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8/9/2019 Thioredoxin in Bacteria
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Multiple catalytically active thioredoxin folds: a winning strategyfor many functions
E milia Pedone Danila Limauro Katia DAmbrosio
Giuseppina De Simone Simonetta Bartolucci
Abstract
ba
Keywords
Introduction
b a
Escherichia
coli
b
a
b
http://scop.mrc-lmb.cam.ac.uk/scop/http://scop.mrc-lmb.cam.ac.uk/scop/8/9/2019 Thioredoxin in Bacteria
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a
cis b
a
K
- -
cis
cis
Fig. 1
a
b f
b c A.
pernix d
e S. solfataricus
f
black b
arrows a
cylinders
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An overview of the PDI family
Saccharomyces cerevisiae
a
b
PDI
b
Fig. 2 white dark grey x
black light grey
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a b
a a0
b b0
b0 a0
x
c
a a0
a a0
a
a
a0
a
a a0
b0
a a0
b0
a a0
b0 a a0
c
a a0
b b0
S C1
S SH
C2
oxidationreduction
S SH
SS
S SH
SS
S
S SH
S S
S SH
S SH
S SH S S
SS
S S
SH SH
S SH
S S
S SHS S
SH
SH SH
SS
S SH
S SH
SSH
S S
S SH
SS
SH SH
S S
A
B
C
SH
Fig. 3white black a b
c C1
C2
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b b0
c
a0
a a0
b b0
a a0
a
a0
K
K
a a0
- -
a a0 K
K
a a0
b0
a
b0
a b
b0x
x b0
xb0
Humicola insolens
a0
a0 b0
a0
E rp57
a
c
Fig. 4 a red b
blue b0 green x a0
yellow
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b0
a0c
a a0
- - a a0
a a0
a a0
bb0
b0
b0
b
b0
b0
b0
b b0
a a0
*
*
a
a a0
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E rp72
a a a0
a
a
a0
a
a
c?
a
a
a
a a0
bb0
bb0
b b0
bb0
a b b0 a0
a
a
a a
PDOs: the discovery of a dual Trx fold in thermophilic
microorganisms
s
Pyrococcus furiosus Aeropyrum pernix
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Aquifex aeolicus Dictyo-
glomus turgidum Roseiflexus
Aquifex Thermotoga
P. furiosus Pf
Pf
A. aeolicus
A. pernix P. furiosus
P. horikoshii Sulfolobus solfataricus
Insight into PDO functions
P. furiosus Pf
P. horikoshii Ph S. solfataricus Ss
A. aeolicus Aa
Ss
S. solfataricus
Ss Pf
Pf
b
a
b
S. solfataricus
Ss Ss
Ss
P. horikoshii
Ph Ph
Fig. 5
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Ss
Ss
Ss
P. furiosus
Pf
P. furiosus
Pf
P. furiosus
pdo, surR
pdo
pdo
P. furiosus
S. solfataricus
Insights into structural features of the PDO family
Pf
Aa
Ap
a
Ss
s
Aa Ap
Pf
K
K
Table 1 K
s
K v v v v0
v0
Aa - -
Aa - -
Pf- - -
Pf - -
Ap - -
Ap - - [
c
Fig. 6 Ap
red cyan
yellow stick
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Ap Aa Pf
K
Ap Aa
Pf
K
Pf
Pf
b0
Similarities and differences between PDI and PDO:
is PDO an ancestor?
Fig. 7
Ph Pyrococcus horykoshii
Pf Pyrococcus
furiosus Aa Aquifex
aeolicus Ap
Aeropyrum pernix
Ss Sulfolobus solfataricus
boxed
bold
underlined
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Heterodimerization of redox-active Trx fold:
hybrid Prxs
b a
S. solfataricus
K
s S. solfataricus
s
Chromatium gracile
Vibrio cholerae
Haemophilus influenzae Neisseria menin-
gitidis
E. coli
C. gracile Cg V. cholerae Vc
N. menigitidis Nm . Cg
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Fig. 8
C. gracile V. cholerae
N. meningitidis
H. influenzae
s
Yersinia aldovae
Serratia
proteamaculans
Erwinia
pyrifoliae
Anabaena
variabilis
Synechoccus sp
R.
sphaeroides
E. coli
Boxed
bold
underlined
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C. gracile
Vc
Cg
Vc
H. influenzae
Hi Hi
Nm
H. influenzae N. meningitidis
Concluding remarks
Fig. 9 Hi green
blue magenta
yellow stick
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References
Escherichia
coli
Escherichia coli
Escherichia coli
Escherichia coli
0
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b
b b
Escherichia coli.
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a
0
0
K
Aeropyrum per-
nix
0
0
0
0
0
0
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Pyrococcus furiosus
Sulfolobus solfataricus
Pyrococcus furiosus
Pyrococcus furiosus
Pyrococcus furiosus
Pyrococcus furiosus
Aquifex aeolicus
Pyrococcus hori-
koshii
Sulfolobus solfataricus
Pyrococcus furiosus
Chlamydomonas reinhardtii
Sulfolobus solfataricus
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Sulfolobus solfataricus
Schizosaccharomyces pombe
Pyrococcus furiosus
Pyrococcus furiosus
j
Sulfolobus solfataricus
Chromatium gracile
Haemophilus influenza
Haemophilus influenzae
Vibrio cholerae
Neisseria menin-
gitidis
Escherichia coli