Thursday May 1, 2014 Oral Presentations - MCI Groupimgpublic.mci-group.com/nl/ISEV/Abstracts/Thursday_May_1.pdf · Chair: Steve Gould Chair: ... Thursday May 1, 2014 ... of Molecular

*Presenting author

Thursday May 1, 2014


Oral Presentations

07.30-08.30 Registration

07.30-08.30 Arcadis room

Setting up posters (Poster Sessions 4A, 4C ,5A ,5C ,6C ,10B ,11)

10.00-18.00 Sponsor Exhibition Jurriaans Foyer/Mandele room

08.30-10.00 Willem Burger room

Symposium session 4A - EV and tumor development Chair: Aled Clayton

Chair: Peter Kurre

O4A-140 Role of exosome-mediated tumor-stroma interaction in osteosarcoma

Serena Rubina Baglio 1 2,*Tonny Lagerweij 1Amir Avan 1Danijela Koppers-Lalic 1Laura

Roncuzzi 2Nicoletta Zini 2Gloria Bonuccelli 2Michelina Greco 2Benno Naaijkens 1Hans Niessen

1Elisa Giovannetti 1Thomas Wurdinger 1Nicola Baldini 2Dirk Michiel Pegtel 1 1VU University Medical Center, Amsterdam, Netherlands, 2Istituto Ortopedico Rizzoli, Bologna, Italy O4A-141 CHRONIC MYELOID LEUKAEMIA- DERIVED EXOSOMES PROMOTE TUMOUR GROWTH AND SURVIVAL THROUGH AN AUTOCRINE MECHANISM

Stefania Raimondo 1,*Chiara Corrado 1Laura Saieva 1Anna Flugy 1Giacomo De Leo 1Riccardo

Alessandro 1 1Biopatologia e Biotecnologie Mediche e Forensi, University of Palermo, Palermo, Italy O4A-142 PATTERNING THE TUMOR MICROENVIRONMENT VIA EXOSOMAL TRANSMISSION

Jeffrey Franklin 1,*Michelle Demory Beckler 2Jim Higginbotham 2Merrida Childress

3Alexandra Walsh 3Melissa Skala 3Amy-Joan Ham 4Lisa Zimmerman 3Dan Liebler 3Bing Zhang 3Shawn Levy 5Kimberly Watt 3Diana Cha 6Yuanyuan Lu 3Alissa Weaver 3Jim Patton

6Daisuke Hoshino 3Taewan Kim 3Charles Manning 3Seth Karp 3Robert Coffey 3 1Epithelial Biology Center/Department of Cell and Developmental Biology, 2Medicine, 3Vanderbilt University Medical School, 4Belmont University, Nashville, 5HudsonAlpha, Huntsville, 6Vanderbilt University , Nashville, United States O4A-143

*Presenting author


miR-134 transported in extracellular vesicles reduces Triple-Negative Breast Cancer aggression

Keith O'Brien 1,*Claire Corcoran 1Sweta Rani 1John Crown 2Marek Radomski 1Lorraine

O'Driscoll 1 1Pharmacy, Trinity College Dublin, 2Molecular Therapeutics for Cancer Ireland , St. Vincent’s University Hospital, Dublin, Ireland O4A-144 Tumor-derived Exosomes Are Enriched In DNp73 Which Promotes Oncogenic Potential In Acceptor Cells And Correlates With Patient Survival

Marta Rodríguez 1,*Beatriz Soldevilla 1Vanesa García 1Gemma Domínguez 2 1University Hospital Puerta de Hierro-Majadahonda, Majadahonda (Madrid), 2University Autónoma of Madrid, Madrid, Spain O4A-145 Calpain inhibition: a new strategy to circumvent microparticle mediated multidrug resistance in cancer

Ariane Roseblade 1,*Frederick Luk 1Tristan Rawling 1Alison Ung 2Georges E. R. Grau 3Mary

Bebawy 1 1School of Pharmacy, Graduate School of Health, 2School of Chemistry and Forensic Science, Faculty of Science, University of Technology, Sydney, 3Vascular Immunology Unit, Sydney Medical School and Bosch Institute, The University of Sydney, Sydney, Australia

08.30-10.00 Jurriaanse room

Symposium session 4B - EV cargo selection and release mechanisms 1

Chair: Steve Gould

Chair: Willem Stoorvogel

15 minute introduction Clotilde Thery

O4B-146 Dissecting the Wnt-trafficking routes onto extracellular vesicles

Julia Gross 1,*Michael Boutros 1 1Signaling and Functional Genomics, DKFZ, Heidelberg, Germany O4B-147 Live-visualization of exosome release dynamics with a pH-sensitive fluorescent reporter

Frederik Verweij 1,*Juan Garcia-Vallejo 2Serena R. Baglio 1Hans Janssen 3Jacques Neefjes

3Jaap Middeldorp 1Matthijs Verhage 4Ruud Toonen 4Michiel Pegtel 1 1Pathology, VUmc Cancer Center Amsterdam, 2Dept. Molecular Cell Biology & Immunology, VUmc, 3Division of Cell Biology, Dutch Cancer Institute, 4Center for Neurogenomics and Cognitive Research, VUmc, Amsterdam, Netherlands

*Presenting author


O4B-148 Lipid regulators prevent extracellular vesicle budding in C. elegans embryos

Ann Wehman 1,*Jeremy Nance 2 1Rudolf-Virchow-Zentrum, Universität Würzburg, Würzburg, Germany, 2Skirball Developmental Genetics, NYU Langone School of Medicine, New York, United States O4B-149 Senescence-associated exosome suppress tumor growth

Megumi Okada 1Hidetoshi Tahara 2,* 1Hiroshima University Institute of Biomedical & Health Sciences, Hiroshima, Japan, 2Department of Cellular and Molecular Biology, Hiroshima University Institute of Biomedical & Health Sciences, Hiroshima, Japan O4B-150 RAL-1 and the exocyst mediate exosome secretion in C. elegans

Vincent Hyenne 1,*Maxime Diem 1Ahmet Apaydin 1Yannick Schwab 2Michel Labouesse 1 1IGBMC, Illkirch, France, 2EMBL, Heidelberg, Germany

08.30-10.00 Van Weelde/Mees room

Symposium session 4C - EV therapeutics 2

Chair: Raymond Schiffelers

Chair: Yong Song Gho

O4C-151 Transmigration of Cell-Derived Membrane Vesicles in Tumors Mediates Tissue Penetration of Compounds

Ji-Ho Park 1,*Junsung Lee 1Jiyoung Kim 1Moonkyoung Jeong 1 1Department of Bio and Brain Engineering, KAIST, Daejeon, Korea, Republic Of O4C-152 The development of exosomes as a therapy to treat metastatic melanoma

Michael Plebanek 1,*Nicholas Angeloni 2Stephanie Filleur 3Colby S. Thaxton 1Olga Volpert 1 1Urology, Northwestern University, Chicago, IL, 2Urology, Northwestern University, Chicago, 3Urology, Texas Tech University, Lubbock, Texas, United States O4C-153 Extracellular vesicle-mediated reversal of the malignant phenotype in prostate cancer.

