7/24/2019 TM DNA

1/5

Inciso a)

GGTATGATAGTACCTACATATATCTATATCCCGGATTTAA

GGTATGATAGTACCTACATATATCCCCGGTTCGCCCCTAT

GGTATGATAGTACCTACATATATCTATTTATGGCGGCTCC

7/24/2019 TM DNA

2/5

Inciso b)

GCATAGCTCATGATGCTCGATACGTCGAGTACAGCTGCAT

CAGTATACTGACTCGTAGCAGATGCGCATTAGCATGCCGT

ATGCGCATTAGCATGCCGTACGTATACGCGATGACTCGTA

7/24/2019 TM DNA

3/5

Inciso c)

Oligo: 5'-

TAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGC

-3'

Primer1: 60 bases

Composition 15 A 15 C 15 G 15 T 0 OT!"#

Percentage: $5% A $5% C $5% G $5% T 0%OT!"#

&(1*5$ +,a

7/24/2019 TM DNA

4/5

!bri.i/ation: ,:,

alt: 50 m&

ormami.e: 0%

&ismatc2: 0 bp

T2ermo Tm ( 5*4 C %GC Tm ( $*1 C GCAT Tm ( 10*0C

Oligo: 5'-TAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGC-3'

Primer1: 3$ bases

Composition A C G T 0 OT!"#

Percentage: $5% A $5% C $5% G $5% T 0%OT!"#

&(7* +,a

!bri.i/ation: ,:,

alt: 50 m&

ormami.e: 0%

&ismatc2: 0 bp

T2ermo Tm ( 63* C %GC Tm ( 64* C GCAT Tm ( 76*0 C

Oligo: 5'-TAGCTAGCTAGCTAGCTAGC-3'

Primer1: $0 bases

Composition 5 A 5 C 5 G 5 T 0 OT!"#

Percentage: $5% A $5% C $5% G $5% T 0%OT!"#

&(6*1 +,a

!bri.i/ation: ,:,

alt: 50 m&

ormami.e: 0%

&ismatc2: 0 bp

7/24/2019 TM DNA

5/5

T2ermo Tm ( 47*6 C %GC Tm ( 55*4 C GCAT Tm ( 60*0 C