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Zoulin - Biospectrum d.o.o. Edini in ekskluzivni zastopnik in prodajalec, marketing ter promocija za Slovenijo, Hrvaško, Srbijo, Bosno in Hercegovino, Kosovo, Črno Goro, Makedonijo in Albanijo. Building# 1, Block #15, ABP. #188, 4th Southern Ring West Rd. Fengtai District, Beijing, China 100070 Tel: +86 10 63703006 Fax: +86 10 68573156 www.zhoulin.com.cn Zastopnik: Zhang Jie - International Department.

Treatment Clinical Reports Zhoulin Bio Spectrum

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Page 1: Treatment Clinical Reports Zhoulin Bio Spectrum

Zoulin - Biospectrum d.o.o.

Edini in ekskluzivni zastopnik in prodajalec, marketing ter

promocija za Slovenijo, Hrvaško, Srbijo, Bosno in

Hercegovino, Kosovo, Črno Goro, Makedonijo in Albanijo.

Building# 1, Block #15, ABP. #188, 4th Southern Ring West Rd.

Fengtai District, Beijing, China 100070

Tel: +86 10 63703006 Fax: +86 10 68573156

www.zhoulin.com.cn

Zastopnik: Zhang Jie - International Department.

Page 2: Treatment Clinical Reports Zhoulin Bio Spectrum

Opatje selo 7A, 5291 Miren, SLOVENIA – EU

www.biospectrum.biz [email protected]

Mobile: 031 566 977

Promocija, Marketing, Trženje

Andrej Keber BE

Mobile: +386 (0)41 719 321

Mail: [email protected] [email protected]

Zoulin - Biospectrum d.o.o.

Page 3: Treatment Clinical Reports Zhoulin Bio Spectrum

Training on Treatment Mechanism Of

Zhoulin Bio-spectrum Treatment Device

and Clinical Reports

Page 4: Treatment Clinical Reports Zhoulin Bio Spectrum

Content

Introduction of Zhoulin Bio-spectrum technology Co. Ltd

1. About Zhoulin

2. About Bio-spectrum

3. Treatment mechanism

4. Advantages over similar products

Clinical reports

I. clinical studies and mechanism of respiratory disease

II. Peripheral nerve disease-related clinical trials and mechanism

III. clinical trials and mechanism of Digestive disease

IV. Ischemic disease, and red blood cell rheology

V. Efficacy on wound healing and mild burns, bedsores by Zhoulin BFS Device

VI. Zhoulin BFS can lower blood glucose levels

Page 5: Treatment Clinical Reports Zhoulin Bio Spectrum

Zhoulin Bio-spectrum Treatment

Device can simulate the

electromagnetic spectrum of human

body. Through resonant absorption

(the spectrum is matched and absorbed

by the body), it can produce thermal

effect and non-thermal effect within

the body for a beneficial regulation of

human spectrum which results in an

auxiliary treatment on disease of

several human body systems. Its main

functions include: improve blood

circulation and blood rheology;

enhance immune function; regulate

nervous system function; promote

metabolism.

Page 6: Treatment Clinical Reports Zhoulin Bio Spectrum

About Mr.Zhoulin:

• Inventor of the Bio-spectrum Treatment Device

• Founder of the Medical Bio-spectrum

• NPC representatives of the 7th and 8th National

People's Congress

• Vice President of China Association of

Inventions

• Chief Scientist and Host of Zhoulin laboratory

in United States

•1985 WIPO (World Intellectual Property Organization) Invention and Innovation Award

•1991 Patented in China

•1996 Patented in US and EU

•2000 Passed CE certification

•2008 Official sponsorship of the 2008 Paralympic Games

•2009 China's Top 60 Brands

•2010 Asia Top 500 brands

Page 7: Treatment Clinical Reports Zhoulin Bio Spectrum

Zhoulin Bio-spectrum has

become the leading brand in

healthcare and rehabilitation

industry in the past 30 years.

Company Headquarter

Page 8: Treatment Clinical Reports Zhoulin Bio Spectrum

Gold Medal of WIPO (World Intellectual Property Organization)

Invention and Innovation Award

Page 9: Treatment Clinical Reports Zhoulin Bio Spectrum

Independent Intellectual Property

Ten Thousand of Clinical Reports

Page 10: Treatment Clinical Reports Zhoulin Bio Spectrum

About Bio-spectrum

All matters emit certain kind of electromagnetic spectrum at the

temperature above absolute zero. Our researches have indictated

that the electromagnetic spectrum emitted by living creature

ranges from infrared wave to microwave,which is called"Bio-

spectrum ." Human body is an irradiation source of low energy.

Absolute zero: The lowest temperature theoretically attainable, there is no

object at this temperature who has internal energy. -273.15 ℃ set for the

thermodynamic temperature scale (Kelvin) of zero, is called absolute zero.

Bio-spectrum(Biofrenquency Spectrum) is an integrated title for the

frequency of biological information of living creatures and the spectrum.

Page 11: Treatment Clinical Reports Zhoulin Bio Spectrum

Infrared images of

buildings

Infrared images of human

body

Passive millimeter wave images of human

body

Infrared imaging and millimeter-

wave imaging

Page 12: Treatment Clinical Reports Zhoulin Bio Spectrum

Zhoulin Bio-spectrum Treatment Device owns the wide frequency band. The main

energy zone lies in the infrared section and spreads out to the millimeter and microwave

section(weak energy zone), which covers the similar frequency area of human body.

The difference of peak position of waves is similar to the characteristics of the

absorption spectral band of human body.

