The presentation you are about to view The presentation you are about to view contains the contains the

published mechanisms of published mechanisms of immune suppression associated with immune suppression associated with

OspA or the LYMErix vaccine.OspA or the LYMErix vaccine.

The Greatest ImitatorThe Greatest Imitator

Brought to you by ~ Concerned Brought to you by ~ Concerned CitizensCitizens

Overview of this OspA/Pam3Cys Overview of this OspA/Pam3Cys presentationpresentation

Because structure is function we have Because structure is function we have attempted to share what is known about attempted to share what is known about Pam3Cys or OspA:Pam3Cys or OspA: 1) History and structure,1) History and structure,2) Hypothetical structures of gram negative 2) Hypothetical structures of gram negative bacterial lipoproteinsbacterial lipoproteins3) Published research on immunosuppression 3) Published research on immunosuppression from fungal antigens (TLR2 vs TLR4)from fungal antigens (TLR2 vs TLR4)4) How and when the Yale/ALDF.com Lyme 4) How and when the Yale/ALDF.com Lyme cabal knew OspA/Pam3Cys wasn’t a vaccine cabal knew OspA/Pam3Cys wasn’t a vaccine (was not proven to prevent “Lyme Disease”).(was not proven to prevent “Lyme Disease”).

We can find no evidence that anyone We can find no evidence that anyone really knows what OspA looks likereally knows what OspA looks like

Recall from the Powerpoint Presentation on Scientific Recall from the Powerpoint Presentation on Scientific Validity, that you can’t crystallize a lipid (fat, oil, Validity, that you can’t crystallize a lipid (fat, oil, fatty acid, margarine, motor oil, hydrogenated-like fatty acid, margarine, motor oil, hydrogenated-like grease…).grease…).

So, since Pam3Cys or OspA or tripalmitoyl cysteine or So, since Pam3Cys or OspA or tripalmitoyl cysteine or mycoplasmal and spirochetal lipoproteins are mycoplasmal and spirochetal lipoproteins are LIPOproteins, it’s almost impossible to see the real LIPOproteins, it’s almost impossible to see the real structure, especially of the free antigens (and not structure, especially of the free antigens (and not their membrane-bound arrangement of atoms).their membrane-bound arrangement of atoms).

John Dunn at BrookhavenJohn Dunn at BrookhavenDepartment of Energy ReportDepartment of Energy Report [SUBROUTINE JD@BHDOE, slide 1][SUBROUTINE JD@BHDOE, slide 1]

Untangling the Structure of Lyme DiseaseUntangling the Structure of Lyme Disease by Michaela Mann, ENERGY SCIENCE NEWSby Michaela Mann, ENERGY SCIENCE NEWS

““The Department of Energy's National Synchrotron The Department of Energy's National Synchrotron Light Source at Brookhaven National Laboratory Light Source at Brookhaven National Laboratory helped researchers discover new information about helped researchers discover new information about the bacterium that causes Lyme disease. Their work the bacterium that causes Lyme disease. Their work may lead to an effective vaccine and new treatment may lead to an effective vaccine and new treatment protocols. protocols.

“ “Ixodes scapularis (deer ticks) are the most common Ixodes scapularis (deer ticks) are the most common vector for Lyme disease. Larval and nymphal ticks are vector for Lyme disease. Larval and nymphal ticks are no bigger than the eye of a common sewing needle. no bigger than the eye of a common sewing needle. Adult ticks are about the size of a small apple seed.Adult ticks are about the size of a small apple seed.

John Dunn at BrookhavenJohn Dunn at BrookhavenDepartment of Energy ReportDepartment of Energy Report

[SUBROUTINE JD@BHDOE, slide 2] [SUBROUTINE JD@BHDOE, slide 2]

"It's the perfect stealth bacteria," says one "It's the perfect stealth bacteria," says one frustrated physician. He's talking about Borrelia frustrated physician. He's talking about Borrelia burgdorferi, the bacterium that causes Lyme burgdorferi, the bacterium that causes Lyme disease. This illness, which is often mistaken for disease. This illness, which is often mistaken for diseases ranging from diseases ranging from multiple sclerosis to multiple sclerosis to Lupus, can inflict excruciating headaches and Lupus, can inflict excruciating headaches and muscle pain, affect the brain and nervous muscle pain, affect the brain and nervous systemsystem, attack major organs, and inflame joints. , attack major organs, and inflame joints. Although there have been more than 100,000 Although there have been more than 100,000 reports of the tick-borne Lyme disease in the U.S. reports of the tick-borne Lyme disease in the U.S. since 1982, researchers are still struggling to create since 1982, researchers are still struggling to create vaccines and treatments that are effective against vaccines and treatments that are effective against B. burgdorferi. B. burgdorferi.

John Dunn at BrookhavenJohn Dunn at BrookhavenDepartment of Energy ReportDepartment of Energy Report[SUBROUTINE JD@BHDOE, slide 3][SUBROUTINE JD@BHDOE, slide 3]

““New findings may explain vaccine failure, New findings may explain vaccine failure, suggest treatment approaches Investigators suggest treatment approaches Investigators are particularly pleased with two recent are particularly pleased with two recent discoveries made using the Department of discoveries made using the Department of Energy's National Synchronous Light Source Energy's National Synchronous Light Source (NSLS) at Brookhaven National Laboratory. (NSLS) at Brookhaven National Laboratory. The uniquely refined images they were able The uniquely refined images they were able to create demonstrated the to create demonstrated the bacterium bacterium changes its outer surface protein changes its outer surface protein according to its hostaccording to its host, and that different , and that different strains of the bacterium have different strains of the bacterium have different electrical charges, which may determine electrical charges, which may determine their ability to cause disease.”their ability to cause disease.”

John Dunn at BrookhavenJohn Dunn at BrookhavenDepartment of Energy ReportDepartment of Energy Report[SUBROUTINE JD@BHDOE, slide 4] [SUBROUTINE JD@BHDOE, slide 4]

““Understanding the structure is the keyUnderstanding the structure is the keyThe new understanding of the structure was The new understanding of the structure was made possible by the protein fixation and made possible by the protein fixation and imaging techniques at NSLS. The NSLS imaging techniques at NSLS. The NSLS permits researchers to focus and control light permits researchers to focus and control light beams such that images can be seen at beams such that images can be seen at resolutions as fine as 2 A-near atomic resolutions as fine as 2 A-near atomic resolution.resolution.

““It is no easy matter to concoct fragile It is no easy matter to concoct fragile organic matter, such as protein chains, into organic matter, such as protein chains, into crystals that can withstand the powerful crystals that can withstand the powerful radiation bombardment of the NSLS and yet radiation bombardment of the NSLS and yet retain their original structure. retain their original structure.

John Dunn at BrookhavenJohn Dunn at BrookhavenDepartment of Energy ReportDepartment of Energy Report[SUBROUTINE JD@BHDOE, slide 5][SUBROUTINE JD@BHDOE, slide 5]

““To do this, the Brookhaven team drew upon To do this, the Brookhaven team drew upon available nuclear magnetic resonance (NMR) available nuclear magnetic resonance (NMR) information to identify the least stable areas information to identify the least stable areas of the of the OspCOspC protein—the C and N termini. protein—the C and N termini. They truncated the protein to They truncated the protein to removeremove these these termini and improve their chances of termini and improve their chances of crystallizing portions of the protein into a crystallizing portions of the protein into a stable, viewable form. They then expressed stable, viewable form. They then expressed and purified the protein to ensure and purified the protein to ensure homogeneity, and grew them as homogeneity, and grew them as crystals.”crystals.”

SillySilly

The truth is that this is nothing new, and that there was The truth is that this is nothing new, and that there was never a “key to vaccine failure” until we learned about never a “key to vaccine failure” until we learned about immune suppression due to synthetic Pam3Cys or the immune suppression due to synthetic Pam3Cys or the OspA vaccines. Relapsing Fever was OspA vaccines. Relapsing Fever was alwaysalways known to known to be Relapsing Fever, that the protein ends of the be Relapsing Fever, that the protein ends of the lipoproteins underwent “antigenic variation” or lipoproteins underwent “antigenic variation” or “antibody selection pressure,” that the nature of “antibody selection pressure,” that the nature of relapses is that antibodies, and vaccines do no good due relapses is that antibodies, and vaccines do no good due to the to the antigenic variation.antigenic variation.

But But ANYWAY…ANYWAY… ThereThere was Brookhaven unable to re- was Brookhaven unable to re-crystallize OspA in order to shoot it with X-Rays because crystallize OspA in order to shoot it with X-Rays because you can’t freeze lipids. They don’t have the real you can’t freeze lipids. They don’t have the real structure.structure.

X-Ray Diffraction and Generating an X-Ray Diffraction and Generating an ImageImage

Structure of Tripalmitoyl Cysteine Structure of Tripalmitoyl Cysteine (Bulletin of Korean Chem Society, (Bulletin of Korean Chem Society,

1996, Vol. 17, No. 11)1996, Vol. 17, No. 11)

A synthetic lipopeptideA synthetic lipopeptide

Tri - Palmitic - AcidTri - Palmitic - Acid

The curious case of HIV antibodies The curious case of HIV antibodies against Pam3Cys PMID 2464607 against Pam3Cys PMID 2464607

1988 1988 Distinction between HIV-1 and HIV-2 infection using novel Distinction between HIV-1 and HIV-2 infection using novel synthetic lipopeptide conjugates as antigens in enzyme immunoassays.synthetic lipopeptide conjugates as antigens in enzyme immunoassays.

““A novel immunoassay technique using synthetic A novel immunoassay technique using synthetic lipopeptide (Pam3Cys-Ser) linked to lipopeptide (Pam3Cys-Ser) linked to immunodominant peptide domains of HIV-1 and immunodominant peptide domains of HIV-1 and HIV-2 envelope proteins as an antigen adsorbent HIV-2 envelope proteins as an antigen adsorbent has been developed. Attachment of peptides to has been developed. Attachment of peptides to microtiter plates can be considerably improved microtiter plates can be considerably improved with this method by employing the hydrophobic with this method by employing the hydrophobic properties of lipopeptide. properties of lipopeptide. From the sera of 121 From the sera of 121 HIV-1 infected patients 117 reacted with HIV-1 infected patients 117 reacted with Pam3CysPam3Cys-Ser-[HIV-1(598-609)cyclic disulfide]. -Ser-[HIV-1(598-609)cyclic disulfide]. Five of 5 HIV-2 positive sera were positive with Five of 5 HIV-2 positive sera were positive with Pam3Cys-Ser-[HIV-2(593-603)cyclic disulfide]. Pam3Cys-Ser-[HIV-2(593-603)cyclic disulfide]. Control sera failed to react with these conjugates.”Control sera failed to react with these conjugates.”

Pam3Cys structure, again, on a hypothetical HIV vaccine, Pam3Cys structure, again, on a hypothetical HIV vaccine, Defoort, et al, PubMed #1478779, 1992… ????Defoort, et al, PubMed #1478779, 1992… ????

Slide 16Slide 16-- Pam3Cys - structure first found -- Pam3Cys - structure first found on the web in a on the web in a

Korean Chemistry JournalKorean Chemistry Journal (We have (We have since found it elsewhere).since found it elsewhere).

The Korean Chemists say…The Korean Chemists say…

Analog of E. coli "lipid A" PubMed # 3281910

Pam3Cys is synthetic (Manmade; is Pam3Cys is synthetic (Manmade; is analogous to)analogous to)

E. coliE. coli Lipid A Lipid A

WiesmWiesmüüller, 1983, synthesizing ller, 1983, synthesizing Pam3Cys, PubMed ID 6347861Pam3Cys, PubMed ID 6347861

Slide 21 -- Through the years, we were trying to find Slide 21 -- Through the years, we were trying to find background data on this structure (function). This background data on this structure (function). This report is from Schrreport is from Schrööder and Schumann, 2004… der and Schumann, 2004… PubMed ID# 15294986PubMed ID# 15294986

What do these membrane components do? What do these membrane components do? Stealing a graphic from Duke (Raetz) on Stealing a graphic from Duke (Raetz) on E. E.

colicoli membrane structure membrane structurehttp://www.biochem.duke.edu/faculty/christian-raetz/research-interestshttp://www.biochem.duke.edu/faculty/christian-raetz/research-interests

Zooming in on the E. coli Zooming in on the E. coli membrane (Duke)membrane (Duke)

The Best Scientist Sources - The Best Scientist Sources - 11

It’s only been in the past few years since It’s only been in the past few years since ActionLyme summarized the immune ActionLyme summarized the immune suppression data available on Pam3Cys suppression data available on Pam3Cys and on how the Tuberculosis vaccines, as and on how the Tuberculosis vaccines, as well as the Lyme vaccines failed. Since well as the Lyme vaccines failed. Since mid-2003 to the fall of 2005, when mid-2003 to the fall of 2005, when lipoprotein vaccines and Pam3Cys related lipoprotein vaccines and Pam3Cys related immune suppression was revealed by immune suppression was revealed by ActionLyme, there has been more interest ActionLyme, there has been more interest in this phenomenon. Therefore there is in this phenomenon. Therefore there is more data more data nownow, in 2009., in 2009.

Best Science/Scientist Sources Best Science/Scientist Sources - 2- 2

The best sources (recommended by ActionLyme) are The best sources (recommended by ActionLyme) are Justin Radolf, Janis Weis, SchrJustin Radolf, Janis Weis, Schrööder and Schumann, der and Schumann, WiesmWiesmüüller and his group in Germany, and now Duke ller and his group in Germany, and now Duke Biochemistry Department. (There are also jobs out Biochemistry Department. (There are also jobs out there for Lipid Biochemists, we noticed.)there for Lipid Biochemists, we noticed.)

This is a hot field This is a hot field nownow, because everyone sort of , because everyone sort of found out that Yale found out that Yale LIEDLIED about their LYMErix vaccine about their LYMErix vaccine outcome. OspA did not prevent Lyme. It caused outcome. OspA did not prevent Lyme. It caused Chronic Pam3Cys or OspA-Immune-Suppression Chronic Pam3Cys or OspA-Immune-Suppression Syndrome, and the New Great Imitators.Syndrome, and the New Great Imitators.

