova Helena1, George Jackson2, Mitchell Klausner2 0 MatTek Corporation (US-Ashland, MA) 1 MatTek In Vitro Life Science Laboratories (SK-Bratislava) 2 MatTek Corporation (US-Ashland, MA)

V & VI 98

Comparison of capability of three different cell types to assess effects of chemicals on human reproductive system Tuula Heinonen0, Hanna Vuorenpää0, Ninna Toivanen0, Timo Ylikomi0 0 University of Tampere (FI-Tampere)

VIII 122

Investigations towards enhanced understanding of sensitization of chemicals: Implementation of phase I and II metabolism by coupling THP-1 activation with keratinocyte metabolism Jenny Hennen0, Michaela Kalmes0, Thomas Schettgen1, Pierre Aeby2, Carsten Goebel3, Aurelia Oberli4, Brunhilde Blömeke0 0 Universität Trier (DE-Trier) 1 Universitätsklinikum Aachen (DE-Aachen) 2 COLIPA (BE-Brussels) 3 The Procter & Gamble Co. (DE-Darmstadt) 4 Bundesamt für Gesundheit BAG (CH-Bern)

III 62

Comparative investigation of phototoxicity of chemical compounds by using the standard BALB 3T3 NRU phototoxicity test and a three-dimensional human skin model (EST 1000) Andreas Heppenheimer0 0 Harlan Cytototest Cell Research GmbH (DE-Rossdorf)

IX 143

Permeability of enrofloxacin across the in vitro Caco-2 intestinal barrier model Antoine Huguet0, Pauline Brenaut0, Jacqueline Manceau0 0 French Food Safety Agency (FR-Fougères)

II 28

Effects of microcystin LR on the integrity of the in vitro Caco-2 intestinal barrier model Antoine Huguet0, Jérôme Henri0, Aurore Besson0, Valérie Fessard0 0 French Food Safety Agency (FR-Fougères)

II 29

Towards the development of an in vitro assay to predict osteotoxicity in the developing embryo: An approach Bettina Huhse0, Dana Sittner0, Andreas Luch1, Andrea Seiler1 0 Federal Institute for Risk Assessment (DE-Berlin) 1 Institute for Risk Assessment (DE-Berlin)

VIII 123

Optimization and application with human skin explants as an in vitro alternative to animal testing for skin care Yaping Hu0, Nannan Chen0, Apostolos Pappas0, Wen-Hwa Li0, Dianne Rossetti0, Li Zhang0, Connie B. Lin0, Druie Cavender0, Miri Seiberg0 0 Johnson & Johnson (US-Skillman, NJ)

III 63

Enzyme activities for xenobiotic metabolism in human reconstructed skin models Christine Jäckh0, Veronika Blatz0, Katharina Guth0, Kerstin Reisinger1, Eric Fabian0, Ben van Ravenzwaay0, Robert Landsiedel0 0 BASF SE (DE-Ludwigshafen) 1 Henkel AG (DE-Düsseldorf)

II 30

Phototoxicity and mutagenicity hazard of pigments frequently used in cosmetics Dagmar Jirova0, Kristina Kejlova0, Hana Kolarova1, Alena Vlkova0, Katerina Tomankova1, Svatopluk Binder1, Adela Daskova1, Danuse Ocadlikova0, Hana Bendova0, David Jira2, Ilona Sperlingova0, Ludmila Dabrowska0 0 National Institute of Public Health (CZ-Praha) 1 Palacky University (CZ-Olomouc) 2 University of Veterinary and Pharmaceutical Sciences (CZ-Brno)

IX 144

Assuring safety without animal testing: Development of mechanistic understanding of dendritic cell activation response to chemical sensitisers Penelope Jones0, Andrew White 0, Samantha Fletcher0, Kristina Castle0, Seyoum Ayehunie1, Fei-Ling Lim0 0 Unilever (GB-Bedford) 1 MatTek Corporation (US-Ashland, MA)

III 64

A web-based database on main publicly available sources of toxicity data published in Russian language: contribution to REACH and 3Rs Anne Kahru0, Mariliis Sihtmäe0, Irina Blinova0

I 10

0 National Institute of Chemical Physics and Biophysics (EE-Tallinn) Variability of elimination rate and cytotoxic potency of peroxides in C6 astroglioma cell cultures Julia Kammann0, Michael Gülden0, Edmund Maser0, Hasso Seibert0 0 University Hospital Schleswig-Holstein (DE-Kiel)

II 31

Selective MeHg neurotoxicity Parvinder Kaur0, Tore Syversen0 0 NTNU, Det medisinske fakultet (NO-Trondheim)

V & VI 99

Eye irritation hazard tested in vitro in a group of chemicals and final formulations Kristina Kejlova0, Stanislav Janoušek0, Dagmar Jírová0, Hana Bendová0, Marek Malý0, Hana Kolářová1, David Jíra2 0 National Institute of Public Health (CZ-Praha) 1 Palacky University (CZ-Olomouc) 2 University of Veterinary and Pharmaceutical Sciences (CZ-Brno)

II 32

In vitro differentiated adult human liver progenitor cells display mature hepatic metabolic functions: A potential tool for in vitro pharmaco-toxicological testing

Dung Ngoc Khuu0, Sabrina Ehnert1, Omar Nyabi0, Buc-Calderon Pedro 0, Andreas Nussler1, Etienne Sokal0, Mustapha Najimi0 0 Université Catholique de Louvain (BE-Brussels) 1 Technical University Munich (DE-Munich)

