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The New Strategy To Reverse Aging by Dr Estella Loing
Citation preview
Newstrategytoreverse
signsofaging
SWSCC Educational Seminar & Suppliers Day
August 23, 2012 - Las Colinas
Who is Unipex? 2
questforeternallifeimmortality
alchemistshuntedinvainfortherejuvenatingPhilosophersstone
SkinAging
Chronologicalaging
Photoaging
External/Internalfactors:pollution,foods,alcohol,tobacco,emotions
Genetic
Antiagingcream
Plasticsurgery
Makeupandphotoshop
Strategiestoreverseskinaging SkinAging
Physiologicalchangeswithage
Hyper orhypopigmentationSeborrheic keratoses
Dry,scalyskinIncreaseinskinfragility(barrierfunction)
Increaseinroughness,wrinklesLaxity
EasybruisingDecreaseimmunefunction
AGING
MatureskinYoungskin
Stratumcorneum CompactFlaky
Dermis
Epidermis
Hypodermis
SC
DEJ
EpidermisThickness =properskinbarrier
Keratinocytes,Mc: proliferation,maturationProteoglycanssynthesis
Thick Thin
++++++
DermislevelECMcomponent(collagens,elastin..)
MMPsactivitiesImmunefunctions
Low+++
+++
FlatDermal Epidermal junctionDermis/epidermiscohesion
Wavy+++
HypodermislevelSubcutaneousfat+++
Skinchangeswithage
AGING
Ourstrategytoreverseskinaging
DermislevelECMcomponent(collagens,elastin..)
MMPsactivitiesImmunefunctions
Low
Stratumcorneum CompactFlakyEpidermis
Thickness =properskinbarrierKeratinocytes,Mc,proliferation,maturation
Proteoglycanssynthesis
Thick Thin
++++++
FlatDermal Epidermal junctionDermis/epidermiscohesion
Wavy
Dermis
Epidermis
Hypodermis
SC
DEJ
HypodermislevelSubcutaneousfat
+++
+++
+++
+++ +++ Progerin+/- Progerin
AGING
Structuralagingduetosenescencemarker:progerin
Lamin A/CFrom Cao et al. 2011
Thisprocessofaginggallopstoaboutseventimesthenormalrate
Progerin asanewmarkerofaging Progerin isanalteredformoftheLamin Aprotein(nuclearintermediatefilamentprotein) Accumulationofprogerin innucleus OriginallyassociatedwiththeHutchinsonGilfordSyndrome(=Progeria)
Progerin andaccelerationofaging Accumulation ofprogerin inducesvariousdefectsinnuclearstructureandfunctions Alterationscellcycleprogressioncausedbyprogerin knowntoacceleratehumanaging
1
Progerin is expressed in healthy cells (fibroblasts and terminally differentiated keratinocytes mainly) andaccumulated with age (3% per year)
The same molecular mechanisms responsible for premature aging in progeria act at low level in healthy cells
Cell nuclei from old individuals acquire defects similar to those of progeria patient cells
Progerin inhealthycells
Progerin hasbeenlinkedtophysiologicalageingaspresenceofprogerin isconcomitantwithalterationsofthedermalcompartment suchasdisorganizedconnectivetissue
From Scaffidi et al. 2008
1
9yearsold 96yearsold Progeria
TT2 abiomimeticstructuralpeptideFocus on biomimetic peptide derived from structural proteins and enzymes implicated in the maintenance ofdermal structure and connective tissue organizationTT2 (INCI: F3Acetyl Tripeptide2) : 3 amino acid peptide derived biomimetic of Elafin, an enzyme inhibitor
1
Protocol Maturehumandermisfibroblastswereincubatedfor96hourswithTT2peptide TheprogerinwasquantifiedusinganELISAmethodology.Resultsaregiveninpgofprogerinpergofproteins
TT2peptide decreasessignificantlytheproductionofprogerin,attestingitsefficacyonthesenescenceprocess
22%inhibitionprogerin
production
TT2peptide
EffectofTT2peptide ondermalstructureandconnectivetissueorganization
MMPs
Elastase,collagenase
Dermis contraction
Syndecan
Progerin
MMP1,MMP3&MMP9inhibition
1
Progelinecontrol Progelinecontrol
T0 After24h
+27%collagen contraction
TT2peptide TT2peptide
EffectofTT2peptide ondermalstructureandconnectivetissueorganization