Slide 26, Continuing from Slide – 21; Slide 26, Continuing from Slide – 21; Lipopolysaccharide Binding Protein Binds to Lipopolysaccharide Binding Protein Binds to Triacylated and Diacylated Lipopeptides and Triacylated and Diacylated Lipopeptides and

Mediates the Immune ResponsesMediates the Immune Responses, 2004 Schr, 2004 Schröder, öder, 15294986[uid]15294986[uid]……

““Lipoproteins and lipopeptides have been identified Lipoproteins and lipopeptides have been identified in a largein a large number of microorganisms, the most number of microorganisms, the most prominent ones being prominent ones being mycobacteria,mycobacteria,

mycoplasms, and spirochetesmycoplasms, and spirochetes. They have been . They have been found to exhibitfound to exhibit both a strong innate inflammatory both a strong innate inflammatory response in the host and anresponse in the host and an enduring adaptive enduring adaptive immune response in mammalian hosts (immune response in mammalian hosts (1616). The). The

strong proinflammatory capacities of lipoproteins strong proinflammatory capacities of lipoproteins were firstwere first described for outer surface proteins described for outer surface proteins A A and B of Borrelia burgdorferiand B of Borrelia burgdorferi,, which are also which are also highly…”highly…”

Continuing backgroundContinuing background 15294986[PMID]15294986[PMID]

“…“…immunogenic (immunogenic (1717) and have lately been) and have lately been the the basis for a Lyme disease vaccine development (basis for a Lyme disease vaccine development (1818). These). These compounds exhibit an triacylated lipid compounds exhibit an triacylated lipid anchor structure comprisinganchor structure comprising an N-palmitoyl-S-an N-palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-(R)-cysteinyl[2,3-bis(palmitoyloxy)-(2RS)-propyl]-(R)-cysteinyl

((PamPam33CysCys) moiety at the N terminus () moiety at the N terminus (1919), a ), a

feature that wasfeature that was previously described for the previously described for the Braun lipoprotein from EscherichiaBraun lipoprotein from Escherichia coli (coli (2020). ). Because the N-terminal PamBecause the N-terminal Pam33Cys modification is Cys modification is

essentialessential for immunoactivation caused by for immunoactivation caused by lipoproteins of B. burgdorferilipoproteins of B. burgdorferi as well as of another as well as of another spirochete, Treponema pallidum (spirochete, Treponema pallidum (2121),…”),…”

Continuing background Continuing background 15294986[PMID] on what this 15294986[PMID] on what this

compound is…compound is… “…“…subsequentsubsequent studies investigating immune studies investigating immune

responses to spirochetes used syntheticresponses to spirochetes used synthetic

lipopeptides (lipopeptides (2222). The ). The PamPam33CysCys moiety was moiety was also reported to bealso reported to be present in cytokine-present in cytokine-inducing lipoproteins of Mycobacterium inducing lipoproteins of Mycobacterium andand Mycoplasma spp. (Mycoplasma spp. (2323, , 2424);); thus, it can thus, it can be regarded as a highlybe regarded as a highly conserved molecular conserved molecular motif among different classes of bacteria.motif among different classes of bacteria. In In Mycoplasma fermentans, the presence of a Mycoplasma fermentans, the presence of a macrophage stimulatingmacrophage stimulating lipopeptide, termed lipopeptide, termed 2-kDa macrophage-activating lipopeptide2-kDa macrophage-activating lipopeptide

((MALP-2MALP-2),…” ),…”

Once the Once the ActualActual Scientists realized that Scientists realized that the Tuberculosis vaccines failed the Tuberculosis vaccines failed

because they were lipoproteins…because they were lipoproteins…

ActionLyme reported a series of research articles on the ActionLyme reported a series of research articles on the failures of the Tuberculosis lipoprotein vaccines (19kD, failures of the Tuberculosis lipoprotein vaccines (19kD, 27kD) in 2003. They all seemed to fail in the exact 27kD) in 2003. They all seemed to fail in the exact same way LYMErix appeared to fail, which was (as same way LYMErix appeared to fail, which was (as reported by KMDickson to the FDA Vaccine Committee in reported by KMDickson to the FDA Vaccine Committee in January, 2001), in that January, 2001), in that it appeared to make the it appeared to make the perhaps existing infection(s) worse.perhaps existing infection(s) worse.

[UConn’s Justin Radolf and Yale’s Erol Firkig are [UConn’s Justin Radolf and Yale’s Erol Firkig are Actual Actual Scientists; Scientists; although they’re the although they’re the onlyonly ones in Connecticut. But they’re ones in Connecticut. But they’re notnot squawking about these crimes. So we wonder what that mentality squawking about these crimes. So we wonder what that mentality is, where you know stuff is scientific BS but you don’t say anything is, where you know stuff is scientific BS but you don’t say anything publicly. And if we can’t find the DSM entry for that “Does Not publicly. And if we can’t find the DSM entry for that “Does Not Speak to the Scientific BS” mental illness, we will make it up and Speak to the Scientific BS” mental illness, we will make it up and submit it.]submit it.]

Continuing intro for SchrContinuing intro for Schröder, et al, öder, et al,

PubMed ID# 15294986PubMed ID# 15294986

“…“…was observed, being stimulatory active was observed, being stimulatory active at picomolarat picomolar concentrations (concentrations (2525). This ). This compound, in contrast to the predominantcompound, in contrast to the predominant

lipopeptide structures present in lipopeptide structures present in lipoproteins of E. coli, B.lipoproteins of E. coli, B. burgdorferi, and burgdorferi, and mycobacteria, lacks the N-palmitoyl group,mycobacteria, lacks the N-palmitoyl group,

thus containing a thus containing a diacylated (Pamdiacylated (Pam22Cys)Cys)

lipid anchor structurelipid anchor structure at the N terminus. at the N terminus. Following studies revealed the presence ofFollowing studies revealed the presence of

closely related compounds in other closely related compounds in other Mycoplasma spp. (Mycoplasma spp. (2626).”).”

2004, Schr2004, Schrööder, 15294986[uid] der, 15294986[uid] continuedcontinued

““The innate immune system is represented by both The innate immune system is represented by both humoral andhumoral and cellular elements (cellular elements (11, , 22). Soluble CD14 ). Soluble CD14 (sCD14)(sCD14)44 and LPS binding and LPS binding protein (LBP) are major serum protein (LBP) are major serum factors with the ability to bindfactors with the ability to bind pathogens and initiate pathogens and initiate innate immune responses (innate immune responses (33, , 44). The cellular,). The cellular, so-called so-called pattern recognition receptors include the recentlypattern recognition receptors include the recently

identified family of TLRs (identified family of TLRs (22). ). TLRs are germline-TLRs are germline-encoded receptorsencoded receptors exhibiting homologies to Toll, exhibiting homologies to Toll, which is involved in embryogenesiswhich is involved in embryogenesis and host and host defense against Gram-positive bacteria and fungi defense against Gram-positive bacteria and fungi inin Drosophila (Drosophila (55, , 66).). TLR-2 was originally TLR-2 was originally described to recognizedescribed to recognize LPS, a major constituent LPS, a major constituent of the outer membrane of Gram-negativeof the outer membrane of Gram-negative bacteria bacteria ((77, , 88), whereas later studies identified TLR-4 as ), whereas later studies identified TLR-4 as thethe central transmembrane component of the LPS central transmembrane component of the LPS receptorreceptor ( (99, , 1010).”).”

(continued, 15294986[uid])(continued, 15294986[uid])

““The reason for this discrepancy was that LPS The reason for this discrepancy was that LPS preparations usedpreparations used contained contained contaminating bacterial contaminating bacterial lipoproteins,lipoproteins, which later which later were identified to were identified to be highly active TLR-2 agonists (be highly active TLR-2 agonists (1111, , 1212).). TLR-TLR-2 also has been shown meanwhile to interact 2 also has been shown meanwhile to interact with numerouswith numerous ligands of mainly bacterial ligands of mainly bacterial origin, including peptidoglycanorigin, including peptidoglycan and and lipoteichoic acid (LTA) of Gram-positive lipoteichoic acid (LTA) of Gram-positive bacteria (bacteria (1313, , 1414)) and spirochetal glycolipids (and spirochetal glycolipids (1414, , 1515).” ).”

““Contaminating bacterial Contaminating bacterial lipoproteins”lipoproteins”

““The reason for this discrepancy was that LPS The reason for this discrepancy was that LPS preparations usedpreparations used contained contained contaminating contaminating bacterial lipoproteins,bacterial lipoproteins, which later which later were were identified to be highly active TLR-2 agonists (identified to be highly active TLR-2 agonists (1111, , 1212).” ).” --15294986[uid]--15294986[uid]

References 11 and 12 refer to the works in 1999 of Radolf References 11 and 12 refer to the works in 1999 of Radolf and Weis as regards and Weis as regards Borrelia burgdorferi:Borrelia burgdorferi:

Toll-like Receptor 2 Functions as a Pattern Recognition Toll-like Receptor 2 Functions as a Pattern Recognition Receptor for Diverse Bacterial ProductsReceptor for Diverse Bacterial Products, 1999, Radolf, et al, PMID #10559223 , 1999, Radolf, et al, PMID #10559223

Cutting Edge: Inflammatory Signaling by Borrelia burgdorferi Cutting Edge: Inflammatory Signaling by Borrelia burgdorferi Lipoproteins Is Mediated by Toll-Like Receptor 2Lipoproteins Is Mediated by Toll-Like Receptor 21, 1999,1, 1999, Weis, Weis, PubMed ID #10452971 PubMed ID #10452971

The Plot Thickens…The Plot Thickens…

But the “contaminating compounds” were But the “contaminating compounds” were probably from mycoplasma.probably from mycoplasma.

We really don’t think everything is contaminated We really don’t think everything is contaminated with with Borrelia burgdorferi spheroplastsBorrelia burgdorferi spheroplasts, just because , just because antibiotic treatment forces these organisms into the antibiotic treatment forces these organisms into the spheroplast form (Barbour, 1982) which are spheroplast form (Barbour, 1982) which are regenerative (Everybody, including Russell Johnson, regenerative (Everybody, including Russell Johnson, Willy Burgdorfer and all the ALDF/Yale gang who Willy Burgdorfer and all the ALDF/Yale gang who referenced the URI research where “the referenced the URI research where “the spheroplasts regenerated into intact spirochetes spheroplasts regenerated into intact spirochetes within one minute of addition of whole rabbit within one minute of addition of whole rabbit blood,” not to mention Allen Steere’s “4/9 lab blood,” not to mention Allen Steere’s “4/9 lab workers” who apparently inhaled these airborne workers” who apparently inhaled these airborne cyst forms of spirochetes but “tested positive to the cyst forms of spirochetes but “tested positive to the Seronegative Lyme T – cell assay used by Allen Seronegative Lyme T – cell assay used by Allen Steere.”).Steere.”).

Steere and Steere and The T-cell proliferative The T-cell proliferative assay in the diagnosis of Lyme diseaseassay in the diagnosis of Lyme disease..

PUBMED ID: 1883122PUBMED ID: 1883122

Allen Steere’s “4/9 lab workers” apparently inhaled Allen Steere’s “4/9 lab workers” apparently inhaled these airborne cyst forms of spirochetes but these airborne cyst forms of spirochetes but “tested positive to the Seronegative Lyme T – cell “tested positive to the Seronegative Lyme T – cell assay used by Allen Steere.”assay used by Allen Steere.”

Feel free to go ahead and ask Allen Steere what he Feel free to go ahead and ask Allen Steere what he was talking about when he reported that:was talking about when he reported that:

The T-cell proliferative assay in the The T-cell proliferative assay in the diagnosis of Lyme disease.diagnosis of Lyme disease.

1982, Alan Barbour on what happens when 1982, Alan Barbour on what happens when you treat Relapsing Fever spirochetes with you treat Relapsing Fever spirochetes with

antibiotics:antibiotics:

Recall that in the Scientific Validity Recall that in the Scientific Validity Presentation we showed that All Borreliae Presentation we showed that All Borreliae are Relapsing Fever Organisms, that they are Relapsing Fever Organisms, that they are categorized by differences in their are categorized by differences in their flagellin.flagellin.

And that this nonsense about “Lyme And that this nonsense about “Lyme Disease” is nonsense.Disease” is nonsense.

Slide 37-- 1982, Alan Barbour on what Slide 37-- 1982, Alan Barbour on what happens when you treat Relapsing Fever happens when you treat Relapsing Fever

spirochetes with antibiotics: PMID#7103461 spirochetes with antibiotics: PMID#7103461

Slide 38Slide 38 Review Review We have seen what this Pam3Cys structure is, and we We have seen what this Pam3Cys structure is, and we

have seen that, to our ability to discover, it was first have seen that, to our ability to discover, it was first synthesized in 1983.synthesized in 1983.

We have seen that Brookhaven again tried to see what We have seen that Brookhaven again tried to see what was the actual structure of OspA (and the other Osps), was the actual structure of OspA (and the other Osps), but they could not do it without lopping off the lipids.but they could not do it without lopping off the lipids.

We have seen that there was some confusion over which We have seen that there was some confusion over which toll-like receptors were responsible for the immune toll-like receptors were responsible for the immune activation due to contamination, likely with activation due to contamination, likely with mycoplasma, but that Borrelial antigens are believed to mycoplasma, but that Borrelial antigens are believed to be handled by TLR2 and that Bb lacks LPS (managed by be handled by TLR2 and that Bb lacks LPS (managed by TLR4 and not 2)TLR4 and not 2)

Next we will look at Justin Radolf and Janis Weis and Next we will look at Justin Radolf and Janis Weis and why they think Bb OspA is Pam3Cys.why they think Bb OspA is Pam3Cys.

We are approaching We are approaching WHATWHAT could this OspA molecule could this OspA molecule be?, and be?, and HOWHOW does it act in the immune system? We do does it act in the immune system? We do not know why we also see it in the HIV vaccine. Why not know why we also see it in the HIV vaccine. Why people with HIV have antibodies to a synthetic Pam3Cys people with HIV have antibodies to a synthetic Pam3Cys remains unknown.remains unknown.

Reference 19 from the SchrReference 19 from the Schrööder reportder report= Justin Radolf, 1990, PMID: 2318538 = Justin Radolf, 1990, PMID: 2318538

Continuing Justin Radolf, 1990, PMID # Continuing Justin Radolf, 1990, PMID #

2318538 2318538 ““The OspA and OspB antigens were The OspA and OspB antigens were

radioimmunoprecipitated fromradioimmunoprecipitated from [[3H]palmitate-3H]palmitate-labeledlabeled detergent-phase proteins with detergent-phase proteins with monoclonalmonoclonal antibodies, and [3H]palmitate was antibodies, and [3H]palmitate was recovered unaltered from theserecovered unaltered from these proteins after proteins after sequential alkaline and acid hydrolyses. sequential alkaline and acid hydrolyses. TheThe

combined results provide formal combined results provide formal confirmation that the majorconfirmation that the major B. burgdorferi B. burgdorferi immunogens extracted by Triton X-114 immunogens extracted by Triton X-114 are lipoproteins.are lipoproteins. The demonstration that B. The demonstration that B. burgdorferi integral membrane antigensburgdorferi integral membrane antigens are are lipoproteins may explain…lipoproteins may explain…

Continuing Justin Radolf 1990, PMID # Continuing Justin Radolf 1990, PMID #

23185382318538

“…“…the basis of their immunogenicitythe basis of their immunogenicity

and may help to improve our and may help to improve our understanding of the surface topologyunderstanding of the surface topology

of B. burgdorferi. …”of B. burgdorferi. …”

(Structure is Function, or Function is (Structure is Function, or Function is Structure)Structure)

Slide 42 -- Radolf, et al, 1990, 2318538Slide 42 -- Radolf, et al, 1990, 2318538

Justin Radolf in 1990 extracted the Justin Radolf in 1990 extracted the lipids and was able to use heavy lipids and was able to use heavy hyrogen labeled H(3) palmitate to hyrogen labeled H(3) palmitate to determine that these lipids came on determine that these lipids came on and off the spirochete intact, lending and off the spirochete intact, lending his group to believe the lipoproteins his group to believe the lipoproteins were Pam3Cys- 3 acyl groups. were Pam3Cys- 3 acyl groups.