II 33

Virtual reality simulators instead of animal trainings for physicians who treat acute stroke patients Monika Killer0, Michael Kral1, Stephan Oberpfalzer0, Susanne Russegger0, Gunther Ladurner0, Anton Pacher-Theinburg2 0 Neuroscience Institute/Christian Doppler Clinic (AT-Salzburg) 1 Department of Neurosurgery, University Clinic Salzburg (AT-Salzburg) 2 Veterinary authority, Land Salzburg (AT-Salzburg)

IX 145

In vitro photodynamic effect study on MCF7 cell lines Hana Kolarova0, Katerina Tomankova1, Robert Bajgar1, Svatopluk Binder1, Adela Daskova1, Kristina Kejlova2, Hana Bendova2, Dagmar Jirova2 0 Department of Medical Biophysics, Faculty of Medicine and Dentistry, Institute of Molecular and Translational Medicine, Palacky University, (CZ-Olomouc) 1 Department of Medical Biophysics, Faculty of Medicine and Dentistry, Institute of Molecular and Translational Medicine, Palacky University, (CZ-Olomouc) 2 National Institute of Public Health, (CZ-Prague)

IX 146

Testing of developmental protoxins in the embryonic stem cell test: Simulation of metabolic conversion by treatment with defined mixtures of parent compounds and their respective metabolites Johanna Kaltenhäuser0, Uschi Gruber0, Andreas Luch0, Andrea Seiler0

II 34

0 Federal Institute for Risk Assessment (DE-Berlin) Skin irritation in vitro: Evaluation of long-term reproducibility and reliability of the validated EpiDerm SIT method Helena Kandarova0, Patrick Hayden1, Mitch Klausner1, Paul Kearney1, Joseph Kubilus1, John Sheasgreen1 0 MatTek In Vitro Lie Science Laboratories, s.r.o. (SK-Bratislava) 1 MatTek Corporation (US-Ashland, MA)

III 65

Culture of organotypic, 3-dimensional tissue models – use of alternative tissue culture inserts Mitchell Klausner0, Yulia Kaluzhny0, Patrick Hayden0, Helena Kandarova1, Silvia Letasiova1 0 MatTek Corporation (US-Ashland, MA) 1 MatTek IVLSL (SK-Bratislava)

IX 147

Comparison of ³H-thymidine incorporation and non-radioactive endpoints for evaluating LLNA results during routine testing Susanne Kolle0 0 BASF SE (DE-Ludwigshafen)

III 66

Estrogen receptor transcriptional activation assays: comparing HeLa-cell- and yeast-based methods Susanne Kolle0, Hans-Albrecht Huener0, Julia Knickel0, Claudia Woitkowiak0, Andreas Verlohner0, Bennard van Ravenzwaay0, Robert Landsiedel0 0 BASF SE (DE-Ludwigshafen)

VIII 124

Modeling the free fraction of polycyclic aromatic hydrocarbons in basal cytotoxicity assays using physicochemical properties Nynke Kramer0, Ángeles Rico-Rico0, Bas Blaauboer0, Joop Hermens0 0 Utrecht University (NL-Utrecht)

V & VI 100

Development of a partition-controlled dosing system for cell assays Nynke Kramer0, Frans Busser0, Thies Oosterwijk0, Kristin Schirmer1, Beate Escher2, Joop Hermens0 0 Utrecht University (NL-Utrecht) 1 Eawag, Swiss Federal Institute of Aquatic Science and Technology (CH-Dübendorf) 2 The University of Queensland, National Research Centre for Environmental Toxicology (Entox) (AU-Brisbane)

IV 87

Alternative thinking on hazard assessment of new materials: Nanomaterials and 3Rs Cyrille Krul0, Mariska Mulderij0, Frédérique van Acker0, Mariëlle Wouters1, Susan Reinders1, Marieke op den Weegh0, Han vande Sandt0 0 TNO Quality of Life (NL-Zeist) 1 TNO Science & Industry (NL-Eindhoven)

VII 112

The Doerenkamp-Zbinden foundation’s chairs on alternatives to animal experimentation in research and education: the projects Goran Krummenacher0, Franz P. Gruber0 0 Doerenkamp-Zbinden Foundation (CH-Kuesnacht)

I 11

Communication about animal experiments and alternatives to the general public in the Netherlands Marianne Kuil0 0 Dutch Society for the Protection of Animals (NL-The Hague)

I 12

Improved cellular analysis by impedance-based real-time cell monitoring to detect compound-induced hepatic cytotoxicity Stefan Kustermann0, Markus Schmitz1, Franziska Boess0, Alexander Seiler1, Manfred Watzele1, Laura Suter-Dick0, Adrian Roth0 0 F. Hoffmann-La Roche Ltd (CH-Basel) 1 Roche Diagnostics GmbH (DE-Penzberg)

II 35

Novel biomarkers in dendritic cells contribute in understanding the skin sensitization process Nathalie Lambrechts0, Jef Hooyberghs0, Hilda Witters0, Rosette Van Den Heuvel0, Inge Nelissen0, Greet Schoeters0 0 Vito-Cardam (BE-Mol)

III 67

A novel human small intestinal functional cell model and its application in gut toxicology Tomaž Langerholc0, Lidija Gradišnik0, Avrelija Cencič0 0 University of Maribor (SI-Maribor)