For weak energy zone, including infrared and micro-millimeter wave, though not so

strong direct effect on human body, however, laboratory animal studies and clinical

practice show some effect is from the distribution of spectral characteristics rather

than intensity of radiation. The non-thermal effect of Zhoulin Bio-spectrum

Treatment Device is closely related to this part of energy zone.

Zhoulin Bio-spectrum Treatment Device

Page 13: Treatment Clinical Reports Zhoulin Bio Spectrum

What is matching absorption (Resonant Absorption)?

When the frequency and amplitude of the external

radiation is close to the natural frequency of the

human body, the body achieves a maximum

absorption of the radiation. This is called the

resonant absorption.

Page 14: Treatment Clinical Reports Zhoulin Bio Spectrum

What is the endo-thermal effect?

Cells are basic unit of the human body . The composition of

molecules and charged particles absorbs the radiation and causes the

temperature rise of the corresponding tissues, which is so called

"endo-thermal effect." While also the radiation changes the membrane

potential (细胞膜电位) to promote the exchange of intracellular

substances, as well as biochemical effects of synthesis and

decomposition, etc.

Page 15: Treatment Clinical Reports Zhoulin Bio Spectrum

What is Non-thermal effect?

Non-thermal effect refers to a series biological effects of living creatures which

produce no significant heat and no obvious increase in body temperature after

receiving electromagnetic radiation. The biological effects which are difficult to

be explained by thermal effects are known as non-thermal effects.

Non-thermal effects are produced from millimeter-wave and weak microwave in

weak energy zone. A large number of biological experiments on millimeter-wave

have verified the existence of non-thermal effects. Most of the energy needed to

generate shock is provided by the biological system itself (which means the

body's own energy), and millimeter-wave only plays the role of the trigger.

(Similar to the role of the first domino)

Page 16: Treatment Clinical Reports Zhoulin Bio Spectrum
Page 17: Treatment Clinical Reports Zhoulin Bio Spectrum

Difference Between Zhoulin Bio-spectrum Treatment Device and other

similar products

Due to different treatment mechanism, the application range of Zhoulin Bio-spectrum Treatment Device approved by SFDA is wider than that of TDP.

Zhoulin Bio-spectrum Treatment Device has ten thousand of clinical

reports over the 30 years to prove its good efficacy, convenience and safe in

application.

Internationally Patented, leading brand, good reputation

Zhoulin Bio-spectrum is widebrand spectrum with thermal effect and non-

thermal effect. Compared to TDP, it has wider range of applications and

better efficacy.

Page 18: Treatment Clinical Reports Zhoulin Bio Spectrum

Application of Zhoulin Bio-spectrum Treatment Device:

soft tissue sprains, muscular pain, arthritis, cervical spondylosis, wound

infection and healing,chronic prostatitis, bronchitis, bronchial asthma,

chronic gastritis, duodenal ulcer, chronic hepatitis, colitis, constipation,

diarrhea, shingles, eczema, neurodermatitis mild burns, bedsores, frostbite,

chronic pelvic inflammation, dysmenorrhea, paediatric pneumonia,

mumps.

Page 19: Treatment Clinical Reports Zhoulin Bio Spectrum

Application of TDP:Closed soft tissue injury, acute and chronic rheumatoid

arthritis, periarthritis humeroscapularis, paediatric diarrhea.

Page 20: Treatment Clinical Reports Zhoulin Bio Spectrum

Basic Researches

Clinical Data

Application

Animal Studies

The scientific research methods of Zhoulin Bio-spectrum

Page 21: Treatment Clinical Reports Zhoulin Bio Spectrum

I. clinical studies and mechanism of respiratory disease

Page 22: Treatment Clinical Reports Zhoulin Bio Spectrum

Studies on WS Series Treatment Device to

regulate the immune function of rats

Lin JiaYou Gao Yang Li Li

Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences

Article 1

Page 23: Treatment Clinical Reports Zhoulin Bio Spectrum

Experimental Principle

Irradiate normal and tumor-bearing mice with WS Series Bio-

spectrum Treatment Device

Observe its inluence on the activity of the spleen NK cells and the

impact of the mouse antibody

Observe change of PHA proliferative responses of the mouse immune

cells

NK cell: Natural killer cells. It is an important immune cell of the body which is related to

prevent tumor and virus infection as well as being involved in autoimmune disease.

PHA proliferative responses: a kind of approach to observe the immune responses of

stimating lymphocytes with PHA (phytohaemagglutinin)

Page 24: Treatment Clinical Reports Zhoulin Bio Spectrum

Different inluence on the activity of the spleen NK cells by Bio-spectrum Treatment Device

n irradiated Group n Control Group P-Value

Tumor-

bearing

mice

10 8.54±2.98 10 4.09±0.95 <0.01

normal

mice

10 17.06±8.09 10 6.3±2.94 <0.01

Different impact of the mouse antibody by Bio-spectrum Treatment Device

n irradiated Group n Control Group P-Value

Tumor-

bearing

mice

16 0.58±0.17 11 0.71±0.10 <0.05

normal mice 17 0.66±0.10 14 0.79±0.06 <0.01

Page 25: Treatment Clinical Reports Zhoulin Bio Spectrum

Different change of PHA proliferative responses of the mouse spleen cells by Bio-spectrum

Treatment Device

n irradiated Group n Control Group P-Value

Tumor-

bearing mice

10 15520±5757 10 2931±993 <0.01

normal mice 10 96263±4565 10 35369±12269 <0.01

Page 26: Treatment Clinical Reports Zhoulin Bio Spectrum

Conclusion The study shows:

To irradiate the normal and tumor-bearing mice with Bio-spectrum Treatment

Device can increase the activity of NK cells and enhance the PHA proliferative

responses of T cells.