Devasis Chatterjee 1,*Michael DelTatto 2David Mills 2Joseph Renzulli 3Peter Quesenberry 2 1Medicine, 2Rhode Island Hospital, 3Urology, Miriam Hospital, Providence, United States O4C-154 Comparison of immunogenicity and antitumor efficacy of two DNA vaccines coding for antigens secreted in different membrane vesicle-associated forms

*Presenting author


clotilde thery 1 2,*christine sedlik 1 2james vigneron 3lea torrieri-dramard 3fabien pitoiset

3jordan denizeau 1caroline chesneau 1philippe de la rochere 1sebastian amigorena 1 2olivier lantz 1 2bertrand bellier 3 1INSERM U932, 2CIC-IGR-Curie-1428, Institut Curie / INSERM, 3UPMC/CNRS UMR7211/INSERM U959, Hôpital Pitié-Salpêtrière, Paris, France O4C-155 Improved therapeutic efficacy of dendritic cell vaccination by loading with leukemia exosomes

Ulrike Erb 1,*Xiaoyu Gu 1Margot Zöller 1 1Tumor Cell Biology, University Hospital of Heidelberg, Heidelberg, Germany

10.00-10.30 Networking coffee Arcadis room

10.00-10.30 Poster Viewing Sessions 4A, 4C, 5A, 5C ,6C ,10B ,11

posters not-attended by authors Arcadis room


Symposium session 5A - EV in infection and immune disorders

Chair: Jeffrey Schorey

Chair: Natasha Barteneva

O5A-157 Legionella pneumophila infection of macrophages alters the amount and miRNA content of released exosomes

Anna Lena Merkel 1,*Kathrin Bolte 2Christina Herkt 1Alexandra Sittka 1Nicoletta Scheller

1Bernd Schmeck 1 1Institute for Lung Research, 2Institute for Cell Biology, Marburg, Germany O5A-158 UBIQUITINATION OF SOLUBLE PROTEINS WITHIN THE PHAGOCYTIC/ENDOCYTIC NETWORK AS A MECHANISM FOR TRAFFICKING TO EXOSOMES

Jeff Schorey 1,*Victoria Smith 1 1Biological Sciences , University of Notre Dame, Notre Dame, United States O5A-159 Extracellular vesicles and small RNA of the cervicovaginal compartment during HIV infection

Kenneth Witwer 1,*Kenzie Birse 2Patrick Tarwater 3Melissa A. McAlexander 4Aleksandra

Olekhnovich 4Diego A. Espinoza 4Joshua Kimani 2 5Charles Wachihi 2 5T B. Ball 2 5 6Adam Burgener 2 6 1Molecular and Comparative Pathobiology, Johns Hopkins University, Baltimore, United States, 2University of Manitoba, Winnipeg, Canada, 3Texas Tech University Health Sciences

*Presenting author


Center, El Paso, 4Johns Hopkins University, Baltimore, United States, 5University of Nairobi, Nairobi, Kenya, 6Public Health Agency of Canada, Winnipeg, Canada O5A-160 Mesenchymal stem cell (MSC)-enriched exosomes mediate beneficial effects in a GvHD patient and in animal models for different inflammation related diseases

Bernd Giebel 1,*Anna-Kristin Ludwig 1Kyra de Miroschedji 1Stefan Radtke 1André Görgens

1Nada Jamjoom 1Vera Rebmann 1Peter A. Horn 1Lambros Kordelas 2Dietrich W. Beelen

2Thorsten R. Döppner 3Josephine Herz 3 4Dirk M. Hermann 3Karla Drommelschmidt 4Ivo Bendix 4Ursula Felderhoff-Müser 4Stefan Landgraeber 5Johannes Rüsing 6Matthias Epple

6Bernhard Singer 7Holger Jastrow 7Sven Brandau 8 1Institute for Transfusion Medicine, 2Department of Bone Marrow Transplantation, 3Department of Neurology, 4Department of Pediatrics, 5Department of Orthopedics, University Hospital Essen, 6Institute of Inorganic Chemistry, University of Duisburg-Essen, 7Institute of Anatomy, 8Department of Otorhinolaryngology, University Hospital Essen, Essen, Germany O5A-161 Unique protein signature of extracellular vesicles in systemic lupus erythematosus

Ole Østergaard 1,*Christoffer T. Nielsen 1Line V. Iversen 1Julia T. Tanassi 1Steen Knudsen

2Søren Jacobsen 3Niels H. H. Heegaard 1 1Department of Clinical Biochemistry, Immunology and Genetics, Statens Serum Institut, Copenhagen, 2Medical Prognosis Institute, Hørsholm, 3Department of Infectious Diseases and Rheumatology, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark O5A-162 Extracellular Vesicles deliver pro-inflammatory viral RNAs (EBV-EBER1) into tubular epithelial cells of lupus nephritis patients

Monique A. Van Eijndhoven 1,*Kirstin M. Heutinck 2Hedy Juwana 1Astrid E. Greijer 1Sandra A.

Verkuijlen 1Ineke R. ten Berge 2Kyra A. Gelderman 1Mary B. von Blomberg 1Katrien Grunberg

1Irene E. Bultink 3Michel Tsang A Sjoe 3Raymond M. Schiffelers 4Alexandre E. Voskuyl 3Jaap M. Middeldorp 1Michiel D. Pegtel 1 1Department of Pathology, Cancer Center Amsterdam, VU University Medical Center, 2department of Internal Medicine, Experimental Immunology and Renal Transplant Unit, Academic Medical Center, 3Department of Rheumatology, VU University Medical Center, Amsterdam, 4Department of Clinical Chemistry and Haematology, UMC Utrecht, Utrecht, Netherlands


Symposium session 5B - EV as cancer biomarkers

Chair: Dolores Di Vizio

Chair: TBA

O5B-163 Blood based cancer diagnostic assay using digital PCR

*Presenting author


Leonora Balaj 1,*Bob Carter 2Sarada Sivaraman 1Johan Skog 3Xandra Breakefield 1Fred

Hochberg 1 1Massachusetts General Hospital, Charlestown, 2USCD, San Diego, 3Exosome Diagnostics, New York, United States O5B-164 Serum exosome miRNA signature as a multi-source biomarker of acute myeloid leukemia

Noah Hornick 1,*Shelton Viola 1Jianya Huan 1Natalya Goloviznina 1Jeff Tyner 2Peter Kurre 1 1Pape Family Pediatric Research Institute, Department of Pediatrics, 2Knight Cancer Institute, Oregon Health & Science University, Portland, United States O5B-165 Highly sensitive pancreatic cancer diagnosis by serum exosome stem cell and miRNA markers Shijing Yue 1Bindhu Madhavan 1Uwe Galli 1Sanyukta Rana 1Wolfgang Gross 2Miryam Müller