II 36

Toxicity of redox-active and electrophilic organic compounds in the Tetrahymena pyriformis bioassay Anja Laqua0, Franziska Schramm1, Albrecht Paschke2, Gerrit Schüürmann1 0 Helmholtz Centre for Environmental Research - UFZ, Institute for Organic Chemistry, TU Bergakademie Freiberg (DE-Leipzig) 1 Helmholtz Centre for Environmental Research - UFZ; Institute for Organic Chemistry, TU Bergakademie Freiberg, (DE-Leipzig) 2 Helmholtz Centre for Environmental Research - UFZ (DE-Leipzig)

IX 148

Predicting clinical discomfort with the SMI assay: The effect of tonicity, pH, cooling and warming agents Joke Lenoir0, Els Adriaens0, Jean-Paul Remon0 0 Ghent University (BE-Ghent)

III 68

Clinical trial to evaluate ocular discomfort by shampoos and the relation to the Slug Mucosal Irritation (SMI) assay Joke Lenoir0, Els Adriaens0, Ilse Claerhout1, Philippe Kestelyn1, Andre Klomp2 0 Ghent University (BE-Ghent) 1 Ghent University Hospital (BE-Ghent)

III 69

2 Sara Lee Household and Body Care Research (NL-Den Haag) The EpiDerm-FT™ full-thickness in vitro human skin model: An animal alternative model for cutaneous wound healing research

Silvia Letasiova0, Patrick Hayden1, Gina Stolper1, Mitchell Klausner1 0 MatTek In Vitro Life Science Laboratories (SK-Bratislava) 1 MatTek Corporation (US-Ashland)

III 70

Comparison of the sensitivity of fibroblasts, renal epithelial cells and hepatoma cells to selected pharmaceuticals Alice Limonciel0, Anja Wilmes0, Lydia Aschauer0, Walter Pfaller0, Martin O. Leonard1, Paul Jennings0 0 Division of Physiology, Department of Physiology and Medical Physics, Innsbruck Medical University (AT-Innsbruck) 1 School of Medicine and Medical Science, UCD Conway Institute, University College Dublin, Ireland (IE-Dublin)

II 37

Cell viability and hepatocyte-typic functions in the HepaRG and LX-2 cell co-culture Mohammad Reza Lornejad-Schäfer0, Klaus Schröder0, Christine Schäfer0 0 zet + BioMed-zet Life Science GmbH (AT-Linz)

II 38

Effect of serum addition on cytochrome P450 dependent substance metabolism in primary human hepatocytes cultivated in a perfused 3D four-compartment bioreactor system Marc Lübberstedt0, Ursula Müller-Viera1, Manuela Mayer1, Fanny Knöspel0, Daniel Knobeloch2, Andreas K. Nüssler3, Jörg C. Gerlach4, Katrin Zeilinger0 0 Charité Universitätsmedizin Berlin, Berlin Brandenburg Center for Regenerative Therapies (BCRT) (DE-Berlin) 1 Pharmacelsus GmbH (DE-Saarbrücken) 2 Charité Universitätsmedizin Berlin (DE-Berlin) 3 Technische Universität München, MRI (DE-Munich) 4 McGowan Institute for Regenerative Medicine, University of Pittsburgh (US-Pittsburgh)

II 39

Assuring consumer safety without animal testing: skin allergy case study Cameron MacKay0, Jin Li0, Yeyejide Adeleye0, Michael Davies0 0 Unilever (GB-Bedford) (GB)

III 71

Wound healing model: comparison of contraction induced by cultured fibroblasts from normal skin and from hypertrophic scar Francinni Mambrini Pires Rego0, Cesar Isaac0, Silvana Cereijido Altran0, Cristiana Nicoli Mattos0, Elizabeth Mie Hosaka0, Andre Oliveira Paggiaro0, Marcus Castro Tutihashi0, Monica Mathor1, Marcus Castro Ferreira0 0 Faculty of Medicine, University of São Paulo (BR-São Paulo) 1 Nuclear and Energy Research Institute, University of São Paulo (BR-São Paulo)

III 72

CO2-euthanasia of laboratory mice is strongly criticised. Are inhalent anaesthetics a better choice? IX 149

Nicole Marquardt0, Heidrun Fink0, Jörg Luy1, Bettina Bert0 0 Institute of Pharmacology and Toxicology, Dept. of Veterinary Medicine, Freie Universität Berlin (DE-Berlin) 1 Institute of Animal Welfare and Animal Behaviour, Dept. of Veterinary Medicine, Freie Universität Berlin (DE-Berlin) How to use available alternative methods to predict skin sensitization: lessons from a benchmark exercise on preservatives Silvia Martinozzi Teissier0, Cécile Piroird0, Stéphanie Ringeissen0, Joan Eilstein0, Daniel Duche0, Jean-Marc Ovigne0, Jean-Roch Meunier0

0 L'ORÉAL (FR-Aulnay-sous-Bois)

III 73

AquaSpecTM MIR analytics as a broad and fast screening-method to classify toxic effects of agents on cells Ralf Masuch0, Franziska Kazenwadel1, Inka Pfitzner2, Dirk Dressler2, Karin Engelhart2, Andreas Wolf0 0 micro-biolytics GmbH (DE-Esslingen) 1 micro-biolytics GmbH/ Universität Stuttgart, Institut für Biochemie (DE-Esslingen/DE-Stuttgart-Vaihingen) 2 BioTeSys GmbH (DE-Esslingen)

