The NucleoCounter® NC-100™Fast, precise and objective counting andviability determination of mammalian cells

NucleoCounter® NC-100™ key advantages and selling points

Easy operation – no preparation 30 sec. analysis time Calibration free No cleaning Maintenance and service free Portable/compact Safe sample handling and disposal High precision & reproducibility

NucleoCounter® NC-100™ - Mammalian cells

NucleoCounter is optimized to count mammalian cells cultured in TC-flasks, bioreactors, micro carriers etc.

NucleoCounter® NC-100™ Concept: 5 components

NucleoCounter® NC-100™

NucleoCassetteLysis Buffer

Stabilizing BufferNucleoView Software

Easy Operation

Lyse Load Count

Propidium Iodide

1 dye molecule per 4-5 bp

Genome between 2.5 x 109 – 4.0 x 109 bp.

DNA base pair (bp):

A: Adenine

T: Thymine

G: Guanine

C: Cytosine

C27H34N42+.2I- Mr 668.4

Limitations of present methods of cell counting

Cell Counting, Whole Nuclei Using Fluorescent DNA Dye PI

Hard to discriminate cells from debris by microscopy

Easy to count nuclei by fluorescence microscopy

Integrated Fluorescence Microscope

Lenses: magnification <1X. Excitation filter: sorts out green light. Emission filter: sorts out long wave length including red light.

fixture

Integrated Fluorescence Microscope

The NucleoCassette™

Nuclei of Mammalian cells

Different in cell size:Diameter between 20-40 m.

Nuclei are almost uniform in size:10-12 m.Genome between 2.5x109 – 4.0x109 bp.

Advantage: No calibration to cell type (size) using the NucleoCounter NC-100.

Reagent A-100 and B

Lysis reagent A-100:

Disrupts the plasma membranes of the mammalian cells, rendering the

nuclei susceptible to staining with PI

Disaggregation of cell clusters

Stabilizing reagent B:

Raises the pH value, allowing PI to stain the DNA of the cells more efficiently

Viability Determination

NucleoCounter® NC-100™ comparison with conventional cell counting methods

NucleoCounter® NC-100™ comparison with conventional cell counting methods

NucleoView Software

Technical Specifications

Validation IQ/OQ

Typical NC-100 customers

NucleoCounter® NC-100™ key advantages and selling points

Easy operation – no preparation 30 sec. analysis time Calibration free No cleaning Maintenance and service free Portable/compact Safe sample handling and disposal High precision & reproducibility