Slide 43- 1994, Janis Weis discovers that… [PubMed # 7927731]

Weis, 1994 Weis, 1994 “Native OspA is active a “Native OspA is active a concentrations lower than these synthetic concentrations lower than these synthetic lipopeptides…unique modifications by the lipopeptides…unique modifications by the

spirochete.” spirochete.”

She is saying that Bb may be taking up She is saying that Bb may be taking up the palmitic acid groups intact, but the palmitic acid groups intact, but

somehow the spirochete arranges the somehow the spirochete arranges the lipids so thatlipids so that they’re more toxic when the they’re more toxic when the

bugbug produces them.produces them.

Mini-ReviewMini-Review

We’re trying to find out what OspA is, We’re trying to find out what OspA is, since structure = function.since structure = function.In the late 1990s, we find that OspA is a In the late 1990s, we find that OspA is a Pam3Cys (Mario Philipp; PMID# 9864208).Pam3Cys (Mario Philipp; PMID# 9864208).

So, where does Pam3Cys come from?So, where does Pam3Cys come from?It’s a synthetic analog of It’s a synthetic analog of E. coliE. coli Lipid A and Lipid A and these scientists think it is also found in these scientists think it is also found in mycobacteria and spirochetes…mycobacteria and spirochetes…

By performing experiments to separate By performing experiments to separate the lipids (Radolf, growing bacteria in the lipids (Radolf, growing bacteria in radiolabeled palmitate), the scientists radiolabeled palmitate), the scientists think it is Pam3Cys. Weis is not sure… think it is Pam3Cys. Weis is not sure…

So, you may say to yourself, “Self, So, you may say to yourself, “Self, what could this MoFo be?”what could this MoFo be?”

Where is Pam3Cys on the HIV virus?Where is Pam3Cys on the HIV virus?

It’s pretty hard to tell because the It’s pretty hard to tell because the patenteers are rather stingy with patenteers are rather stingy with their patent graphics. From the their patent graphics. From the Koreans who blew this synthetic HIV Koreans who blew this synthetic HIV 120/41 up as shown previously, they 120/41 up as shown previously, they say these Pam3Cys structures are say these Pam3Cys structures are the envelop glycoproteins, but other the envelop glycoproteins, but other references show gp120 and gp41 references show gp120 and gp41 themselves are Pam3Cys.themselves are Pam3Cys.

If you go to Wikipedia and steal If you go to Wikipedia and steal the HIV image like we did…the HIV image like we did…

Are they sure???Are they sure???

Is this really both OspA and the HIV Is this really both OspA and the HIV glycoproteins?glycoproteins?

Remember, Lyme has lipoproteins Remember, Lyme has lipoproteins and HIV 120/41 are glycoproteins (but and HIV 120/41 are glycoproteins (but no glycolipids, like no glycolipids, like E. coliE. coli Lipid A), and Lipid A), and all the scientists seem pretty all the scientists seem pretty convinced that this Pam3Cys is what convinced that this Pam3Cys is what the antigen is…the antigen is…

Thinkin’ ?Thinkin’ ?[SUBROUTINE 2; Steere/RICO, slide 1][SUBROUTINE 2; Steere/RICO, slide 1]

Try to recall if Alan Barbour or Allen Try to recall if Alan Barbour or Allen Steere or Gary Wormser or Durland Steere or Gary Wormser or Durland Fish or Larry Zemel or Lenny Sigal… Fish or Larry Zemel or Lenny Sigal… have ever mentioned what OspA have ever mentioned what OspA is/does….is/does….

Slide 51Slide 51-- Allen Steere’s Baby -- Allen Steere’s Baby [SUBROUTINE 2; Steere/RICO, slide 2][SUBROUTINE 2; Steere/RICO, slide 2]

Originally, OspA was supposedly the thing against Originally, OspA was supposedly the thing against which persons with Lyme Arthritis had an allergic which persons with Lyme Arthritis had an allergic response (too many antibodies). response (too many antibodies). It is the usual practice to assay for the efficacy of a It is the usual practice to assay for the efficacy of a vaccine by vaccine by testing with antigens that are testing with antigens that are different from the vaccine antigens.different from the vaccine antigens.

Therefore, Allen Steere went to Germany in 1992 Therefore, Allen Steere went to Germany in 1992 alone with bogus “high-passage” strains and alone with bogus “high-passage” strains and recombinant OspA-B from US strain B31 (with no recombinant OspA-B from US strain B31 (with no very immunogenic lipids attached) to leave OspA-very immunogenic lipids attached) to leave OspA-B out of the current or Dearborn diagnostic B out of the current or Dearborn diagnostic standard. (See the PPT on Scientific Validity).standard. (See the PPT on Scientific Validity).

Who was Allen Steere working with when he Who was Allen Steere working with when he went to Europe to leave OspA-B out of the went to Europe to leave OspA-B out of the standard? standard? [SUBROUTINE 2; Steere/RICO, slide 3][SUBROUTINE 2; Steere/RICO, slide 3]

OspA was intended to be the vaccine because people with OspA was intended to be the vaccine because people with Lyme arthritis have high antibodies to OspA, and OspA was Lyme arthritis have high antibodies to OspA, and OspA was the most abundant surface antigen on these spirochetes the most abundant surface antigen on these spirochetes fresh out of a tick.fresh out of a tick.

So OspA-B were left out of the diagnostic standard via some So OspA-B were left out of the diagnostic standard via some Steere lab-and-strains shenanigans. In this way, whoever Steere lab-and-strains shenanigans. In this way, whoever develops a test that leaves OspA-B (they’re encoded on the develops a test that leaves OspA-B (they’re encoded on the same plasmid, so they’re hard to separate.; you have to same plasmid, so they’re hard to separate.; you have to take the plasmid out) will have a monopoly on all the take the plasmid out) will have a monopoly on all the national blood testing after LYMErix comes on the market…national blood testing after LYMErix comes on the market…

This all would have happened if there was no Blot-Smudging This all would have happened if there was no Blot-Smudging problem because you can’t test for vaccine efficacy with problem because you can’t test for vaccine efficacy with the same antigen as the vaccine.the same antigen as the vaccine.

Let’s take a look at that RICO patent and the Let’s take a look at that RICO patent and the researchers at Yale who “helped.” researchers at Yale who “helped.”

[SUBROUTINE 2; Steere/RICO, slide 4][SUBROUTINE 2; Steere/RICO, slide 4]

USPTO.gov Patent No. 6,045,804 USPTO.gov Patent No. 6,045,804

“The present invention provides a method useful to detect a B. burgdorferi infection in a subject. The method provided by the invention is particularly useful to discriminate B. burgdorferi infection from OspA vaccination, although it is sufficiently sensitive and specific to use in any general Lyme disease screening or diagnostic application. Thus, the method of the invention is particularly appropriate for large scale screening or diagnostic applications where only part of the subject population has been vaccinated or where the vaccination status of the population is unknown. “

Who is Helpin Dave Persing…? Who is Helpin Dave Persing…? (We will look at this again later.)(We will look at this again later.)[SUBROUTINE 2; Steere/RICO, slide 5, PMID [SUBROUTINE 2; Steere/RICO, slide 5, PMID

8968914]8968914]

In 1995 and 1996 the Yale Lyme criminals knew they had In 1995 and 1996 the Yale Lyme criminals knew they had problems reading Western Blots in LYMErix vaccinated people. problems reading Western Blots in LYMErix vaccinated people.

This is from the Schoen/Fikrig/Persing RICO Method report. This is from the Schoen/Fikrig/Persing RICO Method report.The point is that they never told the FDA they could not read The point is that they never told the FDA they could not read their Western Blots in OspA-vaccinated people, and that they their Western Blots in OspA-vaccinated people, and that they therefore had no way to tell whether or not OspA vaccination therefore had no way to tell whether or not OspA vaccination

prevented “Lyme Disease”prevented “Lyme Disease” [PubMed ID #: 8968914] [SUBROUTINE 2; Steere/RICO, slide [PubMed ID #: 8968914] [SUBROUTINE 2; Steere/RICO, slide

6]6]

Did they report these problems to the FDA Did they report these problems to the FDA when they told the FDA they had a vaccine when they told the FDA they had a vaccine

in 1998?in 1998? [SUBROUTINE 2; Steere/RICO, slide 7][SUBROUTINE 2; Steere/RICO, slide 7]

No.No.

““We focused on reports of arthritis and facial We focused on reports of arthritis and facial paralysis because these have been associated with paralysis because these have been associated with

Lyme disease”Lyme disease”

Let’s go back and see if Pam3Cys Let’s go back and see if Pam3Cys really is on the Syphilis spirochete, too. really is on the Syphilis spirochete, too.

Lipopolysaccharide Binding Protein Binds Lipopolysaccharide Binding Protein Binds to Triacylated and Diacylated Lipopeptides to Triacylated and Diacylated Lipopeptides and Mediates Innate Immune Responsesand Mediates Innate Immune Responses11

2004: Schroder, PMID #15294986 2004: Schroder, PMID #15294986

“Because the N-terminal Pam3Cys modification is

essential for immunoactivation caused by lipoproteins of B. burgdorferi as well as of another spirochete, Treponema pallidum (21), subsequent studies investigating immune responses to spirochetes used synthetic lipopeptides (22).”

Let’s look at those referencesLet’s look at those references

““Because the N-terminal PamBecause the N-terminal Pam33Cys Cys

modification is essentialmodification is essential for for immunoactivation caused by immunoactivation caused by lipoproteins of B. burgdorferilipoproteins of B. burgdorferi as well as well as of another spirochete, Treponema as of another spirochete, Treponema pallidum (pallidum (2121), subsequent), subsequent studies studies investigating immune responses to investigating immune responses to spirochetes used syntheticspirochetes used synthetic

lipopeptides (lipopeptides (2222). ).

The references are 1994 & 1995, Radolf and The references are 1994 & 1995, Radolf and

Weis, again PWeis, again PMID # 7927731MID # 7927731

PMID 7927731, Weis, 1994 PMID 7927731, Weis, 1994 AbstractAbstract

““Borrelia burgdorferi lipoproteins are Borrelia burgdorferi lipoproteins are 50- to 500-fold50- to 500-fold more more activeactive as cytokine inducers and B-cell mitogens than as cytokine inducers and B-cell mitogens than Escherichia coli lipoproteins Escherichia coli lipoproteins and synthetic peptides and synthetic peptides containing the tripalmitoyl-S-glyceryl-cysteine moiety.containing the tripalmitoyl-S-glyceryl-cysteine moiety. To investigate the source of this unique potency, we To investigate the source of this unique potency, we compared native OspA from B. burgdorferi with compared native OspA from B. burgdorferi with recombinant lipidated OspA produced in E. coli.recombinant lipidated OspA produced in E. coli. As little As little as 10 ng of either protein per ml stimulated B-cell as 10 ng of either protein per ml stimulated B-cell proliferation and production of cytokines and nitric oxide by proliferation and production of cytokines and nitric oxide by macrophages. The two proteins induced comparable antibody macrophages. The two proteins induced comparable antibody responses in mice. responses in mice. Nonlipidated OspA made in E. coli had Nonlipidated OspA made in E. coli had no stimulatory activity.no stimulatory activity. Thus, lipid modification is essential Thus, lipid modification is essential both in vivo and in vitro for the immunological properties of both in vivo and in vitro for the immunological properties of OspA. The lipid moiety appears equally active whether OspA. The lipid moiety appears equally active whether produced in B. burgdorferi or in E. coli.”produced in B. burgdorferi or in E. coli.”

These researchers keep cross-These researchers keep cross-referencing their own reportsreferencing their own reports

But are we really sure OspA and the HIV But are we really sure OspA and the HIV gps are really Pam3Cys??gps are really Pam3Cys??

What ways are there to tell, since you can’t What ways are there to tell, since you can’t recrystallize a lipid? So these researchers recrystallize a lipid? So these researchers simply do comparative immune response simply do comparative immune response studies. The best we, the outside studies. The best we, the outside observers, can guess is that these observers, can guess is that these scientists really think these antigens are scientists really think these antigens are Pam3Cys.Pam3Cys.

Now recall that way, way back a Now recall that way, way back a hundred years ago in 2004…hundred years ago in 2004…

This thing, whatever it is, is also This thing, whatever it is, is also found in mycoplasma and found in mycoplasma and mycobacteria.mycobacteria.

Okay, so how do we know Okay, so how do we know THATTHAT for for sure?sure?

Aha! The famous Toll-Like-Aha! The famous Toll-Like-Receptors…Receptors…PMID: 10426995PMID: 10426995

Continuing with the text of the Continuing with the text of the previous TLR report…previous TLR report…

““The 19-kD M. tuberculosis lipoprotein is a The 19-kD M. tuberculosis lipoprotein is a member of a family of prokaryotic lipoproteins. member of a family of prokaryotic lipoproteins. Lipoproteins have been found extensivelyLipoproteins have been found extensively in in both Gram-positive and Gram-negative both Gram-positive and Gram-negative bacteria, including Treponemabacteria, including Treponema pallidum, pallidum, Mycoplasma species, and Borrelia burgdorferi (Mycoplasma species, and Borrelia burgdorferi (22-2422-24).). Profound immunoregulatory functions Profound immunoregulatory functions have been attributed to lipoproteins,have been attributed to lipoproteins, including including monocyte or macrophage activation (monocyte or macrophage activation (2525). ). TheThe

portion of lipoprotein responsible for its portion of lipoprotein responsible for its immunologic activityimmunologic activity is located in the NHis located in the NH22--terminal triacylated lipopeptide region.terminal triacylated lipopeptide region.