MMPs
Elastase,collagenase
Dermis contraction
Syndecan
Progerin
1
Control +collagenase +collagenase +Progeline
Collagenfibers
43%inhibitioncollagenase
TT2peptide
100%inhibitionelastase
Elastinfibers
Control +Elastase +Elastase +ProgelineTT2peptide
13
EffectofTT2ondermalstructureandconnectivetissueorganization
MMPs
Elastase,collagenase
Dermis contraction
Syndecan
Progerin
Bymodulangsenescenceprocess(progerin),
TT2peptide inducesseveralbiological
responsesatthedermis&epidermislevels
1
ClinicalStudiesFirmness,Laxity
1
Clinicalevaluationofantisaggingeffectonjawlines
TT2peptide decreasessignificantlythevolumeofthejawlinesafter28and56days
Protocol
10healthyvolunteers :5464yearsoldwithagedskin Formulation:2%TT2peptide emulsion creamorplacebo Application:splitface andsplitneck 2X/dayfor56days Measurementofthevolumeofthejawlines ofthefacebyinvivo
fringeprojectionprofilometry
80%ofvolunteers had animprovement
1
TT2peptide2%
Clinicalevaluationoffirmness&elasticityCutometryProtocol
13healthyvolunteers(54 66yearsold)withsaggyskin(lossoffirmness,slackeningskin) Formulation:2%TT2peptideemulsioncreamorplacebo Application:splitface andsplitneck 2X/dayfor28days Measurementsareperformedonbothcheeks(diameterprobe=2mm)bycutometry
1
Improvement upto: 93%ofcutaneous elasticity 82%ofcutaneous firmness 42%ofcutaneous viscoelasticity
TT2peptide increasessignificantlyfirmness,elasticityandviscoelasticityafter28days
TT2peptide2%
D0
Slight improvement
Majorbuildingeffect overtime!
Clinicalevaluationoffirmness&elasticity 1
TT2peptide
Summary TT2peptideeffect 1
SyndecanDermis contractionElastase,collagenasesProgerine
LaxityFirmness,Elasticity
Reverseeffect ofTT2peptide
Ourstrategytoreverseskinaging
DermislevelECMcomponent(collagens,elastin..)
MMPsactivitiesImmunefunctions
Low
Stratumcorneum CompactFlakyEpidermis
Thickness =properskinbarrierKeratinocytes,Mc,proliferation,maturation
Proteoglycanssynthesis
Thick Thin
++++++
FlatDermal Epidermal junctionDermis/epidermiscohesion
Wavy
Dermis
Epidermis
Hypodermis
SC
DEJ
HypodermislevelSubcutaneousfat
+++
+++
+++
+++
1 2
Youngskin
Fullfatcompartments
Matureskin
Deflatedfatcompartments
Nasolabialwrinkles
Structural agingduetolipoatrophy 2
Hormesis concept 2
Concept launched by Givenchy with Vaxin (2010) :This vaccinelike serum is formulated with a unique complex capable ofgenerating microstimuli, which encourage the skin's cells to produce theirown agedefying protein HSP70
The word hormesis comes from the greek root hormeticos, which meansstimulation and the first mention of the term hormesis in the open scientificliterature was published in 1943
- ++
++
Hormesis : homeostatic and adaptative beneficial response of cells to microstressexposure in order to correct a physiological imbalance
Activationofadipocyteanabolism
Adipogenesisactivation
(adipocytedifferenciation)
LipolysisInhibition
(freefattyacidrelease)
Lipogenesisactivation
(triglyceridesynthesis)
Adiposetissuegrowth
HIF1
+
Lipohormesis Newproprietarypatentedconcept 2
Lipohormesis = hormesis applied to adipose tissue
Various stresses in the adipose tissue are able to stimulate its expansion through theactivation of Hypoxia Inducible Factor (HIF1), a transcription factor
ReverseagingWrinklereduction
Plumperskin
Youngskin
Matureskin
L.O. Alipofillinglikealternative 2 LO(INCI:Ornithine(and)Phospholipids(and)Glycolipids):Lornithine,anaminoacid
obtainedbybiotechnologyfromvegetablestarch,encapsulatedinaIonosome
Ionosome: new generation of delivery system (proprietary patented). Polysaccharidecoated encapsulation: polysaccharides (stearoyl inulin) inserted in the