To irradiate the normal and tumor-bearing mice with Bio-spectrum Treatment

Device can produce antibodies that play a suppressive regulatory role, which

means it can help with treatment of antibody-mediated autoimmune disease. For

example: lupus erythematosus (红斑狼疮), hepatitis,etc.

Conclusion: Bio-spectrum Treatment Device can promote immunity of the

body.

Page 27: Treatment Clinical Reports Zhoulin Bio Spectrum

Efficacy Observation and Nursing Care on Acute

Bronchitis of the Elderly by Zhoulin Bio-Spectrum

Treatment Device

Article 2

Mei Zhen

Geriatrics Section II, PLA Fuzhou General Hospital

Page 28: Treatment Clinical Reports Zhoulin Bio Spectrum

General Information and Methods

92 patients with Acute Bronchitis were divided into two groups

randomly: treatment group and control group.

treatment group: spectrum device (irradiate patient’s chest) +

conventional drug therapy

control group: conventional drug therapy

Effective rate: Fully recovered, Marked effect, Improved, No

effect.

Page 29: Treatment Clinical Reports Zhoulin Bio Spectrum

Efficacy Observation and Nursing Care on Acute Bronchitis of the Elderly

by Zhoulin Bio-Spectrum Treatment Device

Conclusion:

Most elderly patients with bronchitis have low immunity and poor blood circulation

function. To irradiate their chest with the spectrum device can promote blood

circulation, improve immune status, speed up the absorption of local inflammation, and

improve respiratory function. With conventional anti-inflammatory drug therapy and

Zhoulin Bio-spectrum Treatment Device can quickly improve the clinical symptoms and

shorten the treatment time.

Page 30: Treatment Clinical Reports Zhoulin Bio Spectrum

WS-spectrum Device Effect on induction level of

TNF-α and plasma IL-8 levels for patients with

asthma

Liang Zhihui, Feng Wei, Wang Liren Section of Immunology, Tongji Medical University Zhong RuiSheng Liu Guanghui Section of Allergy , Tongji Hospital Zhai liXia Pharmacy Department, Wuhan Railway Central Hospital

Article 3

Page 31: Treatment Clinical Reports Zhoulin Bio Spectrum

Knowledge on asthma:

Asthma is a common lung disease for all age groups. Its incidence is rising. Pathology and

immunology prove that airway inflammation is related to the cause of asthma, whereas the

body's immune system is the medium of inflammatory response, in this process, some

inflammatory cytokines play an important role in the inflammatory response. Such as TNF-α,

IL-8 and so on.

Experimental design

To study the treatment mechanism of Zhoulin Bio-spectrum Device on asthma, we test the

induction levels of TNF-α and IL-8 levels of the patients with asthma before and after the

treatment and that level of normal people. And also we study the effect of the 2 levels on treating

asthma when using the spectrum device.

TNF-α: It is tumor necrosis factor, which can promote T cells to produce various inflammatory cytokines, thus

contributing to inflammatory reactions.

IL-8: Interleukin-8. In the inflammatory response, the IL-8 induces neutrophils and T cells to the lesion site.

Page 32: Treatment Clinical Reports Zhoulin Bio Spectrum

Group No. of Patients Induction level of TNF-α

Normal people group(A)

Before treatment(B)

After treatment(C)

20

13

13

664.56±316.18

2332.80±944.5

1490.61±689.77

Before treatment,induction level of TNF-α is obviously higher than that of normal people group.

(P<0.001),whereas, the level after treatment by spectrum device is much lower than that before treatment.

(P<0.05)

Group No. of Patients IL-8 level

Normal people group(A)

Before treatment(B)

After treatment(C)

21

14

14

169.62±7.27

181.91±23.63

182.16±35.77

Comparison of plasma IL-8 levels (Table 2)

WS-spectrum Device Effect on induction level of TNF-α for patients with asthma

(Table 1)

Before treatment, the plasma IL-8 level is higher than that of normal people group.

(P<0.05),whereas ther is no significant difference between the levels before and after treatment.

(P>0.05)

Page 33: Treatment Clinical Reports Zhoulin Bio Spectrum

Experimental Conclusion

TNF-α is an inflammatory mediator of broad biological activity produced by activated monocyte-macrophage cells, which can directly induce bronchoconstriction to cause asthma. IL-8 from being discovered, is considered an inflammatory mediator, and so far, extensive documents demonstrate their role of inflammatory mediators. The results show that Treatment mechanism of ZhouLin Bio-spectrum Device on effectively control of asthma is to reduce the high response of macrophages to lower the induction level of TNF-α to reduce the role of inflammatory mediators, which alleviate the asthma in the end. Tips: Spectrum Device is an effective auxiliary therapy for asthma.

Page 34: Treatment Clinical Reports Zhoulin Bio Spectrum

Efficacy Observation of Zhoulin Bio-spectrum Treatment

Device on Bronchial Asthma

Li Xueqian

Pulmonary Section, JiangHan 1st branch institute, The Union Hospital Affiliated to

Tongji Medical University;

Wu Qingping

Management Department, Wuhan Workers' Medical College

Article 4

Page 35: Treatment Clinical Reports Zhoulin Bio Spectrum

Male Female Total

Inhaled asthma

Infection-induced asthma

Chronic asthmatic bronchitis

Total

44

37

26

107

10

11

8

29

54

48

34

136

Effective Not

Effective

Total Effective Rate

(%)

Inhaled asthma

Infection-induced asthma

Chronic asthmatic bronchitis

Total

46

36

20

102

8

12

14

34

54

48

34

136

85.2

75.0

58.8

75.0

Efficacy of three groups of patients

Gender composition of three groups of patients

Spectrum Device is an effective auxiliary therapy for different kinds of asthma.