Removal ofRemoval of

Continuing the TLR reportContinuing the TLR report ““Removal of this lipid element Removal of this lipid element rendered the rendered the

parent product nonactivating,parent product nonactivating, and and synthetic lipopeptides could activate B cells synthetic lipopeptides could activate B cells and macrophagesand macrophages ((2323, , 2424, , 2626). Studies of the). Studies of the

B. burgdorferi OspA lipoprotein and the 47-kD B. burgdorferi OspA lipoprotein and the 47-kD lipopeptide of T.lipopeptide of T. pallidum demonstrated pallidum demonstrated lipoprotein induction of IL-12 mRNA (lipoprotein induction of IL-12 mRNA (2424,, 2727). ). We found that OspA and the NHWe found that OspA and the NH22-terminal -terminal lipopeptidelipopeptide of the T. pallidum 47-kD antigen of the T. pallidum 47-kD antigen activated IL-12 p40 promoteractivated IL-12 p40 promoter activity by a TLR-activity by a TLR-dependent mechanism (dependent mechanism (Fig. 2Fig. 2C), therebyC), thereby

providing evidence that TLRs serve to providing evidence that TLRs serve to recognize a diverse familyrecognize a diverse family of microbial of microbial lipoproteins. lipoproteins.

Continuing the TLR report…Continuing the TLR report…

““A monoclonal antibody specific to humanA monoclonal antibody specific to human TLR-TLR-2 (2 (2828) blocked the ability of LPS and the 19-kD) blocked the ability of LPS and the 19-kD

lipoprotein to stimulate IL-12 production from lipoprotein to stimulate IL-12 production from primary human monocytes,primary human monocytes, indicating the indicating the crucial role for TLR-2 in monocyte activation crucial role for TLR-2 in monocyte activation byby these microbial molecules (these microbial molecules (Fig. 2Fig. 2D). D). Because the deacylatedBecause the deacylated OspA (d-OspA) OspA (d-OspA) was unable to activate IL-12 production was unable to activate IL-12 production from THP-1from THP-1 cells (cells (2929), the fatty acyl ), the fatty acyl moiety, which is geneticallymoiety, which is genetically and and structurally conserved among microbial structurally conserved among microbial lipoproteins, appearslipoproteins, appears to be crucial for to be crucial for monocyte activation through TLRs.monocyte activation through TLRs.

You with that?You with that?

““Removal of this lipid element rendered Removal of this lipid element rendered the parent product nonactivating,the parent product nonactivating, and and synthetic lipopeptides could activate B synthetic lipopeptides could activate B cells and macrophagescells and macrophages ((2323, , 2424, , 2626). ).

Don’t remove the lipids or else you’re not Don’t remove the lipids or else you’re not going to get as serious an antibody going to get as serious an antibody response, yet that’s just what Allen Steere response, yet that’s just what Allen Steere did with his Strains Tricks In Europe to did with his Strains Tricks In Europe to leave OspA and B out of the standard we leave OspA and B out of the standard we now have.now have.

Slide 69 -- Mechanisms and evidence of immune Slide 69 -- Mechanisms and evidence of immune suppression associated with spirochetal and suppression associated with spirochetal and mycoplasmal antigens … And the New Great Imitatorsmycoplasmal antigens … And the New Great Imitators

1) Justin Radolf on HLA downregulation [11441098] 1) Justin Radolf on HLA downregulation [11441098] [slide 70][slide 70]2) Ablation of IRAK-associated kinase [15294992] 2) Ablation of IRAK-associated kinase [15294992] 3) Inhibition of TNF-alpha induced apoptosis [15286682] (see also 3) Inhibition of TNF-alpha induced apoptosis [15286682] (see also

Duray)Duray)4) Inhibition of NF-kappa [12496438]4) Inhibition of NF-kappa [12496438]Alan Barbour on blebbing as autovaccination with Osps Alan Barbour on blebbing as autovaccination with Osps [slide 73][slide 73]Tuberculosis Lipoprotein Vaccine Failures (3) Tuberculosis Lipoprotein Vaccine Failures (3) [slide 75] [slide 75] Gary Wormser on OspA-induced immunosuppression [10865170] Gary Wormser on OspA-induced immunosuppression [10865170] [slide [slide

78]78]McSweegan Attacks McSweegan Attacks [slide 79][slide 79]Paul Duray and EBV-immortalized cells; MS (1989 and 1992) Paul Duray and EBV-immortalized cells; MS (1989 and 1992) [slide 86][slide 86]Joseph G. Tully and mycoplasma in cancer and effect on erythrocytes Joseph G. Tully and mycoplasma in cancer and effect on erythrocytes

(Chronic Fatigue)(Chronic Fatigue)Mycoplasma attaching to RBCs (Chronic Fatigue)Mycoplasma attaching to RBCs (Chronic Fatigue)Halperin on Lyme and ALSHalperin on Lyme and ALSALS and fungal antigensALS and fungal antigensGulf War Illness and ALS (and Sam T. Donta and Mycoplasma and GWI)Gulf War Illness and ALS (and Sam T. Donta and Mycoplasma and GWI)The Murdered Iraqi/ Plum Island Mycoplasmal BioweaponeerThe Murdered Iraqi/ Plum Island Mycoplasmal Bioweaponeer

Re-enter the very excellent scientist, Justin Re-enter the very excellent scientist, Justin Radolf, 2001, PubMed ID # 11441098 Radolf, 2001, PubMed ID # 11441098

(Radolf is a jerk, but he’s a good scientist.)(Radolf is a jerk, but he’s a good scientist.)

So, Justin Radolf comes along and So, Justin Radolf comes along and says…says…

Oh, yeah. Like the failed tuberculosis Oh, yeah. Like the failed tuberculosis lipoprotein vaccines – that made the lipoprotein vaccines – that made the infections worse, just like LYMErix – we infections worse, just like LYMErix – we find that these lipoproteins cause the find that these lipoproteins cause the cells to stop presenting antigen (making cells to stop presenting antigen (making antibodies)… And if Lyme/LYMErix antibodies)… And if Lyme/LYMErix victims are not making antibodies, they victims are not making antibodies, they are very Munchausery, self-poisoners, are very Munchausery, self-poisoners, “seronegative,” or are “women and “seronegative,” or are “women and girls.”girls.”

No. That’s not what Radolf said. No. That’s not what Radolf said. He said: He said:

““Inhibition of MHC-II AgInhibition of MHC-II Ag processing by either processing by either MTB bacilli or purified MTB 19-kDa lipoproteinMTB bacilli or purified MTB 19-kDa lipoprotein

was dependent on Toll-like receptor (TLR) 2 was dependent on Toll-like receptor (TLR) 2 and independentand independent of TLR 4. Synthetic analogs of TLR 4. Synthetic analogs of lipopeptides from Treponema pallidumof lipopeptides from Treponema pallidum also also inhibited Ag processing. Despite the ability of inhibited Ag processing. Despite the ability of MTB 19-kDaMTB 19-kDa lipoprotein to activate lipoprotein to activate microbicidal and innate immune functionsmicrobicidal and innate immune functions

early in infection, TLR 2-dependent inhibition early in infection, TLR 2-dependent inhibition of MHC-II expressionof MHC-II expression and Ag processing by and Ag processing by MTB 19-kDa lipoprotein during later phasesMTB 19-kDa lipoprotein during later phases of of macrophage infection may macrophage infection may prevent prevent presentation of MTB Agspresentation of MTB Ags and decrease and decrease recognition by T cells. recognition by T cells.

And he says…And he says…

This mechanism may allowThis mechanism may allow intracellular intracellular MTB to evade immune surveillance and MTB to evade immune surveillance and maintainmaintain chronic infection.chronic infection.

So, Justin Radolf says, essentially:So, Justin Radolf says, essentially: Vaccination with Vaccination with OspA will render you unable to make OspA will render you unable to make antibodies against Lyme and antibodies against Lyme and mycoplasma and mycobacteria. So stop mycoplasma and mycobacteria. So stop poisoning yourselves with OspA poisoning yourselves with OspA vaccination.vaccination.

One more thing you need to know about One more thing you need to know about Techniques of Self-Poisoning (by Alan Techniques of Self-Poisoning (by Alan

Barbour)Barbour)

“Many researchers believe that the secret to B. burgdorferi's infectivity and inflammatory capacity lies in the interaction of its surface proteins with the host's immunological system Yale researcher Stephen Barthold, a veterinarian and professor of comparative medicine who developed the first mouse model of Lyme disease, studies the expression of B. burgdorferi surface proteins throughout various stages of the spirochete’s life cycle. He finds that during the early stages of infection, B. burgdorferi avoids immune detection by decreasing its expression of surface proteins or cloaking its expressed surface proteins under a layer of slime…

Continued (Alan Barbour on Continued (Alan Barbour on Spirochetal blebbing, Spirochetal blebbing, The ScientistThe Scientist

1996, 1996, 1010(14)(14)::13 ):13 ): …. "It's using some sort of stealth-bomber-type

mechanism," he says. Or, using another diversionary tactic called blebbing, the spirochete can pinch off bits of its membrane in order to release its surface proteins. Explains Barbour: “It’s like a bacterial Star Wars defense program" in which released surface proteins might intercept incoming host antibodies, keeping the spirochete safe from immunological attack. “

This of course is the auto-vaccination with the immune This of course is the auto-vaccination with the immune suppressing OspA-like antigens, which render you suppressing OspA-like antigens, which render you seronegative after a time. seronegative after a time.

It’s stealth bombery and Munchausery and the vaccine. It’s stealth bombery and Munchausery and the vaccine. You too can be a shienticks!You too can be a shienticks!

Tuberculosis Lipoprotein Tuberculosis Lipoprotein Vaccines FailuresVaccines Failures

10792376, 10792376, 2000 Blackwell Science Ltd2000 Blackwell Science Ltd

The 19-kD antigen and protective immunity The 19-kD antigen and protective immunity in a murine model of tuberculosis:in a murine model of tuberculosis:

““These results are consistent with a model These results are consistent with a model in which the presence of the 19-kD protein in which the presence of the 19-kD protein has a detrimental effect on the efficacy of has a detrimental effect on the efficacy of vaccination with live mycobacteria. “vaccination with live mycobacteria. “

PubMed ID 11179309PubMed ID 11179309 Infect Immun. 2001 Mar;69(3):1433-9.Infect Immun. 2001 Mar;69(3):1433-9.

Mycobacterium tuberculosis 19-kilodalton lipoprotein Mycobacterium tuberculosis 19-kilodalton lipoprotein inhibits Mycobacterium smegmatis-induced cytokine inhibits Mycobacterium smegmatis-induced cytokine production by human macrophages in vitro.production by human macrophages in vitro.

““Thus, the immunosuppressive effect is dependent Thus, the immunosuppressive effect is dependent on glycosylated and acylated 19-kDa lipoprotein on glycosylated and acylated 19-kDa lipoprotein present in the phagosome containing the present in the phagosome containing the mycobacterium. These results suggest that the mycobacterium. These results suggest that the diminished protection against challenge with M. diminished protection against challenge with M. tuberculosis seen in mice vaccinated with M. tuberculosis seen in mice vaccinated with M. smegmatis expressing the 19-kDa lipoprotein is the smegmatis expressing the 19-kDa lipoprotein is the result of reduced TNF-alpha and IL-12 production, result of reduced TNF-alpha and IL-12 production, possibly leading to reduced induction of T-cell possibly leading to reduced induction of T-cell activation.”activation.”

Slide 78Slide 78 PubMed ID # 12761093PubMed ID # 12761093 Infect Immun. 2003 Jun;71(6):3146-54.Infect Immun. 2003 Jun;71(6):3146-54.

The Mycobacterium tuberculosis Recombinant 27-The Mycobacterium tuberculosis Recombinant 27-Kilodalton Lipoprotein Induces a Strong Th1-Type Kilodalton Lipoprotein Induces a Strong Th1-Type Immune Response Deleterious to Protection Immune Response Deleterious to Protection

““Surprisingly, in 27-kDa-vaccinated mice (protein or Surprisingly, in 27-kDa-vaccinated mice (protein or DNA vaccines) challenged by M. tuberculosis H37Rv DNA vaccines) challenged by M. tuberculosis H37Rv or BCG strains, there was a significant increase in the or BCG strains, there was a significant increase in the numbers of CFU in the spleen compared to that for numbers of CFU in the spleen compared to that for control groups. control groups. Furthermore, the protection Furthermore, the protection provided by BCG or other mycobacterial provided by BCG or other mycobacterial antigens was completely abolished once the 27-antigens was completely abolished once the 27-kDa antigen was added to the vaccine kDa antigen was added to the vaccine preparations.preparations. This study indicates that the 27-kDa This study indicates that the 27-kDa antigen has an adverse effect on the protection antigen has an adverse effect on the protection afforded by recognized vaccines. We are currently afforded by recognized vaccines. We are currently studying how the 27-kDa antigen modulates the studying how the 27-kDa antigen modulates the mouse immune response.”mouse immune response.”

Gary Wormser reporting that OspA Gary Wormser reporting that OspA suppresses the immune response in suppresses the immune response in

2000 2000 PMID# 10865170 PMID# 10865170

Modulation of lymphocyte proliferative responses by a Modulation of lymphocyte proliferative responses by a canine Lyme disease vaccine of recombinant outer canine Lyme disease vaccine of recombinant outer surface protein A (OspA).surface protein A (OspA).

““After exposure to either the unaltered vaccine After exposure to either the unaltered vaccine preparation or OspA prepared in salinepreparation or OspA prepared in saline, , normal normal lymphocyte responseslymphocyte responses to the mitogens concanavalin A, to the mitogens concanavalin A, phytohemagglutinin-M or pokeweed mitogen, or the antigen BCG phytohemagglutinin-M or pokeweed mitogen, or the antigen BCG were consistently reducedwere consistently reduced. Whole cell extracts of B. . Whole cell extracts of B. burgdorferi also modulated immune responses but required a burgdorferi also modulated immune responses but required a much greater quantity of protein than needed for the OspA much greater quantity of protein than needed for the OspA preparation. preparation. The magnitude of modulation was directly The magnitude of modulation was directly dependent on the quantity of OspA.dependent on the quantity of OspA. OspA interferes with the OspA interferes with the response of lymphocytes to proliferative stimuli response of lymphocytes to proliferative stimuli including a including a blocking of cell cycle phase progression.blocking of cell cycle phase progression. Future studies Future studies designed to delete the particular region or component of the designed to delete the particular region or component of the OspA molecule responsible for this effect may lead to improved OspA molecule responsible for this effect may lead to improved vaccine preparations. vaccine preparations.