1Nathalia A. Giese 3Holger Kalthoff 4Markus W. Büchler 3Margot Zöller 1,* 1Tumor Cell Biology, 2Experimental Surgery at General Surgery, 3General Surgery, University Hospital of Heidelberg, Heidelberg, 4Experimental Cancer Research, University of Kiel, Kiel, Germany O5B-166 Identification of exosome-specific miRNA expression profile in bladder cancer cells and tumor-associated fibroblasts

Sophie Baumgart 1,*Dennis K. Jeppesen 2Bradley Whitehead 3Ken Howard 3Joana

Heinzelmann 1Michael Stoeckle 1Kerstin Junker 1Marie Stampe Ostenfeld 2 1Department of Urology, University Hospital of Saarland, Homburg, Germany, 2Department of Molecular Medicine, 3iNANO, University Hospital, Aarhus, Denmark O5B-167 Characterization of extracellular vesicles in chronic lymphocytic leukemia

Franziska Haderk 1,*Karolin Dorn 1Etienne Moussay 2Jérôme Paggetti 2Guy Berchem 2Peter

Lichter 1Martina Seiffert 1 1Molecular Genetics, German Cancer Research Center, Heidelberg, Germany, 2Experimental Hemato-Oncology, CRP Santé , Luxembourg, Luxembourg O5B-168 Changes in subpopulations of extracellular vesicles by oncogene-targeted treatment of Melanoma cells

Taral Lunavat 1,*Cecilia Lässer 1Jonas Nilsson 2Jan Lötvall 1 1Krefting Research Centre, Department of Internal Medicine, 2Sahlgrenska Cancer Centre, Department of Surgery, University of Gothenburg, Göteborg, Sweden


Symposium session 5C - EV in tissue repair & remodelling

*Presenting author


Chair: Giovianni Camussi

Chair: Hans van Leeuwen

O5C-169 Osteoblast-secreted extracellular vesicles stimulate the expansion of CD34+ human umbilical cord blood cells

Jess Morhayim 1,*Jeroen Demmers 2Andre van Wijnen 3Eric Braakman 4Jan Cornelissen

4Jeroen van de Peppel 1Hans van Leeuwen 1 1Internal Medicine, 2Proteomics Center, Erasmus Medical Center, Rotterdam, Netherlands, 3Orthopedic Surgery & Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States, 4Hematology, Erasmus Medical Center, Rotterdam, Netherlands O5C-170 Age-dependent loss of microvesicular Galectin-3 and its consequences on bone formation in vitro and in vivo

Sylvia Weilner 1,*Dapi M.-L. Chiang 2Patrick Heimel 3Verena Keider 1Elisabeth Schraml

1Florian Weiß 1Regina Grillari-Voglauer 4Heinz Redl 3Huan Y. Chen 2Johannes Grillari 1 1Department für Biotechnologie, University of Natural Resources and Life Sciences, Vienna, Vienna, Austria, 2Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Province of China, 3Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, 4Evercyte GmbH, Vienna, Austria O5C-171 Reversal of hematopoietic radiation damage by mesenchymal stem cell-derived extracellular vesicles

Sicheng Wen 1,*Laura Goldberg 1Mark S. Dooner 1John L. Reagan 1Elaine Papa 1Michael Del

Tatto 1Mandy Pereira 1Arina Sorokina 1Yanhui Deng 1Jason Aliotta 1Peter Quesenberry 1 1Hematology/Oncology, Brown University/Rhode Island Hospital, Providence, United States O5C-172 Impaired expression of miRNAs in mesenchymal stem cell-derived EVs reduced their regenerative potential in a model of acute kidney injury

Federica Collino 1,*Stefania Bruno 2Daniela Dettori 3Ciro Tetta 4Giovanni Camussi 1 1Dept. Medical Science-Translation center of Regenerative Medicine, 2Dept. Molecular Biotechnology and Healthy Sciences, University of Torino, 3Human Genetics Foundation (HuGeF), 4Translation center of Regenerative Medicine, Fresenius Medical Care Deutschland GmbH, Torino, Italy O5C-173 Enhancement of cell-proliferation by artificial exosomes engineered from embryonic stem cells

Dayeong Jeong 1,*Wonju Jo 2Junho Kim 1Jaesung Park 1 2 1School of Interdisciplinary Bioscience and Bioengineering, 2Dept. Mechanical Engineering , POSTECH, Pohang, Korea, Republic Of

*Presenting author


O5C-174

Dual Activation of Akt and β-catenin Signaling by Exosomes from Human Umbilical Cord

Mesenchymal Stem Cells Enhances Wound Healing Zhang Bin 1Gong Aihua 1Zhang Xu 1Wang Mei 1Shi Hui 1Zhu Yanhua 1Wu Lijun 1Qian Hui 1Xu

Wenrong 1,* 1Jiangsu University, Zhenjiang, China

12.00-13.00 Networking lunch Arcadis room

12.30-13.00 Poster Viewing Sessions 4A,4C,5A,5C,6C,10B,11

attended by authors Arcadis room

13.00-14.00 Poster Walk, by chairperson, Session 4C,5A,5C,6C,10B,11

Posters attended by authors Arcadis room


Symposium session 6A - EV analysis by flow cytometry

Chair: Frank Coumans

Chair: Marca Wauben

O6A-233 Enumeration of extracellular vesicles by a new improved flow cytometric method is comparable to fluorescence mode nanoparticle tracking analysis

Leonardo Pasalic 1,*Rebekka Williams 2Heather Campbell 1Agnieszka Siupa 3Michelle J.

Henderson 2Vivien Chen 1 4 1Prince of Wales Clinical School, University of New South Wales, 2Experimental Therapeutics Program, Children’s Cancer Institute Australia for Medical Research, Sydney, Australia, 3NanoSight Ltd, Amesbury, United Kingdom, 4South Eastern Area Laboratory Services, Prince of Wales Hospital, Sydney, Australia O6A-234 Comparative analysis of extracellular vesicles and reference particles using high-resolution flow cytometry, tunable resistive pulse sensing, and nanoparticle tracking analysis

Sybren L. N. Maas 1 2,*Jeroen De Vrij 1 2Els J. Van der Vlist 3Biaina Geragousian 1 2Raymond M.

