To Question of Creating of Experimental Models, Coded by Abstract Rlung, Mkhris, Badgan Thermins in Traditional Medicine

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To question of creating of experimental models, coded by abstract rlung, mkhris,

badgan thermins in Traditional medicine

M.Ambaga, A.Tumen-Olzii (Mongolia, New medicine /NCM/-medical institute,Ulanbaatar)

Introduction.

Previously, we confirmed that the appearing of abstract theory of Rlung,Mkhris,Badgan

in Tradititional medicine 2 thousand years ago follow from the functioning of membrane-redoxy

potentials three state line systems in the first compartment with close relationship with fourth

and second, third compartments in human body.

We also reveal that the functional and structural unit of living cells,as membrane-redoxy

potentials three state line systems in the first compartment with close relationship with fourth

and second, third compartments in human body reflected in the theory of Tibetian- Mongolian

Traditional medicine in the following philosophical context as wind- like mobile,unoily,light

nature-external characteristics of human body,symbolized by rlung abstract thermin, sun-like

hot,hot oily nature-external characteristics of human body, symbolized by mkhris abstract

thermin, moon-like cool, heavy, cold oily nature-external characteristics of human body,

symbolized by badgan abstract thermin.

To confirm the our conception as that the appearing of abstract theory of

Rlung,Mkhris,Badgan in Tradititional medicine 2 thousand years ago- follow from the

functioning of membrane-redoxy potentials three state line systems in the first compartment with

close relationship with fourth and second, third compartments in human body we are

conducting the our study in experimental animals,during which planned observe

following relationship as :a.The association between alteration in the level of f luid alpha states,consisting of

unsaturated fatty acids with high levels of oxy potentials and with high levels of

proton,electrons conductance and high levels heat energy release andsun-like hot,hot oily

nature-external characteristics ,symbolized by mkhris abstract thermin.

b. The association between alteration in the level of solid betta state,consisting of

mainly saturated fatty acids, conditioning a high levels of red potentials and with slow levels

of proton,electrons conductance and low levels of heat energy release, high degree of energy

accumulation and high degree of high energy phosphate-ATP, high energy electrons NADPH

and moon-like cool, heavy, cold oily nature-external characteristics,symbolized by

badgan abstract thermin.

c. The association between alteration in the level of gamma state, consisting of

decreased contents of saturated-unsaturated fatty acids, conditioning a decreased

levels of redoxy potentials and with slow levels of proton, electrons conductance and low

levels of heat energy release and energy accumulation and low degree of high energy

phosphate-ATP, high energy electrons NADPH and wind- like mobile, unoily, light nature-

external characteristics, symbolized by rlung abstract thermin



Experimental protocols.

Basic methods of investigation.

Experimental justification of basic conception of NCM medicine are conducted in followinfg

steps.

Subjects of our experimental works was rabbits aged 18-24 months, including male andfemale, ratio 50:50.

A.Experimental inducing of f luid alpha states,consisting of unsaturated fatty acids withhigh levels of oxy potentials and with high levels of proton,electrons conductance and highlevels heat energy release in a rabbits was caused by injection of Dinitrophenol 2-4 (3,5 мg/ kg,four days,under cutaneous ) .

Dinitrophenol 2-4 - mechanism of action is by inhibition of the F0-F1 ATP synthase molecule, located in the inner membranes of each mitochondrion. The electrontransport chain still functions to pump hydrogen ions into the intermembrane space,

but the coupling of the proton gradient to ATP production is rendered impossible byDNP. As a result, ATP production is dramatically reduced, and the energy is insteadthrown off as heat... DNP is lipophilic weak acids that therefore readily pass throughmembranes,they bind protons in the acidific side of the membrane-intermembranespaces ,diffuse through , and release them on the alkaline side-matrix side ,therebydissipating the proton gradient metabolic rate is increased..(D.Voet,Biochemistry,p.553).

