Upload
others
View
2
Download
0
Embed Size (px)
Citation preview
Page: 1
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Thomas Waeghe, Ph.D. August 16, 2005
Page: 2
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Thomas Waeghe, Ph.D. August 16, 2005
Page: 3
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Thomas Waeghe, Ph.D. August 16, 2005
Page: 4
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Presenter: Thomas Waeghe, Ph.D.
Page: 5
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Choosing Columns and Conditions for LC/MS
Page: 6
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Atmospheric Pressure Ionization (API) LC/MS Techniques
Page: 7
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Relative Applicability of Atmospheric Pressure Ionization Techniques
Page: 8
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Definition and Application of Electrospray Technique
Page: 9
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
ESI Process
Page: 10
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Slide 10
Page: 11
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Key Chromatographic Points in Use of Electrospray
Page: 12
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Definition and Application of APCI Technique
Page: 13
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
APCI and APPI Sources for the LC/MSD
Page: 14
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
AP Chemical Ionization (APCI) Process
Page: 15
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Slide 15
Page: 16
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Key Chromatographic Points in Use of APCI
Page: 17
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Definition and Application of APPI Atmospheric Photoionization
Page: 18
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
APPI Mechanisms
Page: 19
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
APPI Process
Page: 20
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Atmospheric Pressure Ionization Techniques
Page: 21
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Slide 21
Page: 22
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Selecting Columns and Conditions for LC/MS
Page: 23
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Agilent LC/MS Column Configurations
Page: 24
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Choose Longer Columns for Complex Samples
Page: 25
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Choose Longer Columns for Maximum Chromatographic Resolution 2.1 x 150 mm Column
Page: 26
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Choose Shorter Columns for Fast Analysis with MS Identification 2.1 x 50 mm Column
Page: 27
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Choose Short Columns with Sub Two-Micron Particles for Rapid LC/MS APCI Analysis ofClindamycin and...
Page: 28
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Mass Spectra of Clindamycin and Lincomycin High Throughput LC/MS with Rapid Resolution HTColumn
Page: 29
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Solvent Saver 3.0 mm I.D. is Ideal for Converting from LC to LC/MS
Page: 30
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Choose Capillary Columns for LC/MS of Proteins and Peptides Identifiy Peptide PhosphorylationSite...
Page: 31
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Protein Separation on a NanoHPLC Column with Nano-electrospray MS Detection
Page: 32
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Break Number 2
Page: 33
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
LC/MS Mobile Phase Selection - ESI
Page: 34
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Choosing a Volatile Mobile Phase Comparison of Results with Volatile and Non-volatile Mobile PhaseA...
Page: 35
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Comparison Between Volatile Buffer and Non Volatile Phosphate Buffer (pH 2.5) (10 ppminjection:sc...
Page: 36
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Adjust Mobile Phase pH to Ionize Molecules in Solution ESI - LC/MS of Bases and Acids
Page: 37
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Basic Compounds - Benzodiazepines
Page: 38
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Choose Low pH Mobile Phase for Ionized Basic Compounds Positive Ion ESI LC/MS ofBenzodiazepines
Page: 39
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Mobile Phase Buffers for ESI
Page: 40
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
LC/MS Solvent Selection and Guidelines for Successful APCI
Page: 41
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Carbamates by APCI: Extracted Ions
Page: 42
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Electrospray Ionization (ESI) vs. APCI
Page: 43
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
LC/MS Solvent Selection and Guidelines for Successful APPI
Page: 44
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Effect of Chromatographic Solvent on Photoionization of Fat-Soluble Vitamins
Page: 45
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Effect of Dopants on Photoionization of Fat-Soluble Vitamins
Page: 46
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Analysis of Tocopherols (Vitamin E Isomers) by APPI
Page: 47
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Good Chromatography Always Requires Selecting the Best Bonded Phase
Page: 48
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
LC/MS Bonded Phase Selection at Low pH
Page: 49
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
LC/MS Bonded-Phase Selection at Mid pH
Page: 50
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
LC/MS Bonded Phase Selection at High pH – Extend-C18
Page: 51
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Bonded Phase and Mobile Phase Choices for LC/MS Analysis
Page: 52
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Adapting Existing LC Methods to LC/API-MS
Page: 53
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Conclusions
Page: 54
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
HPLC Column Technical Support
Page: 55
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Slide 55
Page: 56
Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Slide 56