ANALYTICAL BIOCHEMISTRY 246, 270–271 (1997) ERRATA Volume 242, Number 1 (1996), in the article ‘‘The Visualization of Peroxi- somal Proteins Containing a C-Terminal Targeting Sequence on Western Blot by Using the Biotinylated PTS1-Receptor,’’ by Marc Fransen, Chantal Brees, Paul P. Van Veldhoven, and Guy P. Mannaerts, pages 26 – 30: Due to a printer’s error, Fig. 1 on page 28 did not reproduce well. For the reader’s convenience, Fig. 1 and its legend are reprinted here. This erratum is Article No. AB972010. FIG. 1. Visualization of PTS1-proteins on Western blot. One hundred micrograms of proteins, present in rat liver homogenates, subcellular fractions, and purified peroxisomes, was subjected to SDS – PAGE, transferred to nitrocellulose, and analyzed for the presence of PTS1- proteins by using a bacterial lysate containing a vector encoding the biotinylated PTS1-receptor (A, C, and D) or a vector encoding only the biotinylated fusion part (B) as described under Materials and Methods. In C and D, the bacterial lysate was combined with 60 mM SKL- peptide and 60 mM UNSKL-peptide as competitor, respectively. The blue bands are endogenous biotinylated proteins, and the red bands are PTS1-proteins. (H) homogenate, (E) postnuclear supernatant, (N) nuclear fraction, (M) heavy mitochondrial fraction, (L) light mitochondrial fraction, (P) microsomal fraction, (S) cytosolic fraction, (PO) purified peroxisomes, and (PO*) purified peroxisomes of a clofibrate-treated rat. PTS1-containing proteins are indicated by arrows at the right side of D (ACOX-A, the 70-kDa subunit of palmitoyl-CoA oxidase; ACOX- C, the 23-kDa subunit of palmitoyl-CoA oxidase; AGT, L-alanine:glyoxylate aminotransferase 1; MFP, the inducible multifunctional protein; SCPx, the 58-kDa sterol carrier protein; UOX, urate oxidase). Proteins recognized by the anti-SKL antibodies are marked by an asterisk. The migration of molecular weight markers (their mass expressed in kDa) is indicated at the left side of A. 270 Copyright q 1997 by Academic Press All rights of reproduction in any form reserved.

The Visualization of Peroxisomal Proteins Containing a C-Terminal Targeting Sequence on Western Blot by Using the Biotinylated PTS1-Receptor: Volume242,Number 1 (1996), pages 26–30

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Page 1: The Visualization of Peroxisomal Proteins Containing a C-Terminal Targeting Sequence on Western Blot by Using the Biotinylated PTS1-Receptor: Volume242,Number 1 (1996), pages 26–30

ANALYTICAL BIOCHEMISTRY 246, 270–271 (1997)

ERRATA

Volume 242, Number 1 (1996), in the article ‘‘The Visualization of Peroxi-somal Proteins Containing a C-Terminal Targeting Sequence on Western Blotby Using the Biotinylated PTS1-Receptor,’’ by Marc Fransen, Chantal Brees,Paul P. Van Veldhoven, and Guy P. Mannaerts, pages 26–30: Due to a printer’serror, Fig. 1 on page 28 did not reproduce well. For the reader’s convenience,Fig. 1 and its legend are reprinted here. This erratum is Article No. AB972010.

FIG. 1. Visualization of PTS1-proteins on Western blot. One hundred micrograms of proteins, present in rat liver homogenates, subcellularfractions, and purified peroxisomes, was subjected to SDS–PAGE, transferred to nitrocellulose, and analyzed for the presence of PTS1-proteins by using a bacterial lysate containing a vector encoding the biotinylated PTS1-receptor (A, C, and D) or a vector encoding only thebiotinylated fusion part (B) as described under Materials and Methods. In C and D, the bacterial lysate was combined with 60 mM SKL-peptide and 60 mM UNSKL-peptide as competitor, respectively. The blue bands are endogenous biotinylated proteins, and the red bandsare PTS1-proteins. (H) homogenate, (E) postnuclear supernatant, (N) nuclear fraction, (M) heavy mitochondrial fraction, (L) light mitochon-drial fraction, (P) microsomal fraction, (S) cytosolic fraction, (PO) purified peroxisomes, and (PO*) purified peroxisomes of a clofibrate-treatedrat. PTS1-containing proteins are indicated by arrows at the right side of D (ACOX-A, the 70-kDa subunit of palmitoyl-CoA oxidase; ACOX-C, the 23-kDa subunit of palmitoyl-CoA oxidase; AGT, L-alanine:glyoxylate aminotransferase 1; MFP, the inducible multifunctional protein;SCPx, the 58-kDa sterol carrier protein; UOX, urate oxidase). Proteins recognized by the anti-SKL antibodies are marked by an asterisk.The migration of molecular weight markers (their mass expressed in kDa) is indicated at the left side of A.

270Copyright q 1997 by Academic Press

All rights of reproduction in any form reserved.

AID AB ERRA / 6m29$$$481 03-05-97 12:06:49 abca