Schiffelers 4Ger J. A. Arkesteijn 5Marike L. D. Broekman 1 2Marca H. M. Wauben 3Esther N. M. Nolte-’t Hoen 3 1Department of Neurosurgery, 2Brain Center Rudolf Magnus, University Medical Center Utrecht, 3Department of Biochemistry & Cell Biology, Fac Veterinary Medicine, Utrecht University, 4Department of Clinical Chemistry and Haematology, University Medical Center Utrecht, 5Department of Infectious Diseases & Immunology, Fac Veterinary Medicine, Utrecht University, Utrecht, Netherlands

*Presenting author


O6A-235 Purification of extracellular vesicles by high resolution flow cytometric sorting

Ger J. A. Arkesteijn 1,*Tom Groot Kormelink 2Sten F.W.M. Libregts 2Willem Stoorvogel 2Esther

N. M. Nolte-'t Hoen 2Marca H. M. Wauben 2 1I&I, Immunology , 2Biochemistry and Cell Biology, Utrecht University, Veterinary Medicine, Utrecht, Netherlands O6A-236 nanoFACS: Flow Cytometric Analysis and Sorting of Single Extracellular Vesicles

Jennifer Jones 1,*William Telford 2Marty Bigos 3Vasilis Toxavidis 4John Tigges 4E J. Felton

4Kathy McKinnon 1Susan J. Knox 3Jay Berzofsky 1 1National Cancer Institute, Vaccine Branch, 2NCI/EITB, National Institutes of Health, Bethesda, 3Stanford University, Stanford, 4BIDMC, Harvard University, Boston, United States O6A-237 Luminal labeling of extracellular vesicles: advantages and pitfalls

Susan Van Dommelen 1,*Sander Kooijmans 1Pieter Vader 1 2Raymond Schiffelers 1 1Department of Clinical Chemistry and Haematology, UMC Utrecht, Utrecht, Netherlands, 2Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom O6A-238 Differentiation of microvesicles in whole blood with respect to cellular origin

René Weiss 1,*Andreas Spittler 2Viktoria Weber 1Michael B. Fischer 1 1Center for Biomedical Technology, Christian Doppler Laboratory for Innovative Therapy Approaches in Sepsis, Krems, 2Core Facility Flow Cytometry, Surgical Research Laboratory, Vienna, Austria


Symposium session 6B - Clinical implications of EV in (cardio)vascular disorders

Chair: Joan Joseph

Chair: Peter Quesenberry

O6B-241 Comparative analysis of extracellular vesicle-based miRNA isolated from mice with monocrotaline-induced pulmonary hypertension and humans with pulmonary arterial hypertension

Jason Aliotta 1,*Mandy Pereira 1Mark S. Dooner 1Laura Goldberg 1Sicheng Wen 1Peter J.

Quesenberry 1 1Medicine, Rhode Island Hospital/Alpert Medical School of Brown University, Providence, United States

*Presenting author


O6B-242 Plasma exosomes as mediators of remote ischemic preconditioning: protecting the heart at a distance

Jose Vicencio 1,*Derek M. Yellon 1Sean M. Davidson 1 1Hatter Cardiovascular Institute, UCL, London, United Kingdom O6B-243 Comparison of calcifying exosomes, calciprotein particles and blood-derived vesicles reveals distinct morphological appearance and biological properties

Alexander Kapustin 1,*Sundeep Kalra 1Karin Muller 2Jeremy N. Skepper 2Ying Zheng 3Sean M.

Davidson 3Catherine M. Shanahan 1 1BHF Centre of Research Excellence, King's College London, London, 2Department of Physiology, Development and Neuroscience, Cambridge University, Cambridge, 3Hatter Cardiovascular Institute, University College London, London, United Kingdom O6B-244 Extracellular vesicle-derived CD14 is independently associated with the extent of cardiovascular disease burden in patients with manifest vascular disease

Dominique De Kleijn 1,*Joyce Vrijenhoek 2Frank Visseren 3Frans Moll 4Gert-Jan de Borst

4Michiel Bots 5Louise Catanzariti 2Sander van de Weg 2Gerard Pasterkamp 2Hester den Ruijter 6 1CVRI NUHS, & Cardiology UMCU, Surgery NUS, CVRI NUHS, Singapore; UMC Utrecht & ICIN, the Netherlands, Singapore, Singapore, 2Cardiology UMC Utrecht, 3Vascular Medicine, 4Vascular Surgery, 5Julius Center, 6Cardiology & Julius Center, UMC Utrecht, Utrecht, Netherlands O6B-245 Effects of Antiplatelet Therapy on Platelet Extracellular Vesicles in Health and Disease

David Connor 1 2Ken Ly 1Anoosha Aslam 1Joyce Low 3Susan Jarvis 3Joanne Joseph 1 2,* 1Haematology Research Laboratory, St Vincent's Centre for Applied Medical Research, Darlinghurst, 2Faculty of Medicine, University of New South Wales, Sydney, 3Haemostasis Laboratory, Sydpath, St Vincent's Hospital, Darlinghurst, Australia O6B-246 Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Prevent Cisplatin-Induced Nephrotoxicity by Activating Autophagy Wang Bingying 1Yan Yingmin 1Jia Haoyuan 1Zhang Xu 1Wang Mei 1Zhang Bin 1Xu Wenrong

1Qian Hui 1,* 1Jiangsu University, Zhenjiang, China


Symposium session 6C - EV as biomarkers

Chair: Hidetoshi Tahara

Chair: Shona Pedersen

*Presenting author


O6C-249 Advanced Molecular Tools for Proteomic Analyses of Microvesicles

Masood Kamali-Moghaddam 1,*Liza Löf 1Felipe Felipe Oliveira 1Lotta Wik 1Di Wu 1Junhong

Yan 1 1Dept. of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden O6C-250 CHARACTERIZATION OF EXOSOME-LIKE VESICLES IN UTERINE ASPIRATES

Marina Rigau 1,*Irene Campoy 1Lucia Lanau 1Tatiana Altadill 1Tamara Sequeiros 1Melania

Montes 1Mireia Olivan 1Andreas Doll 1Maria E. Suárez 2Montserrat Cubo-Albert 2Silvia Cabrera 2Jose Luis Sánchez-Iglesias 2Berta Díaz-Feijoo 2Assumpció Pérez-Benavente 2Antonio Gil-Moreno 2Marta Llauradó 3Jaume Reventós 1Eva Colás 1 1Research Unit in Biomedicine and Translational Oncology, Vall Hebron Institute of Research, 2Department of Gynecological Oncology, Vall Hebron University Hospital, Barcelona, Spain, 3Faculty of Medicine, University of British Columbia, Vancouver, Canada O6C-251 Proteomic analysis of urinary extracellular vesicles in ADPKD patients

Mahdi Salih 1,*Jeroen A. Demmers 1Karel Bezstarosti 1Ewout J. Hoorn 1Robert Zietse 1 1Internal medicine - Division of Nephrology & Transplantation, Erasmus MC, Rotterdam, Netherlands O6C-252 IMPACT OF AGE ON URINARY EXTRACELLULAR VESICLES FROM HEALTHY HUMANS

Muthuvel Jayachandran 1,*Wing Lam 1Virginia M. Miller 1John C. Lieske 2Vesna D. Garovic

2Andrew D. Rule 2 1Surgery, Physiology and Biomedical Engineering, 2Internal Medicine and Division of Nephrology & Hypertension, Mayo Clinic, Rochester, United States O6C-253 Comparative analysis of plasma and plasma EVs for identification of preterm birth biomarkers

Kok Hian Tan 1,*Soon Sim Tan 2Grace Ng 3Mor Jack Ng 4Sai Kiang Lim 2 1Department of Maternal Fetal Medicine, KK Women's & Children's Hospital, 2Institute of

Medical Biology, A*STAR, 3Division of O&G, KK Women’s and Children’s Hospital, 4SingHealth Duke-NUS OBGYN Academic Clinical Program, Singapore, Singapore O6C-254 PROTEOMICS AND METABOLOMICS ANALYSES TO UNRAVEL NOVEL MARKERS AND PHYSIOLOGICAL ROLE OF HEPATOCYTE-DERIVED EV

Juan M Falcon-Perez 1,*

*Presenting author


1Metabolomics Unit, CIC bioGUNE, CIBERehd, IKERBASQUE Research Foundation, Derio, Spain

16.00-16.30 Networking coffee Arcadis room

16.00-16.30 Poster Viewing Sessions 4A,4C,5A,5C,6C,10B,11

posters not-attended by authors Arcadis room


Plenary Session

Chair: Clotilde Thery

Chair: Jan Lötvall

Exosomes: Looking back three decades and into the future

Phil Stahl

19.00-22.00 ISEV Networking dinner & Social Event

The Networking Dinner will take place aboard the largest paddle

steamer of Europe "De Majesteit".

