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The V. fischeri Autoinducer
N-(-ketocaproyl)-L-homoserine lactone
LuxR
target gene
LuxI LuxR
Quorum Sensing
Acyl-Homoserine Lactone Autoinducers
LuxI-LuxR Quorum Sensing Systems
V. fischeri
P.aeruginosa
A. tumefaciens
E. carotovora
Density dependent luminescence
Virulence factors
Conjugal transfer of Ti plasmid
Antibiotic synthesis
LuxN
LuxQ
H
LuxU
H D
DH
D HTH
LuxO
AI-1
AI-2
Quorum Sensing in V. harveyi
LuxCDABELuxR
LuxP
Quorum Sensing in E. coli and Salmonella
Klebsiella pneumoniae
Lactococcus lactis
Leuconostoc oenos
Listeria monocytogenes
Neisseria gonorrhoeae
Neisseria meningitidis
Pasteurella multocida
Porphyromonas gingivalis
Proteus mirabilis
Salmonella paratyphi
Salmonella typhi
Salmonella typhimurium
Bacillus anthracis
Bacillus halodurans
Bacillus subtilis
Borrelia burgdorferi
Campylobacter jejuni
Clostridium acetobolyticum
Clostridium difficile
Clostridium perfringens
Deinococcus radiodurans
Escherichia coli
Enterococcus faecalis
Haemophilus influenzae
Helicobacter pylori
Shewanella putrefaciens
Staphylococcus aureus
Staphylococcus epidermidis
Streptococcus gordonii
Streptococcus mutans
Streptococcus pneumoniae
Streptococcus pyogenes
Vibrio anguillarum
Vibrio cholerae
Vibrio harveyi
Vibrio vulnificus
Yersinia pestis
Bacteria That Contain luxS
luxS
pfs
metK
MetK
Pfs
Pfs
Page 544
Met
SAM
SAH MTA
SRH MTR
4,5-Dihydroxy-2,3-pentanedione + HC
?
MetK
Pfs Pfs
SAM Utilization
Met
SAM
SAH MTA
SRH MTR
4,5-Dihydroxy-2,3-pentanedione + HC
?
MetK
Pfs Pfs
SAM Utilization
LuxS?
Met
SAM
SAH MTA
SRH MTR
AI-2 + HC ?
MetK
Pfs Pfs
SAM Utilization
LuxS
SRH 4,5-Dihydroxy-2,3-pentanedione
Homocysteine+
The LuxS Reaction
AI-2 Could be a Furanone
MHF DMHF HF DHCP
LuxN
LuxQ
H
LuxU
H D
DH
D HTH
LuxO
AI-1
AI-2
Quorum Sensing in V. harveyi
LuxCDABELuxR
LuxP
Holo-LuxP
Boron is ubiquitous in the biosphere
Boron is required by a number of organisms, but for poorly characterized reasons
Sea water 0.4 mM (primarily boric acid)L.A. tap water 40 μMSoils 2-100 ppmMarine plants 120 ppmLand plants 50 ppmMarine animals 20-50 ppmLand animals 0.5 ppm
Vascular plants cell wall? pollination?DiatomsCyanobacteria heterocyst formationZebrafish embryogenesis
11B NMR
holo-LuxP
filtered
apo-LuxP
Synthesis of AI-2
Boric Acid Induces Lux in V. harveyi
Synthesis of AI-2
Targets of AI-2Vibrio harveyi Bioluminescence, Biofilms
Vibrio cholerae Virulence, Biofilms
Salmonella typhimurium ABC transporter
Salmonella typhi Biofilms
Escherichia coli O157:H7 Type III Secretion (virulence)
Porphyromonas gingivalis Hemin acquisition genes, virulence factors
Shigella flexneri VirB (virulence factor)
Streptococcus pyogenes Secreted protease
Actinobacillus actinomycetemcomitans Periodontal virulence, Mixed-species
biofilms
Photorhabdus luminescens Antibiotic production
Neisseria meningitidis Virulence/bacteremia
Borrelia burgdorferi Virulence
Clostridium perfringens Virulence
Campylobacter jejuni Motility
MicroarraysEscherichia coli K12 ~300 genes Escherichia coli O157:H7 ~400 genesVibrio cholerae El Tor ~70 genes (virulence regulon)
LuxILuxLM
AI-1
Met
SAM
SAH MTA
SRH MTR
AI-2 + HC ?
MetK
Pfs Pfs
LuxS
Biosynthesis of AI-1 and AI-2
LuxN
LuxQ
H
LuxU
H D
DH
D HTH
LuxO
AI-1
AI-2
Quorum Sensing in V. harveyi
LuxCDABELuxR
LuxP