Effects of 2,4-dinitrophenol on lipoxygenase activity and fatty acid constituents of membrane lipids in pericarp of harvested longan fruits.Chen Lian; Lin HeTong; Chen

YiHui; Lin YiFen; Chen ShaoJun нарын Journal of Tropical and Subtropical Botany 2009Vol. 17 No. 5 pp. 477-482 –:

The effects of 2,4-dinitrophenol (DNP, a uncouple agent for respiratory) on lipoxygenase (LOX)

activity, fatty acid constituents in membrane lipids and cellular membrane permeability in

harvested longan (Dimocarpus longan Lour. 'Fuyan') pericarp were investigated and the

relationship to pericarp browning was analysed. The results showed that the cellular membrane

permeability, LOX activity and browning index increased treated with DNP, the saturated fatty

acids, such as palmitic acid (C16:0) and stearic acid (C18:0) increased, and the unsaturated fatty

acids, such as linoleic acid (C18:2), linolenic acid (C18:3) and (C20:1), and index of unsaturated fatty

acids (IUFA) and unsaturation degree of fatty acids decreased. It suggested that the pericarp

browning of longan induced by DNP might be due to increasing LOX activity, enhancing

degradation of membrane unsaturated fatty acid and reducing integrity of cellular membrane,

which it lead to finally the contact of phenolase with phenolic substrates to produce browning

polymers.

5-Lipoxygenase (5-LO) catalyzes two steps in biosynthesis of leukotrienes (LTs), a

group of lipid mediators of inflammation derived from arachidonic acid (AA). LT

antagonists are used in treatment of asthma; more recently a potential role also in

atherosclerosis has raised considerable interest. Furthermore, possible effects of 5-LO

metabolites in relation to tumorigenesis have emerged. Thus, an understanding of the



biochemistry of this lipoxygenase has potential implications for treatment of various

diseases.

Leukotrienes (LTs) are inflammatory mediators causing, for example, phagocyte

chemotaxis and increased vascular permeability In leukotriene biosynthesis 5-

lipoxygenase (5-LO) catalyzes oxygenation of arachidonic acid (AA) to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid (5-HPETE), and further

dehydration to the allylic epoxide leukotriene A4 (1). As one of six human

lipoxygenases, 5-LO is expressed primarily in various leukocytes: polymorphonuclear

leukocytes (neutrophils and eosinophils), monocytes/macrophages, mast cells, B-

lymphocytes, dendritic cells, and foam cells of human atherosclerotic tissue. LTA4 is

further converted by LTA4 hydrolase to the dihydroxyacid LTB4, and by LTC4 synthases

to the glutathione conjugate LTC4. The other cysteinyl-LTs are formed by hydrolytic

removal of γ-Glu and Gly from LTC4 (yielding LTD4 and LTE4). In proinflammatory

contexts, LTs typically stimulate cellular responses, which are quick in onset and of

short duration (as smooth muscle contraction, phagocyte chemotaxis, increased

vascular permeability). These are mediated via G-protein coupled receptors, BLT1/2 for

LTB4 and CysLT1/2 and GPR17 for the cys-LTs.

B.Experimental inducing of solid betta state,consisting of mainly saturated fatty acids,conditioning a high levels of red potentials and with slow levels of proton,electronsconductance and low levels of heat energy release, high degree of energy accumulation andhigh degree of high energy phosphate-ATP, high energy electrons in a rabbits was caused byinjection of Rotenon (2,5 мg/ kg, four days,under cutaneous ).

Rotenone blocks cytochrome oxidase (complex 4) and prevents both coupled and uncoupled

respiration with all substrates, including NADH, succinate and ascorbate + TMPD.

Rotenone inhibits the oxidation of NADH to NAD, blocking the oxidation by NAD of substrates

such as glutamate, alpha-ketoglutarate, and pyruvate. Rotenone inhibits the mitochondrial

respiratory chain between diphosphopyridine nucleotide and flavine. Rotenone is a powerful

inhibitor of mitochondrial electron transport (Goodman, 1985).

First step:

Take from all animals a blood example and separate erythrocyte populations of cells.

Second step:

Cell materialls are divided in 3 basic parts,consisting first compartment and second

compartment, also fourth compartmemt.

First compartment (hemolysate) of erythrocytes are obtained by causing hemolysis with

following centrifuging, separating fluid upper part of supernatant,containing redoxy line systems.

After separating hemolysate of erythrocyte all membrane systems, representing fourth

compartment also are taken by washing and centifuging remnant parts.