*Presenting author


Poster Presentations 13.00-14.00 Arcadis room

Poster Session 4A - EV in cancer 1

Chair: Irina Nazarenko

Chair: Aris Panaretakis

P4A-175 Extraction and characterization of vesicles from patient samples in Helsinki urological biobank

Maija Puhka 1,*Taija af Hällström 1Tuomas Mirtti 2Kimmo Pitkänen 1Antti Rannikko 3Olli

Kallioniemi 1 1Helsinki Urological Biobank, Institute for Molecular Medicine Finland FIMM, 2Department of Pathology, 3Department of Urology, Helsinki University Central Hospital, Helsinki, Finland P4A-176 Exosome-Based Serum Analysis Reveals Reliable Cancer Tissue-Related miRNA Signatures

Abdou Elsharawy 1,*Christian Röder 2Stefan Schreiber 1 3Philip Rosenstiel 1Holger Kalthoff 2 1Institute of Clinical Molecular Biology (ICMB), Center of Molecular Bioscience (CMB), Christian-Albrechts-University (CAU), 2Division of Molecular Oncology, Institute for Experimental Cancer Research, University Hospital Schleswig-Holstein, Campus Kiel, 3Clinic for Internal Medicine I, University Hospital of Schleswig-Holstein, Campus Kiel, Kiel, Germany P4A-177 Exosomes; Future Diagnostic Tool in Prostate Cancer

Elham Hosseini-Beheshti 1,*Wendy Choi 2Hans Adomat 2Emma S. Tomlinson Guns 3 1Experimental Medicine, 2The Vancouver Prostate Centre- University of British Columbia, Vancouver, Canada, 3Urologic Sciences, The Vancouver Prostate Centre- University of British Columbia, Vancouver, Canada P4A-178 Prostate cancer cell–derived extracellular vesicle subpopulations contain different gDNA fragments

Elisa Lázaro-Ibáñez 1,*Andrés Sanz-García 1Carmen Escobedo-Lucea 1Pia Siljander 1 2Ángel

Ayuso-Sacido 1 3Marjo Yliperttula 1 1Division of Pharmaceutical Biosciences, Department of Biopharmaceutics, 2Division of Biochemistry and Biotechnology, Department of Biosciences, University of Helsinki, Helsinki, Finland, 3IMMA-CIOCC, Fundación Hospital de Madrid , Madrid, Spain P4A-179 Potential of the mRNA carried by urinary extracellular vesicles for the diagnosis of prostate and bladder cancers

*Presenting author


Felix Royo 1,*Patricia Zuñig 2Amparo Perez 3Ana Loizaga 3Raquel Arceo 3Isabel Lacasa 3Ainara

Rabade 3Kerman Zorroza 4David Mosen-Ansorena 5Esperanza Gonzalez 1Ana M. Aransay

5Miguel Unda-Urzaiz 3Arkaitz Carracedo 2Juan M. Falcon-Perez 1 1Metabolomics unit, 2Proteomics Unit, CIC bioGUNE, Derio, 3Urology Service, Basurto University Hospital, 4Basque Foundation for Health Innovation and Research (BIOEF), DNA Laboratory, Basurto Hospital, Bilbao, 5Genome Analysis Platform, CIC bioGUNE, Derio, Spain P4A-180 Procoagulant activity of microparticle from patient with acute Leukemia has a predictive value for thrombotic events

Damien Gheldof 1,* 1Hematology Laboratory-Narilis Chu Mont-Godinne, Université Catholique De Louvain, Namur, Belgium P4A-181 Exosomal biomarkers for primary and recurrent lung cancer

Radha Munagala 1 1,*Farrukh Aqil 1Ramesh C. Gupta 2 1Dept. of Medicine and James Graham Brown Cancer Center, 2Dept. of Pharm/Tox and James Graham Brown Cancer Center, University of Louisville, Louisville, United States P4A-182 Characterization of circulating microparticle origin in patients with myeloproliferative neoplasms (MPN) by flow cytometry

Carmen Julia Tartari 1,*Marina Marchetti 1Romaric Lacroix 2Laura Russo 1Francesca Piras

1Guido Finazzi 3Alessandro Rambaldi 3Anna Falanga 1 1Division of Immunohematology and Transfusion Medicine, Hospital Papa Giovanni XXIII, Bergamo, Italy, 2Hematology and Vascular Biology Department, Hospital La Conception, Marseille, France, 3Division of Hematology, Hospital Papa Giovanni XXIII, Bergamo, Italy P4A-183 Proteomic analysis of exosomes from highly metastatic and non-metastatic murine breast cancer cell lines

Sophia Ceder 1,*Theocharis Panaretakis 1 1Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden P4A-184 Plasma extracellular vesicles are not suitable to detect tumour specific mutations

Kirsten Krätzel 1,*Florian S. Dreyer 1Elisabeth Kremmer 2Reinhard Zeidler 2Ian Johnston

3Andreas Baur 1 1Department of Dermatology, Universitätsklinikum Erlangen, Erlangen, 2Helmholtz Zentrum, München, 3Miltenyi Biotec GmbH, Bergisch Gladbach, Germany

*Presenting author


P4A-185 Different Exosome Cargo From Plasma/Bronchoalveolar Lavage In Non-Small-Cell Lung Cancer

Vanesa García 1,*Javier Silva 1Marta Rodríguez 1Félix Bonilla 1 1University Hospital Puerta de Hierro-Majadahonda, Majadahonda (Madrid), Spain P4A-1860 Phenotyping of exosomes from various cancer cell lines using extracellular vesicle (EV) Array and nanoparticle tracking analysis (NTA)

Evo K. L. Søndergaard 1,*Lotte H. Pugholm 1Malene Jørgensen 1Rikke Bæk 1Shona Pedersen

2Søren R. Kristensen 2Kim Varming 1 1Clinical Immunology, 2Clinical Biochemistry, Aalborg University Hospital, Aalborg, Denmark

P4A-1861 Expression of surface antigens in subpopulations of exosomes released into the urine from prostate cancer patients Axl Neurauter 1,*Anette Kullmann 1Kjetil W. Pedersen 1Morten Oksvold 2

1Research and Development, Life Technologies, 22Centre for Cancer Biomedicine, University of Oslo, Oslo, Norway