Now don’t forget, Lyme victims came Now don’t forget, Lyme victims came under vicious attacks by the under vicious attacks by the

McSweegan gang online at that timeMcSweegan gang online at that time

On the newsgroup and elsewhere, anyone who said On the newsgroup and elsewhere, anyone who said anything about Lyme or the vaccines was stalked anything about Lyme or the vaccines was stalked and harassed by the likes of Durland Fish and and harassed by the likes of Durland Fish and Edward McSweegan. This can be verified in the Edward McSweegan. This can be verified in the stalking and harassment lawsuits in addition to the stalking and harassment lawsuits in addition to the fact that McSweegan was present at the January 31, fact that McSweegan was present at the January 31, 2001 FDA Vaccine Meeting on LYMErix, and a few 2001 FDA Vaccine Meeting on LYMErix, and a few days later claimed the presentation demonstrating days later claimed the presentation demonstrating the EXACT PROBLEM with Lyme and LYMErix the EXACT PROBLEM with Lyme and LYMErix demonstrated that we Lyme scientists were crazy.demonstrated that we Lyme scientists were crazy.

Anonymous internet harassment is now a federal Anonymous internet harassment is now a federal crime.crime.

  MCSWEEGAN: MCSWEEGAN: “I think the only thing it accomplished was to “I think the only thing it accomplished was to remind the audience that 1) easy access to scientific and medical remind the audience that 1) easy access to scientific and medical

information is no substitute for real knowledge and 2) ‘some of information is no substitute for real knowledge and 2) ‘some of these people are nuts.’"these people are nuts.’"

Says Kathleen Dickson to the Jan 31, 2001 FDA Says Kathleen Dickson to the Jan 31, 2001 FDA Vaccine Committee: Vaccine Committee: “THE PROBLEM IS THE “THE PROBLEM IS THE

DEARBORN/DRESSLER IgG STANDARDDEARBORN/DRESSLER IgG STANDARD””

Kathleen Dickson Kathleen Dickson alsoalso said to the FDA said to the FDA

Vaccine Committee (LYMErix):Vaccine Committee (LYMErix):

Slide 84Slide 84 -- The main issue for these Lyme -- The main issue for these Lyme criminals is that they knew they had no way criminals is that they knew they had no way to test whether they actually had vaccines or to test whether they actually had vaccines or

not.not.

They They allall said they could not read their Western Blots in LYMErix said they could not read their Western Blots in LYMErix or ImmuLyme (CDC officer Alan Barbour’s OspA patent) or or ImmuLyme (CDC officer Alan Barbour’s OspA patent) or OspA, and at Dearborn, no one agreed with Allen Steere OspA, and at Dearborn, no one agreed with Allen Steere regarding his “5 of 10 bands” Dressler/Steere proposal (so it regarding his “5 of 10 bands” Dressler/Steere proposal (so it wasn’t even a “consensus” conference). Steere’s Dearborn wasn’t even a “consensus” conference). Steere’s Dearborn criteria was only accurate to the tune of 15-20% or missed criteria was only accurate to the tune of 15-20% or missed 85-80% of the cases, per the Dearborn submissions.85-80% of the cases, per the Dearborn submissions.

OspA Adverse Events cases, these simply blew off, lied about it OspA Adverse Events cases, these simply blew off, lied about it to the FDA, lied about it in the journals, and to the public.to the FDA, lied about it in the journals, and to the public.

WHYWHY did they lie and why do they lie, still? did they lie and why do they lie, still? They’re scared to death of criminal charges because these are They’re scared to death of criminal charges because these are

murder charges.murder charges.

And that’s the reason for all the stalking and harassment by the And that’s the reason for all the stalking and harassment by the likes of NIH’s Edward McSweegan and Yale’s Durland Fish.likes of NIH’s Edward McSweegan and Yale’s Durland Fish.

NIH’s Edward McSweegan said we NIH’s Edward McSweegan said we were “nuts” to be saying OspA wasn’t were “nuts” to be saying OspA wasn’t

a vaccine after Gary Wormser reported a vaccine after Gary Wormser reported that OspA could not be a vaccine.that OspA could not be a vaccine.

““Actually, it wasn't great.  It was a rambling, buzzsaw Actually, it wasn't great.  It was a rambling, buzzsaw critique of the Dressler serodiagnostic criteria, with critique of the Dressler serodiagnostic criteria, with washed out, illegible black-and-white overheads.   washed out, illegible black-and-white overheads.  

““Worse, it had nothing to do with the topic of Worse, it had nothing to do with the topic of meeting which was a "Lyme Disease Vaccine Safety meeting which was a "Lyme Disease Vaccine Safety Update."  I think the only thing it accomplished was Update."  I think the only thing it accomplished was to remind the audience that 1) easy access to to remind the audience that 1) easy access to scientific and medical information is no substitute for scientific and medical information is no substitute for real knowledge and 2) "some of these people are real knowledge and 2) "some of these people are nuts."nuts."

But a person could always be an analytical But a person could always be an analytical methods development chemist and do this methods development chemist and do this kind VALIDATION OF ANALYTICAL METHODS kind VALIDATION OF ANALYTICAL METHODS

of work for a living, say, at Pfizer.of work for a living, say, at Pfizer.

Not only is it very well-known that McSweegan Not only is it very well-known that McSweegan does “no work” for a living, he would not does “no work” for a living, he would not know the first thing about analytical know the first thing about analytical methods validations, not being a chemist. methods validations, not being a chemist.

While he was at the FDA (LYMErix Vaccine While he was at the FDA (LYMErix Vaccine Meeting in Jan, 2001) he could have asked Meeting in Jan, 2001) he could have asked the FDA about how it was done.the FDA about how it was done.

No-Work McSweeganNo-Work McSweegan

What else does Pam3Cys OspA What else does Pam3Cys OspA do?do?

Okay, we know that Pam3Cys is managed by Okay, we know that Pam3Cys is managed by TLR2, and according to some people, also TLR2, and according to some people, also TLR1 or TLR6. This research continues to be TLR1 or TLR6. This research continues to be refined.refined.

And we know it results in the downregulation And we know it results in the downregulation of HLA or inhibits antibody production. Radolf, of HLA or inhibits antibody production. Radolf, PMID# 11441098 PMID# 11441098

But how, exactly, does this lipid screw up the But how, exactly, does this lipid screw up the normal process of antigen presentation normal process of antigen presentation through the HLAs? What other research is out through the HLAs? What other research is out there about what OspA/Pam3Cys does to the there about what OspA/Pam3Cys does to the immune system?immune system?

Enormously important research was un-Enormously important research was un-discovered as a result of Yale’s and Allen discovered as a result of Yale’s and Allen

Steere’s OspA scam…Steere’s OspA scam…

…….We were studying all the known mechanisms of .We were studying all the known mechanisms of illness associated with this disease because we real illness associated with this disease because we real Lyme victims know we’re not fooling around. And Lyme victims know we’re not fooling around. And we were hearing from LYMErix victims that they had we were hearing from LYMErix victims that they had a disease like Lyme (since they “had had Lyme a disease like Lyme (since they “had had Lyme before”). And we were studying about other effects before”). And we were studying about other effects of lipoproteins and errant lipoproteins, and the of lipoproteins and errant lipoproteins, and the macrophage activity and the toxicities associated macrophage activity and the toxicities associated with the free-radical degradation products with the free-radical degradation products (conveniently not mentioned by the Lyme crooks as (conveniently not mentioned by the Lyme crooks as regards OspA vaccination),… and it being recognized regards OspA vaccination),… and it being recognized that mycobacteria and mycoplasma have the same that mycobacteria and mycoplasma have the same type of antigens as Borrelia… and we stumbled upon type of antigens as Borrelia… and we stumbled upon mechanisms of inhibition of the auto-kill kinases by mechanisms of inhibition of the auto-kill kinases by some of these bizarre lipids and VIOLA!some of these bizarre lipids and VIOLA!

There There was Paul Duraywas Paul Duray.. In one of those famous epiphanous, serendipidous, In one of those famous epiphanous, serendipidous,

singularity moments where one makes that EUREKA!! singularity moments where one makes that EUREKA!! connection (which are more likely, “I forgot where I read it”)connection (which are more likely, “I forgot where I read it”)……

I forgot what was the clue. I think I was looking at toxic degradation I forgot what was the clue. I think I was looking at toxic degradation products of lipids degraded by the superoxides and quinolinic acid products of lipids degraded by the superoxides and quinolinic acid

and also the anti-apoptotic qualities of BCL2 promoters, quite and also the anti-apoptotic qualities of BCL2 promoters, quite honestly… Of course anyone who thinks a scientist should be a left-honestly… Of course anyone who thinks a scientist should be a left-

brainer, linear-thinker don’t know what it’s like to be a real brainer, linear-thinker don’t know what it’s like to be a real scientist, cuz we’re all VISUAL-SPATIAL. scientist, cuz we’re all VISUAL-SPATIAL.

How could it be otherwise? How could it be otherwise?

““These look like Epstein-Barr These look like Epstein-Barr transformed (immortalized) cells”transformed (immortalized) cells”

Duray, 1992, Cold Spring Harbor Duray, 1992, Cold Spring Harbor Conference, Transcribed:Conference, Transcribed:

"On occasion, these atypical-appearing large "On occasion, these atypical-appearing large lymphocytes have been misinterpreted in lymphocytes have been misinterpreted in biopsy by several laboratories as cells of a biopsy by several laboratories as cells of a malignant lymphoma or leukemia.  Bb malignant lymphoma or leukemia.  Bb antigens, then, may stimulate growth of antigens, then, may stimulate growth of immature lymphocytic suibsets in some target immature lymphocytic suibsets in some target organs, as well as in the cerebrospinal fluid organs, as well as in the cerebrospinal fluid (Szyfelbein and Ross 1988).  Usual bacterial (Szyfelbein and Ross 1988).  Usual bacterial infections do not produce such lymphocytic infections do not produce such lymphocytic infiltrates in tissue.  infiltrates in tissue.  These immunoblastoid These immunoblastoid cells in Bb infections at times resemble cells in Bb infections at times resemble those found in Epstein-Barr virus those found in Epstein-Barr virus infections.  Does Bb reactivate…infections.  Does Bb reactivate…

“…“…Does Bb reactivate latent Does Bb reactivate latent virus infections in tissues?virus infections in tissues?

“…“…Does Bb reactivate latent virus Does Bb reactivate latent virus infections in tissues? infections in tissues?  Do some tick inocula Do some tick inocula harbor simultaneous infectious agents (ixodid harbor simultaneous infectious agents (ixodid ticks can harbor Rickettsiae, Babesia microti, ticks can harbor Rickettsiae, Babesia microti, and Ehrlichia bacteria, in addition to Bb), and Ehrlichia bacteria, in addition to Bb), producing multi-agent infections in some producing multi-agent infections in some hosts?  Further studies can clarify these issues hosts?  Further studies can clarify these issues by means of tissue-based molecular probe by means of tissue-based molecular probe analysisanalysis." - ." - 

  Paul DurayPaul Duray, NCI, NIH, Ft. Detrick, at the 1992 , NCI, NIH, Ft. Detrick, at the 1992 Cold Spring Harbor Lyme Crooks' ConferenceCold Spring Harbor Lyme Crooks' Conference, published in Steve , published in Steve Schutzer's Schutzer's Lyme Disease: Molecular and Immunologic Lyme Disease: Molecular and Immunologic Approaches.Approaches.

Pretty much, LYMErix looked like it somehow Pretty much, LYMErix looked like it somehow reactivated Lyme, since that’s what the reactivated Lyme, since that’s what the

LYMErix victims told us on the phoneLYMErix victims told us on the phone

And Paul Duray was saying something And Paul Duray was saying something similar. And there was published data on similar. And there was published data on OspA inducing Interleukin-10, a cytokine that OspA inducing Interleukin-10, a cytokine that tends to tame or reduce the immune tends to tame or reduce the immune response (Mario Philipp)… It’s pretty hard to response (Mario Philipp)… It’s pretty hard to argue that OspA was a vaccine, when all the argue that OspA was a vaccine, when all the science and all the empirical evidence and science and all the empirical evidence and the bogus Dearborn “science” seemed to the bogus Dearborn “science” seemed to show that there was a real problem here, in show that there was a real problem here, in addition to the bizarre situation of being addition to the bizarre situation of being stalked and harassed by the likes of Edward stalked and harassed by the likes of Edward McSweegan and Yale’s Durland Fish.McSweegan and Yale’s Durland Fish.

Tolerance Induced by the Lipopeptide Tolerance Induced by the Lipopeptide PamPam33Cys Is Due to Ablation of IL-1R-Cys Is Due to Ablation of IL-1R-

Associated Kinase-1Associated Kinase-111

[PMID# 15294992, 2004][PMID# 15294992, 2004] ““PreculturePreculture of the cells with Pamof the cells with Pam33Cys at 1 µg/ml Cys at 1 µg/ml leads leads

to a reducedto a reduced response after subsequent response after subsequent stimulation with Pamstimulation with Pam33CysCys at 10 µg/ml, at 10 µg/ml, indicating indicating that the cells have become tolerant to Pamthat the cells have become tolerant to Pam33Cys. …Cys. …

““LPS acts via CD14 and the associated TLR4 (LPS acts via CD14 and the associated TLR4 (1919, , 2020, , 2121). In the). In the

present study, we have investigated which mechanisms present study, we have investigated which mechanisms operateoperate in tolerance induced via TLR2 by the synthetic in tolerance induced via TLR2 by the synthetic lipopeptide Pamlipopeptide Pam33Cys.Cys. Using this pathway of monocyte Using this pathway of monocyte activation, an entirely differentactivation, an entirely different mechanism appears to operate, mechanism appears to operate, because mobilization of NF- B-p50p65because mobilization of NF- B-p50p65 heterodimers is heterodimers is completely blocked. completely blocked. The failure to mobilizeThe failure to mobilize classical NF- B-classical NF- B-p50p65 is shown to be due to a blockade at thep50p65 is shown to be due to a blockade at the level of level of IRAK-1 protein in PamIRAK-1 protein in Pam33Cys-tolerant Mono Mac 6 cells.Cys-tolerant Mono Mac 6 cells. We We also confirm that LPS tolerance operates via induction ofalso confirm that LPS tolerance operates via induction of NF- B-NF- B-p50p50 homodimers and that highly purified (lipoprotein-free)p50p50 homodimers and that highly purified (lipoprotein-free)

LPS only partially inhibits IRAK-1 protein expression in LPS-LPS only partially inhibits IRAK-1 protein expression in LPS-toleranttolerant cells.cells.

