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New serologic diagnosed test for typhoid fever

TUBEX detects anti-Salmonella O9 (immunoglobulin M [IgM]) antibodies

High sensitivity and high specifity80,36 % positive in 7-14 days fever

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Coloured indicator latex particle carrying anti-O9 monoclonal antibodies

Magnetic particle coated with S. Typhi LPS (lipopolysacharide)

Those two particle binds and sedimented leave the redish behind (red latex particle)

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If anti-O9 antibodies (+) in the reaction mixture inhibit the binding of the blue particles to the magnetic particles Consequently, the supernatant remains purplish blue (unchanged from the beginning) due to the presence of blue particles and also, in a lower concentration, of red particles.

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10 8 6 4 2 0

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KUANTITATIFHASIL POSITIF BILA > +4

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