Third step:



To identification of basic membrane states and redoxy potentials of hemolysate,representing

first compartment, containing redoxy line systems are used following parameters:

a.Reducing capacity of of hemolysate, established by using the nitroblue tetrazolium test

b.The protonizing, radical scavenging activity of hemolysate , evaluated against 2,2-

diphenyl-1-picrylhydrazyl radical. The protonizing, radical scavenging activity expressed

as IC50 . c.Contents of Deprotonized products of hemolysate ,accounted by concentration of TBA

positive products.

d.Oxidizing capacity of of hemolysate by using the oxidase disk test

Fourth step:

To identification of basic membrane states and redoxy potentials of all membrane

system,representing fourth compartmemt are used following parameters:

a.Reducing capacity of all membrane system,representing fourth compartmemt by using the

nitroblue tetrazolium test

b. The protonizing, radical scavenging activity of all membrane system,presenting fourthcompartmemt hemolysate, evaluated against 2,2-diphenyl-1-picrylhydrazyl radical. The

protonizing, radical scavenging activity expressed as IC50 .

c.Oxidizing capacity of all membrane system, presenting fourth compartmemtby using the

oxidase disk test

d.Contents of Deprotonized products of all membrane system, representing fourth

compartmemt determined by concentration of TBA positive products.

e. Proportion of saturated and unsaturated fatty acids contained in LPHD and LPLD,

incorporated in “ integrated membrane-serum circultion system”i.e. constantly circulated

between all cell membrane structures and serum (second compartment ) medium are

measured by using Humalyser apparatus .

f. Degree of saturated and unsaturated fatty acids dependant resistanse of all membrane

system, presenting fourth compartmemt are evaluated by using osmotic and pereoxidation

hemolysis test.

Fifth step:

a. Redoxy potentials of second compartment of animals,calculated by parameters of AO:LPO

system, contents of LPHD and LPLD

b. Reducing capacity of second compartment of animals, established using the nitroblue

tetrazolium test.

c. The protonizing, radical scavenging activity of of second compartment of animals,

evaluated against 2,2-diphenyl-1-picrylhydrazyl radical. The protonizing, radical

scavenging activity expressed as IC50. d. Contents of Deprotonized products of second compartment of animals, accounted by

concentration of TBA positive products.

e. Contents of cholesterols, triglycerids and LPHD, LPLD.

f. Oxidizing capacity of second compartment of animals, established by using the oxidase disk

test



Results of investigation

First results:

A.Change of oxidizing activity of hemaglobine molecules in the first compartment

during of modelling of high alpha state-high oxy potential elevated situation in rabbits

by dinitrophenol (dinitrophenolmediated “proton loss process ”) and their relationship

with abstract Mkhris code.We revealed that in the case of modelling of high alpha state-high oxy potential elevated

situation in rabbits by dinitrophenol (dinitrophenolmediated “proton loss process ”) are

increased the oxidizing activity of hemaglobine molecules in 3,0-7,67 times in comparision with

control animals after 3, 7, 14 days of modelling, which is on of appearanse of a sun like hot, hot

oil external characteristics, coded by abstract mkhris thermin.

B.Change of reduction :oxidation ratio activity of second compartment (serum oxidase

enzyme activity) during of modelling of membrane high alpha state-high oxy potential

elevated situation in rabbits by dinitrophenol (dinitrophenolmediated “proton loss

process”) and their relationship with abstract Mkhris code.

By us established that in the case of modelling of high alpha state-high oxy potentialelevated situation in rabbits bydinitrophenol (dinitrophenol mediated “proton loss process”)

are increased the reduction :oxidation ratio activity of second compartment (serum

oxidase enzyme activity) in 1,2-1,6 times in comparision with control animals after 3,7,14 days

of modelling,which is on of appearanse of a sun like hot,hot oil external characteristics ,coded

by abstract mkhris thermin.

C.Change of the free radical protonizing activity of membrane-redoxy 3 state line

systems, existing in the first compartment) during of modelling of membrane high alpha

state-high oxy potential elevated situation in rabbits by dinitrophenol

(dinitrophenolmediated “proton loss process”) and their relationship with abstract

Mkhris code.

It was clear that in the case of modelling of high alpha state-high oxy potential elevated

situation in rabbits by dinitrophenol (dinitrophenolmediated “proton loss process ”) are slowed

the free radical protonizing activity of membrane-redoxy 3 state line systems ,existing in the first

compartment) in 1,1-1,12 times in comparision with control animals after 3, 7, 14 days of

modelling,which is on of appearanse of a sun like hot, hot oil external characteristics ,coded by

abstract mkhris thermin.

D.Change of the quantity of deprotonized products in the second compartment during of

modelling of membrane high alpha state-high oxy potential elevated situation in rabbits

by dinitrophenol (dinitrophenolmediated “proton loss process”) and their relationship

with abstract Mkhris code.

We revealed that in the case of modelling of high alpha state-high oxy potential elevated

situation in rabbits by dinitrophenol (dinitrophenolmediated “proton loss process”) are increased

the quantity of deprotonized products in the second compartment in 1,2 times in comparision



with control animals after 3, 7, 14 days of modelling,which is on of appearance of a sun like

hot, hot oil external characteristics, coded by abstract mkhris thermin.