phospholipid membrane = protective coating
Ionosome benefits: Resistantdeliverysystem Highpenetrationcapacitytotargetadipocytes Higherefficacy
Lipophilicside
Hydrophilicside
C18
C18
C18
C18
L-ornithine
LO
LOreproduceslipohormesis phenomenonandmimics lipofilling result byincreasingfatty tissuevolumewith aglobalaction
2EffectofLOontheactivationofHIF1andadipocyteanabolismProtocol Human preadipocytes were isolated from biopsiesandcultivated fordifferentiation. 0.02%LO was added onadipocytesculture Genic expressionwas evaluated through cDNA methodology
ResultsAdipofill
HIF1
genes involvedinadipogenesis
genes involved inlipolysis
genes involvedinlipogenesis
Adipocytedifferenciation
Freefatty acidrelease
Triglyceridesynthesis
LOinhibitslipolysisanddecreasesthereleaseoffattyacidsfromadipocytesbyincreasinglipidstorage
Protocol
Lipidstorageenhancement lipolysis 2
Increase insize
Increase inlipid content
Humanadipocyteobtainedfromapieceofsurgicalresectionwereincubatedwith0.05%and0.2%LO Free fatty acids (NEFA) were quantified in the media using a spectrophotometric method and the adipocyte
size was evaluated through the measurement of their average diameter after staining with red oil (specific forlipids)
LO
LO
ClinicalStudiesNasogenian fold
2
Protocol 20healthyvolunteers(40 60yearsold)withnasogenian folds. Formulation:2%LOemulsioncreamorplacebo Application:splitfaceandsplitneck2X/dayfor56days Measurementofdepth androughness byprofilometry andevaluationofsmoothness byadermatologist
Reductionofnasogenian fold 2
85% of volunteers hadan improvement
LOstronglyreducestheappearanceofnasogenian foldafteronlyonemonth
Reduction upto: 27.5%ofroughnesss 37.6%ofwrinkle depth
LO2% LO2%
Reductionofnasogenian fold 2
D0
D60
LOstronglyreducestheappearanceofnasogenian fold
Summary LOeffect 2
HIF1AdipogenesisLipogenesisLipolysisAdiposetissue
Nasogenian fold
Reverseeffect ofLO
TT2peptide
21LO
+
Bymodulangsenescenceprocess(progerin),
TT2peptide targetsDERMIS restructurationfora
remodellingeffectandwrinklesreduction
Byinducinglipohormesis(HIF1),LO targetsHYPODERMIS restructurationfora
lipofilling effectandnasogenian foldreduction
21
4LaSpiritCream
forasynergisticefficacy
LaSpiritCream
TT2peptide:Improves firmness &reduces wrinkles andsagging
LO: Reversesagerelated lipoatrophy toplump theskin
UNIQUE, SOPHISTICATED AND BIO-EFFICIENT ACTIVE INGREDIENTSAND DELIVERY SYSTEMS
DELICIOUS TEXTURES THATDELIVER EXQUISIT SKIN FEEL
Hydrogenated Lecithin (and)C1216Alcohols (and)Palmitic acid:Brings the
phospholipids touch (coolness,softness)&comfort
SodiumAcrylatesCopolymer (and)Lecithin:Brings alighttexturetothe
emulsion
SKINBENEFITS AFTER1WEEK
AFTER4WEEKS
Theproductispleasant toapply 100% 100%
Thetextureissmooth 96% 97%
Theskinfeelssofter 92% 97% 87% IRISDATABASE*
Theskinfeelssuppler 86% 91% 89%
Theskinseemshydrated 85% 87% 84%
Theskinismoreluminous 71% 73% 73%
Theskinlooksmoreuniform 67% 79% 77%
Thesignsoffatigue arelessvisible 69% 75%
Theskintexture looksimproved 67% 75%
Theskinfeelsfirmer 63% 73%
Theskinhaveahealthyglow 56% 72%
Theskinlooksvisiblysmooth out 51% 70%
LaSpiritCreamTested by62women (45to70)twice aday for28day
Compared toIris
historical result on
antiaging consumer
tests2567tests
Conclusion
Focusonstructuralaging
YoungSkin MatureSkin
Reverseeffect ofTT2peptide&LO
Cutaneous aging characterized by a loss of elasticity, appearance of wrinkles
Structural aging due to a lipoatrophy (loss of adipose tissue)
Cellularagingwithaprogressivedysfunctionofthecellmetabolism(progerin)
JeanneCalment (1875 1997)
ThankYou!
Livingtotheageof122years=44724days