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TROBLESHOOTING HIGH PERFORMANCE LIQIUD CHROMATOGRAPHY HPLC
Reza Daj6/21/2014
Today’s Overview •Introduction1•Troubleshooting Approach2•Pressure Problems3•Leaks4•Component Malfunctions5•Baseline Problems6
•Peak Problems7
•Data Problems8
Introduction
TROUBLESHOOTING APPROACH
Do It Yourself
Reference Points
Common Problems
SafetyGetting the
FactsIsolating
the Sourcerepair
Strategy
Preventive Maintenanc
e
Pressure Problems
Pressure Problems
High Pressure
Low Pressure Locating a Blockage
Erratic or Cycling Pressure
Leaks
Leaks
Location the source
Pump LeaksTube fitting Leaks
Detector Leaks
Component Malfunctions
Component
Malfunctions
Electronic Problems
Injector Problems
Column Problems
Pump and Gradient
Detector Problems
BASELINE PROBLEMSNoisy Baselines1. Pump2. Gradient3. Detector4. Recorder5. Data SystemDrifting or Cycling Baseline1. Equilibration2. Late Peaks3. Mixer4. Temperature5. Electrical6. Gradient7. Cell Problem
PEAK PROBLEMS
•No Peak or Small Peaks1•Bad Peak Shape2•Extra Peak3•Negative Peak4•Incorrect Retention Time5•Poor Resolution6