E.Change of the quantity of deprotonized products in the fourth compartment during of



with abstract Mkhris code.

It was clear that in the case of modelling of high alpha state-high oxy potential elevated


increased the quantity of deprotonized products in the fourth compartment in 1,2 times in

comparision with control animals after 3,7,14 days of modelling,which is on of appearanse of a

sun like hot,hot oil external characteristics ,coded by abstract mkhris thermin.

I.Change of the quantity of deprotonized products in the fourth compartment during of



with abstract Mkhris code.By us revealed that in the case of modelling of high alpha state-high oxy potential

elevated situation in rabbits by dinitrophenol (dinitrophenolmediated “proton loss process ”)

are increased the resistance of membrane structures ,belonging tofourth compartment in 1,18

times in comparision with control animals after 3,7,14 days of modelling,which is on of

appearanse of a sun like hot,hot oil external characteristics ,coded by abstract mkhris thermin.

Second results:

A. Change of oxidizing activity of hemaglobine molecules in the first

compartment during of modelling of membrane high betta state-high reduction

potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in

the complex 4 with elevation of reduced form of NADH ) and their relationship with

abstract Badgan code.

We revealed hat in the case of modelling of high solid betta state mediated highreduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidasein the complex 4 with elevation of reduced form of NADH ) are decreased the oxidizingactivity of hemaglobine molecules in 1,8-14,0 times in comparision with control animals after 3,7,14 days of modelling,which is on of appearance of a moon like cold, cool oil external

characteristics ,coded by abstract badgan thermin.

B. Change of oxidizing activity of hemaglobine molecules in the first







By us established that in the case of modelling of high solid betta state mediated highreduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidasein the complex 4 with elevation of reduced form of NADH ) are decreased the oxidizingactivity of hemaglobine molecules in 1,8-14,0 times in comparision with control animals after 3,7,14 days of modelling,which is on of appearance of a moon like cold, cool oil externalcharacteristics ,coded by abstract badgan thermin.

C.Change of reduction :oxidation ratio activity of second compartment (serum

oxidase enzyme activity )during modelling of membrane high betta state-high reduction




It was clear that in the case of modelling of membrane high betta state-high reduction

potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in the

complex 4 with elevation of reduced form of NADH ) are decreased the reduction :oxidation

ratio activity of second compartment (serum oxidase enzyme activity ) in 1,6-7,25 times in

comparision with control animals after 3,7,14 days of modelling,which is on of appearance of a

moon like cold, cool oil external characteristics ,coded by abstract badgan thermin.

D. Change of oxidizing activity of hemaglobine molecules in the second





We observed that in the case of modelling of high solid betta state mediated highreduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidasein the complex 4 with elevation of reduced form of NADH ) are decreased the thedeprotonized products in the second compartment in 1,2 times in comparision with controlanimals after 3,7,14 days of modelling,which is on of appearance of a moon like cold, cool oilexternal characteristics ,coded by abstract badgan thermin.

E. Change of deprotonized products in the forth compartment during of

modelling of membrane high betta state-high reduction potential elevated situation in

rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of

reduced form of NADH ) and their relationship with abstract Badgan code.

By us established that in the case of modelling of high solid betta state mediated high

reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase

in the complex 4 with elevation of reduced form of NADH ) are decreasedthe deprotonized

products in the forth compartment in 1,3 times in comparision with control animals after

3,7,14 days of modelling,which is on of appearance of a moon like cold, cool oil external


I. Change of the free radical protonized activity of membrane-redoxy 3 state line

systems in the first compartment and membrane structures in the forth compartment

during of modelling of membrane high betta state-high reduction potential elevated



situation in rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with

elevation of reduced form of NADH ) and their relationship with abstract Badgan code.

We observed that in the case of modelling of high solid betta state mediated high

reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase

in the complex 4 with elevation of reduced form of NADH ) are increased the free radical

protonized activity of membrane-redoxy 3 state line systems in the first compartment andmembrane structures in the forth compartment in 1,3 times in comparision with control

animals after 3,7,14 days of modelling,which is on of appearance of a moon like cold, cool oil

external characteristics ,coded by abstract badgan thermin.

