TUE 4/23 week-6

TOPIC: REVIEW BIO-TECH OBJ: 10

DO NOW:

EXT: -- DUE DATE: --

DW: ---

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EXIT:CIRCLE-level of understanding of today’s objective

[see slide]

HANDOUTS to PICK-UP:

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TURN IN to ABS box:

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SEMINAR 2:-

BEFORE/AFTER SCHOOL:-DW:

-FINISH OUR EARTH video

-REV 13.2 APP NTS

-REV 13.2 VIRTUAL LAB

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NONE

GENERAL

FINISH OUR PLANET [15m]

13.2 APP NTS / 13.2 VIRTUAL LAB

RED PEN OUT FOR REVIEW

MAIN IDEA

Genetic engineering manipulates DNA

VOCABULARY

REVIEW DNA:

-genetic material of all organisms

-complementary double helix

VOCABULARY

IDENTIFY the terms that fit the definitions:

1-method of manipulating DNA from one organisms and inserting the DNA fragment

into a host organisms of the same/different species

-GENETIC ENGINEERING

2-the total DNA present in the nucleus of each cell

-GENOME

3-bacterial enzyme that can cut foreign DNA at a specific nucleotide sequence

-RESTRICTION ENZYME

VOCABULARY

IDENTIFY the terms that fit the definitions:

4-a method of separating DNA fragments by size with the use of an electric current

-GEL ELECTROPHORESIS

5-DNA made by recombining fragments of DNA from different sources

-RECOMBINANT DNA

6-an enzyme that is used to join DNA fragments; used by the cell for DNA repair and

replication

-DNA LIGASE

7-the process of creating a genetically identical copy of an organism or gene

-CLONING

GENETIC ENGINEERING

IDENTIFY the terms that complete the phrase below.

-Selective breeding produces organisms with _________________ ,

while genetic engineering actually changes how a specific _________ is

__________ in an organism’s offspring.

DESIRED TRAITS

GENE

EXPRESSED

DNA TOOLS

COMPLETE the paragraph about DNA tools by using the word bank provided

-Scientist use ____________________ to cut DNA at specific sequences

and ____________________ to separate fragments based on size.

-Some ____________________ create DNA with single-stranded,

____________________, ____________________ is an example of this

type of enzyme.

-The resulting DNA fragments can be joined with other DNA fragments that

have complementary ___________________.

-Other _________________________ create ____________________,

which can be joined to another DNA fragment that has

_________________________.

RESTRICTION ENZYMES

GEL ELECTROPHORESIS

RESTRICTION ENZYMES

STICKY ENDS ECO RI

STICKY ENDS

BLUNT ENDS

BLUNT ENDS

RESTRICTION ENZYMES

RECOMBINANT DNA TECHNOLOGY

COMPARE the DNA tools and techniques used in genetic engineering

-PROTECT function

of gene

-COMPARE similar genes

among organisms

-IDENTIFY mutations

-MAKE millions of

COPIES of a specific

region of DNA fragment

for STUDY

REVIEW

1- An organism’s ___ is the total DNA present in the nucleus of each cell.

genome

2- A ___ can recognize, bind, and cut a specific DNA sequence

restriction enzyme

3- This shows the cleavage of a DNA sequence. This occurs ___ the

forming of recombinant DNA.

during

REVIEW

4- What process separates DNA fragments according to size?

gel electrophoresis

5- What is the result of a polymerase chain reaction?

millions of replicated DNA fragments

SUMMARIZE

(5 bulleted sentences) the uses of genetic technology

-POLYMERASE CHAIN REACTION

-used to establish paternity

-identify victims and suspects in a crime

-detect infectious disease

-GENETIC TECHNOLOGY

-used to create organisms for MEDICAL use

-used to create organisms for AGRICULTURAL USE

ELECTROPHORESIS VIRTUAL LAB

ELECTROPHORESIS VIRTUAL LAB

GEL ELECTROPHORESIS1-What is your job?

-determine lengths of DNA

2-IDENTIFY the FUNCTION of GEL ELECTROPHORESIS?

-SORT & MEASURE DNA strands by length

3-How does GE work?

-gel acts as a filter

-separates molecules (electrical charge)

-sorts DNA strands (banded pattern)

SEQUENCE steps of DNA moving thru gel

GEL ELECTROPHORESIS4-Do LONG or SHORT strands move more quickly thru the gel?

-SHORT

5-Can we see single strands of DNA in the gel?

-NO—they are too small even under a microscope

GEL ELECTROPHORESIS6-LIST 5 basic steps of GE

-______________________________________

-______________________________________

-______________________________________

-______________________________________

-______________________________________

MODEL 5 steps of GE with picture procedures

Make gel

Set up apparatus

Place DNA samples in holes at end of gel

ADD electric current

Stain-ANALYZE

GEL ELECTROPHORESISWALK THRU LAB

7-DETERMINE what each is made of

AGAROSE: -seaweed

BUFFER: -salt water solution

8-why do buffer & agarose need to be heated?

-melt agarose into buffer

9-why does the mold have tape on each end?

-holds melted agarose

10-IDENTIFY why a COMB placed in the gel at one end

-makes “wells” (small holes) to hold DNA samples

11-ANALYZE 2 reasons buffer solution is added to GE box

-conducts electric current

-keeps gel from drying out

GEL ELECTROPHORESIS12-DETERMINE 2 functions of loading buffer

-contains a dye making sample easy to see

-makes DNA sample thicker so it doesn’t float away

13-PREDICT in a real lab difficulties loading samples

-NOT easy—might take some practice

14-why would we use a standard DNA size sample?

-contains DNA strands of known lengths

-provides reference to estimate lengths of DNA strands

15-CONCLUDE why electricity is used to run the wells

-DNA negative charge attracted to + charge

GEL ELECTROPHORESIS16-how can you check to make sure your current is running?

-should see tiny bubbles

17-does DNA move to + or – end?

-+

18-why is the gel stained and how does it work?

-cannot see DNA but can see dyes

-can see large groups of stained DNA strands

-dye binds to DNA-shows up under flouresent lights

GEL ELECTROPHORESIS19-ENTER bp— base pairs —size estimates

-6000

-3500

-1500

EXITCIRCLE-level of understanding of today’s objective

Q’s / clarifications-EXIT--COMPLETE OBJ 10