The Nitrogen CycleThe Nitrogen Cycle

Why is nitrogen so important?Why is nitrogen so important?

• Nitrogen is required to make PROTEINSPROTEINS

Amino Acid Structure

• Proteins are found everywhere in living organisms (plants and animals)– Muscles– DNA– Cell membranes– Enzymes

Where is nitrogen found Where is nitrogen found in the environment?in the environment?

• The largest single source of nitrogen is the atmosphere– Nitrogen (N2)

makes up 79%79% of the air we breathe.

The Problem:The Problem:

• Most living organisms are unable to use nitrogen as it exists in the atmosphere (as N2).

• To enter the food chain, nitrogen must be converted

into nitratesnitrates (NO (NO33-1-1)) which

can easily be absorbed byplants to make DNA andPROTEINS.

-1

The Solution:The Solution:Nitrogen FixationNitrogen Fixation

• Nitrogen FixationNitrogen Fixation is the process that causes the strong bonds in the N2 molecules to break apart. This requires energy.

• Nitrogen is said to be fixedfixed when nitrogen combines with oxygen, forming nitrate ions (NO3

-1)

• Lightning and nitrogen fixing bacteria are responsible for this process.

Nitrogen + Oxygen + Energy Nitrates

Nitrogen Fixation by LightningNitrogen Fixation by Lightning

• The energy produced by lightning splits nitrogen gas (N2) in the atmosphere allowing it to react with oxygen (O2) to form nitrates

• Nitrates dissolve in rain or surface water and enter the soil where it is absorbed by plants through their roots.

Nitrogen Fixation by BacteriaNitrogen Fixation by Bacteria

Nitrogen Fixing Bacteria:Nitrogen Fixing Bacteria:

• found in the soil or on plant roots of legumes (clover, soybean, peas and alfalfa).

• require high levels of oxygen to fix nitrogen.

• fix nitrogen more often than lightning.

nodule

Nitrogen Movement throughNitrogen Movement throughthe Food Chainthe Food Chain

• Once nitrates are absorbed by plants, the nitrogen becomes part of the plant tissue as proteins, DNA, enzymes, etc.

• Herbivores consume the plants and carnivores consume the animals, all of which have nitrogen stored in their bodies.

What happens to nitrogen when What happens to nitrogen when plants and animals die or plants and animals die or

produce waste?produce waste?

• Decomposers break down the dead material and waste and produce ammonia (NH3).

• Ammonia is also produced by the waste/dead material itself.

• Nitrifying bacteria in the soil combine ammonia (NH3) with oxygen (O2) to form nitrites (NO2

-1)

• Another type of nitrifying bacteria then add more oxygen to the nitrites to form nitrates (NO3

-1).

• These nitrates can then be absorbed by plants through their roots.

Ammonia + Oxygen Nitrites Nitrates

NitrificationNitrification

from the decompositionof dead plants, animals and waste

Nitrification

How is nitrogen cycledHow is nitrogen cycledback into the atmosphere?back into the atmosphere?

Denitrification:Denitrification:• Occurs when oxygen

levels in the soil are low, such as in marches, swamps and bogs.

• Denitrifying Bacteria convert nitrates back into nitrogen gas which returns to the atmosphere.

ReviewReviewName and describe the three (3) processes by which nitrogen is cycled:

1. Nitrogen FixationNitrogen Fixation – forms nitrates from nitrogen gas (N2) in the atmosphere via:

Lightning Nitrogen Fixing Bacteria (requires O2)

2. NitrificationNitrification – forms nitrates from ammonia (dead plants, animals and waste) via:

Nitrifying Bacteria (requires O2)

3. DenitrificationDenitrification – forms nitrogen gas (N2) from nitrates via: Denitrifying Bacteria (does not require O2)

Human Impact on theHuman Impact on theNitrogen CycleNitrogen Cycle

Excessive use of fertilizers has added

lots of nitrates (NO3-1) to the soil, leading to:

1. 1. EutrophicationEutrophication

– Nitrates travel in the spring runoff and cause algae to grow rapidly in lakes and ponds.

– Bacteria that decompose dead algae consume oxygen, causing less oxygen to be available for aquatic organisms death

Human Impact on theHuman Impact on theNitrogen Cycle (cont’d)Nitrogen Cycle (cont’d)

2. 2. Acidic Soil and WaterAcidic Soil and Water– Nitrates react with water (in lakes, ponds and

soil) to produce Nitric acid– Crops fail to prosper because they cannot

handle the change in pH of the soil– Decreasing pH of water causes aquatic

organisms to die

The Nitrogen Cycle

Lowoxygen

HighOxygen

Questions: pg. 69 #4,6,8,9Questions: pg. 69 #4,6,8,9

4. Nitrogen fixing bacteria are found in the 4. Nitrogen fixing bacteria are found in the roots of bean plants. Explain how the roots of bean plants. Explain how the bacteria benefit the plant and how the plant bacteria benefit the plant and how the plant benefits the bacteria.benefits the bacteria.

• Bacteria create the nitrates so that the plant can grow.

• The plant makes glucose which is consumed by the bacteria so it can get energy to live (respiration).

• The relationship between the bacteria and the plant is called mutualismmutualism (meaning, they both benefit each other)

6.6. Explain why it is a good practice to Explain why it is a good practice to aerate lawns.aerate lawns.

• When you aerate your lawn, you are mixing up the soil so that there is more oxygen in it.

• This increases the action of the nitrogen fixing bacteria that require oxygen. This leads to:– Increased amounts of nitrate entering the soil

which is good for plants.

8. Some farmers alternate 8. Some farmers alternate crops that require rich crops that require rich supplies of nitrogen, supplies of nitrogen, such as corn, with such as corn, with alfalfa. However, alfalfa. However, alfalfa is usually less alfalfa is usually less valuable in the valuable in the marketplace than marketplace than corn. Why would corn. Why would farmers plant a crop farmers plant a crop that provides less that provides less economic value?economic value?

• Alfalfa roots contain Nitrogen Fixing Bacteria which will produce plenty of nitrates for the corn.

• By using alfalfa, the corn that grows will be more hearty, and will be easier to sell compared to a lot of corn that is not as hearty.

9.9. Explain why bogs and swamps are usually Explain why bogs and swamps are usually low in nitrogen.low in nitrogen.

• Bogs and swamps contain a lot of denitrifying bacteria.

• This means that a lot of nitrate is converted into nitrogen gas, leaving very little nitrate for the plants that live in the area.

• Since plants are not receiving nitrates from this source, some have evolved over time to have the ability to consume insects.

Insect-eating pitcher plant

Your Task:

Complete questions #1-3 on pg. 69

Pictures from:

• http://www.proteomesoftware.com/Proteome_software_ed_Proteins.html

• http://www.cookstown.gov.uk/business/environmentalhealth/environmentalprotection/

• http://reference.findtarget.com/search/nitrogen/• http://www.marietta.edu/~biol/biomes/images/mangroves

/florida_eutrophication_7536.jpg

• http://www.realgreenlawn.com/austin_texas/images/design_elements/Coreaeriation.jpg

• http://www.gardenaerator.co.uk/aerator-types.html