Poster Session 4C - EV therapeutics 2

Chair: Ramesh Gupta

Chair: Soon Sim Tan

P4C-187 BIODISTRIBUTION OF MILK-DERIVED EXOSOMES AND EXOSOMAL-CURCUMIN

Farrukh Aqil 1,*Radha Munagala 1Jeyaprakash Jeyabalan 2Ramesh C. Gupta 3 1Department of Medicine, JG Brown Cancer Center, 2JG Brown Cancer Center, 3Department of Pharmacology and Toxicology, JG Brown Cancer Center, University of Louisville, Louisville, United States P4C-188 Biodistribution of Exosomes: Evaluation of different cell sources, doses and time points

Oscar Wiklander 1,*Joel Nordin 1Aisling O''Loughlin 2Yi Lee 2Lydia Alvarez-Erviti 3Imre Mäger

2 4Pieter Vader 2C.I. Edvard Smith 1Matthew J. Wood 2Samir El Andaloussi 1 2 1Laboratory medicine, Karolinska institutet, Stockholm, Sweden, 2Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, 3Department of Clinical Neuroscience, Institute of Neurology, University College London, London, United Kingdom, 4Institute of Technology, University of Tartu, Tartu, Estonia P4C-189 Extracellular vesicles for delivery of small RNAs

Pieter Vader 1,*Yi Lee 1Imre Mäger 1Samir EL Andaloussi 2Matthew Wood 1

*Presenting author


1University of Oxford, Oxford, United Kingdom, 2Karolinska Institute, Huddinge, Sweden P4C-190 Extracellular vesicle-mediated delivery of components of a new prodrug therapeutic regimen

Daniel O. Frimannsson 1AC Matin 1,*Jing-Hung Wang 1Mark D. Pegram 2Alain Delcayre

3Bradley Efron 4Stefanie Jeffrey 5 1Microbiology and Immunology, 2Department of Medicine , Stanford University, Stanford, 3Exothera LLC, Menlo Park, 4Department of Statistics, 5Department of Surgery, Stanford University, Stanford, United States P4C-191 The evaluation of a hybrid extracellular vesicle-polymer nanocomposite for RNAi therapy

Stephan Stremersch 1,*Kevin Braeckmans 1Stefaan De Smedt 1Koen Raemdonck 1 1Lab of general biochemistry and physical pharmacy, University Ghent, ghent, Belgium P4C-192 Functional Polymer Gel-Exosomes Hybrids for Drug Delivery System and Tissue engineering Shin-ichi Sawada 1 2Junichi Yasuoka 2Yuko Sato 1 2Asako Shimoda 1 2Naohiro Seo 3Nozomi

Harada 3Hiroshi Shiku 3Kazunari Akiyoshi 1 2,* 1Kyoto University, 2JST-ERATO, Kyoto, 3Mie University, Mie, Japan


Poster Session 5A - EV in infection and immune disorders

Chair: TBA

Chair: Francesc Borràs

P5A-193 The Effect of Rosuvastatin on Circulating Microparticles in Treatment-Naïve HIV-Patients

Robyn G. Weijma 1,*Muriel van Schilfgaarde 2Eric A. Vos 1Lea M. Dijksman 3Anja Leyte 2Guido

E. van den Berk 1Kees Brinkman 1Jos H. Frissen 1Ineke E. Schouten 1Wim E. Terpstra 1Willem L. Blok 1 1Internal medicine, 2Haematology and Clinical Chemistry, 3Teaching Hospital, Olvg, Amsterdam, Netherlands P5A-194 Differences in complement factors on BAL fluid exosomes between sarcoidosis patients and healthy controls

Maria-Jose Martinez-Bravo 1,*Khaleda R. Qazi 1Casper Wahlund 1Benita Dahlberg 2Robert

Moulder 3Anders Eklund 2Johan Grunewald 2Susanne Gabrielsson 1 1Department of Medicine Solna Translational Immunology Unit, 2Department of Medicine Solna Division of Respiratory Medicine, Karolinska Institutet, Stockholm, Sweden, 3Turku Centre for Biotechnology, University of Turku, Turku, Finland

*Presenting author


P5A-195 EXOSOMES FROM EPSTEIN-BARR VIRUS INFECTED GASTRIC CELLS CONTAIN EBV MIR-BART15-3P TO TARGET ANTI-APOPTOTIC TAX1BP1 GENE

Hoyun Choi 1Yu-jin Jung 1Suk Kyeong Lee 1,* 1Department of Medical Lifescience, The Catholic University of Korea, Seoul, Korea, Republic Of P5A-196 Elevated levels of TNFa contained extracellular vesicles in HAART treated HIV patients

Jung-Hyun Lee 1,*Jochen Dindorf 1Florian Dreyer 1Thomas Harrer 2Nina Koliha 3Stefan Wild

3Andreas Baur 1 1Department of Dermatology, 2Department of Immunology, University of Erlangen, Erlangen, 3Miltenyi Biotec GmbH, Bergisch Gladbach, Germany P5A-197 HIV-infected and Nef transfected microglia and astrocytes secrete exosomes carrying Nef

Jana Ferdin 1,*Pia Puzar Dominkus 1Ana Plemenitas 1Matija Peterlin 1 2Metka Lenassi 1 1Institute of Biochemistry, University of Ljubljana, Faculty of Medicine, Ljubljana, Slovenia, 2Department of Medicine, University of California, San Francisco, San Francisco, United States P5A-198 Exosomes from Human Immunodeficiency virus type-1 (HIV-1)-infected cells license quiescent CD4+ T lymphocytes to replicate HIV-1 through a Nef- and ADAM17-dependent mechanism

Claudia Arenaccio 1,*Chiara Chiozzini 1Sandra Columba Cabezas 2Elisabetta Affabris 3Andreas

Baur 4Maurizio Federico 1 1National AIDS Center, 2Cell Biology and Neurosciences, Istituto Superiore di Sanità, 3Department of Science, University of Roma TRE, Rome, Italy, 4Department of Dermatology, University Hospital Erlangen , Erlangen, Germany P5A-199 Extracellular vesicles from Haemophilus influenzae-infected macrophages induce pro-inflammatory responses

Charlotte Volgers 1,*Birke Benedikter 1Paul Savelkoul 1Frank Stassen 1 1Medical Microbiology, Maastricht University, Maastricht, Netherlands P5A-200 The role of exosomes in the inflammatory response to high intensity exercise

Ryan Pink 1,*Eman Abu-Seer 1David Carter 1Helen Dawes 1 2 1Department of Biological and Medical Sciences, Oxford Brookes University, 2Department of Clinical Neurology, University of Oxford, Oxford, United Kingdom

*Presenting author


P5A-201 Cigarette smoke extract induces airway epithelial cells to secrete a compositionally and functionally distinct population of extracellular vesicles

Birke Benedikter 1,*Charlotte Volgers 1Paul Savelkoul 1Gernot Rohde 2Frank Stassen 1 1Medical Microbiology, 2Respiratory Medicine, Maastricht University Medical Center, Maastricht, Netherlands P5A-202 Characterization of neutrophilic granulocytes derived apoptotic bodies