Page 36: Treatment Clinical Reports Zhoulin Bio Spectrum

Auxilliary treatment of Zhoulin Bio-spectrum Treatment Device

on respiratory disease

Summary

Page 37: Treatment Clinical Reports Zhoulin Bio Spectrum

Major respiratory disease:

acute and chronical tracheobronchitis,bronchial asthma, pneumoniae,etc

Cause:

pollution, infection, allergy, low

immunity, etc.

Clinical Therapy:

Antibiotic treatment,

Oxygen and mist inhalation

Symptomatic treatment

Page 38: Treatment Clinical Reports Zhoulin Bio Spectrum

Enhance Immune System

Adjust local micro-circulation to strengthen phagocytes, accelerate cilia

activity, promote expectoration

Improve the metabolism of the lesion

For asthma, besides all the functions above, the spectrum device can

reduce the induction of TNF-α of inflammatory mediator to alleviate the

asthma.

Auxilliary treatment mechanism of Zhoulin Bio-spectrum Treatment Device on

respiratory disease

Page 39: Treatment Clinical Reports Zhoulin Bio Spectrum

User's Guide on pneumoniae and bronchial asthma

Location of irradiation:

the affected part and acupuncture

point: 6,9,11

Number of times and time:

2 times/day

30 min/time

Acupoint of chest and

abdomen

Notice:

Better be used with conventional

drug therapy

acupoint for

treating

pneumoniae and

bronchial asthma

Page 40: Treatment Clinical Reports Zhoulin Bio Spectrum

Acupoints of back, waist and hip

acupoint for treating

pneumoniae and

bronchial asthma

Page 41: Treatment Clinical Reports Zhoulin Bio Spectrum

User's Guide on bronchial asthma

Location of irradiation:

acupuncture point: 4, 6

Number of times and time:

2-3 times/day

40 min/time

acupoint of

bronchial asthma

Page 42: Treatment Clinical Reports Zhoulin Bio Spectrum

II. Peripheral nerve disease-related clinical

trials and mechanism

Page 43: Treatment Clinical Reports Zhoulin Bio Spectrum

Treatment mechanism of WS bio-spectrum Device on peripheral

nerve disease

Xiao Wenhua Xie Yi

Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences

Article 1

Page 44: Treatment Clinical Reports Zhoulin Bio Spectrum

Irradiate the sciatic nerve (坐骨神经)

damage model of SD rats with

spectrum device to observe the

conductibility of the damaged nerve

and recovery of action potentials (动作电位) as well as response to

medication. (irradiate the control group

with infrared light)

Experimental Principles

Page 45: Treatment Clinical Reports Zhoulin Bio Spectrum

Experimental Conclusion

Spectrum Irradiation Group: It can restore part of the action potential of the damaged nerve, so that the impulse conduction of the damaged nerve changes from jumpy conduction to continuous conduction. And it helps to restore the normal structure of the molecular layer membrane. Infrared Irradiation Group: To irradiate the damaged nerve in animals with the infrared light of the same power. the damaged nerve zone shows no improvement on their reaction to TEA and anisodamine as well as transmission of nerve impulses as same as the group with no treatment. Important notes: Thermal effect is not the main factor to restore nerve function. The non-thermal effect of Zhoulin Bio-spectrum Treatment device is the main factor.

Page 46: Treatment Clinical Reports Zhoulin Bio Spectrum

Study on 64 patients withBell's Palsy by Zhoulin Bio-spectrum

Treatment Device

Chen Aihua

The First People’s Hospital of Jining City

Article 2

Page 47: Treatment Clinical Reports Zhoulin Bio Spectrum

Treatment Group:medicine+spectrum device Control Group I:medicine+TDP Control Group II: medicine

Conclusion:

The facial nerve of the patients with Bell's palsy is caused vascular spasm and ischemia due to

physical factors or viral infection to result in neural stem edema and then demyelination.

Conventional treatment is methyl vitamin B12 therapy or hormone therapy but with no obvious

changes on early pathology. Zhoulin Bio-spectrum Treatment Device can improve local

circulation and rapidly reduce swelling, and restore the action potential of neural stem and repair

medullary sheath as well.

Zhoulin Bio-spectrum Treatment Device is an effective auxiliary therapy for facial paralysis.

Page 48: Treatment Clinical Reports Zhoulin Bio Spectrum

Treatment mechanism of WS Bio-spectrum Device on peripheral

nerve disease

Summary

Page 49: Treatment Clinical Reports Zhoulin Bio Spectrum

Cervical spondylosis, lumbar disease,

bone hyperplasia, facial paralysis, etc are

similar in pathogenesis which is caused by

neural stem edema and demyelination as well

as a series of symptoms caused by the trapped

nerve stem due to bone spur.

Causes: degeneration, strain,etc.

Treatment: surgery, medication, massage,

etc.

Knowledge on hyperosteogeny

Page 50: Treatment Clinical Reports Zhoulin Bio Spectrum

Treatment mechanism of WS Bio-spectrum Device on peripheral nerve disease

1. improve local blood circulation, remove the algogenic substance

2. Spectrum Device can restore part of the action potentials of damaged nerve

and improve the impulsive conductibility

3. Alleviate local edema

Algogenic substance: Potassium, hydrogen, calcium ions

play important role in causing pain in tissue damage or

inflammation, which are the main algogenic substances of

the disc herniation, rheumatoid arthritis, cancer,etc. And

other substances include serotonin, prostaglandin G,

bradykinin, etc.

