TisXell Regeneration System

Company Background…(1) QuinXell Technologies Pte Ltd was founded

in 2011

QuinXell is the latest addition to Quintech Life Sciences (QLS) family of companies established to tap into the emerging Life Science industry

QuinXell will focus in the area of Regenerative Medicine through its innovative cell culture-based technologies

Company Background…(2) Research on QuinXell’s TisXell Regeneration

System was initiated as a joint project between the National University of Singapore (NUS) and Singapore Polytechnic (SP), with the National University Hospital (NUH) providing samples for applications

Scientific papers supporting the benefits of biaxial bioreactor on functional tissue growth (tissue engineered constructs or TECs) on 3D scaffolds have been published in international journals for more than 10 years

Background Information…(1)

Regenerative Medicine has been hailed as a revolutionary medical advancement, breathing new life into patient care

Transplantation through in vitro cultures of organs and tissues or Tissue Engineering (TE) is a standardized and reproducible method to create functional tissues in the laboratory

These tissues are utilized to replace, repair or regenerate organs loss due to disease, trauma, failure or cogenital defects

Background Information …(2)

Traditionally, spinner flasks, rotating wall vessels (RWV), perfusion and uniaxial bioreactors are employed for TE

QuinXell’s unique TisXell Regeneration System offers an optimal condition for high growth and high proliferation of cell cultures compared to conventional methods.

TisXell controlled environment provides optimal nutrients and gases to growing cells and triggers cellular mechano-transduction signalling pathways to stimulate tissue remodelling onto a 3D scaffold.

Application FieldsFor research institutes, hospitals,

biotechnology, pharma in• Regenerative medicine

• Tissue engineering

• Translational medicine

• Tissue and organ transplant

Cell Types• Primary Cells

• Stem Cells

• Bone Cells

• Muscles Cells

• And many more…

Conventional MethodsTypes of Bioreactors Advantages Disadvantages

Spinner flasks Improved fluid flow (as compared to static vessels)

Turbulence generated can be detrimental for seeded cells and newly laid down ECM

Perfusion bioreactors Enhances mesenchymal stem cell proliferation and osteogenic differentiation in scaffold constructs

Non-homgenous cellular distributionCells at frontal zones are washed away by the oncoming perfusion flow with higher flow rates

Rotating wall vessel (RWV) bioreactors

Low sheer forces and 3D high mass transfer (similar to perfusion bioreactors)

Non-homogenous cellular distribution and ECM depositionIn the free floating culture, collision between scaffolds and bioreactor walls may induce cellular damage and disrupt cellular attachment and matrix deposition on the scaffolds

TisXell Novel ApproachTypes of Bioreactors Advantages

Biaxial revolving bioreactore.g. TisXell

Integrated perfusion system allows for exchange between the vessel and reservoir with maximal mass transfer under low sheer force

Biaxial revolution of the culture vessel led to more homogenous cellular and ECM deposition – at the surface and the deeper core of the scaffolds

Cellular scaffolds were secured by pins to avoid scaffold and vessel wall collisions

TisXell Regeneration System

TisXell – Unique Selling Points…(1)

Patented biaxial revolution in two independent orthogonal axes (spin and tumble simultaneously)

Flexible operational modes available – biaxial, uniaxial, swing modes

TisXell – Unique Selling Points…(2)

Efficient fluid transport within the 3D scaffolds, allowing optimal nutrients and waste exchange to and from the cells, penetrating the deepest core of the scaffolds

Spherical design of vessel reduces drag and promotes uniform fluid mixing

TisXell – Key Benefits…(1)

Accelerates cell growth, differentiation and cell proliferation, mimicking native extracellular matrix (ECM)

Supports homogenous cell culture at the surface and core of the 3D scaffolds

Maintains functionality and viability of tissue constructs

TisXell – Key Benefits…(2)

Reduces formation of necrotic neotissue that interferes with subsequent implantation process

Promotes integration of implants with surrounding tissue and support the structural integrity in regeneration of tissues and/or organs

Key Benefits for Bone Applications…(1)

In Vitro Homogenous cellular distribution

Rapid stem cell expansion – up to 2-fold (or more) improvement in mass transfer in thicker grafts

High viability

Robust osteogenic differentiation and mineralization

Key Benefits for Bone Applications…(2)

In Vivo Increased bone formation after

transplantation

Promotes new vascularization

TisXell System Configuration ITisXell Regeneration System, BasicIncludeso Two spherical glass vessels of 500ml and 1000ml, 1

eacho Revolving stand to hold one vessel, 1o Integrated peristaltic pump, 1o Medium reservoir, 1o Remote touch screen panel, 1o Tubings and connectors starter kit, 1o Scaffolds starter kit, 1

TisXell System Configuration IITisXell Regeneration System, CompleteIncludeso Two spherical glass vessels of 500ml and 1000ml, 1

eacho Revolving stand to hold one vessel, 1o Integrated peristaltic pump, 1o Medium reservoir, 1o Remote touch screen panel, 1o Tubings and connectors starter kit, 1o Scaffolds starter kit, 1o Set of probes, 1o Applikon console and monitor, 1