Akos Lorincz 1,*Maria Schutte 1Csaba Timar 1Erzsebet Ligeti 1 1Semmelweis University, Budapest, Hungary P5A-203 The role of posttranslational modifications of microvesicles in systemic immune responses of mice

Katalin Szabó-Taylor 1,*Krisztina Pálóczi 1Tamás G. Szabó 1Andrea Németh 1Xabier

Osteikoetxea 1Barbara Sódar 1Marianna C. Holub 1Erna Pap 1Bence György 1Éva Pállinger

1Mária Pásztói 1Edit I. Buzás 1 1Dept . Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary P5A-204 Level of pro-coagulant microvesicles in-steady state-sickle cell anemia patients

Hassan A. Hamali 1,*Orwa Altayr 2Abdolmoneim Jamil 2Abdulrahman Alshehri 3 1Clinical Laboratory Sciecnes, King Khalid University, 2Pathology Department , College of Medicine, King Khalid University, 3Assir Central Hospital, Ministry of Health, Abha, Saudi Arabia P5A-205 Trophoblast-cell derived microparticles in women exposed to Plasmodium falciparum and HIV

Laura Moro 1,*Azucena Bardají 1 2Diana Barrios 1Gemma Moncunill 1Inácio Mandomando

2Betuel Sigaúque 2Carlota Dobaño 1 2Eusebio Macete 2Pedro L. Alonso 1 2Clara Menéndez 1

2Daniel Benitez 3Alfredo Mayor 1 2 1Barcelona Centre for International Health Research, Barcelona, Spain, 2Centro de Investigação em Saúde da Manhiça, Manhiça, Mozambique, 3Institut d'investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain


Poster Session 5C - EV in tissue repair & remodelling

Chair: TBA

Chair: Jess Morhayim

P5C-206

*Presenting author


Mesenchymal stem cell-derived exosomes containing microRNAs accelerate skeletal muscle regeneration

Yoshihiro Nakamura 1,*Shigeru Miyaki 1Hiroyuki Ishitobi 1Tomoyuki Nakasa 1Naosuke Kamei

1Mitsuo Ochi 1 1Hiroshima University, Hiroshima, Japan P5C-207 MSC-derived exosomes enhance myelination in cerebral hemisphere brain slices from neonatal rats.

Benjamin Buller 1,*Michael Chopp 1 2Mark Katakowski 1Wing Lee Cheung 3Zheng Gang Zhang

1 1Neurology, Henry Ford Health System, Detroit, 2Physics, Oakland Univeristy, Rochester, 3Emergency Medicine, Henry Ford Health System, Detroit, United States P5C-208 The potential role of iPSCs-derived microvesicles in delivery of bioactive content to target cells: implications for tissue regeneration

Marta Adamiak 1,*Sylwia Bobis-Wozowicz 1Zbigniew Madeja 1Ewa Zuba-Surma 1 1Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland P5C-209 Exosomes derived from human platelet lysate affect MSC functions in vitro

Elena Torreggiani 1,*Francesca Perut 1Laura Roncuzzi 1Nicola Baldini 1 2 1Laboratory for Orthopaedic Pathophysiology and Regenerative Medicine, Istituto Ortopedico Rizzoli, 2Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy P5C-210 Extracellular vesicles isolated from equine synovial fluid bind to and are internalized by chondrocytes and synoviocytes in vitro

Janneke Boere 1,*Chris H. van de Lest 2Esther N. Nolte-'t Hoen 2Sten F. Libregts 2Richard

Wubbolts 2Janny C. de Grauw 1Jos Malda 3Marca H. Wauben 2René P. van Weeren 1 1Equine Sciences, 2Biochemistry and Cell Biology, Utrecht University, Fac. Veterinary Medicine, 3Orthopaedics, University Medical Center Utrecht, Utrecht, Netherlands P5C-211 Abundance of active Lysyl Oxidase Like 2 on the surface of endothelial cell-derived exosomes is increased upon stimulation with hypoxia

Olivier De Jong 1,*Bas W.M. van Balkom 1Hendrik Gremmels 1Marianne C. Verhaar 1 1Nephrology and Hypertension, UMC Utrecht, Utrecht, Netherlands P5C-212 Remodeling and protecting the airways by epithelial exosomes like vesicles

*Presenting author


John T. Sheridan 1Sabri Abdulwahab 1Tiffany T. Wang 1Rui Cao 1Mehmet Kesimer 1,* 1University of North Carolina , Chapel Hill, United States P5C-213 Uptake of macrophage exosomes by the human placenta

Beth Holder 1,*Karen Forbes 2Sandra Okala 3Beate Kampmann 1 1Paediatrics, Imperial College, London, 2Maternal and Fetal Health Research, The University of Manchester, Manchester, 3Medicine, Imperial College, London, United Kingdom P5C-214 Extracellular vesicles from renal tubular cells induce an epithelial commitment of human bone marrow mesenchymal stromal cells

Stefania Bruno 1,*Giulia Chiabotto 2Federica Collino 2Giovanni Camussi 2 1Dep Biotechnology and Healthy Sciences, 2Medical Sciences, University of Torino, Torino, Italy P5C-215 Prostate cancer exosomes can differentiate mesenchymal stem cells into myofibroblast-like cells

Ridwana Chowdhury 1,*Jason Webber 1Zsuzsanna Tabi 1Malcolm Mason 1Aled Clayton 1 1Cardiff University, Institute of Cancer and Genetics, Cardiff, United Kingdom P5C-216 Exosomes derived from mesenchymal stem cells during osteogenic differentiation induce osteogenic differentiation of mesenchymal stem cells

Xiaoqin Wang 1,*Omar Omar 1Forugh Vazirisani 1Peter Thomsen 1Karin Ekström 1 1Department of Biomaterials, Sahlgrenska Academy at University of Gothenburg, and BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, Gothenburg, Sweden P5C-217 Characterisation of extracellular vesicles (EVs) from the secretome of human induced pluripotent stem cells (iPSCs) during the differentiation process into dendritic cells (DCs)

Yi Xin Fiona Lee 1,*Alison Leishman 2Patty Sachamitr 2Tim Davies 2Samir El Andaloussi 3Paul

Fairchild 2Matthew Wood 1 1Physiology, Anatomy and Genetics, 2Dunn School of Pathology, University of Oxford, Oxford, United Kingdom, 3Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden P5C-218 Extracellular Vesicles Secreted During Human Myoblast Differentiation Stimulate Skeletal Myogenic Differentiation of Human Adipose-Derived Stem Cells

Ji Suk Choi 1,*Kyoung Soo Lee 1Seong Hyun Yang 1Yong Woo Cho 1 1Chemical Engineering, Hanyang University, Ansan, Korea, Republic Of P5C-219

*Presenting author


Characterization of the extracellular vesicles released by C2C12 cells during the early phase of myogenic differentiation process

Michele Guescini 1,*Tibollo Pasquale 1Michela Battistelli 2Giosuè Annibalini 1Sara Salucci