L. Change of the resistance of membrane structures ,belonging to the forth





It was clear that in the case of modelling of high solid betta state mediated high

reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidasein the complex 4 with elevation of reduced form of NADH ) are increased the resistance of

membrane structures ,belonging to the forth compartment in 1,2 times in comparision with

control animals after 3,7,14 days of modelling,which is on of appearance of a moon like cold,

cool oil external characteristics ,coded by abstract badgan thermin.

M. Change of some parameters in the all 4 compartments during of modelling of

membrane high gamma state-slow redoxy potential situation in rabbits long time use

of rotenon (by blocking cytochrome oxidase in the complex 4 with elevation of reduced

form of NADH ) and their relationship with abstract Badgan code.

By us established that in the case of modelling of high solid betta state mediated highreduction potential elevated situation in rabbits by long time use of rotenone(by blocking

cytochrome oxidase in the complex 4 with elevation of reduced form of NADH )

rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of

NADH ) are increased the contens of LPLD,cholesterols,triglycerids i.e.decline of dilution

effect of LPHD in relation to LPLD,cholesterols,triglycerids and decreased the contens of

LPHD in the the second compartment in 1,2 times in comparision with control animals after

28 days of modelling,which is on of appearance of a moon like cold, cool oil external


Third results:

A. Change of some parameters in the all 4 compartments during of modelling of membrane high gamma state-slow redoxy potential situation in rabbits long time use

of dinitrophenol (dinitrophenolmediated “proton loss process ”) and their relationship

with abstract Rlung code.

We established that in the case of modelling of membrane high gamma state-slow

redoxy potential situation in rabbits long time use of dinitrophenol (dinitrophenolmediated

“proton loss process ”) are decreased the ATP concentration by uncoupling process,because of



this decreased the ATP-ase activity ,leading to decline of slip redox potential , evidence of

these was the decrease of glucose concentration in the second compartment,decline of

concentration of LPLD , LPHD in 1,6 times in comparision with control animals after 14

days of modelling,which is on of appearance of a wind like unoily, mobile ,light external

characteristics ,coded by abstract rlung thermin.

Tab №1. Change of the intensity of NADPH generation during of modelling of

membrane high betta state-high reduction potential elevated situation and during of


(after three days of modelling).

№ Study groups Content of NADPH in the firstcompartment

1 Control animals 2.661+_0,014

2 Dinitrophenol administrated animals 2.407+_0,013

3 Rotenon administrated animals 2.755+_0,017

Tab №3. Change of the oxidizing activity of hemaglobine molecules during of

modelling of membrane high betta state-high reduction potential elevated situation

and during of modelling of membrane high alpha state-high oxy potential elevated

situation in rabbits (after three days of modelling).

№ Study groups The oxidizing activity of hemaglobine molecules in thefirst compartment (minute )

1 Control animals 14.75+_0,0242 Dinitrophenol administrated animals 7.67+_0,018


Tab №4. Change of the reduction oxidation ratio(serum oxidase enzyme activity)

in the second compartment during of modelling of membrane high betta state-high

reduction potential elevated situation and during of modelling of membrane high

alpha state-high oxy potential elevated situation in rabbits (after three days of

modelling).

№ Study groups The reduction oxidationratio(serum oxidase enzyme

activity) in the secondcompartment (minutes )

1 Control animals 4.25+_0,0242 Dinitrophenol administrated animals 2,0+_0,014


Tab №6. Change of the protonizing activity of membrane-redoxy 3 state line

system during of modelling of membrane high betta state-high reduction potential



elevated situation and during of modelling of membrane high alpha state-high oxy

potential elevated situation in rabbits (after three days of modelling ).

№ Study groups Abs IC50

1 Control animals 0.097 77.65

2 Dinitrophenol administrated animals 0.087 79.95

3 Rotenon administrated animals 0.095 78.11

Tab №7. Change of free radical protonizing activity of membrane-redoxy 3 state

line system ,located in the first compartment during modelling of membrane high betta

state-high reduction potential elevated situation and during of modelling of membrane

high alpha state-high oxy potential elevated situation in rabbits (after three days of

modelling).

№ Study groups Abs IC50

1 Control animals 0.197 54.61

2 Dinitrophenol administrated animals 0.204 52.993 Rotenon administrated animals 0.197 54.61

Tab №8. Change of contents of deprotonized products in the second

compartment (serum TBA-positive products )during modelling of membrane high betta

state-high reduction potential elevated situation and during of modelling of membrane

high alpha state-high oxy potential elevated situation in rabbits (after three days of

modelling).