Page 51: Treatment Clinical Reports Zhoulin Bio Spectrum

Auxilliary Treatment on Cervical spondylosis

Location of irradiation:

acupuncture point: 9

Number of times and time:

2 times/day

30 min/time

acupoint of

Cervical

spondylosis

Page 52: Treatment Clinical Reports Zhoulin Bio Spectrum

Auxilliary Treatment on lumbar disease, strain

Location of irradiation:

the affected part and acupuncture point:

11,29

Number of times and time:

2 times/day

30 min/time

acupoint of lumbar

disease, strain

Page 53: Treatment Clinical Reports Zhoulin Bio Spectrum

acupoint of lumbar disease,

strain

Page 54: Treatment Clinical Reports Zhoulin Bio Spectrum

Auxilliary Treatment on facial paralysis

Location of irradiation:

From behind the ear to the facial area

with facial nerve distribution

Number of times and time:

2 times/day

30 min/time

acupoint of facial

paralysis

Page 55: Treatment Clinical Reports Zhoulin Bio Spectrum

III. clinical trials and mechanism of Digestive

disease

Page 56: Treatment Clinical Reports Zhoulin Bio Spectrum

Study on treatment mechanism of ulcers by Zhoulin

Bio-spectrum Device

Dong XiuYun Deng Wenlin Liu Xiumin

Department of Gastroenterology, Third Hospital of Beijing

Medical University

Article 1

Page 57: Treatment Clinical Reports Zhoulin Bio Spectrum

Peptic ulcers

Peptic ulcers: main causes: gastric acid, HP.

The healing of ulcers is related to many factors, among which

gastric mucosal blood flow is an important factor. Others factors

include oxygen free radicals, pepsin, glucoprotein, gastric acid and

so on.

Biological spectrum device has been found to promote healing of

gastric ulcers in rats. This study helps to prove the previous

foundings by these factors above.

Page 58: Treatment Clinical Reports Zhoulin Bio Spectrum

Group n Area of ulcers(mm) P

A Control Group

B BFS Group

C Infrared Group

D Lansoprazole Drug Group

E Lansoprazole Drug plus BFS

Group

8

8

8

8

7

9.5±2.2

5.9±1.4

6.1±1.8

5.2±1.5

4.2±1.6

P<0.05

P<0.05

P<0.05

P<0.01

Study on healing of acetic acid type gastric ulcers of the rats by

Zhoulin Bio-spectrum Device(Table 1)

Zhoulin Bio-spectrum Device can promote the healing of acetic acid type

gastric ulcers of the rats. Group E ( treated with Lansoprazole Drug and the

spectrum device together) is found obvious smaller area of ulcers. The efficacy

of Infrared Group is not as good as that of BFS group.

Page 59: Treatment Clinical Reports Zhoulin Bio Spectrum

Group n GMBF(ml/min/100g) P

A Control Group

B BFS Group

C Infrared Group

D Lansoprazole Drug Group

E Lansoprazole Drug plus BFS

Group

8

8

8

8

7

41.71±11.6

59.33±12.4

54.10±17.2

35.60±21.2

46.83±19.4

P<0.05

P<0.05

P>0.05

P>0.05

Effect on gastric mucosal blood flow of the rats by Zhoulin Bio-

spectrum Device(Table 2)

The gastric mucosal blood flow (GMBF) of BFS Group and Infrared Group is

obviously increased. Group D (Lansoprazole Drug Group) is seen decreased while

Group E (combination treatment of spectrum device and BFS) tends to increase.

Page 60: Treatment Clinical Reports Zhoulin Bio Spectrum

Experimental Conclusion

The increase of gastric mucosal blood flow is an important factor of the healing of

gastic ulcers. The reasons why Zhoulin Bio-spectrum Device can promote the

healing of gastric ulcers include that it can increase gastric mucosal blood flow and

mucosal secretion of glucoprotein as well as reduce oxygen free radicals. In addition,

Zhoulin Bio-spectrum Device can effectively reduce the recurrent of the ulcers.

Though Infrared Group has shown some improvement on the the ulcers, but the

efficacy is much smaller than that of the spectrum device group.

Zhoulin Bio-spectrum Device can promote the healing of gastric ulcers.

Page 61: Treatment Clinical Reports Zhoulin Bio Spectrum

Therapeutical Effects of BFS on Peptic Ulcer - Report of 48

Cases

Yang Biying, Long Xiaoguang,

Department of Physiotherapy, Hepingli Hospital, Beijing

Wang Facheng, Zhang Min,

Department of Internal Medicine, Hepingli Hospital, Beijing

Article 2

Page 62: Treatment Clinical Reports Zhoulin Bio Spectrum

Data and Methods

Selection of cases: 48 cases with peptic ulcer in active

stage, diagnosed by endoscope. The patients were divided

into two groups: treatment group and control group.

Treatment Group:Tagamet+Cimetidine+Zhoulin BFS

Device Control Group:Tagamet+Cimetidine

Observation: pain relief time, time needed for stool

occult blood to turn negative, time for ulcer healing

Page 63: Treatment Clinical Reports Zhoulin Bio Spectrum

Treatment

Group

Control

Group

t P

time needed for stool

occult blood to turn

negative

9.5±2.41 7.6±1.38 1.84 >0.05

Treatment

Group

Control

Group

t P

pain relief time 3.94±1.9 7.38±5.74 2.429 <0.05

Time for ulcer healing Number of

treatment group

Number of

Control Group

P

Recovery within 2

weeks

Recovery within 4

weeks

8

20

6

17

>0.05

>0.05

Time needed for stool occult blood to turn negative

pain relief time

Time for ulcer healing

Page 64: Treatment Clinical Reports Zhoulin Bio Spectrum

Experimental Conclusion

We observe the recent efficacy on peptic ulcers by Zhoulin

Bio-spectrum Treatment Device, which shows a significant

efficacy on pain relief. For the healing of ulcers, especially

in treatment of the bulbar ulcer with bulbar deformation, the

efficacy of the treatment group is much better than that of

the Control Group.