Tolerance Induced by the Lipopeptide Tolerance Induced by the Lipopeptide PamPam33Cys Is Due to Ablation of IL-1R-Cys Is Due to Ablation of IL-1R-

Associated Kinase-1Associated Kinase-111

[PMID#15294992, 2004][PMID#15294992, 2004]

““Hence, in monocytes, different mechanisms of tolerance Hence, in monocytes, different mechanisms of tolerance appearappear to coexist. This indicates that it is important for the to coexist. This indicates that it is important for the hosthost to provide more than one mechanism to ensure down-to provide more than one mechanism to ensure down-regulationregulation of TNF and to prevent the detrimental effects of of TNF and to prevent the detrimental effects of excessive amountsexcessive amounts of proinflammatory cytokines during of proinflammatory cytokines during bacterial infection.bacterial infection.

““It is concluded that in Mono Mac 6 monocytic cells, It is concluded that in Mono Mac 6 monocytic cells, inhibition of IRAK-1 expression at the mRNA and inhibition of IRAK-1 expression at the mRNA and protein levels is the main TLR-2-dependent protein levels is the main TLR-2-dependent mechanism responsible for Pam(3)Cys-induced mechanism responsible for Pam(3)Cys-induced tolerance, but not for TLR-4-dependent LPS-induced tolerance, but not for TLR-4-dependent LPS-induced tolerance.”tolerance.”

Review of where Pam3Cys is found (previous Review of where Pam3Cys is found (previous slides, slide 28, PMID 15294986, 2004)slides, slide 28, PMID 15294986, 2004)

“…“…subsequentsubsequent studies investigating immune studies investigating immune responses to spirochetes used syntheticresponses to spirochetes used synthetic lipopeptides lipopeptides ((2222). The ). The PamPam33CysCys moiety was also reported to moiety was also reported to

bebe present in cytokine-inducing lipoproteins of present in cytokine-inducing lipoproteins of Mycobacterium andMycobacterium and Mycoplasma spp. (Mycoplasma spp. (2323, , 2424);); thus, it can be regarded as a highlythus, it can be regarded as a highly conserved conserved molecular motif among different classes of bacteria.molecular motif among different classes of bacteria.

In Mycoplasma fermentans, the presence of a In Mycoplasma fermentans, the presence of a macrophage stimulatingmacrophage stimulating lipopeptide, termed 2-kDa lipopeptide, termed 2-kDa macrophage-activating lipopeptidemacrophage-activating lipopeptide ((MALP-2MALP-2),…” ),…”

Mycoplasma fermentans inhibits tumor Mycoplasma fermentans inhibits tumor necrosis factor alpha-induced necrosis factor alpha-induced

apoptosis.apoptosis.

Mycoplasma fermentans inhibits tumor necrosis Mycoplasma fermentans inhibits tumor necrosis factor alpha-induced apoptosis in the human factor alpha-induced apoptosis in the human myelomonocytic U937 cell line.myelomonocytic U937 cell line. [PMID # [PMID # 15286682, Nov 2004]15286682, Nov 2004]

“To investigate the mechanism of M. fermentans antiapoptotic effect, the reduction of mitochondrial transmembrane potential (DeltaPsim) and the protease activity of caspase-8 were measured. In the infected cells, the reduction of DeltaPsim was inhibited (approximately 75%), and an approximately 60% reduction of caspase-8 activity was measured. In conclusion, M. fermentans significantly inhibits TNFalpha-induced apoptosis in U937 cells, and its effect is upstream of the mitochondria and upstream of caspase-8.”

Induction of in vitro reprogramming by Toll-like Induction of in vitro reprogramming by Toll-like receptor (TLR)2 and TLR4 agonists in murine receptor (TLR)2 and TLR4 agonists in murine

macrophages: effects of TLR "homotolerance" macrophages: effects of TLR "homotolerance" versus "heterotolerance" on NF-kappa B signaling versus "heterotolerance" on NF-kappa B signaling

pathway components, pathway components,

2003 PMID # 124964382003 PMID # 12496438 “TNF-alpha secretion was also suppressed in P. gingivalis LPS-pretreated, Ec LPS-challenged cells, but not vice versa, while Pam3Cys and Ec LPS did not induce a state of cross-tolerance at the level of TNF-alpha. Experiments designed to elucidate novel mechanisms of NF-kappaB inhibition in tolerized cells revealed the potential contribution of IkappaBepsilon and IkappaBxi inhibitory proteins and the necessity of TLR4 engagement for induction of tolerance to Toll receptor-IL-1R domain-containing adapter protein/MyD88-adapter-like-dependent gene expression. Collectively, these data demonstrate that induction of homotolerance affects a broader spectrum of signaling components than in heterotolerance, with selective modulation of specific elements within the NF-kappaB signaling pathway.

Slide 101 -- So, if spirochetes and the mycos (-Slide 101 -- So, if spirochetes and the mycos (-plasma and -bacteria) have the same antigens and plasma and -bacteria) have the same antigens and

we see the same outcomes (low/no antibodies, we see the same outcomes (low/no antibodies, tolerancetolerance to Pam3Cys, to Pam3Cys, anti-apoptosisanti-apoptosis and handling and handling

by TLR2)…by TLR2)…

It became clear that probably these same type of It became clear that probably these same type of lipoproteins were behaving in the same way (which lipoproteins were behaving in the same way (which everyone seems to think are like Pam3Cys or OspA) everyone seems to think are like Pam3Cys or OspA) and if, as was explained to the FDA Vaccine and if, as was explained to the FDA Vaccine Committee in January 2001, the outcomes of LYMErix Committee in January 2001, the outcomes of LYMErix were exactly like “Lyme Disease” as described by were exactly like “Lyme Disease” as described by the patients who had had both, “Lyme Disease” (not the patients who had had both, “Lyme Disease” (not the Steere-knee-only kind of Lyme) and LYMErix, the Steere-knee-only kind of Lyme) and LYMErix, then logically, mechanistically, empirically, and from then logically, mechanistically, empirically, and from the lab outcomes, it appears that Chronic Lyme and the lab outcomes, it appears that Chronic Lyme and LYMErix vaccination produced the same outcomes, LYMErix vaccination produced the same outcomes, like Epstein-Barr-related Multiple Sclerosis, ALS, like Epstein-Barr-related Multiple Sclerosis, ALS, possibly Chronic Fatigue, and cancer.possibly Chronic Fatigue, and cancer.

What was told to the FDA LYMErix Vaccine What was told to the FDA LYMErix Vaccine Committee in January 2001Committee in January 2001

““By what mechanism vaccination of the asymptomatic Bb infected By what mechanism vaccination of the asymptomatic Bb infected patients is causing the Lyme like illness, we do not know exactly. patients is causing the Lyme like illness, we do not know exactly.     Previous infection could be "priming" the immune system, as Previous infection could be "priming" the immune system, as Denise Huber of Tufts has suggested, in "Identification of LFA-1 Denise Huber of Tufts has suggested, in "Identification of LFA-1 as a Candidate Autoantigen in Treatment-Resistent Lyme as a Candidate Autoantigen in Treatment-Resistent Lyme Arthritis"  July 31, 1998, Science, Vol 281, p 703. Arthritis"  July 31, 1998, Science, Vol 281, p 703. ““or the vaccine is activating a dormant infection by the or the vaccine is activating a dormant infection by the immune dysregulation it causesimmune dysregulation it causes, as demonstrated by the , as demonstrated by the effect of Bb infection and Osp A alone, on NK cells population, T effect of Bb infection and Osp A alone, on NK cells population, T cells, cells, neutrophils, and the effects on the various inflammatory neutrophils, and the effects on the various inflammatory regulating biomoleclues, such as IL-10. …regulating biomoleclues, such as IL-10. …    ““We simply don't know all the variables, at present, that We simply don't know all the variables, at present, that effect systemic illness from immune dysregulation caused effect systemic illness from immune dysregulation caused by Bb infection, and especially the effect of a such a a by Bb infection, and especially the effect of a such a a large dose of a known immune irritant, Osp A upon this large dose of a known immune irritant, Osp A upon this system, the asymptomatic Lyme patient. system, the asymptomatic Lyme patient.

““It is because I have gotten so many calls from It is because I have gotten so many calls from patients looking for help because of their illness, patients looking for help because of their illness,

that I am here today.”that I am here today.”

““As a support group leader in Southeastern CT, I have met As a support group leader in Southeastern CT, I have met ~10 people, who found my name on the internet, who had ~10 people, who found my name on the internet, who had adverse events and were ill, looking for help.  After learning adverse events and were ill, looking for help.  After learning more about these patients, I found that all but one of these more about these patients, I found that all but one of these cases had previous Lyme,  and that one got the Erythema cases had previous Lyme,  and that one got the Erythema Migrans rash during the series of vaccination.  NOT ONE Migrans rash during the series of vaccination.  NOT ONE SINGLE PERSON DID NOT HAVE OTHER BANDS ON FOLLOW SINGLE PERSON DID NOT HAVE OTHER BANDS ON FOLLOW UP WESTERN BLOT.     UP WESTERN BLOT.       

““It is because I have gotten so many calls from patients It is because I have gotten so many calls from patients looking for help because of their illness, that I am here looking for help because of their illness, that I am here

today.today.     http://www.fda.gov/ohrms/dockets/ac/01/slides/3680s2_11.http://www.fda.gov/ohrms/dockets/ac/01/slides/3680s2_11.

pdfpdf

We told the FDA that We told the FDA that it looked like Chronic it looked like Chronic Lyme,Lyme, not Steere’s Imaginary “Bad Knees” not Steere’s Imaginary “Bad Knees”

Disease.Disease.

And that And that no one agreed with Allen Steere’s no one agreed with Allen Steere’s new definition of “Lyme Disease” at the new definition of “Lyme Disease” at the Dearborn conference among the invited Dearborn conference among the invited labs.labs.

Mycoplasma fermentans infection promotes Mycoplasma fermentans infection promotes immortalization of human peripheral blood immortalization of human peripheral blood mononuclear cells in culture, mononuclear cells in culture, 2004, PMID 2004, PMID

#15331449#15331449 Blood, 15 December 2004, Vol. 104, No. 13, pp. 4252-4259.Blood, 15 December 2004, Vol. 104, No. 13, pp. 4252-4259.

http://http://bloodjournalbloodjournal..hematologylibraryhematologylibrary.org/.org/cgicgi/content/full/104/13/4252/content/full/104/13/4252

““Our present study revealed that infection of human PBMCs in culture Our present study revealed that infection of human PBMCs in culture with the incognitus and PG18 strains of M fermentans, but with the incognitus and PG18 strains of M fermentans, but surprisingly not with some other strains tested in parallel, markedly surprisingly not with some other strains tested in parallel, markedly enhanced the rate of EBV-positive B lymphocytes to undergo enhanced the rate of EBV-positive B lymphocytes to undergo immortalization (74% vs 17%). Compared with spontaneously immortalization (74% vs 17%). Compared with spontaneously immortalized PBMCs, the PBMCs immortalized in cultures infected immortalized PBMCs, the PBMCs immortalized in cultures infected with the mycoplasmas often had prominent karyotype changes with with the mycoplasmas often had prominent karyotype changes with chromosomal loss, gain, or translocations. Furthermore, many of chromosomal loss, gain, or translocations. Furthermore, many of these immortalized B lymphocytes were found to be monoclonal in these immortalized B lymphocytes were found to be monoclonal in nature. nature. The in vitro findings would be of relevance to The in vitro findings would be of relevance to lymphoproliferative disorders that occurred in patients with lymphoproliferative disorders that occurred in patients with immune suppression. The mycoplasma-mediated immune suppression. The mycoplasma-mediated promotional effect in cell immortalization and its potential promotional effect in cell immortalization and its potential clinical implications warrant further study.clinical implications warrant further study.

(CANCERS)(CANCERS)

The Plum Island Murdered Iraqi scientist (do The Plum Island Murdered Iraqi scientist (do your own research on this… your own research on this… Al-Aubaidi JMAl-Aubaidi JM))

Once Don C. Wiley was bumped off –literally- because he is an Once Don C. Wiley was bumped off –literally- because he is an influenza expert, I began to think it might be true about the influenza expert, I began to think it might be true about the bumped-off former Plum Island Iraqi scientist. His name was bumped-off former Plum Island Iraqi scientist. His name was Al-Aubaidi and he was studying bovine infertility from Al-Aubaidi and he was studying bovine infertility from mycoplasma.mycoplasma.

Joe TullyJoe Tully

Joseph G. Tully and Robert E. Shope (Senior Joseph G. Tully and Robert E. Shope (Senior and Junior). Feel free to use PubMed and and Junior). Feel free to use PubMed and read all of his/their reports.read all of his/their reports.

His interest was in mycoplasma and how His interest was in mycoplasma and how immune dysregulation from mycoplasma immune dysregulation from mycoplasma could play a role in cancer and the like. could play a role in cancer and the like. Now this research is becoming popular Now this research is becoming popular again, so you will see a relative increase in again, so you will see a relative increase in reports on this topic and how Epstein-Barr reports on this topic and how Epstein-Barr is involved in cancer and Multiple Sclerosis.is involved in cancer and Multiple Sclerosis.

Go “Tully JG” without the quotesGo “Tully JG” without the quotes

Tully warns about the lack of interest Tully warns about the lack of interest in Mycoplasma, PMID # 9126441in Mycoplasma, PMID # 9126441

http://iai.asm.org/cgi/content/full/76/1/71/F1http://iai.asm.org/cgi/content/full/76/1/71/F1Mycoplasma adhering to and distorting RBCs. We’re going to guess Mycoplasma adhering to and distorting RBCs. We’re going to guess this plays a role in Chronic Fatigue since CFIDS people have normal this plays a role in Chronic Fatigue since CFIDS people have normal

hemoglobin, but it shore feels like you have must anemia. Dedicated hemoglobin, but it shore feels like you have must anemia. Dedicated VO2-Max exercise efforts to beat the fatigue doesn’t really work… So VO2-Max exercise efforts to beat the fatigue doesn’t really work… So it’s gotta be caused by something like this (RNC membrane osmotic it’s gotta be caused by something like this (RNC membrane osmotic

potential disrupted and therefore O2 potential disrupted and therefore O2 transfer is inhibitedtransfer is inhibited), ), especially since Chronic FatigueLyme and LYMErixFatigue victims now especially since Chronic FatigueLyme and LYMErixFatigue victims now

are are tolerizedtolerized to OspA/Pam3Cys and can’t beat off these fungal to OspA/Pam3Cys and can’t beat off these fungal infections in the blood and elsewhere.infections in the blood and elsewhere.

Slide 110-- Paul Duray in IDSA’s “Reviews,” 1989; Slide 110-- Paul Duray in IDSA’s “Reviews,” 1989; [PMID #2814170], [PMID #2814170], Special Supplement 6 on Lyme Special Supplement 6 on Lyme and Spirochetal Diseases,and Spirochetal Diseases, an entire data set that an entire data set that

IDSA refused to turn over to the CT Attorney IDSA refused to turn over to the CT Attorney General in answer to his subpoena for all of IDSA’s General in answer to his subpoena for all of IDSA’s

previous publications to see if any were missing previous publications to see if any were missing from the IDSA “Guidelines.”from the IDSA “Guidelines.”