№ Study groups The contents of deprotonized products

in the second

compartment1 Control animals 0.058+_0,0014


Tab №9. Change of contents of deprotonized products in 5 membrane

structures,belonging to the fourth compartment (serum TBA-positive products )during



situation in rabbits (after three days of modelling ).

№ Study groups The contents of deprotonizedproducts in 5 membrane

structures,belonging to the fourthcompartment (serum TBA-

positive products )






Tab №10. Change of contents of deprotonized products in 5 membrane

structures,belonging to the fourth compartment (serum TBA-positive products )during



situation in rabbits (after three days of modelling ).

№ Study groups The contents of deprotonized products in5 membrane structures,belonging to the

fourth compartment (serum TBA-positiveproducts )




Tab №11. Change of the resistance of 5 membrane structures,belonging to the

fourth compartment during modelling of membrane high betta state-high reduction

potential elevated situation and during of modelling of membrane high alpha state-

high oxy potential elevated situation in rabbits (after three days of modelling ).

№ Study groups The resistance of 5 membranestructures,belonging to the

fourth compartment




Tab №12. Change of the antipereoxidation resistance of 5 membrane

structures,belonging to the fourth compartment during modelling of membrane high

betta state-high reduction potential elevated situation and during of modelling of

membrane high alpha state-high oxy potential elevated situation in rabbits (after three

days of modelling).

№ Study groups The antipereoxidation resistanceof 5 membranestructures,belonging to the fourth

compartment 1 Control animals 1.830+_0,093



Tab №13. Change of the osmotic resistance of 5 membrane structures,belonging to

the fourth compartment during modelling of membrane high betta state-high



reduction potential elevated situation and during of modelling of membrane high

alpha state-high oxy potential elevated situation in rabbits (after three days of

modelling).

№ Study groups The osmotic resistance of 5membranestructures,belonging to thefourth compartment




Tab №14. Change of contents of cholesterols in the second compartment during

modelling of membrane high betta state-high reduction potential elevated situation and

during of modelling of membrane high alpha state-high oxy potential elevated situation

in rabbits (after three days of modelling).

№ Study groups The contents of cholesterolsin the second compartment




Tab №16. Change of contents of LPHD in the second compartment during modelling

of membrane high betta state-high reduction potential elevated situation and during

of modelling of membrane high alpha state-high oxy potential elevated situation in

rabbits (after three days of modelling ).

№ Study groups The contents of LPHD in the second

compartment




Tab №17. Change of contents of LPLD in the second compartment during modelling

of membrane high betta state-high reduction potential elevated situation and during

of modelling of membrane high alpha state-high oxy potential elevated situation inrabbits (after three days of modelling ).



№ Study groups The contents of LPLD in thesecond compartment




Parameters of body weight and body temperature.

Days of observation

Parameters of observation

2 day 3days

4days

5days

6days

16days

22days

The bodyweight

Control animals 1.687 1.687 1.687 1.825 1.86 1.98 1.74

Dinitrophenoladministrated animals

1.675 1.675 1.615 1.60 1.58 1.88 1.68

Rotenon administratedanimals

1.725 1.775 1.837 1.90 1.90 1.97 1.65

The bodytemperature

Control animals 37.18 37.43 37.83 37.0 37.35 37.68 37.2

Dinitrophenoladministrated animals

38.6 38.2 38.25 38.4 38.5 37.07 36.73

Rotenon administratedanimals

38.6 37.23 37.13 36.75 36.6 36.6 36.2

Discussion.

1.We observed that in the case of modelling of high alpha state-high oxy potential elevated


provocated the increase of body temperature ,decrease of reduced form of NADPH contents,

increase of oxidized form of NADP contents ,oxidizing activity of hemaglobine molecules ,decrease of reduction :oxidation ratio in a first and fourth compartments ,also reduction activity

of membrane-redoxy potentials three state line systems,decrease of membrane resistance of

membrane structures of 1 compartment ,elevation of quantity of LPHD in second compartment,

increase of glucose utilization speed in second compartment with following decrease of glucose

concentration in a second compartment, increase of oxidized- deprotonized in a 5 membrane

structures,belonging to fourth compartment ,exhibiting a sun like hot,hot oil external

characteristics ,coded by abstract mkhris thermin.

2.We established that in the case of modelling of high alpha state-high oxy potential elevated

situation in rabbits by dinitrophenol (dinitrophenolmediated “proton loss process ”)are increased

the quantity of LPHD in a second compartment , decrease of LPLD,cholesterols,triglycerids in

the medium “between cell membrane structures-serum,representing a second

compartment”,paralleled with a high alpha state-high oxy potentials in membrane-redoxy

potentials three state line systems, ,exhibiting a sun like hot,hot oil external characteristics

,coded by abstract mkhris thermin.