Zhoulin Bio-spectrum Treatment Device is an effective

auxiliary treatment of peptic ulcers.

Page 65: Treatment Clinical Reports Zhoulin Bio Spectrum

Summary

Study of the auxiliary treatment on digestive disease by

Zhoulin Bio-spectrum Treatment Device

Page 66: Treatment Clinical Reports Zhoulin Bio Spectrum

Digestive disease

The digestive disease can be treated

by Zhoulin include: acute and chronic

gastritis, gastric ulcers, duodenal

ulcers, enteritis, diarrhea, etc.

Treatment:

mucosal protective agent

Antacid

Antibiotics

Surgery

Symptomatic treatment

Page 67: Treatment Clinical Reports Zhoulin Bio Spectrum

Functions of Zhoulin Bio-spectrum Treatment

Device: * increase gastric mucosal blood flow

* increase mucosal secretion of glucoprotein

* reduce oxygen free radicals.

* effectively reduce the recurrent of the ulcers and relieve

pain

* better efficacy can be achieved when using with antacid

medicine

Auxiliary treatment on digestive disease by Zhoulin Bio-

spectrum Treatment Device

Glucoprotein: Mucosal surface is covered with a layer of mucus. Mucus glucoprotein is

the main component to form the mucus viscosity and gel-forming ability.

Page 68: Treatment Clinical Reports Zhoulin Bio Spectrum

Location of irradiation:

the affected part and

acupuncture points: 7,10,24

Number of times and time:

1-2 times/day

40 min/time

acupoint of chronic

gastritis, duodenal

ulcers

Acupoints for treating chronic gastritis, duodenal ulcers

Page 69: Treatment Clinical Reports Zhoulin Bio Spectrum

Acupoints for treating chronic

gastritis, duodenal ulcers

Acupoints for treating chronic gastritis, duodenal ulcers

Page 70: Treatment Clinical Reports Zhoulin Bio Spectrum

IV. Ischemic disease, and red

blood cell rheology

Page 71: Treatment Clinical Reports Zhoulin Bio Spectrum

Effect of red blood cell rheology by Zhoulin Bio-

spectrum Treatment Device

Xu Jialing, Zhang Chuanli, Su Gang, Yu Guifen

Department of Biophysics, Beijing Medical University

Article 1

Page 72: Treatment Clinical Reports Zhoulin Bio Spectrum

Experiment Principles

Red blood cells cover the most

percentage of the blood and has

the greatest impact on blood

rheology. The deformation of red

blood cells is the necessary factor

to ensure the effective perfusion

of the microcirculation. Therefore,

we use the spectrum device to

irradiate blood in vitro and the

whole body of animals to test the

change of the red cell membrance

fluidity, electrophoretic mobility,

and the ability of deformation

before and after irradiation, by

which we can carry out further

studies.

Normal Red Blood

Cells

Page 73: Treatment Clinical Reports Zhoulin Bio Spectrum

Irradiation of Red Blood Cells in Vitro

38℃ 40-42℃

water bath

42℃

Irradiation of whole body

1st day 8th day

Control Group

N

Experiment Group

N

P-value

2.26±0.16 2.16±0.14

12 42

2.31±0.13 2.87±0.17

14 42

>0.05 <0.01

2.26±0.16

12

2.07±0.12

16

<0.05

2.01±0.17 2.07±0.10

18 22

2.28 ±0.13 2.44 ±0.14

20 24

<0.01

Impact of Zhoulin BFS on the change of electrophoretic mobility of

red blood cells

Experiment Shows after irradiation, the electrophoretic mobility is

higher than that before irradiation.

Page 74: Treatment Clinical Reports Zhoulin Bio Spectrum

Experimental Conclusion

Red blood cells have aggregation and variability. Surface charge of red blood cells is

related to red blood cell aggregation. Some of the disease caused by increased blood

viscosity such as hyperlipidemia, coronary heart disease, diabetes have lower

electrophoretic mobility, which indicates that red blood cells is easy to gather to cause

the occurrence of thrombosis due to decreased cell surface charge density. Zhoulin BFS

device can increase red blood cell electrophoretic mobility, which means that irradiation

with Zhoulin BFS device can reduce red blood cell aggregation to promote

microcirculation. In the meanwhile, irradiation can increase red blood cell deformability

which also contributes to the improvement of microcirculation.

Irradiation with Zhoulin BFS device can reduce red blood cell aggregation, ease

blood viscosity, and prevent thrombosis

Page 75: Treatment Clinical Reports Zhoulin Bio Spectrum

Effect on cerebral blood supply for patients with cervical

spondylosis treated by Zhoulin BFS device

Ji Yanbin, Wang Laiyuan, Li Xiaofeng, Gao Chong

Department of Immunology, Second Affiliated Hospital of Shanxi Medical Colleage

Shi Ruyuan

Department of Inspection, Second Affiliated Hospital of Shanxi Medical Colleage

Article 2

Page 76: Treatment Clinical Reports Zhoulin Bio Spectrum

patient selection and group:

30 patients with cervical spondylosis, average age is 45, and age difference is

less than 5 years.

Patients are divided into 2 groups randomly:

Control Group: Venoruton 200 mg, Beniol 1 pill , 3 times/day.

