Troubleshooting frozen sections: How to achieve the perfect section Ronda W 1 , Mills V 1 , Henderson L 1 , Taggart G 1 and Myint E 1 1. Histopathology Department, Douglass Hanly Moir Pathology, Macquarie Park, Sydney, Australia Introduction To perform rapid microscopic analysis of a specimen for diagnosis by frozen section is essential in almost all major surgeries. Without a proper diagnosable section it is impossible for the pathologist to render a report and it will not be helpful for the surgeons to proceed with the procedure. This guide is intended to provide the scientists and pathologists who perform frozen sections with some useful troubleshooting techniques. Conclusion Achieving the perfect frozen section is a conglomeration of multiple factors, from the amount and positioning of OCT, blade sharpness, tightening screws being secured and the cryochamber being set to the correct temperature for that tissue type. References 1. Evans, C. Intraoperative diagnosis using the frozen section technique. J Clin Pathol. 2006 Mar; 59(3): 334 2. Bancroft, J. Theory and practice of histological techniques. 5th edn. Edinburgh: Churchill Livingstone, 2002 3. Underwood, J. Introduction to biopsy interpretation and surgical pathology. 2nd ed. Berlin:Springer-Verlag 1987 Embedding tissue Depending on the size of tissue, a small amount of OCT is considered optimal for achieving a perfect section, as it reduces the surface area of the mould coming into contact with the blade. Chatter during sectioning Orientate specimen so that the smaller surface area is coming into contact with blade. Sections not forming/sticking to blade surface Apply cryospray to the blade, OCT mould and brush. Sections curling Gently press your thumb against OCT mould to increase temperature to prevent curling. VS ✔ ✗ ✗ ✔

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Page 1: Troubleshooting frozen sections: How to achieve the

Troubleshooting frozen sections: How to achieve the perfect section

Ronda W1, Mills V1, Henderson L1, Taggart G1 and Myint E1

1. Histopathology Department, Douglass Hanly Moir Pathology, Macquarie Park, Sydney, Australia

IntroductionTo perform rapid microscopic analysis of a specimen for diagnosis by frozen section is essential in almost all major surgeries. Without a proper diagnosable section it is impossible for the pathologist to

render a report and it will not be helpful for the surgeons to proceed with the procedure. This guide is intended to provide the scientists

and pathologists who perform frozen sections with some useful troubleshooting techniques.

ConclusionAchieving the perfect frozen section is a conglomeration of multiple factors, from the amount and positioning of OCT, blade sharpness, tightening screws being secured and the cryochamber being set to the correct temperature for that tissue type.

References1. Evans, C. Intraoperative diagnosis using the frozen section technique. J Clin Pathol. 2006 Mar; 59(3): 3342. Bancroft, J. Theory and practice of histological techniques. 5th edn. Edinburgh: Churchill Livingstone, 20023. Underwood, J. Introduction to biopsy interpretation and surgical pathology. 2nd ed. Berlin:Springer-Verlag 1987

Embedding tissueDepending on the size of tissue, a small amount of OCT is considered optimal for achieving a perfect section, as it reduces the surface area of the mould coming into contact with the blade.

Chatter during sectioning

Orientate specimen so that the smaller surface area is coming into contact with blade.

Sections not forming/sticking to blade surfaceApply cryospray to the blade, OCT mould and brush.

Sections curling Gently press your thumb against OCT mould to increase temperature to prevent curling.

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