V & VI 101

New pieces to the puzzle: Is the LLNA overestimating the sensitization potential of surfactants? Annette Mehling0, Nicholas Ball1, Stuart Cagen2, Juan-Carlos Carrillo3, Hans Certa4, Dorothea Eigler5, Roger Emter6, Frank Faulhammer7, Christine Garcia8, Cynthia Graham9, Carl Haux10, Reinhard Kreiling11, Andreas Natsch6 0 Cognis GmbH (DE-Düsseldorf) 1 Dow Europe GmbH (CH-Horgen) 2 Shell Health (US-Houston) 3 Shell International BV (NL-The Hague) 4 SASOL (DE-Marl) 5 Evonik Goldschmidt GmbH (DE-Essen) 6 Givaudan Schweiz AG (CH-Dübendorf) 7 BASF AG (DE-Ludwigshafen) 8 SEPPIC (FR-Castres) 9 Huntsman Corporation (US-The Woodlands) 10 Akzo Nobel Surface Chemistry AB (SE-Stenungsund) 11 Clariant Produkte (DE-Sulzbach)

III 74

In vitro assessment of skin and mucosae tolerance of cosmetic products Marisa Meloni0, Barbara De Servi0, Anna Ghioni0 0 VitroScreen Srl (IT-Milano)

III 75

New approach for chemical sensitizing potential assessment using THP-1 and NCTC 2544 coculture Marisa Meloni0, Barbara De Servi0, Béatrice Le Varlet1 0 VitroScreen Srl (IT-Milano) 1 Links Ingénierie (FR-Paris)

III 76

A leading case for the (dis-)approval of applications for animal experiments I 13

Claudia Mertens0, Barbara Schnüriger0 0 Zürcher Tierschutz (CH-Zürich) Nimesulide dynamics in membrane model systems: can membrane effects determine activity? Joao P. Monteiro0, André F. Martins0, Marlene Lucio1, Salette Reis1, Carlos F.G.C. Geraldes0, Teresa J. Pinheiro2, Paulo J. Oliveira0, Amália S. Jurado0 0 Center for Neuroscience and Cell Biology (PT-Coimbra) 1 REQUIMTE, Department of Physical-Chemistry, Faculty of Pharmacy, University of Porto (PT-Porto) 2 Department of Biological Sciences, University of Warwick (GB-Coventry)

IX 150

Sensitive assessment of drug-induced changes in primary human hepatocyte metabolome using a combination of GC-MS and multivariate statistics Daniel Mueller0, Saskia Mueller0, Georg Tascher0, Daniel Knobeloch1, Andreas K. Nuessler2, Elmar Heinzle0, Fozia Noor0 0 Biochemical Engineering, Saarland University (DE-Saarbruecken) 1 Department of General, Visceral and Transplantation Surgery, Charité Campus Virchow Clinic (DE-Berlin) 2 Dept. of Traumatology, TU Munich, MRI (DE-Munich)

II 40

Cultivation of primary human hepatocytes in a 3D membrane bioreactor as an alternative to 2D culture systems for improved hepatotoxicity testing Daniel Mueller0, Georg Tascher0, Ursula Mueller-Vieira1, Daniel Knobeloch2, Andreas K. Nuessler3, Marc Luebberstedt4, Katrin Zeilinger4, Elmar Heinzle0, Fozia Noor0 0 Biochemical Engineering, Saarland University (DE-Saarbruecken) 1 Pharmacelsus GmbH (DE-Saarbruecken) 2 Department of General, Visceral and Transplantation Surgery, Charité Campus Virchow Clinic (DE-Berlin) 3 Dept. of Traumatology, TU Munich, MRI (DE-Munich) 4 Charité Bioreactor Group, Campus Virchow Clinic (DE-Berlin)

II 41

The cytotoxic effects of fumonisin B1 and ochratoxin A on human and pig lymphocytes using Methyl thiazol tetrazolium (MTT) assay Mulunda Mwanza0, Melinda Kovacs1, Veronika Rajli1, Michael Francis Dutton2 0 University of Johannesburg (ZA-Johannesburg) 1 Kaposvar University (HU-Kaposvar) 2 University of Johnnesburg (ZA-Johannesburg)

V & VI 102

The proliferation of fibroblasts in human serum as alternative for fetal calf serum Cristiana Nicoli de Mattos0, Cesar Issac0, Elisabeth Hosaka0, André Oliveira Paggiaro0, Silvana Cereijido Altran0, Rafael Mamuro Carneiro Tutihashi0, Monica Mathor1, Marcus Castro Ferreira0 0 Division of Plastic Surgery, Faculty of Medicine , University of São Paulo, Cell Culture and Wound Healing Research Laboratory (BR-São Paulo) 1 Radiation Technology Center (CTR), Nuclear and Energy Research Institute, IPEN – CNEN/SP (BR-São Paulo)

IV 88

Toxicity and mode of action evaluation of commonly used adjuvants on the bases of bacterial gene expression profiles Ingrid Nobels0, Pieter Spanoghe1, Walter Steurbaut1, Ronny Blust0 0 University of Antwerp (BE-Antwerp) 1 Ghent University (BE-Ghent)

IX 151

Evaluation of a biochip technology for the prediction of metabolism-mediated toxicity Reine Note0, Marie Thomas0, Joan Eilstein0, Daniel Duché0, Gladys Ouédraogo0, Jean-Roch Meunier0 0 L'ORÉAL (FR-Aulnay sous Bois)