Treatment group: irradiation with Zhoulin BFS Device. Centered with

acupoint of Dazhui, switch on high power, 30 min./ time, 2 times /day.

Treatment time: 2 weeks.

Frans-Scan 3D-TCD by EME Germany detects the speed of the blood flow.

Page 77: Treatment Clinical Reports Zhoulin Bio Spectrum

(A)Before

treatment(cm/s)

After treatment(cm/s)

(B)treat with

drug

(c)treat with

BFS device

BA 31.23±3.41(30) 44.11±5.23(12) 49.62±8.35(18)

LA 25.46±7.22(30) 31.42±4.26 (12) 37.66±7.25(18)

RA 23.15±4.64(30) 29.35±4.77(12) 33.61±4.31(18)

Effect on cerebral blood supply for patients with cervical spondylosis treated by

Zhoulin BFS device

Page 78: Treatment Clinical Reports Zhoulin Bio Spectrum

Experiment Conclusion

With irradiation of the basilar artery(BA), left artery (LA) and right artery (RA) for the

patients with cervical spondylosis by Zhoulin BFS device, the speed of blood flow is

obviously increased(P<0.01). Compared with the Control Group, the BFS Group can

significantly improve the blood flow of basilar artery and right artery (P <0.05), particularly,

the improvement of the left artery.

Experiment suggests that Zhoulin BFS device is more effective on the improvement of

the cerebral blood supply for patients with cervical spondylosis than some of the

medication.

Page 79: Treatment Clinical Reports Zhoulin Bio Spectrum

Auxiliary treatment of ischemic disease by Zhoulin BFS device

Summary

Page 80: Treatment Clinical Reports Zhoulin Bio Spectrum

Ischemic disease:

Ischemic disease is a class of diseases

caused by insufficient blood supply due to

all causes of vascular stenosis, which

includes: cerebral ischemia, coronary

heart disease, a variety of vascular disease,

diabetes mellitus complicated by vascular

lesion so on.

Cause:

Primary disease such as diabetes,

hypertension, renal disease,

bad habits, high cholesterol, obesity, etc.

Treatment: vasodilators, calcium

antagonists

Page 81: Treatment Clinical Reports Zhoulin Bio Spectrum

Auxiliary treatment of ischemic disease by Zhoulin BFS device

Surface charge of red blood cells is related to red blood cell aggregation .

Some of the diseases caused by increased blood viscosity such as

hyperlipidemia, coronary heart disease, diabetes have lower

electrophoretic mobility, which indicates that red blood cells is easy to

gather to cause the occurrence of thrombosis due to decreased cell

surface charge density.

Zhoulin BFS device can

increase red blood cell electrophoretic mobility

reduce red blood cell aggregation to promote microcirculation

increase red blood cell deformability

Page 82: Treatment Clinical Reports Zhoulin Bio Spectrum

V. Efficacy on wound healing and mild burns,

bedsores by Zhoulin BFS Device

Page 83: Treatment Clinical Reports Zhoulin Bio Spectrum

Study on the wound healing of the rat skin by BFS

Device

Li Changtian Dong Linwang

Institute of Cardiovascular Research, Beijing Medical University

Article 1

Page 84: Treatment Clinical Reports Zhoulin Bio Spectrum

Experiment

3 groups of healthy rats :

Surgery Group (S) Wound Group (C) Wound

+ BFS Group BFS Group(E) (Wound includes

cut or burnt )

Observation: change of body weight, the activity of

growth factor of skin(GFS), peroxidase of the burnt

tissues, etc.

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3 days 6 days 9 days

Group C

Group E

Group S

56.76±6.83

62.88±8.31

40.66±6.15

55.60±10,34

69.21±7.72

52.09±7.19

60.96±8.94

3 days 6 days 9 days

Group C

Group E

Group S

46.39±6.58

40.59±6.99

38.65±6.47

63.91±8.27

78.36±9.59

55.61±10.81

68.39±7.34

Change of GFs activity of the burnt tissues on the 3rd, 6th, 9th

day

Group E : Group C:P<0.05

Change of GFs activity of the hurt tissues on the 3rd, 6th, 9th day

Group E : Group C:P<0.05

Page 86: Treatment Clinical Reports Zhoulin Bio Spectrum

Experimental Discussion

Zhoulin bio-spectrum is a electromagnetic wave of relatively broad spectral range.

Numerous clinical and experimental studies have found that the spectrum device

can improve local circulation and metabolic disorders. The followings are

confirmed by this study:

1. Zhoulin BFS Device can regain the body weight of the hurt rats

2. reduce the tissue damage, can inhibit the increase of vascular permeability at

early stage

3. promote recovery of wound tissue, can promote the activity of fibroblast

growth factor (GFs) and increase collagen synthesis

Irradiation with Zhoulin BFS device can accelerate wound healing.

Page 87: Treatment Clinical Reports Zhoulin Bio Spectrum

Efficacy study on 33 Cases of wound infections

treated by WS-111 Bio-spectrum Treatment

Device

Department of Physical Rehabilitation, Beijing Friendship Hospital

Article 2

Page 88: Treatment Clinical Reports Zhoulin Bio Spectrum

Efficacy study on 33 Cases of wound infections treated by WS-111

Bio-spectrum Treatment Device

Basic recovery rate of Zhoulin BFS is 84.8%, and basic recovery rate of TDP

is 37.5%.

Page 89: Treatment Clinical Reports Zhoulin Bio Spectrum

Zhoulin BFS device has the following effects on wound

healing

It can inhibit the increase of vascular permeability, reduce tissue edema at the

early stage;

It can promote recovery of wound tissue, can promote the activity of epidermal

growth factor( EGF) and fibroblast growth factor (GFs) at the early stage and

promote collagen synthesis;

It can promote blood circulation, speed up the accumulation of immune cells to

the wound to prevent infection;

The speed-up local circulation can take away the pain caused substances to

relieve pain.