If Pam3Cys suppresses the immune system If Pam3Cys suppresses the immune system response by gumming up the auto-kill caspases and response by gumming up the auto-kill caspases and

produces tolerance to mycoplasmal antigens (no produces tolerance to mycoplasmal antigens (no antibodies), and Paul Duray saw what looked like antibodies), and Paul Duray saw what looked like

Epstein-Barr immortalized (mutated, incompetent) Epstein-Barr immortalized (mutated, incompetent) lymphocytes, then we think Chronic Lyme with the lymphocytes, then we think Chronic Lyme with the

Chronic Barbour Star Wars Blebbing or Chronic Barbour Star Wars Blebbing or autovaccination with Pam3Cys is probably the autovaccination with Pam3Cys is probably the cause of the immortalization. And then cancer cause of the immortalization. And then cancer

(leukemia). And MS. And ALS…(leukemia). And MS. And ALS…

““It’s an hypothesis that needs to be tested,” if we could find It’s an hypothesis that needs to be tested,” if we could find some real scientists to do it. Paul Duray is 70-something and some real scientists to do it. Paul Duray is 70-something and deploys to Iraq regularly. Or maybe they have scientists in deploys to Iraq regularly. Or maybe they have scientists in Germany. Germans tend to be smart because their language Germany. Germans tend to be smart because their language is very demanding in logic production. In the US we have the is very demanding in logic production. In the US we have the likes of master debators (Sigal), restaurant sanitation workers likes of master debators (Sigal), restaurant sanitation workers (Fish) and English Literature majors (Shapiro) and genuine (Fish) and English Literature majors (Shapiro) and genuine psychiatric perverts (there are no other kind) trying to tell us psychiatric perverts (there are no other kind) trying to tell us what’s a disease.what’s a disease.

Okay hooduh ferget?, Oh. JJ Halperin and ALS, and Okay hooduh ferget?, Oh. JJ Halperin and ALS, and Gulf War Illness and Mycoplasma, and Sam Donta Gulf War Illness and Mycoplasma, and Sam Donta

and GWIand GWI

1) Justin Radolf on HLA downregulation [11441098] 1) Justin Radolf on HLA downregulation [11441098] [slide 68][slide 68] 2) Ablation of IRAK-associated kinase [15294992] 2) Ablation of IRAK-associated kinase [15294992] 3) Inhibition of TNF-alpha induced apoptosis [15286682] (see also 3) Inhibition of TNF-alpha induced apoptosis [15286682] (see also

Duray)Duray) 4) Inhibition of NF-kappa [12496438]4) Inhibition of NF-kappa [12496438] Alan Barbour on blebbing as autovaccination with Osps Alan Barbour on blebbing as autovaccination with Osps [slide 73][slide 73] Tuberculosis Lipoprotein Vaccine Failures (3) Tuberculosis Lipoprotein Vaccine Failures (3) [slide 75] [slide 75] Gary Wormser on OspA-induced immunosuppression [10865170] Gary Wormser on OspA-induced immunosuppression [10865170] [slide [slide

78]78] McSweegan Attacks McSweegan Attacks [slide 79][slide 79] Paul Duray and EBV-immortalized cells; MS (1989 and 1992) Paul Duray and EBV-immortalized cells; MS (1989 and 1992) [slide 86][slide 86] Joseph G. Tully and mycoplasma in cancer and effect on erythrocytes Joseph G. Tully and mycoplasma in cancer and effect on erythrocytes

(Chronic Fatigue)(Chronic Fatigue) Mycoplasma attaching to RBCs (Chronic Fatigue)Mycoplasma attaching to RBCs (Chronic Fatigue)Halperin on Lyme and ALSHalperin on Lyme and ALSALS and fungal antigensALS and fungal antigensGulf War Illness and ALS (and Sam T. Donta and Mycoplasma and GWI)Gulf War Illness and ALS (and Sam T. Donta and Mycoplasma and GWI) The Murdered Iraqi/ Plum Island Mycoplasmal BioweaponeerThe Murdered Iraqi/ Plum Island Mycoplasmal Bioweaponeer

JJ Halperin - famous promoter of 2 sets of JJ Halperin - famous promoter of 2 sets of “Guidelines” wherein the deadliest version of the “Guidelines” wherein the deadliest version of the

disease, ALS, is not detected, since Dearborn is only disease, ALS, is not detected, since Dearborn is only that imaginary “BAD KNEES DISEASE” that Allen that imaginary “BAD KNEES DISEASE” that Allen Steere concocted in Europe, alone, to set up his Steere concocted in Europe, alone, to set up his

own little RICO with Dave Persing and Robert own little RICO with Dave Persing and Robert Schoen – talks about how Lyme results in ALS in Schoen – talks about how Lyme results in ALS in

47% of the cases:47% of the cases:

PMID: 2334308 Arch Neurol. 1990 May;47(5):586-94.PMID: 2334308 Arch Neurol. 1990 May;47(5):586-94.

“Cerebrospinal fluid was examined in 24 ALS patients--3 (all with severe bulbar involvement) appeared to have intrathecal synthesis of anti-B burgdorferi antibody. Following therapy with antibiotics, 3 patients with predominantly lower motor neuron abnormalities appeared to improve, 3 with severe bulbar dysfunction deteriorated rapidly, and all others appeared unaffected. There appears to be a statistically significant association between ALS and immunoreactivity to B burgdorferi, at least among men living in hyperendemic areas. “

So, I took my widdle pencil and tried to see ifffin there was 5 out So, I took my widdle pencil and tried to see ifffin there was 5 out of 10 Dearborn bands and which 5 out of 10 bands were there… of 10 Dearborn bands and which 5 out of 10 bands were there…

You can see that these people do not have a case of Lyme You can see that these people do not have a case of Lyme disease, yet 9/19 were diagnosed with ALS and later determined disease, yet 9/19 were diagnosed with ALS and later determined

by this gangster to have been exposed to Bb and he said he by this gangster to have been exposed to Bb and he said he thought the 47% association “was significant.”thought the 47% association “was significant.”

Here are the Blots from the 2003 USDOJ RICO Here are the Blots from the 2003 USDOJ RICO complaint. You can see that, clearly, “a complaint. You can see that, clearly, “a

hypersensitivity response to OspA in a knee” will hypersensitivity response to OspA in a knee” will not produce Lyme-ALS-type antibody bands and not produce Lyme-ALS-type antibody bands and

vice versa.vice versa.

ALS and Mycoplasma…and Gulf War ALS and Mycoplasma…and Gulf War Illness and Sam T. Donta. What’s the Illness and Sam T. Donta. What’s the

evidence?evidence?[SUBROUTINE ALSMYCO - slide 1] PMID 12383408, [SUBROUTINE ALSMYCO - slide 1] PMID 12383408,

Garth NicholsonGarth Nicholson

1: J Clin Neurosci. 2002 Sep;9(5):525-9.

High frequency of systemic mycoplasmal infections in Gulf War veterans and civilians with Amyotrophic Lateral Sclerosis (ALS).

“The presence of systemic mycoplasmal infections in the blood of Gulf War veterans (n=8) and civilians (n=28) with Amyotrophic Lateral Sclerosis (ALS) and age matched controls (n=70) was investigated by detecting mycoplasma gene sequences with forensic Polymerase Chain Reaction (PCR) and back hybridization with a radiolabeled internal oligonucleotide probe. Almost all ALS patients (30/36 or approximately 83%) showed evidence of Mycoplasma species in blood samples, whereas <9% of controls had blood mycoplasmal infections (P<0.001).

[SUBROUTINE ALSMYCO - slide 2][SUBROUTINE ALSMYCO - slide 2]

Nicholson, Continued: “Using PCR ALS patients with a positive test for any mycoplasmal infection were investigated for the presence of M. fermentans, M. pneumoniae, M. hominis and M. penetrans in their blood. All Gulf War veterans with ALS were positive for M. fermentans, except one that was positive for M. genitalium. In contrast, the 22/28 civilians with detectable mycoplasmal infections had M. fermentans (13/22, 59%) as well as other Mycoplasama species in their blood, and two of the civilian ALS patients had multiple mycoplasma species (M. fermentans plus M. hominis). Of the few control patients that were positive, only two patients (2/70, 2.8%) were positive for M. fermentans (P<0.001). The results support the suggestion that infectious agents may play a role in the pathogenesis and/or progression of ALS, or alternatively ALS patients are extremely susceptible to systemic mycoplasmal infections.

NIH Determines Conclusively that those deployed to NIH Determines Conclusively that those deployed to the land where we sold Saddam Hussein “fungi” the land where we sold Saddam Hussein “fungi”

and other microorganisms, like Plum Island and other microorganisms, like Plum Island mycoplasma we figger…mycoplasma we figger…

[SUBROUTINE ALSMYCO - slide 3][SUBROUTINE ALSMYCO - slide 3] National Institute of Neurological Disorders and Stroke, National Institute of Neurological Disorders and Stroke,

BethesdaBethesda RESULTS: Among approximately 2.5 million eligible military RESULTS: Among approximately 2.5 million eligible military

personnel, 107 confirmed cases of ALS were identified for an personnel, 107 confirmed cases of ALS were identified for an overall occurrence of 0.43 per 100,000 persons per year. A overall occurrence of 0.43 per 100,000 persons per year. A significant elevated risk of ALS occurred among all deployed significant elevated risk of ALS occurred among all deployed personnel (RR = 1.92; 95% CL = 1.29, 2.84), deployed active personnel (RR = 1.92; 95% CL = 1.29, 2.84), deployed active duty military (RR = 2.15, 95% CL = 1.38, 3.36), deployed Air duty military (RR = 2.15, 95% CL = 1.38, 3.36), deployed Air Force (RR = 2.68, 95% CL = 1.24, 5.78), and deployed Army Force (RR = 2.68, 95% CL = 1.24, 5.78), and deployed Army (RR = 2.04; 95% CL = 1.10, 3.77) personnel. Elevated, but (RR = 2.04; 95% CL = 1.10, 3.77) personnel. Elevated, but nonsignificant, risks were observed for deployed Reserves and nonsignificant, risks were observed for deployed Reserves and National Guard (RR = 2.50; 95% CL = 0.88, 7.07), deployed National Guard (RR = 2.50; 95% CL = 0.88, 7.07), deployed Navy (RR = 1.48, 95% CL = 0.62, 3.57), and deployed Marine Navy (RR = 1.48, 95% CL = 0.62, 3.57), and deployed Marine Corps (RR = 1.13; 95% CL = 0.27, 4.79) personnel. Overall, the Corps (RR = 1.13; 95% CL = 0.27, 4.79) personnel. Overall, the attributable risk associated with deployment was 18% (95% CL attributable risk associated with deployment was 18% (95% CL = 4.9%, 29.4%). CONCLUSIONS: Military personnel who were = 4.9%, 29.4%). CONCLUSIONS: Military personnel who were deployed to the Gulf Region during the Gulf War period deployed to the Gulf Region during the Gulf War period experienced a greater post-war risk of ALS than those who experienced a greater post-war risk of ALS than those who were not deployed to the Gulf. PMID: 14504315 [PubMed - were not deployed to the Gulf. PMID: 14504315 [PubMed - indexed for MEDLINE] indexed for MEDLINE]

Plum Island MycoplasmaPlum Island Mycoplasma[SUBROUTINE ALSMYCO - slide 4] PMID# 6190898 [SUBROUTINE ALSMYCO - slide 4] PMID# 6190898

: J Hyg (Lond). 1983 Jun;90(3):441-9. : J Hyg (Lond). 1983 Jun;90(3):441-9. Immunogenic variation among the so-called LC strains of Immunogenic variation among the so-called LC strains of

Mycoplasma mycoides subspecies mycoides.Mycoplasma mycoides subspecies mycoides. Smith GR, Oliphant Smith GR, Oliphant JC. JC.

““Much evidence of immunogenic heterogeneity among the LC Much evidence of immunogenic heterogeneity among the LC strains of Mycoplasma mycoides ssp. mycoides emerged from strains of Mycoplasma mycoides ssp. mycoides emerged from cross-immunization and -hyper-immunization experiments in cross-immunization and -hyper-immunization experiments in mice in which three LC strains (mice in which three LC strains (Vom/Plum Island, 74/2488, Vom/Plum Island, 74/2488, and Mankefår 2833and Mankefår 2833) were used for challenge purposes. All ) were used for challenge purposes. All heterologous LC-strain vaccines cross-immunized against the heterologous LC-strain vaccines cross-immunized against the three challenge strains, but protection was usually only 'partial', three challenge strains, but protection was usually only 'partial', i.e. significantly less than that given by homologous vaccine. i.e. significantly less than that given by homologous vaccine. Cross-hyperimmunization with all heterologous LC but not SC Cross-hyperimmunization with all heterologous LC but not SC strains produced protection against challenge with Vom/Plum strains produced protection against challenge with Vom/Plum Island that was virtually 'complete', i.e. similar to that produced Island that was virtually 'complete', i.e. similar to that produced by homologous vaccine.by homologous vaccine. … blah blah blah … blah blah blah

Selling fungi to Saddam HusseinSelling fungi to Saddam Hussein [SUBROUTINE ALSMYCO - slide 5][SUBROUTINE ALSMYCO - slide 5]

Congressional Record: September 20, 2002 (Senate) Congressional Record: September 20, 2002 (Senate) Page S8987-S8998 Page S8987-S8998

““Over the protest of some Pentagon skeptics, the Over the protest of some Pentagon skeptics, the Reagan administration began allowing the Iraqis to Reagan administration began allowing the Iraqis to buy a wide variety of "dual use" equipment and buy a wide variety of "dual use" equipment and materials from American suppliers. According to materials from American suppliers. According to confidential Commerce Department export-control confidential Commerce Department export-control documents obtained by NEWSWEEK, the shopping list documents obtained by NEWSWEEK, the shopping list included a computerized database for Saddam's included a computerized database for Saddam's Interior Ministry (presumably to help keep track of Interior Ministry (presumably to help keep track of political opponents); helicopters to transport Iraqi political opponents); helicopters to transport Iraqi officials; television cameras for "video surveillance officials; television cameras for "video surveillance applications"; chemical-analysis equipment for the applications"; chemical-analysis equipment for the Iraq Atomic Energy Commission (IAEC), and, Iraq Atomic Energy Commission (IAEC), and, most most unsettling, numerous shipments of unsettling, numerous shipments of "bacteria/fungi/protozoa" to the IAEC"bacteria/fungi/protozoa" to the IAEC. According . According to former officials, the bacterial cultures could be to former officials, the bacterial cultures could be used to make biological weapons, including anthrax. used to make biological weapons, including anthrax. ……

And of course at the end of Gulf War I we all saw on And of course at the end of Gulf War I we all saw on CNN the US soldiers in Iraq blowing up buried CNN the US soldiers in Iraq blowing up buried

arsenal and the earth moved and there were dust arsenal and the earth moved and there were dust clouds… As if we didn’t see it with our own eyeballs clouds… As if we didn’t see it with our own eyeballs and couldn’t tell that that wasn’t right at the time…and couldn’t tell that that wasn’t right at the time…

We saw it on CNN. Just like we heard Henry We saw it on CNN. Just like we heard Henry Kissinger say on 9/11 on CNN in a phone call from Kissinger say on 9/11 on CNN in a phone call from Europe about 11:30 in the morning (Eastern USA Europe about 11:30 in the morning (Eastern USA Time) that Time) that even the “even the “countries who harbored countries who harbored terrorists”terrorists” would be treated like terrorists and that would be treated like terrorists and that the world the world “was either with us or against us.” “was either with us or against us.”

