Thermo Scientific HyperSepProtein Precipitation PlateEffective removal of proteins using theCRASH method

• Hydrophobic/oleophobic frits to enable only precipitation of proteins

• Pore size optimized for consistent flowrate from well to well

• Specially selected polypropylenefor low extractables

Part of Thermo Fisher Scientific

c h r o m a t o g r a p h y

HyperSep™ Protein Precipitation Plate Biological samples commonly contain proteinsthat interfere with downstream analyses. TheThermo Scientific HyperSep Protein PrecipitationPlate uses the CRASH method, in which theprotein is denatured with acetonitrile andthe flocculant filtered out, allowing 96 samples to be processed at one time.

The HyperSep Protein PrecipitationPlate is based on an industry-standard 96-wellplate footprint. Protein ‘crashes’ out of solution and precipitates directly into eachwell when acetonitrile is added. This solvesall common problems associated with theCRASH technique of protein clean-up.Thenovel dual frit, hydrophobically treatedmatrix means that there is no ‘wetting out’and leakage of the sample through the platebefore the application of vacuum. The fritsare Hydrophobic/Olephobic.This retains

sample/acetonitrile in the well to allow precipitation of the proteins only when thevacuum is applied. The pore size of the fritsis optimized to allow ideal flow rate througheach well.

Critical Factors in PerformanceA number of factors are critical to achievinggood separations:

Filter LeakageWhen acetonitrile is added to denature thesample plasma protein, it is important thatthis doesn’t immediately ‘wet out’ the filterand cause the filtration plate to leak.

Flow RateConsistent flow rate of the sample whenthe vacuum applied is a major advantage,as it leads to better reproducibility fromsample to sample.

Product Specifications

Ordering Information

HyperSep Protein Precipitation ProductsDescription Quantity Part Number

Protein Precipitation Plate 1 60304-201

HyperSep SPE ManifoldsDescription Quantity Part Number

HyperSep-96 Vacuum Manifold 1 60103-351Vacuum Pump, European Plug 1 60104-241Vacuum Pump, North American Plug 1 60104-243Universal Vacuum Manifold 1 60104-230

– Accommodates both SPE columns and 96-well plates– System supplied with: manifold, base/gauge, flask & stopper, tubing & spigots

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Product Specifications

SSCCSPROPRPLATE 0510

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Universal Vacuum Manifold

Universal Manifold Base/Gauge

Non Specific Binding of the Compound to Plate/FilterThe very nature of some filters is to bindanalytes; the choice of a non-binding filtermaterial is essential to maximize recoveryof the analytes/compounds.

HyperSep Protein PrecipitationPlate – Method1. Add 400 µL of acetonitrile to the well

2. Wait 1 to 2 minutes to check for filter leakage

3. Rapidly add 100 µL sample to the well

4. Increase vacuum pressure until sampleextraction is visible