2Luciana Vallorani 1Serena Maggio 1Lucia Casadei 1Elisabetta Falcieri 2Vilberto Stocchi 1 1Biomolecular Sciences, 2Dipartimento di Scienze della Terra, della Vita e dell'Ambiente (DiSTeVA), University of Urbino, Urbino, Italy P5C-220 Heterogeneity of skeletal muscle-derived extracellular nanovesicles and role of protein lipidation

Daniele P. Romancino 1Alessandra d'Azzo 2Mauro Manno 3Antonella Bongiovanni 4,* 1Institute of Biomedicine and Molecular Immunology (IBIM), National Research Council (C.N.R.), Palermo, Italy, 2genetics, st jude CRH, Memphis, United States, 3Institute of Biophysics (IBF), 4Institute of Biomedicine and Molecular Immunology (IBIM), National Research Council (CNR), Palermo, Italy P5C-221 Human plasma microparticles are specific source of pro- and antiangiogenic miRNAs

Ewa L. Stepien 1,*Iwona Wybrańska 1Roman Pfitzner 2Francisco J. Enguita 3 1Clinical Chemistry, 2Dept. Cardiac and Vascular Surgery and Transplantology, Institute of Cardiology, Collegium Medicum UJ, Krakow, Poland, 3Cell Biology Unit, Institute of Molecular Medicine, Institute of Molecular Medicine, University of Lisbon, Lisbon, Portugal P5C-222 Effects of different extracellular vesicles population on reversal of FDCP -1 cells radiation damage

Sicheng Wen 1,*Mark Dooner 1Mandy Pereira 1Arina Sorokina 1Elaine Papa 1Michael Del Tatto

1Yanhui Deng 1Laura Goldberg 1Jason Aliotta 1Peter Quesenberry 1 1Hematology/Oncology, Brown University/Rhode Island Hospital, Providence, United States


Poster Session 6C - EV as biomarkers

Chair: Rebecca Dragovic

Chair: Sammar Marei

P6C-223 Establishing the core proteome of human platelet exosomes using differential isolation techniques

Feidhlim Dervin 1,*Kieran Wynne 1Tiina O'Neill 1Patricia B. Maguire 1 1School of Biomolecular and Biomedical Research, University College Dublin, Conway Institute, Dublin, Ireland P6C-224

*Presenting author


Characterization and quantification of circulating microparticles in patients with Diamond Blackfan anemia (DBA)

Rossella Crescitelli 1 2,*Serena Macrì 1Elisa Pavesi 1Anna Aspesi 1Claudia Vizziello 3Patrizia

Notari 3Carlotta Botto 4Giorgio Bellomo 3Jan Lötvall 2Ugo Ramenghi 4Irma Dianzani 1 1Department of Health Sciences, University of Eastern Piedmont, Novara, Italy, 2Department of Internal Medicine, Krefting Research Centre, University of Gothenburg, Gothenburg, Sweden, 3Chemical Clinical Analysis laboratory, SCDU, Azienda Universitaria Ospedaliera Maggiore della Carità, Novara, 4Department of Pediatric and Public Health, University of Turin, Torino, Italy P6C-225 Urinary exosomes for the diagnosis of Gitelman and Bartter syndrome

Marina Pitto 1,*SAMUELE CORBETTA 1ALBERTO BETTINELLI 2FRANCESCA RAIMONDO 1 1DEPT HEALTH SCIENCES, UNIVERSITY MILANO-BICOCCA, MONZA, 2Dept. of Pediatrics, San Leopoldo Mandic Hospital, MERATE, Italy P6C-226 Identification of circulating exosomes as biomarkers for drug-induced liver injury

Young-Eun Cho 1,*Pyong-Gon Moon 1Jeong-Eun Lee 1Moon-Chang Baek 1 1Molecular Medicine, Kyungpook National University, Daegu, Korea, Republic Of


Poster Session 10B - Late Breaking

Chair: TBA

Chair: TBA


Poster Session 11 - EV in reproduction & pregnancy

Chair: Rebecca Dragovic

Chair: Sammar Marei

P11-227 Galectins as major components in amniotic fluid derived exosomes

Marei Sammar 1,*Niko Bretz 2John Hazin 2Peter Altevogt 2 1Prof. Ephraim Katzir department of Biotechnology Engineering, ORT Braude College, Karmiel, Israel, 2Tumor Immunology Programme, D015, German Cancer Research Center, Heidelberg, Germany P11-228 Glucocerebrosidase and extracellular vesicle shedding from a placental cellline

Hajar Hassani Lahsinoui 1,*Remco Keijser 2Zargun Roghbar 2Gijs B. Afink 2Carrie Ris-Stalpers 1 1Department of Obstetrics and Gynaecology, 2Reproductive Biology Laboratory, Academisch medisch centrum , Amsterdam, Netherlands

*Presenting author


P11-229 Glycan profile of amniotic fluid extracellular vesicles

Maja Kosanović 1,*Ljiljana Gavrilović 1Ivana Buzadžić 2Ivana Grubiša 2Ninoslav Mitić 1Bojana

Milutinović 1Miroslava Janković 1 1Department of Immunochemistry and Glycobiology, University of Belgrade, Institute for the Application of Nuclear Energy, INEP, 2Department of human genetics and prenatal diagnostics, Clinical Hospital Center Zvezdara, Belgrade, Serbia P11-230 Deep sequencing analysis of prostasomal RNA

Carla Zijlstra 1,*Marian Aalberts 1Nico Lansu 2Ewart de Bruijn 2Peter-Bram 't Hoen 3Henk B.

Buermans 3Esther N. Nolte-'t Hoen 1Tom A. Stout 4Marca H. Wauben 1Willem Stoorvogel 1 1Biochemistry and Cell Biology, Universiteit Utrecht, 2Hubrecht Institute, Utrecht, 3Center for Human and Clinical Genetics, Leiden University Medical Center, Leiden, 4Equine Sciences, Universiteit Utrecht, Utrecht, Netherlands P11-231 Effect of extracellular vesicles secreted by bovine oviductal epithelial cells in in vitro bovine embryo production R Lopera 1M Hamdi 1B Fuertes 1V Maíllo 1P Beltran 1A Redruello 2A Gutierrez-Adan 1Maria

Yáñez-Mó 2,*MA Ramírez 1D Rizos 1 1INIA, 2Unidad de Investigación, Hospital Santa Cristina, Instituto de Investigación Sanitaria Princesa (IIS-IP/FIB-HUP), Madrid, Spain P11-232 Improvement of cloned embryos development via co-culturing with parthenotes: a possible role of exosomes/microvesicles for embryos paracrine communication

Islam Saadeldin 1,*Su Jin Kim 1Yoo Bin Choi 1Byeong Chun Lee 1 1Theriogenology and Biotechnology, Seoul National University, Seoul, Korea, Republic Of

Documents

Thursday May 1, 2014 Oral Presentations - MCI Groupimgpublic.mci-group.com/nl/ISEV/Abstracts/Thursday_May_1.pdf · Chair: Steve Gould Chair: ... Thursday May 1, 2014 ... of Molecular