II 42

3D organoid like culture of Hep G2 using alginate matrix for hepatotoxicity assessment Fozia Noor0, Saskia Müller0, Daniel Müller0, Elmar Heinzle0 0 Saarland University (DE-Saarbruecken)

II 43

Chromium exposure induced cell cycle arrest in human osteoblasts Helena Oliveira0, Maria Conceição Santos0 0 University Aveiro (PT-Aveiro)

V & VI 103

Real-time and label-free monitoring of bioenergetics and cell impedance/adhesion for long and short term cell-based functional assays in vitro Stefanie Ortinau0, Sabine Drechsler0, Axel Kob0, Marcus Wego0, Ralf Ehret0 0 Bionas GmbH (DE-Rostock)

V & VI 104

Silencing of neuropathy target esterase (NTE) causes changes in gene expression during the early stages of differentiation David Pamies0, Eugenio Vilanova0, Miguel Angel Sogorb0 0 Bioengineering Institute, Migel Hernandez University (ES-Alicante)

VIII 126

Identification of antiangiogenic capability of antitumor agents based on screening for HIF-1 pathway inhibitors: characterization in comparison with in vivo tumor angiogenesis models on the examples from studies on imidazoacridinones Jolanta Paradziej-Łukowicz0, Anna Skwarska1, Grażyna Peszyńska-Sularz2, Jerzy Konopa3 0 Medical University of Gdansk (PL-Gdansk) 1 Department of Pharmaceutical Technology and Biochemistry, Gdańsk University of Technology (PL-Gdansk) 2 Medical University of Gdansk (PL-Gdansk) 3 University of Technology (PL-Gdansk)

IX 152

Characterization of cell death induced by palytoxin on an in vitro kerytinocytes cell model III 77

Marco Pelin0, Caterina Zanette0, Silvio Sosa0, Aurelia Tubaro0, Chiara Florio0 0 University of Trieste (IT-Trieste) The role of endocrine-disrupting environmental chemicals in the global epidemic of obesity Anna Pereira-Fernandes0, Caroline Vanparys0, Tine Hectors0, Ronny Blust0 0 University of Antwerp (BE-Antwerp)

IX 153

Sterile Production of platelet lysate as substitute for fetal calf serum – a concept Karin Plöderl0, Florian Hildner0, Simone Hennerbichler0, Anja Peterbauer-Scherb0, Christian Gabriel0 0 Red Cross Blood Transfusion Service of Upper Austria (AT-Linz)

IV 89

Effects of natural antioxidants vitamin C, vitamin E, quercetin and pterostilbene on beauvericin-induced cytotoxicity in CHO-K1 cells Maria-Teresa Polop0, Morgane Lavine1, Guillermina Font0, Maria-Jose Ruiz0 0 Faculty of Pharmacy. University of Valencia (ES-Valencia) 1 University of Lille (FR-Lille)

V & VI 105

Prevalidation of the ACuteTox in vitro testing strategy for the prediction of human acute oral toxicity Pilar Prieto0, Annette Kopp-Schneider1, Sven Stanzel1, Agnieszka Kinsner-Ovaskainen0 0 In-Vitro Methods Unit/ECVAM, Institute for Health and Consumer Protection, European Commission Joint Research Centre (IT-Ispra) 1 Dept. Biostatistics, German Cancer Research Centre (DE-Heidelberg)

V & VI 106

Comparison of tissue accumulation of HCB and PeCB, and action on steroidogenesis in small ovarian follicles Anna Ptak0, Jerzy Falandysz 1, Nina Konarska0, Ewa Gregoraszczuk2 0 Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University (PL-Krakow) 1 Department of Environmental Chemistry and Ecotoxicology, University of Gdańsk (PL-Gdańsk) 2 Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University (PL-Krakow)

VIII 127

Information needs for regulatory purposes – activities of the Environment Agency Austria Maria Purzner0, Simone Mühlegger0, Annemarie Losert0 0 Umweltbundesamt (AT-Vienna)

I 14

Update on recent EU activities and initiatives Kirsty Reid0 0 EWLA/Eurogroup for Animals (BE-Brussels)

I 15

Dermal xenobiotic metabolism: a comparison between human native skin, four in vitro test systems, and a liver system

II 44

Kerstin Reisinger0, H.F. Merk1, Christian Wiegand0 0 Henkel AG & Co. KGaA (DE-Duesseldorf) 1 Department of Dermatology, University Hospital, RWTH Aachen (DE-Aachen) In vitro model for the identification of respiratory sensitizing chemicals Sylvie Remy0, Sandra Verstraelen0, Jef Hooyberghs0, Greet Schoeters0 0 VITO-CARDAM (BE-Mol)

III 78

Development of an in vitro assay for the assessment of photosensitizers Hendrik Reuter0, Yeliz Tepe0, Nadine Karschuk0, Jochem Spieker0, Silke Gerlach0, Horst Wenck0, Robert Schmucker0, Klaus-Peter Wittern0, Andreas Schepky0 0 Beiersdorf AG (DE-Hamburg)