Epidermal growth factor( EGF): EGF is a multifunctional growth factor which has

strong mitogenic effect of various tissue cells both in vivo and in vitro.

Page 90: Treatment Clinical Reports Zhoulin Bio Spectrum

Treatment of frostbite,mild burns,bedsores,hemorrhoids, leg ulcers, trauma

Location of irradiation:

the affected part and acupuncture point: 12

Number of times and time:

1-2 times/day

30-40 min/time

Acupoint for frostbite, trauma and

ulcers

Page 91: Treatment Clinical Reports Zhoulin Bio Spectrum

VI. Zhoulin BFS can lower blood glucose levels

Page 92: Treatment Clinical Reports Zhoulin Bio Spectrum

Study on regulation of animal's blood glucose levels by WS series

spectrum treatment device

Li Wenhui Wang Wanyu Zhu Shaowen

Kunming Institute of Zoology, Chinese Academy of Sciences

Xiong Hai Zhou Lin

Biomedical Engineering Institute of Yunan

Article 1

Page 93: Treatment Clinical Reports Zhoulin Bio Spectrum

Simple experiment principles

Damage the pancreas of the rats with alloxan to make a diabetes

model. In the meanwhile, we use hypoglycemic drug Metformin, or

WS series spectrum treatment device for treatment; Compare the

difference of the experiment and draw conclusions.

Alloxan Group, BFS Group, Metformin Group, BFS+Metformin

Group , Control Group

Page 94: Treatment Clinical Reports Zhoulin Bio Spectrum

Study on regulation of animal's blood glucose levels by WS

series spectrum treatment device

Page 95: Treatment Clinical Reports Zhoulin Bio Spectrum

Conclusion

WS series spectrum treatment device can not only significantly lower blood glucose

levels but also lower the high cholesterol and enhance the activity of superoxide

dismutase (SOD). However, the hypoglycemic effect is not as good as Metformin

group. It and can speed up the absorption of metformin.

WS series spectrum treatment device can promote the absorption of hypoglycemic

drugs. It can have synergistic effects with the drugs to reduce the use of

hypoglycemic drugs.

WS series spectrum treatment device can lower blood glucose levels and to

promote absorption of hypoglycemic drugs.

Page 96: Treatment Clinical Reports Zhoulin Bio Spectrum

Observation of Zhoulin Bio-spectrum treatment Device

on regulation of blood glucose for patients with diabetes

Feng Jinguang Qian Rongli

Department of Endocrinology, First Hospital of Beijing Medical University,

Zhang Renling Xing weichang

Department of Endocrinology, Xuanwu Hospital, Capital Medical College

Article 2

Page 97: Treatment Clinical Reports Zhoulin Bio Spectrum

Group A change of blood glucose level

before and after treatment

Based on their regular diet and regular hypoglycemic medicine, 74 cases of old and new

patients with type Ⅱ diabetes also use Zhoulin BFS Device for auxiliary treatment. The

fasting blood glucose of the two groups of patients were significantly decreased. But it raised

again after two weeks of stopping irradiation. But again, the blood glucose decreased further

when continuing with the irradiation. The glycated hemoglobin was significantly decreased

after irradiation. However, we need further observation and study on the mechanism of its

hypoglycemic effect and long-term hypoglycemic effects.

Group B change of blood glucose level

before and after treatment

Page 98: Treatment Clinical Reports Zhoulin Bio Spectrum

It can lower blood glucose Significantly.

It can lower the blood lipids.

It can promote the absorption of hypoglycemic drugs.

It can have synergistic effects with hypoglycemic drugs.

Effect of Zhoulin BFS Device on Blood glucose

Page 99: Treatment Clinical Reports Zhoulin Bio Spectrum

Hypoglycemic effect

Location of irradiation:

Acupuncture point: 7

number of times and time:

2 times/day

30 min./time

Acupoint to lower the blood

glucose level

Page 100: Treatment Clinical Reports Zhoulin Bio Spectrum

Diseases Mechanism

ischemic disease such as coronary heart disease and

cervical vertebra hyperosteogeny

improve blood rheology

increase red blood cell deformability

promote blood circulation

cervical spondylosis, lumbar hyperosteogeny, lumbar

and leg pain, facial paralysis,etc

improve circulation

ease neural stem edema

repair medullary sheath

chronic gastritis, gastric ulcers, duodenal ulcers improve blood flow of membrance

promote recovery of tissues

wound recovery, burns, frostbite, bedsore,etc promote local circulation

inhibit the increase of vascular permeability

promote the activity of fibroblast growth factor (GFs)

increase collagen synthesis

bronchitis, paediatric pneumonia, respiratory infections,

bronchial asthma,etc

promote immunity

reduce tissue edema

improve blood circulation

auxiliary treatment with antibiotics

diabetes, diabetes mellitus complicated by vascular

lesion, diabetic foot, etc.

lower blood glucose

lower blood lipids

promote blood circulation of the lower limbs

accelerate recovery of the wound

Treatment mechanism of several diseases

Page 101: Treatment Clinical Reports Zhoulin Bio Spectrum

We can draw the conclucion from above: besides thermal effects, the unique

low-energy non-thermal effects produced by Zhoulin Bio-spectrum Treatment

Device has been verified by a large number of biological experiments and

clinical applications. This is the key difference between Zhoulin products and

other TDP products.

Page 102: Treatment Clinical Reports Zhoulin Bio Spectrum

Thank you!