3.We observed that in the case of modelling of high solid betta state mediated high reductionpotential elevated situation in rabbits by rotenone (by blocking cytochrome oxidase in thecomplex 4 with elevation of reduced form of NADH ) are provocated the decrease of bodytemperature , increase of reduced form of NADPH contents, decrease of oxidized form of NADP contents ,deline of oxidizing activity of hemaglobine molecules , increase of reduction:oxidation ratio in a first and fourth compartments ,elevation of reduction activity of membrane-

redoxy potentials three state line systems,increase of membrane resistance of membranestructures of 1 compartment ,decline of quantity of LPHD in second compartment, decrease of glucose utilization speed in second compartment with following increase of glucoseconcentration in a second compartment, decrease of oxidized- deprotonized products in a 5membrane structures,belonging to fourth compartment ,exhibiting a moon like cold, cool oilexternal characteristics ,coded by abstract badgan thermin.

4. We observed that in the case of modelling of high solid betta state mediated high reduction

potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in the

complex 4 with elevation of reduced form of NADH ) are decreased the quantity of LPHD in a

second compartment , increase of LPLD,cholesterols,triglycerids in the medium “between cell

membrane structures-serum,representing a second compartment”,paralleled with a high solidbetta state-high reduction potentials in membrane-redoxy potentials three state line systems,

,exhibiting a moon like cold, cool oil external characteristics ,coded by abstract badgan

thermin.

Definition of Mkhris abstract thermin.

Abstract Mkhris thermin showed :

a. Relatively high level of fluid alpha states,consisting of unsaturated fatty acids with high levels

of oxy potentials and with high levels of proton,electrons conductance and high levels heatenergy release, middle degree of energy accumulation and middle degree of high energy

phosphate-ATP, high energy electrons NADPH, with middle ratio of donators :accceptors are

associated with abstract theory of Mkhris,which is distinguished by hot,hot oil,acute external

characteristics.

b.The change of fourth compartment parameters ,named as 5 membrane structures-5 function

systems,ensuring normal genetic-cell division ,information-response ,biosynthetical ,

bioenergetical ,biotransformation functions by using of high energy phosphate-ATP, high energy

electrons NADPH and heat energy,generated in membrane-redoxy potentials three state line

systems in the form of unregulated intensification of information-response functions are coded

by abstract Mkhris code.

c.The Change of fourth compartment parameters ,named as 5 membrane structures-5

function systems,ensuring normal genetic-cell division ,information-response ,biosynthetical ,

bioenergetical ,biotransformation functions by using of high energy phosphate-ATP, high

energy electrons NADPH and heat energy,generated in membrane-redoxy potentials three state



line systems in the form of slowing of information-response functions are coded by abstract

Mkhris code in the Tibetian Mongolian Traditional medicine.

d. The change of Third compartment parameters in the form of increase of unsaturated class

of visceral and external under skin fatty acids, also decrease of donators, increase of acceptors

are coded by abstract Mkhris code in the Tibetian Mongolian Traditional medicine.

Definition of Badgan abstract thermin. Abstract Badgan thermin showed :

a. Relatively high level of solid betta state,consisting of mainly saturated fatty acids,

conditioning a high levels of red potentials and with slow levels of proton,electrons

conductance and low levels of heat energy release, high degree of energy accumulation and

high degree of high energy phosphate-ATP, high energy electrons NADPH, with increased ratio

of donators :accceptors are associated with abstract theory of Badgan ,which is distinguished by

cool ,cold oil, stupid external characteristics.

b.The change of fourth compartment parameters consisting of serum and extracellular

system,where donators,acceptors and some metabolites formed in the result of functioning of 5

membrane structures-5 function systems,ensuring normal genetic-cell division ,information-

response ,biosynthetical , bioenergetical ,biotransformation functions in the form of increase of

contents of donators, decrease of acceptors and increase of AO- decrease of SPOL system are

coded by abstract Badgan code in the Tibetian Mongolian Traditional medicine.

c.The change of fourth compartment parameters consisting of serum and extracellular



response ,biosynthetical , bioenergetical ,biotransformation functions in the form of decrease of

contents of donators, increase of acceptors and decrease of AO- increase of SPOL system arecoded by abstract Badgan code in the Tibetian Mongolian Traditional medicine.

d.The change of Third compartment parameters in the form of increase of saturated class of

visceral and external under skin fatty acids, also increase of donators, decrease of acceptors

are coded by abstract Badgan code in the Tibetian Mongolian Traditional medicine.