IX 154

Teratogenicity of valproic acid derivatives: Evaluation of structure-activity relationships using the embryonic stem cell test Christian Riebeling0, Klaus Becker1, Roland Buesen0, Daniel Eikel2, Johanna Kaltenhäuser0, Frauke Meyer3, Heinz Nau2, Ralph Pirow0, Brigitta Slawik0, Anke Visan0, Jutta Volland3, Horst Spielmann0, Andreas Luch0, Andrea Seiler0 0 German Federal Institute for Risk Assessment (BfR), Center for Alternative Methods to Animal Experiments ― ZEBET (DE-Berlin) 1 Bayer Schering Pharma AG, Nonclinical Drug Safety, Genetic Toxicology (DE-Berlin) 2 Department of Food Toxicology and Chemical Analysis, Food Toxicology, Center for Systemic Neuroscience Hannover, Center for Food Science, University of Veterinary Medicine Hannover (DE-Hannover) 3 Nycomed GmbH (DE-Barsbüttel)

IV 90

IUCLID 5.2 completed with in vitro data collected in a Weight of Evidence approach Costanza Rovida0 0 CAAT-EU (DE-Konstanz)

I 16

In-vitro cytotoxic potential of the aqueous extract of Securinega leucopyrus (Willd.) on human hepatoma (HepG20 cells: A preliminary study Sameera Samarakoon0, Prasanna Galhena1, Ira Thabrew0, Kamani Tennekoon0 0 Institute of Biochemistry, Molecular Biology & Biotechnology, University of Colombo (LK-Colombo) 1 Faculty of Medicine, University of Kelaniya (LK-Ragama)

I 17

Nanotechnology and animal experimentation - for better or for worse? Ursula G. Sauer0 0 Scientific Consultancy - Animal Welfare (DE-Neubiberg)

VII 113

Optimization of culture conditions for differentiation of human intestinal Caco-2 cells: serum-free medium and substrate effects

II 45

Maria Laura Scarino0, Simonetta Ferrruzza0, Carlotta Rossi0, Manuela Natoli1, Yula Sambuy0 0 National Research Institute for Food and Nutrition (INRAN) (IT-Rome) 1 Institute of neurobiology and molecular medicine (INMM) (IT-Rome)

Development of a 3D-liver cell culture model for drug metabolism and hepatotoxicity studies Christine Schäfer0, Mohammad Reza Lornejad-Schäfer0, Harald Schöffl1, Walter Pfaller2, Klaus Schröder0, Jürgen Frank3 0 zet- Centre for Alternative and Complementary Methods to Animal Testing and BioMed-zet Life Science GmbH (AT-Linz) 1 BioMed-zet Life Science GmbH (AT-Linz) 2 zet- Centre for Alternative and Complementary Methods to Animal Testing and Division of Physiology, Innsbruck Medical University (AT-Innsbruck) 3 Interfaculty Institute of Cell Biology, Department of Immunology, University of Tübingen (DE-Tübingen)

II 46

Development of an in vitro sensitization assay based on monocyte-derived dendritic cells Andreas Schepky0, Jochem Spieker0, Silke Gerlach0, Ludger Kolbe0, Walter Diembeck0, Wolfgang Pape0, Horst Wenck0, Klaus-Peter Wittern0, Hendrik Reuter0 0 Beiersdorf AG (DE-Hamburg)

III 79

Current activities of the Foundation Animalfree Research (AfR) Stefanie Schindler0 0 AnimalfreeResearch (CH-Zürich)

I 18

The Bovine Corneal Opacity and Permeability (BCOP) test for routine ocular irritation testing and its improvements within the limits of OECD test guideline 437 Arnhild Schrage0, Susanne Kolle0, Maria Rey Moreno0, Bennard van Ravenzwaay0, Robert Landsiedel0 0 BASF SE (DE-Ludwigshafen)

III 80

Toxicity of small heterocycles and their structural alerts developed by means of an in vitro test with Tetrahymena pyriformis Franziska Schramm0, Andrea Müller1, Heiko Hammer0, Albrecht Paschke0, Gerrit Schüürmann0 0 Department of Ecological Chemistry, Helmholtz Centre for Environmental Research - UFZ (DE-Leipzig) 1 Department of Proteomics, Helmholtz Centre for Environmental Research - UFZ (DE-Leipzig)

IX 155

Epigenetic modification improves hepatic differentiation of human adipose-derived mesenchymal stem cells (Ad-MSCs) for in-vitro toxicity Lilianna Schyschka0, Claudine Seeliger 0, Sabrina Ehnert0, Mihaela Culmes0, Jörg Kleef 0, Ulrich Stöckle0, Andreas K. Nussler0 0 MRI München (DE-München)

VIII 128

Epigenetic changes improve differentiation of adipose-derived mesenchymal stem cells (Ad-MSCs) to hepatocyte-like cells: possible use as an in vitro toxicity test system

VIII 129

Claudine Seeliger0, Mihaela Culmes0, Lilianna Schyschka1, Sabrina Ehnert1, Jörg Kleeff2, Ulrich Stöckle 1, Jaroslav Pelisek3, Andreas K. Nussler1 0 TU Munchen, Klinikum rechts der IsarTechnical University Munich, MRI, Dept. of Traumatology, Munich, Germany (DE-Munich) 1 Technical University Munich, MRI, Dept. of Traumatology, Munich, Germany (DE-Munich) 2 Technical University Munich, MRI, Dept. of General Surgery, Munich, Germany (DE-Munich) 3 Technical University Munich, MRI Dept. of Vascular Surgery, Munich, Germany (DE-Munich) A first approach to distinguish cytotoxic effects of diarrheic and non-diarrheic marine toxins using two human intestinal cell lines Anne-Laure Serandour0, Floriane Leurs0, Rachelle Lanceleur0, Valérie Fessard0 0 AFSSA (FR-Fougères)