It happened. The CNN tapes exist.It happened. The CNN tapes exist.

Let’s see. What else do we know Let’s see. What else do we know about OspA and Plum about OspA and Plum StupidStupid

Island?Island?

Epstein-Barr and Multiple Sclerosis… Epstein-Barr and Multiple Sclerosis… HOT HOT HOT HOT HOT!HOT!

Sam T. Donta and Gulf War Sam T. Donta and Gulf War Mycoplasmal OspA/Pam3Cys Illness, Mycoplasmal OspA/Pam3Cys Illness,

PMID # 12057884 PMID # 12057884

““Many veterans who were deployed to the Many veterans who were deployed to the Persian Gulf during the 1990-1991 Gulf War Persian Gulf during the 1990-1991 Gulf War developed multiple unexplained symptoms developed multiple unexplained symptoms such as such as pain, fatigue, and pain, fatigue, and neurocognitive problems.neurocognitive problems. This This constellation of symptoms has been termed constellation of symptoms has been termed Gulf War Veterans' Illnesses (GWVI). Gulf War Veterans' Illnesses (GWVI). Although there is no proven explanation for Although there is no proven explanation for the cause of GWVI, the cause of GWVI, one fairly widespread one fairly widespread explanation is systemic Mycoplasma explanation is systemic Mycoplasma fermentans infection.fermentans infection.

Is there a single definite clue that the ALDF and Yale Is there a single definite clue that the ALDF and Yale knew there was a problem with Chronic Lyme-like knew there was a problem with Chronic Lyme-like adverse events from OspA/Pam3Cys vaccination?adverse events from OspA/Pam3Cys vaccination?

Actually there are at least 3 major clues:Actually there are at least 3 major clues:

1)1) Robert Schoen’s chapter in “Lyme Disease” where he Robert Schoen’s chapter in “Lyme Disease” where he reveals that victims of LYMErix might have the exact same reveals that victims of LYMErix might have the exact same general symptom set as Chronic Lyme victims claim and general symptom set as Chronic Lyme victims claim and that per Schoen these LYMErix victims are to either be that per Schoen these LYMErix victims are to either be blown off or the blood sent to the RICO labs (Imugen and blown off or the blood sent to the RICO labs (Imugen and L2 Diagnostics)L2 Diagnostics)

2)2) As told to the FDA vaccine Committee in Jan 2001, Dave As told to the FDA vaccine Committee in Jan 2001, Dave Persing reported in his RICO patent that vaccine failure Persing reported in his RICO patent that vaccine failure would be indistinguishable from chronic Lymewould be indistinguishable from chronic Lyme

3)3) Gary Wormser in 2001 reported immunosuppression from Gary Wormser in 2001 reported immunosuppression from OspA vaccinationOspA vaccination

GuiltyGuilty

4) The harassment and stalking4) The harassment and stalking of Lyme victims and of Lyme victims and especially Lyme scientists, such as Karen Forschner, Janice especially Lyme scientists, such as Karen Forschner, Janice Beers, Lisa Masterson, Lida Mattman, Bowen Labs, Igenex, Beers, Lisa Masterson, Lida Mattman, Bowen Labs, Igenex, Kathleen Dickson, and several others.Kathleen Dickson, and several others.

5) The false claims by the perpetrators of the crimes that5) The false claims by the perpetrators of the crimes that THEY THEY were being stalked and harassed, which is hilarious were being stalked and harassed, which is hilarious considering we considering we explained the entire crime to the FDA explained the entire crime to the FDA Vaccine Committee in Jan 2001Vaccine Committee in Jan 2001 and there were so and there were so many public hearings including in front of Senator Dodd in many public hearings including in front of Senator Dodd in 1993 where Joe Burrascano described the harassment by 1993 where Joe Burrascano described the harassment by the perps.the perps.

When ever before have the victims of a disease been so When ever before have the victims of a disease been so obviously persecuted along with their treating obviously persecuted along with their treating specialists???specialists???

Slide 127 – 1998, Yale’s Robert Schoen to MDs: Slide 127 – 1998, Yale’s Robert Schoen to MDs: “Blow off LYMErix-injured people with Chronic “Blow off LYMErix-injured people with Chronic Lyme-like symptoms, but otherwise send the Lyme-like symptoms, but otherwise send the

blood to us, one of the RICO Method labs”blood to us, one of the RICO Method labs”

Says Schoeny-RICO-Baloney:Says Schoeny-RICO-Baloney:

““In the absence of specific clinical featuresIn the absence of specific clinical features [according [according to this RICO crew, Lyme Disease is only a bad knee to this RICO crew, Lyme Disease is only a bad knee with no fatigue and dementia signs] with no fatigue and dementia signs] tell the LYMErix tell the LYMErix vaccine damaged person they’re nuts. vaccine damaged person they’re nuts.

““Or else send the blood to the RICO Method labs, Or else send the blood to the RICO Method labs, where we have the No-OspA-B in the Western Blot where we have the No-OspA-B in the Western Blot mix test, something we knew about in 1995 and mix test, something we knew about in 1995 and possibly even before Steere went to Europe, but possibly even before Steere went to Europe, but never told the FDA when falsely presenting LYMErix never told the FDA when falsely presenting LYMErix data.data.

““Thanks and I hope you drop dead and get out of the Thanks and I hope you drop dead and get out of the way (and yer dirty little cockroach kids too!) , way (and yer dirty little cockroach kids too!) , RTSRTS

Do you recall from slide 54 Do you recall from slide 54 Who was Who was HelpinHelpin Persing in 1995 when he was Persing in 1995 when he was

discussin and patentin the RICO method???discussin and patentin the RICO method???

The Persing-Schoen-Steere RICO Method The Persing-Schoen-Steere RICO Method PatentPatent

US Patent No. 6,045,804US Patent No. 6,045,804

“ “Ironically, however, the availability of such Ironically, however, the availability of such vaccines may increase the level of diagnostic vaccines may increase the level of diagnostic uncertainty in the evaluation of patients with uncertainty in the evaluation of patients with presentation of a nonspecific ***flu-like illness*** presentation of a nonspecific ***flu-like illness*** after tick bite or so-called "summer flu," the after tick bite or so-called "summer flu," the majority of which may be due to unrelated causes, majority of which may be due to unrelated causes, to diseases transmitted by ticks such as B. microti, to diseases transmitted by ticks such as B. microti, or to granulocytic Ehrlichia spp. Additional or to granulocytic Ehrlichia spp. Additional uncertainty may arise if the vaccines are not uncertainty may arise if the vaccines are not completely protective; completely protective; vaccinated patients with vaccinated patients with multisystem complaints characteristic of multisystem complaints characteristic of later presentations of Lyme disease may be later presentations of Lyme disease may be difficult to distinguish from patients with difficult to distinguish from patients with vaccine failurevaccine failure. .

Let’s take a closer look at that Persing-Let’s take a closer look at that Persing-Yale RICO method patent claim…Yale RICO method patent claim…

““vaccinated patientsvaccinated patients with with multisystem complaintsmultisystem complaints characteristic ofcharacteristic of later presentations of Lyme disease later presentations of Lyme disease may be difficult to distinguish from patients with may be difficult to distinguish from patients with vaccine failure.”vaccine failure.”

Translation: There are 2 sets of patients:Translation: There are 2 sets of patients:

One set with vaccine damage that looked like chronic late Lyme, andOne set with vaccine damage that looked like chronic late Lyme, andanother set with plain old regular late chronic Lyme.another set with plain old regular late chronic Lyme.

These are not “vague complaints” but the These are not “vague complaints” but the typicaltypical 1) dementia, 1) dementia, confusion, 2) chronic fatigue with sleep disorder, 3) severe confusion, 2) chronic fatigue with sleep disorder, 3) severe headache, light sensitivity, nerve pain or radiculopathy headache, light sensitivity, nerve pain or radiculopathy characteristic of encephalitis with MS/Guillain-Barre, and 4) diffuse characteristic of encephalitis with MS/Guillain-Barre, and 4) diffuse and varying musculoskeletal pain, especially deep muscle pain and varying musculoskeletal pain, especially deep muscle pain and the deep bone pain characteristic of the flu, all of which are and the deep bone pain characteristic of the flu, all of which are verifiable using the correct scientifically valid methods to detect verifiable using the correct scientifically valid methods to detect the ALDF/Yale Biomarkers.the ALDF/Yale Biomarkers.

Reiterating the Four Signs of Reiterating the Four Signs of Typical Chronic Lyme and/or Typical Chronic Lyme and/or

LYMErix DiseaseLYMErix DiseaseThese are not “vague complaints” but the These are not “vague complaints” but the typicaltypical

1) dementia, confusion, 2) chronic fatigue with 1) dementia, confusion, 2) chronic fatigue with sleep disorder, 3) severe headache, light sleep disorder, 3) severe headache, light sensitivity, nerve pain or radiculopathy sensitivity, nerve pain or radiculopathy characteristic of encephalitis with MS/Guillain-characteristic of encephalitis with MS/Guillain-Barre, and 4) diffuse and varying musculoskeletal Barre, and 4) diffuse and varying musculoskeletal pain, especially deep muscle pain and the deep pain, especially deep muscle pain and the deep bone pain characteristic of the flu, all of which are bone pain characteristic of the flu, all of which are verifiable using the correct scientifically valid verifiable using the correct scientifically valid methods to detect the ALDF/Yale scientifically methods to detect the ALDF/Yale scientifically valid Biomarkers of Disease.valid Biomarkers of Disease.

Says Allen Steere in 1989 (before Kaiser-Says Allen Steere in 1989 (before Kaiser-Permanente established the ALDF.com at New York Permanente established the ALDF.com at New York

Medical College), 22% of Medical College), 22% of Chronic Neurologic Chronic Neurologic LymeLyme victims improve with IV treatment, but then victims improve with IV treatment, but then

relapse. PMID # 2172819relapse. PMID # 2172819

““At the time of examination, chronic neurologic At the time of examination, chronic neurologic abnormalities had been present from 3 months to abnormalities had been present from 3 months to 14 years, usually with little progression. Six months 14 years, usually with little progression. Six months after a two-week course of intravenous ceftriaxone after a two-week course of intravenous ceftriaxone (2 g daily), 17 patients (63 percent) had (2 g daily), 17 patients (63 percent) had improvement, improvement, 6 (22 percent) had improvement 6 (22 percent) had improvement but then relapsedbut then relapsed, and 4 (15 percent) had no , and 4 (15 percent) had no change in their condition. CONCLUSIONS. Months to change in their condition. CONCLUSIONS. Months to years after the initial infection with B. burgdorferi, years after the initial infection with B. burgdorferi, patients with Lyme disease may have patients with Lyme disease may have chronic chronic encephalopathy, polyneuropathy, or less encephalopathy, polyneuropathy, or less commonly, leukoencephalitis.commonly, leukoencephalitis. These chronic These chronic neurologic abnormalities usually improve with neurologic abnormalities usually improve with antibiotic therapy. antibiotic therapy.

Pointing out that Steere at one time Pointing out that Steere at one time knew there was something to Lyme knew there was something to Lyme

besides the knee-disease:besides the knee-disease:

““CONCLUSIONS. Months to years after the CONCLUSIONS. Months to years after the initial infection with B. burgdorferi, initial infection with B. burgdorferi, patients with Lyme disease may have patients with Lyme disease may have chronic encephalopathy, chronic encephalopathy, polyneuropathy, or less commonly, polyneuropathy, or less commonly, leukoencephalitis.leukoencephalitis. These chronic These chronic neurologic abnormalities usually improve neurologic abnormalities usually improve with antibiotic therapy.” with antibiotic therapy.”

Duke Biochem- Mechanisms of Duke Biochem- Mechanisms of Immunity graphics (TLR4)Immunity graphics (TLR4)

They haven’t only harmed tick bitees They haven’t only harmed tick bitees worldwide…worldwide…

It should be pretty clear by now that if by the mid-It should be pretty clear by now that if by the mid-1990s, when these ALDF/Yale Lyme crooks knew 1990s, when these ALDF/Yale Lyme crooks knew there was a problem with LYMErix causing a Chronic there was a problem with LYMErix causing a Chronic Lyme-like outcome, and they instead persecuted the Lyme-like outcome, and they instead persecuted the victims of their crimes (while at the same time victims of their crimes (while at the same time playing The Victim Card), and that the outcomes of playing The Victim Card), and that the outcomes of their crimes negatively affected discovery in Multiple their crimes negatively affected discovery in Multiple Sclerosis, ALS, possibly HIV, Gulf War Illness, Cancer Sclerosis, ALS, possibly HIV, Gulf War Illness, Cancer (leukemia), Chronic Fatigue from fungal infections in (leukemia), Chronic Fatigue from fungal infections in the blood, cancer from fungal infections in the the blood, cancer from fungal infections in the blood, immunization of immune-incompetent blood, immunization of immune-incompetent children and finding out the hard way that the kids children and finding out the hard way that the kids were immune-incompetent for over 15 years… that were immune-incompetent for over 15 years… that it wasn’t just people who were bitten by stealth ticks it wasn’t just people who were bitten by stealth ticks who are/were harmed and/or dead from this crime.who are/were harmed and/or dead from this crime.

The Greatest ImitatorThe Greatest Imitator

““vaccinated patients with vaccinated patients with multisystem complaints multisystem complaints characteristic of later characteristic of later presentations of Lyme presentations of Lyme disease may be difficult to disease may be difficult to distinguish from patients distinguish from patients with vaccine failurewith vaccine failure.”.”