Definition of Rlung abstract thermin.

Abstract Rlung thermin showed :

a. Relatively high level of gamma state,consisting of decreased contents of saturated-unsaturated fatty acids, conditioning a decreased levels of redoxy potentials and with slow

levels of proton,electrons conductance and low levels of heat energy release and energy

accumulation and low degree of high energy phosphate-ATP, high energy electrons NADPH,

with decreased contents of donators :accceptors,increased significance of proton,electrons

prior to generation of proton gradients, prevailed slip mecchanism are associated with abstract

theory of rlung ,which is distinguished by light,mobile , nonoil, cool external characteristics.



b.The change of fourth compartment parameters, consisting of serum and extracellular



response ,biosynthetical, bioenergetical,biotransformation functions in the form of decrease of

contents of donators,acceptors and some metabolites formed in the result of functioning of 5 of

membrane structures-5 function systems,including synthetic, bioenergy,biotransformationfunctions are coded by abstract Rlung code in the Tibetian Mongolian Traditional medicine.

c.The change of fourth compartment parameters ,named as 5 membrane structures-5

function systems,ensuring normal genetic-cell division ,information-response ,biosynthetical ,

bioenergetical ,biotransformation functions by using of high energy phosphate-ATP, high energy

electrons NADPH and heat energy,generated in membrane-redoxy potentials three state line

systems in the form of decrease of synthetic, bioenergy,biotransformation functions and of

unregulated disturbance of information-response functions are coded by abstract Rlung code

in the Tibetian Mongolian Traditional medicine.

d.The change of third compartment parameters in the form of decrease of visceral and

external under skin fatty acids, also donators,acceptors are coded by abstract Rlung code in

the Tibetian Mongolian Traditional medicine.

References:

1. Ambaga M, Sarantsetseg B, “Rlung, mkhris, badgan- membrane structures”, UB,1995, 108 p.

2. Ambaga M, Sarantsetseg B, “Additional lectures on Traditional medicine” 2008, 109 p.

3. Ambaga M, Sarantsetseg B, “100 questions and answers of Traditional Medical Studies”, UB,

2005, 80 p..

4. Ambaga M, Sarantsetseg B, “Experimental research results,obtaining of 50 principal

decisions in framework of new conception of three membrane-redoxy potentials line systems

as main code of new medical system-NCM” UB, 2009, 80 p..

5. Ambaga M, Sarantsetseg B, “Explanation of some theoretical questions of Traditional

medicine at the level of cells, membranes” UB,2002 , 250 p.

6. Ambaga M, Sarantsetseg B, “Comparative study of basic theories of Oriental medicine/

similarities and differences” UB,1997, 106 p..

7. Ambaga M, Sarantsetseg B, Bold Sh , Philosophy, cognition and scientific bases of Oriental

Medicine” UB,1996, 109 p..

8. Ambaga M, Sarantsetseg B, “Code conversion between theoretical aspects of Traditional

and Modern medicine” UB,2005, 180 p..

9. Ambaga M, Sarantsetseg B, “Theoretical and methodological bases of united diagnosing and

therapy chain in Traditional and Modern Medicine”,UB,2005, 200 p.



10. Ambaga M, Sarantsetseg B, “The theoretical aspects of producing and activation of all

medicaments through two main conveyer system,including industry and living cells,”UB, 2008,

p.108.

11. Ambaga M, Sarantsetseg B, “Integration of Traditional Medicine and Pharmacy in the frame

of conception-two steps of producing and activation of medicinal products” UB, 2008, p.180.

12.Bhagwan Dash,Concept of agni in Ayurveda,1971,212 p.

13.Dwarakanath C,Digestion and metabolism in Auyrveda,2003,360 p.

14.Hyun-Ju Kim, Seung Yeon Hwang, Ju-Ho Kim et all, Association between genetic

Polymorphism of multidrug resistance 1 gene and Sa sang constitutions, Evidence-Based

Complementary and Alternative Medicine,2009,vol.6,S1,p.73-80.15. Micheal Heinrich,Joanne

Barnes,Simon Gibbons,Fundamentals of Pharmacognosy and phytotherapy,churchill

livingstone.16. Voet D, Biochemistry 1990,1221 p.

Documents

To Question of Creating of Experimental Models, Coded by Abstract Rlung, Mkhris, Badgan Thermins in Traditional Medicine