IX 156

Toxicological information on REACH-relevant chemicals published in Russian language Mariliis Sihtmäe0, Irina Blinova0, Villem Aruoja0, Anne Kahru0 0 National Institute of Chemical Physics and Biophysics (EE-Tallinn)

I 19

Toxicological endpoints to assess developmental bone toxicity in vitro Dana Sittner0, Bettina Huhse0, Ariane Kretlow0, Andreas Luch0, Andrea Seiler0 0 Federal Institute for Risk Assessment (DE-Berlin)

VIII 130

Improving the sensitivity of organotypic in vitro assays for eye irritation testing: The Ex Vivo Eye Irritation Test (EVEIT) Felix Spöler0, Markus Frentz1, Norbert F. Schrage1 0 Institute of Semiconductor Electronics, RWTH Aachen University (DE-Aachen) 1 Aachen Centre of Technology Transfer in Ophthalmology (ACTO) (DE-Aachen)

III 81

The AltTox website – devoted to non-animal methods for toxicity testing Horst Spielmann0, Martin Stephens1, Grace Patlewicz2 and Hajime Kojima3 0 FU Berlin (DE-Berlin) 1 HSUS (US-Washington DC) 2 DuPont Haskell (US-Newark) 3 JaCVAM (JP-Tokyo)

I 19 A

Planned future developments of lung model for usage in inhalative substance testing Katharina Stiglbrunner0, Markus Wurm0, Peter Krösl0, Andreas Drauschke0 0 FH Technikum Wien (AT-Vienna)

V & VI 107

Introducing a new scientific society for cellular and computational toxicology in North America: ASCCT Kristie Sullivan0, Erin Hill1, Rodger Curren1

I 20

0 Physicians Committee for Responsible Medicine (US-Oakland) 1 Institute for In Vitro Sciences (US-Gaithersburg) Development of a human corneal epithelium model utilizing a collagen vitrigel membrane and the changes of its barrier function induced by exposing eye-irritant chemicals Toshiaki Takezawa0, Kazunori Nishikawa1, Pi-Chao Wang1 0 National Institute of Agrobiological Sciences (JP-Tsukuba) 1 University of Tsukuba (JP-Tsukuba)

III 82

Quality QSAR as an alternative assessment tool Tarmo Tamm0, Mehis Pilv0, Jane Paju0, Mati Karelson0 0 Molcode Ltd. (EE-Tartu)

V & VI 108

Secretome analysis of primary human hepatocytes in 2D- and 3D-culture for in-vitro toxicology Georg Tascher0, Daniel Müller0, Daniel Knobeloch1, Andreas Nüssler2, Katrin Zeilinger3, Elmar Heinzle0, Fozia Noor0 0 Biochemical Engineering, Saarland University (DE-Saarbrücken) 1 Department of General, Visceral and Transplantation Surgery, Charité Campus Virchow Clinic (DE-Berlin) 2 Department of Traumatology, MRI, TU Munich (DE-Munich) 3 Charité Bioreactor Group, Campus Virchow Clinic (DE-Berlin)

II 47

Animal testing issues during REACH Implementation Katy Taylor0 0 BUAV (GB-London)

I 21

Pre-validation of a two-tiered approach to determine the skin sensitizing capacity and potency of chemicals Marc Teunis0, Johanna Louter0, Saertje Verkoeijen0, Judith Reinders1, Emanuella Corsini2, Sue Gibbs1, Raymond Pieters0, Erwin Roggen3 0 Utrecht University of Applied Scientic Research (NL-Utrecht) 1 VU University Medical Centre (NL-Amsterdam) 2 University Milan (IT-Milan) 3 Novozymes (DK-Bagsvaerd)

III 83

In vitro cytotoxicity and phototoxicity study of the cosmetics pigments Katerina Tomankova0, Hana Kolarova0, Svatopluk Binder0, Adela Daskova0, Kristina Kejlova1, Hana Bendova1, Dagmar Jirova1 0 Department of Medical Biophysics, Institute of Molecular and Translational Medicine, Faculty of Medicine, Palacky University (CZ-Olomouc) 1 National Institute of Public Health (CZ-Prague)

IX 157

Animal experimentation and scientific knowledge: a Fleckian thought style? Thales Trez0 0 Universidade Federal de Alfenas (BR-Florianópolis)

I 22

Development of in vitro skin sensitization test using three-dimensional human skin model consisting of dendritic cells, keratinocytes and fibroblasts on collagen vitrigel membrane Tadashi Uchino0, Toshiaki Takezawa1, Yoshiaki Ikarashi 0, Tesuji Nishimura0 0 National Institute of Health Sciences (JP-Tokyo) 1 National Institute of Agrobiological Sciences (JP-Tsukuba)

III 84

In vitro prediction of teratogenic effects using pathway-specific reporters: the ReProGlo assay Frederik Uibel0, Michael Schwarz0 0 Eberhard Karls University Tübingen (DE-Tübingen)

VIII 131

The in vitro BALB/c 3T3 cell transformation assay to screen the anti-transforming activity of chemical compounds Monica Vaccari0, Maria Grazia Mascolo0, Francesca Rotondo1, Paola Silingardi0, Elena Morandi1, Stefania Perdichizzi0, Angela Guerrini2, Sandro Grilli 2, Annamaria Colacci0 0 Environmental Protection and Health Prevention Agency ARPA Emilia-Romagna (IT-Bologna) 1 Interdepartmental Centre for Cancer Research “G.Prodi”, University of Bologna (IT-Bologna) 2 Department of Experimental Pathology-Cancer Research Section, University of Bologna (IT-Bologna)

IX 158

Towards a mechanism-based safety test for acellular pertussis vaccines S.F.C. Vaessen0, M.W.P. Bruysters1, R.J. Vandebriel1, S. verkoeijen0, C.A. Krul0, A.M. Akkermans1 0 University of Applied Sciences Utrecht (NL-Utrecht) 1 National Institute for Public Health and the Environment (NL-Bilthoven)

IX 159

Reporting animal research in journals: contribution to the 3Rs Janet van der Bent0, Marjolein van Boxel1, Jan van der Valk0 0 Dept. Animals in Science and Society, Fac. Veterinary Medicine, Utrecht University (NL-Utrecht) 1 Netherlands Knowledge Centre on Alternatives to Animal Use (NL-Utrecht)

I 23

Toxicogenomics in an estrogenic screening context: added value to classical single endpoint assays? Caroline Vanparys0, Tine Hectors0, Ingrid Nobels0, Anna Pereira-Fernandes0, Ronny Blust0, Wim De Coen1 0 University of Antwerp (BE-Antwerp) 1 ECHA (FI-Helsinki)

VIII 132

PreadyPortTM, a novel ready-to-use system for in vitro drug transporter evaluation Maya Vilà0, Marton Jani1, Elisabet Marti0, Angela Toledo0 0 Advancell (ES-Barcelona) 1 Solvo Biotechnology (HU-Budapest)

II 48

ISOCYP-TOX: A new ready-to-use concept for in vitro evaluation of biotransformation-mediated toxicity II 49

Maya Vilà0, Josep-Maria Miquel0, Guillermo Quintas1, Myriam Fabre2 0 Advancell (ES-Barcelona) 1 Advancell (ES-Valencia) 2 (ES-Barcelona) Connexin32 hemichannels facilitate the apoptotic-to-necrotic transition during Fas-mediated hepatocyte cell death Mathieu Vinken0, Elke Decrock1, Elke de Vuyst1, Marijke de Bock1, Roosmarijn Vandenbroucke1, Bruno de Geest1, Joseph Demeester1, Niek Sanders1, Tamara Vanhaecke0, Luc Leybaert1, Vera Rogiers0 0 Vrije Universiteit Brussel (BE-Brussels) 1 Universiteit Gent (BE-Ghent)

II 50

Risk assessment for nanotoxicology: animal welfare aspects Kristina Wagner0 0 Deutscher Tierschutzbund -Akademie für Tierschutz (DE-Neubiberg)

VII 114

Multi-walled carbon nanotubes - toxicological effects on human cells Elisabeth Weber0, Letizia Farmer 0, Christine Höpfner0, Nina Kager0, Andreas Krenauer0, Alexander Graff1, Klaus Mauthner2, Eva Valic3 0 Seibersdorf Labor GmbH (AT-Seibersdorf) 1 Österreichische Staub- und Silikosebekämpfungsstelle (AT-Leoben) 2 C-Polymers GmbH (AT-Klosterneuburg) 3 Austrian Woker’s Compensation Board (AUVA) (AT-Vienna)

VII 115

Evaluation of anti-inflammatory and atrophogenic effects of glucocorticoids on reconstructed human skin models Günther Weindl0, Francesca Castello0, Monika Schäfer-Korting0 0 Freie Universität Berlin, Institut für Pharmazie (Pharmakologie und Toxikologie) (DE-Berlin)

IX 160

IMOLA-IVD: A cytosensor microphysiometer of the 2nd generation Joachim Wiest0 0 cellasys GmbH (DE-Munich)

IX 161

Fulfilling data requirements for the U.S. EPA endocrine disruptor screening program using existing and non-traditional data Catherine Willett0, Nancy Beck1, Jeffery Brown0, Joseph Manuppello0, Samantha Suiter0, Kristie Sullivan1 0 People for the Ethical Treatment of Animals (US-Norfolk, VA) 1 Physicians Committee for Responsible Medicine (US-Washington, DC)

VIII 133

A test system with high sensitivity for developmental neurotoxicants based on differentiation of murine VIII 133 A

embryonic stem cells to neurons Bastian Zimmer0, Philipp Kügler0, Suzanne Kadereit0 and Marcel Leist0 0 Doerenkamp-Zbinden Chair, University of Konstanz (DE-Konstanz) Protective effect of curcumin and its soluble form on Benzoquinone treated human erythrocytes in vitro Fatemeh Zolghadr0, Masih Sherafatian1, Majid Sadeghizadeh2 0 Genetics Department, Biological Sciences Faculty,Tarbiat Modares University (IR-Tehran) 1 Biology Department, Tehran University (IR-Tehran) 2 Genetics Department, Biological Sciences Faculty,Tarbiat Modares University (IR-Tehran)

V & VI 109

Corporate social responsibility and laboratory animals Marja Zuidgeest0 0 Proefdiervrij (NL-The Hague)

I 24

Poster session I Thursday, September 2nd 2010 17:00-19:30 session numbers I to IV survey of the posters with presence of the authors

Poster session II Friday, September 3rd 2010 16:00-18:00 session numbers V to IX survey of the posters with presence of the authors